                         SEQUENCE LISTING

<110>  Thermo Fisher Scientific Baltics UAB
 
<120>  Improved Deoxyribonuclease Enzymes

<130>  335422PCT/CJS

<150>  GB 1409709.1
<151>  2014-06-02

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  873
<212>  DNA
<213>  Bos taurus

<400>  1
atggcgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcgct ccggtgatga tgatgacaag ctgaagatag cagccttcaa catccgcacc      120

tttggggaga ccaagatgtc caatgctacg ctcgccagct acattgttcg gatcgtgcgt      180

cgttacgaca tcgtcctcat ccaggaggtc agagacagcc acctggtggc tgtggggaag      240

ctcctggact atctcaacca ggatgaccca aacacctacc actatgtggt cagtgagccg      300

ctgggccgca acagctacaa ggagcgctac ctctttctgt tcagacccaa caaggtgtcc      360

gtgctggaca cctaccagta cgacgacggc tgcgagtcct gcgggaacga cagcttcagc      420

cgggagcccg ctgtggtcaa gttctcatcc cactccacca aggtcaagga atttgccatt      480

gttgccctgc actcggcccc atcggacgca gtggctgaga ttaattctct ctacgatgtc      540

tacctggatg tccagcagaa gtggcacttg aacgatgtca tgttgatggg cgatttcaat      600

gctgactgca gctacgtgac ctcctcgcag tggtcatcca tccgcctgcg tacgagctcc      660

accttccagt ggctgattcc tgacagtgcc gacaccacgg ctacgtccac gaactgcgcc      720

tatgacagga tcgtggtcgc agggtctctg ctccagagtt ctgtggttcc tggctcggcc      780

gctccctttg acttccaagc tgcatacgga ctgagcaatg agatggccct ggccatcagt      840

gaccattacc cggtggaggt gacgctgaca taa                                   873


<210>  2
<211>  290
<212>  PRT
<213>  Bos taurus

<400>  2

Met Ala Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Ser Gly Asp Asp Asp Asp Lys Leu Lys 
            20                  25                  30          


Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met Ser Asn 
        35                  40                  45              


Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Val Arg Arg Tyr Asp Ile 
    50                  55                  60                  


Val Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val Gly Lys 
65                  70                  75                  80  


Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His Tyr Val 
                85                  90                  95      


Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr Leu Phe 
            100                 105                 110         


Leu Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln Tyr Asp 
        115                 120                 125             


Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu Pro Ala 
    130                 135                 140                 


Val Val Lys Phe Ser Ser His Ser Thr Lys Val Lys Glu Phe Ala Ile 
145                 150                 155                 160 


Val Ala Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile Asn Ser 
                165                 170                 175     


Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu Asn Asp 
            180                 185                 190         


Val Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Thr Ser 
        195                 200                 205             


Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe Gln Trp 
    210                 215                 220                 


Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn Cys Ala 
225                 230                 235                 240 


Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser Val Val 
                245                 250                 255     


Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly Leu Ser 
            260                 265                 270         


Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu Val Thr 
        275                 280                 285             


Leu Thr 
    290 


<210>  3
<211>  1038
<212>  DNA
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  3
atgctgcgcc tggcaagctg gaacatccag cacctgggct ggaacgtcgg taaggactac       60

ccggccgtcg cacgcatcgc ggcgcagttc gactttctgg cgatccaaga agtcatgaac      120

gccgagggta tttaccgcct gcgtgacacc ctggaagacg cgaccggtgc cgagtggtcc      180

gttctgtact ccgacgcgct gggccgcaac acctatcgtg aaaagtacgc gttcctgtgg      240

cgtgaggccg cagttgagta tgtcggtggt gcgctgacct acatcgacga ggccgaccgt      300

tttgcccgtg agccgttctc cgcggtcttt cgttcccgtg gcacggatca gcatttcctg      360

gccgccacgg ttcacatcac ctacggcgac cgtgttgcgg atcgtgtcga ggagatcgag      420

gcactgcgtc gttactggga ctggctggcg gacgtcatgc cggagtacgc cggcgaacgc      480

atcctgttcg gcgacttcaa tctgccgccg caccacgacg gttgggcctc gatgcgtgcg      540

gttgccgaac cgctggtcac cgagggcgcc accaccctgt ccacgcatga ccgtcgctac      600

gcgaatctgt acgacaatct gtgggttccg aaggatcaca cgctgccgct gggtgatgcc      660

ggcatcctgc cgttcccggt cgttctgagt gaggtaaccg gtgtctactg ggatcacgaa      720

aaggcccgcg accgtgtctc cgaccatgcc ccggtttatg tactgttcga aggtaacacg      780

ctgcacgacg cggttgtcgc ggagatcgcc gatcaagaag cgggctgcat cgatctgaat      840

cgcgcctcgg taagcgaact gaccgccctg ccgcatattg gcgaggcacg tgcggaggcg      900

atcaaggacg gtcgtccgtg gaatgccgtt cgtgacctga aagagattcg cggtatcggt      960

gctggtcgtc tggaggagat caaggcgcgt ggtgaggcct gcatcgagcc gggccatcac     1020

catcaccacc acggctaa                                                   1038


<210>  4
<211>  345
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  4

Met Leu Arg Leu Ala Ser Trp Asn Ile Gln His Leu Gly Trp Asn Val 
1               5                   10                  15      


Gly Lys Asp Tyr Pro Ala Val Ala Arg Ile Ala Ala Gln Phe Asp Phe 
            20                  25                  30          


Leu Ala Ile Gln Glu Val Met Asn Ala Glu Gly Ile Tyr Arg Leu Arg 
        35                  40                  45              


Asp Thr Leu Glu Asp Ala Thr Gly Ala Glu Trp Ser Val Leu Tyr Ser 
    50                  55                  60                  


Asp Ala Leu Gly Arg Asn Thr Tyr Arg Glu Lys Tyr Ala Phe Leu Trp 
65                  70                  75                  80  


Arg Glu Ala Ala Val Glu Tyr Val Gly Gly Ala Leu Thr Tyr Ile Asp 
                85                  90                  95      


Glu Ala Asp Arg Phe Ala Arg Glu Pro Phe Ser Ala Val Phe Arg Ser 
            100                 105                 110         


Arg Gly Thr Asp Gln His Phe Leu Ala Ala Thr Val His Ile Thr Tyr 
        115                 120                 125             


Gly Asp Arg Val Ala Asp Arg Val Glu Glu Ile Glu Ala Leu Arg Arg 
    130                 135                 140                 


Tyr Trp Asp Trp Leu Ala Asp Val Met Pro Glu Tyr Ala Gly Glu Arg 
145                 150                 155                 160 


Ile Leu Phe Gly Asp Phe Asn Leu Pro Pro His His Asp Gly Trp Ala 
                165                 170                 175     


Ser Met Arg Ala Val Ala Glu Pro Leu Val Thr Glu Gly Ala Thr Thr 
            180                 185                 190         


Leu Ser Thr His Asp Arg Arg Tyr Ala Asn Leu Tyr Asp Asn Leu Trp 
        195                 200                 205             


Val Pro Lys Asp His Thr Leu Pro Leu Gly Asp Ala Gly Ile Leu Pro 
    210                 215                 220                 


Phe Pro Val Val Leu Ser Glu Val Thr Gly Val Tyr Trp Asp His Glu 
225                 230                 235                 240 


Lys Ala Arg Asp Arg Val Ser Asp His Ala Pro Val Tyr Val Leu Phe 
                245                 250                 255     


Glu Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp Gln 
            260                 265                 270         


Glu Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val Ser Glu Leu Thr 
        275                 280                 285             


Ala Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala Ile Lys Asp Gly 
    290                 295                 300                 


Arg Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile Arg Gly Ile Gly 
305                 310                 315                 320 


Ala Gly Arg Leu Glu Glu Ile Lys Ala Arg Gly Glu Ala Cys Ile Glu 
                325                 330                 335     


Pro Gly His His His His His His Gly 
            340                 345 


<210>  5
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gccgccacgg ttgcaatcac ctacgg                                            26


<210>  6
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
gtggtggtga tggtgatggc ccggctcgat gcaggcctca c                           41


<210>  7
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
agaaggagat ataactatgc tgcgcctggc aagctgg                                37


<210>  8
<211>  1038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of DNaseTA H134A mutant

<400>  8
atgctgcgcc tggcaagctg gaacatccag cacctgggct ggaacgtcgg taaggactac       60

ccggccgtcg cacgcatcgc ggcgcagttc gactttctgg cgatccaaga agtcatgaac      120

gccgagggta tttaccgcct gcgtgacacc ctggaagacg cgaccggtgc cgagtggtcc      180

gttctgtact ccgacgcgct gggccgcaac acctatcgtg aaaagtacgc gttcctgtgg      240

cgtgaggccg cagttgagta tgtcggtggt gcgctgacct acatcgacga ggccgaccgt      300

tttgcccgtg agccgttctc cgcggtcttt cgttcccgtg gcacggatca gcatttcctg      360

gccgccacgg ttgcaatcac ctacggcgac cgtgttgcgg atcgtgtcga ggagatcgag      420

gcactgcgtc gttactggga ctggctggcg gacgtcatgc cggagtacgc cggcgaacgc      480

atcctgttcg gcgacttcaa tctgccgccg caccacgacg gttgggcctc gatgcgtgcg      540

gttgccgaac cgctggtcac cgagggcgcc accaccctgt ccacgcatga ccgtcgctac      600

gcgaatctgt acgacaatct gtgggttccg aaggatcaca cgctgccgct gggtgatgcc      660

ggcatcctgc cgttcccggt cgttctgagt gaggtaaccg gtgtctactg ggatcacgaa      720

aaggcccgcg accgtgtctc cgaccatgcc ccggtttatg tactgttcga aggtaacacg      780

ctgcacgacg cggttgtcgc ggagatcgcc gatcaagaag cgggctgcat cgatctgaat      840

cgcgcctcgg taagcgaact gaccgccctg ccgcatattg gcgaggcacg tgcggaggcg      900

atcaaggacg gtcgtccgtg gaatgccgtt cgtgacctga aagagattcg cggtatcggt      960

gctggtcgtc tggaggagat caaggcgcgt ggtgaggcct gcatcgagcc gggccatcac     1020

catcaccacc acggctaa                                                   1038


<210>  9
<211>  345
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DNaseTA H134A mutant

<400>  9

Met Leu Arg Leu Ala Ser Trp Asn Ile Gln His Leu Gly Trp Asn Val 
1               5                   10                  15      


Gly Lys Asp Tyr Pro Ala Val Ala Arg Ile Ala Ala Gln Phe Asp Phe 
            20                  25                  30          


Leu Ala Ile Gln Glu Val Met Asn Ala Glu Gly Ile Tyr Arg Leu Arg 
        35                  40                  45              


Asp Thr Leu Glu Asp Ala Thr Gly Ala Glu Trp Ser Val Leu Tyr Ser 
    50                  55                  60                  


Asp Ala Leu Gly Arg Asn Thr Tyr Arg Glu Lys Tyr Ala Phe Leu Trp 
65                  70                  75                  80  


Arg Glu Ala Ala Val Glu Tyr Val Gly Gly Ala Leu Thr Tyr Ile Asp 
                85                  90                  95      


Glu Ala Asp Arg Phe Ala Arg Glu Pro Phe Ser Ala Val Phe Arg Ser 
            100                 105                 110         


Arg Gly Thr Asp Gln His Phe Leu Ala Ala Thr Val Ala Ile Thr Tyr 
        115                 120                 125             


Gly Asp Arg Val Ala Asp Arg Val Glu Glu Ile Glu Ala Leu Arg Arg 
    130                 135                 140                 


Tyr Trp Asp Trp Leu Ala Asp Val Met Pro Glu Tyr Ala Gly Glu Arg 
145                 150                 155                 160 


Ile Leu Phe Gly Asp Phe Asn Leu Pro Pro His His Asp Gly Trp Ala 
                165                 170                 175     


Ser Met Arg Ala Val Ala Glu Pro Leu Val Thr Glu Gly Ala Thr Thr 
            180                 185                 190         


Leu Ser Thr His Asp Arg Arg Tyr Ala Asn Leu Tyr Asp Asn Leu Trp 
        195                 200                 205             


Val Pro Lys Asp His Thr Leu Pro Leu Gly Asp Ala Gly Ile Leu Pro 
    210                 215                 220                 


Phe Pro Val Val Leu Ser Glu Val Thr Gly Val Tyr Trp Asp His Glu 
225                 230                 235                 240 


Lys Ala Arg Asp Arg Val Ser Asp His Ala Pro Val Tyr Val Leu Phe 
                245                 250                 255     


Glu Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp Gln 
            260                 265                 270         


Glu Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val Ser Glu Leu Thr 
        275                 280                 285             


Ala Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala Ile Lys Asp Gly 
    290                 295                 300                 


Arg Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile Arg Gly Ile Gly 
305                 310                 315                 320 


Ala Gly Arg Leu Glu Glu Ile Lys Ala Arg Gly Glu Ala Cys Ile Glu 
                325                 330                 335     


Pro Gly His His His His His His Gly 
            340                 345 


<210>  10
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
gtggtggtga tggtgatggc caccttcgaa cagtacataa accgg                       45


<210>  11
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
agaaggagat ataactatgc tgcgcctggc aagc                                   34


<210>  12
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of DNaseTA deletion mutant

<400>  12
atgctgcgcc tggcaagctg gaacatccag cacctgggct ggaacgtcgg taaggactac       60

ccggccgtcg cacgcatcgc ggcgcagttc gactttctgg cgatccaaga agtcatgaac      120

gccgagggta tttaccgcct gcgtgacacc ctggaagacg cgaccggtgc cgagtggtcc      180

gttctgtact ccgacgcgct gggccgcaac acctatcgtg aaaagtacgc gttcctgtgg      240

cgtgaggccg cagttgagta tgtcggtggt gcgctgacct acatcgacga ggccgaccgt      300

tttgcccgtg agccgttctc cgcggtcttt cgttcccgtg gcacggatca gcatttcctg      360

gccgccacgg ttcacatcac ctacggcgac cgtgttgcgg atcgtgtcga ggagatcgag      420

gcactgcgtc gttactggga ctggctggcg gacgtcatgc cggagtacgc cggcgaacgc      480

atcctgttcg gcgacttcaa tctgccgccg caccacgacg gttgggcctc gatgcgtgcg      540

gttgccgaac cgctggtcac cgagggcgcc accaccctgt ccacgcatga ccgtcgctac      600

gcgaatctgt acgacaatct gtgggttccg aaggatcaca cgctgccgct gggtgatgcc      660

ggcatcctgc cgttcccggt cgttctgagt gaggtaaccg gtgtctactg ggatcacgaa      720

aaggcccgcg accgtgtctc cgaccatgcc ccggtttatg tactgttcga aggtggccat      780

caccatcacc accacggcta a                                                801


<210>  13
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DNaseTA deletion mutant

<400>  13

Met Leu Arg Leu Ala Ser Trp Asn Ile Gln His Leu Gly Trp Asn Val 
1               5                   10                  15      


Gly Lys Asp Tyr Pro Ala Val Ala Arg Ile Ala Ala Gln Phe Asp Phe 
            20                  25                  30          


Leu Ala Ile Gln Glu Val Met Asn Ala Glu Gly Ile Tyr Arg Leu Arg 
        35                  40                  45              


Asp Thr Leu Glu Asp Ala Thr Gly Ala Glu Trp Ser Val Leu Tyr Ser 
    50                  55                  60                  


Asp Ala Leu Gly Arg Asn Thr Tyr Arg Glu Lys Tyr Ala Phe Leu Trp 
65                  70                  75                  80  


Arg Glu Ala Ala Val Glu Tyr Val Gly Gly Ala Leu Thr Tyr Ile Asp 
                85                  90                  95      


Glu Ala Asp Arg Phe Ala Arg Glu Pro Phe Ser Ala Val Phe Arg Ser 
            100                 105                 110         


Arg Gly Thr Asp Gln His Phe Leu Ala Ala Thr Val His Ile Thr Tyr 
        115                 120                 125             


Gly Asp Arg Val Ala Asp Arg Val Glu Glu Ile Glu Ala Leu Arg Arg 
    130                 135                 140                 


Tyr Trp Asp Trp Leu Ala Asp Val Met Pro Glu Tyr Ala Gly Glu Arg 
145                 150                 155                 160 


Ile Leu Phe Gly Asp Phe Asn Leu Pro Pro His His Asp Gly Trp Ala 
                165                 170                 175     


Ser Met Arg Ala Val Ala Glu Pro Leu Val Thr Glu Gly Ala Thr Thr 
            180                 185                 190         


Leu Ser Thr His Asp Arg Arg Tyr Ala Asn Leu Tyr Asp Asn Leu Trp 
        195                 200                 205             


Val Pro Lys Asp His Thr Leu Pro Leu Gly Asp Ala Gly Ile Leu Pro 
    210                 215                 220                 


Phe Pro Val Val Leu Ser Glu Val Thr Gly Val Tyr Trp Asp His Glu 
225                 230                 235                 240 


Lys Ala Arg Asp Arg Val Ser Asp His Ala Pro Val Tyr Val Leu Phe 
                245                 250                 255     


Glu Gly Gly His His His His His His Gly 
            260                 265     


<210>  14
<211>  237
<212>  DNA
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  14
ggtaacacgc tgcacgacgc ggttgtcgcg gagatcgccg atcaagaagc gggctgcatc       60

gatctgaatc gcgcctcggt aagcgaactg accgccctgc cgcatattgg cgaggcacgt      120

gcggaggcga tcaaggacgg tcgtccgtgg aatgccgttc gtgacctgaa agagattcgc      180

ggtatcggtg ctggtcgtct ggaggagatc aaggcgcgtg gtgaggcctg catcgag         237


<210>  15
<211>  79
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  15

Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp Gln Glu 
1               5                   10                  15      


Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val Ser Glu Leu Thr Ala 
            20                  25                  30          


Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala Ile Lys Asp Gly Arg 
        35                  40                  45              


Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile Arg Gly Ile Gly Ala 
    50                  55                  60                  


Gly Arg Leu Glu Glu Ile Lys Ala Arg Gly Glu Ala Cys Ile Glu 
65                  70                  75                  


<210>  16
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
cggtggaggt gacgctgaca ggtaacacgc tgcacgacgc                             40


<210>  17
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
ggagatggga agtcattact cgatgcaggc ctcaccac                               38


<210>  18
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
ggtgatgatg atgacaagct gaagatagca gccttcaaca tccg                        44


<210>  19
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
cggtggaggt gacgctgaca ggagaggaaa cagcagtgca gc                          42


<210>  20
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
ggagatggga agtcattact ttactgtaat ggaagacttt attttctca                   49


<210>  21
<211>  255
<212>  DNA
<213>  Bacillus subtilis (strain 168)

<400>  21
ggagaggaaa cagcagtgca gcaaggtggc ggagggtctg tccaaagcga tggagggaag       60

ggagcgctgg tgaatatcaa tacagcaacc ttagaggagt tacaaggcat ctcaggggtg      120

gggccatcca aagctgaagc tattattgca taccgggagg aaaacggtcg tttccaaaca      180

attgaagata tcactaaggt ttcaggaata ggtgaaaagt catttgagaa aataaagtct      240

tccattacag taaag                                                       255


<210>  22
<211>  85
<212>  PRT
<213>  Bacillus subtilis (strain 168)

<400>  22

Gly Glu Glu Thr Ala Val Gln Gln Gly Gly Gly Gly Ser Val Gln Ser 
1               5                   10                  15      


Asp Gly Gly Lys Gly Ala Leu Val Asn Ile Asn Thr Ala Thr Leu Glu 
            20                  25                  30          


Glu Leu Gln Gly Ile Ser Gly Val Gly Pro Ser Lys Ala Glu Ala Ile 
        35                  40                  45              


Ile Ala Tyr Arg Glu Glu Asn Gly Arg Phe Gln Thr Ile Glu Asp Ile 
    50                  55                  60                  


Thr Lys Val Ser Gly Ile Gly Glu Lys Ser Phe Glu Lys Ile Lys Ser 
65                  70                  75                  80  


Ser Ile Thr Val Lys 
                85  


<210>  23
<211>  1128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of DNaseBS

<400>  23
atggcgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcgct ccggtgatga tgatgacaag ctgaagatag cagccttcaa catccgcacc      120

tttggggaga ccaagatgtc caatgctacg ctcgccagct acattgttcg gatcgtgcgt      180

cgttacgaca tcgtcctcat ccaggaggtc agagacagcc acctggtggc tgtggggaag      240

ctcctggact atctcaacca ggatgaccca aacacctacc actatgtggt cagtgagccg      300

ctgggccgca acagctacaa ggagcgctac ctctttctgt tcagacccaa caaggtgtcc      360

gtgctggaca cctaccagta cgacgacggc tgcgagtcct gcgggaacga cagcttcagc      420

cgggagcccg ctgtggtcaa gttctcatcc cactccacca aggtcaagga atttgccatt      480

gttgccctgc actcggcccc atcggacgca gtggctgaga ttaattctct ctacgatgtc      540

tacctggatg tccagcagaa gtggcacttg aacgatgtca tgttgatggg cgatttcaat      600

gctgactgca gctacgtgac ctcctcgcag tggtcatcca tccgcctgcg tacgagctcc      660

accttccagt ggctgattcc tgacagtgcc gacaccacgg ctacgtccac gaactgcgcc      720

tatgacagga tcgtggtcgc agggtctctg ctccagagtt ctgtggttcc tggctcggcc      780

gctccctttg acttccaagc tgcatacgga ctgagcaatg agatggccct ggccatcagt      840

gaccattacc cggtggaggt gacgctgaca ggagaggaaa cagcagtgca gcaaggtggc      900

ggagggtctg tccaaagcga tggagggaag ggagcgctgg tgaatatcaa tacagcaacc      960

ttagaggagt tacaaggcat ctcaggggtg gggccatcca aagctgaagc tattattgca     1020

taccgggagg aaaacggtcg tttccaaaca attgaagata tcactaaggt ttcaggaata     1080

ggtgaaaagt catttgagaa aataaagtct tccattacag taaagtaa                  1128


<210>  24
<211>  375
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DNaseBS

<400>  24

Met Ala Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Ser Gly Asp Asp Asp Asp Lys Leu Lys 
            20                  25                  30          


Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met Ser Asn 
        35                  40                  45              


Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Val Arg Arg Tyr Asp Ile 
    50                  55                  60                  


Val Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val Gly Lys 
65                  70                  75                  80  


Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His Tyr Val 
                85                  90                  95      


Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr Leu Phe 
            100                 105                 110         


Leu Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln Tyr Asp 
        115                 120                 125             


Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu Pro Ala 
    130                 135                 140                 


Val Val Lys Phe Ser Ser His Ser Thr Lys Val Lys Glu Phe Ala Ile 
145                 150                 155                 160 


Val Ala Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile Asn Ser 
                165                 170                 175     


Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu Asn Asp 
            180                 185                 190         


Val Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Thr Ser 
        195                 200                 205             


Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe Gln Trp 
    210                 215                 220                 


Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn Cys Ala 
225                 230                 235                 240 


Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser Val Val 
                245                 250                 255     


Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly Leu Ser 
            260                 265                 270         


Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu Val Thr 
        275                 280                 285             


Leu Thr Gly Glu Glu Thr Ala Val Gln Gln Gly Gly Gly Gly Ser Val 
    290                 295                 300                 


Gln Ser Asp Gly Gly Lys Gly Ala Leu Val Asn Ile Asn Thr Ala Thr 
305                 310                 315                 320 


Leu Glu Glu Leu Gln Gly Ile Ser Gly Val Gly Pro Ser Lys Ala Glu 
                325                 330                 335     


Ala Ile Ile Ala Tyr Arg Glu Glu Asn Gly Arg Phe Gln Thr Ile Glu 
            340                 345                 350         


Asp Ile Thr Lys Val Ser Gly Ile Gly Glu Lys Ser Phe Glu Lys Ile 
        355                 360                 365             


Lys Ser Ser Ile Thr Val Lys 
    370                 375 


<210>  25
<211>  1110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Coding sequence of DNaseDT

<400>  25
atggcgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa       60

atgggtcgct ccggtgatga tgatgacaag ctgaagatag cagccttcaa catccgcacc      120

tttggggaga ccaagatgtc caatgctacg ctcgccagct acattgttcg gatcgtgcgt      180

cgttacgaca tcgtcctcat ccaggaggtc agagacagcc acctggtggc tgtggggaag      240

ctcctggact atctcaacca ggatgaccca aacacctacc actatgtggt cagtgagccg      300

ctgggccgca acagctacaa ggagcgctac ctctttctgt tcagacccaa caaggtgtcc      360

gtgctggaca cctaccagta cgacgacggc tgcgagtcct gcgggaacga cagcttcagc      420

cgggagcccg ctgtggtcaa gttctcatcc cactccacca aggtcaagga atttgccatt      480

gttgccctgc actcggcccc atcggacgca gtggctgaga ttaattctct ctacgatgtc      540

tacctggatg tccagcagaa gtggcacttg aacgatgtca tgttgatggg cgatttcaat      600

gctgactgca gctacgtgac ctcctcgcag tggtcatcca tccgcctgcg tacgagctcc      660

accttccagt ggctgattcc tgacagtgcc gacaccacgg ctacgtccac gaactgcgcc      720

tatgacagga tcgtggtcgc agggtctctg ctccagagtt ctgtggttcc tggctcggcc      780

gctccctttg acttccaagc tgcatacgga ctgagcaatg agatggccct ggccatcagt      840

gaccattacc cggtggaggt gacgctgaca ggtaacacgc tgcacgacgc ggttgtcgcg      900

gagatcgccg atcaagaagc gggctgcatc gatctgaatc gcgcctcggt aagcgaactg      960

accgccctgc cgcatattgg cgaggcacgt gcggaggcga tcaaggacgg tcgtccgtgg     1020

aatgccgttc gtgacctgaa agagattcgc ggtatcggtg ctggtcgtct ggaggagatc     1080

aaggcgcgtg gtgaggcctg catcgagtaa                                      1110


<210>  26
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DNaseDT

<400>  26

Met Ala Gly Ser His His His His His His Gly Met Ala Ser Met Thr 
1               5                   10                  15      


Gly Gly Gln Gln Met Gly Arg Ser Gly Asp Asp Asp Asp Lys Leu Lys 
            20                  25                  30          


Ile Ala Ala Phe Asn Ile Arg Thr Phe Gly Glu Thr Lys Met Ser Asn 
        35                  40                  45              


Ala Thr Leu Ala Ser Tyr Ile Val Arg Ile Val Arg Arg Tyr Asp Ile 
    50                  55                  60                  


Val Leu Ile Gln Glu Val Arg Asp Ser His Leu Val Ala Val Gly Lys 
65                  70                  75                  80  


Leu Leu Asp Tyr Leu Asn Gln Asp Asp Pro Asn Thr Tyr His Tyr Val 
                85                  90                  95      


Val Ser Glu Pro Leu Gly Arg Asn Ser Tyr Lys Glu Arg Tyr Leu Phe 
            100                 105                 110         


Leu Phe Arg Pro Asn Lys Val Ser Val Leu Asp Thr Tyr Gln Tyr Asp 
        115                 120                 125             


Asp Gly Cys Glu Ser Cys Gly Asn Asp Ser Phe Ser Arg Glu Pro Ala 
    130                 135                 140                 


Val Val Lys Phe Ser Ser His Ser Thr Lys Val Lys Glu Phe Ala Ile 
145                 150                 155                 160 


Val Ala Leu His Ser Ala Pro Ser Asp Ala Val Ala Glu Ile Asn Ser 
                165                 170                 175     


Leu Tyr Asp Val Tyr Leu Asp Val Gln Gln Lys Trp His Leu Asn Asp 
            180                 185                 190         


Val Met Leu Met Gly Asp Phe Asn Ala Asp Cys Ser Tyr Val Thr Ser 
        195                 200                 205             


Ser Gln Trp Ser Ser Ile Arg Leu Arg Thr Ser Ser Thr Phe Gln Trp 
    210                 215                 220                 


Leu Ile Pro Asp Ser Ala Asp Thr Thr Ala Thr Ser Thr Asn Cys Ala 
225                 230                 235                 240 


Tyr Asp Arg Ile Val Val Ala Gly Ser Leu Leu Gln Ser Ser Val Val 
                245                 250                 255     


Pro Gly Ser Ala Ala Pro Phe Asp Phe Gln Ala Ala Tyr Gly Leu Ser 
            260                 265                 270         


Asn Glu Met Ala Leu Ala Ile Ser Asp His Tyr Pro Val Glu Val Thr 
        275                 280                 285             


Leu Thr Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp 
    290                 295                 300                 


Gln Glu Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val Ser Glu Leu 
305                 310                 315                 320 


Thr Ala Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala Ile Lys Asp 
                325                 330                 335     


Gly Arg Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile Arg Gly Ile 
            340                 345                 350         


Gly Ala Gly Arg Leu Glu Glu Ile Lys Ala Arg Gly Glu Ala Cys Ile 
        355                 360                 365             


Glu 
    


<210>  27
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide


<220>
<221>  MISC_FEATURE
<223>  3' BHQ1

<400>  27

Gly Thr Thr Gly Gly Thr Gly Gly Gly Thr Thr Thr Gly Gly Gly Thr 
1               5                   10                  15      


Gly Thr Gly Gly Gly Thr Thr Thr Gly Thr Gly Thr Thr Thr 
            20                  25                  30  


<210>  28
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide


<220>
<221>  MISC_FEATURE
<223>  5' FAM

<400>  28

Ala Ala Ala Cys Ala Cys Ala Ala Ala Cys Cys Cys Ala Cys Ala Cys 
1               5                   10                  15      


Cys Cys Ala Ala Ala Cys Cys Cys Ala Cys Cys Ala Ala Cys 
            20                  25                  30  


<210>  29
<211>  20
<212>  PRT
<213>  Bacillus subtillis

<400>  29

Glu Glu Leu Gln Gly Ile Ser Gly Val Gly Pro Ser Lys Ala Glu Ala 
1               5                   10                  15      


Ile Ile Ala Tyr 
            20  


<210>  30
<211>  20
<212>  PRT
<213>  Bacillus subtilis

<400>  30

Glu Asp Ile Thr Lys Val Ser Gly Ile Gly Glu Lys Ser Phe Glu Lys 
1               5                   10                  15      


Ile Lys Ser Ser 
            20  


<210>  31
<211>  20
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  31

Ser Glu Leu Thr Ala Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala 
1               5                   10                  15      


Ile Lys Asp Gly 
            20  


<210>  32
<211>  20
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  32

Arg Asp Leu Lys Glu Ile Arg Gly Ile Gly Ala Gly Arg Leu Glu Glu 
1               5                   10                  15      


Ile Lys Ala Arg 
            20  


<210>  33
<211>  50
<212>  PRT
<213>  Bacillus subtilis

<400>  33

Glu Glu Leu Gln Gly Ile Ser Gly Val Gly Pro Ser Lys Ala Glu Ala 
1               5                   10                  15      


Ile Ile Ala Tyr Arg Glu Glu Asn Gly Arg Phe Gln Thr Ile Glu Asp 
            20                  25                  30          


Ile Thr Lys Val Ser Gly Ile Gly Glu Lys Ser Phe Glu Lys Ile Lys 
        35                  40                  45              


Ser Ser 
    50  


<210>  34
<211>  46
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  34

Ser Glu Leu Thr Ala Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala 
1               5                   10                  15      


Ile Lys Asp Gly Arg Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile 
            20                  25                  30          


Arg Gly Ile Gly Ala Gly Arg Leu Glu Glu Ile Lys Ala Arg 
        35                  40                  45      


<210>  35
<211>  81
<212>  PRT
<213>  Bacillus subtilis

<400>  35

Gly Glu Glu Thr Ala Val Gln Gln Gly Gly Gly Gly Ser Val Gln Ser 
1               5                   10                  15      


Asp Gly Gly Lys Gly Ala Leu Val Asn Ile Asn Thr Ala Thr Leu Glu 
            20                  25                  30          


Glu Leu Gln Gly Ile Ser Gly Val Gly Pro Ser Lys Ala Glu Ala Ile 
        35                  40                  45              


Ile Ala Tyr Arg Glu Glu Asn Gly Arg Phe Gln Thr Ile Glu Asp Ile 
    50                  55                  60                  


Thr Lys Val Ser Gly Ile Gly Glu Lys Ser Phe Glu Lys Ile Lys Ser 
65                  70                  75                  80  


Ser 
    


<210>  36
<211>  73
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  36

Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp Gln Glu 
1               5                   10                  15      


Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val Ser Glu Leu Thr Ala 
            20                  25                  30          


Leu Pro His Ile Gly Glu Ala Arg Ala Glu Ala Ile Lys Asp Gly Arg 
        35                  40                  45              


Pro Trp Asn Ala Val Arg Asp Leu Lys Glu Ile Arg Gly Ile Gly Ala 
    50                  55                  60                  


Gly Arg Leu Glu Glu Ile Lys Ala Arg 
65                  70              


<210>  37
<211>  82
<212>  PRT
<213>  Halomonas sp. TD01

<400>  37

Thr Ala Glu Leu Leu Leu Ser Ser Phe Val Gly Gly Ala Tyr Glu Ala 
1               5                   10                  15      


Ala Asn Asp Pro Ile Tyr Cys Ile Asp Leu Asn Glu Ala Thr Ala Glu 
            20                  25                  30          


Gln Leu Thr Glu Leu Pro Asn Val Gly Pro Val Arg Ala Glu Gln Ile 
        35                  40                  45              


Ile Glu Met Arg Pro Trp Thr Asn Ala Asp Glu Leu Ser Gln Val Ser 
    50                  55                  60                  


Gly Leu Gly Val Ala Ser Val Glu Thr Ile Lys Asn Ser Gly Leu Val 
65                  70                  75                  80  


Cys Pro 
        


<210>  38
<211>  20
<212>  PRT
<213>  Halomonas sp. TD01

<400>  38

Glu Gln Leu Thr Glu Leu Pro Asn Val Gly Pro Val Arg Ala Glu Gln 
1               5                   10                  15      


Ile Ile Glu Met 
            20  


<210>  39
<211>  20
<212>  PRT
<213>  Halomonas sp. TD01

<400>  39

Asp Glu Leu Ser Gln Val Ser Gly Leu Gly Val Ala Ser Val Glu Thr 
1               5                   10                  15      


Ile Lys Asn Ser 
            20  


<210>  40
<211>  46
<212>  PRT
<213>  Halomonas sp. TD01

<400>  40

Glu Gln Leu Thr Glu Leu Pro Asn Val Gly Pro Val Arg Ala Glu Gln 
1               5                   10                  15      


Ile Ile Glu Met Arg Pro Trp Thr Asn Ala Asp Glu Leu Ser Gln Val 
            20                  25                  30          


Ser Gly Leu Gly Val Ala Ser Val Glu Thr Ile Lys Asn Ser 
        35                  40                  45      


<210>  41
<211>  77
<212>  PRT
<213>  Halomonas sp. TD01

<400>  41

Thr Ala Glu Leu Leu Leu Ser Ser Phe Val Gly Gly Ala Tyr Glu Ala 
1               5                   10                  15      


Ala Asn Asp Pro Ile Tyr Cys Ile Asp Leu Asn Glu Ala Thr Ala Glu 
            20                  25                  30          


Gln Leu Thr Glu Leu Pro Asn Val Gly Pro Val Arg Ala Glu Gln Ile 
        35                  40                  45              


Ile Glu Met Arg Pro Trp Thr Asn Ala Asp Glu Leu Ser Gln Val Ser 
    50                  55                  60                  


Gly Leu Gly Val Ala Ser Val Glu Thr Ile Lys Asn Ser 
65                  70                  75          


<210>  42
<211>  31
<212>  PRT
<213>  Bacillus subtilis

<400>  42

Gly Glu Glu Thr Ala Val Gln Gln Gly Gly Gly Gly Ser Val Gln Ser 
1               5                   10                  15      


Asp Gly Gly Lys Gly Ala Leu Val Asn Ile Asn Thr Ala Thr Leu 
            20                  25                  30      


<210>  43
<211>  27
<212>  PRT
<213>  Thioalkalivibrio sp. (strain K90mix)

<400>  43

Gly Asn Thr Leu His Asp Ala Val Val Ala Glu Ile Ala Asp Gln Glu 
1               5                   10                  15      


Ala Gly Cys Ile Asp Leu Asn Arg Ala Ser Val 
            20                  25          


<210>  44
<211>  31
<212>  PRT
<213>  Halomonas sp. TD01

<400>  44

Thr Ala Glu Leu Leu Leu Ser Ser Phe Val Gly Gly Ala Tyr Glu Ala 
1               5                   10                  15      


Ala Asn Asp Pro Ile Tyr Cys Ile Asp Leu Asn Glu Ala Thr Ala 
            20                  25                  30      


