                         SEQUENCE LISTING

<110>  Kansas State University Research Foundation
       Sun, Xiuzhi Susan
       Huang, Hongzhou
       Carter, Tiffany
 
<120>  PEPTIDE HYDROGEL PROPERTIES AND ITS APPLICATIONS

<130>  46483-PCT

<150>  US 62/008,140
<151>  2014-06-05

<160>  75    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein and a 
       trans-membrane segment of subunit IV in the dihydropyridine 
       sensitive human muscle L-type calcium channel

<400>  1

Phe Leu Ile Val Ile Gly Ser Ile Ile Gly Pro Gly Gly Asp Gly Pro 
1               5                   10                  15      


Gly Gly Asp 
            


<210>  2
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  2

Phe Leu Ile Val Ile 
1               5   


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region derived from the third trans-membrane 
       segment of subunit IV in the dihydropyridine sensitive human 
       muscle L-type calcium channel

<400>  3

Gly Ser Ile Ile 
1               


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein

<400>  4

Gly Pro Gly Gly Asp Gly Pro Gly Gly Asp 
1               5                   10  


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  5

Gly Leu Ile Val Ile 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  6

Pro Leu Ile Val Ile 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  7

Asp Leu Ile Val Ile 
1               5   


<210>  8
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  8

Val Leu Ile Val Ile 
1               5   


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  9

Ile Leu Ile Val Ile 
1               5   


<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  10

Leu Leu Ile Val Ile 
1               5   


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  11

Ala Leu Ile Val Ile 
1               5   


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  12

Phe Gly Ile Val Ile 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  13

Phe Pro Ile Val Ile 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  14

Phe Asp Ile Val Ile 
1               5   


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  15

Phe Val Ile Val Ile 
1               5   


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  16

Phe Ile Ile Val Ile 
1               5   


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  17

Phe Ala Ile Val Ile 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  18

Phe Leu Gly Val Ile 
1               5   


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  19

Phe Leu Pro Val Ile 
1               5   


<210>  20
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  20

Phe Leu Asp Val Ile 
1               5   


<210>  21
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  21

Phe Leu Val Ile Val 
1               5   


<210>  22
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  22

Phe Leu Ala Val Ile 
1               5   


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  23

Phe Leu Ile Gly Ile 
1               5   


<210>  24
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  24

Phe Leu Ile Pro Ile 
1               5   


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  25

Phe Leu Ile Asp Ile 
1               5   


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  26

Phe Leu Ile Ile Ile 
1               5   


<210>  27
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  27

Phe Leu Ile Leu Ile 
1               5   


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  28

Phe Leu Ile Ala Ile 
1               5   


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  29

Phe Leu Ile Val Gly 
1               5   


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  30

Phe Leu Ile Val Pro 
1               5   


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  31

Phe Leu Ile Val Asp 
1               5   


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  32

Phe Leu Ile Val Val 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  33

Phe Leu Ile Val Leu 
1               5   


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel

<400>  34

Phe Leu Ile Val Ala 
1               5   


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  each Xaa is individually G, A, D, R, Q, E, S, T, K, Y, H, or P

<400>  35

Gly Pro Xaa Xaa Asp 
1               5   


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  each Xaa is individually G, A, D, R, Q, E, S, T, K, Y, H, or P

<400>  36

Gly Xaa Xaa Pro Asp 
1               5   


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  each Xaa is individually G, A, D, R, Q, E, S, T, K, Y, H, or P

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  each Xaa is individually G, A, D, R, Q, E, S, T, K, Y, H, or P

<400>  37

Gly Xaa Pro Xaa Asp 
1               5   


<210>  38
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is G or A

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is G or A

<400>  38

Gly Pro Gly Xaa Asp Gly Pro Gly Xaa Asp 
1               5                   10  


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is individually A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is individually A, G, V, I, or L

<400>  39

Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, or L

<400>  40

Gly Pro Gly Xaa Asp 
1               5   


<210>  41
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region

<400>  41

Gly Gly Gly Gly 
1               


<210>  42
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  42

Gly Ser Xaa Xaa 
1               


<210>  43
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  43

Xaa Gly Ser Xaa 
1               


<210>  44
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(2)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Ser could be Thr

<400>  44

Xaa Xaa Gly Ser 
1               


<210>  45
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  45

Ser Gly Xaa Xaa 
1               


<210>  46
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  46

Xaa Ser Gly Xaa 
1               


<210>  47
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(2)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Ser could be Thr

<400>  47

Xaa Xaa Ser Gly 
1               


<210>  48
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  48

Gly Xaa Ser Xaa 
1               


<210>  49
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Ser could be Thr

<400>  49

Xaa Gly Xaa Ser 
1               


<210>  50
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)

<400>  50

Ser Xaa Gly Xaa 
1               


<210>  51
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  51

Xaa Ser Xaa Gly 
1               


<210>  52
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  each Xaa is individually G, I, V, A, L, or S

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Ser could be Thr

<400>  52

Gly Xaa Xaa Ser 
1               


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  each Xaa is individually G, I, V, A, L, or S

<400>  53

Ser Xaa Xaa Gly 
1               


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide turning region derived from the third trans-membrane 
       segment of subunit IV in the dihydropyridine sensitive human 
       muscle L-type calcium channel


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is G, I, or V

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Ser could be Thr

<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  each Xaa is individually G, I, V, A, or L

<400>  54

Xaa Ser Xaa Xaa 
1               


<210>  55
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hydrophobic region derived from the third trans-membrane segment 
       of subunit IV in the dihydropyridine sensitive human muscle 
       L-type calcium channel


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  each Xaa can be A, V, or H

<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  V and I can be switched with any amino acid in the hydrophobic 
       segment

<400>  55

Xaa Xaa Xaa Val Ile 
1               5   


<210>  56
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (1)..(4)
<223>  Any residue can be replaced by W

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa can be K, E, R, Y, or S

<220>
<221>  MISC_FEATURE
<222>  (6)..(9)
<223>  Any residue can be replaced by W

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is K, E, R, Y, or S

<400>  56

Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  57
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be K, E, R, Y, or S

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is P, A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (9)..(10)
<223>  each Xaa is K, E, R, Y, or S

<400>  57

Trp Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10      


<210>  58
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa can be K, E, R, Y, or S

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is K, E, R, Y, or S

<400>  58

Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp Trp 
1               5                   10      


<210>  59
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is A, G, V, I, H, or L

<400>  59

Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  60
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is A, G, V, I, H, or L

<400>  60

Pro Gly Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  61
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is A, G, V, I, H, or L

<400>  61

Gly Gly Pro Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  62
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is P, A, G, V, I, H, or L

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is A, G, V, I, H, or L

<400>  62

Gly Pro Gly Xaa Gly Asp Xaa Xaa Xaa Asp 
1               5                   10  


<210>  63
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is A, G, V, I, L, K, E, S, R, Y, H, or W

<400>  63

Gly Pro Gly Xaa Asp 
1               5   


<210>  64
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is K, E, R, Y, or S

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  D can be K, E, W, or S

<220>
<221>  MISC_FEATURE
<222>  (7)..(4)
<223>  Xaa is P, A, G, V, I, H, or L

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  each Xaa is K, E, R, Y, or S

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  D can be K, E, W, or S

<400>  64

Gly Pro Gly Xaa Asp Gly Xaa Xaa Xaa Asp 
1               5                   10  


<210>  65
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is G, S, Y, K, E, D, H, V, or A

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is R, Y, S, or E

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is A, G, or V

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa is D, R, S, H, V, or A

<400>  65

Gly Pro Gly Gly Asp Gly Pro Gly Gly Asp Xaa Xaa Xaa Xaa 
1               5                   10                  


<210>  66
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is G, S, Y, K, E, D, H, V, or A

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is R, Y, S, or E

<220>
<221>  MISC_FEATURE
<222>  (11)..(11)
<223>  Xaa is A, G, or V

<220>
<221>  MISC_FEATURE
<222>  (12)..(12)
<223>  Xaa is D, R, S, H, V, or A

<400>  66

Gly Pro Gly Gly Asp Gly Pro Gly Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  67
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein and a 
       trans-membrane segment of subunit IV in the dihydropyridine 
       sensitive human muscle L-type calcium channel

<400>  67

Phe Leu Ile Val Ile Gly Ser Ile Ile Pro Gly Gly Gly Asp Gly Pro 
1               5                   10                  15      


Gly Gly Asp 
            


<210>  68
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein and a 
       trans-membrane segment of subunit IV in the dihydropyridine 
       sensitive human muscle L-type calcium channel

<400>  68

Phe Leu Ile Val Ile Gly Ser Ile Ile Gly Pro Gly Val Asp Pro Gly 
1               5                   10                  15      


Gly Ala Asp 
            


<210>  69
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Derivative of spider flagelliform silk protein and a 
       trans-membrane segment of subunit IV in the dihydropyridine 
       sensitive human muscle L-type calcium channel with built-in ECM 
       ligand

<400>  69

Phe Leu Ile Val Ile Gly Ser Ile Ile Gly Pro Gly Gly Asp Gly Pro 
1               5                   10                  15      


Gly Gly Asp Gly Arg Gly Asp 
            20              


<210>  70
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  70

Gly Arg Gly Asp 
1               


<210>  71
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  71

Arg Gly Asp Ser 
1               


<210>  72
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  72

Arg Gly Asp Ser Tyr 
1               5   


<210>  73
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  73

Lys Tyr Arg Gly Asp Ser 
1               5       


<210>  74
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  74

Tyr Ile Gly Ser Arg 
1               5   


<210>  75
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic ligand

<400>  75

Ile Lys Val Ala Val 
1               5   


