               SEQUENCE LISTING

<110> ACIB GmbH - Austrian Centre of Industrial Biotechnology
      Technische Universität Graz

<120> (R)-selective nitroaldol reaction catalysed by proteins of the cupin superfamily

<130> AC007P

<150> EP14172208.2
<151> 2014-06-12

<160> 10

<170> BiSSAP 1.3

<210> 1
<211> 131
<212> PRT
<213> Granulicella tundricola

<400> 1
Met Glu Ile Lys Arg Val Gly Ser Gln Ala Ser Gly Lys Gly Pro Ala 
1               5                   10                  15      
Asp Trp Phe Thr Gly Thr Val Arg Ile Asp Pro Leu Phe Gln Ala Pro 
            20                  25                  30          
Asp Pro Ala Leu Val Ala Gly Ala Ser Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Cys Gly Trp Ala Gln Arg Glu Gly Gly Ala Val Glu Glu Ile 
65                  70                  75                  80  
His Pro Gly Asp Val Val Trp Phe Ser Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Ala Pro Thr Thr Ala Met Thr His Leu Ala Ile Gln Glu Arg 
            100                 105                 110         
Leu Asp Gly Lys Ala Val Asp Trp Met Glu His Val Thr Asp Glu Gln 
        115                 120                 125             
Tyr Arg Arg 
    130     

<210> 2
<211> 131
<212> PRT
<213> Artificial Sequence

<220> 
<223> GtHNL-A40H/V42T/Q110H

<400> 2
Met Glu Ile Lys Arg Val Gly Ser Gln Ala Ser Gly Lys Gly Pro Ala 
1               5                   10                  15      
Asp Trp Phe Thr Gly Thr Val Arg Ile Asp Pro Leu Phe Gln Ala Pro 
            20                  25                  30          
Asp Pro Ala Leu Val Ala Gly His Ser Thr Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Cys Gly Trp Ala Gln Arg Glu Gly Gly Ala Val Glu Glu Ile 
65                  70                  75                  80  
His Pro Gly Asp Val Val Trp Phe Ser Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Ala Pro Thr Thr Ala Met Thr His Leu Ala Ile His Glu Arg 
            100                 105                 110         
Leu Asp Gly Lys Ala Val Asp Trp Met Glu His Val Thr Asp Glu Gln 
        115                 120                 125             
Tyr Arg Arg 
    130     

<210> 3
<211> 131
<212> PRT
<213> Acidobacterium capsulatum

<400> 3
Met Gln Ile Thr Arg Asn Gly Ser Gln Pro Ser Gly Arg Gly Pro Ala 
1               5                   10                  15      
Glu Tyr Phe Thr Gly Thr Val Arg Val Asp Pro Leu Phe Ala Ala Pro 
            20                  25                  30          
Glu Pro Ala Arg Val Ala Gly Ala Ser Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ser Gly Cys Gly Arg Val Gln Arg Glu Gly Gly Pro Val Glu Glu Ile 
65                  70                  75                  80  
Arg Pro Gly Asp Val Val Trp Phe Thr Pro Gly Glu Arg His Trp His 
                85                  90                  95      
Gly Ala Ser Pro Ser Thr Ala Met Thr His Ile Ala Ile Gln Glu Lys 
            100                 105                 110         
Leu Asp Gly Lys Val Val Glu Trp Leu Glu His Val Thr Asp Ala Glu 
        115                 120                 125             
Tyr Ala Gly 
    130     

<210> 4
<211> 131
<212> PRT
<213> Artificial Sequence

<220> 
<223> AcHNL-A40H/V42T/Q110H

<400> 4
Met Gln Ile Thr Arg Asn Gly Ser Gln Pro Ser Gly Arg Gly Pro Ala 
1               5                   10                  15      
Glu Tyr Phe Thr Gly Thr Val Arg Val Asp Pro Leu Phe Ala Ala Pro 
            20                  25                  30          
Glu Pro Ala Arg Val Ala Gly His Ser Thr Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ser Gly Cys Gly Arg Val Gln Arg Glu Gly Gly Pro Val Glu Glu Ile 
65                  70                  75                  80  
Arg Pro Gly Asp Val Val Trp Phe Thr Pro Gly Glu Arg His Trp His 
                85                  90                  95      
Gly Ala Ser Pro Ser Thr Ala Met Thr His Ile Ala Ile His Glu Lys 
            100                 105                 110         
Leu Asp Gly Lys Val Val Glu Trp Leu Glu His Val Thr Asp Ala Glu 
        115                 120                 125             
Tyr Ala Gly 
    130     

<210> 5
<211> 131
<212> PRT
<213> Methylocella silvestris

<400> 5
Met Leu Ile Thr Arg Ser Gly Ser Gln Pro Ser Gly Lys Gly Pro Ala 
1               5                   10                  15      
Asp Trp Phe Thr Gly Ala Val Arg Met Asp Pro Leu Phe Ser Ala Pro 
            20                  25                  30          
Asp Pro Ala Arg Val Ala Gly Ala Ser Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Cys Gly Trp Ala Gln Arg Glu Gly Gly Pro Val Glu Glu Ile 
65                  70                  75                  80  
Arg Pro Gly Asp Val Ile Trp Phe Ser Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Thr Pro Thr Thr Gly Met Thr His Ile Ala Ile Gln Glu Lys 
            100                 105                 110         
Leu Asp Gly Lys Thr Val Asp Trp Leu Glu His Val Ser Asp Asp Gln 
        115                 120                 125             
Tyr Arg Met 
    130     

<210> 6
<211> 131
<212> PRT
<213> Acidiphilium cryptum

<400> 6
Met Glu Ile Trp Arg Ser Gly Ala Arg Asp Ser Thr Pro Gly Pro Gln 
1               5                   10                  15      
Ala Tyr Phe Thr Gly Ser Val Arg Ile Asp Pro Val Asn Thr Ala Pro 
            20                  25                  30          
Glu Pro Ala Arg Val Ala Ala Ala His Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ser Gly Leu Gly Trp Val Gln Arg Glu Gly Gly Pro Val Glu Glu Ile 
65                  70                  75                  80  
Arg Pro Gly Asp Val Val Trp Phe Ala Pro Gly Glu Arg His Trp His 
                85                  90                  95      
Gly Ala Thr Pro Thr Thr Gly Met Ser His Tyr Ala Ile Gln Glu Arg 
            100                 105                 110         
Leu Asp Gly Ser Ala Val Thr Trp Leu Glu His Val Thr Asp Asp Glu 
        115                 120                 125             
Tyr Arg Arg 
    130     

<210> 7
<211> 131
<212> PRT
<213> Paenibacillus sp.

<400> 7
Met Thr Ile Arg Arg Ile Gly Thr Gln Pro Ser Gly Lys Gly Pro Phe 
1               5                   10                  15      
Asp Tyr Phe Thr Gly Thr Val Arg Ile Asp Pro Leu Phe Glu Ala Pro 
            20                  25                  30          
Asp Pro Ala Arg Val Ala Gly Ala Ser Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Ser Gly Arg Ile Gln Arg Trp Gly Gly Pro Ile Glu Asp Ile 
65                  70                  75                  80  
Phe Pro Gly Asp Val Val Trp Phe Pro Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Ser Pro Thr Thr Ala Met Thr His Ile Ala Ile Gln Glu Arg 
            100                 105                 110         
Leu Asp Gly Lys Ala Val Glu Trp Leu Glu Lys Val Ser Glu Asp Gln 
        115                 120                 125             
Tyr Gln Gly 
    130     

<210> 8
<211> 135
<212> PRT
<213> Deinococcus sp.

<400> 8
Met Lys Ile Gln Arg Val Gly Thr Gln Pro Ser Thr Thr Gly Pro Ala 
1               5                   10                  15      
Asp Trp Phe Thr Gly Ala Val Arg Ile Asp Gly Leu Phe Pro Ala His 
            20                  25                  30          
Glu Pro Ala Arg Ala Ala Gly Asn Ala Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Val Gly Arg Val Gln Arg Glu Gly Gly Pro Val Glu Glu Ile 
65                  70                  75                  80  
Arg Pro Gly Asp Val Val Trp Cys Glu Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Ala Pro Thr Thr Ala Met Thr His Ile Ala Leu Gln Glu Ala 
            100                 105                 110         
Leu Asp Gly Lys Ser Val Glu Trp Leu Glu His Val Thr Asp Glu Gln 
        115                 120                 125             
Tyr Gln Ala Gly Glu Ala Gly 
    130                 135 

<210> 9
<211> 132
<212> PRT
<213> Sinorhizobium medicae

<400> 9
Met Glu Ile Phe Glu Cys Gly Ser Arg Pro Ser Thr Arg Gly Pro Ala 
1               5                   10                  15      
Glu Tyr Phe Thr Gly Ser Val Arg Leu Asp Pro Ala Phe Glu Ala Pro 
            20                  25                  30          
Ser Pro Ala Arg Leu Arg Gly Ala Thr Val Thr Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Thr Leu Ile Val Thr 
    50                  55                  60                  
Ala Gly Arg Gly Leu Ala Gln Ser Trp Gly Gly Glu Leu Arg Glu Ile 
65                  70                  75                  80  
Arg Ala Gly Asp Val Val Trp Phe Pro Pro Gly Glu Lys His Trp His 
                85                  90                  95      
Gly Ala Ala Pro Asp Thr Gly Met Thr His Ile Ala Ile Gln Glu Ala 
            100                 105                 110         
Leu Asp Gly Lys Ala Val Asp Trp Leu Glu His Val Thr Asp Glu Gln 
        115                 120                 125             
Tyr Gly Gly Val 
    130         

<210> 10
<211> 132
<212> PRT
<213> Alicyclobacillus acidocaldarius

<400> 10
Met Lys Ile Val Arg Asn Arg Glu Arg Lys Pro Ser Ala Gly Ser Ser 
1               5                   10                  15      
Ala Thr Phe Thr Gly Arg Val Ser Ile Thr Pro Val Trp Asn Ala Glu 
            20                  25                  30          
Glu Pro Ser Arg Val Gly Ala Ala Val Val Arg Phe Glu Pro Gly Ala 
        35                  40                  45              
Arg Thr Ala Trp His Thr His Pro Leu Gly Gln Leu Leu Ile Ile Leu 
    50                  55                  60                  
Glu Gly Val Gly Trp Val Gln Arg Glu Gly Glu Ser Val Gln Glu Val 
65                  70                  75                  80  
Tyr Pro Gly Asp Ile Val Trp Phe Glu Ser Gly Glu Arg His Trp His 
                85                  90                  95      
Gly Ala Ser Pro Glu His Ala Met Ala His Val Ala Ile Gln Glu Ala 
            100                 105                 110         
Leu Asp Gly Ser Pro Val Asp Trp Met Glu His Val Thr Glu Ala Glu 
        115                 120                 125             
Tyr Arg Arg Gly 
    130         

