
                                SEQUENCE LISTING

<110> BioAmber S.A.S.

<120> ORGANIC ACIDS FROM HOMOCITRATE AND
  HOMOCITRATE DERIVATIVES

<130> 39932-0005WO1

<140> PCT/US2015/035178   
<141> 2015-06-10  

<150> 62/010,371          
<151> 2014-06-10  

<160> 25

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 4910
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA 3 gene disruption fragment

<220> 
<221> misc_feature   
<222> 1656, 4312
<223> n = A,T,C or G

<400> 1
ctcaaaacta tttaattagt taattgtata aactgtatgt cattataaac agggaaggtt 60
gacattgtct agcggcaatc attgtctcat ttggttcatt aactttggtt ctgttcttgg 120
aaacgggtac caactctctc agagtgcttc aaaaattttt cagcacattt ggttagacat 180
gaactttctc tgctggttaa ggattcagag gtgaagtctt gaacacaatc gttgaaacat 240
ctgtccacaa gagatgtgta tagcctcatg aaatcagcca tttgcttttg ttcaacgatc 300
ttttgaaatt gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt 360
atgttgtagt tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt 420
gaaatgaaaa tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt 480
gcacggacat gtttttaaag gaagtactct ataccagtta ttcttcaccc tgcagggtac 540
gtagcatgca ctcgcaagct gtgccatcgc ccaacggtta attataagaa atcaacatca 600
gccaacaact attttcgtcc ccctcttttc agtggtaacg agcaattaca ttagtaagag 660
actattttct tcagtgattt gtaatttttt ttcagtgatt tgtaattctt tctcgaaata 720
tgcgggctta acttatccgg acattcacta catgcaagga aaaacgagaa ccgcggagat 780
ttcctcagta agtaacaatg atgatctttt tacgcttcat catcactttc caaagttcta 840
agctataagt tcaagcctag atacgctgaa aaactcctga ccaacaatgt aaagaaaaca 900
attacaattg taaggttgaa aacatctaaa aatgaaatat tttattgtac atgcacaccc 960
tgatagtcat tctcttactt catccctgaa agacgtggct gtacaagagt tggaatcgca 1020
aggtcatgag gttaaagtta gtgatcttta tgctcaaaag tggaaggcct caatagaccg 1080
tgacgacttc gagcagcttt tcgcaagaag agaggttaaa aataccccaa gcttcttatg 1140
aagcgtatgc cagaggagca ttaacaaaag acgtaaatca ggaacaggaa aaacttattt 1200
gggcggactt tgtcattttg tcgtttccta tatggtggtc ttctatgccg gctagtcgac 1260
cccctcgacc ccctcgagcg atctcgagat ttgctgcaac ggcaacatca atgtccacgt 1320
ttacacacct acatttatat ctatatttat atttatattt atttatttat gctacttagc 1380
ttctatagtt agttaatgca ctcacgatat tcaaaattga cacccttcaa ctactcccta 1440
ctattgtcta ctactgtcta ctactcctct ttactatagc tgctcccaat aggctccacc 1500
aataggctct gtcaatacat tttgcgccgc cacctttcag gttgtgtcac tcctgaagga 1560
ccatattggg taatcgtgca atttctggaa gagagtgccg cgagaagtga ggcccccact 1620
gtaaatcctc gagggggcat ggagtatggg gcatgnagga tggaggatgg gggggggggg 1680
ggaaaatagg tagcgaaagg acccgctatc accccacccg gagaactcgt tgccgggaag 1740
tcatatttcg acactccggg gagtctataa aaggcgggtt ttgtcttttg ccagttgatg 1800
ttgctgagag gacttgtttg ccgtttcttc cgatttaaca gtatagaatc aaccactgtt 1860
aattatacac gttatactaa cacaacaaaa acaaaaacaa cgacaacaac aacaacaatg 1920
tttgctttct actttctcac cgcatgcacc actttgaagg gtgttttcgg agtttctccg 1980
agttacaatg gtcttggtct caccccacag atgggttggg acagctggaa tacgtttgcc 2040
tgcgatgtca gtgaacagct acttctagac actgctgata gaatttctga cttggggcta 2100
aaggatatgg gttacaagta tgtcatccta gatgactgtt ggtctagcgg cagggattcc 2160
gacggtttcc tcgttgcaga caagcacaaa tttcccaacg gtatgggcca tgttgcagac 2220
cacctgcata ataacagctt tcttttcggt atgtattcgt ctgctggtga gtacacctgt 2280
gctgggtacc ctgggtctct ggggcgtgag gaagaagatg ctcaattctt tgcaaataac 2340
cgcgttgact acttgaagta tgataattgt tacaataaag gtcaatttgg tacaccagac 2400
gtttcttacc accgttacaa ggccatgtca gatgctttga ataaaactgg taggcctatt 2460
ttctattctc tatgtaactg gggtcaggat ttgacatttt actggggctc tggtatcgcc 2520
aattcttgga gaatgagcgg agatattact gctgagttca cccgtccaga tagcagatgt 2580
ccctgtgacg gtgacgaata tgattgcaag tacgccggtt tccattgttc tattatgaat 2640
attcttaaca aggcagctcc aatggggcaa aatgcaggtg ttggtggttg gaacgatctg 2700
gacaatctag aggtcggagt cggtaatttg actgacgatg aggaaaaggc ccatttctct 2760
atgtgggcaa tggtaaagtc cccacttatc attggtgccg acgtgaatca cttaaaggca 2820
tcttcgtact cgatctacag tcaagcctct gtcatcgcaa ttaatcaaga tccaaagggt 2880
attccagcca caagagtctg gagatattat gtttcagaca ccgatgaata tggacaaggt 2940
gaaattcaaa tgtggagtgg tccgcttgac aatggtgacc aagtggttgc tttattgaat 3000
ggaggaagcg tagcaagacc aatgaacacg accttggaag agattttctt tgacagcaat 3060
ttgggttcaa aggaactgac atcgacttgg gatatttacg acttatgggc caacagagtt 3120
gacaactcta cggcgtctgc tatccttgaa cagaataagg cagccaccgg tattctctac 3180
aatgctacag agcagtctta taaagacggt ttgtctaaga atgatacaag actgtttggc 3240
cagaaaattg gtagtctttc tccaaatgct atacttaaca caactgttcc agctcatggt 3300
atcgccttct ataggttgag accctcggct taagctcaat gttgagcaaa gcaggacgag 3360
aaaaaaaaaa ataatgattg ttaagaagtt catgaaaaaa aaaaggaaaa atactcaaat 3420
acttataaca gagtgattaa ataataaacg gcagtatacc ctatcaggta ttgagatagt 3480
tttatttttg taggtatata atctgaagcc tttgaactat tttctcgtat atatcatgga 3540
gtatacattg cattagcaac attgcatact agtcactcgc aagctgtgcc atcgcccaac 3600
ggttaattat aagaaatcaa catcagccaa caactatttt cgtccccctc ttttcagtgg 3660
taacgagcaa ttacattagt aagagactat tttcttcagt gatttgtaat tttttttcag 3720
tgatttgtaa ttctttctcg aaatatgcgg gctwaamtaa tccggacatt cactacatgc 3780
aaggaaaaac gagaaccgcg gagatttcct cagtaagtaa caatgatgat ctttttacgc 3840
ttcatcatca ctttccaaag ttctaagcta taagttcaag cctagatacg ctgaaaaact 3900
cctgaccaac aatgtaaaga aaacaattac aattgtaagg ttgaaaacat ctaaaaatga 3960
aatattttat tgtacatgca caccctgata gtcattctct tacttcatcc ctgaaagacg 4020
tggctgtaca agagttggaa tcgcaaggtc atgaggttaa agttagtgat ctttatgctc 4080
aaaagtggaa ggcctcaata gaccgtgacg acwwmaaaaa amaaamrmaa gaagagaggt 4140
taaaaatacc ccaagcttct tatgaagcgt atgccagagg agcattaaca aaagacgtaa 4200
atcaggaaca ggaaaaactt atttgggcgg actttgtcat tttgtcgttt cctatatggt 4260
ggtcttctat gccggctagc ggccgggcaa caaagcctcc cagatttgat anattttcaa 4320
tttgtgcttt gaatcatgac ttccacctgt ttggtccgca agaacacgta aatgcgcaat 4380
ttgtttctcc cttctgctta aaaaccatgc acctttaata ttatctggaa agataaagaa 4440
cagaattgtt gcgtagaaac aagtagcaga gccgtaaatg agaaaaatat acttccaagc 4500
tggtaatttc ccctttatta gtccaataca gtgtccgaag accccaccaa gaataccagc 4560
aagggtgttg aaatataatg tagatcttag tggttgttct gatttcttcc accacattcc 4620
gctaataatc ataaaagacg gtaatattcc ggcttcaaat acgccaagaa aaaacctcac 4680
ggtaaccaaa ccaccaaagc tatgacatgc agccatgcac ataagtaagc cgccccaaat 4740
gaacaaacaa atagacacaa atttgccaat tctaactcgt ggcaacaaaa aaaaggatat 4800
gaactcacct aataaataac cgaaataaaa agtagaagca actgtggaaa attgagaacc 4860
atgtaaattt gtgtcttctt tcaatgtata aacagccgca atacctaggg            4910

<210> 2
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 2
gcaactgatg ttcacgaatg cg                                          22

<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 3
ttgccgttgc agcaaatctc                                             20

<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 4
acggcagtat accctatcag g                                           21

<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 5
aatgatccat ggtccgagag                                             20

<210> 6
<211> 3704
<212> DNA
<213> Artificial Sequence

<220> 
<223> PDC gene disruption fragment

<400> 6
cccccagttg ttgttgcaat taacaaattt gctaccgaca ccgagaagga aattgagacc 60
attagagaag aagccatcaa ggctggtgca tttgatgctg ttgagtcaga ccattggtca 120
caaggtggta agggtgcaat caagttagct gaggcaattg tacgtgctac cgaggaaaga 180
ccgttggaag aaagtcaacc tcctaactat ctttattcat tagatggttc gttagaagat 240
agactaagaa caattgccac caagatgtat ggagcaaaag atattgaact atctgagttg 300
gccaagaaac agattgaaga gtatgagagt caaggttttg gcaagctgcc tgtttgtatt 360
gcaaagacgc aatattctct ctcccatgat ccaacattga aaggtgttcc aaaggatttc 420
atcttcccaa tcagagaagt tagaataagt gcaggagcag gatatttata tgcactagct 480
gcaaagatca tgacaattcc aggtctatca acttatgccg gatttatgaa tgttgaagtc 540
aacgaagatg gtgagattga tggattgttt tagtttttat tataaaatta tatattattc 600
ttaattacat atcacccttc tatcagggaa gggagaaacg aaaatagaga gtgacctatc 660
caagctcggg ggtctaagtt ttaatggccc agggaatcat tacttttttt tctcaatcct 720
tgatggataa aagtattaca tacgtacagg attgtgtatt agtgtatttc gttatatgat 780
taaacaaagt ttatagattg taaagtagac gtaaagttta gtaattcatt ttaatgttca 840
ttttacattc agatggcggc cgcggatcca gatcccccgg ggcgttgaag atctattctc 900
cagcaattaa atttgtgaag aataactggt atagagtact tcctttaaaa acatgtccgt 960
gcaccaagaa aaaaaaaaag tttgaaaaat tgtatgtcga cgaatttcag cattttcatt 1020
tcaaggcgat attatgtttc actaaactca ggacaggaat atactaagaa taactacaac 1080
atacacacaa cataagccaa gatggatcaa cttaactacc aagaacaaca acaatttcaa 1140
aagatcgttg aacaaaagca aatggctgat ttcatgaggc tatctgcaga tacgcggaac 1200
aatcaatcga taatgatttg actgataaag aaaaccatac ttttgtttat gtttattagt 1260
tatcgctttg ctacattaaa aattcacata ctaaagcctt tgttaaacaa ctttttctaa 1320
atcttaagat tttactctat ctagtttttt tggttgtagg tgaacgtaaa gtacctcatt 1380
tatttttttt tttttgcttg tgtaattctt ttcatgctta tttaaactag tgtacatgta 1440
tcaaatcttt gtgtaagaat catttaaatc tgtttaaata agcattccaa ccagcttgtt 1500
ggtatctttt agcttgctct ataggatctc ttccttgacc gtacaaacct ctaccaacaa 1560
ttatgatatc cgttccagtc tttacaactt catcaacagt tctatattgt tgaccaagtg 1620
catcaccttt gtcatctaaa ccaacccctg gagtcataat gatccagtca aaaccttctt 1680
ctctaccgcc catatcgtgt tgcgcaataa aaccaatgac aaactcttta tcagatttag 1740
caatttctac tgttttttct gtatattcac catatgctaa agaacccttt gatgataact 1800
cagcaagcat tagcaaacct ctaggttcac tggttgtttc ttgggctgcc tccttcaagc 1860
cagaaacaat acctgcaccc gttacaccat gtgcattagt gatgtcagcc cattcggcaa 1920
tacggaagac accagattta tattgatttt taacagtgtt accaatatca gcaaattttc 1980
tatcttcaaa aatcataaaa ttatgtttct tggcaagctc cttcaaaggc aacacagttc 2040
cttcatacgt aaaatcagaa acaatatcga tgtgtgtttt aactagacag atgtaaggac 2100
caatagtgtc caaaatagag agaagctttt cagtttcagt aatatccaat gatgcacaaa 2160
ggttagactt cttttcctcc atgatggaga aaagtctcct agcaacaggg gaagtgtgtg 2220
attctgatct ttctttgtat gacgccatcc ttgacaaaca aactacttta ttaaagcgtt 2280
gaagatctat tctccagcaa ttaaatttgt gaagaataac tggtatagag tacttccttt 2340
aaaaacatgt ccgtgcacca agaaaaaaaa aaagtttgaa aaattgtatg tcgacgaatt 2400
tcagcatttt catttcaagg cgatattatg tttcactaaa ctcaggacag gaatatacta 2460
agaataacta caacatacac acaacataag ccaagatgga tcaacttaac taccaagaac 2520
aacaacaatt tcaaaagatc gttgaacaaa agcaaatggc tgatttcatg aggctatgaa 2580
ttcttttatt ataaaattat atattattct taattacata tcacccttct atcagggaag 2640
ggagaaacga aaatagagag tgacctatcc aagcttgggg gtctaagttt taatggccca 2700
gggaatcatt actttttttt ctcaatcctt gatggataaa agtattacat acgtacagga 2760
ttgtgtatta gtgtatttcg ttatatgatt aaacaaagtt tatagattgt aaagtagacg 2820
taaagtttag taattcattt taatgttcat tttacattca gatgttaatt aaggcctcga 2880
gggatccgcg gccgctattt ttgtgttttg ctgtgttttg ttttattttg ttttattggg 2940
aagaaaatat ataataatag aatattatat taacaaataa ttaaagaagc tcaactgtta 3000
ttagaataaa tgggttctcc gtgtcctttt tatacgcctt ctccgaaaag aaaaaaacca 3060
tcgtatcatt tgtagcccac gccacccgga aaaaccacca ttgtcctcag cagtccgcaa 3120
aaatatggat gcgctcaatc aatttccctc ccccgtcaat gccaaaagga taacgacaca 3180
ctattaagag cgcatcattt gtaaaagccg aggaaggggg atacgctgac cgagacgtct 3240
cgcctcactc tcggagctga gccgccctcc ttaagaaatt catgggaaga acacccttcg 3300
cggcttctga acggctcgcc ctcgtccatt ggtcacctca cagtggcaac taataaggac 3360
attatagcaa tagaaattaa aatggtgcac agaaatacaa taggatcgaa taggatagga 3420
tacaataaga tacggaatat tagactatac tgtgatacgg tacgctacga tacgctacga 3480
tacgatacga tagaggatac cacggatata acgtagtgtt atttttcatt attggggttt 3540
tttttctgtt tgaattttcc acgtcaagag tatcccatct gacaggaacc gatggactcg 3600
tcacagtacc tatcgcccga gttcaatcca tggacgctgc gggtgaagga tcttcgcccg 3660
ctgttggcaa gccatgggat cagggcgtcg ccaagggacg ggcc                  3704

<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 7
gaagagacgt acaagatccg cc                                          22

<210> 8
<211> 47
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 8
ggataaaagt attacatacg tacaggattg tgtattagtg tatttcg               47

<210> 9
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 9
taggaatggt gcatcatcca ac                                          22

<210> 10
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 10
gctggagaat agatcttcaa cgccccg                                     27

<210> 11
<211> 4106
<212> DNA
<213> Artificial Sequence

<220> 
<223> LYS4 D123N gene integration fragment

<400> 11
ctaagtagtg gtgttggtga actcaagatg gactctttag gtaattatat tcttgaatag 60
ttgtgtaaag cgaatatgca aatagatttg ttttataatt atgcatctct ttgaaagagg 120
tttagaggca aagttcttgc atacaatatt gtgattgttt taatgtcatt cttgattttc 180
ataaagagat taaaaaaaaa aaaaaaaaac ttataaaatt gagtagaacc atttatatat 240
aagacaaaga ttgtctgtat tagtcctcaa cacactaaac cttacatact tagggtaaat 300
ttgctaatag agtgatatgt tcatgagaac tccaacgaca acacaaccac ctatttgcac 360
aacaaacacc attgtcgcac gctgcgcgcc ctagaagtag aaagaaaggg aaatgacatt 420
aagagaatca taccccgtgc ccgtaacgcc gaaaaaatca caccccgtcc cccacacctt 480
aaaacctcaa ccgcttaaca ccgccacacc ctttctcttt ataaacgccg tttgcattac 540
tcattcttct tataaaccgc accccccaaa acgcggaata gcttcaaccc cccaatcaga 600
tatgagtttc ccgggaaacc cgcttttccc gacagcccca caaggggttg gtctataaaa 660
gaggacgttt tccccgtcat cgagattgaa gattcttaca ggcccattta ttcaaattgg 720
agttgattct tcttgtcttt actttctttc tctctttttc ttcctttttt aatattatct 780
tttgtcaagc ctggttccct aagttgaact ctcttttctt gtgatcctcc tatatagata 840
cgccttgcca aatgcggccg cgagtccatc ggttcctgtc agatgggata ctcttgacgt 900
ggaaaattca aacagaaaaa aaaccccaat aatgaaaaat aacactacgt tatatccgtg 960
gtatcctcta tcgtatcgta tcgtagcgta tcgtagcgta ccgtatcaca gtatagtcta 1020
atattccgta tcttattgta tcctatccta ttcgatccta ttgtatttca gtgcaccatt 1080
ttaatttcta ttgctataat gtccttatta gttgccactg tgaggtgacc aatggacgag 1140
ggcgagccgt tcagaagccg cgaagggtgt tcttcccatg aatttcttaa ggagggcggc 1200
tcagctccga gagtgaggcg agacgtctcg gtcagcgtat cccccttcct cggcttttac 1260
aaatgatgcg ctcttaatag tgtgtcgtta tccttttggc attgacgggg gagggaaatt 1320
gattgagcgc atccatattt ttgcggactg ctgaggacaa tggtggtttt tccgggtggc 1380
gtgggctaca aatgatacga tggttttttt cttttcggag aaggcgtata aaaaggacac 1440
ggagaaccca tttattctaa aaacagttga gcttctttaa ttattttttg atataatatt 1500
ctattattat atattttctt cccaataaaa caaaataaaa caaaacacag caaaacacaa 1560
aaattctaga taaaatgagt gtctctgagg caaacggaac cgaaactatc aaacctccaa 1620
tgaacggaaa cccatacgga cctaatccat ctgacttctt gtccagagtt aacaacttct 1680
caatcatcga aagtaccttg agagaaggag agcaattcgc taacgccttt ttcgatactg 1740
agaagaaaat tcagatcgca aaggctcttg ataacttcgg agttgactac atcgaattga 1800
cttccccagt tgcttcagaa caaagtagac aggactgtga ggctatctgc aaattgggtc 1860
ttaagtgtaa aattttgact catatcagat gccacatgga tgacgctaga gttgccgtcg 1920
aaactggtgt tgatggagtc aacgttgtca ttggtacatc tcaatacttg agaaagtact 1980
ctcatggtaa agatatgacc tacatcatcg actctgctac tgaggttatt aattttgtca 2040
agtccaaagg tatcgaagtt agattttctt ccgaggattc tttcagatcc gatcttgttg 2100
acttgctttc tttgtacaag gctgttgata aaattggtgt taacagagtc ggaatcgcag 2160
acaccgttgg ttgtgctact cctagacaag tctacgatct tatcagaaca ttgagaggag 2220
ttgtctcctg tgatattgaa tgccatttcc acaacgacac cggtatggcc attgcaaatg 2280
cttactgcgc tttggaagcc ggagcaactc atatcgatac atcaatcttg ggtatcggag 2340
agagaaatgg tatcactcca ttgggagcat tgcttgctag aatgtacgtt acagatagag 2400
aatacatcac ccacaagtac aaacttaacc aattgagaga acttgagaat ttggttgctg 2460
acgccgttga ggtccagatt ccttttaaca attacatcac tggaatgtgt gccttcacac 2520
ataaggcagg tattcatgca aaagctatcc ttgctaaccc atcaacttac gaaattttga 2580
agcctgagga tttcggaatg tcaagatatg ttcatgtcgg tagtagattg acaggatgga 2640
acgctattaa gagtagagcc gaacaattga atcttcactt gacagacgcc caggcaaaag 2700
agttgaccgt tagaattaag aaacttgccg atgtcagaac tttggctatg gatgacgttg 2760
acagagtctt gagagaatac cacgcagact tgtccgacgc agatagaatc actaaggaag 2820
cctccgccta attaattctg tctttgattt tcttatgtta ttcaaaacat ctgccccaaa 2880
atctaacgat tatatatatt cctacgtata actgtatagc taattattga tttatttgta 2940
cataaaaacc acataaatgt aaaagcaaga aaaaaaataa ctaaggagaa ggatcaatat 3000
ctcatttata atgctcgcca aagcagcgta cgtgaatttt aatcaagaca tcaacaaatc 3060
ttgcaacttg gttatatcgc ttcttcaccc actcacccgc ttttctacat tgttgaacac 3120
aaatatatac aggggtatgt ctcaaggtca agtgcagttt caacagagac tacctcaagg 3180
tacctcttca gaaatgcaga acttcactct tgatcagatt ttctccgaat taaaggttta 3240
aacatagcct catgaaatca gccatttgct tttgttcaac gatcttttga aattgttgtt 3300
gttcttggta gttaagttga tccatcttgg cttatgttgt gtgtatgttg tagttattct 3360
tagtatattc ctgtcctgag tttagtgaaa cataatatcg ccttgaaatg aaaatgctga 3420
aattcgtcga catacaattt ttcaaacttt ttttttttct tggtgcacgg acatgttttt 3480
aaaggaagta ctctatacca gttattcttc acaaatttaa ttgctggaga atagatcttc 3540
aacgctttaa taaagtagtt tgtttgtcaa ggatggcgtc atacaaagaa agatcagaat 3600
cacacacttc ccctgttgct aggagacttt tctccatcat ggaggaaaag aagtctaacc 3660
tttgtgcatc attggatatt actgaaactg aaaagcttct ctctattttg gacactattg 3720
gtccttacat ctgtctagtt aaaacacaca tcgatattgt ttctgatttt acgtatgaag 3780
gaactgtgtt gcctttgaag gagcttgcca agaaacataa ttttatgatt tttgaagata 3840
gaaaatttgc tgatattggt aacactgtta aaaatcaata taaatctggt gtcttccgta 3900
ttgccgaatg ggctgacatc actaatgcac atggtgtaac gggtgcaggt attgtttctg 3960
gcttgaagga ggcagcccaa gaaacaacca gtgaacctag aggtttgcta atgcttgctg 4020
agttatcatc aaagggttct ttagcatatg gtgaatatac agaaaaaaca gtagaaattg 4080
ctaaatctga taaagagttt gttgag                                      4106

<210> 12
<211> 1257
<212> DNA
<213> Artificial Sequence

<220> 
<223> codon optimized S Pombe LYS4 D123N

<400> 12
atgagtgtct ctgaggcaaa cggaaccgaa actatcaaac ctccaatgaa cggaaaccca 60
tacggaccta atccatctga cttcttgtcc agagttaaca acttctcaat catcgaaagt 120
accttgagag aaggagagca attcgctaac gcctttttcg atactgagaa gaaaattcag 180
atcgcaaagg ctcttgataa cttcggagtt gactacatcg aattgacttc cccagttgct 240
tcagaacaaa gtagacagga ctgtgaggct atctgcaaat tgggtcttaa gtgtaaaatt 300
ttgactcata tcagatgcca catggatgac gctagagttg ccgtcgaaac tggtgttgat 360
ggagtcaacg ttgtcattgg tacatctcaa tacttgagaa agtactctca tggtaaagat 420
atgacctaca tcatcgactc tgctactgag gttattaatt ttgtcaagtc caaaggtatc 480
gaagttagat tttcttccga ggattctttc agatccgatc ttgttgactt gctttctttg 540
tacaaggctg ttgataaaat tggtgttaac agagtcggaa tcgcagacac cgttggttgt 600
gctactccta gacaagtcta cgatcttatc agaacattga gaggagttgt ctcctgtgat 660
attgaatgcc atttccacaa cgacaccggt atggccattg caaatgctta ctgcgctttg 720
gaagccggag caactcatat cgatacatca atcttgggta tcggagagag aaatggtatc 780
actccattgg gagcattgct tgctagaatg tacgttacag atagagaata catcacccac 840
aagtacaaac ttaaccaatt gagagaactt gagaatttgg ttgctgacgc cgttgaggtc 900
cagattcctt ttaacaatta catcactgga atgtgtgcct tcacacataa ggcaggtatt 960
catgcaaaag ctatccttgc taacccatca acttacgaaa ttttgaagcc tgaggatttc 1020
ggaatgtcaa gatatgttca tgtcggtagt agattgacag gatggaacgc tattaagagt 1080
agagccgaac aattgaatct tcacttgaca gacgcccagg caaaagagtt gaccgttaga 1140
attaagaaac ttgccgatgt cagaactttg gctatggatg acgttgacag agtcttgaga 1200
gaataccacg cagacttgtc cgacgcagat agaatcacta aggaagcctc cgcctaa    1257

<210> 13
<211> 2592
<212> DNA
<213> Artificial Sequence

<220> 
<223> C-term URA3  gene integration fragment

<400> 13
ctttgaagga gcttgccaag aaacataatt ttatgatttt tgaagataga aaatttgctg 60
atattggtaa cactgttaaa aatcaatata aatctggtgt cttccgtatt gccgaatggg 120
ctgacatcac taatgcacat ggtgtaacgg gtgcaggtat tgtttctggc ttgaaggagg 180
cagcccaaga aacaaccagt gaacctagag gtttgctaat gcttgctgag ttatcatcaa 240
agggttcttt agcatatggt gaatatacag aaaaaacagt agaaattgct aaatctgata 300
aagagtttgt cattggtttt attgcgcaac acgatatggg cggtagagaa gaaggttttg 360
actggatcat tatgactcca ggggttggtt tagatgacaa aggtgatgca cttggtcaac 420
aatatagaac tgttgatgaa gttgtaaaga ctggaacgga tatcataatt gttggtagag 480
gtttgtacgg tcaaggaaga gatcctatag agcaagctaa aagataccaa caagctggtt 540
ggaatgctta tttaaacaga tttaaatgat tcttacacaa agatttgata catgtacact 600
agtttaaata agcatgaaaa gaattacaca agcaaaaaaa aaaaaataaa tgaggtactt 660
tacgttcacc tacaaccaaa aaaactagat agagtaaaat cttaagattt agaaaaagtt 720
gtttaacaaa ggctttagta tgtgaatttt taatgtagca aagcgataac taataaacat 780
aaacaaaagt atggttttct ttatcagtca aatcattatc gattgattgt tccgcgtatc 840
tgcagatagc ctcatgaaat cagccatttg cttttgttca acgatctttt gaaattgttg 900
ttgttcttgg tagttaagtt gatccatctt ggcttatgtt gtgtgtatgt tgtagttatt 960
cttagtatat tcctgtcctg agtttagtga aacataatat cgccttgaaa tgaaaatgct 1020
gaaattcgtc gacatacaat ttttcaaact tttttttttt cttggtgcac ggacatgttt 1080
ttaaaggaag tactctatac cagttattct tcacaaattt aattgctgga gaatagatct 1140
tcaacgcgtt tggcgcgcca tctaatagtt taatcacagc ttatagtcta ctatagtttt 1200
cttttttaaa cattgttgta ttttgtcccc cccctctaat tgatgatgat tatcctataa 1260
gaatccaata aaacgatgga aactaatacc ctctcctttg tcatgtggtc tttagtattt 1320
cttgaacatt ggctctgatt tctcgacttt atagtcctat taaaatcgct gttagttctc 1380
gatcgttgta tctcgtttct tgtctctttg gtggatgatt ttgcgtgcga acatgttttt 1440
ttccctttct ctcaccatca tcgtgtagtt cttgtcacca tcccccccac cccttccttc 1500
tctcattgat tctataagag cttatccaca gaggtgcagt aacgaggtag tttaaccttc 1560
gagtggatca aaatgtcaca caggcctgcg gccgctacca taatgtatgc gttgagcctc 1620
ttgcaccttc tttattagga aatcagttga aaaatttccg gattgtcttt attattggcc 1680
catttttttt tggtcacacc tttatttttg tacacttctc gggcaaagca aaaactatag 1740
taccggatag gcctttataa aactccagtg tgtatgattt tagttggtgt gccatctaca 1800
cgttctctta gtttctttat catgtcacag aaagcaagca tgcaaaccct tacaaaaaat 1860
aacaacatac aaatgcctaa acaactggac tataatgatg gtgagtcagt tacgaaaaga 1920
gcaagtgggt taatacgatt tcgtaaggga cagtctgagg aagactacaa ttttcaaaag 1980
gagcagttct ggtccacggg tcctttagta cagaatcaca catttgtgac tgaatttgtt 2040
gaaaagttta ttgaaaacac aattagtgaa gattattcaa tcacagatag atcgaaaata 2100
gaacgtgaaa caatcataca cggattggag aagctgtatt ttcaaaggga atatgagcga 2160
tgtctaaaag atgttcaact attgaaggac aatatcgata agttcaatcc taatttggat 2220
cttaatgaaa agaatttata atgagctgaa ttatatttct tggatgtgca tcaaaaagat 2280
ccatgagagt aacgaaaaga aactggggga aatctaataa tttacaattt caatatacac 2340
ttctatatcc tttaatgtaa tggctttata aataaacacg aacttctaca gcaccgacgt 2400
ttctttttct taccagctcc tcttcttctt cttcttcttc ttcttcttct tcttcttctt 2460
cttcttcttc ttcttcttct tcttctttct taccatcatt gccattttcc ttttttctta 2520
tttgctcttg atcctctgtt ttttcaattt ggacaaactc atctaataca ccaacacttt 2580
tagggccccc gc                                                     2592

<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 14
ggaggaatgg aacagtgatg ac                                          22

<210> 15
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 15
caagagtatc ccatctgaca ggaaccgatg g                                31

<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 16
cacagaggtg cagtaacgag                                             20

<210> 17
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 17
catcactgtt aaaggaatgg gtaaatc                                     27

<210> 18
<211> 4241
<212> DNA
<213> Artificial Sequence

<220> 
<223> LYS4 D123N gene inserter fragment

<400> 18
ctaaaagtgt tggtgtatta gatgagtttg tccaaattga aaaaacagag gatcaagagc 60
aaataagaaa aaaggaaaat ggcaatgatg gtaagaaaga agaagaagaa gaagaagaag 120
aagaagaaga agaagaagaa gaagaagaag aagaagaaga agaggagctg gtaagaaaaa 180
gaaacgtcgg tgctgtagaa gttcgtgttt atttataaag ccattacatt aaaggatata 240
gaagtgtata ttgaaattgt aaattattag atttccccca gtttcttttc gttactctca 300
tggatctttt tgatgcacat ccaagaaata taattcagct cattataaat tcttttcatt 360
aagatccaaa ttaggattga acttatcgat attgtccttc aatagttgaa catcttttag 420
acatcgctca tattcccttt gaaaatacag cttctccaat ccgtgtatga ttgtttcacg 480
ttctattttc gatctatctg tgattgaata atcttcacta attgtgtttt caataaactt 540
ttcaacaaat tcagtcacaa atgtgtgatt ctgtactaaa ggacccgtgg accagaactg 600
ctccttttga aaattgtagt cttcctcaga ctgtccctta cgaaatcgta ttaacccact 660
tgctcttttc gtaactgact caccatcatt atagtccagt tgtttaggca tttgtatgtt 720
gttatttttt gtaagggttt gcatgcttgc tttctgtgac atgataaaga aactaagaga 780
acgtgtagat ggcacaccaa ctaaaatcat acacactgga gttttataaa ggcctatccg 840
gtactatagt ttttgctttg cccgagaagt gtacaaaaat aaaggtgtga ccaaaaaaaa 900
atgggccaat aataaagaca atccggaaat ttttcaactg atttcctaat aaagaaggtg 960
caagaggctc aacgcataca ttatggtagc ggccgcgagt ccatcggttc ctgtcagatg 1020
ggatactctt gacgtggaaa attcaaacag aaaaaaaacc ccaataatga aaaataacac 1080
tacgttatat ccgtggtatc ctctatcgta tcgtatcgta gcgtatcgta gcgtaccgta 1140
tcacagtata gtctaatatt ccgtatctta ttgtatccta tcctattcga tcctattgta 1200
tttcagtgca ccattttaat ttctattgct ataatgtcct tattagttgc cactgtgagg 1260
tgaccaatgg acgagggcga gccgttcaga agccgcgaag ggtgttcttc ccatgaattt 1320
cttaaggagg gcggctcagc tccgagagtg aggcgagacg tctcggtcag cgtatccccc 1380
ttcctcggct tttacaaatg atgcgctctt aatagtgtgt cgttatcctt ttggcattga 1440
cgggggaggg aaattgattg agcgcatcca tatttttgcg gactgctgag gacaatggtg 1500
gtttttccgg gtggcgtggg ctacaaatga tacgatggtt tttttctttt cggagaaggc 1560
gtataaaaag gacacggaga acccatttat tctaaaaaca gttgagcttc tttaattatt 1620
ttttgatata atattctatt attatatatt ttcttcccaa taaaacaaaa taaaacaaaa 1680
cacagcaaaa cacaaaaatt ctagataaaa tgagtgtctc tgaggcaaac ggaaccgaaa 1740
ctatcaaacc tccaatgaac ggaaacccat acggacctaa tccatctgac ttcttgtcca 1800
gagttaacaa cttctcaatc atcgaaagta ccttgagaga aggagagcaa ttcgctaacg 1860
cctttttcga tactgagaag aaaattcaga tcgcaaaggc tcttgataac ttcggagttg 1920
actacatcga attgacttcc ccagttgctt cagaacaaag tagacaggac tgtgaggcta 1980
tctgcaaatt gggtcttaag tgtaaaattt tgactcatat cagatgccac atggatgacg 2040
ctagagttgc cgtcgaaact ggtgttgatg gagtcaacgt tgtcattggt acatctcaat 2100
acttgagaaa gtactctcat ggtaaagata tgacctacat catcgactct gctactgagg 2160
ttattaattt tgtcaagtcc aaaggtatcg aagttagatt ttcttccgag gattctttca 2220
gatccgatct tgttgacttg ctttctttgt acaaggctgt tgataaaatt ggtgttaaca 2280
gagtcggaat cgcagacacc gttggttgtg ctactcctag acaagtctac gatcttatca 2340
gaacattgag aggagttgtc tcctgtgata ttgaatgcca tttccacaac gacaccggta 2400
tggccattgc aaatgcttac tgcgctttgg aagccggagc aactcatatc gatacatcaa 2460
tcttgggtat cggagagaga aatggtatca ctccattggg agcattgctt gctagaatgt 2520
acgttacaga tagagaatac atcacccaca agtacaaact taaccaattg agagaacttg 2580
agaatttggt tgctgacgcc gttgaggtcc agattccttt taacaattac atcactggaa 2640
tgtgtgcctt cacacataag gcaggtattc atgcaaaagc tatccttgct aacccatcaa 2700
cttacgaaat tttgaagcct gaggatttcg gaatgtcaag atatgttcat gtcggtagta 2760
gattgacagg atggaacgct attaagagta gagccgaaca attgaatctt cacttgacag 2820
acgcccaggc aaaagagttg accgttagaa ttaagaaact tgccgatgtc agaactttgg 2880
ctatggatga cgttgacaga gtcttgagag aataccacgc agacttgtcc gacgcagata 2940
gaatcactaa ggaagcctcc gcctaattaa ttctgtcttt gattttctta tgttattcaa 3000
aacatctgcc ccaaaatcta acgattatat atattcctac gtataactgt atagctaatt 3060
attgatttat ttgtacataa aaaccacata aatgtaaaag caagaaaaaa aataactaag 3120
gagaaggatc aatatctcat ttataatgct cgccaaagca gcgtacgtga attttaatca 3180
agacatcaac aaatcttgca acttggttat atcgcttctt cacccactca cccgcttttc 3240
tacattgttg aacacaaata tatacagggg tatgtctcaa ggtcaagtgc agtttcaaca 3300
gagactacct caaggtacct cttcagaaat gcagaacttc actcttgatc agattttctc 3360
cgaattaaag gtttaaacat agcctcatga aatcagccat ttgcttttgt tcaacgatct 3420
tttgaaattg ttgttgttct tggtagttaa gttgatccat cttggcttat gttgtgtgta 3480
tgttgtagtt attcttagta tattcctgtc ctgagtttag tgaaacataa tatcgccttg 3540
aaatgaaaat gctgaaattc gtcgacatac aatttttcaa actttttttt tttcttggtg 3600
cacggacatg tttttaaagg aagtactcta taccagttat tcttcacaaa tttaattgct 3660
ggagaataga tcttcaacgc tttaataaag tagtttgttt gtcaaggatg gcgtcataca 3720
aagaaagatc agaatcacac acttcccctg ttgctaggag acttttctcc atcatggagg 3780
aaaagaagtc taacctttgt gcatcattgg atattactga aactgaaaag cttctctcta 3840
ttttggacac tattggtcct tacatctgtc tagttaaaac acacatcgat attgtttctg 3900
attttacgta tgaaggaact gtgttgcctt tgaaggagct tgccaagaaa cataatttta 3960
tgatttttga agatagaaaa tttgctgata ttggtaacac tgttaaaaat caatataaat 4020
ctggtgtctt ccgtattgcc gaatgggctg acatcactaa tgcacatggt gtaacgggtg 4080
caggtattgt ttctggcttg aaggaggcag cccaagaaac aaccagtgaa cctagaggtt 4140
tgctaatgct tgctgagtta tcatcaaagg gttctttagc atatggtgaa tatacagaaa 4200
aaacagtaga aattgctaaa tctgataaag agtttgttga g                     4241

<210> 19
<211> 2466
<212> DNA
<213> Artificial Sequence

<220> 
<223> c-term URA3 gene integration fragment

<400> 19
aattctttga aggagcttgc caagaaacat aattttatga tttttgaaga tagaaaattt 60
gctgatattg gtaacactgt taaaaatcaa tataaatctg gtgtcttccg tattgccgaa 120
tgggctgaca tcactaatgc acatggtgta acgggtgcag gtattgtttc tggcttgaag 180
gaggcagccc aagaaacaac cagtgaacct agaggtttgc taatgcttgc tgagttatca 240
tcaaagggtt ctttagcata tggtgaatat acagaaaaaa cagtagaaat tgctaaatct 300
gataaagagt ttgtcattgg ttttattgcg caacacgata tgggcggtag agaagaaggt 360
tttgactgga tcattatgac tccaggggtt ggtttagatg acaaaggtga tgcacttggt 420
caacaatata gaactgttga tgaagttgta aagactggaa cggatatcat aattgttggt 480
agaggtttgt acggtcaagg aagagatcct atagagcaag ctaaaagata ccaacaagct 540
ggttggaatg cttatttaaa cagatttaaa tgattcttac acaaagattt gatacatgta 600
cactagttta aataagcatg aaaagaatta cacaagcaaa aaaaaaaaaa taaatgaggt 660
actttacgtt cacctacaac caaaaaaact agatagagta aaatcttaag atttagaaaa 720
agttgtttaa caaaggcttt agtatgtgaa tttttaatgt agcaaagcga taactaataa 780
acataaacaa aagtatggtt ttctttatca gtcaaatcat tatcgattga ttgttccgcg 840
tatctgcaga tagcctcatg aaatcagcca tttgcttttg ttcaacgatc ttttgaaatt 900
gttgttgttc ttggtagtta agttgatcca tcttggctta tgttgtgtgt atgttgtagt 960
tattcttagt atattcctgt cctgagttta gtgaaacata atatcgcctt gaaatgaaaa 1020
tgctgaaatt cgtcgacata caatttttca aacttttttt ttttcttggt gcacggacat 1080
gtttttaaag gaagtactct ataccagtta ttcttcacaa atttaattgc tggagaatag 1140
atcttcaacg cgtttggcgc gccatctaat agtttaatca cagcttatag tctactatag 1200
ttttcttttt taaacattgt tgtattttgt cccccccctc taattgatga tgattatcct 1260
ataagaatcc aataaaacga tggaaactaa taccctctcc tttgtcatgt ggtctttagt 1320
atttcttgaa cattggctct gatttctcga ctttatagtc ctattaaaat cgctgttagt 1380
tctcgatcgt tgtatctcgt ttcttgtctc tttggtggat gattttgcgt gcgaacatgt 1440
ttttttccct ttctctcacc atcatcgtgt agttcttgtc accatccccc ccaccccttc 1500
cttctctcat tgattctata agagcttatc cacagaggtg cagtaacgag gtagtttaac 1560
cttcgagtgg atcaaaatgt cacacaggcc tgcggccgca tttggcaagg cgtatctata 1620
taggaggatc acaagaaaag agagttcaac ttagggaacc aggcttgaca aaagataata 1680
ttaaaaaagg aagaaaaaga gagaaagaaa gtaaagacaa gaagaatcaa ctccaatttg 1740
aataaatggg cctgtaagaa tcttcaatct cgatgacggg gaaaacgtcc tcttttatag 1800
accaacccct tgtggggctg tcgggaaaag cgggtttccc gggaaactca tatctgattg 1860
gggggttgaa gctattccgc gttttggggg gtgcggttta taagaagaat gagtaatgca 1920
aacggcgttt ataaagagaa agggtgtggc ggtgttaagc ggttgaggtt ttaaggtgtg 1980
ggggacgggg tgtgattttt tcggcgttac gggcacgggg tatgattctc ttaatgtcat 2040
ttccctttct ttctacttct agggcgcgca gcgtgcgaca atggtgtttg ttgtgcaaat 2100
aggtggttgt gttgtcgttg gagttctcat gaacatatca ctctattagc aaatttaccc 2160
taagtatgta aggtttagtg tgttgaggac taatacagac aatctttgtc ttatatataa 2220
atggttctac tcaattttat aagttttttt tttttttttt taatctcttt atgaaaatca 2280
agaatgacat taaaacaatc acaatattgt atgcaagaac tttgcctcta aacctctttc 2340
aaagagatgc ataattataa aacaaatcta tttgcatatt cgctttacac aactattcaa 2400
gaatataatt acctaaagag tccatcttga gttcaccaac accactactt agagctcggt 2460
acccgc                                                            2466

<210> 20
<211> 3889
<212> DNA
<213> Artificial Sequence

<220> 
<223> GPD gene deletion fragment

<400> 20
ccttcattta cgaaataaag tgccgcggtt acgcagcaca caccagcaat cacgtgcagt 60
gtctttttct tttttttttc ttttttttcc tctttttctt ttgttttgtt tcgtttcttt 120
tccgccagtt cccgttttcc atttccggaa caacaatggg actccactgt tttctttccc 180
cccttccgtt ttcggctcgc agtctgtaca tgcacgttta tccgacacct gtcttgtttg 240
gcgcgtaatt aatacagttt ctccggagtc caggtctcgg acgggtaatt tacacgtcat 300
cattcatttc tgtgtcaaga gaggtagcgc aaaaagtaga aatggtgaac cacgggaatg 360
acttgctgga aatcgacgcc agagtccatt tgaaaaccta cctctacaag agaggaaaca 420
cactacaggg tgtccctggt ccgtaaaatg gcgtaatatg atgacttccc tctatagacg 480
ttgtatttcc agctccaaca tggttaaact attgctatgg tgatggtatt acagatagta 540
aaagaaggaa gggggggtgg caatctcacc ctaacagtta ctaagaacgt ctacttcatc 600
tactgtcaat atacattggc cacatgccga gaaattacgt cgacgccaaa gaagggctca 660
gccgaaaaaa gaaatggaaa acttggccga aaagggaaac aaacaaaaag gtgatgtaaa 720
attagcggaa aggggaattg gcaaattgag ggagaaaaaa aaaaggcaga aaaggaggcg 780
gaaagtcagt acgttttgaa ggcgtcattg gttttccctt ttgcagagtg tttcatttct 840
tttgtttcat gacgtagtgg cgtttctttt cctgcacttt agaaatctat cttttcctta 900
tcaagtaaca agcggttggc aaaggtgtat ataaatcaag gaattcccac tttgaaccct 960
ttgaattttg atatcgttta ttttaaattt atttgcggcc gcggatccct cgaggcctta 1020
attaacatct gaatgtaaaa tgaacattaa aatgaattac taaactttac gtctacttta 1080
caatctataa actttgttta atcatataac gaaatacact aatacacaat cctgtacgta 1140
tgtaatactt ttatccatca aggattgaga aaaaaaagta atgattccct gggccattaa 1200
aacttagacc cccaagcttg gataggtcac tctctatttt cgtttctccc ttccctgata 1260
gaagggtgat atgtaattaa gaataatata taattttata ataaaagaat tcatagcctc 1320
atgaaatcag ccatttgctt ttgttcaacg atcttttgaa attgttgttg ttcttggtag 1380
ttaagttgat ccatcttggc ttatgttgtg tgtatgttgt agttattctt agtatattcc 1440
tgtcctgagt ttagtgaaac ataatatcgc cttgaaatga aaatgctgaa attcgtcgac 1500
atacaatttt tcaaactttt tttttttctt ggtgcacgga catgttttta aaggaagtac 1560
tctataccag ttattcttca caaatttaat tgctggagaa tagatcttca acgctttaat 1620
aaagtagttt gtttgtcaag gatggcgtca tacaaagaaa gatcagaatc acacacttcc 1680
cctgttgcta ggagactttt ctccatcatg gaggaaaaga agtctaacct ttgtgcatca 1740
ttggatatta ctgaaactga aaagcttctc tctattttgg acactattgg tccttacatc 1800
tgtctagtta aaacacacat cgatattgtt tctgatttta cgtatgaagg aactgtgttg 1860
cctttgaagg agcttgccaa gaaacataat tttatgattt ttgaagatag aaaatttgct 1920
gatattggta acactgttaa aaatcaatat aaatctggtg tcttccgtat tgccgaatgg 1980
gctgacatca ctaatgcaca tggtgtaacg ggtgcaggta ttgtttctgg cttgaaggag 2040
gcagcccaag aaacaaccag tgaacctaga ggtttgctaa tgcttgctga gttatcatca 2100
aagggttctt tagcatatgg tgaatataca gaaaaaacag tagaaattgc taaatctgat 2160
aaagagtttg tcattggttt tattgcgcaa cacgatatgg gcggtagaga agaaggtttt 2220
gactggatca ttatgactcc aggggttggt ttagatgaca aaggtgatgc acttggtcaa 2280
caatatagaa ctgttgatga agttgtaaag actggaacgg atatcataat tgttggtaga 2340
ggtttgtacg gtcaaggaag agatcctata gagcaagcta aaagatacca acaagctggt 2400
tggaatgctt atttaaacag atttaaatga ttcttacaca aagatttgat acatgtacac 2460
tagtttaaat aagcatgaaa agaattacac aagcaaaaaa aaaaaaataa atgaggtact 2520
ttacgttcac ctacaaccaa aaaaactaga tagagtaaaa tcttaagatt tagaaaaagt 2580
tgtttaacaa aggctttagt atgtgaattt ttaatgtagc aaagcgataa ctaataaaca 2640
taaacaaaag tatggttttc tttatcagtc aaatcattat cgattgattg ttccgcgtat 2700
ctgcagatag cctcatgaaa tcagccattt gcttttgttc aacgatcttt tgaaattgtt 2760
gttgttcttg gtagttaagt tgatccatct tggcttatgt tgtgtgtatg ttgtagttat 2820
tcttagtata ttcctgtcct gagtttagtg aaacataata tcgccttgaa atgaaaatgc 2880
tgaaattcgt cgacatacaa tttttcaaac tttttttttt tcttggtgca cggacatgtt 2940
tttaaaggaa gtactctata ccagttattc ttcacaaatt taattgctgg agaatagatc 3000
ttcaacgccc cgggggatct ggatccgcgg ccgcaataac ctcagggaga actttggcat 3060
tgtactctcc attgacgagt ccgccaaccc attcttgtta aacctaacct tgcattatca 3120
cattcccttt gacccccttt agctgcattt ccacttgtct acattaagat tcattacaca 3180
ttctttttcg tatttctctt acctccctcc cccctccatg gatcttatat ataaatcttt 3240
tctataacaa taatatctac tagagttaaa caacaattcc acttggcatg gctgtctcag 3300
caaatctgct tctacctact gcacgggttt gcatgtcatt gtttctagca gggaatcgtc 3360
catgtacgtt gtcctccatg atggtcttcc cgctgccact ttctttagta tcttaaatag 3420
agcagatctt acgtccactg tgcatccgtg caccccgaaa atcgtatggt tttccttgcc 3480
acctctcaca attttgaata tgctcaacgc gaaagagagg ggaagaggaa tcgcattcgt 3540
agagtggcta cattcaaccc tgacaaagga actagcgttt gtgcaggaga gagtggtttg 3600
catagatttc ctttcctttg caagcatatt atatagagta gccaatacag taacagctac 3660
agcacaaaaa agagaacgag aacgagaacg agaacaagaa caagaactag cactactgtc 3720
actgccagca tcaacattac taccattatt ccaacatgtt tgcaactaga aatataacca 3780
ttggtgtcag aacactcaga ccaaccagtt tcttgaaaac aaggtctttt ctgcaacaga 3840
ggctacaatc aacgctaaag aagagctatg aaccaaccaa atccgagct             3889

<210> 21
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 21
tggcccaggg aatcattac                                              19

<210> 22
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 22
tcaccacctg tcagtgacga gccacttc                                    28

<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 23
ggacccaatg cctcccaatc                                             20

<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> primer

<400> 24
cgtttctccc ttccctgata g                                           21

<210> 25
<211> 3889
<212> DNA
<213> Artificial Sequence

<220> 
<223> GPD gene deletion fragment

<400> 25
ccttcattta cgaaataaag tgccgcggtt acgcagcaca caccagcaat cacgtgcagt 60
gtctttttct tttttttttc ttttttttcc tctttttctt ttgttttgtt tcgtttcttt 120
tccgccagtt cccgttttcc atttccggaa caacaatggg actccactgt tttctttccc 180
cccttccgtt ttcggctcgc agtctgtaca tgcacgttta tccgacacct gtcttgtttg 240
gcgcgtaatt aatacagttt ctccggagtc caggtctcgg acgggtaatt tacacgtcat 300
cattcatttc tgtgtcaaga gaggtagcgc aaaaagtaga aatggtgaac cacgggaatg 360
acttgctgga aatcgacgcc agagtccatt tgaaaaccta cctctacaag agaggaaaca 420
cactacaggg tgtccctggt ccgtaaaatg gcgtaatatg atgacttccc tctatagacg 480
ttgtatttcc agctccaaca tggttaaact attgctatgg tgatggtatt acagatagta 540
aaagaaggaa gggggggtgg caatctcacc ctaacagtta ctaagaacgt ctacttcatc 600
tactgtcaat atacattggc cacatgccga gaaattacgt cgacgccaaa gaagggctca 660
gccgaaaaaa gaaatggaaa acttggccga aaagggaaac aaacaaaaag gtgatgtaaa 720
attagcggaa aggggaattg gcaaattgag ggagaaaaaa aaaaggcaga aaaggaggcg 780
gaaagtcagt acgttttgaa ggcgtcattg gttttccctt ttgcagagtg tttcatttct 840
tttgtttcat gacgtagtgg cgtttctttt cctgcacttt agaaatctat cttttcctta 900
tcaagtaaca agcggttggc aaaggtgtat ataaatcaag gaattcccac tttgaaccct 960
ttgaattttg atatcgttta ttttaaattt atttgcggcc gcggatccag atcccccggg 1020
gcgttgaaga tctattctcc agcaattaaa tttgtgaaga ataactggta tagagtactt 1080
cctttaaaaa catgtccgtg caccaagaaa aaaaaaaagt ttgaaaaatt gtatgtcgac 1140
gaatttcagc attttcattt caaggcgata ttatgtttca ctaaactcag gacaggaata 1200
tactaagaat aactacaaca tacacacaac ataagccaag atggatcaac ttaactacca 1260
agaacaacaa caatttcaaa agatcgttga acaaaagcaa atggctgatt tcatgaggct 1320
atctgcagat acgcggaaca atcaatcgat aatgatttga ctgataaaga aaaccatact 1380
tttgtttatg tttattagtt atcgctttgc tacattaaaa attcacatac taaagccttt 1440
gttaaacaac tttttctaaa tcttaagatt ttactctatc tagttttttt ggttgtaggt 1500
gaacgtaaag tacctcattt attttttttt ttttgcttgt gtaattcttt tcatgcttat 1560
ttaaactagt gtacatgtat caaatctttg tgtaagaatc atttaaatct gtttaaataa 1620
gcattccaac cagcttgttg gtatctttta gcttgctcta taggatctct tccttgaccg 1680
tacaaacctc taccaacaat tatgatatcc gttccagtct ttacaacttc atcaacagtt 1740
ctatattgtt gaccaagtgc atcacctttg tcatctaaac caacccctgg agtcataatg 1800
atccagtcaa aaccttcttc tctaccgccc atatcgtgtt gcgcaataaa accaatgaca 1860
aactctttat cagatttagc aatttctact gttttttctg tatattcacc atatgctaaa 1920
gaaccctttg atgataactc agcaagcatt agcaaacctc taggttcact ggttgtttct 1980
tgggctgcct ccttcaagcc agaaacaata cctgcacccg ttacaccatg tgcattagtg 2040
atgtcagccc attcggcaat acggaagaca ccagatttat attgattttt aacagtgtta 2100
ccaatatcag caaattttct atcttcaaaa atcataaaat tatgtttctt ggcaagctcc 2160
ttcaaaggca acacagttcc ttcatacgta aaatcagaaa caatatcgat gtgtgtttta 2220
actagacaga tgtaaggacc aatagtgtcc aaaatagaga gaagcttttc agtttcagta 2280
atatccaatg atgcacaaag gttagacttc ttttcctcca tgatggagaa aagtctccta 2340
gcaacagggg aagtgtgtga ttctgatctt tctttgtatg acgccatcct tgacaaacaa 2400
actactttat taaagcgttg aagatctatt ctccagcaat taaatttgtg aagaataact 2460
ggtatagagt acttccttta aaaacatgtc cgtgcaccaa gaaaaaaaaa aagtttgaaa 2520
aattgtatgt cgacgaattt cagcattttc atttcaaggc gatattatgt ttcactaaac 2580
tcaggacagg aatatactaa gaataactac aacatacaca caacataagc caagatggat 2640
caacttaact accaagaaca acaacaattt caaaagatcg ttgaacaaaa gcaaatggct 2700
gatttcatga ggctatgaat tcttttatta taaaattata tattattctt aattacatat 2760
cacccttcta tcagggaagg gagaaacgaa aatagagagt gacctatcca agcttggggg 2820
tctaagtttt aatggcccag ggaatcatta cttttttttc tcaatccttg atggataaaa 2880
gtattacata cgtacaggat tgtgtattag tgtatttcgt tatatgatta aacaaagttt 2940
atagattgta aagtagacgt aaagtttagt aattcatttt aatgttcatt ttacattcag 3000
atgttaatta aggcctcgag ggatccgcgg ccgcaataac ctcagggaga actttggcat 3060
tgtactctcc attgacgagt ccgccaaccc attcttgtta aacctaacct tgcattatca 3120
cattcccttt gacccccttt agctgcattt ccacttgtct acattaagat tcattacaca 3180
ttctttttcg tatttctctt acctccctcc cccctccatg gatcttatat ataaatcttt 3240
tctataacaa taatatctac tagagttaaa caacaattcc acttggcatg gctgtctcag 3300
caaatctgct tctacctact gcacgggttt gcatgtcatt gtttctagca gggaatcgtc 3360
catgtacgtt gtcctccatg atggtcttcc cgctgccact ttctttagta tcttaaatag 3420
agcagatctt acgtccactg tgcatccgtg caccccgaaa atcgtatggt tttccttgcc 3480
acctctcaca attttgaata tgctcaacgc gaaagagagg ggaagaggaa tcgcattcgt 3540
agagtggcta cattcaaccc tgacaaagga actagcgttt gtgcaggaga gagtggtttg 3600
catagatttc ctttcctttg caagcatatt atatagagta gccaatacag taacagctac 3660
agcacaaaaa agagaacgag aacgagaacg agaacaagaa caagaactag cactactgtc 3720
actgccagca tcaacattac taccattatt ccaacatgtt tgcaactaga aatataacca 3780
ttggtgtcag aacactcaga ccaaccagtt tcttgaaaac aaggtctttt ctgcaacaga 3840
ggctacaatc aacgctaaag aagagctatg aaccaaccaa atccgagct             3889
