                         SEQUENCE LISTING

<110>  CHILDREN'S NATIONAL MEDICAL CENTER
       BOLLARD, Catherine
       LAM, Sharon
       CRUZ, Conrad Russell
 
<120>  GENERATION OF BROADLY-SPECIFIC, VIRUS-IMMUNE CELLS TARGETING 
       MULTIPLE HIV ANTIGENS FOR PREVENTIVE AND THERAPEUTIC USE

<130>  436931WO

<150>  US 62/011,393
<151>  2014-06-12

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2910
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (164)..(2677)

<400>  1
acttccggcg ggaggcggag gcggaggcgc aggcgctggc tggcaggtgt cgctaaccgg       60

acggtggtcg ccagggcgag aggcgggagc cggagaggtg aggcaggacc cgggctccac      120

tgccgcctct ccgagctctt gtgacgcgga cctcagtgcc agg atg gct cgg ggc        175
                                                Met Ala Arg Gly           
                                                1                         

gag cgg cgg cgc cgc gca gtg ccg gca gag gga gtg cgg aca gcc gag        223
Glu Arg Arg Arg Arg Ala Val Pro Ala Glu Gly Val Arg Thr Ala Glu           
5                   10                  15                  20            

agg gcg gct cgg gga ggc ccc ggg cga cgg gac ggc cgg ggc ggc ggg        271
Arg Ala Ala Arg Gly Gly Pro Gly Arg Arg Asp Gly Arg Gly Gly Gly           
                25                  30                  35                

ccg cgt agc acg gct gga gga gtg gct ctg gcc gtc gtg gtc ctg tct        319
Pro Arg Ser Thr Ala Gly Gly Val Ala Leu Ala Val Val Val Leu Ser           
            40                  45                  50                    

ttg gcc ctg ggt atg tcg ggg cgc tgg gtg ctg gcg tgg tac cgt gcg        367
Leu Ala Leu Gly Met Ser Gly Arg Trp Val Leu Ala Trp Tyr Arg Ala           
        55                  60                  65                        

cgg cgg gcg gtc acg ctg cac tcc gcg cct cct gtg ttg cct gcc gac        415
Arg Arg Ala Val Thr Leu His Ser Ala Pro Pro Val Leu Pro Ala Asp           
    70                  75                  80                            

tcc tcc agc ccc gcc gtg gcc ccg gac ctc ttc tgg gga acc tac cgc        463
Ser Ser Ser Pro Ala Val Ala Pro Asp Leu Phe Trp Gly Thr Tyr Arg           
85                  90                  95                  100           

cct cac gtc tac ttc ggc atg aag acc cgc agc ccg aag ccc ctc ctc        511
Pro His Val Tyr Phe Gly Met Lys Thr Arg Ser Pro Lys Pro Leu Leu           
                105                 110                 115               

acc gga ctg atg tgg gcg cag cag ggc acc acc ccg ggg act cct aag        559
Thr Gly Leu Met Trp Ala Gln Gln Gly Thr Thr Pro Gly Thr Pro Lys           
            120                 125                 130                   

ctc agg cac acg tgt gag cag ggg gac ggt gtg ggt ccc tat ggc tgg        607
Leu Arg His Thr Cys Glu Gln Gly Asp Gly Val Gly Pro Tyr Gly Trp           
        135                 140                 145                       

gag ttc cac gac ggc ctc tcc ttc ggg cgc caa cac atc cag gat ggg        655
Glu Phe His Asp Gly Leu Ser Phe Gly Arg Gln His Ile Gln Asp Gly           
    150                 155                 160                           

gcc tta agg ctc acc act gag ttc gtc aag agg cct ggg ggt cag cac        703
Ala Leu Arg Leu Thr Thr Glu Phe Val Lys Arg Pro Gly Gly Gln His           
165                 170                 175                 180           

gga ggg gac tgg agc tgg aga gtg act gta gag cct cag gac tca ggt        751
Gly Gly Asp Trp Ser Trp Arg Val Thr Val Glu Pro Gln Asp Ser Gly           
                185                 190                 195               

act tct gcc ctc cct ttg gtc tcc ctg ttc ttc tat gtg gtg aca gat        799
Thr Ser Ala Leu Pro Leu Val Ser Leu Phe Phe Tyr Val Val Thr Asp           
            200                 205                 210                   

ggc aag gaa gtc cta cta cca gag gtt ggg gcc aag ggg cag ttg aag        847
Gly Lys Glu Val Leu Leu Pro Glu Val Gly Ala Lys Gly Gln Leu Lys           
        215                 220                 225                       

ttt atc agt ggg cac acc agt gaa ctt ggt gac ttc cgc ttt aca ctt        895
Phe Ile Ser Gly His Thr Ser Glu Leu Gly Asp Phe Arg Phe Thr Leu           
    230                 235                 240                           

ttg cca cca acc agt cca ggg gat aca gcc ccc aag tat ggc agc tac        943
Leu Pro Pro Thr Ser Pro Gly Asp Thr Ala Pro Lys Tyr Gly Ser Tyr           
245                 250                 255                 260           

aat gtc ttc tgg acc tcc aac cca gga ctg ccc ctg ctg aca gag atg        991
Asn Val Phe Trp Thr Ser Asn Pro Gly Leu Pro Leu Leu Thr Glu Met           
                265                 270                 275               

gta aag agt cgc cta aat agc tgg ttt cag cat cgg ccc cca ggg gcc       1039
Val Lys Ser Arg Leu Asn Ser Trp Phe Gln His Arg Pro Pro Gly Ala           
            280                 285                 290                   

ccc cct gaa cgc tac ctc ggc ttg cca gga tcc ctg aag tgg gag gac       1087
Pro Pro Glu Arg Tyr Leu Gly Leu Pro Gly Ser Leu Lys Trp Glu Asp           
        295                 300                 305                       

aga ggt cca agt ggg caa ggg cag ggg cag ttc ttg ata cag cag gtg       1135
Arg Gly Pro Ser Gly Gln Gly Gln Gly Gln Phe Leu Ile Gln Gln Val           
    310                 315                 320                           

acc ctg aaa att ccc att tcc ata gag ttt gtg ttt gaa tca ggc agt       1183
Thr Leu Lys Ile Pro Ile Ser Ile Glu Phe Val Phe Glu Ser Gly Ser           
325                 330                 335                 340           

gcc cag gca gga gga aat caa gcc ctg cca aga ctg gca ggc agt cta       1231
Ala Gln Ala Gly Gly Asn Gln Ala Leu Pro Arg Leu Ala Gly Ser Leu           
                345                 350                 355               

ctg acc cag gcc ctg gag agc cat gct gaa ggc ttt aga gag cgc ttt       1279
Leu Thr Gln Ala Leu Glu Ser His Ala Glu Gly Phe Arg Glu Arg Phe           
            360                 365                 370                   

gag aag acc ttc cag ctg aag gag aag ggc ctg agc tct ggc gag cag       1327
Glu Lys Thr Phe Gln Leu Lys Glu Lys Gly Leu Ser Ser Gly Glu Gln           
        375                 380                 385                       

gtt ttg ggt cag gct gcc ctc agc ggc ctc ctt ggt gga att ggc tac       1375
Val Leu Gly Gln Ala Ala Leu Ser Gly Leu Leu Gly Gly Ile Gly Tyr           
    390                 395                 400                           

ttc tac gga caa ggg ctg gta ttg cca gac atc ggg gtg gaa ggg tct       1423
Phe Tyr Gly Gln Gly Leu Val Leu Pro Asp Ile Gly Val Glu Gly Ser           
405                 410                 415                 420           

gag cag aag gtg gac cca gcc ctc ttt cca ccc gta cct ctt ttt aca       1471
Glu Gln Lys Val Asp Pro Ala Leu Phe Pro Pro Val Pro Leu Phe Thr           
                425                 430                 435               

gca gtg ccc tcc cgg tca ttc ttc cca cga ggc ttc ctt tgg gat gaa       1519
Ala Val Pro Ser Arg Ser Phe Phe Pro Arg Gly Phe Leu Trp Asp Glu           
            440                 445                 450                   

ggc ttt cac cag ctg gtg gtt cag cgg tgg gat ccc tcc ctc acc cgg       1567
Gly Phe His Gln Leu Val Val Gln Arg Trp Asp Pro Ser Leu Thr Arg           
        455                 460                 465                       

gaa gcc ctt ggc cac tgg ctg ggg ctg cta aat gct gat ggc tgg att       1615
Glu Ala Leu Gly His Trp Leu Gly Leu Leu Asn Ala Asp Gly Trp Ile           
    470                 475                 480                           

ggg agg gag cag ata ctg ggg gat gag gcc cga gcc cgg gtg cct cca       1663
Gly Arg Glu Gln Ile Leu Gly Asp Glu Ala Arg Ala Arg Val Pro Pro           
485                 490                 495                 500           

gaa ttc cta gta caa cga gca gtc cac gcc aac ccc cca acc cta ctt       1711
Glu Phe Leu Val Gln Arg Ala Val His Ala Asn Pro Pro Thr Leu Leu           
                505                 510                 515               

ttg cct gta gcc cat atg cta gag gtt ggt gac cct gac gac ttg gct       1759
Leu Pro Val Ala His Met Leu Glu Val Gly Asp Pro Asp Asp Leu Ala           
            520                 525                 530                   

ttc ctc cga aag gcc ttg ccc cgc ctg cat gcc tgg ttt tcc tgg ctc       1807
Phe Leu Arg Lys Ala Leu Pro Arg Leu His Ala Trp Phe Ser Trp Leu           
        535                 540                 545                       

cat cag agc cag gca ggc cca ctg cca cta tct tac cgc tgg cgg gga       1855
His Gln Ser Gln Ala Gly Pro Leu Pro Leu Ser Tyr Arg Trp Arg Gly           
    550                 555                 560                           

cgg gac cct gcc tta cca acc tta ctg aac ccc aag acc cta ccc tct       1903
Arg Asp Pro Ala Leu Pro Thr Leu Leu Asn Pro Lys Thr Leu Pro Ser           
565                 570                 575                 580           

ggg ctg gat gac tac ccc cgg gct tca cac cct tca gta acc gag cgg       1951
Gly Leu Asp Asp Tyr Pro Arg Ala Ser His Pro Ser Val Thr Glu Arg           
                585                 590                 595               

cac ctg gac ctg cga tgt tgg gtg gca ctg ggt gcc cgt gtg ctg acg       1999
His Leu Asp Leu Arg Cys Trp Val Ala Leu Gly Ala Arg Val Leu Thr           
            600                 605                 610                   

cgg ctg gca gag cat ctg ggt gag gct gag gta gct gct gag ctg ggc       2047
Arg Leu Ala Glu His Leu Gly Glu Ala Glu Val Ala Ala Glu Leu Gly           
        615                 620                 625                       

cca ctg gct gcc tca ctg gag gca gca gag agc ctg gat gag ctg cac       2095
Pro Leu Ala Ala Ser Leu Glu Ala Ala Glu Ser Leu Asp Glu Leu His           
    630                 635                 640                           

tgg gcc cca gag cta gga gtc ttt gca gac ttt ggg aac cac aca aaa       2143
Trp Ala Pro Glu Leu Gly Val Phe Ala Asp Phe Gly Asn His Thr Lys           
645                 650                 655                 660           

gca gta cag ctg aag ccc agg ccc cct cag ggg ctc gtt cgg gtg gtg       2191
Ala Val Gln Leu Lys Pro Arg Pro Pro Gln Gly Leu Val Arg Val Val           
                665                 670                 675               

ggt cgg ccc caa cct caa ctg cag tat gta gat gct ctt ggc tat gtc       2239
Gly Arg Pro Gln Pro Gln Leu Gln Tyr Val Asp Ala Leu Gly Tyr Val           
            680                 685                 690                   

agt ctt ttt ccc ttg ctg ctg cga ctg ctg gac ccc acc tca tcc cgc       2287
Ser Leu Phe Pro Leu Leu Leu Arg Leu Leu Asp Pro Thr Ser Ser Arg           
        695                 700                 705                       

ctt ggg ccc ctg ctg gac att cta gcc gac agc cgc cat ctc tgg agc       2335
Leu Gly Pro Leu Leu Asp Ile Leu Ala Asp Ser Arg His Leu Trp Ser           
    710                 715                 720                           

ccc ttt ggt tta cgc tcc ctt gca gcc tcc agc tcc ttt tat ggc cag       2383
Pro Phe Gly Leu Arg Ser Leu Ala Ala Ser Ser Ser Phe Tyr Gly Gln           
725                 730                 735                 740           

cgc aat tca gag cat gat ccc ccc tac tgg cgg ggt gct gtg tgg ctc       2431
Arg Asn Ser Glu His Asp Pro Pro Tyr Trp Arg Gly Ala Val Trp Leu           
                745                 750                 755               

aat gtc aac tac ctg gct ttg gga gca ctc cac cac tat ggg cat ctg       2479
Asn Val Asn Tyr Leu Ala Leu Gly Ala Leu His His Tyr Gly His Leu           
            760                 765                 770                   

gag ggt cct cac cag gct cgg gct gcc aaa ctc cac ggt gag ctc cgt       2527
Glu Gly Pro His Gln Ala Arg Ala Ala Lys Leu His Gly Glu Leu Arg           
        775                 780                 785                       

gcc aac gtg gta ggc aat gta tgg cgc cag tac cag gct aca ggc ttt       2575
Ala Asn Val Val Gly Asn Val Trp Arg Gln Tyr Gln Ala Thr Gly Phe           
    790                 795                 800                           

ctt tgg gag cag tac agt gac cgc gat ggg cga ggc atg ggc tgc cgc       2623
Leu Trp Glu Gln Tyr Ser Asp Arg Asp Gly Arg Gly Met Gly Cys Arg           
805                 810                 815                 820           

cct ttc cac ggc tgg acc agc ctt gtc tta ctg gcc atg gct gaa gac       2671
Pro Phe His Gly Trp Thr Ser Leu Val Leu Leu Ala Met Ala Glu Asp           
                825                 830                 835               

tac tga agggagggag aggaggggag ccaagacact catgccactc tggctctgaa        2727
Tyr                                                                       
                                                                          

gggacaaagg cttctggctt ttgcccccag ccccttggat accagtaatt caaaccttcc     2787

tcatttcatc tcaggtgtct ccttgctgtc atcccacata gccctggggt gaatgtgaat     2847

ccagagtcta tttttctaaa taaattggaa aaaacatttt gaactctaaa aaaaaaaaaa     2907

aaa                                                                   2910


<210>  2
<211>  837
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Ala Arg Gly Glu Arg Arg Arg Arg Ala Val Pro Ala Glu Gly Val 
1               5                   10                  15      


Arg Thr Ala Glu Arg Ala Ala Arg Gly Gly Pro Gly Arg Arg Asp Gly 
            20                  25                  30          


Arg Gly Gly Gly Pro Arg Ser Thr Ala Gly Gly Val Ala Leu Ala Val 
        35                  40                  45              


Val Val Leu Ser Leu Ala Leu Gly Met Ser Gly Arg Trp Val Leu Ala 
    50                  55                  60                  


Trp Tyr Arg Ala Arg Arg Ala Val Thr Leu His Ser Ala Pro Pro Val 
65                  70                  75                  80  


Leu Pro Ala Asp Ser Ser Ser Pro Ala Val Ala Pro Asp Leu Phe Trp 
                85                  90                  95      


Gly Thr Tyr Arg Pro His Val Tyr Phe Gly Met Lys Thr Arg Ser Pro 
            100                 105                 110         


Lys Pro Leu Leu Thr Gly Leu Met Trp Ala Gln Gln Gly Thr Thr Pro 
        115                 120                 125             


Gly Thr Pro Lys Leu Arg His Thr Cys Glu Gln Gly Asp Gly Val Gly 
    130                 135                 140                 


Pro Tyr Gly Trp Glu Phe His Asp Gly Leu Ser Phe Gly Arg Gln His 
145                 150                 155                 160 


Ile Gln Asp Gly Ala Leu Arg Leu Thr Thr Glu Phe Val Lys Arg Pro 
                165                 170                 175     


Gly Gly Gln His Gly Gly Asp Trp Ser Trp Arg Val Thr Val Glu Pro 
            180                 185                 190         


Gln Asp Ser Gly Thr Ser Ala Leu Pro Leu Val Ser Leu Phe Phe Tyr 
        195                 200                 205             


Val Val Thr Asp Gly Lys Glu Val Leu Leu Pro Glu Val Gly Ala Lys 
    210                 215                 220                 


Gly Gln Leu Lys Phe Ile Ser Gly His Thr Ser Glu Leu Gly Asp Phe 
225                 230                 235                 240 


Arg Phe Thr Leu Leu Pro Pro Thr Ser Pro Gly Asp Thr Ala Pro Lys 
                245                 250                 255     


Tyr Gly Ser Tyr Asn Val Phe Trp Thr Ser Asn Pro Gly Leu Pro Leu 
            260                 265                 270         


Leu Thr Glu Met Val Lys Ser Arg Leu Asn Ser Trp Phe Gln His Arg 
        275                 280                 285             


Pro Pro Gly Ala Pro Pro Glu Arg Tyr Leu Gly Leu Pro Gly Ser Leu 
    290                 295                 300                 


Lys Trp Glu Asp Arg Gly Pro Ser Gly Gln Gly Gln Gly Gln Phe Leu 
305                 310                 315                 320 


Ile Gln Gln Val Thr Leu Lys Ile Pro Ile Ser Ile Glu Phe Val Phe 
                325                 330                 335     


Glu Ser Gly Ser Ala Gln Ala Gly Gly Asn Gln Ala Leu Pro Arg Leu 
            340                 345                 350         


Ala Gly Ser Leu Leu Thr Gln Ala Leu Glu Ser His Ala Glu Gly Phe 
        355                 360                 365             


Arg Glu Arg Phe Glu Lys Thr Phe Gln Leu Lys Glu Lys Gly Leu Ser 
    370                 375                 380                 


Ser Gly Glu Gln Val Leu Gly Gln Ala Ala Leu Ser Gly Leu Leu Gly 
385                 390                 395                 400 


Gly Ile Gly Tyr Phe Tyr Gly Gln Gly Leu Val Leu Pro Asp Ile Gly 
                405                 410                 415     


Val Glu Gly Ser Glu Gln Lys Val Asp Pro Ala Leu Phe Pro Pro Val 
            420                 425                 430         


Pro Leu Phe Thr Ala Val Pro Ser Arg Ser Phe Phe Pro Arg Gly Phe 
        435                 440                 445             


Leu Trp Asp Glu Gly Phe His Gln Leu Val Val Gln Arg Trp Asp Pro 
    450                 455                 460                 


Ser Leu Thr Arg Glu Ala Leu Gly His Trp Leu Gly Leu Leu Asn Ala 
465                 470                 475                 480 


Asp Gly Trp Ile Gly Arg Glu Gln Ile Leu Gly Asp Glu Ala Arg Ala 
                485                 490                 495     


Arg Val Pro Pro Glu Phe Leu Val Gln Arg Ala Val His Ala Asn Pro 
            500                 505                 510         


Pro Thr Leu Leu Leu Pro Val Ala His Met Leu Glu Val Gly Asp Pro 
        515                 520                 525             


Asp Asp Leu Ala Phe Leu Arg Lys Ala Leu Pro Arg Leu His Ala Trp 
    530                 535                 540                 


Phe Ser Trp Leu His Gln Ser Gln Ala Gly Pro Leu Pro Leu Ser Tyr 
545                 550                 555                 560 


Arg Trp Arg Gly Arg Asp Pro Ala Leu Pro Thr Leu Leu Asn Pro Lys 
                565                 570                 575     


Thr Leu Pro Ser Gly Leu Asp Asp Tyr Pro Arg Ala Ser His Pro Ser 
            580                 585                 590         


Val Thr Glu Arg His Leu Asp Leu Arg Cys Trp Val Ala Leu Gly Ala 
        595                 600                 605             


Arg Val Leu Thr Arg Leu Ala Glu His Leu Gly Glu Ala Glu Val Ala 
    610                 615                 620                 


Ala Glu Leu Gly Pro Leu Ala Ala Ser Leu Glu Ala Ala Glu Ser Leu 
625                 630                 635                 640 


Asp Glu Leu His Trp Ala Pro Glu Leu Gly Val Phe Ala Asp Phe Gly 
                645                 650                 655     


Asn His Thr Lys Ala Val Gln Leu Lys Pro Arg Pro Pro Gln Gly Leu 
            660                 665                 670         


Val Arg Val Val Gly Arg Pro Gln Pro Gln Leu Gln Tyr Val Asp Ala 
        675                 680                 685             


Leu Gly Tyr Val Ser Leu Phe Pro Leu Leu Leu Arg Leu Leu Asp Pro 
    690                 695                 700                 


Thr Ser Ser Arg Leu Gly Pro Leu Leu Asp Ile Leu Ala Asp Ser Arg 
705                 710                 715                 720 


His Leu Trp Ser Pro Phe Gly Leu Arg Ser Leu Ala Ala Ser Ser Ser 
                725                 730                 735     


Phe Tyr Gly Gln Arg Asn Ser Glu His Asp Pro Pro Tyr Trp Arg Gly 
            740                 745                 750         


Ala Val Trp Leu Asn Val Asn Tyr Leu Ala Leu Gly Ala Leu His His 
        755                 760                 765             


Tyr Gly His Leu Glu Gly Pro His Gln Ala Arg Ala Ala Lys Leu His 
    770                 775                 780                 


Gly Glu Leu Arg Ala Asn Val Val Gly Asn Val Trp Arg Gln Tyr Gln 
785                 790                 795                 800 


Ala Thr Gly Phe Leu Trp Glu Gln Tyr Ser Asp Arg Asp Gly Arg Gly 
                805                 810                 815     


Met Gly Cys Arg Pro Phe His Gly Trp Thr Ser Leu Val Leu Leu Ala 
            820                 825                 830         


Met Ala Glu Asp Tyr 
        835         


