                         SEQUENCE LISTING

<110>  ROQUETTE FRERES
 
<120>  PRODUCTION OF XYLITOL FROM GLUCOSE BY A RECOMBINANT STRAIN

<130>  B1828EP

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  1385
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Sequence encoding NAD-specific D-arabitol 4-oxidoreductase 
       flanked by restriction site


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  AscI recognition site

<220>
<221>  CDS
<222>  (12)..(1379)

<220>
<221>  misc_feature
<222>  (1380)..(1385)
<223>  SphI recognition site

<400>  1
ggcgcgccaa a atg aac gag cag ttc acc tgg ttg cac atc ggt ttg ggt        50
             Met Asn Glu Gln Phe Thr Trp Leu His Ile Gly Leu Gly          
             1               5                   10                       

tct ttc cac aga gct cac cag gct tgg tac ttg cac aga ttg cag gtt         98
Ser Phe His Arg Ala His Gln Ala Trp Tyr Leu His Arg Leu Gln Val           
    15                  20                  25                            

atg ggt gac aag aga tgg tct atc gct gct ggt aac atc aga aac gac        146
Met Gly Asp Lys Arg Trp Ser Ile Ala Ala Gly Asn Ile Arg Asn Asp           
30                  35                  40                  45            

gct gag cac gtt gtt cag gct ttg tct gct cag aag ggt aga tac gtt        194
Ala Glu His Val Val Gln Ala Leu Ser Ala Gln Lys Gly Arg Tyr Val           
                50                  55                  60                

ttg gag acc gtt tct cca gag ggt gtt tct gag tac gag gag atc acc        242
Leu Glu Thr Val Ser Pro Glu Gly Val Ser Glu Tyr Glu Glu Ile Thr           
            65                  70                  75                    

tct atc cag aag ttg atc cca tgg cag gct gac ttg cag cca ttg atc        290
Ser Ile Gln Lys Leu Ile Pro Trp Gln Ala Asp Leu Gln Pro Leu Ile           
        80                  85                  90                        

gct gag ggt gct gac cca aag acc aag gtt atc gct ttc acc gtt acc        338
Ala Glu Gly Ala Asp Pro Lys Thr Lys Val Ile Ala Phe Thr Val Thr           
    95                  100                 105                           

gag ggt ggt tac tac ttg aac acc tct cac aag ttg gag gtt aac aac        386
Glu Gly Gly Tyr Tyr Leu Asn Thr Ser His Lys Leu Glu Val Asn Asn           
110                 115                 120                 125           

cca gac ttg gct gct gac ttg aag ggt ggt tgt aag acc atc tac ggt        434
Pro Asp Leu Ala Ala Asp Leu Lys Gly Gly Cys Lys Thr Ile Tyr Gly           
                130                 135                 140               

gtt atc acc aga atc ttg gag gct aga atg gct aac aac gct ggt cca        482
Val Ile Thr Arg Ile Leu Glu Ala Arg Met Ala Asn Asn Ala Gly Pro           
            145                 150                 155                   

ttg acc ttg ttg aac tgt gac aac gtt aga cac aac ggt gag aga ttc        530
Leu Thr Leu Leu Asn Cys Asp Asn Val Arg His Asn Gly Glu Arg Phe           
        160                 165                 170                       

cac gac ggt ttg gtt gag ttc ttg cag ttg acc ggt aag cag gac gtt        578
His Asp Gly Leu Val Glu Phe Leu Gln Leu Thr Gly Lys Gln Asp Val           
    175                 180                 185                           

atc gac tgg ttg tct acc aac acc acc tgt cca aac acc atg gtt gac        626
Ile Asp Trp Leu Ser Thr Asn Thr Thr Cys Pro Asn Thr Met Val Asp           
190                 195                 200                 205           

aga atc acc cca aga cca gct gct gag ttg cca gct aga atc aag gct        674
Arg Ile Thr Pro Arg Pro Ala Ala Glu Leu Pro Ala Arg Ile Lys Ala           
                210                 215                 220               

cag acc ggt atc gct gac aag gct cca gtt atg ggt gag acc ttc atc        722
Gln Thr Gly Ile Ala Asp Lys Ala Pro Val Met Gly Glu Thr Phe Ile           
            225                 230                 235                   

cag tgg gtt gtt gag gac aac ttc aga gac gtt aga cca gct ttg gag        770
Gln Trp Val Val Glu Asp Asn Phe Arg Asp Val Arg Pro Ala Leu Glu           
        240                 245                 250                       

aag gtt ggt gtt gag ttg gtt gct tct gtt atc cca tac gag gag gct        818
Lys Val Gly Val Glu Leu Val Ala Ser Val Ile Pro Tyr Glu Glu Ala           
    255                 260                 265                           

aag atc aga atc ttg aac tct tct cac tct tgt atc gct tgg gct ggt        866
Lys Ile Arg Ile Leu Asn Ser Ser His Ser Cys Ile Ala Trp Ala Gly           
270                 275                 280                 285           

acc ttg atc ggt cag aag tac atc cac gag tct acc atg acc gac ttc        914
Thr Leu Ile Gly Gln Lys Tyr Ile His Glu Ser Thr Met Thr Asp Phe           
                290                 295                 300               

atc tac cag atc gct gac aga tac gtt acc gag gac gtt atc cca tgt        962
Ile Tyr Gln Ile Ala Asp Arg Tyr Val Thr Glu Asp Val Ile Pro Cys           
            305                 310                 315                   

ttg ggt gac aac ggt atc gac ttg cca acc tac aga gac gtt gtt ttg       1010
Leu Gly Asp Asn Gly Ile Asp Leu Pro Thr Tyr Arg Asp Val Val Leu           
        320                 325                 330                       

aag aga ttc acc aac cca cac atc cag gac acc aac cag aga gtt gct       1058
Lys Arg Phe Thr Asn Pro His Ile Gln Asp Thr Asn Gln Arg Val Ala           
    335                 340                 345                           

gct gac ggt ttc tct aag atc cca gct atg atc gct cca acc ttg aga       1106
Ala Asp Gly Phe Ser Lys Ile Pro Ala Met Ile Ala Pro Thr Leu Arg           
350                 355                 360                 365           

gag tgt tac cag aga ggt gtt aga cca aac gct acc gct atg ttg cca       1154
Glu Cys Tyr Gln Arg Gly Val Arg Pro Asn Ala Thr Ala Met Leu Pro           
                370                 375                 380               

gct ttg ttc tac gtt ttc atg gag cag tgg cac cac ggt aag ttg cca       1202
Ala Leu Phe Tyr Val Phe Met Glu Gln Trp His His Gly Lys Leu Pro           
            385                 390                 395                   

tac gag tac cag gac ggt atc ttg gac gct cca gct gtt cac gct atg       1250
Tyr Glu Tyr Gln Asp Gly Ile Leu Asp Ala Pro Ala Val His Ala Met           
        400                 405                 410                       

ttg cag tct gct gac cca gtt gct gtt tac gct tct gac aag gct ttg       1298
Leu Gln Ser Ala Asp Pro Val Ala Val Tyr Ala Ser Asp Lys Ala Leu           
    415                 420                 425                           

ttc ggt gac ttg acc gag aga gag gac ttc gct gct ttg ttg aga gag       1346
Phe Gly Asp Leu Thr Glu Arg Glu Asp Phe Ala Ala Leu Leu Arg Glu           
430                 435                 440                 445           

aag atc gct gac gtt tac gct ttg atc aac taa gcatgc                    1385
Lys Ile Ala Asp Val Tyr Ala Leu Ile Asn                                   
                450                 455                                   


<210>  2
<211>  455
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Asn Glu Gln Phe Thr Trp Leu His Ile Gly Leu Gly Ser Phe His 
1               5                   10                  15      


Arg Ala His Gln Ala Trp Tyr Leu His Arg Leu Gln Val Met Gly Asp 
            20                  25                  30          


Lys Arg Trp Ser Ile Ala Ala Gly Asn Ile Arg Asn Asp Ala Glu His 
        35                  40                  45              


Val Val Gln Ala Leu Ser Ala Gln Lys Gly Arg Tyr Val Leu Glu Thr 
    50                  55                  60                  


Val Ser Pro Glu Gly Val Ser Glu Tyr Glu Glu Ile Thr Ser Ile Gln 
65                  70                  75                  80  


Lys Leu Ile Pro Trp Gln Ala Asp Leu Gln Pro Leu Ile Ala Glu Gly 
                85                  90                  95      


Ala Asp Pro Lys Thr Lys Val Ile Ala Phe Thr Val Thr Glu Gly Gly 
            100                 105                 110         


Tyr Tyr Leu Asn Thr Ser His Lys Leu Glu Val Asn Asn Pro Asp Leu 
        115                 120                 125             


Ala Ala Asp Leu Lys Gly Gly Cys Lys Thr Ile Tyr Gly Val Ile Thr 
    130                 135                 140                 


Arg Ile Leu Glu Ala Arg Met Ala Asn Asn Ala Gly Pro Leu Thr Leu 
145                 150                 155                 160 


Leu Asn Cys Asp Asn Val Arg His Asn Gly Glu Arg Phe His Asp Gly 
                165                 170                 175     


Leu Val Glu Phe Leu Gln Leu Thr Gly Lys Gln Asp Val Ile Asp Trp 
            180                 185                 190         


Leu Ser Thr Asn Thr Thr Cys Pro Asn Thr Met Val Asp Arg Ile Thr 
        195                 200                 205             


Pro Arg Pro Ala Ala Glu Leu Pro Ala Arg Ile Lys Ala Gln Thr Gly 
    210                 215                 220                 


Ile Ala Asp Lys Ala Pro Val Met Gly Glu Thr Phe Ile Gln Trp Val 
225                 230                 235                 240 


Val Glu Asp Asn Phe Arg Asp Val Arg Pro Ala Leu Glu Lys Val Gly 
                245                 250                 255     


Val Glu Leu Val Ala Ser Val Ile Pro Tyr Glu Glu Ala Lys Ile Arg 
            260                 265                 270         


Ile Leu Asn Ser Ser His Ser Cys Ile Ala Trp Ala Gly Thr Leu Ile 
        275                 280                 285             


Gly Gln Lys Tyr Ile His Glu Ser Thr Met Thr Asp Phe Ile Tyr Gln 
    290                 295                 300                 


Ile Ala Asp Arg Tyr Val Thr Glu Asp Val Ile Pro Cys Leu Gly Asp 
305                 310                 315                 320 


Asn Gly Ile Asp Leu Pro Thr Tyr Arg Asp Val Val Leu Lys Arg Phe 
                325                 330                 335     


Thr Asn Pro His Ile Gln Asp Thr Asn Gln Arg Val Ala Ala Asp Gly 
            340                 345                 350         


Phe Ser Lys Ile Pro Ala Met Ile Ala Pro Thr Leu Arg Glu Cys Tyr 
        355                 360                 365             


Gln Arg Gly Val Arg Pro Asn Ala Thr Ala Met Leu Pro Ala Leu Phe 
    370                 375                 380                 


Tyr Val Phe Met Glu Gln Trp His His Gly Lys Leu Pro Tyr Glu Tyr 
385                 390                 395                 400 


Gln Asp Gly Ile Leu Asp Ala Pro Ala Val His Ala Met Leu Gln Ser 
                405                 410                 415     


Ala Asp Pro Val Ala Val Tyr Ala Ser Asp Lys Ala Leu Phe Gly Asp 
            420                 425                 430         


Leu Thr Glu Arg Glu Asp Phe Ala Ala Leu Leu Arg Glu Lys Ile Ala 
        435                 440                 445             


Asp Val Tyr Ala Leu Ile Asn 
    450                 455 


<210>  3
<211>  1368
<212>  DNA
<213>  artificial sequence

<220>
<223>  NAD+ specific D-arabitol 4-oxidoreductase encoding sequence

<400>  3
atgaacgagc agttcacctg gttgcacatc ggtttgggtt ctttccacag agctcaccag       60

gcttggtact tgcacagatt gcaggttatg ggtgacaaga gatggtctat cgctgctggt      120

aacatcagaa acgacgctga gcacgttgtt caggctttgt ctgctcagaa gggtagatac      180

gttttggaga ccgtttctcc agagggtgtt tctgagtacg aggagatcac ctctatccag      240

aagttgatcc catggcaggc tgacttgcag ccattgatcg ctgagggtgc tgacccaaag      300

accaaggtta tcgctttcac cgttaccgag ggtggttact acttgaacac ctctcacaag      360

ttggaggtta acaacccaga cttggctgct gacttgaagg gtggttgtaa gaccatctac      420

ggtgttatca ccagaatctt ggaggctaga atggctaaca acgctggtcc attgaccttg      480

ttgaactgtg acaacgttag acacaacggt gagagattcc acgacggttt ggttgagttc      540

ttgcagttga ccggtaagca ggacgttatc gactggttgt ctaccaacac cacctgtcca      600

aacaccatgg ttgacagaat caccccaaga ccagctgctg agttgccagc tagaatcaag      660

gctcagaccg gtatcgctga caaggctcca gttatgggtg agaccttcat ccagtgggtt      720

gttgaggaca acttcagaga cgttagacca gctttggaga aggttggtgt tgagttggtt      780

gcttctgtta tcccatacga ggaggctaag atcagaatct tgaactcttc tcactcttgt      840

atcgcttggg ctggtacctt gatcggtcag aagtacatcc acgagtctac catgaccgac      900

ttcatctacc agatcgctga cagatacgtt accgaggacg ttatcccatg tttgggtgac      960

aacggtatcg acttgccaac ctacagagac gttgttttga agagattcac caacccacac     1020

atccaggaca ccaaccagag agttgctgct gacggtttct ctaagatccc agctatgatc     1080

gctccaacct tgagagagtg ttaccagaga ggtgttagac caaacgctac cgctatgttg     1140

ccagctttgt tctacgtttt catggagcag tggcaccacg gtaagttgcc atacgagtac     1200

caggacggta tcttggacgc tccagctgtt cacgctatgt tgcagtctgc tgacccagtt     1260

gctgtttacg cttctgacaa ggctttgttc ggtgacttga ccgagagaga ggacttcgct     1320

gctttgttga gagagaagat cgctgacgtt tacgctttga tcaactaa                  1368


<210>  4
<211>  1107
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence encoding NADPH-specific xylitol dehydrogenase flanked by
       restriction sites


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  HindIII recognition site

<220>
<221>  CDS
<222>  (10)..(1101)

<220>
<221>  misc_feature
<222>  (1102)..(1107)
<223>  SacII recognition site

<400>  4
aagcttaaa atg act gct aac cct tcc ttg gtg ttg aac aag atc gac gac       51
          Met Thr Ala Asn Pro Ser Leu Val Leu Asn Lys Ile Asp Asp         
          1               5                   10                          

att tcg ttc gaa act tac gat gcc cca gaa atc tct gaa cct acc gat         99
Ile Ser Phe Glu Thr Tyr Asp Ala Pro Glu Ile Ser Glu Pro Thr Asp           
15                  20                  25                  30            

gtc ctc gtc cag gtc aag aaa acc ggt atc tgt ggt tcc gac atc cac        147
Val Leu Val Gln Val Lys Lys Thr Gly Ile Cys Gly Ser Asp Ile His           
                35                  40                  45                

ttc tac gcc cat ggt aga atc ggt aac ttc gtt ttg acc aag cca atg        195
Phe Tyr Ala His Gly Arg Ile Gly Asn Phe Val Leu Thr Lys Pro Met           
            50                  55                  60                    

gtc ttg ggt cac gaa tcc gcc ggt act gtt gtc cag gtt ggt aag ggt        243
Val Leu Gly His Glu Ser Ala Gly Thr Val Val Gln Val Gly Lys Gly           
        65                  70                  75                        

gtc acc tct ctt aag gtt ggt gac aac gtc gct atc gaa cca ggt att        291
Val Thr Ser Leu Lys Val Gly Asp Asn Val Ala Ile Glu Pro Gly Ile           
    80                  85                  90                            

cca tcc aga ttc tcc gac gaa tac aag agc ggt cac tac aac ttg tgt        339
Pro Ser Arg Phe Ser Asp Glu Tyr Lys Ser Gly His Tyr Asn Leu Cys           
95                  100                 105                 110           

cct cac atg gcc ttc gcc gct act cct aac tcc aag gaa ggc gaa cca        387
Pro His Met Ala Phe Ala Ala Thr Pro Asn Ser Lys Glu Gly Glu Pro           
                115                 120                 125               

aac cca cca ggt acc tta tgt aag tac ttc aag tcg cca gaa gac ttc        435
Asn Pro Pro Gly Thr Leu Cys Lys Tyr Phe Lys Ser Pro Glu Asp Phe           
            130                 135                 140                   

ttg gtc aag ttg cca gac cac gtc agc ttg gaa ctc ggt gct ctt gtt        483
Leu Val Lys Leu Pro Asp His Val Ser Leu Glu Leu Gly Ala Leu Val           
        145                 150                 155                       

gag cca ttg tct gtt ggt gtc cac gcc tcc aag ttg ggt tcc gtt gct        531
Glu Pro Leu Ser Val Gly Val His Ala Ser Lys Leu Gly Ser Val Ala           
    160                 165                 170                           

ttc ggc gac tac gtt gcc gtc ttt ggt gct ggt cct gtt ggt ctt ttg        579
Phe Gly Asp Tyr Val Ala Val Phe Gly Ala Gly Pro Val Gly Leu Leu           
175                 180                 185                 190           

gct gct gct gtc gcc aag acc ttc ggt gct aag ggt gtc atc gtc gtt        627
Ala Ala Ala Val Ala Lys Thr Phe Gly Ala Lys Gly Val Ile Val Val           
                195                 200                 205               

gct aga tca gac aga aag ttg aag atg gcc aag gac att ggt gct gct        675
Ala Arg Ser Asp Arg Lys Leu Lys Met Ala Lys Asp Ile Gly Ala Ala           
            210                 215                 220                   

act cac acc ttc aac tcc aag acc ggt ggt tct gaa gaa ttg atc aag        723
Thr His Thr Phe Asn Ser Lys Thr Gly Gly Ser Glu Glu Leu Ile Lys           
        225                 230                 235                       

gct ttc ggt ggt aac gtg cca aac gtc gtt ttg gaa tgt act ggt gct        771
Ala Phe Gly Gly Asn Val Pro Asn Val Val Leu Glu Cys Thr Gly Ala           
    240                 245                 250                           

gaa cct tgt atc aag ttg ggt gtt gac gcc att gcc cca ggt ggt cgt        819
Glu Pro Cys Ile Lys Leu Gly Val Asp Ala Ile Ala Pro Gly Gly Arg           
255                 260                 265                 270           

ttc gtt caa gtt ggt aac gct gct ggt cca gtc agc ttc cca atc acc        867
Phe Val Gln Val Gly Asn Ala Ala Gly Pro Val Ser Phe Pro Ile Thr           
                275                 280                 285               

gtt ttc gcc atg aag gaa ttg act ttg ttc ggt tct ttc aga tac gga        915
Val Phe Ala Met Lys Glu Leu Thr Leu Phe Gly Ser Phe Arg Tyr Gly           
            290                 295                 300                   

ttc aac gac tac aag act gct gtt gga atc ttt gac act aac tac caa        963
Phe Asn Asp Tyr Lys Thr Ala Val Gly Ile Phe Asp Thr Asn Tyr Gln           
        305                 310                 315                       

aac ggt aga gaa aat gct cca att gac ttt gaa caa ttg atc acc cac       1011
Asn Gly Arg Glu Asn Ala Pro Ile Asp Phe Glu Gln Leu Ile Thr His           
    320                 325                 330                           

aga tac aag ttc aag gac gct att gaa gcc tac gac ttg gtc aga gcc       1059
Arg Tyr Lys Phe Lys Asp Ala Ile Glu Ala Tyr Asp Leu Val Arg Ala           
335                 340                 345                 350           

ggt aag ggt gct gtc aag tgt ctc att gac ggc cct gag taa ccgcgg        1107
Gly Lys Gly Ala Val Lys Cys Leu Ile Asp Gly Pro Glu                       
                355                 360                                   


<210>  5
<211>  363
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  5

Met Thr Ala Asn Pro Ser Leu Val Leu Asn Lys Ile Asp Asp Ile Ser 
1               5                   10                  15      


Phe Glu Thr Tyr Asp Ala Pro Glu Ile Ser Glu Pro Thr Asp Val Leu 
            20                  25                  30          


Val Gln Val Lys Lys Thr Gly Ile Cys Gly Ser Asp Ile His Phe Tyr 
        35                  40                  45              


Ala His Gly Arg Ile Gly Asn Phe Val Leu Thr Lys Pro Met Val Leu 
    50                  55                  60                  


Gly His Glu Ser Ala Gly Thr Val Val Gln Val Gly Lys Gly Val Thr 
65                  70                  75                  80  


Ser Leu Lys Val Gly Asp Asn Val Ala Ile Glu Pro Gly Ile Pro Ser 
                85                  90                  95      


Arg Phe Ser Asp Glu Tyr Lys Ser Gly His Tyr Asn Leu Cys Pro His 
            100                 105                 110         


Met Ala Phe Ala Ala Thr Pro Asn Ser Lys Glu Gly Glu Pro Asn Pro 
        115                 120                 125             


Pro Gly Thr Leu Cys Lys Tyr Phe Lys Ser Pro Glu Asp Phe Leu Val 
    130                 135                 140                 


Lys Leu Pro Asp His Val Ser Leu Glu Leu Gly Ala Leu Val Glu Pro 
145                 150                 155                 160 


Leu Ser Val Gly Val His Ala Ser Lys Leu Gly Ser Val Ala Phe Gly 
                165                 170                 175     


Asp Tyr Val Ala Val Phe Gly Ala Gly Pro Val Gly Leu Leu Ala Ala 
            180                 185                 190         


Ala Val Ala Lys Thr Phe Gly Ala Lys Gly Val Ile Val Val Ala Arg 
        195                 200                 205             


Ser Asp Arg Lys Leu Lys Met Ala Lys Asp Ile Gly Ala Ala Thr His 
    210                 215                 220                 


Thr Phe Asn Ser Lys Thr Gly Gly Ser Glu Glu Leu Ile Lys Ala Phe 
225                 230                 235                 240 


Gly Gly Asn Val Pro Asn Val Val Leu Glu Cys Thr Gly Ala Glu Pro 
                245                 250                 255     


Cys Ile Lys Leu Gly Val Asp Ala Ile Ala Pro Gly Gly Arg Phe Val 
            260                 265                 270         


Gln Val Gly Asn Ala Ala Gly Pro Val Ser Phe Pro Ile Thr Val Phe 
        275                 280                 285             


Ala Met Lys Glu Leu Thr Leu Phe Gly Ser Phe Arg Tyr Gly Phe Asn 
    290                 295                 300                 


Asp Tyr Lys Thr Ala Val Gly Ile Phe Asp Thr Asn Tyr Gln Asn Gly 
305                 310                 315                 320 


Arg Glu Asn Ala Pro Ile Asp Phe Glu Gln Leu Ile Thr His Arg Tyr 
                325                 330                 335     


Lys Phe Lys Asp Ala Ile Glu Ala Tyr Asp Leu Val Arg Ala Gly Lys 
            340                 345                 350         


Gly Ala Val Lys Cys Leu Ile Asp Gly Pro Glu 
        355                 360             


<210>  6
<211>  1092
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding NADPH specific xylitol dehydrogenase

<400>  6
atgactgcta acccttcctt ggtgttgaac aagatcgacg acatttcgtt cgaaacttac       60

gatgccccag aaatctctga acctaccgat gtcctcgtcc aggtcaagaa aaccggtatc      120

tgtggttccg acatccactt ctacgcccat ggtagaatcg gtaacttcgt tttgaccaag      180

ccaatggtct tgggtcacga atccgccggt actgttgtcc aggttggtaa gggtgtcacc      240

tctcttaagg ttggtgacaa cgtcgctatc gaaccaggta ttccatccag attctccgac      300

gaatacaaga gcggtcacta caacttgtgt cctcacatgg ccttcgccgc tactcctaac      360

tccaaggaag gcgaaccaaa cccaccaggt accttatgta agtacttcaa gtcgccagaa      420

gacttcttgg tcaagttgcc agaccacgtc agcttggaac tcggtgctct tgttgagcca      480

ttgtctgttg gtgtccacgc ctccaagttg ggttccgttg ctttcggcga ctacgttgcc      540

gtctttggtg ctggtcctgt tggtcttttg gctgctgctg tcgccaagac cttcggtgct      600

aagggtgtca tcgtcgttgc tagatcagac agaaagttga agatggccaa ggacattggt      660

gctgctactc acaccttcaa ctccaagacc ggtggttctg aagaattgat caaggctttc      720

ggtggtaacg tgccaaacgt cgttttggaa tgtactggtg ctgaaccttg tatcaagttg      780

ggtgttgacg ccattgcccc aggtggtcgt ttcgttcaag ttggtaacgc tgctggtcca      840

gtcagcttcc caatcaccgt tttcgccatg aaggaattga ctttgttcgg ttctttcaga      900

tacggattca acgactacaa gactgctgtt ggaatctttg acactaacta ccaaaacggt      960

agagaaaatg ctccaattga ctttgaacaa ttgatcaccc acagatacaa gttcaaggac     1020

gctattgaag cctacgactt ggtcagagcc ggtaagggtg ctgtcaagtg tctcattgac     1080

ggccctgagt aa                                                         1092


<210>  7
<211>  806
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding NADPH-specific xylitol dehydrogenase flanked by
       restriction sites


<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  AscI recognition site

<220>
<221>  CDS
<222>  (12)..(800)

<220>
<221>  misc_feature
<222>  (801)..(806)
<223>  SphI recognition site

<400>  7
ggcgcgccaa a atg tct aag aag ttc aac ggt aag gtt tgt ttg gtt acc        50
             Met Ser Lys Lys Phe Asn Gly Lys Val Cys Leu Val Thr          
             1               5                   10                       

ggt gct ggt ggt aac atc ggt ttg gct acc gct ttg aga ttg gct gag         98
Gly Ala Gly Gly Asn Ile Gly Leu Ala Thr Ala Leu Arg Leu Ala Glu           
    15                  20                  25                            

gag ggt acc gct atc gct ttg ttg tct aga aac aga gag gct ttg gag        146
Glu Gly Thr Ala Ile Ala Leu Leu Ser Arg Asn Arg Glu Ala Leu Glu           
30                  35                  40                  45            

aag gct gag gct tct gtt aga gag aag ggt gtt gag gct aga tct tac        194
Lys Ala Glu Ala Ser Val Arg Glu Lys Gly Val Glu Ala Arg Ser Tyr           
                50                  55                  60                

gtt tgt gac gtt acc tct gag gag gct gtt atc ggt acc gtt gac tct        242
Val Cys Asp Val Thr Ser Glu Glu Ala Val Ile Gly Thr Val Asp Ser           
            65                  70                  75                    

gtt gtt aga gac ttc ggt aag atc gac ttc ttg ttc aac aac gct ggt        290
Val Val Arg Asp Phe Gly Lys Ile Asp Phe Leu Phe Asn Asn Ala Gly           
        80                  85                  90                        

tac cag ggt gct ttc gct cca gtt cag gac tac cca tct gac gac ttc        338
Tyr Gln Gly Ala Phe Ala Pro Val Gln Asp Tyr Pro Ser Asp Asp Phe           
    95                  100                 105                           

gct aga gtt ttg acc atc aac gtt acc ggt gct ttc cac gtt ttg aag        386
Ala Arg Val Leu Thr Ile Asn Val Thr Gly Ala Phe His Val Leu Lys           
110                 115                 120                 125           

gct gtt tct aga cag atg atc acc cag aac tac ggt aga atc gtt aac        434
Ala Val Ser Arg Gln Met Ile Thr Gln Asn Tyr Gly Arg Ile Val Asn           
                130                 135                 140               

acc gct tct atg gct ggt gtt aag ggt cca cca aac atg gct gct tac        482
Thr Ala Ser Met Ala Gly Val Lys Gly Pro Pro Asn Met Ala Ala Tyr           
            145                 150                 155                   

ggt acc tct aag ggt gct atc atc gct ttg acc gag acc gct gct ttg        530
Gly Thr Ser Lys Gly Ala Ile Ile Ala Leu Thr Glu Thr Ala Ala Leu           
        160                 165                 170                       

gac ttg gct cca tac aac atc aga gtt aac gct atc tct cca ggt tac        578
Asp Leu Ala Pro Tyr Asn Ile Arg Val Asn Ala Ile Ser Pro Gly Tyr           
    175                 180                 185                           

atg ggt cca ggt ttc atg tgg gag aga cag gtt gag ttg cag gct aag        626
Met Gly Pro Gly Phe Met Trp Glu Arg Gln Val Glu Leu Gln Ala Lys           
190                 195                 200                 205           

gtt ggt tct cag tac ttc tct acc gac cca aag gtt gtt gct cag cag        674
Val Gly Ser Gln Tyr Phe Ser Thr Asp Pro Lys Val Val Ala Gln Gln           
                210                 215                 220               

atg atc ggt tct gtt cca atg aga aga tac ggt gac atc aac gag atc        722
Met Ile Gly Ser Val Pro Met Arg Arg Tyr Gly Asp Ile Asn Glu Ile           
            225                 230                 235                   

cca ggt gtt gtt gct ttc ttg ttg ggt gac gac tct tct ttc atg acc        770
Pro Gly Val Val Ala Phe Leu Leu Gly Asp Asp Ser Ser Phe Met Thr           
        240                 245                 250                       

ggt gtt aac ttg cca atc gct ggt ggt tga gcatgc                         806
Gly Val Asn Leu Pro Ile Ala Gly Gly                                       
    255                 260                                               


<210>  8
<211>  262
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Ser Lys Lys Phe Asn Gly Lys Val Cys Leu Val Thr Gly Ala Gly 
1               5                   10                  15      


Gly Asn Ile Gly Leu Ala Thr Ala Leu Arg Leu Ala Glu Glu Gly Thr 
            20                  25                  30          


Ala Ile Ala Leu Leu Ser Arg Asn Arg Glu Ala Leu Glu Lys Ala Glu 
        35                  40                  45              


Ala Ser Val Arg Glu Lys Gly Val Glu Ala Arg Ser Tyr Val Cys Asp 
    50                  55                  60                  


Val Thr Ser Glu Glu Ala Val Ile Gly Thr Val Asp Ser Val Val Arg 
65                  70                  75                  80  


Asp Phe Gly Lys Ile Asp Phe Leu Phe Asn Asn Ala Gly Tyr Gln Gly 
                85                  90                  95      


Ala Phe Ala Pro Val Gln Asp Tyr Pro Ser Asp Asp Phe Ala Arg Val 
            100                 105                 110         


Leu Thr Ile Asn Val Thr Gly Ala Phe His Val Leu Lys Ala Val Ser 
        115                 120                 125             


Arg Gln Met Ile Thr Gln Asn Tyr Gly Arg Ile Val Asn Thr Ala Ser 
    130                 135                 140                 


Met Ala Gly Val Lys Gly Pro Pro Asn Met Ala Ala Tyr Gly Thr Ser 
145                 150                 155                 160 


Lys Gly Ala Ile Ile Ala Leu Thr Glu Thr Ala Ala Leu Asp Leu Ala 
                165                 170                 175     


Pro Tyr Asn Ile Arg Val Asn Ala Ile Ser Pro Gly Tyr Met Gly Pro 
            180                 185                 190         


Gly Phe Met Trp Glu Arg Gln Val Glu Leu Gln Ala Lys Val Gly Ser 
        195                 200                 205             


Gln Tyr Phe Ser Thr Asp Pro Lys Val Val Ala Gln Gln Met Ile Gly 
    210                 215                 220                 


Ser Val Pro Met Arg Arg Tyr Gly Asp Ile Asn Glu Ile Pro Gly Val 
225                 230                 235                 240 


Val Ala Phe Leu Leu Gly Asp Asp Ser Ser Phe Met Thr Gly Val Asn 
                245                 250                 255     


Leu Pro Ile Ala Gly Gly 
            260         


<210>  9
<211>  789
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding NADPH specific xylitol dehydrogenase

<400>  9
atgtctaaga agttcaacgg taaggtttgt ttggttaccg gtgctggtgg taacatcggt       60

ttggctaccg ctttgagatt ggctgaggag ggtaccgcta tcgctttgtt gtctagaaac      120

agagaggctt tggagaaggc tgaggcttct gttagagaga agggtgttga ggctagatct      180

tacgtttgtg acgttacctc tgaggaggct gttatcggta ccgttgactc tgttgttaga      240

gacttcggta agatcgactt cttgttcaac aacgctggtt accagggtgc tttcgctcca      300

gttcaggact acccatctga cgacttcgct agagttttga ccatcaacgt taccggtgct      360

ttccacgttt tgaaggctgt ttctagacag atgatcaccc agaactacgg tagaatcgtt      420

aacaccgctt ctatggctgg tgttaagggt ccaccaaaca tggctgctta cggtacctct      480

aagggtgcta tcatcgcttt gaccgagacc gctgctttgg acttggctcc atacaacatc      540

agagttaacg ctatctctcc aggttacatg ggtccaggtt tcatgtggga gagacaggtt      600

gagttgcagg ctaaggttgg ttctcagtac ttctctaccg acccaaaggt tgttgctcag      660

cagatgatcg gttctgttcc aatgagaaga tacggtgaca tcaacgagat cccaggtgtt      720

gttgctttct tgttgggtga cgactcttct ttcatgaccg gtgttaactt gccaatcgct      780

ggtggttga                                                              789


<210>  10
<211>  873
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding tagatose-3-epimerase


<220>
<221>  CDS
<222>  (1)..(873)

<400>  10
atg aac aaa gtt ggc atg ttc tac acc tac tgg tcg act gag tgg atg         48
Met Asn Lys Val Gly Met Phe Tyr Thr Tyr Trp Ser Thr Glu Trp Met           
1               5                   10                  15                

gtc gac ttt ccg gcg act gcg aag cgc att gcc ggg ctc ggc ttc gac         96
Val Asp Phe Pro Ala Thr Ala Lys Arg Ile Ala Gly Leu Gly Phe Asp           
            20                  25                  30                    

tta atg gaa atc tcg ctc ggc gag ttt cac aat ctt tcc gac gcg aag        144
Leu Met Glu Ile Ser Leu Gly Glu Phe His Asn Leu Ser Asp Ala Lys           
        35                  40                  45                        

aag cgt gag cta aaa gcc gtg gct gat gat ctg ggg ctc acg gtg atg        192
Lys Arg Glu Leu Lys Ala Val Ala Asp Asp Leu Gly Leu Thr Val Met           
    50                  55                  60                            

tgc tgt atc gga ctg aag tct gag tac gac ttt gcc tcg ccg gac aag        240
Cys Cys Ile Gly Leu Lys Ser Glu Tyr Asp Phe Ala Ser Pro Asp Lys           
65                  70                  75                  80            

agc gtt cgt gat gcc ggc acg gaa tat gtg aag cgc ttg ctc gac gac        288
Ser Val Arg Asp Ala Gly Thr Glu Tyr Val Lys Arg Leu Leu Asp Asp           
                85                  90                  95                

tgt cac ctc ctc ggt gcg ccg gtc ttt gct ggc ctt acg ttc tgc gcg        336
Cys His Leu Leu Gly Ala Pro Val Phe Ala Gly Leu Thr Phe Cys Ala           
            100                 105                 110                   

tgg ccc caa tct ccg ccg ctg gac atg aag gat aag cgc cct tac gtc        384
Trp Pro Gln Ser Pro Pro Leu Asp Met Lys Asp Lys Arg Pro Tyr Val           
        115                 120                 125                       

gac cgt gca atc gaa agc gtt cgt cgt gtt atc aag gta gct gaa gac        432
Asp Arg Ala Ile Glu Ser Val Arg Arg Val Ile Lys Val Ala Glu Asp           
    130                 135                 140                           

tac ggc att att tat gca ctg gaa gtg gtg aac cga ttc gag cag tgg        480
Tyr Gly Ile Ile Tyr Ala Leu Glu Val Val Asn Arg Phe Glu Gln Trp           
145                 150                 155                 160           

ctt tgc aat gac gcc aag gaa gca att gcg ttt gcc gac gcg gtt gac        528
Leu Cys Asn Asp Ala Lys Glu Ala Ile Ala Phe Ala Asp Ala Val Asp           
                165                 170                 175               

agt ccg gcg tgc aag gtc cag ctc gac aca ttc cac atg aat atc gaa        576
Ser Pro Ala Cys Lys Val Gln Leu Asp Thr Phe His Met Asn Ile Glu           
            180                 185                 190                   

gag act tcc ttc cgc gat gca atc ctt gcc tgc aag ggc aag atg ggc        624
Glu Thr Ser Phe Arg Asp Ala Ile Leu Ala Cys Lys Gly Lys Met Gly           
        195                 200                 205                       

cat ttc cat ttg ggc gaa gcg aac cgt ctg ccg ccg ggc gag ggt cgc        672
His Phe His Leu Gly Glu Ala Asn Arg Leu Pro Pro Gly Glu Gly Arg           
    210                 215                 220                           

ctg ccg tgg gat gaa ata ttc ggg gcg ctg aag gaa atc gga tat gac        720
Leu Pro Trp Asp Glu Ile Phe Gly Ala Leu Lys Glu Ile Gly Tyr Asp           
225                 230                 235                 240           

ggc acc atc gtt atg gaa ccg ttc atg cgc aag ggc ggc tcg gtc agc        768
Gly Thr Ile Val Met Glu Pro Phe Met Arg Lys Gly Gly Ser Val Ser           
                245                 250                 255               

cgc gcg gtg ggc gta tgg cgg gat atg tcg aac ggt gcg acg gac gaa        816
Arg Ala Val Gly Val Trp Arg Asp Met Ser Asn Gly Ala Thr Asp Glu           
            260                 265                 270                   

gag atg gac gag cgc gct cgc cgc tcg ttg cag ttt gtt cgt gac aag        864
Glu Met Asp Glu Arg Ala Arg Arg Ser Leu Gln Phe Val Arg Asp Lys           
        275                 280                 285                       

ctg gcc tga                                                            873
Leu Ala                                                                   
    290                                                                   


<210>  11
<211>  290
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  11

Met Asn Lys Val Gly Met Phe Tyr Thr Tyr Trp Ser Thr Glu Trp Met 
1               5                   10                  15      


Val Asp Phe Pro Ala Thr Ala Lys Arg Ile Ala Gly Leu Gly Phe Asp 
            20                  25                  30          


Leu Met Glu Ile Ser Leu Gly Glu Phe His Asn Leu Ser Asp Ala Lys 
        35                  40                  45              


Lys Arg Glu Leu Lys Ala Val Ala Asp Asp Leu Gly Leu Thr Val Met 
    50                  55                  60                  


Cys Cys Ile Gly Leu Lys Ser Glu Tyr Asp Phe Ala Ser Pro Asp Lys 
65                  70                  75                  80  


Ser Val Arg Asp Ala Gly Thr Glu Tyr Val Lys Arg Leu Leu Asp Asp 
                85                  90                  95      


Cys His Leu Leu Gly Ala Pro Val Phe Ala Gly Leu Thr Phe Cys Ala 
            100                 105                 110         


Trp Pro Gln Ser Pro Pro Leu Asp Met Lys Asp Lys Arg Pro Tyr Val 
        115                 120                 125             


Asp Arg Ala Ile Glu Ser Val Arg Arg Val Ile Lys Val Ala Glu Asp 
    130                 135                 140                 


Tyr Gly Ile Ile Tyr Ala Leu Glu Val Val Asn Arg Phe Glu Gln Trp 
145                 150                 155                 160 


Leu Cys Asn Asp Ala Lys Glu Ala Ile Ala Phe Ala Asp Ala Val Asp 
                165                 170                 175     


Ser Pro Ala Cys Lys Val Gln Leu Asp Thr Phe His Met Asn Ile Glu 
            180                 185                 190         


Glu Thr Ser Phe Arg Asp Ala Ile Leu Ala Cys Lys Gly Lys Met Gly 
        195                 200                 205             


His Phe His Leu Gly Glu Ala Asn Arg Leu Pro Pro Gly Glu Gly Arg 
    210                 215                 220                 


Leu Pro Trp Asp Glu Ile Phe Gly Ala Leu Lys Glu Ile Gly Tyr Asp 
225                 230                 235                 240 


Gly Thr Ile Val Met Glu Pro Phe Met Arg Lys Gly Gly Ser Val Ser 
                245                 250                 255     


Arg Ala Val Gly Val Trp Arg Asp Met Ser Asn Gly Ala Thr Asp Glu 
            260                 265                 270         


Glu Met Asp Glu Arg Ala Arg Arg Ser Leu Gln Phe Val Arg Asp Lys 
        275                 280                 285             


Leu Ala 
    290 


<210>  12
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  12
gaactagtgg atccgtagaa atcttg                                            26


<210>  13
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  13
ctttgttcat tttggcgcgc cttttagttt aataagggtc cgtg                        44


<210>  14
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  14
aaactaaaag gcgcgccaaa atgaacaaag ttggcatg                               38


<210>  15
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  15
ttctcttcga gagcatgctc aggccagctt gtcacg                                 36


<210>  16
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  16
aagctggcct gagcatgctc tcgaagagaa tctag                                  35


<210>  17
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  17
gttccgcgga gaatgacacg gccgac                                            26


<210>  18
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  18
aaggcgcgcc aaaatgactg ctaacccttc c                                      31


<210>  19
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  19
gagcatgctt actcagggcc gtcaatg                                           27


<210>  20
<211>  22
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  20
gaagactagt tcacgtgatc tc                                                22


<210>  21
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  21
cactggcgcg ccttttgtgt ggtggtgtcc                                        30


<210>  22
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  22
tagcagcatg cataggttag tgaatgaggt atg                                    33


<210>  23
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  23
taggtccgcg ggagcttcgt taaagggc                                          28


<210>  24
<211>  116
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  24
cactggcgcg cccactgcat gcgtcgacaa cccttaatat aacttcgtat aatgtatgct       60

atacgaagtt attaggtcta gacacatcgt ggatccaagc tatcaacgag agagtc          116


<210>  25
<211>  105
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  25
agtggctagc agtgccatgg cctaataact tcgtatagca tacattatac gaagttatat       60

taagggttct cgagacgcgt catctagcat ctcatctacc aactc                      105


<210>  26
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  26
cactctgcag cactggcgcg cccactgcat                                        30


<210>  27
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  27
cactgatatc agtggctagc agtgccatgg                                        30


<210>  28
<211>  590
<212>  DNA
<213>  artificial sequence

<220>
<223>  sequence encoding the nat1 gene of Streptomyces noursei

<400>  28
ggcgcgccaa aatgggtacc actttggacg acactgctta cagatacaga acctctgtcc       60

caggtgacgc cgaggccatc gaggctttgg atggttcctt caccaccgac accgtcttca      120

gagtcaccgc caccggtgac ggtttcacct tgagagaggt tccagttgac ccacctttga      180

ccaaggtttt cccagacgac gaatctgacg acgaatctga cgacggtgag gacggtgacc      240

cagactccag aactttcgtc gcctacggtg acgacggtga cttggctggt ttcgttgtcg      300

tctcttactc cggttggaac agaagattga ccgtcgagga catcgaggtc gccccagagc      360

acagaggtca cggtgtcggt agagctttga tgggtttggc tactgagttc gccagagaga      420

gaggtgccgg tcacttgtgg ttggaggtca ccaacgtcaa cgctccagct atccacgctt      480

acagaagaat gggtttcacc ttgtgtggtt tggacaccgc cttgtacgac ggtaccgcct      540

ccgacggtga gcaggctttg tacatgtcca tgccatgtcc ataagcatgc                 590


<210>  29
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  29
caagcatgcg ggaatgataa gagactttg                                         29


<210>  30
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  30
ggaccgcgga aaggtgagga agtatatgaa c                                      31


<210>  31
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  31
cacttctaga cactatcgat ggatccgtag aaatcttg                               38


<210>  32
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  32
cactacgcgt aaaggtgagg aagtatatg                                         29


<210>  33
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  33
cactctgcag gatccaagct atcaacgaga                                        30


<210>  34
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  34
cactgcatgc gttgcggaaa aaacagcc                                          28


<210>  35
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  35
cactccatgg agtaggtata taaaaatata agag                                   34


<210>  36
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>   primer

<400>  36
cactgctagc gtcgacaaca gcaactag                                          28


<210>  37
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  37
caagtcgtgg agattctgc                                                    19


<210>  38
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  38
caaggcgcgc caaaatgtct accaaaacca ttac                                   34


<210>  39
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  39
ggagcatgcc tactttccct cagccaag                                          28


<210>  40
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>   primer

<400>  40
atatcccggg caccgtcatc accgaaacgc                                        30


<210>  41
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer

<400>  41
atatcccggg cacgaccacg ctgatgagc                                         29


<210>  42
<211>  1415
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence encoding the NAD+-specific D-arabitol 4-oxidoreductase 
       from R. solanacearum


<220>
<221>  CDS
<222>  (12)..(1409)

<400>  42
ggcgcgccaa a atg acc aga tct tct ttg gct aga gct cca gtt ttg ttg        50
             Met Thr Arg Ser Ser Leu Ala Arg Ala Pro Val Leu Leu          
             1               5                   10                       

cac atc ggt gct ggt tct ttc cac aga gct cac cag gct tgg tac ttg         98
His Ile Gly Ala Gly Ser Phe His Arg Ala His Gln Ala Trp Tyr Leu           
    15                  20                  25                            

cac aga gtt aac gct gct gtt cca cca ggt gag aga tgg acc ttg acc        146
His Arg Val Asn Ala Ala Val Pro Pro Gly Glu Arg Trp Thr Leu Thr           
30                  35                  40                  45            

gtt ggt aac atc aga gac gac atg cac gct acc ttg gct gct ttg gct        194
Val Gly Asn Ile Arg Asp Asp Met His Ala Thr Leu Ala Ala Leu Ala           
                50                  55                  60                

gct cag cag ggt gct tac acc ttg gag acc gtt acc cca cag ggt gag        242
Ala Gln Gln Gly Ala Tyr Thr Leu Glu Thr Val Thr Pro Gln Gly Glu           
            65                  70                  75                    

aga gct tac gag acc atc aga tct atc gct aga gtt ttg cct tgg tct        290
Arg Ala Tyr Glu Thr Ile Arg Ser Ile Ala Arg Val Leu Pro Trp Ser           
        80                  85                  90                        

gct gac ttg gct gct ttg atc aac acc ggt gct gac cca gct tgt aga        338
Ala Asp Leu Ala Ala Leu Ile Asn Thr Gly Ala Asp Pro Ala Cys Arg           
    95                  100                 105                           

atc gtt tct ttc acc gtt acc gag ggt ggt tac tac ttg gac gag cac        386
Ile Val Ser Phe Thr Val Thr Glu Gly Gly Tyr Tyr Leu Asp Glu His           
110                 115                 120                 125           

gac aga ttg gac gtt acc cac cca gac ttg gct gct gac ttg aga ggt        434
Asp Arg Leu Asp Val Thr His Pro Asp Leu Ala Ala Asp Leu Arg Gly           
                130                 135                 140               

gct aga tct acc ttg tac ggt gct ttg gct gct ttg ttg gct gag aga        482
Ala Arg Ser Thr Leu Tyr Gly Ala Leu Ala Ala Leu Leu Ala Glu Arg           
            145                 150                 155                   

aga cag aga ggt gct ggt cca ttg acc ttg cag tct tgt gac aac ttg        530
Arg Gln Arg Gly Ala Gly Pro Leu Thr Leu Gln Ser Cys Asp Asn Leu           
        160                 165                 170                       

aga tct aac ggt gct aga ttc aga gct ggt atg aga gct ttc ttg gct        578
Arg Ser Asn Gly Ala Arg Phe Arg Ala Gly Met Arg Ala Phe Leu Ala           
    175                 180                 185                           

ttg aga ggt gac gct gct ttg ttg gct tgg ttc gac gct aac gtt tct        626
Leu Arg Gly Asp Ala Ala Leu Leu Ala Trp Phe Asp Ala Asn Val Ser           
190                 195                 200                 205           

tgt cca tct gct atg gtt gac aga atc acc cca aga cca acc gac gac        674
Cys Pro Ser Ala Met Val Asp Arg Ile Thr Pro Arg Pro Thr Asp Asp           
                210                 215                 220               

gtt aga acc aga gtt cac gct gct acc ggt gtt gac gac aga tgt cca        722
Val Arg Thr Arg Val His Ala Ala Thr Gly Val Asp Asp Arg Cys Pro           
            225                 230                 235                   

gtt atg ggt gag tct ttc atc cag tgg gtt atc gag gac aac ttc atc        770
Val Met Gly Glu Ser Phe Ile Gln Trp Val Ile Glu Asp Asn Phe Ile           
        240                 245                 250                       

gct ggt aga cca gct tgg gag atc gct ggt gct gag atc gtt gct gac        818
Ala Gly Arg Pro Ala Trp Glu Ile Ala Gly Ala Glu Ile Val Ala Asp           
    255                 260                 265                           

gtt cac cca tac gag gag gct aag atc aga atc ttg aac gct acc cac        866
Val His Pro Tyr Glu Glu Ala Lys Ile Arg Ile Leu Asn Ala Thr His           
270                 275                 280                 285           

tct tgt atc gct tgg gct ggt acc ttg gct ggt ttg acc tac atc cac        914
Ser Cys Ile Ala Trp Ala Gly Thr Leu Ala Gly Leu Thr Tyr Ile His           
                290                 295                 300               

gag ggt atg aga gac gct gct atc tac aga ttc gct tac gac tac gtt        962
Glu Gly Met Arg Asp Ala Ala Ile Tyr Arg Phe Ala Tyr Asp Tyr Val           
            305                 310                 315                   

acc gac gac gtt atc cca tgt ttg acc cca tct cca ttg gac ttg gag       1010
Thr Asp Asp Val Ile Pro Cys Leu Thr Pro Ser Pro Leu Asp Leu Glu           
        320                 325                 330                       

aga tac aga gac gtt gtt ttg gag aga ttc ggt aac cca tac gtt ttg       1058
Arg Tyr Arg Asp Val Val Leu Glu Arg Phe Gly Asn Pro Tyr Val Leu           
    335                 340                 345                           

gac acc aac cag aga gtt gct gct gac ggt ttc tct aag atc cca ggt       1106
Asp Thr Asn Gln Arg Val Ala Ala Asp Gly Phe Ser Lys Ile Pro Gly           
350                 355                 360                 365           

ttc atc gct cca acc ttg gct gag tgt ttc gct aga ggt gct gac cca       1154
Phe Ile Ala Pro Thr Leu Ala Glu Cys Phe Ala Arg Gly Ala Asp Pro           
                370                 375                 380               

gtt gct acc gct gtt ttg cca gct ttg ttc ttg ggt ttc ttg gag ggt       1202
Val Ala Thr Ala Val Leu Pro Ala Leu Phe Leu Gly Phe Leu Glu Gly           
            385                 390                 395                   

tgg gct aga ggt acc ttg cca tac gtt tac cag gac ggt gtt atg gac       1250
Trp Ala Arg Gly Thr Leu Pro Tyr Val Tyr Gln Asp Gly Val Met Asp           
        400                 405                 410                       

ggt gct gct gct aga tct atc gtt gag gct cca gac tct gtt gct gct       1298
Gly Ala Ala Ala Arg Ser Ile Val Glu Ala Pro Asp Ser Val Ala Ala           
    415                 420                 425                           

ttc tgt tct gac aga cag ttg tgg ggt tct ttg gct ggt aga gac gct       1346
Phe Cys Ser Asp Arg Gln Leu Trp Gly Ser Leu Ala Gly Arg Asp Ala           
430                 435                 440                 445           

ttg gtt cag gct gtt aga gct ggt aga gct aga gtt gag gct tgg aga       1394
Leu Val Gln Ala Val Arg Ala Gly Arg Ala Arg Val Glu Ala Trp Arg           
                450                 455                 460               

gct gct aga aga tga gcatgc                                            1415
Ala Ala Arg Arg                                                           
            465                                                           


<210>  43
<211>  465
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  43

Met Thr Arg Ser Ser Leu Ala Arg Ala Pro Val Leu Leu His Ile Gly 
1               5                   10                  15      


Ala Gly Ser Phe His Arg Ala His Gln Ala Trp Tyr Leu His Arg Val 
            20                  25                  30          


Asn Ala Ala Val Pro Pro Gly Glu Arg Trp Thr Leu Thr Val Gly Asn 
        35                  40                  45              


Ile Arg Asp Asp Met His Ala Thr Leu Ala Ala Leu Ala Ala Gln Gln 
    50                  55                  60                  


Gly Ala Tyr Thr Leu Glu Thr Val Thr Pro Gln Gly Glu Arg Ala Tyr 
65                  70                  75                  80  


Glu Thr Ile Arg Ser Ile Ala Arg Val Leu Pro Trp Ser Ala Asp Leu 
                85                  90                  95      


Ala Ala Leu Ile Asn Thr Gly Ala Asp Pro Ala Cys Arg Ile Val Ser 
            100                 105                 110         


Phe Thr Val Thr Glu Gly Gly Tyr Tyr Leu Asp Glu His Asp Arg Leu 
        115                 120                 125             


Asp Val Thr His Pro Asp Leu Ala Ala Asp Leu Arg Gly Ala Arg Ser 
    130                 135                 140                 


Thr Leu Tyr Gly Ala Leu Ala Ala Leu Leu Ala Glu Arg Arg Gln Arg 
145                 150                 155                 160 


Gly Ala Gly Pro Leu Thr Leu Gln Ser Cys Asp Asn Leu Arg Ser Asn 
                165                 170                 175     


Gly Ala Arg Phe Arg Ala Gly Met Arg Ala Phe Leu Ala Leu Arg Gly 
            180                 185                 190         


Asp Ala Ala Leu Leu Ala Trp Phe Asp Ala Asn Val Ser Cys Pro Ser 
        195                 200                 205             


Ala Met Val Asp Arg Ile Thr Pro Arg Pro Thr Asp Asp Val Arg Thr 
    210                 215                 220                 


Arg Val His Ala Ala Thr Gly Val Asp Asp Arg Cys Pro Val Met Gly 
225                 230                 235                 240 


Glu Ser Phe Ile Gln Trp Val Ile Glu Asp Asn Phe Ile Ala Gly Arg 
                245                 250                 255     


Pro Ala Trp Glu Ile Ala Gly Ala Glu Ile Val Ala Asp Val His Pro 
            260                 265                 270         


Tyr Glu Glu Ala Lys Ile Arg Ile Leu Asn Ala Thr His Ser Cys Ile 
        275                 280                 285             


Ala Trp Ala Gly Thr Leu Ala Gly Leu Thr Tyr Ile His Glu Gly Met 
    290                 295                 300                 


Arg Asp Ala Ala Ile Tyr Arg Phe Ala Tyr Asp Tyr Val Thr Asp Asp 
305                 310                 315                 320 


Val Ile Pro Cys Leu Thr Pro Ser Pro Leu Asp Leu Glu Arg Tyr Arg 
                325                 330                 335     


Asp Val Val Leu Glu Arg Phe Gly Asn Pro Tyr Val Leu Asp Thr Asn 
            340                 345                 350         


Gln Arg Val Ala Ala Asp Gly Phe Ser Lys Ile Pro Gly Phe Ile Ala 
        355                 360                 365             


Pro Thr Leu Ala Glu Cys Phe Ala Arg Gly Ala Asp Pro Val Ala Thr 
    370                 375                 380                 


Ala Val Leu Pro Ala Leu Phe Leu Gly Phe Leu Glu Gly Trp Ala Arg 
385                 390                 395                 400 


Gly Thr Leu Pro Tyr Val Tyr Gln Asp Gly Val Met Asp Gly Ala Ala 
                405                 410                 415     


Ala Arg Ser Ile Val Glu Ala Pro Asp Ser Val Ala Ala Phe Cys Ser 
            420                 425                 430         


Asp Arg Gln Leu Trp Gly Ser Leu Ala Gly Arg Asp Ala Leu Val Gln 
        435                 440                 445             


Ala Val Arg Ala Gly Arg Ala Arg Val Glu Ala Trp Arg Ala Ala Arg 
    450                 455                 460                 


Arg 
465 


