                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  METALLOPROTEASE FROM KRIBBELLA ALUMINOSA

<130>  12917-WO-PCT

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  2043
<212>  DNA
<213>  Kribbella aluminosa


<220>
<221>  CDS
<222>  (1)..(2040)

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  The first amino acid should be Met, not Val. When the first codon
       is gtg a Met is inserted though gtg normally codes for Val.

<220>
<221>  sig_peptide
<222>  (1)..(60)

<220>
<221>  pro_peptide
<222>  (61)..(573)

<220>
<221>  mat_peptide
<222>  (574)..(2040)

<400>  1
gtg gcc  gtc gtc gcg gca gcg  ggt ctt gcg acg act  ttc acc gcc          45
Val Ala  Val Val Ala Ala Ala  Gly Leu Ala Thr Thr  Phe Thr Ala            
    -190                 -185                 -180                        

tcg acc  gcg ggc gcc gcc gac  cgg acg gct ccg ctc  cca ggc ttc          90
Ser Thr  Ala Gly Ala Ala Asp  Arg Thr Ala Pro Leu  Pro Gly Phe            
    -175                 -170                 -165                        

aac cag  ccg gcc gcc gtc cag  gcc gag cag gcg ctg  acc gcg cgg         135
Asn Gln  Pro Ala Ala Val Gln  Ala Glu Gln Ala Leu  Thr Ala Arg            
    -160                 -155                 -150                        

acg gcc  gcg gct ctc ggt ctc  ggc acc ggc gag cag  ctc aag gtc         180
Thr Ala  Ala Ala Leu Gly Leu  Gly Thr Gly Glu Gln  Leu Lys Val            
    -145                 -140                 -135                        

cgc gac  gtg gtg aag gac ccg  gac ggc acg gag tac  gtc cgc tac         225
Arg Asp  Val Val Lys Asp Pro  Asp Gly Thr Glu Tyr  Val Arg Tyr            
    -130                 -125                 -120                        

gac cgc  acc ttc aac ggc ctc  aag gtc gtc gga ggt  gac ctg atc         270
Asp Arg  Thr Phe Asn Gly Leu  Lys Val Val Gly Gly  Asp Leu Ile            
    -115                 -110                 -105                        

gtc aag  cgc aag ggc gag tcg atc ggc cag gtc acg tac aac cgc ggc       318
Val Lys  Arg Lys Gly Glu Ser Ile Gly Gln Val Thr Tyr Asn Arg Gly          
    -100                 -95                 -90                          

gcc aag gcc gtc gcc gtc gcc acc aag ccg acg ctg tcc cag tcg gcg        366
Ala Lys Ala Val Ala Val Ala Thr Lys Pro Thr Leu Ser Gln Ser Ala           
-85                 -80                 -75                 -70           

gcg ctc gcg aag ggt gcg caa gcc gcg gag ttc aag gcc acc ggc aac        414
Ala Leu Ala Lys Gly Ala Gln Ala Ala Glu Phe Lys Ala Thr Gly Asn           
                -65                 -60                 -55               

aag ggc gag ttg gtc gtc ttc gtg acg ccg acc aag cct gtc ctg gcg        462
Lys Gly Glu Leu Val Val Phe Val Thr Pro Thr Lys Pro Val Leu Ala           
            -50                 -45                 -40                   

tac gag gtc gtg acc acc ggt gtg aag gct gac cag acc cct tcg gtg        510
Tyr Glu Val Val Thr Thr Gly Val Lys Ala Asp Gln Thr Pro Ser Val           
        -35                 -30                 -25                       

ctg cac tcg ttc atc gac gcc aag acc ggc gcc gtg ctc gac cag gac        558
Leu His Ser Phe Ile Asp Ala Lys Thr Gly Ala Val Leu Asp Gln Asp           
    -20                 -15                 -10                           

gac gag gtc aag acc ggt acc ggc aac tcg atg tac tcc gga acc gtc        606
Asp Glu Val Lys Thr Gly Thr Gly Asn Ser Met Tyr Ser Gly Thr Val           
-5              -1  1               5                   10                

agc atc ggt acg tcg ggg agc tac acg atg agc gac ccg acc cgc ggc        654
Ser Ile Gly Thr Ser Gly Ser Tyr Thr Met Ser Asp Pro Thr Arg Gly           
            15                  20                  25                    

ggc aac tac acc acg gac ctc aac ggc tcc acc tcc ggc agc ggt acg        702
Gly Asn Tyr Thr Thr Asp Leu Asn Gly Ser Thr Ser Gly Ser Gly Thr           
        30                  35                  40                        

acg ttc acc gac cct gac gac acc tgg ggc aac ggc tcg acg tcc agc        750
Thr Phe Thr Asp Pro Asp Asp Thr Trp Gly Asn Gly Ser Thr Ser Ser           
    45                  50                  55                            

cgc cag acc gcc ggt gtg gac gcg cat tac ggc gcc cag ctg acc tgg        798
Arg Gln Thr Ala Gly Val Asp Ala His Tyr Gly Ala Gln Leu Thr Trp           
60                  65                  70                  75            

gac tac tac aag aac gtc cac ggc cgg aac ggc atc ttc aac aac ggc        846
Asp Tyr Tyr Lys Asn Val His Gly Arg Asn Gly Ile Phe Asn Asn Gly           
                80                  85                  90                

cag ggc gcg cgg tcc cgc gtg cac tac ggc aac gcg tac gtc aac gcg        894
Gln Gly Ala Arg Ser Arg Val His Tyr Gly Asn Ala Tyr Val Asn Ala           
            95                  100                 105                   

ttc tgg gac ggc acc cag atg acg tac ggc gac ggc gcg agc aac gcc        942
Phe Trp Asp Gly Thr Gln Met Thr Tyr Gly Asp Gly Ala Ser Asn Ala           
        110                 115                 120                       

cgt ccg ctg acc tcg atc gac gtc gcc ggc cac gag atg agc cac ggc        990
Arg Pro Leu Thr Ser Ile Asp Val Ala Gly His Glu Met Ser His Gly           
    125                 130                 135                           

gtc acc gag gcg acc gcg aac ctc aac tac tcc ggt gat gcg ggc ggc       1038
Val Thr Glu Ala Thr Ala Asn Leu Asn Tyr Ser Gly Asp Ala Gly Gly           
140                 145                 150                 155           

ctg aac gag gcg acc tcg gac atc ttc ggt acg gcg gtg gag ttc tcc       1086
Leu Asn Glu Ala Thr Ser Asp Ile Phe Gly Thr Ala Val Glu Phe Ser           
                160                 165                 170               

gcg aac aac tcg tcc gac ccg ggt gac tac ctg atc ggc gag aag atc       1134
Ala Asn Asn Ser Ser Asp Pro Gly Asp Tyr Leu Ile Gly Glu Lys Ile           
            175                 180                 185                   

aac atc aac ggc aac ggc acg ccg ctg cgc tac atg gac aag ccg tcg       1182
Asn Ile Asn Gly Asn Gly Thr Pro Leu Arg Tyr Met Asp Lys Pro Ser           
        190                 195                 200                       

aag gac ggc cgc agc gtg gac tgc tgg tcg acc agc acc ggc ggc ctg       1230
Lys Asp Gly Arg Ser Val Asp Cys Trp Ser Thr Ser Thr Gly Gly Leu           
    205                 210                 215                           

gac ccg cac tac tcg tcc ggc ccg ctg aac cac tgg ttc tac ctg gcc       1278
Asp Pro His Tyr Ser Ser Gly Pro Leu Asn His Trp Phe Tyr Leu Ala           
220                 225                 230                 235           

tcc gag ggc acc ggc agc aag gtc atc ggc ggc gtc acg cac agc agc       1326
Ser Glu Gly Thr Gly Ser Lys Val Ile Gly Gly Val Thr His Ser Ser           
                240                 245                 250               

acc gcc tgc aac ggc gcg acc atc acc ggt gtc ggc cgc gac gtg gcc       1374
Thr Ala Cys Asn Gly Ala Thr Ile Thr Gly Val Gly Arg Asp Val Ala           
            255                 260                 265                   

gcc aag gtc tgg tac cgc acg ctc agc acc aag ctg agc tcg ggc agc       1422
Ala Lys Val Trp Tyr Arg Thr Leu Ser Thr Lys Leu Ser Ser Gly Ser           
        270                 275                 280                       

acc tac aag gac gcc cgc gag ggt gcg atc aac tcc gcg aag gag ctg       1470
Thr Tyr Lys Asp Ala Arg Glu Gly Ala Ile Asn Ser Ala Lys Glu Leu           
    285                 290                 295                           

tac ggc gcg gac tcg gcg cag tgc aag ggc atc gag gcc gcg ttc aac       1518
Tyr Gly Ala Asp Ser Ala Gln Cys Lys Gly Ile Glu Ala Ala Phe Asn           
300                 305                 310                 315           

ggc atc tcg gtc ccg gct ggt gcg gcc gcc tgt ggt ggc ggg acc gac       1566
Gly Ile Ser Val Pro Ala Gly Ala Ala Ala Cys Gly Gly Gly Thr Asp           
                320                 325                 330               

ccg gag ccg ccg acg ggt ggc aac ctg ctg aag aac ccg ggt ttc gag       1614
Pro Glu Pro Pro Thr Gly Gly Asn Leu Leu Lys Asn Pro Gly Phe Glu           
            335                 340                 345                   

tcc ggc gcg gtg gac tgg acc ggc acc gcc ggc ccg atc acc aac gac       1662
Ser Gly Ala Val Asp Trp Thr Gly Thr Ala Gly Pro Ile Thr Asn Asp           
        350                 355                 360                       

tcg ggc cgc ccg gcc cgg acc ggc act tgg aag ctc tgg ctc gga ggg       1710
Ser Gly Arg Pro Ala Arg Thr Gly Thr Trp Lys Leu Trp Leu Gly Gly           
    365                 370                 375                           

aac ggc cgc acc gtc acc gag aac gtc gga cag tcg gtc gcg atc ccg       1758
Asn Gly Arg Thr Val Thr Glu Asn Val Gly Gln Ser Val Ala Ile Pro           
380                 385                 390                 395           

gcg tcg gcg acc agc gcg acc ctg tcg ttc tgg atc cgg atc gac acc       1806
Ala Ser Ala Thr Ser Ala Thr Leu Ser Phe Trp Ile Arg Ile Asp Thr           
                400                 405                 410               

gcg gag acc acc tct tcg acg gtg tac gac cgg gcg ttg gtg cag atc       1854
Ala Glu Thr Thr Ser Ser Thr Val Tyr Asp Arg Ala Leu Val Gln Ile           
            415                 420                 425                   

gtc gac ggg tcg acc acg acc acg ctg gcg acg tac tcg aac ctg aac       1902
Val Asp Gly Ser Thr Thr Thr Thr Leu Ala Thr Tyr Ser Asn Leu Asn           
        430                 435                 440                       

aag aac acg tcg tac atc cag aag acg ctg aac gtg acg gcg tac aag       1950
Lys Asn Thr Ser Tyr Ile Gln Lys Thr Leu Asn Val Thr Ala Tyr Lys           
    445                 450                 455                           

gga aag acc gtg acg gtg aag ttc gtc ggc cag gag gac tcc tcg ctg       1998
Gly Lys Thr Val Thr Val Lys Phe Val Gly Gln Glu Asp Ser Ser Leu           
460                 465                 470                 475           

cag acc agc ttc gtc atc gac gac acc tcc ctg acc gcg agc tga           2043
Gln Thr Ser Phe Val Ile Asp Asp Thr Ser Leu Thr Ala Ser                   
                480                 485                                   


<210>  2
<211>  680
<212>  PRT
<213>  Kribbella aluminosa

<400>  2

Val Ala  Val Val Ala Ala Ala  Gly Leu Ala Thr Thr  Phe Thr Ala 
    -190                 -185                 -180             


Ser Thr  Ala Gly Ala Ala Asp  Arg Thr Ala Pro Leu  Pro Gly Phe 
    -175                 -170                 -165             


Asn Gln  Pro Ala Ala Val Gln  Ala Glu Gln Ala Leu  Thr Ala Arg 
    -160                 -155                 -150             


Thr Ala  Ala Ala Leu Gly Leu  Gly Thr Gly Glu Gln  Leu Lys Val 
    -145                 -140                 -135             


Arg Asp  Val Val Lys Asp Pro  Asp Gly Thr Glu Tyr  Val Arg Tyr 
    -130                 -125                 -120             


Asp Arg  Thr Phe Asn Gly Leu  Lys Val Val Gly Gly  Asp Leu Ile 
    -115                 -110                 -105             


Val Lys  Arg Lys Gly Glu Ser Ile Gly Gln Val Thr Tyr Asn Arg Gly 
    -100                 -95                 -90                 


Ala Lys Ala Val Ala Val Ala Thr Lys Pro Thr Leu Ser Gln Ser Ala 
-85                 -80                 -75                 -70 


Ala Leu Ala Lys Gly Ala Gln Ala Ala Glu Phe Lys Ala Thr Gly Asn 
                -65                 -60                 -55     


Lys Gly Glu Leu Val Val Phe Val Thr Pro Thr Lys Pro Val Leu Ala 
            -50                 -45                 -40         


Tyr Glu Val Val Thr Thr Gly Val Lys Ala Asp Gln Thr Pro Ser Val 
        -35                 -30                 -25             


Leu His Ser Phe Ile Asp Ala Lys Thr Gly Ala Val Leu Asp Gln Asp 
    -20                 -15                 -10                 


Asp Glu Val Lys Thr Gly Thr Gly Asn Ser Met Tyr Ser Gly Thr Val 
-5              -1  1               5                   10      


Ser Ile Gly Thr Ser Gly Ser Tyr Thr Met Ser Asp Pro Thr Arg Gly 
            15                  20                  25          


Gly Asn Tyr Thr Thr Asp Leu Asn Gly Ser Thr Ser Gly Ser Gly Thr 
        30                  35                  40              


Thr Phe Thr Asp Pro Asp Asp Thr Trp Gly Asn Gly Ser Thr Ser Ser 
    45                  50                  55                  


Arg Gln Thr Ala Gly Val Asp Ala His Tyr Gly Ala Gln Leu Thr Trp 
60                  65                  70                  75  


Asp Tyr Tyr Lys Asn Val His Gly Arg Asn Gly Ile Phe Asn Asn Gly 
                80                  85                  90      


Gln Gly Ala Arg Ser Arg Val His Tyr Gly Asn Ala Tyr Val Asn Ala 
            95                  100                 105         


Phe Trp Asp Gly Thr Gln Met Thr Tyr Gly Asp Gly Ala Ser Asn Ala 
        110                 115                 120             


Arg Pro Leu Thr Ser Ile Asp Val Ala Gly His Glu Met Ser His Gly 
    125                 130                 135                 


Val Thr Glu Ala Thr Ala Asn Leu Asn Tyr Ser Gly Asp Ala Gly Gly 
140                 145                 150                 155 


Leu Asn Glu Ala Thr Ser Asp Ile Phe Gly Thr Ala Val Glu Phe Ser 
                160                 165                 170     


Ala Asn Asn Ser Ser Asp Pro Gly Asp Tyr Leu Ile Gly Glu Lys Ile 
            175                 180                 185         


Asn Ile Asn Gly Asn Gly Thr Pro Leu Arg Tyr Met Asp Lys Pro Ser 
        190                 195                 200             


Lys Asp Gly Arg Ser Val Asp Cys Trp Ser Thr Ser Thr Gly Gly Leu 
    205                 210                 215                 


Asp Pro His Tyr Ser Ser Gly Pro Leu Asn His Trp Phe Tyr Leu Ala 
220                 225                 230                 235 


Ser Glu Gly Thr Gly Ser Lys Val Ile Gly Gly Val Thr His Ser Ser 
                240                 245                 250     


Thr Ala Cys Asn Gly Ala Thr Ile Thr Gly Val Gly Arg Asp Val Ala 
            255                 260                 265         


Ala Lys Val Trp Tyr Arg Thr Leu Ser Thr Lys Leu Ser Ser Gly Ser 
        270                 275                 280             


Thr Tyr Lys Asp Ala Arg Glu Gly Ala Ile Asn Ser Ala Lys Glu Leu 
    285                 290                 295                 


Tyr Gly Ala Asp Ser Ala Gln Cys Lys Gly Ile Glu Ala Ala Phe Asn 
300                 305                 310                 315 


Gly Ile Ser Val Pro Ala Gly Ala Ala Ala Cys Gly Gly Gly Thr Asp 
                320                 325                 330     


Pro Glu Pro Pro Thr Gly Gly Asn Leu Leu Lys Asn Pro Gly Phe Glu 
            335                 340                 345         


Ser Gly Ala Val Asp Trp Thr Gly Thr Ala Gly Pro Ile Thr Asn Asp 
        350                 355                 360             


Ser Gly Arg Pro Ala Arg Thr Gly Thr Trp Lys Leu Trp Leu Gly Gly 
    365                 370                 375                 


Asn Gly Arg Thr Val Thr Glu Asn Val Gly Gln Ser Val Ala Ile Pro 
380                 385                 390                 395 


Ala Ser Ala Thr Ser Ala Thr Leu Ser Phe Trp Ile Arg Ile Asp Thr 
                400                 405                 410     


Ala Glu Thr Thr Ser Ser Thr Val Tyr Asp Arg Ala Leu Val Gln Ile 
            415                 420                 425         


Val Asp Gly Ser Thr Thr Thr Thr Leu Ala Thr Tyr Ser Asn Leu Asn 
        430                 435                 440             


Lys Asn Thr Ser Tyr Ile Gln Lys Thr Leu Asn Val Thr Ala Tyr Lys 
    445                 450                 455                 


Gly Lys Thr Val Thr Val Lys Phe Val Gly Gln Glu Asp Ser Ser Leu 
460                 465                 470                 475 


Gln Thr Ser Phe Val Ile Asp Asp Thr Ser Leu Thr Ala Ser 
                480                 485                 


<210>  3
<211>  27
<212>  PRT
<213>  Bacillus clausii

<400>  3

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala 
            20                  25          


<210>  4
<211>  300
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  4

Ala Ala Thr Thr Gly Thr Gly Thr Thr Leu Lys Gly Lys Thr Val Ser 
1               5                   10                  15      


Leu Asn Ile Ser Ser Glu Ser Gly Lys Tyr Val Leu Arg Asp Leu Ser 
            20                  25                  30          


Lys Pro Thr Gly Thr Gln Ile Ile Thr Tyr Asp Leu Gln Asn Arg Glu 
        35                  40                  45              


Tyr Asn Leu Pro Gly Thr Leu Val Ser Ser Thr Thr Asn Gln Phe Thr 
    50                  55                  60                  


Thr Ser Ser Gln Arg Ala Ala Val Asp Ala His Tyr Asn Leu Gly Lys 
65                  70                  75                  80  


Val Tyr Asp Tyr Phe Tyr Gln Lys Phe Asn Arg Asn Ser Tyr Asp Asn 
                85                  90                  95      


Lys Gly Gly Lys Ile Val Ser Ser Val His Tyr Gly Ser Arg Tyr Asn 
            100                 105                 110         


Asn Ala Ala Trp Ile Gly Asp Gln Met Ile Tyr Gly Asp Gly Asp Gly 
        115                 120                 125             


Ser Phe Phe Ser Pro Leu Ser Gly Ser Met Asp Val Thr Ala His Glu 
    130                 135                 140                 


Met Thr His Gly Val Thr Gln Glu Thr Ala Asn Leu Asn Tyr Glu Asn 
145                 150                 155                 160 


Gln Pro Gly Ala Leu Asn Glu Ser Phe Ser Asp Val Phe Gly Tyr Phe 
                165                 170                 175     


Asn Asp Thr Glu Asp Trp Asp Ile Gly Glu Asp Ile Thr Val Ser Gln 
            180                 185                 190         


Pro Ala Leu Arg Ser Leu Ser Asn Pro Thr Lys Tyr Gly Gln Pro Asp 
        195                 200                 205             


Asn Phe Lys Asn Tyr Lys Asn Leu Pro Asn Thr Asp Ala Gly Asp Tyr 
    210                 215                 220                 


Gly Gly Val His Thr Asn Ser Gly Ile Pro Asn Lys Ala Ala Tyr Asn 
225                 230                 235                 240 


Thr Ile Thr Lys Ile Gly Val Asn Lys Ala Glu Gln Ile Tyr Tyr Arg 
                245                 250                 255     


Ala Leu Thr Val Tyr Leu Thr Pro Ser Ser Thr Phe Lys Asp Ala Lys 
            260                 265                 270         


Ala Ala Leu Ile Gln Ser Ala Arg Asp Leu Tyr Gly Ser Gln Asp Ala 
        275                 280                 285             


Ala Ser Val Glu Ala Ala Trp Asn Ala Val Gly Leu 
    290                 295                 300 


<210>  5
<211>  269
<212>  PRT
<213>  Bacillus clausii

<400>  5

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


