               SEQUENCE LISTING

<110> Academia Sinica

<120> HUMANIZED ANTI-IgE ANTIBODIES THAT CROSSLINK CD23 ON B LYMPHOCYTES BUT DO NOT SENSITIZE MAST CELLS

<130> P2800-PCT

<150> US62013196
<151> 2014-06-17

<160> 12

<170> BiSSAP 1.3

<210> 1
<211> 119
<212> PRT
<213> Mus sp.

<220> 
<223> variable region of heavy chain derived from 8D6 hybridoma

<400> 1
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      
Ser Val Met Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Asn Gly Tyr 
            20                  25                  30          
Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Asp Leu Glu Trp Ile 
        35                  40                  45              
Gly Tyr Ile Asn Pro Thr Thr Gly His Thr Glu Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Asp Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  
Ile Glu Leu Ser Ser Leu Thr Ser Asp Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gln Glu Tyr Arg His Ser Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ala 
        115                 

<210> 2
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> humanized variable region of heavy chain

<400> 2
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Asn Gly Tyr 
            20                  25                  30          
Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Tyr Ile Asn Pro Thr Thr Gly His Thr Glu Tyr Asn Gln Lys Phe 
    50                  55                  60                  
Lys Asp Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gln Glu Tyr Arg His Ser Trp Phe Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 3
<211> 111
<212> PRT
<213> Mus sp.

<220> 
<223> variable region of light chain derived from 8D6 hybridoma

<400> 3
Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      
Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          
Gly Asp Thr Tyr Met Asn Trp Tyr His Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              
Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Asp Ser Gly Ile Pro Ala 
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  
Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Thr Asn 
                85                  90                  95      
Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     

<210> 4
<211> 111
<212> PRT
<213> Artificial Sequence

<220> 
<223> humanized variable region of light chain

<400> 4
Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Val Asp Tyr Asp 
            20                  25                  30          
Gly Asp Thr Tyr Met Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              
Lys Leu Leu Ile Tyr Ala Ala Ser Asn Leu Asp Ser Gly Val Pro Ser 
    50                  55                  60                  
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  
Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Thr Asn 
                85                  90                  95      
Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110     

<210> 5
<211> 10
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 1 derived from the heavy chain
      of mu8D6 antibody

<400> 5
Gly Tyr Thr Phe Asn Gly Tyr Trp Met His 
1               5                   10  

<210> 6
<211> 17
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 2 derived from the heavy chain
      of mu8D6 antibody

<400> 6
Tyr Ile Asn Pro Thr Thr Gly His Thr Glu Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      
Asp 
    

<210> 7
<211> 12
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 3 derived from the heavy chain
      of mu8D6 antibody

<400> 7
Ala Arg Gln Glu Tyr Arg His Ser Trp Phe Ala Tyr 
1               5                   10          

<210> 8
<211> 10
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 1 derived from the light chain
      of mu8D6 antibody

<400> 8
Gln Ser Val Asp Tyr Asp Gly Asp Thr Tyr 
1               5                   10  

<210> 9
<211> 7
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 2 derived from the light chain
      of mu8D6 antibody

<400> 9
Ala Ala Ser Asn Leu Asp Ser 
1               5           

<210> 10
<211> 9
<212> PRT
<213> Mus sp.

<220> 
<223> complementarity determining region 3 derived from the light chain
      of mu8D6 antibody

<400> 10
Gln Gln Thr Asn Glu Asp Pro Trp Thr 
1               5                   

<210> 11
<211> 113
<212> PRT
<213> Homo sapiens

<220> 
<223> the germline VH1-69/JH4 sequence of human immunoglobulin gene

<400> 11
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Gly Thr Phe Ser Ser Tyr 
            20                  25                  30          
Ala Ile Ser Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Gly Ile Ile Pro Ile Phe Gly Thr Ala Asn Tyr Ala Gln Lys Phe 
    50                  55                  60                  
Gln Gly Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         
Ser 
    

<210> 12
<211> 107
<212> PRT
<213> Homo sapiens

<220> 
<223> the germline Vk1-39/Jk1 sequence of human immunoglobulin gene

<400> 12
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          
Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ala Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Trp Thr 
                85                  90                  95      
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105         

