
                                SEQUENCE LISTING

<110> Saville, Renee M.
      Silverman, Joshua
      Minshull, Jeremy
      Ness, Jon
      Leonard, Effendi
      Stumpe, Jana

<120> NUCLEIC ACIDS AND VECTORS FOR USE WITH
  METHANOTROPHIC BACTERIA
  

<130> 200206.413WO

<140> US                  
<141> 2015-06-18  

<150> US 62/014,007       
<151> 2014-06-18  

<160> 54

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 710
<212> DNA
<213> Artificial Sequence

<220> 
<223> minimal oriV (modified)

<400> 1
gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg      60
aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg     120
cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa     180
agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct     240
ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc     300
actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa     360
gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc     420
gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa     480
tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac     540
gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg     600
gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac     660
ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcggaaagg                710

<210> 2
<211> 1081
<212> DNA
<213> Artificial Sequence

<220> 
<223> oriV (modified)

<400> 2
tgacacttga ggggcgttta gagcgagcca ggaaagccga ccccctcctt ggagtaaaaa      60
cccttgcggc gttgcagccg gcacggatct tccgatcggg cgcggtggtg gccgcgtctg     120
tgacctaaaa aggggggagt ccagaggggc gcagcccctt tgggcatagc gcagcgtaat     180
cggagacgta attgagcatt tccaggcgct tgcgcctggt caacgaaaga gtcagcgccg     240
taggcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg     300
atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc     360
gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt     420
ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa     480
atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg     540
tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc     600
ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa     660
ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc     720
cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg     780
tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc     840
cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca     900
tagacggccg ccagcccagc ggcgagggca accagcccgg tgagcgtcgg aaaggcgctg     960
gaagccccgt agcgacgcgg agaggggcga gacaagccaa gggcgcaggc tcgatgcgca    1020
gcacgacata gccggttctc gcaaggacga gaatttccct gcggtgcccc tcaagtgtca    1080
a                                                                    1081

<210> 3
<211> 1146
<212> DNA
<213> Pseudomonas aeruginosa plasmid RK2

<400> 3
atgaatcgga cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc      60
gccgaggatg ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc     120
cagtccgtcg gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa     180
ctggctcccc ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtctagaa     240
caggaggcgg caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc     300
aagaagcgaa aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc     360
gcgttgctga aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt     420
gcgccgtggc cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc     480
accacgcgca acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc     540
aacaaggacg tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg     600
gtgtggcagc aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc     660
acgttctacg agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag     720
gccgaggaat gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt     780
gggcacctgg aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa     840
acgtcccgtt gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac     900
tacacgaaat tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc     960
gactatttca gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg    1020
tgcggatcgg attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa    1080
gagttgcgag gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc    1140
aaacgc                                                               1146

<210> 4
<211> 178
<212> DNA
<213> Artificial Sequence

<220> 
<223> trfA promoter 1 (immediately upstream trfA in RK2)

<400> 4
attcgtgcag ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg      60
gaccgacttc attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa     120
tcaggaataa gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtga       178

<210> 5
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> trfA promoter 2 (the promoter from the trf operon
      in RK2)

<400> 5
ttgacagcgg aaccaatgtt tagctaaact agagtctcct ttctcaagga gactttcgat      60

<210> 6
<211> 201
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pbla promoter

<400> 6
agacgaaagg gcctcgtgat acgcttattt ttataggtta atgtcatgat aataatggtt      60
tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt     120
ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     180
taatattgaa aaaggaagag t                                               201

<210> 7
<211> 170
<212> DNA
<213> Artificial Sequence

<220> 
<223> Pneo promoter

<400> 7
acagcaagcg aaccggaatt gccagctggc gcgccctctg gtaaggttgg gaagccctgc      60
aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatcg     120
acggatcgat ccggggaatt aattccgggg caatcccgca aggagggtga                170

<210> 8
<211> 742
<212> DNA
<213> Artificial Sequence

<220> 
<223> minimal RK2 origin of transfer (oriT)

<400> 8
cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc      60
ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat     120
ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt     180
cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg     240
gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac     300
caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa gagcgattga     360
ggaaaaggcg gcggcggccg gcatgagcct gtaggcctac ctgctggccg tcggccaggg     420
ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc gcatcaatgg     480
cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc cgcgcacggc     540
gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga agcaggacga     600
gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac ttttttagcc     660
gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc tccatcaaga     720
agagcgactt cgcggagctg gt                                              742

<210> 9
<211> 671
<212> DNA
<213> Artificial Sequence

<220> 
<223> kanamycin resistance cassette promoter

<400> 9
ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact ggcatttcag      60
gaacaagcgg gcactgctcg acgcacttgc ttcgctcagt atcgctcggg acgcacggcg     120
cgctctacga actgccgata aacagaggat taaaattgac aattctaggg cgcgtatagc     180
ttgccggaag tcgccttgac ccgcatggca taggcctatc gtttccacga tcagcgatcg     240
gctcgttgcc ctgcgccgct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag     300
agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc     360
gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct     420
tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc     480
attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc     540
ggacctgcag gggggggggg gaaagccacg ttgtgtctca aaatctctga tgttacattg     600
cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata     660
caaggggtgt t                                                          671

<210> 10
<211> 807
<212> DNA
<213> Artificial Sequence

<220> 
<223> kanamycin resistance cassette coding sequence

<400> 10
atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat      60
ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc     120
ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc     180
aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gccacttccg     240
accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc     300
ggaaaaacag cgttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat     360
gcgctggcag tgttcctgcg ccggttgcac tcgattcctg tttgtaattg tccttttaac     420
agcgatcgcg tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat     480
gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg     540
cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat     600
aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc     660
gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca     720
ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag     780
tttcatttga tgctcgatga gtttttc                                         807

<210> 11
<211> 422
<212> DNA
<213> Artificial Sequence

<220> 
<223> promoter of Kanamycin resistance cassette from RK2

<400> 11
ttgacaacgt gcctggatct ggctacacta tgcggccacc aagctcgccc gtggcgagct      60
ttacgaagcg atcggcatgc tcggtttctt ccgtgagcaa gtgttaggac ctttgctcta     120
ccgtcgcgct ggaaaggacc agcgcggagt gaggcgattg gaaacccttc gactggatga     180
agagcgcaga ctagccacca ccattgcgct gcacgatgcg ttgtctgtca gggatgccat     240
caaagcatct gcctccatct atctcgacct ccgagccgcc gatccgtcgt tggaaccgac     300
aacgcatatg ccaggtcttc tgtacgactt aatagaacgt gcggtaccag gcacgcctaa     360
ccgtcagtga gattggatga gtgaacgata ttgatcgaga agagccctgc gcagccgctg     420
cc                                                                    422

<210> 12
<211> 822
<212> DNA
<213> Pseudomonas aeruginosa plasmid RK2

<400> 12
gtgcccgaga gcatggcggc tcacgtgatg ggatacaaat gggcgcgtga taaggttggt      60
cagtccggct gcgcggtcta tcggctgcat agcaagtcag gcggctccga cttgtttctg     120
aagcacggca aagatgcttt tgccgacgac gtgactgatg aaatggtgag attgcgttgg     180
ctggcggggc acatttctgt gccctccgtt gtaagcttcg ttcgcacgcc caatcaggca     240
tggctcctga caacagcaat acatggaaaa acggcatatc aagtgctgaa atcggatttc     300
ggagcccgtc tcgttgttgt tgacgcattg gcggcgttca tgcgccgact gcatgcgatc     360
ccagtgagcg aatgctcctt caacagtgac cacgcatgca ggcttgcccg agcgcgggag     420
cgtatcgagg cgggggttgt tgatgtcgat gacttcgata aggagcgcga agggtggacg     480
gccgaacagg tttgggaggc gatgcatcgc ctcctaccgc tcgcgccgga cccagtcgtg     540
acgcacggcg atttttcact cgataatcta cttatcgtcg aaggtaaggt agtcggctgc     600
atcgacgttg ggcgggctgg tattgctgat cgataccaag accttgccgt gttatggaac     660
tgtcttgagg agttcgaacc ttcgcttcag gagaggcttg ttgcgcaata tggcattgcc     720
gatccggata ggcgcaagct gcaatttcat ctcctgctgg acgaactttt ctaaggcgat     780
gccccctcga cctcgatcag ggaggcgttc aggacgactc ac                        822

<210> 13
<211> 1014
<212> DNA
<213> Artificial Sequence

<220> 
<223> pUC origin of replication

<400> 13
tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc      60
gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc     120
ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg     180
tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag     240
cgcagatacc aaatactgtt cttctagtgt agccgtagtt agcccaccac ttcaagaact     300
ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg     360
gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc     420
ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg     480
aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg     540
cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag     600
ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc     660
gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct     720
ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc     780
ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc     840
gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga aggcgagagt agggaactgc     900
caggcatcaa actaagcaga aggcccctga cggatggcct ttttgcgttt ctacaaactc     960
tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc cccctgggcg gttc          1014

<210> 14
<211> 229
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative methanol dehydrogenase (MDH), large
      subunit (MCA0779) promoter with native RBS and
      spacer

<400> 14
tttgcctcga tcggcggtcc ttgtgacagg gagatattcc cgacggatcc ggggcattcg      60
agcggaaccg cccgccgtgg gagtttttcc agcgagcatt cgagagtttt tcaaggcggc     120
ttcgaggggt tattccgtaa cgccgccgac atgatctgtc ccagaatctc cgccgctgtt     180
cgtagagcgc cgatgcaggg tcggcatcaa tcattcttgg aggagacac                 229

<210> 15
<211> 150
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative 30S ribosomal protein S16 (MCA0392)
      promoter with native RBS and spacer

<400> 15
gttcaggata agcccggtgc cggatggcga ccgggttttt cttttcaacc ctggtgcttc      60
ccagtggggc aagacggcag ggcttcacaa ttactcagga tcgcatacaa tacgcgtttt     120
tatccgtacg gtttgactag aggcacaggc                                      150

<210> 16
<211> 497
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative hexulose 6-phosphate synthase promoter
      from Methylomonas 16a with native RBS and spacer

<400> 16
ttcggaatcc ctgacgggaa ttggcccgaa gaaggcagat gccatcgttc agtatcgaaa      60
ggaacatggg gattttcagt cattgaagga tctggagaat gtcagcggca ttggcgagaa     120
aacccttcag gccaatgaaa aagacattcg cttcacggat gatttgagcg ataagtcatc     180
cgcggaaaaa ggtgcggtag ctgtggataa aaaaggcgcc agatagtaag cgctaaggat     240
tggggtgcgt cgccggtcgc ggcggcgctc ctcgacggca gagttggtgc caggttggcg     300
gatgattgat gccgaatatt acgcgaccaa ttctcgaggc aaatgaactg tgagctactg     360
agttgcaggc attgacagcc atcccatttc tatcatacag ttacggacgc atcacgagta     420
ggtgataagc ctagcagatt gcggcagttg gcaaaatcag ctattactaa taattaaaaa     480
ctttcggagc acatcac                                                    497

<210> 17
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> T5 promoter

<400> 17
aaatcatgaa aaatttattt gctttgtgag cggataacaa ttataata                   48

<210> 18
<211> 89
<212> DNA
<213> Artificial Sequence

<220> 
<223> trc promoter

<400> 18
attctgaaat gagctgttga caattaatca tccggctcgt ataacggata acaattcccc      60
tctagaaata attttgttta actttaaga                                        89

<210> 19
<211> 256
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative MDH, large subunit (MCA0779) promoter
      linked to a variant MDH ribosome binding site
      (RBS_1.0) with variant and modified spacer

<400> 19
ggtctccttt ttcgtcttcg aattctttgc ctcgatcggc ggtccttgtg acagggagat      60
attcccgacg gatccggggc attcgagcgg aaccgcccgc cgtgggagtt tttccagcga     120
gcattcgaga gtttttcaag gcggcttcga ggggttattc cgtaacgccg ccgacatgat     180
ctgtcccaga atctccgccg ctgttcgtag agcgccgatg cagggtcggc atcaatcatt     240
ctaaggaggt aaaaaa                                                     256

<210> 20
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant MDH, large subunit (MCA0779) RBS (RBS_1.0)
      with variant and modified spacer

<400> 20
aaggaggtaa aaaa                                                        14

<210> 21
<211> 256
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative MDH, large subunit (MCA0779) promoter
      linked to a variant MDH ribosome binding site
      (RBS_0.6) with variant and modified spacer

<400> 21
ggtctccttt ttcgtcttcg aattctttgc ctcgatcggc ggtccttgtg acagggagat      60
attcccgacg gatccggggc attcgagcgg aaccgcccgc cgtgggagtt tttccagcga     120
gcattcgaga gtttttcaag gcggcttcga ggggttattc cgtaacgccg ccgacatgat     180
ctgtcccaga atctccgccg ctgttcgtag agcgccgatg cagggtcggc atcaatcatt     240
ctatggtgga aaaaaa                                                     256

<210> 22
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant MDH, large subunit (MCA0779) RBS (RBS_0.6)
      with variant and modified spacer

<400> 22
atggtggaaa aaaa                                                        14

<210> 23
<211> 263
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative MDH, large subunit (MCA0779) promoter
      linked to variant MDH ribosome binding site
      (RBS_0.15) with variant and modified spacer

<400> 23
ggtctccttt ttcgtcttcg aattctttgc ctcgatcggc ggtccttgtg acagggagat      60
attcccgacg gatccggggc attcgagcgg aaccgcccgc cgtgggagtt tttccagcga     120
gcattcgaga gtttttcaag gcggcttcga ggggttattc cgtaacgccg ccgacatgat     180
ctgtcccaga atctccgccg ctgttcgtag agcgccgatg cagggtcggc atcaatcatt     240
ctatggatgt aaaaaataaa aaa                                             263

<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> modified MDH, large subunit (MCA0779) RBS
      (RBS_0.15) with variant and modified spacer

<400> 24
atggatgtaa aaaataaaaa a                                                21

<210> 25
<211> 263
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative MDH, large subunit (MCA0779) promoter
      linked to variant MDH ribosome binding site
      (RBS_0.1) with variant and modified spacer

<400> 25
ggtctccttt ttcgtcttcg aattctttgc ctcgatcggc ggtccttgtg acagggagat      60
attcccgacg gatccggggc attcgagcgg aaccgcccgc cgtgggagtt tttccagcga     120
gcattcgaga gtttttcaag gcggcttcga ggggttattc cgtaacgccg ccgacatgat     180
ctgtcccaga atctccgccg ctgttcgtag agcgccgatg cagggtcggc atcaatcatt     240
ctattgtggt aaaaaataaa aaa                                             263

<210> 26
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> modified MDH, large subunit (MCA0779) RBS
      (RBS_0.1) with variant and modified spacer

<400> 26
attgtggtaa aaaataaaaa a                                                21

<210> 27
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative native MDH, large subunit (MCA0779)
      ribosome binding site with modified spacer

<400> 27
tggaggagac a                                                           11

<210> 28
<211> 151
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative 30S ribosomal protein S16 (MCA0392)
      promoter linked to native MDH, large subunit
      (MCA0779) ribosome binding site with modified
      spacer

<400> 28
gttcaggata agcccggtgc cggatggcga ccgggttttt cttttcaacc ctggtgcttc      60
ccagtggggc aagacggcag ggcttcacaa ttactcagga tcgcatacaa tacgcgtttt     120
tatccgtacg gtttgactag tggaggagac a                                    151

<210> 29
<211> 496
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative hexulose 6-phosphate synthase promoter
      from Methylomonas 16a linked to native MDH, large
      subunit (MCA0779) ribosome binding site with
      modified spacer

<400> 29
ttcggaatcc ctgacgggaa ttggcccgaa gaaggcagat gccatcgttc agtatcgaaa      60
ggaacatggg gattttcagt cattgaagga tctggagaat gtcagcggca ttggcgagaa     120
aacccttcag gccaatgaaa aagacattcg cttcacggat gatttgagcg ataagtcatc     180
cgcggaaaaa ggtgcggtag ctgtggataa aaaaggcgcc agatagtaag cgctaaggat     240
tggggtgcgt cgccggtcgc ggcggcgctc ctcgacggca gagttggtgc caggttggcg     300
gatgattgat gccgaatatt acgcgaccaa ttctcgaggc aaatgaactg tgagctactg     360
agttgcaggc attgacagcc atcccatttc tatcatacag ttacggacgc atcacgagta     420
ggtgataagc ctagcagatt gcggcagttg gcaaaatcag ctattactaa taattaaaaa     480
ctttctggag gagaca                                                     496

<210> 30
<211> 695
<212> DNA
<213> Artificial Sequence

<220> 
<223> moxF (MDH, large subunit) promoter from
      Methylomonas 16a linked to native MDH, large
      subunit (MCA0779) ribosome binding site with
      modified spacer

<400> 30
gcgccgtatg ctttcgaatc cgccaagacc gggcatggat aaatccatga ccaccacatc      60
gggcaattgc tttgaataga gttggcaagc ggtctcgcca cgatcggctt catagatctc     120
gccgatacga tccgacagcg atagataggt cttgtagccg gtacgaacca cggcgtgatc     180
atctaccaat aaaacgctga ttttactcgc cactggaaaa tttcctcctc aggtcgtcaa     240
gggataaaga tatgggacaa gtccagtctg atgccaggcg gacttggtgt gccttttttt     300
atgatgacgc tttatccgtg cttaaaccat gggagctttt cccgtttcca atttcgatcc     360
ttggcgagat aggaatattt ccgtgcatga ttgcgtcgat ttcacatcga tttcatggat     420
tgttccgtaa cgttagccag cccggcttct ataacatttg cgccagcgtg gcctggtggt     480
cggtaacccg tgatgcggtt atgatcaaca aagctggttt tcaacgacta attctgatct     540
tcaggtcgcg cctcacttat agcgataaaa atcctggagg aaacatgcaa caactcgatt     600
tgcgcatagt cgggaaaacc gcggccttgt tggctggtgg ccttctgagc gtggcgcaac     660
ccgcatcggc gaacaaggag ctggtggagg agaca                                695

<210> 31
<211> 1235
<212> DNA
<213> Artificial Sequence

<220> 
<223> construct having  30S ribosomal protein S16
      promoter, modified MDH ribosomal binding 
      sequence, and variant lacI CDS
      

<400> 31
gttcaggata agcccggtgc cggatggcga ccgggttttt cttttcaacc ctggtgcttc      60
ccagtggggc aagacggcag ggcttcacaa ttactcagga tcgcatacaa tacgcgtttt     120
tatccgtacg gtttgactag tggaggagac atatgaaacc agtgactttg tacgatgtag     180
cggaatatgc aggcgtaagc taccagactg tgtcccgtgt cgttaaccaa gccagccacg     240
tgagcgcgaa aacgcgcgag aaggttgagg cagcaatggc ggaactgaat tacattccga     300
accgtgttgc tcaacagttg gccggcaagc agagcctgct gattggcgtt gcgacctcta     360
gcctggcgct gcacgcgagc agccaaatcg tggcagcgat taagtctcgt gcggatcaac     420
tgggtgcgag cgtcgtggtg agcatggtgg aacgtagcgg tgtcgaggct tgtaaagcgg     480
cagttcacaa tctgctggcc cagcgtgtca gcggcctgat tatcaattac ccgctggacg     540
accaggacgc aatcgctgtt gaggcggctt gcacgaatgt cccagcactg ttcttggacg     600
ttagcgatca gaccccgatt aactccatca tcttctccca tgaggacggc acccgcctgg     660
gcgtcgaaca tctggttgcg ctgggtcatc agcagatcgc gctgttggcg ggtccgttga     720
gcagcgtgag cgcgcgtctg cgtctggcgg gctggcacaa atatctgacg cgcaaccaaa     780
tccagcctat tgcggagcgc gaaggtgatt ggagcgcaat gtctggcttt cagcaaacga     840
tgcagatgct gaatgaaggt attgttccga ccgcgatgct ggttgttaac gatcagatgg     900
ccctgggtgc gatgcgtgca atcaccgaga gcggcctgcg cgttggtgcc gacatttccg     960
tcgtcggtta tgacgacacc gaggatagct cttgctacat cccaccgctg accaccatta    1020
aacaagattt tcgtctgctg ggtcaaacca gcgtcgatcg cctgctgcaa ttgtcgcagg    1080
gtcaggccgt gaaaggtaat caactgctgc cggtgagcct ggttaagcgt aagacgacgt    1140
tggcaccgaa cacccaaacc gcaagcccgc gtgctctggc cgacagcctg atgcaactgg    1200
cgcgtcaagt gtcgcgcctg gaaagcggtc agtaa                               1235

<210> 32
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> native MDH, large subunit (MCA0779) ribosome
      binding site with variant spacer (of SEQ ID NO:31)

<400> 32
tggaggagac at                                                          12

<210> 33
<211> 1083
<212> DNA
<213> Artificial Sequence

<220> 
<223> coding sequence of variant lacI (of SEQ ID NO:31)

<400> 33
atgaaaccag tgactttgta cgatgtagcg gaatatgcag gcgtaagcta ccagactgtg      60
tcccgtgtcg ttaaccaagc cagccacgtg agcgcgaaaa cgcgcgagaa ggttgaggca     120
gcaatggcgg aactgaatta cattccgaac cgtgttgctc aacagttggc cggcaagcag     180
agcctgctga ttggcgttgc gacctctagc ctggcgctgc acgcgagcag ccaaatcgtg     240
gcagcgatta agtctcgtgc ggatcaactg ggtgcgagcg tcgtggtgag catggtggaa     300
cgtagcggtg tcgaggcttg taaagcggca gttcacaatc tgctggccca gcgtgtcagc     360
ggcctgatta tcaattaccc gctggacgac caggacgcaa tcgctgttga ggcggcttgc     420
acgaatgtcc cagcactgtt cttggacgtt agcgatcaga ccccgattaa ctccatcatc     480
ttctcccatg aggacggcac ccgcctgggc gtcgaacatc tggttgcgct gggtcatcag     540
cagatcgcgc tgttggcggg tccgttgagc agcgtgagcg cgcgtctgcg tctggcgggc     600
tggcacaaat atctgacgcg caaccaaatc cagcctattg cggagcgcga aggtgattgg     660
agcgcaatgt ctggctttca gcaaacgatg cagatgctga atgaaggtat tgttccgacc     720
gcgatgctgg ttgttaacga tcagatggcc ctgggtgcga tgcgtgcaat caccgagagc     780
ggcctgcgcg ttggtgccga catttccgtc gtcggttatg acgacaccga ggatagctct     840
tgctacatcc caccgctgac caccattaaa caagattttc gtctgctggg tcaaaccagc     900
gtcgatcgcc tgctgcaatt gtcgcagggt caggccgtga aaggtaatca actgctgccg     960
gtgagcctgg ttaagcgtaa gacgacgttg gcaccgaaca cccaaaccgc aagcccgcgt    1020
gctctggccg acagcctgat gcaactggcg cgtcaagtgt cgcgcctgga aagcggtcag    1080
taa                                                                  1083

<210> 34
<211> 1031
<212> DNA
<213> Artificial Sequence

<220> 
<223> construct having first lacO (lacO1), putative MDH
      promoter, second lacO (lacO2), untranslated region, 
      altered MDH ribosomal binding sequence, and Dasher
      green fluorescent protein CDS
      
      
<400> 34
aattgtgagc ggataacaat ttttgcctcg atcggcggtc cttgtgacag ggagatattc      60
ccgacggatc cggggcattc gagcggaacc gcccgccgtg ggagtttttc cagcgagcat     120
tcgagagttt ttcaaggcgg cttcgagggg ttattccgta acgccgccga catgatctgt     180
cccagaatct ccgccgctgt tcgtagagcg ccgatgcagg gtcggcatca atcattcttg     240
gtgtggaatt gtgagcgctc acaattccac aacggtttcc ctctagaaat aattttgttt     300
aactttttgg aggagacaca atgacggcat tgactgaagg cgcaaaactg ttcgagaaag     360
aaatccccta catcaccgaa ctcgagggcg acgtagaggg catgaagttc atcatcaagg     420
gcgagggaac gggcgacgcc accaccggca ccatcaaggc gaagtatatc tgcaccacgg     480
gagatctgcc ggttccctgg gcgacgctgg tgtcgacctt gtcctacggc gtccaatgct     540
tcgcgaagta ccccagtcac atcaaagact tctttaagag cgccatgcca gaaggctaca     600
cgcaggaacg gaccatctcg ttcgaagggg atggggtcta caagacccgt gcgatggtga     660
cgtacgagcg cgggtccatc tacaaccggg tcactctgac cggtgagaac ttcaagaagg     720
acggccacat tctgcgcaag aacgtcgcct tccagtgtcc gccgtcgatc ctgtatatcc     780
tgccggatac cgtgaacaac ggtattcgcg tggaattcaa tcaggcctat gatatcgagg     840
gtgtcacgga gaagcttgtg accaaatgca gccagatgaa tcgccctctg gccggctccg     900
ccgctgtcca tatcccgcgg taccaccata tcacctatca taccaagctc agcaaagacc     960
gcgacgagag gcgggaccac atgtgcctcg tggaagtggt gaaggccgtc gacctggaca    1020
cctatcagtg a                                                         1031

<210> 35
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> first lacO sequence (lacO1) (in bold in SEQ ID
      NO:34)

<400> 35
aattgtgagc ggataacaat t                                                21

<210> 36
<211> 220
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative MDH, large subunit (MCA0779) promoter of
      SEQ ID NO:34.  The last 3 nucleotides  are a 5'
      portion of native ribosome binding site.

<400> 36
tttgcctcga tcggcggtcc ttgtgacagg gagatattcc cgacggatcc ggggcattcg      60
agcggaaccg cccgccgtgg gagtttttcc agcgagcatt cgagagtttt tcaaggcggc     120
ttcgaggggt tattccgtaa cgccgccgac atgatctgtc ccagaatctc cgccgctgtt     180
cgtagagcgc cgatgcaggg tcggcatcaa tcattcttgg                           220

<210> 37
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> second lacO sequence (lacO2) (in bold in SEQ ID
      NO:34)

<400> 37
tgtggaattg tgagcgctca caattccaca                                       30

<210> 38
<211> 711
<212> DNA
<213> Artificial Sequence

<220> 
<223> sequence encoding Dasher green fluorescent protein
      of SEQ ID NO:34

<400> 38
atgacggcat tgactgaagg cgcaaaactg ttcgagaaag aaatccccta catcaccgaa      60
ctcgagggcg acgtagaggg catgaagttc atcatcaagg gcgagggaac gggcgacgcc     120
accaccggca ccatcaaggc gaagtatatc tgcaccacgg gagatctgcc ggttccctgg     180
gcgacgctgg tgtcgacctt gtcctacggc gtccaatgct tcgcgaagta ccccagtcac     240
atcaaagact tctttaagag cgccatgcca gaaggctaca cgcaggaacg gaccatctcg     300
ttcgaagggg atggggtcta caagacccgt gcgatggtga cgtacgagcg cgggtccatc     360
tacaaccggg tcactctgac cggtgagaac ttcaagaagg acggccacat tctgcgcaag     420
aacgtcgcct tccagtgtcc gccgtcgatc ctgtatatcc tgccggatac cgtgaacaac     480
ggtattcgcg tggaattcaa tcaggcctat gatatcgagg gtgtcacgga gaagcttgtg     540
accaaatgca gccagatgaa tcgccctctg gccggctccg ccgctgtcca tatcccgcgg     600
taccaccata tcacctatca taccaagctc agcaaagacc gcgacgagag gcgggaccac     660
atgtgcctcg tggaagtggt gaaggccgtc gacctggaca cctatcagtg a              711

<210> 39
<211> 1083
<212> DNA
<213> Artificial Sequence

<220> 
<223> nucleotide sequence encoding wild-type lacI

<400> 39
atgaaaccag tgactttgta cgatgtagcg gaatatgcag gcgtaagcta ccagactgtg      60
tcccgtgtcg ttaaccaagc cagccacgtg agcgcgaaaa cgcgcgagaa ggttgaggca     120
gcaatggcgg aactgaatta cattccgaac cgtgttgctc aacagttggc cggcaagcag     180
agcctgctga ttggcgttgc gacctctagc ctggcgctgc acgcgagcag ccaaatcgtg     240
gcagcgatta agtctcgtgc ggatcaactg ggtgcgagcg tcgtggtgag catggtggaa     300
cgtagcggtg tcgaggcttg taaagcggca gttcacaatc tgctggccca gcgtgtcagc     360
ggcctgatta tcaattaccc gctggacgac caggacgcaa tcgctgttga ggcggcttgc     420
acgaatgtcc cagcactgtt cttggacgtt agcgatcaga ccccgattaa ctccatcatc     480
ttctcccatg aggacggcac ccgcctgggc gtcgaacatc tggttgcgct gggtcatcag     540
cagatcgcgc tgttggcggg tccgttgagc agcgtgagcg cgcgtctgcg tctggcgggc     600
tggcacaaat atctgacgcg caaccaaatc cagcctattg cggagcgcga aggtgattgg     660
agcgcaatgt ctggctttca gcaaacgatg cagatgctga atgaaggtat tgttccgacc     720
gcgatgctgg ttgttaacga tcagatggcc ctgggtgcga tgcgtgcaat caccgagagc     780
ggcctgcgcg ttggtgccga catttccgtc gtcggttatg acgacaccga ggatagctct     840
tgctacatcc caccgctgac caccattaaa caagattttc gtctgctggg tcaaaccagc     900
gtcgatcgcc tgctgcaatt gtcgcagggt caggccgtga aaggtaatca actgctgccg     960
gtgagcctgg ttaagcgtaa gacgacgttg gcaccgaaca cccaaaccgc aagcccgcgt    1020
gctctggccg acagcctgat gcaactggcg cgtcaagtgt cgcgcctgga aagcggtcag    1080
taa                                                                  1083

<210> 40
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> native MDH, large subunit (MCA0779) ribosome
      binding site with native spacer

<400> 40
tggaggagac ac                                                          12

<210> 41
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> modified MDH, large subunit (MCA0779) ribosome
      binding site with modified spacer of SEQ ID NO:34

<400> 41
tggaggagac aca                                                         13

<210> 42
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative promoter from the trfA-containing operon
      of plasmid RK2 (BN000925)

<400> 42
ttgacagcgg aaccaatgtt tagctaaact agagtctcct ttctcaagga gactttcgat      60

<210> 43
<211> 172
<212> DNA
<213> Artificial Sequence

<220> 
<223> putative Methylococcus capsulatus Bath 30S
      ribosomal protein S12 promoter with altered 
      MDH ribosomal binding sequence
      

<400> 43
aattccaaaa aaggcttgca gcgatgtgag cctttttatt tttgtttgtt cgggcgtaag      60
tgcgcttgac atggcggctg ccagtaatta cgctgggtag ctttggtctt tcccgtattt     120
atgttgcgcg gttctgtgcg tgatgcgggt tggaatggtt ttggaggaga ca             172

<210> 44
<211> 77
<212> DNA
<213> Artificial Sequence

<220> 
<223> > putative Methylococcus capsulatus Bath 50S
        ribosomal protein L13 promoter with altered 
        MDH ribosomal binding sequence

<400> 44
aaatgtgctt gtattccagt ggccgcttgg atagaattgc gcgctttgat tggggcgcca      60
gaacggtgga ggagaca                                                     77

<210> 45
<211> 315
<212> PRT
<213> Artificial Sequence

<220> 
<223> BenR transcriptional activator Protein_ABA06553.1
      (Pseudomonas fluorescens) |gi|74422787

<400> 45
Met Thr Val Leu Leu Ser Glu Arg Ser Gln Ile Phe Gln Gly Ala Asp
 1               5                  10                  15
Ala Tyr Ala Val Ser Asp Tyr Val Asn Gln His Val Gly Ser His Cys
            20                  25                  30
Ile Arg Leu Pro Pro Arg Gly Gln Pro Arg Ala Ser Ile Ser His Arg
        35                  40                  45
Thr Phe Ala Ser Leu Asp Leu Cys Arg Ile Ser Tyr Gly Ala Pro Val
    50                  55                  60
Arg Val Thr Ser Val Ala Leu Glu Thr Ile Tyr His Leu Gln Ile Leu
65                  70                  75                  80
Leu Ser Gly His Cys Arg Ser Asn Ser Arg Gly Glu Asp Asp Val Phe
                85                  90                  95
Gly Pro Gly Glu Ile Leu Leu Ile Asn Pro Asp Asp Pro Val Asp Leu
            100                 105                 110
Thr Tyr Ser Ala Asp Cys Glu Lys Phe Ile Ile Lys Leu Pro Val Arg
        115                 120                 125
Leu Leu Glu Asn Ala Cys Leu Glu Gln His Trp Ser Leu Pro Arg Ala
    130                 135                 140
Gly Val Arg Phe Thr Thr Ala Arg His Ala Leu Ser Glu Met Gly Gly
145                 150                 155                 160
Phe Leu Pro Leu Leu Gly Leu Ile Cys His Glu Ala Glu Asn Ala Ala
                165                 170                 175
Glu Pro His Met Gln Gly Leu Tyr Glu Arg Ile Val Ala Asn Lys Leu
            180                 185                 190
Leu Ala Leu Leu Gly Ser Asn Val Ser Arg Val Thr Pro Arg Ala Ala
        195                 200                 205
His Gly Gly Gly Phe Glu Ala Val His Glu Phe Ile Gln Gln His Leu
    210                 215                 220
Gly Asp Asp Ile Ser Val Glu Gln Leu Met Ala Val Ala Asn Val Ser
225                 230                 235                 240
Glu Arg Ser Leu Tyr Ser Leu Phe Glu Arg Gln Val Gly Leu Ser Pro
                245                 250                 255
Arg Asp Tyr Val Arg Arg Cys Lys Leu Glu Arg Val His Ala Arg Leu
            260                 265                 270
Gln Leu Ser Ser Thr Arg Ser Val Thr Glu Val Ala Leu Asp His Gly
        275                 280                 285
Phe Met His Leu Gly Arg Phe Ser Glu Ala Tyr Arg Lys Arg Phe Gly
    290                 295                 300
Glu Leu Pro Ser Gln Thr Trp Lys Arg His Arg
305                 310                 315

<210> 46
<211> 948
<212> DNA
<213> Pseudomonas fluorescens

<400> 46
atgaccgtgc tattgagtga gcgcagccag attttccagg gcgccgatgc ctacgcggtg      60
tcggactacg tcaaccagca tgtgggcagc cactgcattc gcctgcctcc caggggccag     120
ccccgggcaa gtatcagcca tcgcaccttc gccagcctgg acctgtgccg catcagctac     180
ggcgcaccgg tgcgggtcac gtcggtggcg ctggagacca tctaccacct gcagatcctc     240
ttgagcgggc attgccgctc caactcccgt ggcgaggatg atgtgttcgg gccgggggaa     300
atcctgctga tcaatccgga cgacccggta gacctgacct attccgccga ctgcgaaaaa     360
ttcatcatca aactgccggt gcgcctgctg gaaaacgcct gcctggagca gcactggagc     420
ctgccgcggg cgggggtccg cttcacgacg gcccgccacg cgctcagtga aatgggcggc     480
ttcctgccgt tgctcgggtt gatctgccat gaggcggaaa acgctgccga gccccacatg     540
caaggcctgt acgaacgcat cgtggccaac aagctgctgg cattgctggg cagcaatgtg     600
tcgcgggtga ccccccgggc tgcccacggc ggtgggtttg aagcggtgca cgaatttatc     660
cagcagcacc tgggcgatga catcagcgtc gagcagttga tggccgtggc caacgtcagt     720
gaacgttcgc tgtacagcct gtttgagcgc caggtggggc tgtcgccgcg cgattacgta     780
cgccgctgca agctcgaacg cgtacatgca cgcttgcaac taagcagcac gcgcagcgtg     840
accgaggtgg ctttggacca tgggttcatg cacctagggc ggttttccga agcctatcgc     900
aaacgcttcg gcgaactgcc gtcgcagacc tggaaacgcc atcgttaa                  948

<210> 47
<211> 948
<212> DNA
<213> Artificial Sequence

<220> 
<223> benR codon optimized for Methylococcus capsulatus
      Bath

<400> 47
atgaccgtcc ttttgagtga acgcagccaa atttttcagg gagccgatgc ctatgccgtc      60
agtgactacg tcaaccaaca tgtgggctcg cactgcatca ggctgccacc gcggggccag     120
ccgcgggcga gcatctcgca ccgcaccttc gccagcctgg acctgtgccg catctcgtac     180
ggcgcacccg tccgcgtcac ctcggttgcg ctcgaaacga tctaccatct gcagatcctg     240
ttgtccggcc actgccggtc caattcgcgc ggtgaggatg acgtgtttgg gcccggcgag     300
atcctgctca ttaatccgga cgacccggtc gacctgacct acagcgcgga ttgcgagaaa     360
ttcataatca agctgccggt gcgcctcctt gagaacgcct gcttggagca gcattggtcg     420
ctgccgaggg ccggcgtccg cttcaccacg gcacggcatg cgctgagcga gatgggcggc     480
ttcctcccgc tcctcggtct gatctgtcac gaagccgaga acgctgccga gccgcacatg     540
caaggcctgt acgagcgcat cgtggcgaac aagctgctgg cgctgctggg atccaatgtg     600
tcccgcgtga cgccccgggc ggcgcatggt ggcggcttcg aagccgtcca tgagttcatc     660
cagcagcatc tcggcgacga catctccgtc gaacagctca tggctgtcgc gaacgtgtcc     720
gagcgctcgc tttattccct cttcgagcgc caggtcgggc tgagccccag ggactatgtg     780
cggcgctgca agctcgaacg cgttcatgcc cggctccagc tctcgtccac ccgcagcgtg     840
accgaagtgg ccctcgacca tggcttcatg cacctcggcc ggttctccga ggcgtatcgc     900
aaacggttcg gtgagctgcc ttcgcaaacc tggaagcgcc accggtag                  948

<210> 48
<211> 107
<212> DNA
<213> Artificial Sequence

<220> 
<223> Putative ben promoter and ribosomal binding site
      with spacer from DQ172832.1: 1009-1115
      (Pseudomonas fluorescens); 107 bp intergenic
      region between benR and benA

<400> 48
gcgacgtgcg cctggcggat agcgatgtgc aggcagcgga tattgacggg cagggcgagc      60
acgtacggtg agggcgcctg atacaagaac aacggagggc ccgcccc                   107

<210> 49
<211> 321
<212> PRT
<213> Pseudomonas putida plasmid pWW0

<400> 49
Met Asp Phe Cys Leu Leu Asn Glu Lys Ser Gln Ile Phe Val His Ala
 1               5                  10                  15
Glu Pro Tyr Ala Val Ser Asp Tyr Val Asn Gln Tyr Val Gly Thr His
            20                  25                  30
Ser Ile Arg Leu Pro Lys Gly Gly Arg Pro Ala Gly Arg Leu His His
        35                  40                  45
Arg Ile Phe Gly Cys Leu Asp Leu Cys Arg Ile Ser Tyr Gly Gly Ser
    50                  55                  60
Val Arg Val Ile Ser Pro Gly Leu Glu Thr Cys Tyr His Leu Gln Ile
65                  70                  75                  80
Ile Leu Lys Gly His Cys Leu Trp Arg Gly His Gly Gln Glu His Tyr
                85                  90                  95
Phe Ala Pro Gly Glu Leu Leu Leu Leu Asn Pro Asp Asp Gln Ala Asp
            100                 105                 110
Leu Thr Tyr Ser Glu Asp Cys Glu Lys Phe Ile Val Lys Leu Pro Ser
        115                 120                 125
Val Val Leu Asp Arg Ala Cys Ser Asp Asn Asn Trp His Lys Pro Arg
    130                 135                 140
Glu Gly Ile Arg Phe Ala Ala Arg His Asn Leu Gln Gln Leu Asp Gly
145                 150                 155                 160
Phe Ile Asn Leu Leu Gly Leu Val Cys Asp Glu Ala Glu His Thr Lys
                165                 170                 175
Ser Met Pro Arg Val Gln Glu His Tyr Ala Gly Ile Ile Ala Ser Lys
            180                 185                 190
Leu Leu Glu Met Leu Gly Ser Asn Val Ser Arg Glu Ile Phe Ser Lys
        195                 200                 205
Gly Asn Pro Ser Phe Glu Arg Val Val Gln Phe Ile Glu Glu Asn Leu
    210                 215                 220
Lys Arg Asn Ile Ser Leu Glu Arg Leu Ala Glu Leu Ala Met Met Ser
225                 230                 235                 240
Pro Arg Ser Leu Tyr Asn Leu Phe Glu Lys His Ala Gly Thr Thr Pro
                245                 250                 255
Lys Asn Tyr Ile Arg Asn Arg Lys Leu Glu Ser Ile Arg Ala Cys Leu
            260                 265                 270
Asn Asp Pro Ser Ala Asn Val Arg Ser Ile Thr Glu Ile Ala Leu Asp
        275                 280                 285
Tyr Gly Phe Leu His Leu Gly Arg Phe Ala Glu Asn Tyr Arg Ser Ala
    290                 295                 300
Phe Gly Glu Leu Pro Ser Asp Thr Leu Arg Gln Cys Lys Lys Glu Val
305                 310                 315                 320
Ala


<210> 50
<211> 966
<212> DNA
<213> Pseudomonas putida plasmid pWW0

<400> 50
atggattttt gcttattgaa cgagaaaagt cagatcttcg tccacgccga gccctatgca      60
gtctccgatt atgttaacca gtatgtcggt acgcactcta ttcgcctgcc caagggcggg     120
cgcccggcag gcaggctgca ccacagaatc ttcggatgcc tcgacctgtg tcgaatcagc     180
tacggcggta gcgtgagggt aatctcgcct ggattagaga cctgttatca tctgcaaata     240
atactcaaag gccattgcct gtggcgtggc catggccagg agcactattt tgcgccgggc     300
gaactattgc tgctcaatcc ggatgaccaa gccgacctga cctattcaga agattgcgag     360
aaatttatcg ttaaattgcc ctcagtggtc cttgatcggg catgcagtga caacaattgg     420
cacaagccga gggagggtat ccgtttcgcc gcgcgacaca atctccagca actcgatggc     480
tttatcaatc tactcgggtt agtttgtgac gaagcggaac atacaaagtc gatgcctcgg     540
gtccaagagc actatgcggg gatcatcgct tccaagctgc tcgaaatgct gggcagcaat     600
gtcagccgtg aaattttcag caaaggtaac ccgtctttcg agcgagtcgt tcaattcatt     660
gaggagaatc tcaaacggaa tatcagcctt gagcggttag cggagctggc gatgatgagt     720
ccacgctcgc tctacaattt gttcgagaag catgccggca ccacgccgaa gaactacatc     780
cgcaaccgca agctcgaaag catccgcgcc tgcttgaacg atcccagtgc caatgtgcgt     840
agtataactg agatagccct agactacggc ttcttacatt tgggacgctt cgctgaaaac     900
tataggagcg cgttcggcga gttgccttcc gacaccctgc gtcaatgcaa aaaggaagtg     960
gcttga                                                                966

<210> 51
<211> 966
<212> DNA
<213> Artificial Sequence

<220> 
<223> xylS codon optimized for Methylococcus capsulatus
      Bath

<400> 51
atggacttct gcctgcttaa tgaaaaatcc cagatctttg tccacgccga gccgtacgcc      60
gtgagcgact atgtgaacca atatgttggt acccattcga tccggctccc gaagggtggc     120
cgcccggcgg gcaggctcca tcaccgcata ttcggctgcc tcgatctctg ccgcatctcg     180
tacggcggca gcgtccgggt gatctcgccg ggtctggaaa cgtgctatca tctgcagatc     240
attctcaagg gccactgcct ctggcgggga catggccagg aacattactt cgcccctgga     300
gagctgctgc tcttgaaccc ggacgatcag gcagatttga cctactccga ggactgtgag     360
aagttcatcg tgaagctgcc cagcgtcgtc ctcgaccgcg cgtgctcgga taacaactgg     420
cataagccac gcgagggcat ccggttcgcg gcacggcaca atctgcaaca gcttgacggg     480
ttcatcaatc tcctgggcct cgtctgcgac gaagccgagc acactaagtc gatgcccagg     540
gtccaagagc attatgcggg catcatcgcg tccaagctgc tcgaaatgct cgggtccaac     600
gtgagccgcg agattttcag taagggcaac cccagcttcg agcgcgtcgt gcagttcatc     660
gaagagaacc tgaagcgcaa tatctccctg gagcgcctcg ccgagcttgc catgatgagt     720
ccgcgcagcc tctataacct gtttgagaaa catgcgggca ccacgcccaa gaattacatt     780
cgcaaccgca aactggagtc catccgcgct tgcctcaacg acccgtcggc gaatgtccgg     840
tcgatcaccg agatcgccct ggactacggt ttcctgcacc tgggccggtt cgccgagaat     900
tatcgctccg ctttcggcga gctgccgtcg gacaccctgc gccagtgcaa aaaagaagtg     960
gcgtga                                                                966

<210> 52
<211> 62
<212> DNA
<213> Artificial Sequence

<220> 
<223> Putative xylX promoter (Pm) and ribosomal binding
      site from pWW0 (Pseudomonas putida); 62 bp region
      upstream of xylX translational start site

<400> 52
ggctatctct agaaaggcct accccttagg ctttatgcaa cagaaacaat aataatggag      60
tc                                                                     62

<210> 53
<211> 6
<212> DNA
<213> Artificial Sequence

<220> 
<223> Consensus RBS

<400> 53
aggagg                                                                  6

<210> 54
<211> 14
<212> DNA
<213> Artificial Sequence

<220> 
<223> benA ribosomal binding site with spacer from
      DQ172832.1: 1009-1115 (Pseudomonas fluorescens)

<400> 54
ggagggcccg cccc                                                        14
