                         SEQUENCE LISTING

<110>  Norwegian Institute for Agricultural & Environmental Research
 
<120>  Transgenic plants expressing a tetravalent chimeric dengue virus 
       antigen to produce effective vaccines derived therefrom

<130>  P61401621NO00

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  116
<212>  PRT
<213>  Dengue virus

<400>  1

Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 
1               5                   10                  15      


Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 
            20                  25                  30          


Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Ser Gln Asp Glu Lys Gly 
        35                  40                  45              


Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 
    50                  55                  60                  


Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 
65                  70                  75                  80  


Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 
                85                  90                  95      


Lys Lys Gly Ser Ser Ile Gly Lys Gly Gly Gly Gly Gly Glu Asn Leu 
            100                 105                 110         


Tyr Phe Gln Gly 
        115     


<210>  2
<211>  116
<212>  PRT
<213>  Dengue virus

<400>  2

Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile 
1               5                   10                  15      


Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val Gln Tyr Glu Gly 
            20                  25                  30          


Asp Gly Ser Pro Cys Lys Thr Pro Phe Glu Ile Met Asp Leu Glu Lys 
        35                  40                  45              


Arg His Val Leu Gly Arg Leu Thr Thr Val Asn Pro Ile Val Thr Glu 
    50                  55                  60                  


Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser 
65                  70                  75                  80  


Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys Leu Asp Trp Phe 
                85                  90                  95      


Lys Lys Gly Ser Ser Ile Gly Gln Gly Gly Gly Gly Gly Glu Asn Leu 
            100                 105                 110         


Tyr Phe Gln Gly 
        115     


<210>  3
<211>  116
<212>  PRT
<213>  Dengue virus

<400>  3

Met Ser Tyr Ala Met Cys Leu Asn Thr Phe Val Leu Lys Lys Glu Val 
1               5                   10                  15      


Ser Glu Thr Gln His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly 
            20                  25                  30          


Glu Asp Ala Pro Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly 
        35                  40                  45              


Lys Ala His Asn Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys 
    50                  55                  60                  


Lys Glu Glu Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 
65                  70                  75                  80  


Asn Ile Val Ile Gly Ile Gly Asp Lys Ala Leu Lys Ile Asn Trp Tyr 
                85                  90                  95      


Arg Lys Gly Ser Ser Ile Gly Lys Gly Gly Gly Gly Gly Glu Asn Leu 
            100                 105                 110         


Tyr Phe Gln Gly 
        115     


<210>  4
<211>  115
<212>  PRT
<213>  Dengue virus

<400>  4

Met Ser Tyr Thr Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met 
1               5                   10                  15      


Ala Glu Thr Gln His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly 
            20                  25                  30          


Ala Gly Ala Pro Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys 
        35                  40                  45              


Glu Lys Val Val Gly Arg Ile Ile Ser Pro Thr Pro Phe Ala Glu Asn 
    50                  55                  60                  


Thr Asn Ser Val Thr Asn Ile Glu Leu Glu Arg Pro Leu Asp Ser Tyr 
65                  70                  75                  80  


Ile Val Ile Gly Val Gly Asp Ser Ala Leu Thr Leu His Trp Phe Arg 
                85                  90                  95      


Lys Gly Ser Ser Ile Gly Lys Gly Gly Gly Gly Gly Glu Asn Leu Tyr 
            100                 105                 110         


Phe Gln Gly 
        115 


<210>  5
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric tetravalent dengue virus antigen

<400>  5

Met Ser Tyr Val Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val 
1               5                   10                  15      


Ala Glu Thr Gln His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly 
            20                  25                  30          


Thr Asp Ala Pro Cys Lys Ile Pro Phe Ser Ser Gln Asp Glu Lys Gly 
        35                  40                  45              


Val Thr Gln Asn Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp 
    50                  55                  60                  


Lys Glu Lys Pro Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser 
65                  70                  75                  80  


Tyr Ile Val Val Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe 
                85                  90                  95      


Lys Lys Gly Ser Ser Ile Gly Lys Gly Gly Gly Gly Gly Glu Asn Leu 
            100                 105                 110         


Tyr Phe Gln Gly Met Ser Tyr Ser Met Cys Thr Gly Lys Phe Lys Val 
        115                 120                 125             


Val Lys Glu Ile Ala Glu Thr Gln His Gly Thr Ile Val Ile Arg Val 
    130                 135                 140                 


Gln Tyr Glu Gly Asp Gly Ser Pro Cys Lys Thr Pro Phe Glu Ile Met 
145                 150                 155                 160 


Asp Leu Glu Lys Arg His Val Leu Gly Arg Leu Thr Thr Val Asn Pro 
                165                 170                 175     


Ile Val Thr Glu Lys Asp Ser Pro Val Asn Ile Glu Ala Glu Pro Pro 
            180                 185                 190         


Phe Gly Asp Ser Tyr Ile Ile Ile Gly Val Glu Pro Gly Gln Leu Lys 
        195                 200                 205             


Leu Asp Trp Phe Lys Lys Gly Ser Ser Ile Gly Gln Gly Gly Gly Gly 
    210                 215                 220                 


Gly Glu Asn Leu Tyr Phe Gln Gly Met Ser Tyr Ala Met Cys Leu Asn 
225                 230                 235                 240 


Thr Phe Val Leu Lys Lys Glu Val Ser Glu Thr Gln His Gly Thr Ile 
                245                 250                 255     


Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp Ala Pro Cys Lys Ile Pro 
            260                 265                 270         


Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala His Asn Gly Arg Leu Ile 
        275                 280                 285             


Thr Ala Asn Pro Val Val Thr Lys Lys Glu Glu Pro Val Asn Ile Glu 
    290                 295                 300                 


Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile Val Ile Gly Ile Gly Asp 
305                 310                 315                 320 


Lys Ala Leu Lys Ile Asn Trp Tyr Arg Lys Gly Ser Ser Ile Gly Lys 
                325                 330                 335     


Gly Gly Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly Met Ser Tyr Thr 
            340                 345                 350         


Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala Glu Thr Gln 
        355                 360                 365             


His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala Gly Ala Pro 
    370                 375                 380                 


Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Glu Lys Val Val 
385                 390                 395                 400 


Gly Arg Ile Ile Ser Pro Thr Pro Phe Ala Glu Asn Thr Asn Ser Val 
                405                 410                 415     


Thr Asn Ile Glu Leu Glu Arg Pro Leu Asp Ser Tyr Ile Val Ile Gly 
            420                 425                 430         


Val Gly Asp Ser Ala Leu Thr Leu His Trp Phe Arg Lys Gly Ser Ser 
        435                 440                 445             


Ile Gly Lys Gly Gly Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly 
    450                 455                 460             


<210>  6
<211>  1392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence coding for chimeric tetravalent dengue virus 
       antigen

<400>  6
atgtcttatg taatgtgtac tggttctttt aaattagaaa aagaagtagc tgaaactcaa       60

catggaactg tattagtaca agtaaaatat gaaggtactg atgctccttg taaaattcct      120

ttttcttctc aagatgaaaa aggtgtaact caaaatggaa gacttattac tgctaatcct      180

attgttactg ataaagaaaa acctgtaaat attgaagctg aacctccttt tggtgaatct      240

tatattgttg taggtgctgg tgaaaaagca ttaaaattat cttggtttaa aaaaggttct      300

tctattggaa aaggaggtgg tggaggagaa aatttatatt ttcaaggtat gtcttattct      360

atgtgtactg gaaaatttaa agttgtaaaa gaaattgctg aaactcaaca tggaactatt      420

gtaattcgag ttcaatatga aggagatggt tctccttgta aaactccttt tgaaattatg      480

gatttagaaa aaagacatgt tttaggacga ttaactactg taaatcctat tgttactgaa      540

aaagattctc ctgtaaatat tgaagctgaa cctccttttg gtgattctta tattattatt      600

ggtgtagaac ctggacaatt aaaattagat tggtttaaaa aaggatcttc tattggtcaa      660

ggaggtggtg gaggagaaaa tttatatttt caaggtatgt cttatgctat gtgtttaaat      720

acttttgttt taaaaaaaga agtttctgaa actcaacatg gaactattct tattaaagtt      780

gaatataaag gtgaagatgc tccttgtaaa attccttttt ctactgaaga tggacaagga      840

aaagctcata atggaagatt aataactgct aatcctgttg taactaaaaa agaagaacct      900

gttaatattg aagctgaacc tccttttggt gaatctaata ttgtaattgg tattggagat      960

aaagcattaa aaataaattg gtatagaaaa ggttcttcta ttggtaaagg aggtggtgga     1020

ggagaaaatt tatattttca aggtatgtct tatactatgt gttctggaaa attttctatt     1080

gataaagaaa tggctgaaac tcaacatgga actactgtag ttaaagtaaa atatgaaggt     1140

gctggagcac cttgtaaagt acctattgaa attcgagatg ttaataaaga aaaagtagta     1200

ggaagaatta tttctcctac tccttttgct gaaaatacta attctgtaac taatattgaa     1260

ttagaaagac ctttagattc ttatattgta attggagtag gagattctgc tttaacttta     1320

cattggttta gaaaaggatc ttctattgga aaaggaggtg gtggaggaga aaatttatat     1380

tttcaaggtt aa                                                         1392


<210>  7
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized sequence (EDIII-1)

<400>  7
atgtcttatg taatgtgtac tggttctttt aaattagaaa aagaagtagc tgaaactcaa       60

catggaactg tattagtaca agtaaaatat gaaggtactg atgctccttg taaaattcct      120

ttttcttctc aagatgaaaa aggtgtaact caaaatggaa gacttattac tgctaatcct      180

attgttactg ataaagaaaa acctgtaaat attgaagctg aacctccttt tggtgaatct      240

tatattgttg taggtgctgg tgaaaaagca ttaaaattat cttggtttaa aaaaggttct      300

tctattggaa aaggaggtgg tggaggagaa aatttatatt ttcaaggt                   348


<210>  8
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized sequence (EDIII-2)

<400>  8
atgtcttatt ctatgtgtac tggaaaattt aaagttgtaa aagaaattgc tgaaactcaa       60

catggaacta ttgtaattcg agttcaatat gaaggagatg gttctccttg taaaactcct      120

tttgaaatta tggatttaga aaaaagacat gttttaggac gattaactac tgtaaatcct      180

attgttactg aaaaagattc tcctgtaaat attgaagctg aacctccttt tggtgattct      240

tatattatta ttggtgtaga acctggacaa ttaaaattag attggtttaa aaaaggatct      300

tctattggtc aaggaggtgg tggaggagaa aatttatatt ttcaaggt                   348


<210>  9
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized sequence (EDIII-3)

<400>  9
atgtcttatg ctatgtgttt aaatactttt gttttaaaaa aagaagtttc tgaaactcaa       60

catggaacta ttcttattaa agttgaatat aaaggtgaag atgctccttg taaaattcct      120

ttttctactg aagatggaca aggaaaagct cataatggaa gattaataac tgctaatcct      180

gttgtaacta aaaaagaaga acctgttaat attgaagctg aacctccttt tggtgaatct      240

aatattgtaa ttggtattgg agataaagca ttaaaaataa attggtatag aaaaggttct      300

tctattggta aaggaggtgg tggaggagaa aatttatatt ttcaaggt                   348


<210>  10
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized sequence (EDIII-4)

<400>  10
atgtcttata ctatgtgttc tggaaaattt tctattgata aagaaatggc tgaaactcaa       60

catggaacta ctgtagttaa agtaaaatat gaaggtgctg gagcaccttg taaagtacct      120

attgaaattc gagatgttaa taaagaaaaa gtagtaggaa gaattatttc tcctactcct      180

tttgctgaaa atactaattc tgtaactaat attgaattag aaagaccttt agattcttat      240

attgtaattg gagtaggaga ttctgcttta actttacatt ggtttagaaa aggatcttct      300

attggaaaag gaggtggtgg aggagaaaat ttatattttc aaggt                      345


<210>  11
<211>  15
<212>  PRT
<213>  Dengue virus

<400>  11

Lys Phe Lys Val Val Lys Glu Ile Ala Glu Thr Gln His Gly Thr 
1               5                   10                  15  


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  12
acccatggct tctaaaggag                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  13
agacagcgac gggttctctg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  14
gatccgagcc atagaatttc                                                   20


<210>  15
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  15
tgctggccgt acatttgtac g                                                 21


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  16
tacccgggaa ttgtgacctc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucletide Primer

<400>  17
agagtccgac cacaacgacc                                                   20


<210>  18
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucletide Primer

<400>  18
gctgaaactc aacatggaac tg                                                22


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  19
atgctttttc accagcacct                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  20
ttgctgaaac tcaacatgga                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  21
ccaaaaggag gttcagcttc                                                   20


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide primer

<400>  22
tgaagatgga caaggaaaag c                                                 21


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  23
ctccaccacc tcctttacca                                                   20


<210>  24
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  24
actactcaag ctgcattata tacc                                              24


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  25
gcacctttta ctaagatcaa tg                                                22


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  26
ggaggtagga tgggcagttg                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  27
ggactcgaac cgctgacatc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  28
ggactcgaac cgctgacatc                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  29
aacgaccttt tggaaacttc                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  30
tctgtgagcg tgacggtggt                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  31
ttacgcgaac gcgaagtccg                                                   20


<210>  32
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  32
gtaaaacgac ggccag                                                       16


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  33
caggaaacag ctatgacc                                                     18


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  34
tgacttatat actcgtgtca ac                                                22


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide Primer

<400>  35
ctgctaatgt ctactgtttg t                                                 21


