                         SEQUENCE LISTING

<110>  Biogen MA Inc.
       Tan, Siyuan
       Peters, Robert T.
       Liu, Tongyao
 
<120>  Optimized Factor IX Gene

<130>  2159.451PC02/C-K

<140>  To Be Assigned
<141>  2015-06-30

<150>  62/168,565
<151>  2015-05-29

<150>  62/019,069
<151>  2014-06-30


<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  1389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimized human factor IX nucleotide sequence

<400>  1
atgcagagag tcaacatgat tatggctgag tcacctgggc tgattactat ttgcctgctg       60

ggctacctgc tgtccgccga gtgtaccgtg ttcctggacc atgagaacgc aaataagatc      120

ctgaacaggc ccaaaagata caatagtggg aagctggagg aatttgtgca gggcaacctg      180

gagagagaat gcatggagga aaagtgtagc ttcgaggaag cccgcgaggt gtttgaaaat      240

acagagcgaa ccacagagtt ctggaagcag tatgtggacg gcgatcagtg cgagagcaac      300

ccctgtctga atggcggaag ttgcaaagac gatatcaact catacgaatg ctggtgtcct      360

ttcgggtttg aaggcaaaaa ttgcgagctg gacgtgacat gtaacattaa gaatggacgg      420

tgcgagcagt tttgtaaaaa ctctgccgat aataaggtgg tgtgcagctg tactgaagga      480

tatcgcctgg ctgagaacca gaagtcctgc gaaccagcag tgcccttccc ttgtgggagg      540

gtgagcgtct cccagacttc aaaactgacc agagcagaga cagtgtttcc cgacgtggat      600

tacgtcaaca gcactgaggc cgaaaccatc ctggacaaca ttactcagtc tacccagagt      660

ttcaatgact ttactcgggt ggtcgggggc gaggatgcta aaccaggcca gttcccctgg      720

caggtggtcc tgaacggaaa ggtggatgca ttttgcggag ggtctatcgt gaatgagaaa      780

tggattgtca ccgccgctca ctgcgtggaa accggagtca agatcacagt ggtcgctggg      840

gagcacaaca ttgaggaaac agaacatact gagcagaagc ggaatgtgat ccgcatcatt      900

cctcaccata actacaatgc agccatcaac aaatacaatc atgacattgc cctgctggaa      960

ctggatgagc ctctggtgct gaacagctac gtcactccaa tctgcattgc tgacaaagag     1020

tataccaata tcttcctgaa gtttggatca gggtacgtga gcggctgggg aagagtcttc     1080

cacaagggca ggagcgccct ggtgctccag tatctgcgag tgcctctggt cgatcgagct     1140

acctgtctga ggtctaccaa gtttacaatc tacaacaaca tgttctgcgc tgggtttcac     1200

gagggaggac gagactcctg tcagggcgat tctgggggcc cacatgtgac agaggtcgaa     1260

ggcaccagct tcctgactgg catcatttcc tggggagagg aatgtgcaat gaagggaaaa     1320

tacgggatct acaccaaagt gagccgctat gtgaactgga tcaaggaaaa aaccaaactg     1380

acctaatga                                                             1389


<210>  2
<211>  1389
<212>  DNA
<213>  Homo sapiens

<400>  2
atgcagcgcg tgaacatgat catggcagaa tcaccaggcc tcatcaccat ctgcctttta       60

ggatatctac tcagtgctga atgtacagtt tttcttgatc atgaaaacgc caacaaaatt      120

ctgaatcggc caaagaggta taattcaggt aaattggaag agtttgttca agggaatcta      180

gagagagaat gtatggaaga aaagtgtagt tttgaagaag cacgagaagt ttttgaaaac      240

actgaaagaa caactgaatt ttggaagcag tatgttgatg gagatcagtg tgagtccaat      300

ccatgtttaa atggcggcag ttgcaaggat gacattaatt cctatgaatg ttggtgtccc      360

tttggatttg aaggaaagaa ctgtgaatta gatgtaacat gtaacattaa gaatggcaga      420

tgcgagcagt tttgtaaaaa tagtgctgat aacaaggtgg tttgctcctg tactgaggga      480

tatcgacttg cagaaaacca gaagtcctgt gaaccagcag tgccatttcc atgtggaaga      540

gtttctgttt cacaaacttc taagctcacc cgtgctgaga ctgtttttcc tgatgtggac      600

tatgtaaatt ctactgaagc tgaaaccatt ttggataaca tcactcaaag cacccaatca      660

tttaatgact tcactcgggt tgttggtgga gaagatgcca aaccaggtca attcccttgg      720

caggttgttt tgaatggtaa agttgatgca ttctgtggag gctctatcgt taatgaaaaa      780

tggattgtaa ctgctgccca ctgtgttgaa actggtgtta aaattacagt tgtcgcaggt      840

gaacataata ttgaggagac agaacataca gagcaaaagc gaaatgtgat tcgaattatt      900

cctcaccaca actacaatgc agctattaat aagtacaacc atgacattgc ccttctggaa      960

ctggacgaac ccttagtgct aaacagctac gttacaccta tttgcattgc tgacaaggaa     1020

tacacgaaca tcttcctcaa atttggatct ggctatgtaa gtggctgggg aagagtcttc     1080

cacaaaggga gatcagcttt agttcttcag taccttagag ttccacttgt tgaccgagcc     1140

acatgtcttc gatctacaaa gttcaccatc tataacaaca tgttctgtgc tggcttccat     1200

gaaggaggta gagattcatg tcaaggagat agtgggggac cccatgttac tgaagtggaa     1260

gggaccagtt tcttaactgg aattattagc tggggtgaag agtgtgcaat gaaaggcaaa     1320

tatggaatat ataccaaggt atcccggtat gtcaactgga ttaaggaaaa aacaaagctc     1380

acttgataa                                                             1389


<210>  3
<211>  461
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SIGNAL
<222>  (1)..(28)
<223>  FIX Signal Peptide

<220>
<221>  PROPEP
<222>  (29)..(46)
<223>  FIX Propeptide

<400>  3

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Leu 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
    450                 455                 460     


<210>  4
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Potential Splice Site

<400>  4
ggtgat                                                                   6


<210>  5
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Potential Splice Site

<400>  5
ggtaag                                                                   6


<210>  6
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Destabilizing Sequence

<400>  6
attta                                                                    5


<210>  7
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly-T Sequence

<400>  7
tttttt                                                                   6


<210>  8
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly-A Sequence

<400>  8
aaaaaaa                                                                  7


<210>  9
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly-A Sequence

<400>  9
aataaa                                                                   6


<210>  10
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly-A Sequence

<400>  10
attaaa                                                                   6


<210>  11
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antiviral motif

<400>  11
tgtgt                                                                    5


<210>  12

<400>  12
000

<210>  13

<400>  13
000

<210>  14
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AU Rich Sequence Elements (ARE)

<400>  14
attttatt                                                                 8


<210>  15
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AU Rich Sequence Elements (ARE)

<400>  15
atttttaa                                                                 8


<210>  16

<400>  16
000

<210>  17
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP peptide

<400>  17

Asp Pro Arg Phe Gln Asp Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser 
1               5                   10                  15      


Leu Pro Ser Pro Ser Arg Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu 
            20                  25                  30          


<210>  18
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTP peptide

<400>  18

Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 
1               5                   10                  15      


Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln 
            20                  25              


<210>  19
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  albumin-binding peptides core sequence

<400>  19

Asp Ile Cys Leu Pro Arg Trp Gly Cys Leu Trp 
1               5                   10      


<210>  20
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  20

Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ser Ala Pro Ala 
            20  


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  21

Ala Ala Pro Ala Ser Pro Ala Pro Ala Ala Pro Ser Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ser 
            20  


<210>  22
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  22

Ala Pro Ser Ser Pro Ser Pro Ser Ala Pro Ser Ser Pro Ser Pro Ala 
1               5                   10                  15      


Ser Pro Ser Ser 
            20  


<210>  23
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  23

Ala Pro Ser Ser Pro Ser Pro Ser Ala Pro Ser Ser Pro Ser Pro Ala 
1               5                   10                  15      


Ser Pro Ser 
            


<210>  24
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  24

Ser Ser Pro Ser Ala Pro Ser Pro Ser Ser Pro Ala Ser Pro Ser Pro 
1               5                   10                  15      


Ser Ser Pro Ala 
            20  


<210>  25
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  25

Ala Ala Ser Pro Ala Ala Pro Ser Ala Pro Pro Ala Ala Ala Ser Pro 
1               5                   10                  15      


Ala Ala Pro Ser Ala Pro Pro Ala 
            20                  


<210>  26
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PAS Sequence

<400>  26

Ala Ser Ala Ala Ala Pro Ala Ala Ala Ser Ala Ala Ala Ser Ala Pro 
1               5                   10                  15      


Ser Ala Ala Ala 
            20  


<210>  27

<400>  27
000

<210>  28

<400>  28
000

<210>  29
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 amino acids 233-236

<400>  29

Glu Leu Leu Gly 
1               


<210>  30
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE42-4

<400>  30

Gly Ala Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly 
1               5                   10                  15      


Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala 
            20                  25                  30          


Thr Ser Gly Ser Glu Thr Pro Ala Ser Ser 
        35                  40          


<210>  31
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE42-4

<400>  31
ggcgcgccag gttctcctgc tggctccccc acctcaacag aagaggggac aagcgaaagc       60

gctacgcctg agagtggccc tggctctgag ccagccacct ccggctctga aacccctgcc      120

tcgagc                                                                 126


<210>  32
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-2A

<400>  32

Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Ser Pro Ala Gly 
1               5                   10                  15      


Ser Pro Thr Ser Thr Glu Glu Gly Thr Ser Thr Glu Pro Ser Glu Gly 
            20                  25                  30          


Ser Ala Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
        35                  40                  45              


Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Thr Ser Thr Glu 
    50                  55                  60                  


Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
65                  70                  75                  80  


Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly 
                85                  90                  95      


Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Thr Glu 
            100                 105                 110         


Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        115                 120                 125             


Ser Gly Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly 
    130                 135                 140                 


<210>  33
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-2A

<400>  33
ggcgcgccaa ccagtacgga gccgtccgag gggagcgcac caggaagccc ggctgggagc       60

ccgacttcta ccgaagaggg tacatctacc gaaccaagtg aaggttcagc accaggcacc      120

tcaacagaac cctctgaggg ctcggcgcct ggtacaagtg agtccgccac cccagaatcc      180

gggcctggga caagcacaga accttcggaa gggagtgccc ctggaacatc cgaatcggca      240

accccagaat cagggccagg atctgagccc gcgacttcgg gctccgagac gcctgggaca      300

tccaccgagc cctccgaagg atcagcccca ggcaccagca cggagccctc tgagggaagc      360

gcacctggta ccagcgaaag cgcaactccc gaatcaggtc ccggtacgag cgagtcggcg      420

accccggaga gcgggccagg tgcctcgagc                                       450


<210>  34
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-3B

<400>  34

Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly Thr Ser Glu Ser 
1               5                   10                  15      


Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser Gly Ser 
            20                  25                  30          


Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly 
        35                  40                  45              


Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Thr Glu 
    50                  55                  60                  


Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly 
65                  70                  75                  80  


Ser Ala Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
                85                  90                  95      


Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Thr Glu 
            100                 105                 110         


Pro Ser Glu Gly Ser Ala Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser 
        115                 120                 125             


Thr Glu Glu Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
    130                 135                 140                 


<210>  35
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-3B

<400>  35
ggcgcgccaa gtcccgctgg aagcccaact agcaccgaag aggggacctc agagtccgcc       60

acccccgagt ccggccctgg ctctgagcct gccactagcg gctccgagac tcctggcaca      120

tccgaaagcg ctacacccga gagtggaccc ggcacctcta ccgagcccag tgagggctcc      180

gcccctggaa caagcaccga gcccagcgaa ggcagcgccc cagggacctc cacagagccc      240

agtgaaggca gtgctcctgg caccagcacc gaaccaagcg agggctctgc acccgggacc      300

tccaccgagc caagcgaagg ctctgcccct ggcacttcca ccgagcccag cgaaggcagc      360

gcccctggga gccccgctgg ctctcccacc agcactgagg agggcacatc taccgaacca      420

agtgaaggct ctgcaccagg tgcctcgagc                                       450


<210>  36
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-4A

<400>  36

Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala 
1               5                   10                  15      


Thr Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
            20                  25                  30          


Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly 
        35                  40                  45              


Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Thr Ser Thr Glu 
    50                  55                  60                  


Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
65                  70                  75                  80  


Ser Gly Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly 
                85                  90                  95      


Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly Ser Pro Ala Gly 
            100                 105                 110         


Ser Pro Thr Ser Thr Glu Glu Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        115                 120                 125             


Ser Gly Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
    130                 135                 140                 


<210>  37
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-4A

<400>  37
ggcgcgccaa cgtccgaaag tgctacccct gagtcaggcc ctggtagtga gcctgccaca       60

agcggaagcg aaactccggg gacctcagag tctgccactc ccgaatcggg gccaggctct      120

gaaccggcca cttcagggag cgaaacacca ggaacatcgg agagcgctac cccggagagc      180

gggccaggaa ctagtactga gcctagcgag ggaagtgcac ctggtacaag cgagtccgcc      240

acacccgagt ctggccctgg ctctccagcg ggctcaccca cgagcactga agagggctct      300

cccgctggca gcccaacgtc gacagaagaa ggatcaccag caggctcccc cacatcaaca      360

gaggagggta catcagaatc tgctactccc gagagtggac ccggtacctc cactgagccc      420

agcgagggga gtgcaccagg tgcctcgagc                                       450


<210>  38
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-5A

<400>  38

Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Ser Glu Pro Ala 
1               5                   10                  15      


Thr Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
            20                  25                  30          


Ser Gly Pro Gly Ser Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly 
        35                  40                  45              


Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly Thr Ser Thr Glu 
    50                  55                  60                  


Pro Ser Glu Gly Ser Ala Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser 
65                  70                  75                  80  


Thr Glu Glu Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly 
                85                  90                  95      


Ser Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser 
            100                 105                 110         


Ala Thr Pro Glu Ser Gly Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser 
        115                 120                 125             


Thr Glu Glu Gly Ser Pro Ala Gly Ser Pro Thr Ser Thr Glu Glu Gly 
    130                 135                 140                 


<210>  39
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-5A

<400>  39
ggcgcgccaa catcagagag cgccacccct gaaagtggtc ccgggagcga gccagccaca       60

tctgggtcgg aaacgccagg cacaagtgag tctgcaactc ccgagtccgg acctggctcc      120

gagcctgcca ctagcggctc cgagactccg ggaacttccg agagcgctac accagaaagc      180

ggacccggaa ccagtaccga acctagcgag ggctctgctc cgggcagccc agccggctct      240

cctacatcca cggaggaggg cacttccgaa tccgccaccc cggagtcagg gccaggatct      300

gaacccgcta cctcaggcag tgagacgcca ggaacgagcg agtccgctac accggagagt      360

gggccaggga gccctgctgg atctcctacg tccactgagg aagggtcacc agcgggctcg      420

cccaccagca ctgaagaagg tgcctcgagc                                       450


<210>  40
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-6B

<400>  40

Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Thr Ser Glu Ser 
1               5                   10                  15      


Ala Thr Pro Glu Ser Gly Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
            20                  25                  30          


Ser Gly Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Pro Gly 
        35                  40                  45              


Ser Glu Pro Ala Thr Ser Gly Ser Glu Thr Pro Gly Ser Glu Pro Ala 
    50                  55                  60                  


Thr Ser Gly Ser Glu Thr Pro Gly Ser Pro Ala Gly Ser Pro Thr Ser 
65                  70                  75                  80  


Thr Glu Glu Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
                85                  90                  95      


Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly Ser Glu Pro Ala 
            100                 105                 110         


Thr Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
        115                 120                 125             


Ser Gly Pro Gly Thr Ser Thr Glu Pro Ser Glu Gly Ser Ala Pro Gly 
    130                 135                 140                 


<210>  41
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AE144-6B

<400>  41
ggcgcgccaa catctaccga gccttccgaa ggctctgccc ctgggacctc agaatctgca       60

acccctgaaa gcggccctgg aacctccgaa agtgccactc ccgagagcgg cccagggaca      120

agcgagtcag caacccctga gtctggaccc ggcagcgagc ctgcaacctc tggctcagag      180

actcccggct cagaacccgc tacctcaggc tccgagacac ccggctctcc tgctgggagt      240

cccacttcca ccgaggaagg aacatccact gagcctagtg agggctctgc ccctggaacc      300

agcacagagc caagtgaggg cagtgcacca ggatccgagc cagcaaccag cgggtccgag      360

actcccggga cctctgagtc tgccacccca gagagcggac ccggcacttc aaccgagccc      420

tccgaaggat cagcaccagg tgcctcgagc                                       450


<210>  42
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-1

<400>  42

Pro Gly Ser Ser Pro Ser Ala Ser Thr Gly Thr Gly Pro Gly Ser Ser 
1               5                   10                  15      


Pro Ser Ala Ser Thr Gly Thr Gly Pro Gly Thr Pro Gly Ser Gly Thr 
            20                  25                  30          


Ala Ser Ser Ser Pro Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser 
        35                  40                  45              


Pro Gly Ser Ser Pro Ser Ala Ser Thr Gly Thr Gly Pro Gly Ala Ser 
    50                  55                  60                  


Pro Gly Thr Ser Ser Thr Gly Ser Pro Gly Thr Pro Gly Ser Gly Thr 
65                  70                  75                  80  


Ala Ser Ser Ser Pro Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser 
                85                  90                  95      


Pro Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro Gly Ala Ser 
            100                 105                 110         


Pro Gly Thr Ser Ser Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser 
        115                 120                 125             


Ser Thr Gly Ser Pro Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser 
    130                 135                 140                 


<210>  43
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-1

<400>  43
ggcgcgccac ccgggtcgtc cccgtcggcg tccaccggaa cagggccagg gtcatccccg       60

tcagcgtcga ctgggacggg acccgggaca cccggttcgg ggactgcatc ctcctcgcct      120

ggttcgtcca ccccgtcagg agccacgggt tcgccgggaa gcagcccaag cgcatccact      180

ggtacagggc ctggggcttc accgggtact tcatccacgg ggtcaccggg aacgcccgga      240

tcggggacgg cttcctcatc accaggatcg tcaacaccct cgggcgcaac gggcagcccc      300

ggaacccctg gttcgggtac ggcgtcgtcg agccccggtg cgagcccggg aacaagctcg      360

acaggatcgc ctggggcgtc acccggcacg tcgagcacag gcagccccgg aacccctgga      420

tcgggaaccg cgtcgtcaag cgcctcgagc                                       450


<210>  44
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-A

<400>  44

Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro Gly Ser Ser Pro 
1               5                   10                  15      


Ser Ala Ser Thr Gly Thr Gly Pro Gly Ser Ser Pro Ser Ala Ser Thr 
            20                  25                  30          


Gly Thr Gly Pro Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro 
        35                  40                  45              


Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro Gly Ser Ser Pro 
    50                  55                  60                  


Ser Ala Ser Thr Gly Thr Gly Pro Gly Ala Ser Pro Gly Thr Ser Ser 
65                  70                  75                  80  


Thr Gly Ser Pro Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro 
                85                  90                  95      


Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro Gly Thr Pro Gly 
            100                 105                 110         


Ser Gly Thr Ala Ser Ser Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser 
        115                 120                 125             


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
    130                 135                 140                 


<210>  45
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-A

<400>  45
ggcgcgccag gtgcctcgcc gggaacatca tcaactggtt cacccgggtc atccccctcg       60

gcctcaaccg ggacgggtcc cggctcatcc cccagcgcca gcactggaac aggtcctggc      120

actcctggtt ccggtacggc atcgtcatcc ccgggaagct caacaccgtc cggagcgaca      180

ggatcacctg gctcgtcacc ttcggcgtca actggaacgg ggccaggggc ctcacccgga      240

acgtcctcga ctgggtcgcc tggtacgccg ggatcaggaa cggcctcatc ctcgcctggg      300

tcctcaacgc cctcgggtgc gactggttcg ccgggaactc ctggctcggg gacggcctcg      360

tcgtcgcctg gggcatcacc ggggacgagc tccacggggt cccctggagc gtcaccgggg      420

acctcctcga caggtagccc ggcctcgagc                                       450


<210>  46
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-B

<400>  46

Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro Gly Ser Ser Thr 
1               5                   10                  15      


Pro Ser Gly Ala Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser 
            20                  25                  30          


Thr Gly Ser Pro Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro 
        35                  40                  45              


Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro Gly Ser Ser Pro 
    50                  55                  60                  


Ser Ala Ser Thr Gly Thr Gly Pro Gly Ser Ser Pro Ser Ala Ser Thr 
65                  70                  75                  80  


Gly Thr Gly Pro Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro 
                85                  90                  95      


Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro Gly Ala Ser Pro 
            100                 105                 110         


Gly Thr Ser Ser Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser 
        115                 120                 125             


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
    130                 135                 140                 


<210>  47
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-B

<400>  47
ggcgcgccag gtacaccggg cagcggcacg gcttcgtcgt cacccggctc gtccacaccg       60

tcgggagcta cgggaagccc aggagcgtca ccgggaacgt cgtcaacggg gtcaccgggt      120

acgccaggta gcggcacggc cagcagctcg ccaggttcat cgaccccgtc gggagcgact      180

gggtcgcccg gatcaagccc gtcagcttcc actggaacag gacccgggtc gtcgccgtca      240

gcctcaacgg ggacaggacc tggttcatcg acgccgtcag gggcgacagg ctcgcccgga      300

tcgtcaacac cctcgggggc aacggggagc cctggtgcgt cgcctggaac ctcatccacc      360

ggaagcccgg gggcctcgcc gggtacgagc tccacgggat cgcccggagc gtcccccgga      420

acttcaagca cagggagccc tgcctcgagc                                       450


<210>  48
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-C

<400>  48

Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro Gly Ala Ser Pro 
1               5                   10                  15      


Gly Thr Ser Ser Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser 
            20                  25                  30          


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
        35                  40                  45              


Gly Ser Ser Pro Ser Ala Ser Thr Gly Thr Gly Pro Gly Thr Pro Gly 
    50                  55                  60                  


Ser Gly Thr Ala Ser Ser Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser 
65                  70                  75                  80  


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
                85                  90                  95      


Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro Gly Ser Ser Thr 
            100                 105                 110         


Pro Ser Gly Ala Thr Gly Ser Pro Gly Ser Ser Thr Pro Ser Gly Ala 
        115                 120                 125             


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
    130                 135                 140                 


<210>  49
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-C

<400>  49
ggcgcgccag gtacacccgg atcgggtaca gcgtcatcga gccccggtgc gtcacctggt       60

acgtcgagca cggggtcgcc aggggcgtcc cctgggacgt cctcaacagg ctcgcccggt      120

gcgtcacccg gcacgtcgtc cacgggttca cctggtagct ccccttccgc gtccactggc      180

accgggcctg gaactccggg gagcggcaca gcgagctcgt cgccgggagc atcgcctggg      240

acatcgagca ccgggtcgcc aggagcatcg cccggaacat ccagcacagg aagccccggc      300

gcgtcgcccg ggacatcaag cacaggttcc ccgggatcga gcacgccgtc cggagccact      360

ggatcaccag ggagctcgac accttccggc gcaacgggat cgcccggagc cagcccgggt      420

acgtcaagca ctggctcccc tgcctcgagc                                       450


<210>  50
<211>  144
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-F

<400>  50

Gly Ser Ser Pro Ser Ala Ser Thr Gly Thr Gly Pro Gly Ser Ser Pro 
1               5                   10                  15      


Ser Ala Ser Thr Gly Thr Gly Pro Gly Ala Ser Pro Gly Thr Ser Ser 
            20                  25                  30          


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
        35                  40                  45              


Gly Ser Ser Thr Pro Ser Gly Ala Thr Gly Ser Pro Gly Ser Ser Pro 
    50                  55                  60                  


Ser Ala Ser Thr Gly Thr Gly Pro Gly Ala Ser Pro Gly Thr Ser Ser 
65                  70                  75                  80  


Thr Gly Ser Pro Gly Ser Ser Pro Ser Ala Ser Thr Gly Thr Gly Pro 
                85                  90                  95      


Gly Thr Pro Gly Ser Gly Thr Ala Ser Ser Ser Pro Gly Ser Ser Thr 
            100                 105                 110         


Pro Ser Gly Ala Thr Gly Ser Pro Gly Ser Ser Thr Pro Ser Gly Ala 
        115                 120                 125             


Thr Gly Ser Pro Gly Ala Ser Pro Gly Thr Ser Ser Thr Gly Ser Pro 
    130                 135                 140                 


<210>  51
<211>  450
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XTEN AG144-F

<400>  51
ggcgcgccag gctccagccc ctccgcgagc acgggaaccg gaccaggttc gtcaccctca       60

gcatcaacgg ggacgggacc gggggcgtca ccaggaacgt cctccaccgg ctcgccgggt      120

gcatcacccg gaacgtcatc gaccggatcg ccagggagct cgacgccatc aggcgcaaca      180

ggatcacctg gctcaagccc tagcgcgtca accggcacgg gtccgggtgc ctcccctggc      240

acgtccagca ccggatcacc cggatcgagc ccatccgcct caaccggaac cggacccggt      300

acaccagggt cgggaacagc ctcctcgtca ccaggctcct caaccccctc gggagccacg      360

ggttcgcccg gttcgtcaac gccttccgga gcaactggta gccccggagc atcgccagga      420

acttcgagca cggggtcgcc cgcctcgagc                                       450


<210>  52
<211>  461
<212>  PRT
<213>  Homo sapiens

<400>  52

Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 
1               5                   10                  15      


Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys Thr Val Phe Leu 
            20                  25                  30          


Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro Lys Arg Tyr Asn 
        35                  40                  45              


Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg Glu Cys 
    50                  55                  60                  


Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe Glu Asn 
65                  70                  75                  80  


Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly Asp Gln 
                85                  90                  95      


Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp Asp Ile 
            100                 105                 110         


Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys Asn Cys 
        115                 120                 125             


Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu Gln Phe 
    130                 135                 140                 


Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr Glu Gly 
145                 150                 155                 160 


Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val Pro Phe 
                165                 170                 175     


Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr Arg Ala 
            180                 185                 190         


Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu Ala Glu 
        195                 200                 205             


Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn Asp Phe 
    210                 215                 220                 


Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe Pro Trp 
225                 230                 235                 240 


Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly Ser Ile 
                245                 250                 255     


Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu Thr Gly 
            260                 265                 270         


Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu Thr Glu 
        275                 280                 285             


His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His His Asn 
    290                 295                 300                 


Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu Leu Glu 
305                 310                 315                 320 


Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile Cys Ile 
                325                 330                 335     


Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser Gly Tyr 
            340                 345                 350         


Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala Leu Val 
        355                 360                 365             


Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys Leu Arg 
    370                 375                 380                 


Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly Phe His 
385                 390                 395                 400 


Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro His Val 
                405                 410                 415     


Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser Trp Gly 
            420                 425                 430         


Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys Val Ser 
        435                 440                 445             


Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 
    450                 455                 460     


<210>  53
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of factor IX variant, R338L

<400>  53
atgcagagag tcaacatgat tatggctgag tcacctgggc tgattactat ttgcctgctg       60

ggctacctgc tgtccgccga gtgtaccgtg ttcctggacc atgagaacgc aaataagatc      120

ctgaacaggc ccaaaagata caatagtggg aagctggagg aatttgtgca gggcaacctg      180

gagagagaat gcatggagga aaagtgtagc ttcgaggaag cccgcgaggt gtttgaaaat      240

acagagcgaa ccacagagtt ctggaagcag tatgtggacg gcgatcagtg cgagagcaac      300

ccctgtctga atggcggaag ttgcaaagac gatatcaact catacgaatg ctggtgtcct      360

ttcgggtttg aaggcaaaaa ttgcgagctg gacgtgacat gtaacattaa gaatggacgg      420

tgcgagcagt tttgtaaaaa ctctgccgat aataaggtgg tgtgcagctg tactgaagga      480

tatcgcctgg ctgagaacca gaagtcctgc gaaccagcag tgcccttccc ttgtgggagg      540

gtgagcgtct cccagacttc aaaactgacc agagcagaga cagtgtttcc cgacgtggat      600

tacgtcaaca gcactgaggc cgaaaccatc ctggacaaca ttactcagtc tacccagagt      660

ttcaatgact ttactcgggt ggtcgggggc gaggatgcta aaccaggcca gttcccctgg      720

caggtggtcc tgaacggaaa ggtggatgca ttttgcggag ggtctatcgt gaatgagaaa      780

tggattgtca ccgccgctca ctgcgtggaa accggagtca agatcacagt ggtcgctggg      840

gagcacaaca ttgaggaaac agaacatact gagcagaagc ggaatgtgat ccgcatcatt      900

cctcaccata actacaatgc agccatcaac aaatacaatc atgacattgc cctgctggaa      960

ctggatgagc ctctggtgct gaacagctac gtcactccaa tctgcattgc tgacaaagag     1020

tataccaata tcttcctgaa gtttggatca gggtacgtga gcggctgggg aagagtcttc     1080

cacaagggca ggagcgccct ggtgctccag tatctgcgag tgcctctggt cgatcgagct     1140

acctgtctgc tgtctaccaa gtttacaatc tacaacaaca tgttctgcgc tgggtttcac     1200

gagggaggac gagactcctg tcagggcgat tctgggggcc cacatgtgac agaggtcgaa     1260

ggcaccagct tcctgactgg catcatttcc tggggagagg aatgtgcaat gaagggaaaa     1320

tacgggatct acaccaaagt gagccgctat gtgaactgga tcaaggaaaa aaccaaactg     1380

acctaa                                                                1386


<210>  54
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIX-R338L-co2

<400>  54
atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc      120

ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg      180

gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac      240

accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc      480

tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg      540

gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc      660

ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcccggcca gttcccctgg      720

caggtcgtgc tgaatggcaa agtcgatgcc ttctgcgggg gcagcatcgt caacgagaag      780

tggattgtga ctgccgccca ttgcgtggaa accggggtga agatcactgt ggtggctggg      840

gagcacaaca tcgaggaaac cgagcacacc gagcagaaga ggaacgtgat caggattatc      900

ccccatcaca actacaatgc cgccatcaat aagtacaacc atgatattgc cctgctggag      960

ctggatgaac ccctggtcct gaacagctat gtgactccca tctgcattgc cgacaaggag     1020

tataccaaca tcttcctgaa atttggcagc ggctatgtct ctggctgggg cagggtgttc     1080

cataagggga ggagcgccct ggtcctgcag tacctgagag tgcccctggt ggacagggcc     1140

acctgcctgc tgagcaccaa gttcaccatc tacaacaata tgttttgcgc tggcttccat     1200

gaggggggca gggacagctg ccagggggac agcgggggcc cccatgtgac tgaggtggag     1260

ggcaccagct tcctgaccgg catcatcagc tggggggagg agtgcgccat gaaggggaag     1320

tatggcatct acaccaaagt ctccagatac gtcaactgga tcaaggagaa gaccaagctg     1380

acctaa                                                                1386


<210>  55
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIX-R338L-co3

<400>  55
atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc      120

ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg      180

gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac      240

accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc      480

tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg      540

gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc      660

ttcaacgact tcaccagggt ggtgggcggc gaggacgcca agcccggcca gtttccctgg      720

caggtggtgc tgaacggcaa ggtggacgcc ttctgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga ccgctgccca ttgcgtggaa accggcgtga agatcaccgt ggtggccggc      840

gagcacaaca tcgaagagac cgagcacacc gaacagaaaa ggaacgtgat caggatcatc      900

cctcaccata actacaatgc cgccattaac aagtacaatc acgacatcgc tctgctggaa      960

ctggatgaac ccctggtgct gaacagctac gtgaccccta tctgcatcgc cgacaaggag     1020

tatactaaca tctttctgaa gtttggcagc ggctatgtga gcggctgggg cagggtgttc     1080

cacaaaggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggatagggct     1140

acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgtgc cggcttccac     1200

gaaggcggca gggactcttg ccagggcgac agcggcggcc cccatgtgac cgaggtggaa     1260

ggcactagct ttctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag     1320

tacggcatct acactaaggt gagcaggtac gtgaactgga tcaaagaaaa gaccaagctg     1380

acctaa                                                                1386


<210>  56
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIX-R338L-co4

<400>  56
atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc      120

ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg      180

gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac      240

accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac      300

ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc      360

ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg      420

tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc      480

tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg      540

gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac      600

tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc      660

ttcaacgact tcaccagggt ggtgggcggc gaggacgcca agcccggcca gttcccctgg      720

caggtggtgc tgaacggcaa ggtggacgcc ttctgcggcg gcagcatcgt gaacgagaag      780

tggatcgtga ccgccgccca ctgcgtggag accggcgtga agatcaccgt ggtggccggc      840

gagcacaaca tcgaggagac cgagcacacc gagcagaaga ggaacgtgat caggatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag      960

ctggacgagc ccctggtgct gaacagctac gtgaccccca tctgcatcgc cgacaaggag     1020

tacaccaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc     1080

cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc     1140

acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac     1200

gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag     1260

ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag     1320

tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg     1380

acctaa                                                                1386


<210>  57
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIX-R338L-co5

<400>  57
atgcagaggg tcaacatgat catggctgag tctcctggcc tgatcaccat ctgcctgctg       60

ggctatctgc tgtccgctga gtgcactgtc ttcctggacc acgagaacgc caacaagatc      120

ctgaacaggc ccaagaggta taactctggc aagctggagg agtttgtgca ggggaacctg      180

gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gtttgagaac      240

actgagagga ccaccgagtt ctggaagcag tatgtggacg gggaccagtg cgagtctaac      300

ccttgcctga acgggggcag ctgcaaggat gacatcaaca gctatgagtg ctggtgccct      360

ttcggcttcg agggcaagaa ctgcgagctg gatgtgacct gcaacatcaa gaacggcagg      420

tgcgagcagt tctgcaagaa ctctgccgac aacaaggtgg tgtgcagctg cactgagggc      480

tataggctgg ctgagaacca gaagagctgt gagcctgctg tgcccttccc ctgcggcaga      540

gtgtctgtga gccagaccag caagctgacc agagctgaga ctgtcttccc cgacgtggac      600

tatgtgaaca gcaccgaggc tgagaccatc ctggacaaca tcacccagtc tacccagtct      660

ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcctggcca gttcccctgg      720

caggtcgtgc tgaacggcaa agtggacgcc ttctgcgggg gcagcatcgt caacgagaag      780

tggatcgtga ctgctgctca ctgcgtggaa accggggtga agatcaccgt ggtggccggc      840

gagcacaaca tcgaggagac cgagcacacc gagcagaaga ggaacgtgat caggatcatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag      960

ctggacgagc ccctggtgct gaacagctac gtgaccccca tctgcatcgc cgacaaggag     1020

tacaccaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc     1080

cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc     1140

acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac     1200

gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag     1260

ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag     1320

tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg     1380

acctaa                                                                1386


<210>  58
<211>  1386
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FIX-R338L-co6

<400>  58
atgcagaggg tcaacatgat catggctgag tctcctggcc tgatcactat ctgcctgctg       60

ggctacctgc tgagcgccga gtgcactgtc ttcctggacc acgagaacgc caacaagatc      120

ctgaacaggc ccaagagata caactctggc aagctggagg agtttgtgca ggggaacctg      180

gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gtttgagaac      240

actgagagga ccactgagtt ctggaagcag tatgtggacg gggaccagtg cgagtctaac      300

ccttgcctga acgggggcag ctgcaaggat gacatcaaca gctacgagtg ctggtgccct      360

ttcggcttcg agggcaagaa ctgcgagctg gatgtgactt gcaacatcaa gaacggcagg      420

tgcgagcagt tctgcaagaa ctctgccgac aacaaagtcg tgtgcagctg cactgagggc      480

tacagactgg ctgagaacca gaagagctgt gagcctgctg tgcccttccc ctgcggcaga      540

gtgtctgtga gccagaccag caagctgacc agagccgaaa ccgtgttccc cgacgtggac      600

tatgtgaaca gcactgaggc tgagaccatc ctggacaaca tcactcagtc tacccagtct      660

ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcctggcca gttcccctgg      720

caggtcgtgc tgaacggcaa ggtggacgcc ttctgcgggg gcagcatcgt caacgagaag      780

tggatcgtga ctgccgccca ctgcgtggag actggggtga agatcactgt ggtggctggg      840

gagcacaaca tcgaggaaac cgagcacact gagcagaaga ggaacgtgat caggattatc      900

ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag      960

ctggatgaac ccctggtgct gaacagctac gtgaccccta tctgcatcgc cgacaaggag     1020

tacactaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc     1080

cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc     1140

acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac     1200

gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag     1260

ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag     1320

tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg     1380

acctaa                                                                1386