                         SEQUENCE LISTING

<110>  TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED
       SHAINSKY-ROITMAN, Janna
       Mor GODLFEDER
	Nitzan KRINSKY
	SCHROEDER, Avraham
       SHOHAM, Yuval
 
<120>  METHODS AND KITS FOR CELL-FREE PROTEIN SYNTHESIS

<130>  62979

<150>  US 62/021,748
<151>  2014-07-08

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  945
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding cloning vector pBS 35S attR-hRluc, 
       complete sequence

<400>  1
atggcttcga aggtgtacga ccccgagcag aggaagagga tgatcaccgg cccccagtgg       60

tgggccaggt gcaagcagat gaacgtgctg gacagcttca tcaactacta cgacagcgag      120

aagcacgccg agaacgccgt gatcttcctg cacggcaacg ccgctagcag ctacctgtgg      180

aggcacgtgg tgccccacat cgagcccgtg gccaggtgca tcatccccga tctgatcggc      240

atgggcaaga gcggcaagag cggcaacggc agctacaggc tgctggacca ctacaagtac      300

ctgaccgcct ggttcgagct cctgaacctg cccaagaaga tcatcttcgt gggccacgac      360

tggggcgcct gcctggcctt ccactacagc tacgagcacc aggacaagat caaggccatc      420

gtgcacgccg agagcgtggt ggacgtgatc gagagctggg acgagtggcc agacatcgag      480

gaggacatcg ccctgatcaa gagcgaggag ggcgagaaga tggtgctgga gaacaacttc      540

ttcgtggaga ccatgctgcc cagcaagatc atgagaaagc tggagcccga ggagttcgcc      600

gcctacctgg agcccttcaa ggagaagggc gaggtgagaa gacccaccct gagctggccc      660

agagagatcc ccctggtgaa gggcggcaag cccgacgtgg tgcagatcgt gagaaactac      720

aacgcctacc tgagagccag cgacgacctg cccaagatgt tcatcgagag cgaccccggc      780

ttcttcagca acgccatcgt ggagggcgcc aagaagttcc ccaacaccga gttcgtgaag      840

gtgaagggcc tgcacttcag ccaggaggac gcccccgacg agatgggcaa gtacatcaag      900

agcttcgtgg agagagtgct gaagaacgag cagagatcta tctag                      945


<210>  2
<211>  894
<212>  DNA
<213>  Bacillus megaterium

<400>  2
atgagtaaca agtatagagt tagaaaaaac gtattacatc ttaccgacac ggaaaaaaga       60

gattttgttc gtaccgtgct aatactaaag gaaaaaggga tatatgaccg ctatatagcc      120

tggcatggtg cagcaggtaa atttcatact cctccgggca gcgatcgaaa tgcagcacat      180

atgagttctg cttttttacc gtggcatcgt gaataccttt tacgattcga acgtgacctt      240

cagtcaatca atccagaagt aacccttcct tattgggaat gggaaacgga cgcacagatg      300

caggatccct cacaatcaca aatttggagt gcagatttta tgggaggaaa cggaaatccc      360

ataaaagatt ttatcgtcga taccgggcca tttgcagctg ggcgctggac gacgatcgat      420

gaacaaggaa atccttccgg agggctaaaa cgtaattttg gagcaacgaa agaggcacct      480

acactcccta ctcgagatga tgtcctcaat gctttaaaaa taactcagta tgatacgccg      540

ccttgggata tgaccagcca aaacagcttt cgtaatcagc ttgaaggatt tattaacggg      600

ccacagcttc acaatcgcgt acaccgttgg gttggcggac agatgggcgt tgtgcctact      660

gctccgaatg atcctgtctt ctttttacac cacgcaaatg tggatcgtat ttgggctgta      720

tggcaaatta ttcatcgtaa tcaaaactat cagccgatga aaaacgggcc atttggtcaa      780

aactttagag atccgatgta cccttggaat acaacccctg aagacgttat gaaccatcga      840

aagcttgggt acgtatacga tatagaatta agaaaatcaa aacgttcctc ataa            894


<210>  3
<211>  723
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide squence encoding Super folder GFP

<400>  3
catatgagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat       60

ggtgatgtta atgggcacaa attttctgtc cgtggagagg gtgaaggtga tgctacaaac      120

ggaaaactca cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca      180

cttgtcacta ctctgaccta tggtgttcaa tgcttttccc gttatccgga tcacatgaaa      240

cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct      300

ttcaaagatg acgggaccta caagacgcgt gctgaagtca agtttgaagg tgataccctt      360

gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac      420

aaactcgagt acaactttaa ctcacacaat gtatacatca cggcagacaa acaaaagaat      480

ggaatcaaag ctaacttcaa aattcgccac aacgttgaag atggttccgt tcaactagca      540

gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat      600

tacctgtcga cacaatctgt cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc      660

cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaagga      720

tcc                                                                    723


<210>  4
<211>  2910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding  p50 (NF-kB subunit)

<400>  4
atggcagaag atgatccata tttgggaagg cctgaacaaa tgtttcattt ggatccttct       60

ttgactcata caatatttaa tccagaagta tttcaaccac agatggcact gccaacagat      120

ggcccatacc ttcaaatctt agagcaacct aaacagagag gatttcgttt ccgttatgta      180

tgtgaaggcc catcccatgg tggactacct ggtgcctcta gtgaaaagaa caagaagtct      240

taccctcagg tcaaaatctg caactatgtg ggaccagcaa aggttattgt tcagttggtc      300

acaaatggaa aaaatatcca cctgcatgcc cacagcctgg tgggaaaaca ctgtgaggat      360

gggatctgca ctgtaactgc tggacccaag gacatggtgg tcggcttcgc aaacctgggt      420

atacttcatg tgacaaagaa aaaagtattt gaaacactgg aagcacgaat gacagaggcg      480

tgtataaggg gctataatcc tggactcttg gtgcaccctg accttgccta tttgcaagca      540

gaaggtggag gggaccggca gctgggagat cgggaaaaag agctaatccg ccaagcagct      600

ctgcagcaga ccaaggagat ggacctcagc gtggtgcggc tcatgtttac agcttttctt      660

ccggatagca ctggcagctt cacaaggcgc ctggaacccg tggtatcaga cgccatctat      720

gacagtagtg aagcccccaa tgcatccaac ttgaaaattg taagaatgga caggacagct      780

ggatgtgtga ctggagggga ggaaatttat cttctttgtg acaaagttca gaaagatgac      840

atccagattc gattttatga agaggaagaa aatggtggag tctgggaagg atttggagat      900

ttttccccca cagatgttca tagacaattt gccattgtct tcaaaactcc aaagtataaa      960

gatattaata ttacaaaacc agcctctgtg tttgtccagc ttcggaggaa atctgacttg     1020

gaaactagtg aaccaaaacc tttcctctac tatcctgaaa tcaaagataa agaagaagtg     1080

cagaggaaac gtcagaagct catgcccaat ttttcggata gtttcggcgg tggtagtggt     1140

gccggagctg gaggcggagg catgtttggt agtggcggtg gaggaggggg cactggaagt     1200

acaggtccag ggtatagctt cccacactat ggatttccta cttatggtgg gattactttc     1260

catcctggaa ctactaaatc taatgctggg atgaagcatg gaaccatgga cactgaatct     1320

aaaaaggacc ctgaaggttg tgacaaaagt gatgacaaaa acactgtaaa cctctttggg     1380

aaagttattg aaaccacaga gcaagatcag gagcccagcg aggccaccgt tgggaatggt     1440

gaggtcactc taacgtatgc aacaggaaca aaagaagaga gtgctggagt tcaggataac     1500

ctctttctag agaaggctat gcagcttgca aagaggcatg ccaatgccct tttcgactac     1560

gcggtgacag gagacgtgaa gatgctgctg gccgtccagc gccatctcac tgctgtgcag     1620

gatgagaatg gggacagtgt cttacactta gcaagcagcc accttcattc tcaacttgtg     1680

agggatctac tagaagtcac atctggtttg atttctgatg acattatcaa catgagaaat     1740

gatctgtacc agacgccctt gcacttggca gtgatcacta agcaggaaga tgtggtggag     1800

gatttgctga gggctggggc cgacctgagc cttctggacc gcttgggtaa ctctgttttg     1860

cacctagctg ccaaagaagg acatgataaa gttctcagta tcttactcaa gcacaaaaag     1920

gcagcactac ttcttgacca ccccaacggg gacggtctga atgccattca tctagccatg     1980

atgagcaata gcctgccatg tttgctgctg ctggtggccg ctggggctga cgtcaatgct     2040

caggagcaga agtccgggcg cacagcactg cacctggctg tggagcacga caacatctca     2100

ttggcaggct gcctgctcct ggagggtgat gcccatgtgg acagtactac ctacgatgga     2160

accacacccc tgcatatagt agctgggaga gggtccacca ggctggcagc tcttctcaaa     2220

gcagcaggag cagatcccct ggtggagaac tttgagcctc tctatgacct ggatgactct     2280

tgggaaaatg caggagagga tgaaggagtt gtgcctggaa ccacgcctct agatatggcc     2340

accagctggc aggtatttga catattaaat gggaaaccat atgagccaga gtttacatct     2400

gatgatttac tagcacaagg agacatgaaa cagctggctg aagatgtgaa gctgcagctg     2460

tataagttac tagaaattcc tgatccagac aaaaactggg ctactctggc gcagaaatta     2520

ggtctgggga tacttaataa tgccttccgg ctgagtcctg ctccttccaa aacacttatg     2580

gacaactatg aggtctctgg gggtacgatc agagagctgg tggaggccct gagacaaatg     2640

ggctacaccg aagcaattga agtgatccag gcagcctcca gcccagtgaa gaccacctct     2700

caggcccact cgctgcctct ctcgcctgcc tccacaaggc agcaaataga cgagctccga     2760

gacagtgaca gtgtctgcga cacgggcgtg gagacatcct tccgcaaact cagctttacc     2820

gagtctctga ccagtggtgc ctcactgcta actctcaaca aaatgcccca tgattatggg     2880

caggaaggac ctctagaagg caaaatttag                                      2910


<210>  5
<211>  2910
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of Pet3 Plasmid encoding for Pseudomonas 
       exotoxin A (PE)

<400>  5
atggcagaag atgatccata tttgggaagg cctgaacaaa tgtttcattt ggatccttct       60

ttgactcata caatatttaa tccagaagta tttcaaccac agatggcact gccaacagat      120

ggcccatacc ttcaaatctt agagcaacct aaacagagag gatttcgttt ccgttatgta      180

tgtgaaggcc catcccatgg tggactacct ggtgcctcta gtgaaaagaa caagaagtct      240

taccctcagg tcaaaatctg caactatgtg ggaccagcaa aggttattgt tcagttggtc      300

acaaatggaa aaaatatcca cctgcatgcc cacagcctgg tgggaaaaca ctgtgaggat      360

gggatctgca ctgtaactgc tggacccaag gacatggtgg tcggcttcgc aaacctgggt      420

atacttcatg tgacaaagaa aaaagtattt gaaacactgg aagcacgaat gacagaggcg      480

tgtataaggg gctataatcc tggactcttg gtgcaccctg accttgccta tttgcaagca      540

gaaggtggag gggaccggca gctgggagat cgggaaaaag agctaatccg ccaagcagct      600

ctgcagcaga ccaaggagat ggacctcagc gtggtgcggc tcatgtttac agcttttctt      660

ccggatagca ctggcagctt cacaaggcgc ctggaacccg tggtatcaga cgccatctat      720

gacagtagtg aagcccccaa tgcatccaac ttgaaaattg taagaatgga caggacagct      780

ggatgtgtga ctggagggga ggaaatttat cttctttgtg acaaagttca gaaagatgac      840

atccagattc gattttatga agaggaagaa aatggtggag tctgggaagg atttggagat      900

ttttccccca cagatgttca tagacaattt gccattgtct tcaaaactcc aaagtataaa      960

gatattaata ttacaaaacc agcctctgtg tttgtccagc ttcggaggaa atctgacttg     1020

gaaactagtg aaccaaaacc tttcctctac tatcctgaaa tcaaagataa agaagaagtg     1080

cagaggaaac gtcagaagct catgcccaat ttttcggata gtttcggcgg tggtagtggt     1140

gccggagctg gaggcggagg catgtttggt agtggcggtg gaggaggggg cactggaagt     1200

acaggtccag ggtatagctt cccacactat ggatttccta cttatggtgg gattactttc     1260

catcctggaa ctactaaatc taatgctggg atgaagcatg gaaccatgga cactgaatct     1320

aaaaaggacc ctgaaggttg tgacaaaagt gatgacaaaa acactgtaaa cctctttggg     1380

aaagttattg aaaccacaga gcaagatcag gagcccagcg aggccaccgt tgggaatggt     1440

gaggtcactc taacgtatgc aacaggaaca aaagaagaga gtgctggagt tcaggataac     1500

ctctttctag agaaggctat gcagcttgca aagaggcatg ccaatgccct tttcgactac     1560

gcggtgacag gagacgtgaa gatgctgctg gccgtccagc gccatctcac tgctgtgcag     1620

gatgagaatg gggacagtgt cttacactta gcaagcagcc accttcattc tcaacttgtg     1680

agggatctac tagaagtcac atctggtttg atttctgatg acattatcaa catgagaaat     1740

gatctgtacc agacgccctt gcacttggca gtgatcacta agcaggaaga tgtggtggag     1800

gatttgctga gggctggggc cgacctgagc cttctggacc gcttgggtaa ctctgttttg     1860

cacctagctg ccaaagaagg acatgataaa gttctcagta tcttactcaa gcacaaaaag     1920

gcagcactac ttcttgacca ccccaacggg gacggtctga atgccattca tctagccatg     1980

atgagcaata gcctgccatg tttgctgctg ctggtggccg ctggggctga cgtcaatgct     2040

caggagcaga agtccgggcg cacagcactg cacctggctg tggagcacga caacatctca     2100

ttggcaggct gcctgctcct ggagggtgat gcccatgtgg acagtactac ctacgatgga     2160

accacacccc tgcatatagt agctgggaga gggtccacca ggctggcagc tcttctcaaa     2220

gcagcaggag cagatcccct ggtggagaac tttgagcctc tctatgacct ggatgactct     2280

tgggaaaatg caggagagga tgaaggagtt gtgcctggaa ccacgcctct agatatggcc     2340

accagctggc aggtatttga catattaaat gggaaaccat atgagccaga gtttacatct     2400

gatgatttac tagcacaagg agacatgaaa cagctggctg aagatgtgaa gctgcagctg     2460

tataagttac tagaaattcc tgatccagac aaaaactggg ctactctggc gcagaaatta     2520

ggtctgggga tacttaataa tgccttccgg ctgagtcctg ctccttccaa aacacttatg     2580

gacaactatg aggtctctgg gggtacgatc agagagctgg tggaggccct gagacaaatg     2640

ggctacaccg aagcaattga agtgatccag gcagcctcca gcccagtgaa gaccacctct     2700

caggcccact cgctgcctct ctcgcctgcc tccacaaggc agcaaataga cgagctccga     2760

gacagtgaca gtgtctgcga cacgggcgtg gagacatcct tccgcaaact cagctttacc     2820

gagtctctga ccagtggtgc ctcactgcta actctcaaca aaatgcccca tgattatggg     2880

caggaaggac ctctagaagg caaaatttag                                      2910


<210>  6
<211>  1900
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding Pseudomonas exotoxin A with 
       histidine tag

<400>  6
ctagaaataa agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag       60

cgccgaagaa gctttcgacc tctggaacga atgcgccaaa gcctgcgtgc tcgacctcaa      120

ggacggcgtg cgttccagcc gcatgagcgt cgacccggcc atcgccgaca ccaacggcca      180

gggcgtgctg cactactcca tggtcctgga gggcggcaac gacgcgctca agctggccat      240

cgacaacgcc ctcagcatca ccagcgacgg cctgaccatc cgcctcgaag gcggcgtcga      300

gccgaacaag ccggtgcgct acagctacac gcgccaggcg cgcggcagtt ggtcgctgaa      360

ctggctggta ccgatcggcc acgagaagcc ctcgaacatc aaggtgttca tccacgaact      420

gaacgccggc aaccagctca gccacatgtc gccgatctac accatcgaga tgggcgacga      480

gttgctggcg aagctggcgc gcgatgccac cttcttcgtc agggcgcacg agagcaacga      540

gatgcagccg acgctcgcca tcagccatgc cggggtcagc gtggtcatgg cccagaccca      600

gccgcgccgg gaaaagcgct ggagcgaatg ggccagcggc aaggtgttgt gcctgctcga      660

cccgctggac ggggtctaca actacctcgc ccagcaacgc tgcaacctcg acgatacctg      720

ggaaggcaag atctaccggg tgctcgccgg caacccggcg aagcatgacc tggacatcaa      780

acccacggtc atcagtcatc gcctgcactt tcccgagggc ggcagcctgg ccgcgctgac      840

cgcgcaccag gcttgccacc tgccgctgga gactttcacc cgtcatcgcc agccgcgcgg      900

ctgggaacaa ctggagcagt gcggctatcc ggtgcagcgg ctggtcgccc tctacctggc      960

ggcgcggctg tcgtggaacc aggtcgacca ggtgatccgc aacgccctgg ccagccccgg     1020

cagcggcggc gacctgggcg aagcgatccg cgagcagccg gagcaggccc gtctggccct     1080

gaccctggcc gccgccgaga gcgagcgctt cgtccggcag ggcaccggca acgacgaggc     1140

cggcgcggcc aacgccgacg tggtgagcct gacctgcccg gtcgccgccg gtgaatgcgc     1200

gggcccggcg gacagcggcg acgccctgct ggagcgcaac tatcccactg gcgcggagtt     1260

cctcggcgac ggcggcgacg tcagcttcag cacccgcggc acgcagaact ggacggtgga     1320

gcggctgctc caggcgcacc gccaactgga ggagcgcggc tatgtgttcg tcggctacca     1380

cggcaccttc ctcgaagcgg cgcaaagcat cgtcttcggc ggggtgcgcg cgcgcagcca     1440

ggacctcgac gcgatctggc gcggtttcta tatcgccggc gatccggcgc tggcctacgg     1500

ctacgcccag gaccaggaac ccgacgcacg cggccggatc cgcaacggtg ccctgctgcg     1560

ggtctatgtg ccgcgctcga gcctgccggg cttctaccgc accagcctga ccctggccgc     1620

gccggaggcg gcgggcgagg tcgaacggct gatcggccat ccgctgccgc tgcgcctgga     1680

cgccatcacc ggccccgagg aggaaggcgg gcgcctggag accattctcg gctggccgct     1740

ggccgagcgc accgtggtga ttccctcggc gatccccacc gacccgcgca acgtcggcgg     1800

cgacctcgac ccgtccagca tcccggacca agaacaggcg atcagcgccc tgccggacta     1860

cgccagccag cccgggcaac cgccgcgcga ggacctgtag                           1900


