                         SEQUENCE LISTING

<110>  Stallergenes S.A.
 
<120>  COMPOSITION OF ALLERGEN EXTRACTS HAVING REDUCED TOXICITY AND 
       METHOD OF PRODUCTION THEREOF

<130>  BET 15P1779

<150>  EP14306156.2
<151>  2014-07-16

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  417
<212>  DNA
<213>  Ambrosia artemisiifolia


<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  CDS
<222>  (1)..(417)

<400>  1
atg gct aac aaa aat ttg ggc atc ttc atc ctc ata gca aca atc gta         48
Met Ala Asn Lys Asn Leu Gly Ile Phe Ile Leu Ile Ala Thr Ile Val           
1               5                   10                  15                

gct cag gct aca tgg gtt cga ggg gca acg cag cat ata gtt gga gac         96
Ala Gln Ala Thr Trp Val Arg Gly Ala Thr Gln His Ile Val Gly Asp           
            20                  25                  30                    

gat aaa ggc tgg gcc ctg ggt gtt gat tac gta gct tgg gcc aat gcg        144
Asp Lys Gly Trp Ala Leu Gly Val Asp Tyr Val Ala Trp Ala Asn Ala           
        35                  40                  45                        

agg caa atc gtc cag gga gat gaa ctt gtg ttc aat tac gat gtg aaa        192
Arg Gln Ile Val Gln Gly Asp Glu Leu Val Phe Asn Tyr Asp Val Lys           
    50                  55                  60                            

caa gac ttt ccc gta ttt tac acg act aaa gat aaa ttt gaa cga tgt        240
Gln Asp Phe Pro Val Phe Tyr Thr Thr Lys Asp Lys Phe Glu Arg Cys           
65                  70                  75                  80            

tgt cct tat gga gca tta atg gaa ttg gct aat act gga cat aat gtg        288
Cys Pro Tyr Gly Ala Leu Met Glu Leu Ala Asn Thr Gly His Asn Val           
                85                  90                  95                

gtc acc tta gga ggc gtg ggc gac tat tat ttt att cct aaa gga gtt        336
Val Thr Leu Gly Gly Val Gly Asp Tyr Tyr Phe Ile Pro Lys Gly Val           
            100                 105                 110                   

gat tgt aaa caa aat atg aag ctt cat gtc aat gtc aag gca agt cct        384
Asp Cys Lys Gln Asn Met Lys Leu His Val Asn Val Lys Ala Ser Pro           
        115                 120                 125                       

gca tta cag gag gga cga aac gcc ata ctg gca                            417
Ala Leu Gln Glu Gly Arg Asn Ala Ile Leu Ala                               
    130                 135                                               


<210>  2
<211>  139
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  2

Met Ala Asn Lys Asn Leu Gly Ile Phe Ile Leu Ile Ala Thr Ile Val 
1               5                   10                  15      


Ala Gln Ala Thr Trp Val Arg Gly Ala Thr Gln His Ile Val Gly Asp 
            20                  25                  30          


Asp Lys Gly Trp Ala Leu Gly Val Asp Tyr Val Ala Trp Ala Asn Ala 
        35                  40                  45              


Arg Gln Ile Val Gln Gly Asp Glu Leu Val Phe Asn Tyr Asp Val Lys 
    50                  55                  60                  


Gln Asp Phe Pro Val Phe Tyr Thr Thr Lys Asp Lys Phe Glu Arg Cys 
65                  70                  75                  80  


Cys Pro Tyr Gly Ala Leu Met Glu Leu Ala Asn Thr Gly His Asn Val 
                85                  90                  95      


Val Thr Leu Gly Gly Val Gly Asp Tyr Tyr Phe Ile Pro Lys Gly Val 
            100                 105                 110         


Asp Cys Lys Gln Asn Met Lys Leu His Val Asn Val Lys Ala Ser Pro 
        115                 120                 125             


Ala Leu Gln Glu Gly Arg Asn Ala Ile Leu Ala 
    130                 135                 


<210>  3
<211>  115
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  3

Ala Thr Gln His Ile Val Gly Asp Asp Lys Gly Trp Ala Leu Gly Val 
1               5                   10                  15      


Asp Tyr Val Ala Trp Ala Asn Ala Arg Gln Ile Val Gln Gly Asp Glu 
            20                  25                  30          


Leu Val Phe Asn Tyr Asp Val Lys Gln Asp Phe Pro Val Phe Tyr Thr 
        35                  40                  45              


Thr Lys Asp Lys Phe Glu Arg Cys Cys Pro Tyr Gly Ala Leu Met Glu 
    50                  55                  60                  


Leu Ala Asn Thr Gly His Asn Val Val Thr Leu Gly Gly Val Gly Asp 
65                  70                  75                  80  


Tyr Tyr Phe Ile Pro Lys Gly Val Asp Cys Lys Gln Asn Met Lys Leu 
                85                  90                  95      


His Val Asn Val Lys Ala Ser Pro Ala Leu Gln Glu Gly Arg Asn Ala 
            100                 105                 110         


Ile Leu Ala 
        115 


<210>  4
<211>  366
<212>  DNA
<213>  Ambrosia artemisiifolia


<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  CDS
<222>  (1)..(366)

<400>  4
atg gct aac aaa agt ttg ggc atc ttc atc ctc ata gca aca cta gta         48
Met Ala Asn Lys Ser Leu Gly Ile Phe Ile Leu Ile Ala Thr Leu Val           
1               5                   10                  15                

gct caa act aca tgg gtt cga gca gtg gac cat ata gtt gga gag gag         96
Ala Gln Thr Thr Trp Val Arg Ala Val Asp His Ile Val Gly Glu Glu           
            20                  25                  30                    

aaa ggt tgg gtc acg ggt gtc gac ttc gaa gct tgg gca aaa gcg agg        144
Lys Gly Trp Val Thr Gly Val Asp Phe Glu Ala Trp Ala Lys Ala Arg           
        35                  40                  45                        

aca ttc tct gta gga gac caa ctt gtg ttc aaa tat gct ccg aat tat        192
Thr Phe Ser Val Gly Asp Gln Leu Val Phe Lys Tyr Ala Pro Asn Tyr           
    50                  55                  60                            

ggt cta ttg ctt acg gat aaa gtg cgt tat gac ata tgt tgt cct cga        240
Gly Leu Leu Leu Thr Asp Lys Val Arg Tyr Asp Ile Cys Cys Pro Arg           
65                  70                  75                  80            

gga cca att ttg gca cta gaa tct act gga gat act acg gtc aaa ata        288
Gly Pro Ile Leu Ala Leu Glu Ser Thr Gly Asp Thr Thr Val Lys Ile           
                85                  90                  95                

caa ggg cca ggc gac tat tac ttt att tcg tac agg cct tat tgt ttt        336
Gln Gly Pro Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys Phe           
            100                 105                 110                   

gag gga tgt aag ctt cac atc acc gtc aag                                366
Glu Gly Cys Lys Leu His Ile Thr Val Lys                                   
        115                 120                                           


<210>  5
<211>  122
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  5

Met Ala Asn Lys Ser Leu Gly Ile Phe Ile Leu Ile Ala Thr Leu Val 
1               5                   10                  15      


Ala Gln Thr Thr Trp Val Arg Ala Val Asp His Ile Val Gly Glu Glu 
            20                  25                  30          


Lys Gly Trp Val Thr Gly Val Asp Phe Glu Ala Trp Ala Lys Ala Arg 
        35                  40                  45              


Thr Phe Ser Val Gly Asp Gln Leu Val Phe Lys Tyr Ala Pro Asn Tyr 
    50                  55                  60                  


Gly Leu Leu Leu Thr Asp Lys Val Arg Tyr Asp Ile Cys Cys Pro Arg 
65                  70                  75                  80  


Gly Pro Ile Leu Ala Leu Glu Ser Thr Gly Asp Thr Thr Val Lys Ile 
                85                  90                  95      


Gln Gly Pro Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys Phe 
            100                 105                 110         


Glu Gly Cys Lys Leu His Ile Thr Val Lys 
        115                 120         


<210>  6
<211>  98
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  6

Val Asp His Ile Val Gly Glu Glu Lys Gly Trp Val Thr Gly Val Asp 
1               5                   10                  15      


Phe Glu Ala Trp Ala Lys Ala Arg Thr Phe Ser Val Gly Asp Gln Leu 
            20                  25                  30          


Val Phe Lys Tyr Ala Pro Asn Tyr Gly Leu Leu Leu Thr Asp Lys Val 
        35                  40                  45              


Arg Tyr Asp Ile Cys Cys Pro Arg Gly Pro Ile Leu Ala Leu Glu Ser 
    50                  55                  60                  


Thr Gly Asp Thr Thr Val Lys Ile Gln Gly Pro Gly Asp Tyr Tyr Phe 
65                  70                  75                  80  


Ile Ser Tyr Arg Pro Tyr Cys Phe Glu Gly Cys Lys Leu His Ile Thr 
                85                  90                  95      


Val Lys 
        


<210>  7
<211>  432
<212>  DNA
<213>  Ambrosia artemisiifolia


<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  CDS
<222>  (1)..(432)

<400>  7
atg gct aac aaa aat ttg tgc atc ttc gtc ctc ata gca aca ata gtg         48
Met Ala Asn Lys Asn Leu Cys Ile Phe Val Leu Ile Ala Thr Ile Val           
1               5                   10                  15                

gct caa gct aca tgg gtt cga gct aag gac tat cag gtt gga gat atg         96
Ala Gln Ala Thr Trp Val Arg Ala Lys Asp Tyr Gln Val Gly Asp Met           
            20                  25                  30                    

gga gat ggt aat tat aaa gga tta ggt tgg tgc gag act act gat ttc        144
Gly Asp Gly Asn Tyr Lys Gly Leu Gly Trp Cys Glu Thr Thr Asp Phe           
        35                  40                  45                        

caa gct tgg ctc aag ggg aag gag ttc ttt gag gga gac aga ctt tac        192
Gln Ala Trp Leu Lys Gly Lys Glu Phe Phe Glu Gly Asp Arg Leu Tyr           
    50                  55                  60                            

ttc ggt tac aaa gcg gga gaa cat aat gta gtg atg cta aaa gat aaa        240
Phe Gly Tyr Lys Ala Gly Glu His Asn Val Val Met Leu Lys Asp Lys           
65                  70                  75                  80            

gcc gcg tta gac aca tgt act ctt act aat cca caa ttt gca acc cct        288
Ala Ala Leu Asp Thr Cys Thr Leu Thr Asn Pro Gln Phe Ala Thr Pro           
                85                  90                  95                

tct tgt gga tat aat tta gtc act tta ggc gcg gca ccc tat tac atc        336
Ser Cys Gly Tyr Asn Leu Val Thr Leu Gly Ala Ala Pro Tyr Tyr Ile           
            100                 105                 110                   

acc agt ggc atg aat gat gat tgt aag aaa ggc atg aag ttg gaa atc        384
Thr Ser Gly Met Asn Asp Asp Cys Lys Lys Gly Met Lys Leu Glu Ile           
        115                 120                 125                       

aat gcc aag ccg agt cct gca ttg ctg aaa gga aga agc atg aag ctt        432
Asn Ala Lys Pro Ser Pro Ala Leu Leu Lys Gly Arg Ser Met Lys Leu           
    130                 135                 140                           


<210>  8
<211>  144
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  8

Met Ala Asn Lys Asn Leu Cys Ile Phe Val Leu Ile Ala Thr Ile Val 
1               5                   10                  15      


Ala Gln Ala Thr Trp Val Arg Ala Lys Asp Tyr Gln Val Gly Asp Met 
            20                  25                  30          


Gly Asp Gly Asn Tyr Lys Gly Leu Gly Trp Cys Glu Thr Thr Asp Phe 
        35                  40                  45              


Gln Ala Trp Leu Lys Gly Lys Glu Phe Phe Glu Gly Asp Arg Leu Tyr 
    50                  55                  60                  


Phe Gly Tyr Lys Ala Gly Glu His Asn Val Val Met Leu Lys Asp Lys 
65                  70                  75                  80  


Ala Ala Leu Asp Thr Cys Thr Leu Thr Asn Pro Gln Phe Ala Thr Pro 
                85                  90                  95      


Ser Cys Gly Tyr Asn Leu Val Thr Leu Gly Ala Ala Pro Tyr Tyr Ile 
            100                 105                 110         


Thr Ser Gly Met Asn Asp Asp Cys Lys Lys Gly Met Lys Leu Glu Ile 
        115                 120                 125             


Asn Ala Lys Pro Ser Pro Ala Leu Leu Lys Gly Arg Ser Met Lys Leu 
    130                 135                 140                 


<210>  9
<211>  120
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  9

Lys Asp Tyr Gln Val Gly Asp Met Gly Asp Gly Asn Tyr Lys Gly Leu 
1               5                   10                  15      


Gly Trp Cys Glu Thr Thr Asp Phe Gln Ala Trp Leu Lys Gly Lys Glu 
            20                  25                  30          


Phe Phe Glu Gly Asp Arg Leu Tyr Phe Gly Tyr Lys Ala Gly Glu His 
        35                  40                  45              


Asn Val Val Met Leu Lys Asp Lys Ala Ala Leu Asp Thr Cys Thr Leu 
    50                  55                  60                  


Thr Asn Pro Gln Phe Ala Thr Pro Ser Cys Gly Tyr Asn Leu Val Thr 
65                  70                  75                  80  


Leu Gly Ala Ala Pro Tyr Tyr Ile Thr Ser Gly Met Asn Asp Asp Cys 
                85                  90                  95      


Lys Lys Gly Met Lys Leu Glu Ile Asn Ala Lys Pro Ser Pro Ala Leu 
            100                 105                 110         


Leu Lys Gly Arg Ser Met Lys Leu 
        115                 120 


<210>  10
<211>  366
<212>  DNA
<213>  Ambrosia artemisiifolia


<220>
<221>  sig_peptide
<222>  (1)..(72)

<220>
<221>  CDS
<222>  (1)..(366)

<400>  10
atg gct aac aaa agt ttg ggc atc ttc atc ctg ata gca aca ata gta         48
Met Ala Asn Lys Ser Leu Gly Ile Phe Ile Leu Ile Ala Thr Ile Val           
1               5                   10                  15                

gct caa gct aca tgg gtt cga gca gtg gac cat ata gtt gga gat gat         96
Ala Gln Ala Thr Trp Val Arg Ala Val Asp His Ile Val Gly Asp Asp           
            20                  25                  30                    

aaa ggt tgg gtc aat aat ttc gac tac gaa gct tgg gcc aaa ggg agg        144
Lys Gly Trp Val Asn Asn Phe Asp Tyr Glu Ala Trp Ala Lys Gly Arg           
        35                  40                  45                        

aca ttc tct gtg gga gac caa ctt gtg ttc aat tac gat ccg aaa tat        192
Thr Phe Ser Val Gly Asp Gln Leu Val Phe Asn Tyr Asp Pro Lys Tyr           
    50                  55                  60                            

ggt cta ttg ctt acg gat aaa gtg cgt tat gac ata tgt tgt cct cgg        240
Gly Leu Leu Leu Thr Asp Lys Val Arg Tyr Asp Ile Cys Cys Pro Arg           
65                  70                  75                  80            

gga cca att ttg gca ttt gaa tct act gga cat acc gtg gcc aaa ata        288
Gly Pro Ile Leu Ala Phe Glu Ser Thr Gly His Thr Val Ala Lys Ile           
                85                  90                  95                

caa ggg ccg ggc gac tat tac ttt att tcg tat agg cct tat tgt ttt        336
Gln Gly Pro Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys Phe           
            100                 105                 110                   

gag aaa tgt aag ctt cac atc act gtc aag                                366
Glu Lys Cys Lys Leu His Ile Thr Val Lys                                   
        115                 120                                           


<210>  11
<211>  122
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  11

Met Ala Asn Lys Ser Leu Gly Ile Phe Ile Leu Ile Ala Thr Ile Val 
1               5                   10                  15      


Ala Gln Ala Thr Trp Val Arg Ala Val Asp His Ile Val Gly Asp Asp 
            20                  25                  30          


Lys Gly Trp Val Asn Asn Phe Asp Tyr Glu Ala Trp Ala Lys Gly Arg 
        35                  40                  45              


Thr Phe Ser Val Gly Asp Gln Leu Val Phe Asn Tyr Asp Pro Lys Tyr 
    50                  55                  60                  


Gly Leu Leu Leu Thr Asp Lys Val Arg Tyr Asp Ile Cys Cys Pro Arg 
65                  70                  75                  80  


Gly Pro Ile Leu Ala Phe Glu Ser Thr Gly His Thr Val Ala Lys Ile 
                85                  90                  95      


Gln Gly Pro Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys Phe 
            100                 105                 110         


Glu Lys Cys Lys Leu His Ile Thr Val Lys 
        115                 120         


<210>  12
<211>  98
<212>  PRT
<213>  Ambrosia artemisiifolia

<400>  12

Val Asp His Ile Val Gly Asp Asp Lys Gly Trp Val Asn Asn Phe Asp 
1               5                   10                  15      


Tyr Glu Ala Trp Ala Lys Gly Arg Thr Phe Ser Val Gly Asp Gln Leu 
            20                  25                  30          


Val Phe Asn Tyr Asp Pro Lys Tyr Gly Leu Leu Leu Thr Asp Lys Val 
        35                  40                  45              


Arg Tyr Asp Ile Cys Cys Pro Arg Gly Pro Ile Leu Ala Phe Glu Ser 
    50                  55                  60                  


Thr Gly His Thr Val Ala Lys Ile Gln Gly Pro Gly Asp Tyr Tyr Phe 
65                  70                  75                  80  


Ile Ser Tyr Arg Pro Tyr Cys Phe Glu Lys Cys Lys Leu His Ile Thr 
                85                  90                  95      


Val Lys 
        


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 35-49 of precursor protein A

<400>  13

Gly Trp Ala Leu Gly Val Asp Tyr Val Ala Trp Ala Asn Ala Arg 
1               5                   10                  15  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 50-64 of precursor protein A

<400>  14

Gln Ile Val Gln Gly Asp Glu Leu Val Phe Asn Tyr Asp Val Lys 
1               5                   10                  15  


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 65-74 of pro-protein A

<400>  15

Gly Asp Phe Pro Val Phe Tyr Thr Thr Lys 
1               5                   10  


<210>  16
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 80-110 of pro-protein A

<400>  16

Cys Cys Pro Tyr Gly Ala Leu Met Glu Leu Ala Asn Thr Gly His Asn 
1               5                   10                  15      


Val Val Thr Leu Gly Gly Val Gly Asp Tyr Tyr Phe Ile Pro Lys 
            20                  25                  30      


<210>  17
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 34-46 of pro-protein B

<400>  17

Gly Trp Val Thr Gly Val Asp Phe Glu Ala Trp Ala Lys 
1               5                   10              


<210>  18
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 49-59 of pro-protein B

<400>  18

Thr Phe Ser Val Gly Asp Gln Leu Val Phe Lys 
1               5                   10      


<210>  19
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 60-71 of pro-protein B

<400>  19

Tyr Ala Pro Asn Tyr Gly Leu Leu Leu Thr Asp Lys 
1               5                   10          


<210>  20
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 60-73 of pro-protein B

<400>  20

Tyr Ala Pro Asn Tyr Gly Leu Leu Leu Thr Asp Lys Val Arg 
1               5                   10                  


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 81-95 of pro-protein B

<400>  21

Gly Pro Ile Leu Ala Leu Glu Ser Thr Gly Asp Thr Thr Val Lys 
1               5                   10                  15  


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide 96-116 of pro-protein B

<400>  22

Ile Gln Gly Pro Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys 
1               5                   10                  15      


Phe Glu Gly Cys Lys 
            20      


<210>  23
<211>  438
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence cloned in the pET 29 vector to produce the rProtein A 
       (HisCter)


<220>
<221>  CDS
<222>  (16)..(417)

<400>  23
gaaggagata tacat atg gtg gat cat att gtt ggc gac gac aaa ggc tgg        51
                 Met Val Asp His Ile Val Gly Asp Asp Lys Gly Trp          
                 1               5                   10                   

gtt aac aac ttc gac tac gaa gcc tgg gca aaa ggt cgt acc ttt agc         99
Val Asn Asn Phe Asp Tyr Glu Ala Trp Ala Lys Gly Arg Thr Phe Ser           
        15                  20                  25                        

gtt ggc gat cag ctg gtc ttc aac tac gac ccg aaa tac ggt ctg ctg        147
Val Gly Asp Gln Leu Val Phe Asn Tyr Asp Pro Lys Tyr Gly Leu Leu           
    30                  35                  40                            

ctg acc gat aaa gtt cgt tac gac att tgc tgt ccg cgc ggt ccg att        195
Leu Thr Asp Lys Val Arg Tyr Asp Ile Cys Cys Pro Arg Gly Pro Ile           
45                  50                  55                  60            

tta gca ttt gaa agc acc ggt cat acc gtt gca aaa atc cag ggt ccg        243
Leu Ala Phe Glu Ser Thr Gly His Thr Val Ala Lys Ile Gln Gly Pro           
                65                  70                  75                

ggc gat tac tac ttc att agc tac cgc ccg tac tgc ttc gag aaa tgc        291
Gly Asp Tyr Tyr Phe Ile Ser Tyr Arg Pro Tyr Cys Phe Glu Lys Cys           
            80                  85                  90                    

aaa ctg cac atc acc gtt aaa ggc agc ggt acc ctg gtg cca cgc ggt        339
Lys Leu His Ile Thr Val Lys Gly Ser Gly Thr Leu Val Pro Arg Gly           
        95                  100                 105                       

tcc atg gcg ata tcg gat ccg aat tcg agc tcc gtc gac aag ctt gcg        387
Ser Met Ala Ile Ser Asp Pro Asn Ser Ser Ser Val Asp Lys Leu Ala           
    110                 115                 120                           

gcc gca ctc gag cac cac cac cac cac cac tgagatccgg ctgctaacaa a        438
Ala Ala Leu Glu His His His His His His                                   
125                 130                                                   


<210>  24
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  24

Met Val Asp His Ile Val Gly Asp Asp Lys Gly Trp Val Asn Asn Phe 
1               5                   10                  15      


Asp Tyr Glu Ala Trp Ala Lys Gly Arg Thr Phe Ser Val Gly Asp Gln 
            20                  25                  30          


Leu Val Phe Asn Tyr Asp Pro Lys Tyr Gly Leu Leu Leu Thr Asp Lys 
        35                  40                  45              


Val Arg Tyr Asp Ile Cys Cys Pro Arg Gly Pro Ile Leu Ala Phe Glu 
    50                  55                  60                  


Ser Thr Gly His Thr Val Ala Lys Ile Gln Gly Pro Gly Asp Tyr Tyr 
65                  70                  75                  80  


Phe Ile Ser Tyr Arg Pro Tyr Cys Phe Glu Lys Cys Lys Leu His Ile 
                85                  90                  95      


Thr Val Lys Gly Ser Gly Thr Leu Val Pro Arg Gly Ser Met Ala Ile 
            100                 105                 110         


Ser Asp Pro Asn Ser Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu 
        115                 120                 125             


His His His His His His 
    130                 


<210>  25
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence cloned in the pET 28 vector to produce the rProtein A 
       (trx)

<400>  25
atgggcagcc accatcacca tcatcactct ggtatgagcg acaagatcat ccacctgacc       60

gacgattcct tcgacaccga tgtcctgaag gcagatggtg caatcctggt ggatttctgg      120

gcagagtggt gtggtccttg taagatgatc gctccaattc tggacgagat cgcagacgag      180

taccaaggta aactgaccgt ggcaaagctg aacatcgacc agaacccagg taccgctccg      240

aaatacggta tccgtggtat cccgactctg ctgctgttta aaaacggtga agtcgcggct      300

actaaagtgg gcgcactgtc taaaggtcag ctgaaagaat tcctggacgc gaacctggct      360

ctggaagtgc tgttccaagg tccggctact cagcacattg ttggtgacga caaaggttgg      420

gctctgggcg ttgattacgt agcgtgggcc aatgcccgtc agattgttca gggcgatgaa      480

ctggttttta actacgatgt taaaggcgac ttcccggttt tctacacgac caaagacaaa      540

ttcgaacgct gctgcccgta tggcgcgctg atggaactgg cgaacaccgg ccacaacgta      600

gttacgctgg gcggcgttgg cgattattat tttatcccga aaggcgtaga ttgcaaacag      660

aacatgaaac tgcacgtgaa cgtaaaagcg tccccggcgc tgcaggaagg ccgtaatgcc      720

attctggcct aa                                                          732


<210>  26
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rProtein A (trx).

<400>  26

Met Gly Ser His His His His His His Ser Gly Met Ser Asp Lys Ile 
1               5                   10                  15      


Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp Val Leu Lys Ala Asp 
            20                  25                  30          


Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp Cys Gly Pro Cys Lys 
        35                  40                  45              


Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp Glu Tyr Gln Gly Lys 
    50                  55                  60                  


Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn Pro Gly Thr Ala Pro 
65                  70                  75                  80  


Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu Leu Phe Lys Asn Gly 
                85                  90                  95      


Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser Lys Gly Gln Leu Lys 
            100                 105                 110         


Glu Phe Leu Asp Ala Asn Leu Ala Leu Glu Val Leu Phe Gln Gly Pro 
        115                 120                 125             


Ala Thr Gln His Ile Val Gly Asp Asp Lys Gly Trp Ala Leu Gly Val 
    130                 135                 140                 


Asp Tyr Val Ala Trp Ala Asn Ala Arg Gln Ile Val Gln Gly Asp Glu 
145                 150                 155                 160 


Leu Val Phe Asn Tyr Asp Val Lys Gly Asp Phe Pro Val Phe Tyr Thr 
                165                 170                 175     


Thr Lys Asp Lys Phe Glu Arg Cys Cys Pro Tyr Gly Ala Leu Met Glu 
            180                 185                 190         


Leu Ala Asn Thr Gly His Asn Val Val Thr Leu Gly Gly Val Gly Asp 
        195                 200                 205             


Tyr Tyr Phe Ile Pro Lys Gly Val Asp Cys Lys Gln Asn Met Lys Leu 
    210                 215                 220                 


His Val Asn Val Lys Ala Ser Pro Ala Leu Gln Glu Gly Arg Asn Ala 
225                 230                 235                 240 


Ile Leu Ala 
            


