               SEQUENCE LISTING

<110> Agenzia nazionale per le nuove tecnologie, l'energia e lo sviluppo economico sostenibile (ENEA)
<120> Una nuova carotenoide diossigenasi e metodi per la produzione biotecnologica in microorganismi e piante di  composti ad elevato valore aggiunto derivati dallo fferanoUna nuova carotenoide diossigenasi e metodi per la produzione biotecnologica in
microorganismi e piante di composti ad elevato valore aggiunto derivati dallo
zafferano

<130> BW820R

<150> IT102014902281465
<151> 2014-07-23

<160> 2

<170> BiSSAP 1.3

<210> 1
<211> 1688
<212> DNA
<213> Crocus sativus

<400> 1
atggcaaata aggaggaggc agagaagagg aagaagaagc ccaaaccatt aaaagtacta      60

attaccaaag tggatccgaa gccgaggaag ggcatggcat ccgtcgcagt ggacttactc     120

gagaaggcct ttgtatacct attgtccgga aattctgcag ctgatcgtag tagtagtagt     180

ggtcgtcgtc gtcgtaaaga gcattactac ctctccggca attatgcgcc cgtcggacac     240

gaaaccccgc cctccgacca cctccccatt catggatccc ttcctgaatg cttgaatgga     300

gtgtttctga gagttggtcc taaccccaag tttgctcccg tagccggata caattgggtc     360

gatggagatg gaatgattca tggattgcgt attaaagatg gaaaagcaac ttatctatct     420

cgatatatta aaacgtcacg gtttaaacaa gaagaatatt ttggaagagc aaaatttatg     480

aagattggag atctaagggg attgcttggg ttctttacga tcttaatact agtacttcga     540

acaacattga aagtaataga catttcatat ggaagaggga cgggtaatac agctcttgtg     600

tatcataatg gcttactatt ggctctatca gaagaagata aaccttatgt tgttaaagtt     660

ttagaagatg gagacttgca aactcttggg atattggatt atgacaagaa attgtcacat     720

ccattcaccg ctcatccaaa gatcgatccg ttaactgatg agatgtttac ctttggatat     780

tccatctcgc ctccgtatct tacttatcga gtcatttcca aggatggagt gatgcaagat     840

ccagtgcaaa tctcaattac atcccctacc ataatgcatg attttgctat tactgaaaat     900

tatgccatct tcatggacct gcccttgtat ttccaaccag aggaaatggt aaaggggaaa     960

tttgtctctt catttcaccc tacaaaaaga gctcgtatcg gtgtgcttcc acgatatgca    1020

aaagacgagc atccaattcg atggttcgat cttccaagtt gcttcatgac tcataatgca    1080

aatgcttggg aagagaatga tgaagttgtg ctattcacat gtcgccttga gagtcctgat    1140

cttgacatgc ttagtggacc tgcggaagaa gagattggga attcaaaaag tgagctttac    1200

gaaatgaggt tcaatttgaa aactggaatt acttcacaaa agcaactatc tgtacctagt    1260

gttgattttc ctcggatcaa ccaaagttat actggcagga aacagcaata tgtttattgt    1320

actcttggca acaccaagat taagggcatt gtgaagtttg atctgcaaat tgaaccagaa    1380

gccggaaaga caatgcttga agttggagga aatgtacaag gcatctttga gttgggacct    1440

agaagatatg gttcagaggc aatatttgtg ccatgccaac ctggcatcaa atctgatgag    1500

gatgacggtt acttgatatt ctttgtacac gacgaaaaca atgggaaatc tgaggtcaat    1560

gtcatcgatg caaagacaat gtctgcagaa cctgtggctg ttgtggaact tccaagcagg    1620

gttccatatg gattccatgc cttgtttctg aatgaggaag aacttcagaa gcaccaagca    1680

gagacatga                                                            1689


<210> 2
<211> 1123
<212> PRT
<213> Crocus sativus

<400> 2
Met Ala Asn Lys Glu Glu Ala Glu Lys Arg Lys Lys Lys Pro Lys Pro 
1               5                   10                  15      
Leu Lys Val Leu Ile Thr Lys Val Asp Pro Lys Pro Arg Lys Gly Met 
            20                  25                  30          
Ala Ser Val Ala Val Asp Leu Leu Glu Lys Ala Phe Val Tyr Leu Leu 
        35                  40                  45              
Ser Gly Asn Ser Ala Ala Asp Arg Ser Ser Ser Ser Gly Arg Arg Arg 
    50                  55                  60                  
Arg Lys Glu His Tyr Tyr Leu Ser Gly Asn Tyr Ala Pro Val Gly His 
65                  70                  75                  80  
Glu Thr Pro Pro Ser Asp His Leu Pro Ile His Gly Ser Leu Pro Glu 
                85                  90                  95      
Cys Leu Asn Gly Val Phe Leu Arg Val Gly Pro Asn Pro Lys Phe Ala 
            100                 105                 110         
Pro Val Ala Gly Tyr Asn Trp Val Asp Gly Asp Gly Met Ile His Gly 
        115                 120                 125             
Leu Arg Ile Lys Asp Gly Lys Ala Thr Tyr Leu Ser Arg Tyr Ile Lys 
    130                 135                 140                 
Thr Ser Arg Phe Gln Glu Glu Tyr Phe Gly Arg Ala Lys Phe Met Lys 
145                 150                 155                 160 
Ile Gly Asp Leu Arg Gly Leu Leu Gly Phe Phe Thr Ile Leu Ile Leu 
                165                 170                 175     
Val Leu Arg Thr Thr Leu Lys Val Ile Asp Ile Ser Tyr Gly Arg Gly 
            180                 185                 190         
Thr Gly Asn Thr Ala Leu Val Tyr His Asn Gly Leu Leu Leu Ala Leu 
        195                 200                 205             
Ser Glu Glu Asp Lys Pro Tyr Val Val Lys Val Leu Glu Asp Gly Asp 
    210                 215                 220                 
Leu Gln Thr Leu Gly Ile Leu Asp Tyr Asp Lys Lys Leu Ser His Pro 
225                 230                 235                 240 
Phe Thr Ala His Pro Lys Ile Asp Pro Leu Thr Asp Glu Met Phe Thr 
                245                 250                 255     
Phe Gly Tyr Ser Ile Ser Pro Pro Tyr Leu Thr Tyr Arg Val Ile Ser 
            260                 265                 270         
Lys Asp Gly Val Met Gln Asp Pro Val Gln Ile Ser Ile Thr Ser Pro 
        275                 280                 285             
Thr Ile Met His Asp Phe Ala Ile Thr Glu Asn Tyr Ala Ile Phe Met 
    290                 295                 300                 
Asp Leu Pro Leu Tyr Phe Gln Pro Glu Glu Met Val Lys Gly Lys Phe 
305                 310                 315                 320 
Val Ser Ser Phe His Pro Thr Lys Arg Ala Arg Ile Gly Val Leu Pro 
                325                 330                 335     
Arg Tyr Ala Lys Asp Glu His Pro Ile Arg Trp Phe Asp Leu Pro Ser 
            340                 345                 350         
Cys Phe Met Thr His Asn Ala Asn Ala Trp Glu Glu Asn Asp Glu Val 
        355                 360                 365             
Val Leu Phe Thr Cys Arg Leu Glu Ser Pro Asp Leu Asp Met Leu Ser 
    370                 375                 380                 
Gly Pro Ala Glu Glu Glu Ile Gly Asn Ser Lys Ser Glu Leu Tyr Glu 
385                 390                 395                 400 
Met Arg Phe Asn Leu Lys Thr Gly Ile Thr Ser Gln Lys Gln Leu Ser 
                405                 410                 415     
Val Pro Ser Val Asp Phe Pro Arg Ile Asn Gln Ser Tyr Thr Gly Arg 
            420                 425                 430         
Lys Gln Gln Tyr Val Tyr Cys Thr Leu Gly Asn Thr Lys Ile Lys Gly 
        435                 440                 445             
Ile Val Lys Phe Asp Leu Gln Ile Glu Pro Glu Ala Gly Lys Thr Met 
    450                 455                 460                 
Leu Glu Val Gly Gly Asn Val Gln Gly Ile Phe Glu Leu Gly Pro Arg 
465                 470                 475                 480 
Arg Tyr Gly Ser Glu Ala Ile Phe Val Pro Cys Gln Pro Gly Ile Lys 
                485                 490                 495     
Ser Asp Glu Asp Asp Gly Tyr Leu Ile Phe Phe Val His Asp Glu Asn 
            500                 505                 510         
Asn Gly Lys Ser Glu Val Asn Val Ile Asp Ala Lys Thr Met Ser Ala 
        515                 520                 525             
Glu Pro Val Ala Val Val Glu Leu Pro Ser Arg Val Pro Tyr Gly Phe 
    530                 535                 540                 
His Ala Leu Phe Leu Asn Glu Glu Glu Leu Gln Lys His Gln Ala Glu 
545                 550                 555                 560 
Thr Met Ala Asn Lys Glu Glu Ala Glu Lys Arg Lys Lys Lys Pro Lys 
                565                 570                 575     
Pro Leu Lys Val Leu Ile Thr Lys Val Asp Pro Lys Pro Arg Lys Gly 
            580                 585                 590         
Met Ala Ser Val Ala Val Asp Leu Leu Glu Lys Ala Phe Val Tyr Leu 
        595                 600                 605             
Leu Ser Gly Asn Ser Ala Ala Asp Arg Ser Ser Ser Ser Gly Arg Arg 
    610                 615                 620                 
Arg Arg Lys Glu His Tyr Tyr Leu Ser Gly Asn Tyr Ala Pro Val Gly 
625                 630                 635                 640 
His Glu Thr Pro Pro Ser Asp His Leu Pro Ile His Gly Ser Leu Pro 
                645                 650                 655     
Glu Cys Leu Asn Gly Val Phe Leu Arg Val Gly Pro Asn Pro Lys Phe 
            660                 665                 670         
Ala Pro Val Ala Gly Tyr Asn Trp Val Asp Gly Asp Gly Met Ile His 
        675                 680                 685             
Gly Leu Arg Ile Lys Asp Gly Lys Ala Thr Tyr Leu Ser Arg Tyr Ile 
    690                 695                 700                 
Lys Thr Ser Arg Phe Lys Gln Glu Glu Tyr Phe Gly Arg Ala Lys Phe 
705                 710                 715                 720 
Met Lys Ile Gly Asp Leu Arg Gly Leu Leu Gly Phe Phe Thr Ile Leu 
                725                 730                 735     
Ile Leu Val Leu Arg Thr Thr Leu Lys Val Ile Asp Ile Ser Tyr Gly 
            740                 745                 750         
Arg Gly Thr Gly Asn Thr Ala Leu Val Tyr His Asn Gly Leu Leu Leu 
        755                 760                 765             
Ala Leu Ser Glu Glu Asp Lys Pro Tyr Val Val Lys Val Leu Glu Asp 
    770                 775                 780                 
Gly Asp Leu Gln Thr Leu Gly Ile Leu Asp Tyr Asp Lys Lys Leu Ser 
785                 790                 795                 800 
His Pro Phe Thr Ala His Pro Lys Ile Asp Pro Leu Thr Asp Glu Met 
                805                 810                 815     
Phe Thr Phe Gly Tyr Ser Ile Ser Pro Pro Tyr Leu Thr Tyr Arg Val 
            820                 825                 830         
Ile Ser Lys Asp Gly Val Met Gln Asp Pro Val Gln Ile Ser Ile Thr 
        835                 840                 845             
Ser Pro Thr Ile Met His Asp Phe Ala Ile Thr Glu Asn Tyr Ala Ile 
    850                 855                 860                 
Phe Met Asp Leu Pro Leu Tyr Phe Gln Pro Glu Glu Met Val Lys Gly 
865                 870                 875                 880 
Lys Phe Val Ser Ser Phe His Pro Thr Lys Arg Ala Arg Ile Gly Val 
                885                 890                 895     
Leu Pro Arg Tyr Ala Lys Asp Glu His Pro Ile Arg Trp Phe Asp Leu 
            900                 905                 910         
Pro Ser Cys Phe Met Thr His Asn Ala Asn Ala Trp Glu Glu Asn Asp 
        915                 920                 925             
Glu Val Val Leu Phe Thr Cys Arg Leu Glu Ser Pro Asp Leu Asp Met 
    930                 935                 940                 
Leu Ser Gly Pro Ala Glu Glu Glu Ile Gly Asn Ser Lys Ser Glu Leu 
945                 950                 955                 960 
Tyr Glu Met Arg Phe Asn Leu Lys Thr Gly Ile Thr Ser Gln Lys Gln 
                965                 970                 975     
Leu Ser Val Pro Ser Val Asp Phe Pro Arg Ile Asn Gln Ser Tyr Thr 
            980                 985                 990         
Gly Arg Lys Gln Gln Tyr Val Tyr Cys Thr Leu Gly Asn Thr Lys Ile 
        995                 1000                1005            
Lys Gly Ile Val Lys Phe Asp Leu Gln Ile Glu Pro Glu Ala Gly Lys 
    1010                1015                1020                
Thr Met Leu Glu Val Gly Gly Asn Val Gln Gly Ile Phe Glu Leu Gly 
1025                1030                1035                1040
Pro Arg Arg Tyr Gly Ser Glu Ala Ile Phe Val Pro Cys Gln Pro Gly 
                1045                1050                1055    
Ile Lys Ser Asp Glu Asp Asp Gly Tyr Leu Ile Phe Phe Val His Asp 
            1060                1065                1070        
Glu Asn Asn Gly Lys Ser Glu Val Asn Val Ile Asp Ala Lys Thr Met 
        1075                1080                1085            
Ser Ala Glu Pro Val Ala Val Val Glu Leu Pro Ser Arg Val Pro Tyr 
    1090                1095                1100                
Gly Phe His Ala Leu Phe Leu Asn Glu Glu Glu Leu Gln Lys His Gln 
1105                1110                1115                1120
Ala Glu Thr 
            

