                         SEQUENCE LISTING

<110>  PROMEGA CORPORATION
 
<120>  NOVEL LUCIFERASE SEQUENCES UTILIZING INFRARED-EMITTING SUBSTRATES
       TO PRODUCE ENHANCED LUMINESCENCE

<130>  016026-9569-WO00

<140>  New PCT International Patent Application
<141>  2015-09-11

<150>  US 62/049,150
<151>  2014-09-11

<160>  10    

<170>  PatentIn version 3.3

<210>  1
<211>  543
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide

<400>  1

Met Val Lys Arg Glu Lys Asn Val Ile Tyr Gly Pro Glu Pro Leu His 
1               5                   10                  15      


Pro Leu Glu Asp Leu Thr Ala Gly Glu Met Leu Phe Arg Ala Leu Arg 
            20                  25                  30          


Lys His Ser His Leu Pro Gln Ala Leu Val Asp Val Val Gly Asp Glu 
        35                  40                  45              


Ser Leu Ser Tyr Lys Glu Phe Phe Glu Ala Thr Val Leu Leu Ala Gln 
    50                  55                  60                  


Ser Leu His Asn Cys Gly Tyr Lys Met Asn Asp Val Val Ser Ile Cys 
65                  70                  75                  80  


Ala Glu Asn Asn Thr Arg Phe Phe Ile Pro Val Ile Ala Ala Trp Tyr 
                85                  90                  95      


Ile Gly Met Ile Val Ala Pro Val Asn Glu Ser Tyr Ile Pro Asp Glu 
            100                 105                 110         


Leu Cys Lys Val Met Gly Ile Ser Lys Pro Gln Ile Val Phe Thr Thr 
        115                 120                 125             


Lys Asn Ile Leu Asn Lys Val Leu Glu Val Gln Ser Arg Thr Asn Phe 
    130                 135                 140                 


Ile Lys Arg Ile Ile Ile Leu Asp Thr Val Glu Asn Ile His Gly Cys 
145                 150                 155                 160 


Glu Ser Leu Pro Asn Phe Ile Ser Arg Tyr Ser Asp Gly Asn Ile Ala 
                165                 170                 175     


Asn Phe Lys Pro Leu His Phe Asp Pro Val Glu Gln Val Ala Ala Ile 
            180                 185                 190         


Leu Cys Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Gln Thr 
        195                 200                 205             


His Gln Asn Ile Cys Val Arg Leu Ile His Ala Leu Asp Pro Arg Tyr 
    210                 215                 220                 


Gly Thr Gln Leu Ile Pro Gly Val Thr Val Leu Val Tyr Leu Pro Phe 
225                 230                 235                 240 


Phe His Ala Phe Gly Phe His Ile Thr Leu Gly Tyr Phe Met Val Gly 
                245                 250                 255     


Leu Arg Val Ile Met Phe Arg Arg Phe Asp Gln Glu Ala Phe Leu Lys 
            260                 265                 270         


Ala Ile Gln Asp Tyr Glu Val Arg Ser Val Ile Asn Val Pro Ser Val 
        275                 280                 285             


Ile Leu Phe Leu Ser Lys Ser Pro Leu Val Asp Lys Tyr Asp Leu Ser 
    290                 295                 300                 


Ser Leu Arg Glu Leu Cys Cys Gly Ala Ala Pro Leu Ala Lys Glu Val 
305                 310                 315                 320 


Ala Glu Val Ala Ala Lys Arg Leu Asn Leu Pro Gly Ile Arg Cys Gly 
                325                 330                 335     


Phe Gly Leu Thr Glu Ser Thr Ser Ala Ile Ile Gln Thr Leu Gly Asp 
            340                 345                 350         


Glu Phe Lys Ser Gly Ser Leu Gly Arg Val Thr Pro Leu Met Ala Ala 
        355                 360                 365             


Lys Ile Ala Asp Arg Glu Thr Gly Lys Ala Leu Gly Pro Asn Gln Val 
    370                 375                 380                 


Gly Glu Leu Cys Ile Lys Gly Pro Met Val Ser Lys Gly Tyr Val Asn 
385                 390                 395                 400 


Asn Val Glu Ala Thr Lys Glu Ala Ile Asp Asp Asp Gly Trp Leu His 
                405                 410                 415     


Ser Gly Asp Phe Gly Tyr Tyr Asp Glu Asp Glu His Phe Tyr Val Val 
            420                 425                 430         


Asp Arg Tyr Lys Glu Leu Ile Lys Tyr Lys Gly Ser Gln Val Ala Pro 
        435                 440                 445             


Ala Glu Leu Glu Glu Ile Leu Leu Lys Asn Pro Cys Ile Arg Asp Val 
    450                 455                 460                 


Ala Val Val Gly Ile Pro Asp Leu Glu Ala Gly Glu Leu Pro Ser Ala 
465                 470                 475                 480 


Phe Val Val Lys Gln Pro Gly Thr Glu Ile Thr Ala Lys Glu Val Tyr 
                485                 490                 495     


Asp Tyr Leu Ala Glu Arg Val Ser His Thr Lys Tyr Leu Arg Gly Gly 
            500                 505                 510         


Val Arg Phe Val Asp Ser Ile Pro Arg Asn Val Thr Gly Lys Ile Thr 
        515                 520                 525             


Arg Lys Glu Leu Leu Lys Gln Leu Leu Val Lys Ala Gly Gly Val 
    530                 535                 540             


<210>  2
<211>  543
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide

<400>  2

Met Val Lys Arg Glu Lys Asn Val Ile Tyr Gly Pro Glu Pro Leu His 
1               5                   10                  15      


Pro Leu Glu Asp Leu Thr Ala Gly Glu Met Leu Phe Arg Ala Leu Arg 
            20                  25                  30          


Lys His Ser His Leu Pro Gln Ala Leu Val Asp Val Val Gly Asp Glu 
        35                  40                  45              


Ser Leu Ser Tyr Lys Glu Phe Phe Glu Ala Thr Val Leu Leu Ala Gln 
    50                  55                  60                  


Ser Leu His Asn Cys Gly Tyr Lys Met Asn Asp Val Val Ser Ile Cys 
65                  70                  75                  80  


Ala Glu Asn Asn Thr Arg Phe Phe Ile Pro Val Ile Ala Ala Trp Tyr 
                85                  90                  95      


Ile Gly Met Ile Val Ala Pro Val Asn Glu Ser Tyr Ile Pro Asp Glu 
            100                 105                 110         


Leu Cys Lys Val Met Gly Ile Ser Lys Pro Gln Ile Val Phe Thr Thr 
        115                 120                 125             


Lys Asn Ile Leu Asn Lys Val Leu Glu Val Gln Ser Arg Thr Asn Phe 
    130                 135                 140                 


Ile Lys Arg Ile Ile Ile Leu Asp Thr Val Glu Asn Ile His Gly Cys 
145                 150                 155                 160 


Glu Ser Leu Pro Asn Phe Ile Ser Arg Tyr Ser Asp Gly Asn Ile Ala 
                165                 170                 175     


Asn Phe Lys Pro Leu His Phe Asp Pro Val Glu Gln Val Ala Ala Ile 
            180                 185                 190         


Leu Cys Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Gln Thr 
        195                 200                 205             


His Gln Asn Ile Cys Val Arg Leu Ile His Ala Leu Asp Pro Arg Tyr 
    210                 215                 220                 


Gly Thr Gln Leu Ile Pro Gly Val Thr Val Leu Val Tyr Leu Pro Phe 
225                 230                 235                 240 


Phe His Ala Phe Gly Phe His Ile Thr Leu Gly Tyr Phe Met Val Gly 
                245                 250                 255     


Leu Arg Val Ile Met Phe Arg Arg Phe Asp Gln Glu Ala Phe Leu Lys 
            260                 265                 270         


Ala Ile Gln Asp Tyr Glu Val Arg Ser Val Ile Asn Val Pro Ser Val 
        275                 280                 285             


Ile Leu Phe Leu Ser Lys Ser Pro Leu Val Asp Lys Tyr Asp Leu Ser 
    290                 295                 300                 


Ser Leu Arg Glu Leu Cys Cys Gly Ala Ala Pro Leu Ala Lys Glu Val 
305                 310                 315                 320 


Ala Glu Val Ala Ala Lys Arg Leu Asn Leu Pro Gly Ile Ser Cys Gly 
                325                 330                 335     


Phe Gly Leu Thr Glu Ser Thr Ser Ala Ile Ile Gln Thr Leu Arg Asp 
            340                 345                 350         


Glu Phe Lys Ser Gly Ser Leu Gly Arg Val Thr Pro Leu Met Ala Ala 
        355                 360                 365             


Lys Ile Ala Asp Arg Glu Thr Gly Lys Ala Leu Gly Pro Asn Gln Val 
    370                 375                 380                 


Gly Glu Leu Cys Ile Lys Gly Pro Met Val Ser Lys Gly Tyr Val Asn 
385                 390                 395                 400 


Asn Val Glu Ala Thr Lys Glu Ala Ile Asp Asp Asp Gly Trp Leu His 
                405                 410                 415     


Ser Gly Asp Phe Gly Tyr Tyr Asp Glu Asp Glu His Phe Tyr Val Val 
            420                 425                 430         


Asp Arg Tyr Lys Glu Leu Ile Lys Tyr Lys Gly Ser Gln Val Ala Pro 
        435                 440                 445             


Ala Glu Leu Glu Glu Ile Leu Leu Lys Asn Pro Cys Ile Arg Asp Val 
    450                 455                 460                 


Ala Val Val Gly Ile Pro Asp Leu Glu Ala Gly Glu Leu Pro Ser Ala 
465                 470                 475                 480 


Phe Val Val Lys Gln Pro Gly Thr Glu Ile Thr Ala Lys Glu Val Tyr 
                485                 490                 495     


Asp Tyr Leu Ala Glu Arg Val Ser His Thr Lys Tyr Leu Arg Gly Gly 
            500                 505                 510         


Val Arg Phe Val Asp Ser Ile Pro Arg Asn Val Thr Gly Lys Ile Thr 
        515                 520                 525             


Arg Lys Glu Leu Leu Lys Gln Leu Leu Val Lys Ala Gly Gly Val 
    530                 535                 540             


<210>  3
<211>  543
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide

<400>  3

Met Val Lys Arg Glu Lys Asn Val Ile Tyr Gly Pro Glu Pro Leu His 
1               5                   10                  15      


Pro Leu Glu Asp Leu Thr Ala Gly Glu Met Leu Phe Arg Ala Leu Arg 
            20                  25                  30          


Lys His Ser His Leu Pro Gln Ala Leu Val Asp Val Val Gly Asp Glu 
        35                  40                  45              


Ser Leu Ser Tyr Lys Glu Phe Phe Glu Ala Thr Val Leu Leu Ala Gln 
    50                  55                  60                  


Ser Leu His Asn Cys Gly Tyr Lys Met Asn Asp Val Val Ser Ile Cys 
65                  70                  75                  80  


Ala Glu Asn Asn Thr Arg Phe Phe Ile Pro Val Ile Ala Ala Trp Tyr 
                85                  90                  95      


Ile Gly Met Ile Val Ala Pro Val Asn Glu Ser Tyr Ile Pro Asp Glu 
            100                 105                 110         


Leu Cys Lys Val Met Gly Ile Ser Lys Pro Gln Ile Val Phe Thr Thr 
        115                 120                 125             


Lys Asn Ile Leu Asn Lys Val Leu Glu Val Gln Ser Arg Thr Asn Phe 
    130                 135                 140                 


Ile Lys Arg Ile Ile Ile Leu Asp Thr Val Glu Asn Ile His Gly Cys 
145                 150                 155                 160 


Glu Ser Leu Pro Asn Phe Ile Ser Arg Tyr Ser Asp Gly Asn Ile Ala 
                165                 170                 175     


Asn Phe Lys Pro Leu His Phe Asp Pro Val Glu Gln Val Ala Ala Ile 
            180                 185                 190         


Leu Cys Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Gln Thr 
        195                 200                 205             


His Gln Asn Ile Cys Val Arg Leu Ile His Ala Leu Asp Pro Arg Tyr 
    210                 215                 220                 


Gly Thr Gln Leu Ile Pro Gly Val Thr Val Leu Val Tyr Leu Pro Phe 
225                 230                 235                 240 


Phe His Ala Phe Gly Phe His Ile Thr Leu Ser Tyr Phe Met Val Gly 
                245                 250                 255     


Leu Arg Val Ile Met Phe Arg Arg Phe Asp Gln Glu Ala Phe Leu Lys 
            260                 265                 270         


Ala Ile Gln Asp Tyr Glu Val Arg Ser Val Ile Asn Val Pro Ser Val 
        275                 280                 285             


Ile Leu Phe Leu Ser Lys Ser Pro Leu Val Asp Lys Tyr Asp Leu Ser 
    290                 295                 300                 


Ser Leu Arg Glu Leu Cys Cys Gly Ala Ala Pro Leu Ala Lys Glu Val 
305                 310                 315                 320 


Ala Glu Val Ala Ala Lys Arg Leu Asn Leu Pro Gly Ile Arg Cys Gly 
                325                 330                 335     


Phe Gly Leu Thr Glu Ser Thr Ser Ala Ile Ile Gln Thr Leu Gly Asp 
            340                 345                 350         


Glu Phe Lys Ser Gly Ser Leu Gly Arg Val Thr Pro Leu Met Ala Ala 
        355                 360                 365             


Lys Ile Ala Asp Arg Glu Thr Gly Lys Ala Leu Gly Pro Asn Gln Val 
    370                 375                 380                 


Gly Glu Leu Cys Phe Lys Gly Pro Met Val Ser Lys Gly Tyr Val Asn 
385                 390                 395                 400 


Asn Val Glu Ala Thr Lys Glu Ala Ile Asp Asp Asp Gly Trp Leu His 
                405                 410                 415     


Ser Gly Asp Phe Gly Tyr Tyr Asp Glu Asp Glu His Phe Tyr Val Val 
            420                 425                 430         


Asp Arg Tyr Lys Glu Leu Ile Lys Tyr Lys Gly Arg Gln Val Ala Pro 
        435                 440                 445             


Ala Glu Leu Glu Glu Ile Leu Leu Lys Asn Pro Cys Ile Arg Asp Val 
    450                 455                 460                 


Ala Val Val Gly Ile Pro Asp Leu Glu Ala Gly Glu Leu Pro Ser Ala 
465                 470                 475                 480 


Phe Val Val Lys Gln Pro Gly Thr Glu Ile Thr Ala Lys Glu Val Tyr 
                485                 490                 495     


Asp Tyr Leu Ala Glu Arg Val Ser His Thr Lys Tyr Leu Arg Gly Gly 
            500                 505                 510         


Val Arg Phe Val Asp Ser Ile Pro Arg Asn Val Thr Gly Lys Ile Thr 
        515                 520                 525             


Arg Lys Glu Leu Leu Lys Gln Leu Leu Val Lys Ala Gly Gly Val 
    530                 535                 540             


<210>  4
<211>  543
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Peptide

<400>  4

Met Val Lys Arg Glu Lys Asn Val Ile Tyr Gly Pro Glu Pro Leu His 
1               5                   10                  15      


Pro Leu Glu Asp Leu Thr Ala Gly Glu Met Leu Phe Arg Ala Leu Arg 
            20                  25                  30          


Lys His Ser His Leu Pro Gln Ala Leu Val Asp Val Val Gly Asp Glu 
        35                  40                  45              


Ser Leu Asn Tyr Lys Glu Phe Phe Glu Ala Thr Val Leu Leu Ala Gln 
    50                  55                  60                  


Ser Leu His Asn Cys Gly Tyr Lys Met Asn Asp Val Val Ser Ile Cys 
65                  70                  75                  80  


Ala Glu Asn Asn Thr Arg Phe Phe Ile Pro Val Ile Ala Ala Trp Tyr 
                85                  90                  95      


Ile Gly Met Ile Val Ala Pro Val Asn Glu Ser Tyr Ile Pro Asp Glu 
            100                 105                 110         


Leu Cys Lys Val Met Gly Ile Ser Lys Pro Gln Ile Val Phe Thr Thr 
        115                 120                 125             


Lys Asn Ile Leu Asn Lys Val Leu Glu Val Gln Ser Arg Thr Asn Phe 
    130                 135                 140                 


Ile Lys Arg Ile Ile Ile Leu Asp Thr Val Glu Asn Ile His Gly Cys 
145                 150                 155                 160 


Glu Ser Leu Pro Asn Phe Ile Ser Arg Tyr Ser Asp Gly Asn Ile Ala 
                165                 170                 175     


Asn Phe Lys Pro Leu His Phe Asp Pro Val Glu Gln Val Ala Ala Ile 
            180                 185                 190         


Leu Cys Ser Ser Gly Thr Thr Gly Leu Pro Lys Gly Val Met Gln Thr 
        195                 200                 205             


His Gln Asn Ile Cys Val Arg Leu Ile His Ala Leu Asp Pro Arg Tyr 
    210                 215                 220                 


Gly Thr Gln Leu Ile Pro Gly Val Thr Val Leu Val Tyr Leu Pro Phe 
225                 230                 235                 240 


Phe His Ala Phe Gly Phe His Ile Thr Leu Gly Tyr Phe Met Val Gly 
                245                 250                 255     


Leu Arg Val Ile Met Phe Arg Arg Phe Asp Gln Glu Ala Phe Leu Lys 
            260                 265                 270         


Ala Ile Gln Asp Tyr Glu Val Arg Ser Val Ile Asn Val Pro Ser Val 
        275                 280                 285             


Ile Leu Phe Leu Ser Lys Ser Pro Leu Val Asp Lys Tyr Asp Leu Ser 
    290                 295                 300                 


Ser Leu Arg Glu Leu Cys Cys Gly Ala Ala Pro Leu Ala Lys Glu Val 
305                 310                 315                 320 


Ala Glu Val Ala Ala Lys Arg Leu Asn Leu Pro Gly Ile Ser Cys Gly 
                325                 330                 335     


Phe Gly Leu Thr Glu Ser Thr Ser Ala Ile Ile Gln Thr Leu Arg Asp 
            340                 345                 350         


Glu Phe Lys Ser Gly Ser Leu Gly Arg Val Thr Pro Leu Met Ala Ala 
        355                 360                 365             


Lys Ile Ala Asp Arg Glu Thr Gly Lys Ala Leu Gly Pro Asn Gln Val 
    370                 375                 380                 


Gly Glu Leu Cys Ile Lys Gly Pro Met Val Ser Lys Gly Tyr Val Asn 
385                 390                 395                 400 


Asn Val Glu Ala Thr Lys Glu Ala Ile Asp Asp Asp Gly Trp Leu His 
                405                 410                 415     


Ser Gly Asp Phe Gly Tyr Tyr Asp Glu Asp Glu His Phe Tyr Val Val 
            420                 425                 430         


Asp Arg Tyr Lys Glu Leu Ile Lys Tyr Lys Gly Arg Gln Val Ala Pro 
        435                 440                 445             


Ala Glu Leu Glu Glu Ile Leu Leu Lys Asn Pro Cys Ile Arg Asp Val 
    450                 455                 460                 


Ala Val Val Gly Ile Pro Asp Leu Glu Ala Gly Glu Leu Pro Ser Ala 
465                 470                 475                 480 


Phe Val Val Lys Gln Pro Gly Thr Glu Ile Thr Ala Lys Glu Val Tyr 
                485                 490                 495     


Asp Tyr Leu Ala Glu Arg Val Ser His Thr Lys Tyr Leu Arg Gly Gly 
            500                 505                 510         


Val Arg Phe Val Asp Ser Ile Pro Arg Asn Val Thr Gly Lys Ile Thr 
        515                 520                 525             


Arg Lys Glu Leu Leu Lys Gln Leu Leu Val Lys Ala Gly Gly Val 
    530                 535                 540             


<210>  5
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; Variant ATG 343 = CBR sequence (amino 
       acid sequence SEQ ID NO:1)

<400>  5
atggtaaagc gtgagaaaaa tgtcatctat ggccctgagc ctctccatcc tttggaggat     60

ttgactgccg gcgaaatgct gtttcgtgct ctccgcaagc actctcattt gcctcaagcc    120

ttggtcgatg tggtcggcga tgaatctttg agctacaagg agttttttga ggcaaccgtc    180

ttgctggctc agtccctcca caattgtggc tacaagatga acgacgtcgt tagtatctgt    240

gctgaaaaca atacccgttt cttcattcca gtcatcgccg catggtatat cggtatgatc    300

gtggctccag tcaacgagag ctacattccc gacgaactgt gtaaagtcat gggtatctct    360

aagccacaga ttgtcttcac cactaagaat attctgaaca aagtcctgga agtccaaagc    420

cgcaccaact ttattaagcg tatcatcatc ttggacactg tggagaatat tcacggttgc    480

gaatctttgc ctaatttcat ctctcgctat tcagacggca acatcgcaaa ctttaaacca    540

ctccacttcg accctgtgga acaagttgca gccattctgt gtagcagcgg tactactgga    600

ctcccaaagg gagtcatgca gacccatcaa aacatttgcg tgcgtctgat ccatgctctc    660

gatccacgct acggcactca gctgattcct ggtgtcaccg tcttggtcta cttgcctttc    720

ttccatgctt tcggctttca tattactttg ggttacttta tggtcggtct ccgcgtgatt    780

atgttccgcc gttttgatca ggaggctttc ttgaaagcca tccaagatta tgaagtccgc    840

agtgtcatca acgtgcctag cgtgatcctg tttttgtcta agagcccact cgtggacaag    900

tacgacttgt cttcactgcg tgaattgtgt tgcggtgccg ctccactggc taaggaggtc    960

gctgaagtgg ccgccaaacg cttgaatctt ccagggattc gttgtggctt cggcctcacc   1020

gaatctacca gtgcgattat ccagactctc ggggatgagt ttaagagcgg ctctttgggc   1080

cgtgtcactc cactcatggc tgctaagatc gctgatcgcg aaactggtaa ggctttgggc   1140

ccgaaccaag tgggcgagct gtgtatcaaa ggccctatgg tgagcaaggg ttatgtcaat   1200

aacgttgaag ctaccaagga ggccatcgac gacgacggct ggttgcattc tggtgatttt   1260

ggatattacg acgaagatga gcatttttac gtcgtggatc gttacaagga gctgatcaaa   1320

tacaagggta gccaggttgc tccagctgag ttggaggaga ttctgttgaa aaatccatgc   1380

attcgcgatg tcgctgtggt cggcattcct gatctggagg ccggcgaact gccttctgct   1440

ttcgttgtca agcagcctgg tacagaaatt accgccaaag aagtgtatga ttacctggct   1500

gaacgtgtga gccatactaa gtacttgcgt ggcggcgtgc gttttgttga ctccatccct   1560

cgtaacgtaa caggcaaaat tacccgcaag gagctgttga aacaattgtt ggtgaaggcc   1620

ggcggtgttt aa                                                       1632


<210>  6
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; Variant ATG 1240 = CBR sequence + 
       R334S/G351R (amino acid sequence SEQ ID NO:2)

<400>  6
atggtaaagc gtgagaaaaa tgtcatctat ggccctgagc ctctccatcc tttggaggat     60

ttgactgccg gcgaaatgct gtttcgtgct ctccgcaagc actctcattt gcctcaagcc    120

ttggtcgatg tggtcggcga tgaatctttg agctacaagg agttttttga ggcaaccgtc    180

ttgctggctc agtccctcca caattgtggc tacaagatga acgacgtcgt tagtatctgt    240

gctgaaaaca atacccgttt cttcattcca gtcatcgccg catggtatat cggtatgatc    300

gtggctccag tcaacgagag ctacattccc gacgaactgt gtaaagtcat gggtatctct    360

aagccacaga ttgtcttcac cactaagaat attctgaaca aagtcctgga agtccaaagc    420

cgcaccaact ttattaagcg tatcatcatc ttggacactg tggagaatat tcacggttgc    480

gaatctttgc ctaatttcat ctctcgctat tcagacggca acatcgcaaa ctttaaacca    540

ctccacttcg accctgtgga acaagttgca gccattctgt gtagcagcgg tactactgga    600

ctcccaaagg gagtcatgca gacccatcaa aacatttgcg tgcgtctgat ccatgctctc    660

gatccacgct acggcactca gctgattcct ggtgtcaccg tcttggtcta cttgcctttc    720

ttccatgctt tcggctttca tattactttg ggttacttta tggtcggtct ccgcgtgatt    780

atgttccgcc gttttgatca ggaggctttc ttgaaagcca tccaagatta tgaagtccgc    840

agtgtcatca acgtgcctag cgtgatcctg tttttgtcta agagcccact cgtggacaag    900

tacgacttgt cttcactgcg tgaattgtgt tgcggtgccg ctccactggc taaggaggtc    960

gctgaagtgg ccgccaaacg cttgaatctt ccagggatta gttgtggctt cggcctcacc   1020

gaatctacca gtgcgattat ccagactctc cgggatgagt ttaagagcgg ctctttgggc   1080

cgtgtcactc cactcatggc tgctaagatc gctgatcgcg aaactggtaa ggctttgggc   1140

ccgaaccaag tgggcgagct gtgtatcaaa ggccctatgg tgagcaaggg ttatgtcaat   1200

aacgttgaag ctaccaagga ggccatcgac gacgacggct ggttgcattc tggtgatttt   1260

ggatattacg acgaagatga gcatttttac gtcgtggatc gttacaagga gctgatcaaa   1320

tacaagggta gccaggttgc tccagctgag ttggaggaga ttctgttgaa aaatccatgc   1380

attcgcgatg tcgctgtggt cggcattcct gatctggagg ccggcgaact gccttctgct   1440

ttcgttgtca agcagcctgg tacagaaatt accgccaaag aagtgtatga ttacctggct   1500

gaacgtgtga gccatactaa gtacttgcgt ggcggcgtgc gttttgttga ctccatccct   1560

cgtaacgtaa caggcaaaat tacccgcaag gagctgttga aacaattgtt ggtgaaggcc   1620

ggcggtgttt aa                                                       1632


<210>  7
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; Variant ATG 1929 = CBR sequence + 
       R334S/G351R (codon optimized for RNA structure and codon usage in
       rodents) (amino acid sequence SEQ ID NO:2)

<400>  7
atggttaaga gagaaaaaaa tgttatttat ggtcctgaac ctctgcatcc tctggaagat     60

ctcactgcag gagagatgct gtttcgcgcc ctgcgcaagc actctcactt gccgcaggct    120

ctggtcgacg tggtgggtga cgagtccctg agctacaagg agttcttcga ggcgactgtc    180

ctgctggcac agagtctcca taactgcggc tacaagatga acgatgtggt ttccatctgc    240

gcagagaaca acactagatt ctttatccca gtgatcgctg cttggtacat cggcatgatt    300

gtcgcaccag tcaatgaatc ttacattcca gatgaactgt gtaaggttat gggaatttcc    360

aaaccgcaga tcgtgttcac aactaagaac atcctgaaca aggttctgga agtgcagagt    420

cggaccaatt tcatcaagcg tattatcatc ctggacacag tggagaacat ccacggttgc    480

gaatccctcc ccaacttcat ctctcgttat agcgatggca acatcgctaa tttcaaaccc    540

ctgcatttcg atcctgttga acaggttgcc gccatcctgt gctcctctgg gaccaccgga    600

ctgccgaaag gcgtgatgca gacccatcag aacatctgcg tgaggctgat ccacgccctg    660

gacccacgct atggaactca gctgatccca ggagtcactg tgctggttta cctccctttt    720

tttcacgcct tcggtttcca cattaccctc gggtacttta tggtcggcct ccgcgttatc    780

atgttccgtc gctttgacca ggaggctttc ctgaaggcca tccaggatta tgaggtgagg    840

agtgtgatta acgtcccaag cgtgatcctg ttcctgtcta agtcccctct ggttgacaag    900

tacgatctga gctccctgcg tgagctgtgc tgcggggctg caccactggc caaagaagtc    960

gcggaagtgg ctgccaaacg cctgaacctg ccgggtatct cctgtggctt cggcctgaca   1020

gagagcacat ccgctatcat ccagacactg cgggatgagt ttaagtccgg ttccctgggc   1080

cgggttacac ccctgatggc agccaagatc gccgaccgcg agactgggaa ggccctcgga   1140

cctaaccagg tcggagagct gtgtattaag ggccccatgg tgtccaaggg atatgtgaac   1200

aacgtggaag ctacaaagga ggcgattgat gatgacggat ggctccatag cggagacttt   1260

gggtactatg acgaggacga gcacttctat gtggtcgaca ggtacaagga gctgatcaag   1320

tacaagggct cccaggtggc cccagctgaa ctggaggaga ttctgttgaa gaacccttgt   1380

atcagagatg tggctgttgt ggggattcct gacctcgaag caggtgagct gccctccgct   1440

ttcgtggtga agcagcctgg tactgagatc accgccaaag aggtgtacga ttacctggct   1500

gagcgcgtgt cccacaccaa atacctccgc ggcggggtcc gtttcgtgga ctccattccc   1560

aggaacgtga ccggcaagat cacccgcaag gagctgctga agcagctgct ggttaaggct   1620

ggcggcgttt aa                                                       1632


<210>  8
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; Variant ATG 1944 = CBR sequence + 
       R334S/G351R (codon optimized for RNA structure and codon usage in
       CHO cells) (amino acid sequence SEQ ID NO:2)

<400>  8
atggtcaaga gagaaaagaa cgtcatctac ggtcccgagc ccctgcaccc cctggaagat     60

ctgactgccg gagagatgct tttccgggct ctgcgcaagc atagccacct cccgcaagca    120

ctggtggacg tggtcgggga cgagtcactg tcgtataagg agttcttcga agcgaccgtg    180

ctgctggccc agtccctgca caactgcgga tacaagatga atgacgtggt gtcgatttgc    240

gccgaaaaca acacccggtt cttcattccc gtgatcgccg cttggtacat cgggatgatc    300

gtggcccccg tcaacgagtc atacatccct gacgagctgt gcaaggtcat gggtatatca    360

aagccccaga tcgtgttcac cactaagaac atcttgaaca aagtgctgga agtgcagtcc    420

cggaccaact ttatcaagcg gatcatcatc ctggataccg tggagaacat ccacggttgt    480

gaatcgctgc ctaacttcat tagccggtac tcggacggca atatcgcaaa cttcaagccc    540

ctccatttcg accctgtgga acaagtggcc gcgatcttgt gttcatcggg caccaccgga    600

ctcccaaagg gggtcatgca gactcatcag aacatttgcg tgagactgat tcacgccctg    660

gaccctcgct acgggactca actcattccg ggagtgaccg tgctcgtgta tttgccgttc    720

ttccacgcct ttggcttcca catcaccctg gggtacttta tggtcggact gcgcgtgatc    780

atgttccgga gatttgacca ggaggcattc ctgaaagcca tccaagacta cgaagtccgg    840

agcgtgatta acgtgccgag cgtgatcttg ttcctgtcca agtccccttt ggtggataag    900

tacgacctgt cgagcctgag agagctctgt tgcggcgcgg ccccgctcgc taaggaagtg    960

gccgaagtgg cggctaagag gctgaacctt cctggaattt cctgcggctt cggcctgacc   1020

gagtccacta gcgcgatcat tcagaccctg cgcgatgagt tcaaatccgg ctctctgggc   1080

cgcgtgactc ctctgatggc cgcaaagatc gcggacaggg aaactgggaa agccctcggt   1140

ccgaatcagg tcggagagct ctgcattaag ggaccaatgg tgtccaaggg atacgtgaac   1200

aacgtggagg ccaccaagga agccatcgac gacgacggat ggctgcattc cggagacttc   1260

gggtactacg acgaggatga gcacttctac gtggtggatc ggtacaagga gcttatcaag   1320

tacaagggct cccaggtcgc tccggccgag ctggaagaaa ttctgctcaa gaacccatgc   1380

atccgagatg tggccgtggt cggtatcccc gacctggagg ccggagaact gccgtccgcc   1440

ttcgtggtca agcagcccgg cacagagatt accgcgaagg aagtgtacga ttacctggcc   1500

gaacgggtgt cccacacgaa gtatcttcgc ggcggagtgc gcttcgtcga ctccatcccg   1560

agaaacgtga ccggaaagat taccaggaag gaactcctga agcagctgct cgtgaaagcc   1620

ggcggagtgt ag                                                       1632


<210>  9
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; Variant ATG 1945 = CBR sequence + 
       R334S/G351R (codon optimized for RNA structure and codon usage in
       mouse lung and liver cells) (amino acid sequence SEQ ID NO:2)

<400>  9
atggttaaga gagaaaagaa cgtgatatac ggtccagaac ccctccaccc acttgaagat     60

cttacagccg gggaaatgtt attccgtgca cttaggaaac atagccattt gccgcaagcc    120

ctggtcgatg tcgtcgggga cgagtctctg agttacaagg agtttttcga agccaccgtg    180

ttgctagctc agtcactcca taactgtggt tacaagatga atgacgtggt gtcaatctgc    240

gctgaaaaca atacccgctt ctttatcccc gtgattgctg cttggtatat cggcatgatt    300

gtggccccgg ttaatgagag ttacatcccc gacgagctgt gtaaagtcat gggaattagc    360

aagccgcaga tagtgtttac cacgaagaac atcctcaaca aggtcctcga ggtccagtct    420

cggaccaatt tcattaagag gattatcatc ctggacaccg tagaaaatat ccatggctgc    480

gagtctcttc ccaactttat ctctcgctat tccgacggaa atatcgcaaa tttcaaacca    540

ctgcattttg acccggtcga acaagtggct gctatcctgt gttcgtccgg tacaacgggc    600

ctacctaagg gcgttatgca gactcatcag aatatctgcg tgcgcttaat tcacgctctt    660

gacccacgtt acggcacaca gttaatcccc ggtgttactg tcctcgtata ccttccattt    720

ttccatgcat tcggctttca tattacactt ggctacttta tggttggcct tcgcgtaatt    780

atgtttcggc gatttgacca ggaagcattt ctgaaagcta ttcaggatta tgaagttcgc    840

tctgtcatca atgtgccaag tgtcatattg tttctgtcta aaagtcccct cgtggacaaa    900

tatgatcttt cttctctccg cgaactgtgc tgcggcgcgg ctccacttgc taaggaagtg    960

gctgaggtcg cagctaagcg cctaaatctt ccgggaatct cctgcggttt tgggcttacc   1020

gaatcaacat ctgccataat tcagacactt agggacgaat tcaagagtgg tagtctgggc   1080

cgcgtgacgc cgcttatggc tgctaagatc gctgacaggg aaactgggaa ggctctgggt   1140

cctaatcaag ttggggaact ttgcattaag ggccctatgg tgtccaaggg atacgtaaac   1200

aatgtcgaag ctacgaagga ggctatcgat gacgatggat ggcttcatag cggcgacttt   1260

gggtactatg acgaggacga acacttttat gtggtggacc gctacaagga actcataaag   1320

tacaagggta gccaggtcgc cccggctgag ctcgaagaaa tccttctcaa aaatccctgt   1380

atccgcgatg tggccgtcgt tggcataccg gatcttgaag ccggagaact tccctctgca   1440

tttgtggtca agcagccagg aaccgaaatc actgcgaagg aggtgtacga ttatctcgcc   1500

gaacgcgtgt ctcacaccaa gtacctaaga ggaggggttc ggtttgtgga tagtatacct   1560

cgcaatgtca caggaaaaat cacccggaaa gagctgttga aacagctgct cgtaaaggcc   1620

ggtggtgtct ga                                                       1632


<210>  10
<211>  1632
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic Oligonucleotide; ATG 1240 + S51N + S444R (amino acid 
       sequence SEQ ID NO: 4)

<400>  10
atggtaaagc gtgagaaaaa tgtcatctat ggccctgagc ctctccatcc tttggaggat     60

ttgactgccg gcgaaatgct gtttcgtgct ctccgcaagc actctcattt gcctcaagcc    120

ttggtcgatg tggtcggcga tgaatctttg aactacaagg agttttttga ggcaaccgtc    180

ttgctggctc agtccctcca caattgtggc tacaagatga acgacgtcgt tagtatctgt    240

gctgaaaaca atacccgttt cttcattcca gtcatcgccg catggtatat cggtatgatc    300

gtggctccag tcaacgagag ctacattccc gacgaactgt gtaaagtcat gggtatctct    360

aagccacaga ttgtcttcac cactaagaat attctgaaca aagtcctgga agtccaaagc    420

cgcaccaact ttattaagcg tatcatcatc ttggacactg tggagaatat tcacggttgc    480

gaatctttgc ctaatttcat ctctcgctat tcagacggca acatcgcaaa ctttaaacca    540

ctccacttcg accctgtgga acaagttgca gccattctgt gtagcagcgg tactactgga    600

ctcccaaagg gagtcatgca gacccatcaa aacatttgcg tgcgtctgat ccatgctctc    660

gatccacgct acggcactca gctgattcct ggtgtcaccg tcttggtcta cttgcctttc    720

ttccatgctt tcggctttca tattactttg ggttacttta tggtcggtct ccgcgtgatt    780

atgttccgcc gttttgatca ggaggctttc ttgaaagcca tccaagatta tgaagtccgc    840

agtgtcatca acgtgcctag cgtgatcctg tttttgtcta agagcccact cgtggacaag    900

tacgacttgt cttcactgcg tgaattgtgt tgcggtgccg ctccactggc taaggaggtc    960

gctgaagtgg ccgccaaacg cttgaatctt ccagggatta gttgtggctt cggcctcacc   1020

gaatctacca gtgcgattat ccagactctc cgggatgagt ttaagagcgg ctctttgggc   1080

cgtgtcactc cactcatggc tgctaagatc gctgatcgcg aaactggtaa ggctttgggc   1140

ccgaaccaag tgggcgagct gtgtatcaaa ggccctatgg tgagcaaggg ttatgtcaat   1200

aacgttgaag ctaccaagga ggccatcgac gacgacggct ggttgcattc tggtgatttt   1260

ggatattacg acgaagatga gcatttttac gtcgtggatc gttacaagga gctgatcaaa   1320

tacaagggtc gccaggttgc tccagctgag ttggaggaga ttctgttgaa aaatccatgc   1380

attcgcgatg tcgctgtggt cggcattcct gatctggagg ccggcgaact gccttctgct   1440

ttcgttgtca agcagcctgg tacagaaatt accgccaaag aagtgtatga ttacctggct   1500

gaacgtgtga gccatactaa gtacttgcgt ggcggcgtgc gttttgttga ctccatccct   1560

cgtaacgtaa caggcaaaat tacccgcaag gagctgttga aacaattgtt ggtgaaggcc   1620

ggcggtgttt aa                                                       1632


