                         SEQUENCE LISTING

<110>  Stellenbosch University
 
<120>  A MODIFIED BETA-FRUCTOFURANOSIDASE FOR FRUCTOOLIGOSACCHARIDE 
       PRODUCTION

<130>  P2807PC00

<150>  ZA 2014/07138
<151>  2014-10-02

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  2007
<212>  DNA
<213>  Aspergillus japonicus

<400>  1
atggtcagct tcactagtct gttggctgga gttgcagcta tctccggtgt gctggcagct       60

cctgctgccg aagtagagtc tgtagcagtg gaaaagagat cttaccacct agatacaaca      120

gcccctcctc caacaaattt gagtacactt ccaaacaata cactgttcca tgtttggagg      180

ccaagagcac atattttgcc agccgaaggt caaatcggtg atccctgtgc acactacact      240

gacccatcaa ctggtctgtt tcatgttgga tttttgcacg acggtgatgg aattgcagga      300

gccaccaccg ctaatttggc tacttatacg gacacgtccg acaacggttc atttttgatt      360

caacccggtg gcaaaaacga tcctgtcgct gtgtttgacg gtgcagttat acctgtcggt      420

gtgaataata ctccaacgtt gctgtatact tctgttagct ttttgccaat acattggtcc      480

atcccgtaca ctcgtggaag cgagactcaa tcattggctg ttgccagaga tggtggtaga      540

agattcgata aactggatca aggtcccgta atcgcagatc acccatttgc agtggatgtg      600

accgccttca gagatccttt tgttttccgt tcggctaagc tagatgtcct gttatctctt      660

gacgaggaag tagctaggaa tgaaaccgca gtgcaacagg ctgttgacgg atggactgag      720

aaaaatgctc cttggtacgt ggccgtatcc ggaggagttc atggagtcgg acccgcacaa      780

ttcctataca gacaaaatgg tggaaatgca agcgagttcc aatactggga gtatttggga      840

gaatggtggc aggaagctac taatagttca tggggtgacg agggaacatg ggctggcaga      900

tggggtttca atttcgaaac aggaaatgtg ttatttctaa ctgaggaagg ccacgatcct      960

caaacaggcg aggtgttcgt tactttgggt acagagggat caggtttgcc aattgttccc     1020

caagtgagta gcattcatga catgttgtgg gcagctggtg aagttggtgt tggttccgag     1080

caggaaggcg ccaaagtgga gttctcccct tccatggctg gtttccttga ctggggcttt     1140

tctgcatacg cagccgctgg taaagttttg cctgcctcat ccgcagtttc taagacatct     1200

ggtgttgaag ttgacagata tgtctccttc gtttggttga ctggtgacca gtatgagcaa     1260

gctgacggtt ttccaaccgc tcagcaagga tggacaggtt ccttgttatt gccacgtgag     1320

cttaaggtcc aaactgttga aaacgtggtt gacaacgaac tggtgcgtga agagggtgtt     1380

tcctgggtcg ttggtgagag tgataaccag actgccaggt tacgaacttt aggtatcact     1440

atcgctagag aaacaaaagc tgcattgttg gcaaacggtt ccgtgacagc tgaggaggat     1500

agaaccctac aaaccgctgc tgttgttcca ttcgctcagt caccatcgtc caagtttttc     1560

gttttgacgg ctcagcttga atttcctgct tctgctagat catcacctct gcaatccggt     1620

tttgaaattc tcgcttcgga attggagaga accgctatct actaccaatt ttctaacgaa     1680

agcctagtgg ttgaccgttc tcagactagc gcagcagctc caactaatcc tggtcttgat     1740

tcgttcacag aatctggtaa gctgagactt ttcgatgtca tcgaaaatgg tcaagagcaa     1800

gtcgagacat tagacttgac ggtagtggta gacaacgctg tggtcgaagt ctatgctaat     1860

ggaagatttg ctttgtctac ttgggccagg tcatggtatg ataattccac acaaattcgt     1920

ttctttcata acggagaggg agaggtacag tttagaaatg ttagcgttag tgaaggtctg     1980

tacaatgcat ggccagaacg taactaa                                         2007


<210>  2
<211>  2007
<212>  DNA
<213>  Aspergillus japonicus

<400>  2
atggtttctt tcacatcctt gttggctggt gttgctgcta tttccggtgt tttggctgct       60

ccagctgctg aagttgaatc cgttgctgtt gagaagagat cctaccactt ggatactact      120

gctccaccac caactaactt gtccactttg ccaaacaaca ctttgttcca tgtttggaga      180

ccaagagcac acattttgcc agctgagggt caaattggtg atccatgtgc tcactacact      240

gacccatcca ctggtttgtt tcatgttggt ttcttgcacg acggtgatgg tattgctggt      300

gctactactg ctaacttggc tacttacact gacacttccg acaacggttc cttcttgatt      360

caacctggtg gaaagaacga tccagttgct gttttcgacg gtgctgttat cccagttggt      420

gttaacaaca ctccaacttt gttgtacact tccgtttctt tcttgccaat ccactggtcc      480

attccataca ctagaggttc cgagactcaa tctttggctg ttgctagaga tggtggtaga      540

agattcgaca agttggacca gggtccagtt attgctgatc acccattcgc tgttgacgtt      600

actgctttca gagatccatt cgttttcaga tccgctaagt tggacgtttt gttgtccttg      660

gacgaggaag ttgctagaaa cgagactgct gttcaacaag ctgttgacgg atggactgaa      720

aagaacgctc cttggtacgt tgctgtttct ggtggtgttc atggtgttgg tccagctcag      780

ttcttgtaca gacagaacgg tggtaacgct tctgagttcc agtactggga atacttggga      840

gaatggtggc aagaagctac taattcttcc tggggtgatg agggaacttg ggctggtaga      900

tggggtttca acttcgagac tggtaacgtt ttgttcttga ctgaagaggg tcacgatcca      960

caaactggtg aagttttcgt tactttgggt actgaaggtt ccggattgcc aattgttcct     1020

caggtttcct ccattcacga tatgttgtgg gctgctggtg aagttggtgt tggttctgaa     1080

caagagggtg ctaaggttga gttctctcca tctatggctg gtttcttgga ctggggattc     1140

tctgcttatg ctgctgctgg aaaggttttg ccagcttctt ctgctgtttc caagacttcc     1200

ggtgttgagg ttgacagata cgtttccttt gtttggttga ctggtgacca atacgaacaa     1260

gctgacggtt ttccaactgc tcaacaggga tggactggtt ctttgttgtt gccaagagag     1320

ttgaaggttc agactgttga gaacgttgtt gacaacgagc ttgttagaga agagggagtt     1380

tcctgggttg tcggtgaatc cgacaatcag actgctagat tgagaacttt gggtatcact     1440

atcgctagag agactaaggc tgctttgttg gctaacggtt ccgttactgc tgaagaggac     1500

agaactttgc agactgctgc tgttgttcca ttcgctcaat ctccatcctc caagttcttc     1560

gttttgactg ctcagttgga gtttccagct tctgctagat cctctccatt gcaatccggt     1620

ttcgagattt tggcttccga gttggagaga actgctatct actaccagtt ctccaacgag     1680

tctttggttg ttgacagatc ccaaacttct gctgctgctc caactaaccc aggattggac     1740

tctttcactg agtccggtaa gttgagattg ttcgacgtta tcgagaacgg tcaagagcaa     1800

gttgagactt tggacttgac tgttgttgtt gataacgctg ttgttgaggt ttacgctaac     1860

ggtagattcg ctttgtctac ttgggctaga tcctggtacg acaactccac tcagatcaga     1920

ttcttccaca acggtgaagg tgaagttcag ttcagaaacg tttccgtttc cgagggtttg     1980

tacaacgctt ggccagagag aaactaa                                         2007


<210>  3
<211>  635
<212>  PRT
<213>  Aspergillus japonicus

<400>  3

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  4
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-A159P-G302N-Q471S 
       substitutions

<400>  4

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  5
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-G302N-Q471S 
       substitutions

<400>  5

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  6
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-F121Y-A159P 
       substitutions

<400>  6

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  7
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-Q471S-A159P 
       substitutions

<400>  7

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  8
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-Q471S-A159P 
       substitutions

<400>  8

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  9
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-F121Y substitutions

<400>  9

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  10
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-Q471S substitutions

<400>  10

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  11
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-A159P substitutions

<400>  11

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  12
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with (F121Y-Q471S 
       substitutions

<400>  12

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  13
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-A159P substitutions

<400>  13

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  14
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with Q471S-A159P substitutions

<400>  14

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Pro Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  15
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-F121Y-Q471S-A159S 
       substitutions

<400>  15

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  16
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-F121Y-A159S 
       substitutions

<400>  16

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  17
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-Q471S-A159S 
       substitutions

<400>  17

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  18
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-Q471S-A159S 
       substitutions

<400>  18

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  19
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with G302N-A159S substitutions

<400>  19

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Asn Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  20
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with F121Y-A159S substitutions

<400>  20

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Tyr Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  21
<211>  635
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of FopA from A. japonicus, with Q471S-A159S substitutions

<400>  21

Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser Thr 
1               5                   10                  15      


Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His Ile 
            20                  25                  30          


Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr Asp 
        35                  40                  45              


Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp Gly 
    50                  55                  60                  


Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr Ser 
65                  70                  75                  80  


Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro Val 
                85                  90                  95      


Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr Pro 
            100                 105                 110         


Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser Ile 
        115                 120                 125             


Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg Asp 
    130                 135                 140                 


Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ser Asp 
145                 150                 155                 160 


His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val Phe 
                165                 170                 175     


Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val Ala 
            180                 185                 190         


Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu Lys 
        195                 200                 205             


Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val Gly 
    210                 215                 220                 


Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu Phe 
225                 230                 235                 240 


Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn Ser 
                245                 250                 255     


Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn Phe 
            260                 265                 270         


Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro Gln 
        275                 280                 285             


Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu Pro 
    290                 295                 300                 


Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala Gly 
305                 310                 315                 320 


Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe Ser 
                325                 330                 335     


Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala Ala 
            340                 345                 350         


Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser Gly 
        355                 360                 365             


Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp Gln 
    370                 375                 380                 


Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr Gly 
385                 390                 395                 400 


Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn Val 
                405                 410                 415     


Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val Gly 
            420                 425                 430         


Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr Ile 
        435                 440                 445             


Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr Ala 
    450                 455                 460                 


Glu Glu Asp Arg Thr Leu Ser Thr Ala Ala Val Val Pro Phe Ala Gln 
465                 470                 475                 480 


Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe Pro 
                485                 490                 495     


Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu Ala 
            500                 505                 510         


Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu Ser 
        515                 520                 525             


Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn Pro 
    530                 535                 540                 


Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp Val 
545                 550                 555                 560 


Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val Val 
                565                 570                 575     


Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala Leu 
            580                 585                 590         


Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg Phe 
        595                 600                 605             


Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val Ser 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
625                 630                 635 


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  22
gtttagtaga acctcgtgaa actta                                             25


<210>  23
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  23
acttaaaata cgctgaaccc gaacat                                            26


<210>  24
<211>  150
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artifical construct encoding the xyn2 secretion signal, 6 
       histidine residues and a factor Xa protease cleavage site

<400>  24
atggtttctt tcacatcctt gttggctggt gttgctgcta tttccggtgt tttggctgct       60

ccagctgctg aagttgaatc cgttgctgtt gagaagagac atcaccatca ccatcacgga      120

tccggctctg gatctggtat cgagggaaga                                       150


<210>  25
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  xyn2 secretion signal

<400>  25

Met Val Ser Phe Thr Ser Leu Leu Ala Gly Val Ala Ala Ile Ser Gly 
1               5                   10                  15      


Val Leu Ala Ala Pro Ala Ala Glu Val Glu Ser Val Ala Val Glu Lys 
            20                  25                  30          


Arg 
    


<210>  26
<211>  668
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  xyn2 secretion signal and FopA polypeptide

<400>  26

Met Val Ser Phe Thr Ser Leu Leu Ala Gly Val Ala Ala Ile Ser Gly 
1               5                   10                  15      


Val Leu Ala Ala Pro Ala Ala Glu Val Glu Ser Val Ala Val Glu Lys 
            20                  25                  30          


Arg Ser Tyr His Leu Asp Thr Thr Ala Pro Pro Pro Thr Asn Leu Ser 
        35                  40                  45              


Thr Leu Pro Asn Asn Thr Leu Phe His Val Trp Arg Pro Arg Ala His 
    50                  55                  60                  


Ile Leu Pro Ala Glu Gly Gln Ile Gly Asp Pro Cys Ala His Tyr Thr 
65                  70                  75                  80  


Asp Pro Ser Thr Gly Leu Phe His Val Gly Phe Leu His Asp Gly Asp 
                85                  90                  95      


Gly Ile Ala Gly Ala Thr Thr Ala Asn Leu Ala Thr Tyr Thr Asp Thr 
            100                 105                 110         


Ser Asp Asn Gly Ser Phe Leu Ile Gln Pro Gly Gly Lys Asn Asp Pro 
        115                 120                 125             


Val Ala Val Phe Asp Gly Ala Val Ile Pro Val Gly Val Asn Asn Thr 
    130                 135                 140                 


Pro Thr Leu Leu Tyr Thr Ser Val Ser Phe Leu Pro Ile His Trp Ser 
145                 150                 155                 160 


Ile Pro Tyr Thr Arg Gly Ser Glu Thr Gln Ser Leu Ala Val Ala Arg 
                165                 170                 175     


Asp Gly Gly Arg Arg Phe Asp Lys Leu Asp Gln Gly Pro Val Ile Ala 
            180                 185                 190         


Asp His Pro Phe Ala Val Asp Val Thr Ala Phe Arg Asp Pro Phe Val 
        195                 200                 205             


Phe Arg Ser Ala Lys Leu Asp Val Leu Leu Ser Leu Asp Glu Glu Val 
    210                 215                 220                 


Ala Arg Asn Glu Thr Ala Val Gln Gln Ala Val Asp Gly Trp Thr Glu 
225                 230                 235                 240 


Lys Asn Ala Pro Trp Tyr Val Ala Val Ser Gly Gly Val His Gly Val 
                245                 250                 255     


Gly Pro Ala Gln Phe Leu Tyr Arg Gln Asn Gly Gly Asn Ala Ser Glu 
            260                 265                 270         


Phe Gln Tyr Trp Glu Tyr Leu Gly Glu Trp Trp Gln Glu Ala Thr Asn 
        275                 280                 285             


Ser Ser Trp Gly Asp Glu Gly Thr Trp Ala Gly Arg Trp Gly Phe Asn 
    290                 295                 300                 


Phe Glu Thr Gly Asn Val Leu Phe Leu Thr Glu Glu Gly His Asp Pro 
305                 310                 315                 320 


Gln Thr Gly Glu Val Phe Val Thr Leu Gly Thr Glu Gly Ser Gly Leu 
                325                 330                 335     


Pro Ile Val Pro Gln Val Ser Ser Ile His Asp Met Leu Trp Ala Ala 
            340                 345                 350         


Gly Glu Val Gly Val Gly Ser Glu Gln Glu Gly Ala Lys Val Glu Phe 
        355                 360                 365             


Ser Pro Ser Met Ala Gly Phe Leu Asp Trp Gly Phe Ser Ala Tyr Ala 
    370                 375                 380                 


Ala Ala Gly Lys Val Leu Pro Ala Ser Ser Ala Val Ser Lys Thr Ser 
385                 390                 395                 400 


Gly Val Glu Val Asp Arg Tyr Val Ser Phe Val Trp Leu Thr Gly Asp 
                405                 410                 415     


Gln Tyr Glu Gln Ala Asp Gly Phe Pro Thr Ala Gln Gln Gly Trp Thr 
            420                 425                 430         


Gly Ser Leu Leu Leu Pro Arg Glu Leu Lys Val Gln Thr Val Glu Asn 
        435                 440                 445             


Val Val Asp Asn Glu Leu Val Arg Glu Glu Gly Val Ser Trp Val Val 
    450                 455                 460                 


Gly Glu Ser Asp Asn Gln Thr Ala Arg Leu Arg Thr Leu Gly Ile Thr 
465                 470                 475                 480 


Ile Ala Arg Glu Thr Lys Ala Ala Leu Leu Ala Asn Gly Ser Val Thr 
                485                 490                 495     


Ala Glu Glu Asp Arg Thr Leu Gln Thr Ala Ala Val Val Pro Phe Ala 
            500                 505                 510         


Gln Ser Pro Ser Ser Lys Phe Phe Val Leu Thr Ala Gln Leu Glu Phe 
        515                 520                 525             


Pro Ala Ser Ala Arg Ser Ser Pro Leu Gln Ser Gly Phe Glu Ile Leu 
    530                 535                 540                 


Ala Ser Glu Leu Glu Arg Thr Ala Ile Tyr Tyr Gln Phe Ser Asn Glu 
545                 550                 555                 560 


Ser Leu Val Val Asp Arg Ser Gln Thr Ser Ala Ala Ala Pro Thr Asn 
                565                 570                 575     


Pro Gly Leu Asp Ser Phe Thr Glu Ser Gly Lys Leu Arg Leu Phe Asp 
            580                 585                 590         


Val Ile Glu Asn Gly Gln Glu Gln Val Glu Thr Leu Asp Leu Thr Val 
        595                 600                 605             


Val Val Asp Asn Ala Val Val Glu Val Tyr Ala Asn Gly Arg Phe Ala 
    610                 615                 620                 


Leu Ser Thr Trp Ala Arg Ser Trp Tyr Asp Asn Ser Thr Gln Ile Arg 
625                 630                 635                 640 


Phe Phe His Asn Gly Glu Gly Glu Val Gln Phe Arg Asn Val Ser Val 
                645                 650                 655     


Ser Glu Gly Leu Tyr Asn Ala Trp Pro Glu Arg Asn 
            660                 665             


