                         SEQUENCE LISTING

<110>  RESEARCH DEVELOPMENT FOUNDATION
 
<120>  METHODS FOR GENERATING ENGINEERED ENZYMES

<130>  CLFR.P0415WO

<140>  UNKNOWN
<141>  2015-10-14

<150>  US 62/063,836
<151>  2014-10-14

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  1

Glu Asn Leu Tyr Phe Glu Ser 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  2

Glu Asn Leu Tyr Phe His Ser 
1               5           


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  3

Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      


Leu Ala 
        


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  4

Phe Glu His Asp Glu Leu 
1               5       


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  5

Lys Asp Glu Leu 
1               


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  6

His Asp Glu Leu 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  7

Arg Asp Glu Leu 
1               


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  8

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  9

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Xaa can be any naturally occurring amino acid

<400>  10

Glu Asn Leu Tyr Phe Xaa Ser 
1               5           


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  11

His His His His His His 
1               5       


<210>  12
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  12

Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val Leu 
1               5                   10                  15      


Ala 
    


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  13

Leu Pro Thr Glu Gly 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  14

Val Ala Arg Arg Asp 
1               5   


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  15

Leu Pro Glu Thr Gly 
1               5   


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  16

Lys Glu Glu Leu 
1               


<210>  17
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  17

Lys Lys Xaa Xaa 
1               


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  18

Val Glu Lys Pro Phe Ala Ile Ala Lys Glu 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  19

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  20

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  21

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  22
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  22

Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
1               5                   10      


<210>  23
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  23

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  24

Xaa Asn Leu Xaa Phe Xaa Xaa 
1               5           


<210>  25
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  25
ctagtatcga tgcagttact tcgctgtttc tcaatctttt cggtgattgc tagcgtttta       60

gcacaggaac tgacaactat atgcg                                             85


<210>  26
<211>  179
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (130)..(131)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (133)..(134)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (136)..(137)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (139)..(140)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (142)..(143)
<223>  n is a, c, g, or t

<400>  26
ggagacttga ccaaacctct ggcgaagaat tgttaattaa gagcgcatgc cgactcctgc       60

agtcacaatt cgtcgtgttc gaaactacca gcgtagtctg gaacgtcgta tgggtaacta      120

ccactgccsn nsnnsnnsnn snnactacca ctgcctttat cgtcgtcatc tttataatc       179


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  27
gcgtagtctg gaacgtcgta tggg                                              24


<210>  28
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (124)..(125)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (127)..(128)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (133)..(134)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (142)..(143)
<223>  n is a, c, g, or t

<400>  28
ggagacttga ccaaacctct ggcgaagaat tgttaattaa gagcgcatgc cgactcctgc       60

agtcacaatt cgtcgtgttc gaaactacca gcgtagtctg gaacgtcgta tgggtaactg      120

ccsnnsnnga asnncaaatt snnactacct ttatcgtcgt catctttata atc             173


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  29

Leu Pro Glu Thr Ala 
1               5   


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Ala or Gly

<400>  30

Leu Pro Glu Thr Xaa 
1               5   


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  31

Arg Leu Thr Pro Arg 
1               5   


<210>  32
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  32

Ser Pro Ala Lys Arg 
1               5   


<210>  33
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  33

Ala Arg Lys Pro Ala 
1               5   


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  34

Gly Ser Phe Arg Pro 
1               5   


<210>  35
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  35

Asn Ala Phe Ser His 
1               5   


<210>  36
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  36

Ala Leu Ala Arg Arg 
1               5   


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  37

Leu Arg Pro Arg Ala 
1               5   


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  38

Ala Leu Ser Arg Arg 
1               5   


<210>  39
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  39

Arg Leu Arg Pro Arg 
1               5   


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  40

Arg Leu Leu Pro Arg 
1               5   


<210>  41
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  41

Arg Leu Ser Arg Arg 
1               5   


<210>  42
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  42

Pro Leu Leu Pro Arg 
1               5   


<210>  43
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  43

Pro Leu Leu Arg Arg 
1               5   


<210>  44
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  44

Pro Leu Arg Pro Arg 
1               5   


<210>  45
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  45

Arg Leu Ala Pro Arg 
1               5   


<210>  46
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  46

Ala Leu Leu Pro Arg 
1               5   


<210>  47
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  47

Pro Leu Leu Ala Arg 
1               5   


<210>  48
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  48

Pro Leu Val Pro Arg 
1               5   


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  49

Ser Leu Arg Arg Arg 
1               5   


<210>  50
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  50

Leu Leu Cys Lys Arg 
1               5   


<210>  51
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  51

Leu Gln Pro Arg Ala 
1               5   


<210>  52
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  52

Tyr Pro Val Cys Val 
1               5   


<210>  53
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  53

Ser Pro Ala Trp Arg 
1               5   


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  54

Leu Xaa Xaa Arg 
1               


<210>  55
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Xaa is Leu, Phe, Met, Ile, or Val

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  55

Xaa Xaa Xaa Arg 
1               


