                         SEQUENCE LISTING

<110>  DANISCO US INC.
 
<120>  ENHANCED PROTEIN EXPRESSION

<130>  40772-WO-PCT

<150>  62/092461
<151>  2014-12-16

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  336
<212>  DNA
<213>  Unknown

<220>
<223>  Bacillus sp.

<400>  1
ttgattggcc agcgtattaa acaataccgt aaagaaaaag gctactcact atcagaacta       60

gctgaaaaag ctggggtagc gaagtcttat ttaagctcaa tagaacgaaa tctacaaacg      120

aacccctcca ttcaatttct cgaaaaagtc tccgctgttc tggacgtctc ggttcatact      180

ttgctcgatg agaaacatga aaccgaatac gatggtcaat tagatagtga atgggagaaa      240

ttggttcgcg atgcgatgac atccggggta tcgaaaaaac aatttcgtga atttttagat      300

tatcaaaaat ggagaaaatc ccaaaaagag gagtag                                336


<210>  2
<211>  111
<212>  PRT
<213>  Unknown

<220>
<223>  Bacillus sp.

<400>  2

Met Ile Gly Gln Arg Ile Lys Gln Tyr Arg Lys Glu Lys Gly Tyr Ser 
1               5                   10                  15      


Leu Ser Glu Leu Ala Glu Lys Ala Gly Val Ala Lys Ser Tyr Leu Ser 
            20                  25                  30          


Ser Ile Glu Arg Asn Leu Gln Thr Asn Pro Ser Ile Gln Phe Leu Glu 
        35                  40                  45              


Lys Val Ser Ala Val Leu Asp Val Ser Val His Thr Leu Leu Asp Glu 
    50                  55                  60                  


Lys His Glu Thr Glu Tyr Asp Gly Gln Leu Asp Ser Glu Trp Glu Lys 
65                  70                  75                  80  


Leu Val Arg Asp Ala Met Thr Ser Gly Val Ser Lys Lys Gln Phe Arg 
                85                  90                  95      


Glu Phe Leu Asp Tyr Gln Lys Trp Arg Lys Ser Gln Lys Glu Glu 
            100                 105                 110     


<210>  3
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  3
ttgattggcc agcgtattaa acaataccgt aaagaaaaag gctactcact atcagaacta       60

gctgaaaaag ctggggtagc gaagtcttat ttaagctcaa tagaacgaaa tctacaaacg      120

aacccctcca ttcaatttct cgaaaaagtc tccgctgttc tggacgtctc ggttcatact      180

ttgctcgatg agaaacatga aaccgaatac gatggtcaat tagatagtga atgggagaaa      240

ttggttcgcg atgcgatgac atccggggta tcgaaaaaac aatttcgtga atttttagat      300

tatcaaaaat ggagaaaatc ccaaaaagaa gagtag                                336


<210>  4
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward Primer

<400>  4
caggaattcc tgacgtctca aatatgtgac tattg                                  35


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse Primer

<400>  5
ctcggatccg agaaattgaa agaaagacaa aagc                                   34


<210>  6
<211>  275
<212>  PRT
<213>  Unknown

<220>
<223>  Bacillus sp.

<400>  6

Ala Gln Ser Val Pro Tyr Gly Val Ser Gln Ile Lys Ala Pro Ala Leu 
1               5                   10                  15      


His Ser Gln Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val Ile Asp 
            20                  25                  30          


Ser Gly Ile Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 
        35                  40                  45              


Ser Met Val Pro Ser Glu Thr Asn Pro Phe Gln Asp Asn Asn Ser His 
    50                  55                  60                  


Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser Ile Gly 
65                  70                  75                  80  


Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 
                85                  90                  95      


Gly Ala Asp Gly Ser Gly Gln Tyr Ser Trp Ile Ile Asn Gly Ile Glu 
            100                 105                 110         


Trp Ala Ile Ala Asn Asn Met Asp Val Ile Asn Met Ser Leu Gly Gly 
        115                 120                 125             


Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala 
    130                 135                 140                 


Ser Gly Val Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 
145                 150                 155                 160 


Ser Ser Ser Thr Val Gly Tyr Pro Gly Lys Tyr Pro Ser Val Ile Ala 
                165                 170                 175     


Val Gly Ala Val Asp Ser Ser Asn Gln Arg Ala Ser Phe Ser Ser Val 
            180                 185                 190         


Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser Ile Gln Ser Thr 
        195                 200                 205             


Leu Pro Gly Asn Lys Tyr Gly Ala Leu Asn Gly Thr Ser Met Ala Ser 
    210                 215                 220                 


Pro His Val Ala Gly Ala Ala Ala Leu Ile Leu Ser Lys His Pro Asn 
225                 230                 235                 240 


Trp Thr Asn Thr Gln Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 
                245                 250                 255     


Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu Ile Asn Val Gln Ala 
            260                 265                 270         


Ala Ala Gln 
        275 


<210>  7
<211>  425
<212>  PRT
<213>  Unknown

<220>
<223>  Bacillus sp.

<400>  7

Leu Thr Ala Pro Ser Ile Lys Ser Gly Thr Ile Leu His Ala Trp Asn 
1               5                   10                  15      


Trp Ser Phe Asn Thr Leu Lys His Asn Met Lys Asp Ile His Asp Ala 
            20                  25                  30          


Gly Tyr Thr Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys Glu Gly 
        35                  40                  45              


Asn Gln Gly Asp Lys Ser Met Ser Asn Trp Tyr Trp Leu Tyr Gln Pro 
    50                  55                  60                  


Thr Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln Glu Phe 
65                  70                  75                  80  


Lys Glu Met Cys Ala Ala Ala Glu Glu Tyr Gly Ile Lys Val Ile Val 
                85                  90                  95      


Asp Ala Val Ile Asn His Thr Thr Ser Asp Tyr Ala Ala Ile Ser Asn 
            100                 105                 110         


Glu Val Lys Ser Ile Pro Asn Trp Thr His Gly Asn Thr Gln Ile Lys 
        115                 120                 125             


Asn Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu Gly Leu 
    130                 135                 140                 


Tyr Asp Trp Asn Thr Gln Asn Thr Gln Val Gln Ser Tyr Leu Lys Arg 
145                 150                 155                 160 


Phe Leu Asp Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg Phe Asp 
                165                 170                 175     


Ala Ala Lys His Ile Glu Leu Pro Asp Asp Gly Ser Tyr Gly Ser Gln 
            180                 185                 190         


Phe Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr Gly Glu 
        195                 200                 205             


Ile Leu Gln Asp Ser Ala Ser Arg Asp Ala Ala Tyr Ala Asn Tyr Met 
    210                 215                 220                 


Asp Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala Leu Lys 
225                 230                 235                 240 


Asn Arg Asn Leu Gly Val Ser Asn Ile Ser His Tyr Ala Ser Asp Val 
                245                 250                 255     


Ser Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr Tyr Ala 
            260                 265                 270         


Asn Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile Arg Leu 
        275                 280                 285             


Gly Trp Ala Val Ile Ala Ser Arg Ser Gly Ser Thr Pro Leu Phe Phe 
    290                 295                 300                 


Ser Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly Lys Ser 
305                 310                 315                 320 


Gln Ile Gly Asp Arg Gly Ser Ala Leu Phe Glu Asp Gln Ala Ile Thr 
                325                 330                 335     


Ala Val Asn Arg Phe His Asn Val Met Ala Gly Gln Pro Glu Glu Leu 
            340                 345                 350         


Ser Asn Pro Asn Gly Asn Asn Gln Ile Phe Met Asn Gln Arg Gly Ser 
        355                 360                 365             


His Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Ser Ile Asn 
    370                 375                 380                 


Thr Ala Thr Lys Leu Pro Asp Gly Arg Tyr Asp Asn Lys Ala Gly Ala 
385                 390                 395                 400 


Gly Ser Phe Gln Val Asn Asp Gly Lys Leu Thr Gly Thr Ile Asn Ala 
                405                 410                 415     


Arg Ser Val Ala Val Leu Tyr Pro Asp 
            420                 425 


<210>  8
<211>  221
<212>  PRT
<213>  Unknown

<220>
<223>  Bacillus sp.

<400>  8

Met Lys Lys Ile Val Ser Ile Leu Phe Met Phe Gly Leu Val Met Gly 
1               5                   10                  15      


Phe Ser Gln Phe Gln Pro Ser Thr Val Phe Ala Ala Asp Lys Val Val 
            20                  25                  30          


His Glu Thr Ile Ile Val Pro Lys Asn Thr Thr Tyr Asp Gly Lys Gly 
        35                  40                  45              


Gln Arg Phe Val Ala Gly Lys Glu Leu Gly Asp Gly Ser Gln Ser Glu 
    50                  55                  60                  


Asn Gln Asp Pro Val Phe Arg Val Glu Asp Gly Ala Thr Leu Lys Asn 
65                  70                  75                  80  


Val Val Leu Gly Ala Pro Ala Ala Asp Gly Val His Thr Tyr Gly Asn 
                85                  90                  95      


Val Asn Ile Gln Asn Val Lys Trp Glu Asp Val Gly Glu Asp Ala Leu 
            100                 105                 110         


Thr Val Lys Lys Glu Gly Lys Val Thr Ile Asp Gly Gly Ser Ala Gln 
        115                 120                 125             


Lys Ala Ser Asp Lys Ile Phe Gln Ile Asn Lys Ala Ser Thr Phe Thr 
    130                 135                 140                 


Val Lys Asn Phe Thr Ala Asp Asn Gly Gly Lys Phe Ile Arg Gln Leu 
145                 150                 155                 160 


Gly Gly Ser Thr Phe His Val Asp Val Ile Ile Asp Lys Cys Thr Ile 
                165                 170                 175     


Thr Asn Met Lys Glu Ala Ile Phe Arg Thr Asp Ser Lys Thr Ser Thr 
            180                 185                 190         


Val Arg Met Thr Asn Thr Arg Tyr Ser Asn Val Gly Gln Lys Trp Ile 
        195                 200                 205             


Gly Val Gln His Ile Tyr Glu Asn Asn Asn Thr Gln Phe 
    210                 215                 220     


