                         SEQUENCE LISTING

<110>  PROMEGA CORPORATION
 
<120>  LUCIFERASE-BASED THERMAL SHIFT ASSAYS

<130>  PRMG-34095/WO-1/ORD

<150>  US 62/112,518
<151>  2015-02-05

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  170
<212>  PRT
<213>  Oplophorus gracilirostris

<400>  1

Met Phe Thr Leu Ala Asp Phe Val Gly Asp Trp Gln Gln Thr Ala Gly 
1               5                   10                  15      


Tyr Asn Gln Asp Gln Val Leu Glu Gln Gly Gly Leu Ser Ser Leu Phe 
            20                  25                  30          


Gln Ala Leu Gly Val Ser Val Thr Pro Ile Gln Lys Val Val Leu Ser 
        35                  40                  45              


Gly Glu Asn Gly Leu Lys Ala Asp Ile His Val Ile Ile Pro Tyr Glu 
    50                  55                  60                  


Gly Leu Ser Gly Phe Gln Met Gly Leu Ile Glu Met Ile Phe Lys Val 
65                  70                  75                  80  


Val Tyr Pro Val Asp Asp His His Phe Lys Ile Ile Leu His Tyr Gly 
                85                  90                  95      


Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe Gly 
            100                 105                 110         


Arg Pro Tyr Pro Gly Ile Ala Val Phe Asp Gly Lys Gln Ile Thr Val 
        115                 120                 125             


Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Tyr Asp Glu Arg Leu Ile 
    130                 135                 140                 


Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val Thr 
145                 150                 155                 160 


Gly Trp Arg Leu Cys Glu Asn Ile Leu Ala 
                165                 170 


<210>  2
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  2

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn Gly Val 
145                 150                 155                 160 


Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
                165                 170     


<210>  3
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  3

Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
1               5                   10              


<210>  4
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  4

Met Val Phe Thr Leu Ala Asp Phe Val Gly Asp Trp Gln Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Gln Asp Gln Val Leu Glu Gln Gly Gly Leu Ser Ser Leu 
            20                  25                  30          


Phe Gln Ala Leu Gly Val Ser Val Thr Pro Ile Gln Lys Val Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Gly Leu Lys Ala Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Phe Gln Met Gly Leu Ile Glu Met Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Ile Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Pro Gly Ile Ala Val Phe Asp Gly Lys Gln Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Tyr Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
145                 150                 155             


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  5

Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser 
1               5                   10      


<210>  6
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  6

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr Ala 
1               5                   10                  15      


Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val Arg 
        35                  40                  45              


Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
145                 150                 155                 


<210>  7
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic enzyeme

<400>  7

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Arg Gln Thr Ala 
1               5                   10                  15      


Gly Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Phe Gln Asn Leu Gly Val Ser Val Thr Pro Ile Gln Arg Ile Val Leu 
        35                  40                  45              


Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Gly Asp Gln Met Gly Gln Ile Glu Lys Ile Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu His Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Ile Asp Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Asn Pro Asp Gly Ser Leu Leu Phe Arg Val Thr Ile Asn 
145                 150                 155             


