                         SEQUENCE LISTING

<110>  Purac Biochem B.V.
 
<120>  Dehydrogenase-catalysed production of FDCA

<130>  P6054479PCT

<150>  EP15155401
<151>  2015-02-17

<160>  40    

<170>  PatentIn version 3.3

<210>  1
<211>  392
<212>  PRT
<213>  Aeribacillus pallidus

<400>  1

Met Ile Gly Asn Tyr Ala Lys Lys Ala Ile Asp Phe Glu Phe Thr Phe 
1               5                   10                  15      


Tyr Leu Pro Thr Leu Ile Glu Phe Gly Tyr Gly Lys Ala Ser Arg Met 
            20                  25                  30          


Gly Glu Met Leu Glu Gln Met Gly Ile Lys Asn Val Phe Leu Val Thr 
        35                  40                  45              


Asp Lys Gly Val Glu Ala Ala Gly Leu Leu Ala Gly Ile Val Gln Ser 
    50                  55                  60                  


Leu Glu Ser Ser Asn Ile Arg Tyr Val Ile Tyr Ser Asp Val Glu Pro 
65                  70                  75                  80  


Asp Pro Ser Leu Glu Thr Ile Asp Arg Gly Ala Ser Val Phe Lys Glu 
                85                  90                  95      


Gln Ser Phe Asp Cys Ile Leu Ala Val Gly Gly Gly Ser Pro Ile Asp 
            100                 105                 110         


Thr Ala Lys Gly Ile Arg Val Val Val Thr Asn Gly Gly Asn Ile Gly 
        115                 120                 125             


Asp Tyr Ala Gly Val Asn Arg Val Ala Lys Lys Ser Glu Ile Pro Leu 
    130                 135                 140                 


Val Ala Val Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Ile Phe 
145                 150                 155                 160 


Gly Val Tyr Ser Asp Trp Glu Asn Gln Val Lys Val Thr Val Thr Ser 
                165                 170                 175     


Pro Tyr Met Ala Pro Glu Ile Ala Leu Val Asp Pro Glu Leu Thr Met 
            180                 185                 190         


Ser Leu Pro Gln Lys Met Thr Ala Ala Ser Gly Ile Asp Ala Leu Ala 
        195                 200                 205             


His Gly Ile Glu Thr Phe Phe Ser Leu Arg Ser Arg Pro Ala Ser Asp 
    210                 215                 220                 


Ala Leu Ala Val Glu Ala Met Ala Thr Val Ser Ala Tyr Leu Arg Arg 
225                 230                 235                 240 


Ala Val Glu Asp Gly Thr Asp Lys Glu Ala Arg Ile Gly Met Ser Gln 
                245                 250                 255     


Gly Ser Leu Leu Ala Gly Met Ala Phe Asn Asn Gly Phe Leu Gly Leu 
            260                 265                 270         


Ala His Ala Ile Gly Ser Ala Leu Ser Gly His Cys His Val Ser His 
        275                 280                 285             


Gly Val Ala Ile Gly Leu Leu Leu Pro Lys Val Val Glu Phe Asn Ala 
    290                 295                 300                 


Arg Val Arg Pro Glu Lys Ala Ala Lys Ile Ala Glu Leu Leu Gly Val 
305                 310                 315                 320 


Lys Gly Asp Arg Glu Glu Val Leu Ala Glu Gln Ala Ala Pro Ala Val 
                325                 330                 335     


Ala Ser Leu Val Lys Glu Ile Gly Leu Pro Thr Arg Leu Arg Asp Val 
            340                 345                 350         


Asp Val Ser Glu Glu Lys Leu Pro Asp Ile Ala Arg Asp Ala Phe Lys 
        355                 360                 365             


Ser Gly Met Met Lys Phe Asn Pro Arg Gln Pro Ser Leu Ser Glu Val 
    370                 375                 380                 


Leu Thr Leu Leu Gln Gln Ile Tyr 
385                 390         


<210>  2
<211>  383
<212>  PRT
<213>  Bacillus kribbensis

<400>  2

Met Asp Val Glu Phe Ser Phe His Leu Pro Thr Leu Ile Glu Phe Gly 
1               5                   10                  15      


Phe Gly Lys Ala Ser Leu Leu Gly Glu Arg Leu Leu Lys Leu Gly Val 
            20                  25                  30          


Gly Asn Val Phe Leu Val Ser Asp Lys Gly Val Ala Ser Ala Gly Leu 
        35                  40                  45              


Leu Gln Lys Leu Glu Gln Ser Leu Gln Thr Ser Asp Ile His Phe Lys 
    50                  55                  60                  


Thr Tyr Leu Glu Val Glu Pro Asp Pro Ser Leu Glu Thr Ile Asp Leu 
65                  70                  75                  80  


Gly Ala Glu Ala Phe Asn Ser Gly Lys Tyr Asp Cys Ile Val Ala Val 
                85                  90                  95      


Gly Gly Gly Ser Ala Ile Asp Thr Ala Lys Gly Ile Arg Val Val Ala 
            100                 105                 110         


Gly Asn Gly Gly Ser Ile Gly Asp Phe Ala Gly Val Asp Lys Ile Gly 
        115                 120                 125             


Lys Ala Pro Gln Ile Pro Leu Ile Ala Val Pro Thr Thr Ser Gly Thr 
    130                 135                 140                 


Gly Ser Glu Val Thr Ile Phe Gly Val Tyr Ser Asp Trp Val Lys Asn 
145                 150                 155                 160 


Val Lys Val Thr Val Thr Ser Gln Tyr Met Ala Pro Thr Ile Ala Leu 
                165                 170                 175     


Val Asp Pro Glu Leu Thr Met Arg Leu Pro Arg Lys Met Thr Ala Ala 
            180                 185                 190         


Ser Gly Ile Asp Ala Leu Ala His Gly Ile Glu Ser Tyr Phe Ser Leu 
        195                 200                 205             


Arg Ser Thr Ser Ala Ser Arg Ala Leu Ser Leu Glu Ala Ile Asn Ile 
    210                 215                 220                 


Val Gly Asn His Leu Arg Gln Ser Val Ala Asn Gly Glu Asp Lys Glu 
225                 230                 235                 240 


Ala Arg Cys Gly Met Ser His Gly Ser Leu Leu Ala Gly Met Ala Phe 
                245                 250                 255     


Asn Asn Gly Phe Leu Gly Leu Ala His Ala Ile Gly Ser Ala Leu Ser 
            260                 265                 270         


Gly His Cys His Val Pro His Gly Val Ala Ile Gly Leu Leu Leu Pro 
        275                 280                 285             


His Val Val Glu Phe Asn Ser Ser Glu Cys Pro Asp Gln Ala Ala Glu 
    290                 295                 300                 


Ile Ala Lys Ile Leu Gly Val Lys Ala Glu Asp Glu Arg Gln Leu Ala 
305                 310                 315                 320 


Glu Gln Ala Ser His Ala Val Gly Asp Leu Val Lys Asp Ile Gly Leu 
                325                 330                 335     


Pro Thr Arg Leu Arg Asp Met Asn Val Pro Glu Glu Lys Leu Ala Asp 
            340                 345                 350         


Ile Ala Arg Asp Ser Phe Gln Ser Gly Met Met Lys Phe Asn Pro Arg 
        355                 360                 365             


Arg Ala Ser Glu Ser Glu Val Leu Glu Leu Leu His Arg Val Tyr 
    370                 375                 380             


<210>  3
<211>  391
<212>  PRT
<213>  Geobacillus kaustophilus

<400>  3

Met Val Gly His Tyr Ile Gln Lys Glu Val Glu Phe Glu Phe Ser Phe 
1               5                   10                  15      


His Leu Pro Thr Ser Ile Gln Phe Gly Tyr Gly Lys Ala Ser Gln Leu 
            20                  25                  30          


Gly Asn Gln Leu Val Asp Met Gly Ile Lys Ser Ala Phe Leu Val Thr 
        35                  40                  45              


Asp Arg Gly Val Glu Ala Thr Gly Leu Leu Ala Gly Ile Ile Gln Ser 
    50                  55                  60                  


Leu Glu Ser Ser Asn Ile Gln Tyr Cys Val Tyr Ala Asp Val Glu Pro 
65                  70                  75                  80  


Asp Pro Ser Leu Glu Thr Ile Asp Gln Gly Ala Ala Ala Phe Lys Glu 
                85                  90                  95      


Gln Pro Phe Asp Cys Ile Val Ala Ile Gly Gly Gly Ser Pro Ile Asp 
            100                 105                 110         


Thr Ala Lys Gly Ile Arg Val Val Ala Thr Asn Gly Gly Ser Ile Gly 
        115                 120                 125             


Asp Tyr Ala Gly Val Asn Arg Ile Lys Lys Lys Ser Glu Ile Pro Leu 
    130                 135                 140                 


Ile Ala Leu Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Ile Phe 
145                 150                 155                 160 


Gly Val Tyr Ser Asp Trp Lys Asn Asn Val Lys Val Thr Val Thr Ser 
                165                 170                 175     


Pro Tyr Met Ala Pro Glu Ile Ala Leu Val Asp Pro Lys Leu Thr Met 
            180                 185                 190         


Ser Leu Pro Lys Lys Ile Thr Ala Ala Ser Gly Ile Asp Ala Leu Ala 
        195                 200                 205             


His Gly Ile Glu Thr Phe Phe Ser Leu Arg Ser Gln Pro Ile Ser Asp 
    210                 215                 220                 


Val Leu Ala Ile Glu Ala Met Thr Thr Val Asn Arg Tyr Leu Arg Arg 
225                 230                 235                 240 


Ala Val Glu Asp Gly Thr Asn Lys Glu Ala Arg Ile Gly Met Ser Tyr 
                245                 250                 255     


Gly Ser Leu Leu Ala Gly Met Ala Phe Asn Asn Gly Phe Leu Gly Leu 
            260                 265                 270         


Ala His Ala Ile Gly Ser Ala Leu Ser Gly His Cys His Val Ser His 
        275                 280                 285             


Gly Val Ala Ile Gly Leu Leu Leu Pro Lys Val Val Glu Phe Asn Ser 
    290                 295                 300                 


Val Val Gln Pro Glu Lys Ala Ala Lys Ile Ala Glu Leu Leu Gly Arg 
305                 310                 315                 320 


Lys Gly Asn Gln Asn Thr Leu Val Gln Gln Ala Ala Leu Ala Val Ala 
                325                 330                 335     


Ser Leu Val Lys Glu Ile Gly Leu Pro Thr Arg Leu Arg Asp Val Asp 
            340                 345                 350         


Val Pro Lys Glu Lys Leu Pro Asp Ile Ala Lys Asp Ser Phe Lys Ser 
        355                 360                 365             


Gly Met Met Arg Phe Asn Pro Arg Gln Pro Ser Glu Ala Glu Val Met 
    370                 375                 380                 


Thr Leu Leu Gln Gln Ile Tyr 
385                 390     


<210>  4
<211>  390
<212>  PRT
<213>  Aneurinibacillus terranovensis

<400>  4

Met Ser Pro Ala Val Lys Ala Ile Asn Phe Glu Phe Ser Phe Asn Leu 
1               5                   10                  15      


Pro Thr Leu Ile Glu Phe Gly Tyr Gly Lys Met Glu Lys Phe Gly Gln 
            20                  25                  30          


Gln Leu Ile Ser Ile Gly Val Lys Arg Ile Phe Met Val Thr Asp Lys 
        35                  40                  45              


Gly Val Glu Ser Ala Gly Leu Leu Ala Ala Leu Thr Asp Ser Leu Gln 
    50                  55                  60                  


Ala Ala Ala Ile Gln Phe Asp Ile Tyr Thr Asp Val Glu Ser Asp Pro 
65                  70                  75                  80  


Ser Leu Glu Thr Ile Asp Arg Gly Val Glu Val Phe Gln Gln Lys Pro 
                85                  90                  95      


Tyr Asp Cys Ile Val Ala Val Gly Gly Gly Ser Pro Ile Asp Thr Ala 
            100                 105                 110         


Lys Gly Ile Arg Val Val Ala Ala Asn Gly Gly Asn Ile Gly His Tyr 
        115                 120                 125             


Ala Gly Val Asn Gln Ile Pro Val Ala Pro Thr Ile Pro Leu Leu Ala 
    130                 135                 140                 


Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Asn Phe Gly Val 
145                 150                 155                 160 


Tyr Ser Asp Trp Gln Asn Asn Val Lys Val Thr Val Thr Ser Gln Tyr 
                165                 170                 175     


Met Ala Pro Thr Ile Ala Trp Val Asp Pro Ala Leu Thr Met Ser Leu 
            180                 185                 190         


Pro Ala Lys Met Thr Ala Ala Ser Gly Ile Asp Ala Leu Ala His Gly 
        195                 200                 205             


Ile Glu Thr Phe Phe Ser Leu Gly Ser Ser Pro Ala Ser Asp Ala Leu 
    210                 215                 220                 


Ala Ile Glu Ala Ile His Thr Val Asn Arg Tyr Leu Ser Arg Ala Val 
225                 230                 235                 240 


His Asn Gly Ser Asp Met Glu Ala Arg Ile Gly Met Ser His Gly Ser 
                245                 250                 255     


Leu Leu Ala Gly Met Ala Phe Asn Asn Gly Phe Leu Gly Leu Ala His 
            260                 265                 270         


Ala Ile Gly Ser Ala Leu Ser Gly His Cys His Val Pro His Gly Val 
        275                 280                 285             


Ala Ile Gly Leu Leu Leu Pro Lys Val Val Glu Phe Asn Ala Thr Val 
    290                 295                 300                 


Arg Pro Asp Lys Ala Ala Lys Ile Ala Gly Leu Met Gly Met Lys Gly 
305                 310                 315                 320 


Glu His Ser Glu Glu Leu Ala Leu Gln Ala Ser Pro Ala Val Ala Arg 
                325                 330                 335     


Leu Val Glu Asp Ile Gly Leu Pro Thr Arg Leu Arg Glu Val Asp Val 
            340                 345                 350         


Thr Glu Lys Lys Leu Phe Glu Ile Ala Lys Asp Ser Phe Lys Ser Gly 
        355                 360                 365             


Met Met Lys Phe Asn Pro Arg Gln Pro Ser Glu Ser Glu Val Leu Gln 
    370                 375                 380                 


Leu Leu Lys Glu Ile Phe 
385                 390 


<210>  5
<211>  390
<212>  PRT
<213>  Brevibacillus thermoruber

<400>  5

Met Ser Gln Thr Val Gln Gly Thr Asp Phe Ala Phe Ser Phe His Leu 
1               5                   10                  15      


Pro Thr Leu Ile Glu Phe Gly Tyr Gly Arg Ala Ser Arg Leu Gly Glu 
            20                  25                  30          


Arg Leu Gln His Leu Gly Val Thr Asn Val Phe Val Val Thr Asp Lys 
        35                  40                  45              


Gly Val Glu Ala Ala Gly Leu Leu Asn Gly Leu Val Gly Ser Leu Gln 
    50                  55                  60                  


Ser Ala Gly Ile Ala Phe Asp Leu Tyr Thr Glu Val Glu Pro Asp Pro 
65                  70                  75                  80  


Gly Leu Glu Thr Ile Asp Arg Gly Ala Ala Val Phe Arg Ala Lys Pro 
                85                  90                  95      


Tyr Asp Cys Leu Val Ala Val Gly Gly Gly Ser Pro Ile Asp Ala Ala 
            100                 105                 110         


Lys Gly Met Arg Val Val Thr Ser Cys Gly Gly Ser Ile Ala Asp Tyr 
        115                 120                 125             


Ala Gly Val Asn Arg Val Pro Met Ala Pro Ala Val Pro Leu Val Ala 
    130                 135                 140                 


Val Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Met Phe Gly Val 
145                 150                 155                 160 


Tyr Ser Asp Trp His Asn His Val Lys Val Thr Val Thr Ser Pro His 
                165                 170                 175     


Met Ala Pro Thr Ile Ala Leu Val Asp Pro Ala Leu Thr Val Ser Leu 
            180                 185                 190         


Pro Ala Lys Met Thr Ala Ala Ser Gly Ile Asp Ala Leu Ala His Gly 
        195                 200                 205             


Ile Glu Thr Phe Phe Ser Val Arg Ser Arg Pro Ala Ser Asp Ala Leu 
    210                 215                 220                 


Ala Met Glu Ala Ile Ala Ala Val Asn Ala His Leu Arg Arg Ala Val 
225                 230                 235                 240 


His Asp Gly Ser Asp Val Glu Ala Arg Ile Gly Met Ser His Gly Ser 
                245                 250                 255     


Leu Leu Ala Gly Met Ala Phe Thr Asn Gly Phe Leu Gly Leu Ala His 
            260                 265                 270         


Ala Ile Gly Ser Ala Leu Ser Gly His Cys His Val Pro His Gly Ile 
        275                 280                 285             


Ala Ile Gly Leu Leu Leu Pro His Val Val Ala Phe Asn Ala Pro Ala 
    290                 295                 300                 


Arg Pro Asp Lys Ala Ala Gln Leu Ala Arg Leu Leu Gly Val Glu Ala 
305                 310                 315                 320 


Asn Pro Arg Glu Glu Arg Gly Glu Glu Thr Ser Ala Ala Val Ala Arg 
                325                 330                 335     


Met Val Ala Asp Ile Gly Leu Pro Thr Arg Leu Arg Asp Val Gly Val 
            340                 345                 350         


Pro Glu Glu Lys Leu Pro Ala Ile Ala Lys Asp Ala Phe Lys Ser Gly 
        355                 360                 365             


Met Met Thr Cys Asn Pro Arg Gln Pro Thr Glu Gln Glu Val Arg Glu 
    370                 375                 380                 


Leu Leu Arg Arg Ala Phe 
385                 390 


<210>  6
<211>  379
<212>  PRT
<213>  Brevibacillus panacihumi

<400>  6

Met Glu Ser Pro Phe Ser Phe His Leu Pro Thr Asn Val Gln Phe Gly 
1               5                   10                  15      


Val Gly Ser Ala Ser Arg Leu Gly Glu Met Leu Leu Ser Met Gly Val 
            20                  25                  30          


Arg Arg Val Phe Leu Val Thr Asp Gln Gly Val Arg Gln Ala Gly Leu 
        35                  40                  45              


Leu Asp Glu Val Ile His Ser Leu Glu Glu Lys Gly Leu His Phe Gln 
    50                  55                  60                  


Ile Tyr Ala Asp Val Glu Pro Asp Pro Ser Leu Glu Thr Ile Gln Ala 
65                  70                  75                  80  


Gly Ala Ala Met Phe Gln Gln Gln Ser Phe Asp Cys Met Val Ala Ile 
                85                  90                  95      


Gly Gly Gly Ser Pro Ile Asp Thr Ala Lys Gly Ile Arg Val Leu Ala 
            100                 105                 110         


Ala Asn Gly Gly Gly Ile Gly Gln Tyr Ala Gly Val Asn Arg Val Pro 
        115                 120                 125             


Ala Ala Ser Ala Ile Pro Leu Ile Ala Ile Pro Thr Thr Ser Gly Thr 
    130                 135                 140                 


Gly Ser Glu Val Thr Ile Phe Gly Val Tyr Ser Asp Trp Glu Asn His 
145                 150                 155                 160 


Val Lys Ile Thr Val Thr Ser Pro His Met Ala Pro Ser Thr Ala Leu 
                165                 170                 175     


Ile Asp Pro Ala Leu Thr Leu Ser Leu Pro Ala Lys Met Thr Ala Ala 
            180                 185                 190         


Thr Gly Ile Asp Ala Leu Ala His Gly Ile Glu Thr Phe Phe Ser Leu 
        195                 200                 205             


Arg Ser Ser Pro Ala Ser Asp Ala Leu Ala Ile His Ala Met Lys Met 
    210                 215                 220                 


Ile Ala Pro His Leu Arg Arg Ala Val Arg Asp Gly Ala Asp Met Glu 
225                 230                 235                 240 


Ala Arg Ile Gly Met Ser Gln Gly Ser Val Leu Ala Gly Met Ala Phe 
                245                 250                 255     


Asn Asn Gly Phe Leu Gly Leu Ala His Ala Ile Gly Ser Ala Leu Ser 
            260                 265                 270         


Gly His Cys His Val Pro His Gly Val Ala Ile Gly Leu Leu Leu Pro 
        275                 280                 285             


His Val Val Ala Phe Asn Thr Pro Val Arg Pro Glu Lys Ala Glu Leu 
    290                 295                 300                 


Ile Ala Asp Val Leu Gly Ser Val Gln Lys Glu Thr Gly Thr Ala Ala 
305                 310                 315                 320 


Glu Leu Val Gly Gln Leu Val Gln Asp Ile Gly Leu Pro Gln Arg Leu 
                325                 330                 335     


Gln Glu Val Gly Val Pro Glu Ala Lys Leu Val Asp Ile Ala Lys Asp 
            340                 345                 350         


Ser Phe Lys Ser Gly Met Met Lys Trp Asn Pro Arg Leu Pro Thr Glu 
        355                 360                 365             


Gln Glu Val Leu Glu Leu Leu Gln Lys Ala Phe 
    370                 375                 


<210>  7
<211>  383
<212>  PRT
<213>  Bacillus sp. FJAT-14578

<400>  7

Met Tyr Pro Ser Phe Glu Phe His Leu Pro Thr Lys Ile His Phe Gly 
1               5                   10                  15      


Tyr Asn Thr Ile Lys Gln Leu Asp His Leu Pro Phe Glu Ile Lys Arg 
            20                  25                  30          


Ala Phe Ile Val Thr Asp Gln Gly Val Leu Asn Ser Gly Leu Val Glu 
        35                  40                  45              


Asn Val Thr Asn Ile Leu Lys Asp His Gln Ile Ser Tyr Val Ile Tyr 
    50                  55                  60                  


Ser Glu Val Glu Pro Asp Pro Ser Val Glu Thr Val Asp Lys Ala Ala 
65                  70                  75                  80  


Gln Met Phe Gln Arg Glu Glu Ala Asp Ala Leu Ile Ala Ile Gly Gly 
                85                  90                  95      


Gly Ser Pro Ile Asp Thr Ala Lys Gly Val Arg Val Ile Ala Gly Asn 
            100                 105                 110         


Gly Gly Ser Ile Arg Asp Tyr Ala Gly Val Asn Leu Ile Lys Gln Lys 
        115                 120                 125             


Ser Asn Ile Pro Leu Ile Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser 
    130                 135                 140                 


Glu Val Thr Ile Phe Ala Val Phe Ser Asp Trp Glu Glu Asn Arg Lys 
145                 150                 155                 160 


Val Thr Val Thr Ser Pro Phe Leu Ala Pro Asp Ile Ser Ile Val Asp 
                165                 170                 175     


Pro Lys Met Thr Met Thr Ala Pro Pro Ala Ile Thr Ala Ala Ser Gly 
            180                 185                 190         


Phe Asp Ala Phe Ala His Gly Ala Glu Thr Phe Val Ser Arg Ala Ser 
        195                 200                 205             


Gln Pro Ala Ser Asp Val Leu Ala Phe Ser Ala Met Ser Thr Val Ser 
    210                 215                 220                 


Lys Tyr Leu Arg Arg Ala Val Tyr Asn Gly Glu Asp Val Glu Ala Arg 
225                 230                 235                 240 


Ile Lys Met Ala Glu Ala Ser Leu Leu Ala Gly Met Ala Phe Asn Gln 
                245                 250                 255     


Ser Tyr Leu Gly Leu Thr His Ala Ile Gly Ser Ala Leu Ser Gly His 
            260                 265                 270         


Ala His Val Ser His Gly Val Ala Ile Gly Leu Leu Leu Pro Gly Val 
        275                 280                 285             


Ile Arg Tyr Asn Ser Ile Ser Arg Met Asp Lys His Ile Glu Met Ala 
    290                 295                 300                 


Gly Ala Phe Arg Glu Ile Asp Arg Ser Leu Ser Asp Trp Glu Ile Ile 
305                 310                 315                 320 


Asp Gln Leu Ile Glu Asp Val Ser Arg Leu Arg Asp Asp Ile Gly Leu 
                325                 330                 335     


Pro Gln Arg Leu Gln Gln Val Gly Val Lys Glu Asp Gln Leu Lys Met 
            340                 345                 350         


Ile Ala Ala Asp Ser Val Lys Ser Gly Met Trp Lys Phe Asn Pro Arg 
        355                 360                 365             


Gln Ala Ser Glu Glu Glu Ile Leu Glu Leu Leu Lys Glu Leu Tyr 
    370                 375                 380             


<210>  8
<211>  384
<212>  PRT
<213>  Desulfotomaculum kuznetsovii

<400>  8

Met Glu Ala Phe Thr Phe Gln Leu Lys Thr Thr Val Cys Phe Gly Ala 
1               5                   10                  15      


Asn Val Val Ser Gly Ile Val Asp Trp Cys Arg Asn Tyr Asn Ala Lys 
            20                  25                  30          


Arg Val Leu Ile Val Thr Asp Gln Gly Val Arg Lys Ala Gly Ile Leu 
        35                  40                  45              


Glu Lys Val Glu Lys Ile Leu Ser Asp Ala Gly Ile Glu Asn Val Val 
    50                  55                  60                  


Phe Asp Asp Val Glu Pro Asp Pro Gly Leu Glu Thr Ile His Arg Cys 
65                  70                  75                  80  


Ala Ser Cys Phe Arg Glu Asn Lys Cys Asp Leu Ile Leu Ala Val Gly 
                85                  90                  95      


Gly Gly Ser Pro Ile Asp Thr Ala Lys Gly Ala Arg Val Ile Val Glu 
            100                 105                 110         


Asn Gly Gly His Ile Arg Asp Tyr Ala Gly Val Asn Lys Val Pro Arg 
        115                 120                 125             


Ala Pro Val Thr Pro Leu Ile Ala Ile Pro Thr Thr Ser Gly Thr Gly 
    130                 135                 140                 


Ser Glu Val Thr Thr Phe Ala Val Leu Ser Asp Trp Glu Asn Arg Met 
145                 150                 155                 160 


Lys Ile Thr Ile Ser Ser Pro Phe Leu Ala Pro Glu Val Ala Val Val 
                165                 170                 175     


Asp Pro Leu Leu Thr Met Thr Ala Pro Pro Ser Val Thr Ala Ala Ser 
            180                 185                 190         


Gly Ile Asp Ala Leu Ser His Ala Ile Glu Thr Tyr Val Ser Leu Lys 
        195                 200                 205             


Ala Gln Pro Pro Ala Glu Ala Leu Ala Leu Lys Ala Ile Glu Leu Ile 
    210                 215                 220                 


Gly Glu Ser Leu Arg Thr Ala Val Ala Asp Gly Ser Asp Lys Glu Ala 
225                 230                 235                 240 


Arg Thr Arg Met Ser Leu Gly Ser Leu Leu Ala Gly Met Ala Phe Asn 
                245                 250                 255     


Asn Ser Leu Leu Gly Leu Thr His Ser Ile Gly Ala Ala Leu Ser Gly 
            260                 265                 270         


His Ala His Val Ser His Gly Met Ala Ile Gly Leu Leu Leu Pro Tyr 
        275                 280                 285             


Val Met Glu Phe Asn Ala Met Ala Arg Met Glu Lys Phe Ser Lys Ile 
    290                 295                 300                 


Ala Val Ala Leu Gly Glu Asp Val Lys Gly Leu Ser Leu Arg Glu Ala 
305                 310                 315                 320 


Ala Leu Arg Ser Val Lys Ala Val Arg Glu Leu Val Glu Asp Ile Ser 
                325                 330                 335     


Leu Pro Arg Arg Leu Gly Asp Val Gly Val Thr Gly Asp Met Ile Glu 
            340                 345                 350         


Gly Met Ala Lys Asp Ala Met Gly His Gly Met Leu Lys Phe Asn Pro 
        355                 360                 365             


Arg Ala Val Thr Glu Lys Asp Ile Ile Ala Ile Leu Arg Lys Ala Leu 
    370                 375                 380                 


<210>  9
<211>  377
<212>  PRT
<213>  Desulfurispora thermophila

<400>  9

Met Lys Thr Thr Val Cys Phe Gly Ala Asn Ile Val Ser Ser Ile Asp 
1               5                   10                  15      


Asp Arg Cys Arg Asp Tyr Asn Ala Arg His Val Leu Ile Val Thr Asp 
            20                  25                  30          


Gln Gly Val Glu Lys Ala Gly Ile Leu Glu Lys Val Glu Lys Val Leu 
        35                  40                  45              


Ser Asp Ala Gly Ile Glu Asn Val Val Phe Asp Asp Val Glu Pro Asp 
    50                  55                  60                  


Pro Gly Leu Glu Thr Ile His Arg Cys Ala Ser Cys Phe Arg Glu Asn 
65                  70                  75                  80  


Lys Cys Asp Leu Phe Leu Ala Ile Gly Gly Gly Ser Pro Ile Asp Thr 
                85                  90                  95      


Ala Lys Gly Ala Arg Ile Ile Val Asp Asn Gly Gly His Ile Arg Asp 
            100                 105                 110         


Tyr Ala Gly Val Asn Lys Val Pro Arg Ala Pro Arg Thr Pro Leu Leu 
        115                 120                 125             


Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Thr Phe Ala 
    130                 135                 140                 


Val Leu Ser Asp Trp Glu Asn Arg Met Lys Ile Thr Ile Ser Ser Pro 
145                 150                 155                 160 


Phe Leu Ala Pro Glu Val Ala Val Val Asp Pro Ile Leu Thr Leu Thr 
                165                 170                 175     


Ala Pro Pro Ser Val Thr Ala Ala Ser Gly Ile Asp Ala Leu Ser His 
            180                 185                 190         


Ala Ile Glu Thr Tyr Val Ser Leu Lys Ala Gln Pro Pro Ala Glu Ala 
        195                 200                 205             


Leu Ala Leu Lys Ala Ile Glu Leu Ile Gly Glu Ser Leu Arg Ala Ala 
    210                 215                 220                 


Val Ala Asp Gly Ser Asn Lys Glu Ala Arg Thr Lys Met Ser Leu Gly 
225                 230                 235                 240 


Ser Leu Leu Ala Gly Met Ala Phe Asn Asn Ser Leu Leu Gly Leu Thr 
                245                 250                 255     


His Ser Ile Gly Ala Ala Leu Ser Gly His Ala His Val Ser His Gly 
            260                 265                 270         


Met Ala Val Gly Leu Leu Leu Pro Tyr Val Met Glu Phe Asn Ala Met 
        275                 280                 285             


Ala Arg Leu Glu Lys Tyr Gly Lys Ile Ala Ile Ala Leu Gly Glu Asp 
    290                 295                 300                 


Val Lys Gly Leu Ser Leu Arg Glu Ala Ala Leu Arg Ser Val Lys Ala 
305                 310                 315                 320 


Val Arg Glu Leu Val Glu Asp Ile Ser Leu Pro Arg Arg Leu Gly Glu 
                325                 330                 335     


Val Gly Val Thr Gly Asp Met Ile Glu Gly Met Ala Lys Asp Ala Met 
            340                 345                 350         


Gly His Gly Met Leu Lys Phe Asn Pro Arg Val Val Thr Glu Lys Asp 
        355                 360                 365             


Ile Met Ala Ile Leu Gln Lys Ala Leu 
    370                 375         


<210>  10
<211>  383
<212>  PRT
<213>  Bacillus sp. L1(2012)

<400>  10

Met Tyr Thr Ser Phe Asn Phe His Leu Pro Thr Arg Ile Gln Phe Gly 
1               5                   10                  15      


Tyr Glu Lys Val Lys Glu Leu Lys Asn Leu Pro Phe Gln Ala Asn Arg 
            20                  25                  30          


Ala Phe Ile Val Thr Asp Lys Gly Val Glu Lys Ala Gly Leu Leu Asn 
        35                  40                  45              


Asp Val Ile Asp Ala Ile Lys Gln Ala Asn Met Thr Tyr Lys Ile Tyr 
    50                  55                  60                  


Arg Asp Val Glu Pro Asp Pro Ser Val Glu Thr Val Asp Lys Ala Ala 
65                  70                  75                  80  


Lys Ala Phe Ala Glu Ala Glu Cys Asp Leu Leu Ile Ala Val Gly Gly 
                85                  90                  95      


Gly Ser Pro Ile Asp Thr Ala Lys Gly Val Arg Val Val Ala Ser Asn 
            100                 105                 110         


Gly Gly Ser Ile Arg Asn Tyr Ser Gly Val Asn Leu Val Lys Glu Ala 
        115                 120                 125             


Pro Ser Val Pro Leu Val Ala Ile Pro Thr Thr Ala Gly Thr Gly Ser 
    130                 135                 140                 


Glu Val Thr Ile Phe Ala Val Phe Ser Asp Asp Lys Glu Asn Arg Lys 
145                 150                 155                 160 


Val Thr Val Thr Ser Ser His Leu Ser Pro Asp Val Ser Ile Ile Asp 
                165                 170                 175     


Pro Lys Leu Thr Leu Thr Ala Pro Pro Ser Ile Thr Ala Ala Ala Gly 
            180                 185                 190         


Phe Asp Ala Phe Ala His Ala Ala Glu Ala Phe Val Ser Arg Ile Ser 
        195                 200                 205             


Gln Pro Pro Ser Asp Ala Leu Ala Leu Ser Ala Met Lys Thr Val His 
    210                 215                 220                 


Thr Tyr Leu Arg Arg Ala Val Tyr Asn Gly Asp Asp Ile Glu Ala Arg 
225                 230                 235                 240 


Met Lys Met Ala Glu Ala Ser Leu Leu Ala Gly Met Ala Phe Asn Gln 
                245                 250                 255     


Ser Tyr Leu Gly Leu Ala His Ala Ile Gly Ser Ala Ile Ser Val His 
            260                 265                 270         


Ala His Val Ser His Gly Val Val Ile Gly Leu Leu Leu Pro Lys Val 
        275                 280                 285             


Ile Glu Tyr Asn Leu Val Ala Lys Ile Asp Lys Tyr Ala Glu Ala Gly 
    290                 295                 300                 


Lys Tyr Ile Glu Gln Ser Ser His Gly Leu Ser Asn Tyr Glu Ala Ala 
305                 310                 315                 320 


Ala Leu Phe Ser Glu Thr Val Thr Gln Leu Arg Asn Asp Ile Gly Leu 
                325                 330                 335     


Pro Lys Gln Leu Arg Glu Val Asn Val Lys Glu Ala Gln Leu Glu Ala 
            340                 345                 350         


Ile Ser Lys Asp Ser Ile Lys Ser Gly Met Trp Gln Phe Asn Pro Arg 
        355                 360                 365             


Arg Ala Ser Glu Gln Asp Val Tyr Gln Met Leu Arg Glu Met Leu 
    370                 375                 380             


<210>  11
<211>  387
<212>  PRT
<213>  Pelotomaculum thermopropionicum

<400>  11

Met Ala Asp Tyr Asn Phe Ser Phe Ala Val Arg Thr Lys Val Phe Phe 
1               5                   10                  15      


Gly Arg Gly Val Val Phe Glu Gln Leu Pro Gly Ala Val Arg Glu Met 
            20                  25                  30          


Gly Cys Lys Lys Ala Val Leu Val Ser Asp Pro Gly Ile Val Gly Thr 
        35                  40                  45              


Gly Leu Ala Asp Arg Val Lys Asp Leu Leu Ala Gly Gly Gly Val Ala 
    50                  55                  60                  


Val Glu Val Phe Ser Glu Val Glu Pro Asp Pro Gly Leu Glu Thr Val 
65                  70                  75                  80  


His Lys Ala Ala Ala Phe Leu Gly Arg Thr Arg Pro Asp Cys Leu Val 
                85                  90                  95      


Ala Leu Gly Gly Gly Ser Ser Ile Asp Val Ala Lys Gly Ala Arg Val 
            100                 105                 110         


Ile Tyr Asp Asn Gly Gly Lys Ile Ser Asp Tyr Ala Gly Val Asn Lys 
        115                 120                 125             


Val Lys Val Lys Pro Ser Leu Pro Leu Met Ala Val Pro Thr Thr Ala 
    130                 135                 140                 


Gly Thr Gly Ser Glu Val Thr Val Phe Ala Val Leu Ser Asp Trp Glu 
145                 150                 155                 160 


Gln Asn Ile Lys Ile Thr Val Thr Ser Glu Tyr Leu Ala Pro Glu Ala 
                165                 170                 175     


Ala Phe Val Asp Pro Leu Ala Met Val Ser Ala Pro Pro Gly Ile Thr 
            180                 185                 190         


Ala Ala Ser Gly Ile Asp Ala Leu Ser His Ala Val Glu Ala Tyr Val 
        195                 200                 205             


Ser Arg Ala Ala Ser Pro Val Ser Asp Asn Leu Ala Leu Gly Ala Val 
    210                 215                 220                 


Glu Leu Ile Gly Gly His Leu Arg Gln Ala Val Ala Asn Gly Gly Asp 
225                 230                 235                 240 


Leu Ala Ala Arg Thr Gly Ala Ala Leu Gly Ser Leu Leu Ala Gly Met 
                245                 250                 255     


Ala Phe Asn Asn Ala Phe Leu Gly Leu Thr His Ser Ile Gly Ala Ala 
            260                 265                 270         


Leu Ser Gly His Val His Val Ser His Gly Val Ala Val Gly Leu Leu 
        275                 280                 285             


Leu Pro Tyr Val Met Glu Tyr Asn Leu Met Ala Lys Pro Asp Lys Phe 
    290                 295                 300                 


Ala Arg Leu Ala Arg Ala Met Gly Glu Val Thr Glu Gly Lys Ser Leu 
305                 310                 315                 320 


Tyr Arg Ala Ala Ser Leu Ala Pro Arg Ala Val Lys Ala Met Val Lys 
                325                 330                 335     


Ser Ile Gly Leu Pro Val Arg Leu Lys Glu Ile Gly Val Pro Glu Gly 
            340                 345                 350         


Ala Leu Ala Ala Ile Ala Glu Thr Ala Leu Lys His Gly Met Ile Lys 
        355                 360                 365             


Phe Asn Pro Arg Val Pro Ser Arg Glu Asp Ile Leu Asp Ile Val Lys 
    370                 375                 380                 


Lys Ala Tyr 
385         


<210>  12
<211>  1176
<212>  DNA
<213>  Aeribacillus pallidus

<400>  12
atgatcggaa attacgcaaa aaaggcgatt gatttcgagt tcacttttta tcttcctaca     60

ttgatcgaat tcggatacgg caaggcttcc cgaatgggag agatgcttga acagatgggt    120

ataaagaacg tttttttggt taccgacaaa ggagtggaag ctgcgggtct gttggcagga    180

atcgttcagt ctctggaatc atccaatatc cgatatgtta tttattcaga cgtagaacct    240

gacccgagct tagagacgat tgatcgtggt gcgtccgttt ttaaggagca gtcttttgac    300

tgtatcttag ctgtgggtgg aggaagtccg attgatacag ctaaggggat ccgtgtcgta    360

gtgacgaacg gaggaaacat cggtgactat gccggtgtta accgtgttgc gaaaaaatct    420

gaaattcctt tggtggctgt gccgactaca tccggcacgg gcagtgaagt aaccattttc    480

ggagtctact ccgattggga aaatcaagta aaggtgacgg taacaagccc atatatggcg    540

ccggagatcg ctttggtaga ccccgaactt accatgagtc taccgcaaaa aatgacagca    600

gcatcgggaa ttgatgctct agctcatggg attgaaactt tcttctcctt gcgttctcga    660

cctgcatccg atgccctagc ggtcgaagcg atggcgacgg tgagtgctta tttgcgccgt    720

gcggtggaag atggtacgga taaagaagcg aggatcggca tgtcccaggg cagtttgttg    780

gcagggatgg cattcaacaa tggcttctta ggtttggccc atgcgatcgg tagtgctttg    840

tctggccatt gtcatgtgtc ccatggtgtc gcaatcggtt tgttgctacc gaaagtggtg    900

gaatttaatg ctagggtgcg cccggaaaaa gctgcaaaaa tcgcagaatt gttgggagta    960

aaaggggatc gagaggaggt tcttgcggag caggcagctc ctgcagtcgc ctcgttagtc   1020

aaagagattg gtcttcccac tcgtttgcgt gatgttgatg tttctgaaga aaagctccca   1080

gatatcgcaa gagatgcatt taaaagcggt atgatgaagt ttaacccacg ccaaccaagt   1140

ttgtcagaag tgcttacact tttgcagcag atttat                             1176


<210>  13
<211>  1179
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic codon optimised for expression in P. putida

<400>  13
atgatcggca actacgccaa gaaggccatc gacttcgagt tcaccttcta cctgccgacc     60

ctgatcgagt tcggctacgg caaggccagc cgcatgggcg agatgctgga acaaatgggt    120

atcaagaacg tgttcctggt gaccgacaag ggcgtggaag ccgccggtct gctggccggc    180

atcgtgcaga gcctggaaag cagcaacatc cgctacgtga tctacagcga cgtggaaccg    240

gacccgagcc tggaaaccat cgaccgcggc gccagcgtgt tcaaagaaca gagcttcgac    300

tgcatcctgg ccgtgggcgg cggcagcccg atcgacaccg ccaagggcat ccgcgtggtg    360

gtgaccaacg gcggcaacat cggcgactac gccggcgtga accgcgtggc caagaagtcg    420

gagatcccgc tggtcgccgt gccaaccacc tcgggcaccg gcagcgaagt gaccatcttc    480

ggcgtgtaca gcgactggga gaaccaggtg aaggtgaccg tgaccagccc gtacatggcc    540

ccggaaatcg ccctggtgga cccggaactg accatgagcc tgccgcagaa gatgaccgcc    600

gccagcggca tcgacgccct ggcccacggc atcgaaacct tcttcagcct gcgcagccgc    660

ccagcctcgg atgccctggc ggtggaagcc atggccaccg tgagcgccta cctgcgccgc    720

gccgtcgagg acggcaccga caaagaagcc cgcatcggca tgagccaggg cagcctgctg    780

gcgggcatgg ccttcaacaa cggcttcctg ggcctggccc atgccatcgg cagcgccctg    840

agcggccatt gccatgtgag ccacggcgtg gccatcggcc tgctgctgcc gaaggtggtg    900

gaattcaacg cccgcgtgcg cccggaaaag gccgccaaga tcgccgaact gctgggcgtg    960

aagggcgacc gcgaagaggt gctggccgaa caggccgccc cagccgtggc cagcctggtg   1020

aaagaaatcg gcctgccgac ccgcctgcgc gacgtggacg tgagcgaaga gaagctgccg   1080

gacatcgccc gcgacgcctt caagagcggc atgatgaagt tcaacccgcg ccagccgagc   1140

ctgagcgagg tgctgaccct gctgcagcag atctactga                          1179


<210>  14
<211>  1152
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic codon optimised for expression in P.putida

<400>  14
atggacgtgg aattcagctt ccatctgccg accctgatcg agttcggctt cggcaaggcc     60

agcctgctgg gcgagcgcct gctgaagctg ggcgtgggca acgtgttcct ggtgagcgac    120

aagggcgtgg ccagcgcagg cctgctgcag aagctggaac agagcctgca gaccagcgac    180

atccacttca agacctacct ggaagtggaa ccggacccga gcctggaaac catcgacctg    240

ggtgccgagg ccttcaacag cggcaagtac gactgcatcg tggccgtggg tggtggcagc    300

gccatcgaca ccgccaaggg catccgcgtg gtggcaggca acggtggcag catcggcgac    360

ttcgcaggcg tggacaagat cggcaaggca ccgcagatcc cgctgatcgc cgtgccgacc    420

acctcgggca ccggcagcga agtgaccatc ttcggcgtgt acagcgactg ggtgaagaac    480

gtgaaggtga ccgtgaccag ccagtacatg gcaccgacca ttgccctggt ggacccggaa    540

ctgaccatgc gcctgccacg caagatgacc gcagccagcg gcatcgacgc cctggcccac    600

ggcatcgaga gctacttcag cctgcgcagc accagcgcca gccgtgccct gtcgctggaa    660

gccatcaaca tcgtgggcaa ccatctgcgc cagagcgtgg cgaacggcga ggacaaggaa    720

gcacgctgcg gcatgagcca cggcagcctg ctggcaggca tggcgttcaa caacggcttc    780

ctgggcctgg cccatgccat cggcagcgca ctgagcggtc actgccacgt gccgcacggc    840

gtggccatcg gcctgctgct gccgcacgtg gtggaattca acagcagcga gtgcccagac    900

caggcagccg agatcgccaa gatcctgggc gtgaaggccg aggacgaacg ccagctggcc    960

gaacaggcca gccacgccgt gggcgacctg gtgaaggaca tcggcctgcc gacccgtctg   1020

cgcgacatga acgtgccgga agagaagctg gccgacattg cacgcgacag cttccagagc   1080

ggcatgatga agttcaaccc acgtcgtgcc agcgagagcg aggtgctgga actgctgcac   1140

cgcgtgtact ga                                                       1152


<210>  15
<211>  1994
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic XbaI-SalI fragment

<400>  15
tctagatctt ctttgataat aaatgaaagc agccggtatg gagagaaaaa agtgcactta     60

tatgaagttg attttatggt cggctttatt ttgcccgtcg tactggctgt ccacacgatg    120

ttcatttttg atgcacaatt gaatggctgt acagttgcgt ttttgtcgat gtctggcggg    180

cacgcctcca tgcatgtgaa gcagattctt ttaagcgggc agcacccgct tttttggagg    240

gcaggcattc aggagcaaaa atggcagaga tcagttgggc gggatcagcc atttattcct    300

ccatccgggg cactttgtga aaatcagcac aagaatgaat aacgctttca tatctggctt    360

tttcaaataa aaccatttgt gaaaaatgta aacggatgat tttgaaaaac cgtcattttc    420

ctttaaaacc gggcatttgg gcagataaat tttcaaattt tcgccataaa atatgtgaat    480

ctaatcacaa aaatagtggt atacttaccc atgtggaatg aaggaaaatg aacggaacga    540

tccatttcag ccataaaagg gcatgccgtc catctatttc acaaaccgca cggcagcatt    600

tgctgcaaaa gtttaatcgt cctgctttaa aggaaaagca gtatggaatc cattaggagt    660

tggcacaata tccatagact ggataggggc ccgccatgcc gggcttgcaa aactgctttc    720

atacagtgga aatatttttt acttttgatg gggaggaaga ttatatatga tcggaaatta    780

cgcaaaaaag gcgattgatt tcgagttcac tttttatctt cctacattga tcgaattcgg    840

atacggcaag gcttcccgaa tgggagagat gcttgaacag atgggtataa agaacgtttt    900

tttggttacc gacaaaggag tggaagctgc gggtctgttg gcaggaatcg ttcagtctct    960

ggaatcatcc aatatccgat atgttattta ttcagacgta gaacctgacc cgagcttaga   1020

gacgattgat cgtggtgcgt ccgtttttaa ggagcagtct tttgactgta tcttagctgt   1080

gggtggagga agtccgattg atacagctaa ggggatccgt gtcgtagtga cgaacggagg   1140

aaacatcggt gactatgccg gtgttaaccg tgttgcgaaa aaatctgaaa ttcctttggt   1200

ggctgtgccg actacatccg gcacgggcag tgaagtaacc attttcggag tctactccga   1260

ttgggaaaat caagtaaagg tgacggtaac aagcccatat atggcgccgg agatcgcttt   1320

ggtagacccc gaacttacca tgagtctacc gcaaaaaatg acagcagcat cgggaattga   1380

tgctctagct catgggattg aaactttctt ctccttgcgt tctcgacctg catccgatgc   1440

cctagcggtc gaagcgatgg cgacggtgag tgcttatttg cgccgtgcgg tggaagatgg   1500

tacggataaa gaagcgagga tcggcatgtc ccagggcagt ttgttggcag ggatggcatt   1560

caacaatggc ttcttaggtt tggcccatgc gatcggtagt gctttgtctg gccattgtca   1620

tgtgtcccat ggtgtcgcaa tcggtttgtt gctaccgaaa gtggtggaat ttaatgctag   1680

ggtgcgcccg gaaaaagctg caaaaatcgc agaattgttg ggagtaaaag gggatcgaga   1740

ggaggttctt gcggagcagg cagctcctgc agtcgcctcg ttagtcaaag agattggtct   1800

tcccactcgt ttgcgtgatg ttgatgtttc tgaagaaaag ctcccagata tcgcaagaga   1860

tgcatttaaa agcggtatga tgaagtttaa cccacgccaa ccaagtttgt cagaagtgct   1920

tacacttttg cagcagattt attaattgtt cgggtttcag tgttccattt tcaaatattc   1980

cgttaagggt cgac                                                     1994


<210>  16
<211>  1258
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-XbaI fragment

<400>  16
ggtaccttca cacaggaaac aggaggtaca atgatcggaa attacgcaaa aaaggcgatt     60

gatttcgagt tcacttttta tcttcctaca ttgatcgaat tcggatacgg caaggcttcc    120

cgaatgggag agatgcttga acagatgggt ataaagaacg tttttttggt taccgacaaa    180

ggagtggaag ctgcgggtct gttggcagga atcgttcagt ctctggaatc atccaatatc    240

cgatatgtta tttattcaga cgtagaacct gacccgagct tagagacgat tgatcgtggt    300

gcgtccgttt ttaaggagca gtcttttgac tgtatcttag ctgtgggtgg aggaagtccg    360

attgatacag ctaaggggat ccgtgtcgta gtgacgaacg gaggaaacat cggtgactat    420

gccggtgtta accgtgttgc gaaaaaatct gaaattcctt tggtggctgt gccgactaca    480

tccggcacgg gcagtgaagt aaccattttc ggagtctact ccgattggga aaatcaagta    540

aaggtgacgg taacaagccc atatatggcg ccggagatcg ctttggtaga ccccgaactt    600

accatgagtc taccgcaaaa aatgacagca gcatcgggaa ttgatgctct agctcatggg    660

attgaaactt tcttctcctt gcgttctcga cctgcatccg atgccctagc ggtcgaagcg    720

atggcgacgg tgagtgctta tttgcgccgt gcggtggaag atggtacgga taaagaagcg    780

aggatcggca tgtcccaggg cagtttgttg gcagggatgg cattcaacaa tggcttctta    840

ggtttggccc atgcgatcgg tagtgctttg tctggccatt gtcatgtgtc ccatggtgtc    900

gcaatcggtt tgttgctacc gaaagtggtg gaatttaatg ctagggtgcg cccggaaaaa    960

gctgcaaaaa tcgcagaatt gttgggagta aaaggggatc gagaggaggt tcttgcggag   1020

caggcagctc ctgcagtcgc ctcgttagtc aaagagattg gtcttcccac tcgtttgcgt   1080

gatgttgatg tttctgaaga aaagctccca gatatcgcaa gagatgcatt taaaagcggt   1140

atgatgaagt ttaacccacg ccaaccaagt ttgtcagaag tgcttacact tttgcagcag   1200

atttattaat tgttcgggtt tcagtgttcc attttcaaat attccgttaa ggtctaga     1258


<210>  17
<211>  446
<212>  PRT
<213>  Aeribacillus pallidus

<400>  17

Met Lys Asn Ile Ala Asn Thr Ser Thr Glu Arg Pro Val Asn Asp Ala 
1               5                   10                  15      


Ser Val Lys Asn Arg Gln Met Val Arg Ala Thr Ile Ala Ser Leu Ile 
            20                  25                  30          


Gly Trp Ser Leu Asp Leu Tyr Asp Leu Phe Leu Leu Leu Phe Val Ala 
        35                  40                  45              


Thr Thr Ile Gly Asn Leu Phe Phe Pro Ala Ser Asn Gln Thr Leu Ser 
    50                  55                  60                  


Leu Ala Ala Val Tyr Ala Ser Phe Ala Val Thr Leu Leu Met Arg Pro 
65                  70                  75                  80  


Leu Gly Ser Ala Ile Phe Gly Ile Tyr Ala Asp Lys Asn Gly Arg Lys 
                85                  90                  95      


Lys Ala Met Thr Val Ala Ile Ile Gly Ala Gly Leu Cys Thr Ala Ala 
            100                 105                 110         


Phe Gly Leu Leu Pro Thr Ile His Gln Val Gly Val Val Ala Ala Ile 
        115                 120                 125             


Ala Phe Leu Ile Leu Arg Leu Val Gln Gly Val Phe Val Gly Gly Val 
    130                 135                 140                 


Val Ala Ser Thr His Thr Ile Gly Thr Glu Ser Ala Ser Pro Lys Tyr 
145                 150                 155                 160 


Arg Gly Phe Met Ser Gly Leu Ile Gly Gly Gly Gly Ala Gly Leu Gly 
                165                 170                 175     


Ala Leu Phe Ala Ser Ile Ser Tyr Ser Val Val Thr Ala Ile Phe Pro 
            180                 185                 190         


Gly Glu Ala Phe Asp Val Trp Gly Trp Arg Val Met Phe Phe Thr Gly 
        195                 200                 205             


Ile Ile Gly Ser Leu Phe Gly Leu Phe Ile Phe Arg Ser Leu Glu Glu 
    210                 215                 220                 


Ser Pro Leu Trp Lys Gln Leu Lys Glu Glu Asn Ser Lys Gly Glu Val 
225                 230                 235                 240 


Ser Glu Phe Gln Lys Ala Pro Leu Lys Thr Phe Phe Thr Lys Tyr Tyr 
                245                 250                 255     


Lys Val Leu Leu Val Asn Leu Met Ile Val Ile Gly Gly Gly Ser Gly 
            260                 265                 270         


Tyr Tyr Leu Thr Ser Gly Phe Ile Pro Thr Phe Leu Lys Val Val Asn 
        275                 280                 285             


Lys Val Ser Ala Ser Val Ser Ser Gly Val Leu Ile Ala Thr Ser Ile 
    290                 295                 300                 


Met Thr Ile Val Ala Ala Val Leu Val Gly His Leu Ser Glu Val Ile 
305                 310                 315                 320 


Gly Arg Lys Lys Thr Phe Leu Leu Ile Gly Ile Leu Cys Leu Val Gly 
                325                 330                 335     


Leu Pro Tyr Phe Tyr Leu Ser Leu Ala Asn Ser Thr Thr Thr Thr Gly 
            340                 345                 350         


Ile Tyr Leu Asn Ala Leu Gly Leu Ile Phe Leu Gly Asn Ala Ala Tyr 
        355                 360                 365             


Ala Pro Val Leu Ile Phe Leu Asn Glu Arg Phe Pro Thr Ser Ile Arg 
    370                 375                 380                 


Ser Thr Gly Thr Gly Leu Ser Trp Asn Met Gly Phe Ala Ile Gly Gly 
385                 390                 395                 400 


Met Met Pro Thr Phe Val Asn Leu Ala Ser Gly Thr Val Glu His Ile 
                405                 410                 415     


Pro Tyr Thr Leu Met Tyr Phe Thr Ile Gly Ile Tyr Leu Val Tyr Ile 
            420                 425                 430         


Leu Gly Ser Leu Ile Ile Pro Glu Thr Lys Gly Asn Leu Lys 
        435                 440                 445     


<210>  18
<211>  1338
<212>  DNA
<213>  Aeribacillus pallidus

<400>  18
gtgaagaata tcgctaatac gagtaccgaa cgacctgtaa atgatgcttc agttaagaat     60

cgtcaaatgg tgcgagctac gattgcctcg ctcatagggt ggtcactcga tctttacgat    120

ttatttctgc tgctttttgt tgcgacgacc atagggaatt tgttttttcc cgccagcaat    180

caaacacttt ctttggctgc cgtgtatgct tcctttgccg ttacgctttt gatgcggcct    240

ttgggttccg ccattttcgg catttatgcg gataaaaacg ggagaaagaa agcgatgact    300

gtggcaatca ttggagcagg cttgtgcacg gcggctttcg gtctgttacc tacgatccac    360

caagttggag tggtcgctgc gatcgccttc ttgattttac gtttagttca aggagtgttt    420

gtcggcggag tggttgcttc cacccatacg ataggaacgg aatccgcatc gccaaaatat    480

cgggggttta tgtcgggatt gatcggtggt ggcggagcag gattgggagc actgtttgct    540

tctatttctt attcggttgt gacggcaatt tttccgggag aggcttttga tgtttgggga    600

tggcgtgtca tgtttttcac aggcattatc ggttccctct tcggcctttt catattccgg    660

tcccttgagg aatctcctct ctggaaacaa ttgaaagaag aaaatagtaa aggcgaagtg    720

tccgagtttc agaaagcacc gctgaagacg tttttcacta aatattacaa ggtattgctc    780

gtcaacctta tgatcgtcat cggtggtggc tccggttatt atctgactag tggatttatt    840

cctacatttt taaaggtagt taacaaagta tcagcctctg tttcgtcggg ggtactcatt    900

gcgacaagta ttatgaccat tgtagccgcc gttctcgtgg gacacctgag cgaggtcatc    960

ggcagaaaga aaacatttct gttaatcggt attctttgtc ttgtcggact tccgtatttt   1020

tatctgtcat tggcaaactc aactacgaca acgggcatct acttaaatgc tcttggactc   1080

atattcttgg ggaatgctgc atatgcaccg gtactcatct tcttgaacga acgttttccc   1140

acatcgatcc gttcaacagg taccggatta tcatggaaca tgggtttcgc cattggcggg   1200

atgatgccga cgtttgtgaa cttagccagt ggtacggtgg aacatattcc ttacacgctg   1260

atgtatttta ctatcggaat ttacttggtt tatatccttg gcagcctgat tattccggaa   1320

acaaaaggaa acctcaaa                                                 1338


<210>  19
<211>  1184
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI - NheI fragment with codon optimized B.kribbensis 
       YiaY

<400>  19
ggtacctagg aaaggaagat taacccatgg acgtggaatt cagcttccat ctgccgaccc     60

tgatcgagtt cggcttcggc aaggccagcc tgctgggcga gcgcctgctg aagctgggcg    120

tgggcaacgt gttcctggtg agcgacaagg gcgtggccag cgcaggcctg ctgcagaagc    180

tggaacagag cctgcagacc agcgacatcc acttcaagac ctacctggaa gtggaaccgg    240

acccgagcct ggaaaccatc gacctgggtg ccgaggcctt caacagcggc aagtacgact    300

gcatcgtggc cgtgggtggt ggcagcgcca tcgacaccgc caagggcatc cgcgtggtgg    360

caggcaacgg tggcagcatc ggcgacttcg caggcgtgga caagatcggc aaggcaccgc    420

agatcccgct gatcgccgtg ccgaccacct cgggcaccgg cagcgaagtg accatcttcg    480

gcgtgtacag cgactgggtg aagaacgtga aggtgaccgt gaccagccag tacatggcac    540

cgaccattgc cctggtggac ccggaactga ccatgcgcct gccacgcaag atgaccgcag    600

ccagcggcat cgacgccctg gcccacggca tcgagagcta cttcagcctg cgcagcacca    660

gcgccagccg tgccctgtcg ctggaagcca tcaacatcgt gggcaaccat ctgcgccaga    720

gcgtggcgaa cggcgaggac aaggaagcac gctgcggcat gagccacggc agcctgctgg    780

caggcatggc gttcaacaac ggcttcctgg gcctggccca tgccatcggc agcgcactga    840

gcggtcactg ccacgtgccg cacggcgtgg ccatcggcct gctgctgccg cacgtggtgg    900

aattcaacag cagcgagtgc ccagaccagg cagccgagat cgccaagatc ctgggcgtga    960

aggccgagga cgaacgccag ctggccgaac aggccagcca cgccgtgggc gacctggtga   1020

aggacatcgg cctgccgacc cgtctgcgcg acatgaacgt gccggaagag aagctggccg   1080

acattgcacg cgacagcttc cagagcggca tgatgaagtt caacccacgt cgtgccagcg   1140

agagcgaggt gctggaactg ctgcaccgcg tgtactgagc tagc                    1184


<210>  20
<211>  1359
<212>  DNA
<213>  Artificial

<220>
<223>  EcoRI-NheI PCR fragment with A.pallidus proP coding sequence

<400>  20
gccgaattca tgaagaatat cgctaatacg agtaccgaac gacctgtaaa tgatgcttca     60

gttaagaatc gtcaaatggt gcgagctacg attgcctcgc tcatagggtg gtcactcgat    120

ctttacgatt tatttctgct gctttttgtt gcgacgacca tagggaattt gttttttccc    180

gccagcaatc aaacactttc tttggctgcc gtgtatgctt cctttgccgt tacgcttttg    240

atgcggcctt tgggttccgc cattttcggc atttatgcgg ataaaaacgg gagaaagaaa    300

gcgatgactg tggcaatcat tggagcaggc ttgtgcacgg cggctttcgg tctgttacct    360

acgatccacc aagttggagt ggtcgctgcg atcgccttct tgattttacg tttagttcaa    420

ggagtgtttg tcggcggagt ggttgcttcc acccatacga taggaacgga atccgcatcg    480

ccaaaatatc gggggtttat gtcgggattg atcggtggtg gcggagcagg attgggagca    540

ctgtttgctt ctatttctta ttcggttgtg acggcaattt ttccgggaga ggcttttgat    600

gtttggggat ggcgtgtcat gtttttcaca ggcattatcg gttccctctt cggccttttc    660

atattccggt cccttgagga atctcctctc tggaaacaat tgaaagaaga aaatagtaaa    720

ggcgaagtgt ccgagtttca gaaagcaccg ctgaagacgt ttttcactaa atattacaag    780

gtattgctcg tcaaccttat gatcgtcatc ggtggtggct ccggttatta tctgactagt    840

ggatttattc ctacattttt aaaggtagtt aacaaagtat cagcctctgt ttcgtcgggg    900

gtactcattg cgacaagtat tatgaccatt gtagccgccg ttctcgtggg acacctgagc    960

gaggtcatcg gcagaaagaa aacatttctg ttaatcggta ttctttgtct tgtcggactt   1020

ccgtattttt atctgtcatt ggcaaactca actacgacaa cgggcatcta cttaaatgct   1080

cttggactca tattcttggg gaatgctgca tatgcaccgg tactcatctt cttgaacgaa   1140

cgttttccca catcgatccg ttcaacaggt accggattat catggaacat gggtttcgcc   1200

attggcggga tgatgccgac gtttgtgaac ttagccagtg gtacggtgga acatattcct   1260

tacacgctga tgtattttac tatcggaatt tacttggttt atatccttgg cagcctgatt   1320

attccggaaa caaaaggaaa cctcaaataa gctagcggc                          1359


<210>  21
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  ribosome binding site

<400>  21
taggaaagga agattaaccc                                                 20


<210>  22
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer proP(f)

<400>  22
gccgaattca tgaagaatat cgctaatacg                                      30


<210>  23
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer proP(r)

<400>  23
gccgctagct tatttgaggt ttccttttgt ttcc                                 34


<210>  24
<211>  500
<212>  PRT
<213>  Cupriavidus basilensis

<400>  24

Met Asn Ala Gln His Trp Ile Ala Gly Ala Trp Thr Gly Glu Pro Ser 
1               5                   10                  15      


Ala Asp Ser Val Asn Pro Ala Asp Gly Thr Leu Ile Gly Gln Phe Ala 
            20                  25                  30          


Asp Gly Gly Thr Trp Gln Ala Glu Ala Ala Ile Ala Ala Ala Arg His 
        35                  40                  45              


Val Phe Glu Arg Thr Thr Trp Gly Gln Asp Ala Arg Leu Arg Gln Asp 
    50                  55                  60                  


Val Leu Leu Ala Trp Ala Gly Ala Leu Glu Ala Glu Arg Glu Arg Leu 
65                  70                  75                  80  


Ala Ser Leu Leu Thr Ala Glu Asn Gly Lys Pro Val Ala Gln Ala Arg 
                85                  90                  95      


Gly Glu Val Gly Ala Ala Ile Ser Glu Val Arg Tyr Tyr Ala Gly Leu 
            100                 105                 110         


Ala Arg His Ile Pro Gly His Val Leu Glu Pro Glu Pro Gly Thr Ile 
        115                 120                 125             


Ser Thr Ile Leu Arg Glu Pro Ala Gly Val Ala Ala Ile Ile Val Pro 
    130                 135                 140                 


Trp Asn Ala Pro Ala Val Leu Leu Val Arg Ser Leu Ala Pro Ala Leu 
145                 150                 155                 160 


Ala Ala Gly Cys Thr Ala Val Val Lys Ser Ala Ala Gln Thr Thr Leu 
                165                 170                 175     


Phe Thr Ala Ala Met Leu Arg Leu Phe Glu Arg Thr Ala Leu Pro Ala 
            180                 185                 190         


Gly Ala Val Asn Leu Val Cys Glu Thr Gly Tyr Ala Ala Ala Asp His 
        195                 200                 205             


Leu Val Arg Ser Arg Asp Val Asp Val Val Ser Phe Thr Gly Ser Thr 
    210                 215                 220                 


Ala Thr Gly Lys Lys Ile Met Ile Ala Ala Ala Asp Ser Val Lys Lys 
225                 230                 235                 240 


Leu Ser Leu Glu Leu Gly Gly Lys Ser Cys Cys Leu Val Phe Asp Asp 
                245                 250                 255     


Val Asp Ala Gln Ala Val Ala Lys Arg Leu Ala Leu Ala Ala Thr Val 
            260                 265                 270         


Ile Ser Gly Gln Gln Cys Thr Ala Ala Arg Arg Val Leu Val His Glu 
        275                 280                 285             


Ala Ile Ala Pro Gln Met Arg Arg His Leu Thr Glu Ala Leu Ala Ala 
    290                 295                 300                 


Leu Arg Leu Gly Pro Gly Ile Glu Pro Asp Thr Gln Ile Gly Pro Leu 
305                 310                 315                 320 


Ile Asp His Pro Thr Arg Ala Met Val Ser Ala Gln Val Glu Arg Ala 
                325                 330                 335     


Cys Asp Glu Ala Asp Thr Val Leu Leu Arg Gly Thr Met Pro Gly Gly 
            340                 345                 350         


Ala Leu Ala Arg Gly Ala Phe Leu Ser Pro Thr Leu Val Glu His Ser 
        355                 360                 365             


Asp Pro Gly Ala Phe Phe Cys Gln Glu Glu Ile Phe Gly Pro Phe Val 
    370                 375                 380                 


Thr Phe Glu Thr Phe Ala Thr Glu Asp Glu Ala Leu Ala Lys Ala Asn 
385                 390                 395                 400 


Asn Thr Val Phe Gly Leu Ser Ala Ser Val Trp Thr His His Gly Glu 
                405                 410                 415     


Arg Ala Ile Arg Leu Ala Arg Ala Leu Arg Asn Gly Thr Val Trp Val 
            420                 425                 430         


Asn Asp His Asn Arg Leu Phe Ala Glu Ala Glu Thr Gly Gly Tyr Arg 
        435                 440                 445             


Gln Ser Gly Leu Gly Arg Leu His Gly Tyr Asp Ala Leu Ala Asp Phe 
    450                 455                 460                 


Thr Glu Leu Lys His Ile Cys Ile Gln Ala Gly Leu Pro Lys Gly Met 
465                 470                 475                 480 


Ser Gln Ala Gly Cys Arg Leu Ser Gly Val Ala Ala Arg Glu Arg Met 
                485                 490                 495     


Gly Val Ser Val 
            500 


<210>  25
<211>  479
<212>  PRT
<213>  Burkholderia sp. CCGE1002

<400>  25

Met Asn Ala Arg His Trp Ile Ala Gly Glu Trp Thr Gly Thr Pro Asn 
1               5                   10                  15      


Ile Asp Ser Ile Asp Pro Ala Thr Gly Asp Ala Ile Gly Arg Phe Ala 
            20                  25                  30          


Asp Gly Gly Ser Ser Glu Ala Asp Ala Ala Ile Ala Ala Ala Arg His 
        35                  40                  45              


Ala Phe Asp Arg Thr Thr Trp Ala Gln Asp Ala Arg Leu Arg Gln Asp 
    50                  55                  60                  


Val Leu Leu Gly Trp Ala Ser Ala Leu Glu Ala Glu Arg Asp Met Leu 
65                  70                  75                  80  


Ala Thr Leu Leu Thr Arg Glu Asn Gly Lys Ala Ile Ala Gln Ser Arg 
                85                  90                  95      


Asp Glu Ile Ala Gly Ala Ile Ser Glu Val Arg Tyr Tyr Ala Gly Leu 
            100                 105                 110         


Ala Arg His Ile Ala Gly His Val Leu Glu Pro Glu Pro Gly Thr Ile 
        115                 120                 125             


Ser Thr Met Leu Arg Glu Ala Ala Gly Val Ala Ala Ile Ile Val Pro 
    130                 135                 140                 


Trp Asn Ala Pro Ala Val Leu Leu Val Arg Ser Leu Ala Pro Ala Leu 
145                 150                 155                 160 


Ala Ala Gly Cys Thr Val Ile Val Lys Pro Ala Ala Gln Thr Ser Leu 
                165                 170                 175     


Leu Thr Ala Ala Met Leu Arg Cys Phe Glu His Thr Ala Leu Pro Glu 
            180                 185                 190         


Gly Ala Val Asn Leu Val Asn Glu Arg Gly Tyr Ala Ala Ser Gln Arg 
        195                 200                 205             


Leu Val Asp Ser His Gly Val Asp Val Val Ser Phe Thr Gly Ser Thr 
    210                 215                 220                 


Ala Thr Gly Lys Lys Ile Met Ala Ala Ala Ala Asp Ser Met Lys Lys 
225                 230                 235                 240 


Leu Ser Leu Glu Leu Gly Gly Lys Ser Cys Cys Val Val Phe Asp Asp 
                245                 250                 255     


Ala Asp Val Ala Ala Ile Ala Pro Arg Leu Ala Arg Ala Ala Thr Ile 
            260                 265                 270         


Ile Ser Gly Gln Gln Cys Thr Ala Ala Arg Arg Val Leu Val His Ala 
        275                 280                 285             


Ser Arg Ala Ala Gln Met Arg Glu Gln Leu Ala Ser Ala Leu Ala Ser 
    290                 295                 300                 


Leu Arg Val Gly Pro Gly Ile Asp Pro Ala Thr Asp Ile Gly Ala Leu 
305                 310                 315                 320 


Ile Asp Gly Thr Thr Arg Asp Ala Val Ala Arg Thr Ile Glu Arg Ala 
                325                 330                 335     


Cys Gly Thr Ala Glu Arg Val Leu Leu Arg Gly Thr Cys Ser Gly His 
            340                 345                 350         


Ala Phe Leu Ser Pro Thr Leu Val Glu His Asp Asp Pro Lys Ala Phe 
        355                 360                 365             


Phe Cys Gln Asp Glu Ile Phe Gly Pro Phe Val Thr Leu Glu Val Phe 
    370                 375                 380                 


Glu Asn Glu Met Glu Ala Ile Glu Lys Ala Asn Asp Thr Val Phe Gly 
385                 390                 395                 400 


Leu Ser Ala Ser Val Trp Thr His Asp Gly Ala Arg Ala Leu Arg Val 
                405                 410                 415     


Ala Arg Ala Leu Arg Asn Gly Thr Val Trp Ile Asn Asp His Asn Lys 
            420                 425                 430         


Leu Phe Ala Glu Ala Glu Thr Gly Gly Tyr Arg Gln Ser Gly Leu Gly 
        435                 440                 445             


Arg Leu His Gly Tyr Asp Ala Leu Ala Asp Phe Thr Glu Leu Lys His 
    450                 455                 460                 


Ile Cys Met Pro Ala Gly Val Ala Glu Gly Ile Ala Pro Leu Arg 
465                 470                 475                 


<210>  26
<211>  483
<212>  PRT
<213>  Burkholderia graminis C4D1M

<400>  26

Met Glu Arg Asp Ala Met Asn Trp Ile Ala Gly Glu Trp Ala Gly Lys 
1               5                   10                  15      


Pro Val Leu Ala Ser Ser Asp Pro Ser Asn Gly Glu Thr Leu Gly Arg 
            20                  25                  30          


Phe Val Ser Ser Asn Thr Gln Asp Ala Asp Ala Ala Val Ser Ala Ala 
        35                  40                  45              


Arg His Thr Phe Asp His Thr Thr Trp Ala Gln Asp Ala Arg Arg Arg 
    50                  55                  60                  


Gln Asp Val Leu Leu Arg Trp Ala Gln Ala Leu Glu Leu Ser Val Glu 
65                  70                  75                  80  


Pro Leu Ala Glu Leu Leu Thr His Glu Asn Gly Lys Thr Ile Gly Gln 
                85                  90                  95      


Ala Arg Gly Glu Met Arg Ala Ala Ile Ser Glu Val Arg Tyr Tyr Ala 
            100                 105                 110         


Gly Leu Ala Arg His Ile Ala Gly His Val Ile Glu Pro Glu Pro Gly 
        115                 120                 125             


Thr Ile Ser Thr Met Leu Arg Glu Ala Ala Gly Val Ala Ala Ile Ile 
    130                 135                 140                 


Val Pro Trp Asn Ala Pro Ala Val Leu Leu Val Arg Ser Leu Ala Pro 
145                 150                 155                 160 


Ala Leu Ala Ala Gly Cys Thr Ala Ile Val Lys Pro Ala Ala Gln Thr 
                165                 170                 175     


Ser Leu Ile Thr Ala Ala Met Ile Arg Cys Leu Asp Gln Pro Ala Leu 
            180                 185                 190         


Pro Ala Gly Ala Val Asn Leu Leu Leu Glu Asn Gly Ala Glu Ala Ala 
        195                 200                 205             


Ala Arg Leu Val Glu Ser Ala Asp Val Asp Val Ile Ser Phe Thr Gly 
    210                 215                 220                 


Ser Thr Glu Val Gly Lys Arg Ile Met Arg Ala Ala Ala Asp Ser Met 
225                 230                 235                 240 


Lys Arg Leu Ser Leu Glu Leu Gly Gly Lys Ser Cys Cys Leu Val Phe 
                245                 250                 255     


Glu Asp Ser Asp Val Lys Ala Ile Ala Pro Arg Leu Ala Arg Ala Ala 
            260                 265                 270         


Thr Ile Ile Ser Gly Gln Gln Cys Thr Ala Ala Arg Arg Ile Leu Val 
        275                 280                 285             


His Val Ser Lys Ala Asp Gln Met Arg Asp Glu Leu Val Lys Ala Leu 
    290                 295                 300                 


Ala Ser Leu Lys Val Gly Pro Gly Ile Asp Pro Ala Ser Asp Ile Gly 
305                 310                 315                 320 


Ala Leu Ile Asp Ala Ala Ser Arg Asp Ala Val Gln Thr Thr Val Glu 
                325                 330                 335     


Arg Ala Cys Asp Leu Ala Asp Arg Val Leu Leu Arg Gly Thr Ser Ser 
            340                 345                 350         


Gly Pro Gly Ala Phe Leu Ser Pro Thr Leu Val Glu His Gly Glu Pro 
        355                 360                 365             


His Ala Phe Phe Cys Gln Asp Glu Ile Phe Gly Pro Phe Val Thr Leu 
    370                 375                 380                 


Glu Thr Phe Val Thr Glu Lys Glu Ala Val Glu Lys Ala Asn Asn Thr 
385                 390                 395                 400 


Val Phe Gly Leu Ser Ala Ser Val Trp Thr His Asp Ser Ala Arg Ala 
                405                 410                 415     


Phe Arg Ile Ala Arg Ala Leu Arg Asp Gly Thr Val Trp Ile Asn Asp 
            420                 425                 430         


His Asn Arg Leu Phe Ala Glu Ala Glu Thr Gly Gly Tyr Arg Gln Ser 
        435                 440                 445             


Gly Leu Gly Arg Leu His Gly Tyr Asp Ala Leu Ala Asp Phe Thr Glu 
    450                 455                 460                 


Ile Lys His Ile Cys Val Gly Ala Gly Val Leu Glu Gly Ile Glu Val 
465                 470                 475                 480 


Leu Gly Ser 
            


<210>  27
<211>  483
<212>  PRT
<213>  Azospirillum sp. B510

<400>  27

Met Thr Asn Leu Asp Ser Arg His Trp Ile Asp Gly Ala Trp Val Pro 
1               5                   10                  15      


Gly Thr Asp Arg Phe Ala Ser Ile Asn Pro Ala Asp Gly Ser Val Leu 
            20                  25                  30          


Gly His Ala Ala Asp Gly Gly Arg Ala Glu Ala Glu Ala Ala Ile Ala 
        35                  40                  45              


Ala Ala His Ala Ala Phe Asn Arg Pro Asp Trp Ala Gln Asn Pro Arg 
    50                  55                  60                  


Leu Arg Gln Ser Ile Leu Leu Gly Trp Ala Asp Arg Leu Asp Thr Gln 
65                  70                  75                  80  


Ala Glu Asp Leu Ala Arg Leu Leu Thr Leu Glu Asn Gly Lys Ala Ile 
                85                  90                  95      


Ala Gln Ser Arg Gly Glu Ile Ala Gly Ala Ile Ser Glu Ile Arg Tyr 
            100                 105                 110         


Tyr Gly Gly Leu Ala Arg His Val Pro Gly His Val Leu Glu Val Glu 
        115                 120                 125             


Pro Gly Val Leu Ser Thr Met Leu Arg Glu Pro Ala Gly Val Ala Ala 
    130                 135                 140                 


Leu Ile Ile Pro Trp Asn Ala Pro Ala Val Leu Leu Ala Arg Ala Ile 
145                 150                 155                 160 


Gly Pro Ala Leu Ala Cys Gly Cys Thr Val Val Val Lys Pro Ala Ala 
                165                 170                 175     


Gln Thr Thr Leu Leu Thr Ala Ala Phe Leu Arg Ala Leu Ser Glu Val 
            180                 185                 190         


Pro Ser Leu Pro Arg Gly Val Cys Asn Met Ile Ser Glu Thr Gly His 
        195                 200                 205             


Ala Ala Ala Ala Arg Leu Val Asp Ser Pro Leu Val Asp Val Val Ser 
    210                 215                 220                 


Phe Thr Gly Ser Thr Ala Thr Gly Lys Arg Ile Met Val Ala Ala Ala 
225                 230                 235                 240 


Asp Thr Met Lys Lys Leu Ser Leu Glu Leu Gly Gly Lys Ser Cys Cys 
                245                 250                 255     


Leu Val Phe Pro Asp Ala Asp Pro Ala Glu Thr Ala Ala Arg Ile Ala 
            260                 265                 270         


Thr Ala Ala Thr Ile Ile Ser Gly Gln Gln Cys Thr Ala Ala Arg Arg 
        275                 280                 285             


Val Leu Val His Ala Ser Ala Phe Asp Ala Met Lys Thr His Leu Arg 
    290                 295                 300                 


Ala Ala Leu Ala Ala Met Thr Val Gly Asn Gly Leu Asp Pro Ala Ile 
305                 310                 315                 320 


Arg Met Gly Pro Leu Ile Asp Arg Pro Ala Arg Asp Gln Val Gln Thr 
                325                 330                 335     


Gln Val Glu Arg Ala Phe Asp Ala Cys Asp Glu Val Leu Leu Arg Gly 
            340                 345                 350         


Gly Val Pro Thr Asp Ser Pro Ala Ala Ala Ser Phe Leu Thr Pro Ser 
        355                 360                 365             


Leu Val Ala His Asp Asp Pro Ser Ala Phe Phe Cys Gln Asp Glu Ile 
    370                 375                 380                 


Phe Gly Pro Phe Val Val Leu Glu Arg Phe Glu Thr Glu Ala Glu Ala 
385                 390                 395                 400 


Val Ala Lys Ala Asn Asn Thr Val Phe Gly Leu Ser Ala Ser Val Trp 
                405                 410                 415     


Thr Arg Asp Gly Ala Arg Ala Leu Arg Met Ala Arg Ala Leu Arg Asn 
            420                 425                 430         


Gly Thr Val Trp Ile Asn Asp His Asn Arg Leu Phe Ala Glu Ala Glu 
        435                 440                 445             


Thr Gly Gly Tyr Arg Gln Ser Gly Leu Gly Arg Leu His Gly Tyr Asp 
    450                 455                 460                 


Ala Phe Ala Asp Phe Thr Glu Leu Lys His Val Cys Gln Thr Val Gly 
465                 470                 475                 480 


Thr Ile Gly 
            


<210>  28
<211>  480
<212>  PRT
<213>  Pseudomonas putida

<400>  28

Met Gln Ser Gln His Tyr Ile Asp Gly Gln Trp Thr Ser Thr Asp Arg 
1               5                   10                  15      


Trp Thr Asp Ser Leu Asp Pro Ala Ser Gly Glu Leu Ile Gly Cys Phe 
            20                  25                  30          


Ala Asp Gly Gly Glu Ala Glu Ala Glu Ala Ala Val Ala Ala Ala Ala 
        35                  40                  45              


Arg Ala Phe Asn Asp Pro Gln Trp Ala Gln Asn Pro Arg Leu Arg Gln 
    50                  55                  60                  


Gln Leu Leu Leu Glu Trp Ala Ala Gly Leu Lys Ala Arg Gln Glu Gln 
65                  70                  75                  80  


Leu Ala Gln Leu Leu Thr Arg Glu Asn Gly Lys Ala Leu Ala Gln Ser 
                85                  90                  95      


Arg Gly Glu Ile Gly Gly Ala Ile Ser Glu Ile Leu Tyr Tyr Ala Gly 
            100                 105                 110         


Leu Ala Arg His Asn Pro Gly His Met Leu Glu Val Ala Pro Gly Glu 
        115                 120                 125             


Phe Ser Ser Met Leu Arg Glu Pro Ala Gly Val Ala Gly Leu Ile Ile 
    130                 135                 140                 


Pro Trp Asn Ala Pro Ala Val Leu Leu Val Arg Ala Leu Ala Pro Ala 
145                 150                 155                 160 


Ile Ala Ala Gly Cys Thr Val Val Ile Lys Pro Ala Pro Gln Thr Ala 
                165                 170                 175     


Leu Phe Asn Ala Ala Met Leu Glu Pro Leu Phe Ala Leu Pro Gly Leu 
            180                 185                 190         


Pro Ala Gly Ala Val Asn Leu Phe Ala Glu Ser Gly His Ala Gly Ala 
        195                 200                 205             


Ala His Leu Val Ala Ser Pro Arg Val Asp Val Leu Ser Phe Thr Gly 
    210                 215                 220                 


Ser Thr Ala Thr Gly Gln Arg Ile Met Arg Asp Cys Ala Ala Thr Met 
225                 230                 235                 240 


Lys Lys Leu Ser Leu Glu Leu Gly Gly Lys Ser Cys Cys Leu Val Phe 
                245                 250                 255     


Glu Asp Ala Asp Ile Ala Ala Ile Ala Pro Lys Leu Ala Ala Ala Ala 
            260                 265                 270         


Thr Ile Ile Ser Gly Gln Gln Cys Thr Ala Ala Arg Arg Val Leu Val 
        275                 280                 285             


His Ala Ser Arg Phe Ala Glu Met Lys Thr Ala Leu Ser Ala Ala Leu 
    290                 295                 300                 


Gly Gln Ile Arg Leu Gly Asn Gly Leu Asp Pro Ala Asn Asn Met Gly 
305                 310                 315                 320 


Pro Leu Ile Asp Trp His Ser Arg Asp Ser Val Glu Arg Arg Ile Gly 
                325                 330                 335     


Glu Ala Leu Asp Ser Cys Asp Glu Val Leu Leu Ala Gly Gly Arg Pro 
            340                 345                 350         


Gln Gly Glu Leu Ser Lys Gly Ala Phe Leu Ala Pro Ser Leu Ile Ala 
        355                 360                 365             


His Arg Asp Ser Ser Ala Phe Phe Cys Gln Glu Glu Ile Phe Gly Pro 
    370                 375                 380                 


Leu Leu Val Leu Glu Ser Phe Glu Asp Glu Thr Glu Ala Val Ala Arg 
385                 390                 395                 400 


Ala Asn His Thr Glu Phe Gly Leu Ser Ala Ser Val Trp Thr Asp Gln 
                405                 410                 415     


Gly Ala Arg Ala Trp Arg Val Ala Arg Ala Leu Arg Asn Gly Thr Val 
            420                 425                 430         


Trp Leu Asn Asp His Asn Arg Leu Phe Ala Glu Ala Glu Thr Gly Gly 
        435                 440                 445             


Tyr Arg Lys Ser Gly Leu Gly Arg Leu His Gly Val Asp Ala Leu Leu 
    450                 455                 460                 


Asp Phe Ser Glu Leu Lys His Ile Tyr Gln Asn Val Gly Thr Leu Gly 
465                 470                 475                 480 


<210>  29
<211>  486
<212>  PRT
<213>  Rhodopseudomonas palustris

<400>  29

Met Gly Met Thr Ala Leu His Ala Asp Asn Leu Ile Asp Gly Ala Trp 
1               5                   10                  15      


Gln Pro Ala Gln Ser Gly Ala Thr Ala Pro Ser Leu Asp Pro Ser Ser 
            20                  25                  30          


Gly Gly Thr Ile Gly Gly Phe Ala Ala Gly Gly Ala Ala Asp Ala Gln 
        35                  40                  45              


Ala Ala Val Ala Ala Ala Arg Arg Ala Phe Glu Arg Pro Glu Trp Ser 
    50                  55                  60                  


Gln Asn Pro Arg Ala Arg Gln Met Val Met Leu Arg Trp Ala Asp Arg 
65                  70                  75                  80  


Met Glu Ala Gln Ala Asp Gln Leu Ala Arg Leu Leu Thr Leu Glu Asn 
                85                  90                  95      


Gly Lys Pro Leu Pro Gln Ser Arg Gly Glu Ile Ala Gly Ser Val Ser 
            100                 105                 110         


Glu Ile Arg Tyr Tyr Ala Gly Leu Thr Arg Tyr Ile Pro Gly His Val 
        115                 120                 125             


Phe Glu Val Glu Pro Gly Ser Phe Ser Thr Leu Leu Lys Glu Pro Ala 
    130                 135                 140                 


Gly Val Ala Gly Leu Ile Ile Pro Trp Asn Ala Pro Ala Val Leu Leu 
145                 150                 155                 160 


Ile Arg Ala Leu Thr Pro Ala Leu Ala Ala Gly Cys Thr Val Val Ile 
                165                 170                 175     


Lys Pro Ala Pro Gln Thr Ala Gln Ile Thr Ala Ala Ile Ile Lys Cys 
            180                 185                 190         


Leu His Glu Val Asp Gly Leu Pro Arg Gly Val Val Asn Leu Val Ser 
        195                 200                 205             


Glu Gln Gly His Gln Val Ala Glu His Leu Val Thr Ser Asn Asp Val 
    210                 215                 220                 


Asp Val Ile Ser Phe Thr Gly Ser Asn Ala Thr Gly Ala Arg Ile Met 
225                 230                 235                 240 


Ala Ala Ala Ala Pro Thr Met Lys Lys Leu Ser Leu Glu Leu Gly Gly 
                245                 250                 255     


Lys Ser Ala Cys Leu Val Phe Asp Asp Ala Asp Ile Ala Asp Val Ala 
            260                 265                 270         


Pro Lys Leu Ala Ala Ala Ala Thr Ile Ile Ala Gly Gln Gln Cys Thr 
        275                 280                 285             


Ala Ala Arg Arg Val Leu Val His Ala Ser Arg Tyr Asp Glu Met Lys 
    290                 295                 300                 


Ala Ala Leu Lys Ala Ala Leu Ala Asn Ile Arg Ile Ala Pro Gly Ser 
305                 310                 315                 320 


Ala Ala Gly Ala Glu Met Gly Pro Leu Ile Asp Ala Ala Ser Leu Ala 
                325                 330                 335     


Ala Val Ala Lys Arg Ala Asp Glu Ala Met Gln Ala Ala Asp Glu Val 
            340                 345                 350         


Val Leu Arg Gly Gly Arg Pro Ala Gly Asp Leu Ala Asn Gly Tyr Phe 
        355                 360                 365             


Leu Ser Pro Thr Leu Val Ala His Arg Asp Thr Ser Ala Phe Phe Val 
    370                 375                 380                 


Gln Glu Glu Ile Phe Gly Pro Leu Val Val Leu Glu Lys Phe Glu Asp 
385                 390                 395                 400 


Glu Lys Glu Ala Val Ala Arg Ala Asn His Ser Asp Tyr Gly Leu Ser 
                405                 410                 415     


Ala Ser Val Trp Thr His Asp Gly Ala Arg Ala Met Arg Val Ala Arg 
            420                 425                 430         


Ala Leu Arg Asn Gly Thr Val Trp Ile Asn Asp His Asn Lys Leu Phe 
        435                 440                 445             


Ala Glu Ala Glu Thr Gly Gly Tyr Arg Arg Ser Gly Leu Gly Arg Leu 
    450                 455                 460                 


His Gly Tyr Asp Ala Leu Ile Asp Phe Leu Glu Ile Lys His Val Tyr 
465                 470                 475                 480 


Gln Ser Cys Gly Val Val 
                485     


<210>  30
<211>  485
<212>  PRT
<213>  Dinoroseobacter shibae DFL 12

<400>  30

Met Thr Thr Thr Asp Leu Ile Ala Arg His Met Ile Gly Gly Ser Tyr 
1               5                   10                  15      


Ser Asp Ala Gly Asp Lys Ile Ala Ser Ile Asn Pro Ala Thr Gly Ala 
            20                  25                  30          


Val Val Gly His Val Arg Ala Asp Gly Ala Ala Gln Ala Thr Ala Ala 
        35                  40                  45              


Ile Ala Ala Ala Arg Ala Ala Phe Asp Thr Thr Leu Trp Pro Gln Ser 
    50                  55                  60                  


Pro Arg Asp Arg Gln Met Ala Leu Leu Arg Trp Ala Asp Ala Leu Glu 
65                  70                  75                  80  


Ala Asp Leu Ala Arg Leu Ala Glu Leu Leu Thr Leu Thr Asn Gly Lys 
                85                  90                  95      


Pro Leu Gly Ala Ser Lys Gly Glu Leu Gly Ala Ala Ile Ser Glu Ile 
            100                 105                 110         


Arg Tyr Tyr Ala Gly Leu Thr Arg His Asn Pro Gly His Ala Met Glu 
        115                 120                 125             


Val Ala Pro Gly Glu Leu Ser Val Met Leu Arg Glu Pro Ala Gly Val 
    130                 135                 140                 


Ala Gly Ile Ile Val Pro Trp Asn Ala Pro Ala Val Leu Leu Ile Arg 
145                 150                 155                 160 


Ser Leu Ala Pro Ala Leu Ala Val Gly Cys Thr Thr Val Thr Lys Pro 
                165                 170                 175     


Ala Pro Gln Thr Ala Leu Phe Thr Ala Ala Cys Met Ala Pro Leu Phe 
            180                 185                 190         


Glu Asp Ala Ala Ile Pro Ala Gly Val Val Asn Val Val Phe Glu Val 
        195                 200                 205             


Gly His Asp Ala Ala Gln Thr Leu Val Thr Ser Pro Asp Val Asp Val 
    210                 215                 220                 


Ile Ser Phe Thr Gly Ser Asn Ala Val Gly Gln Arg Ile Met Ala Asp 
225                 230                 235                 240 


Ala Ala Pro Thr Met Lys Lys Leu Ser Leu Glu Leu Gly Gly Lys Ser 
                245                 250                 255     


Cys Cys Ile Val Leu Asp Asp Ala Asp Ile Gly Val Val Ala Pro Lys 
            260                 265                 270         


Leu Ala Ala Ala Ala Thr Ile Ile Ser Gly Gln Gln Cys Thr Ala Ala 
        275                 280                 285             


Arg Arg Val Leu Val His Glu Ser Arg Leu Asp Glu Ala Lys Ser Ala 
    290                 295                 300                 


Leu Ser Ala Ala Leu Gln Ala Val Ser Ile Gly Asp Gly Met Ser Asp 
305                 310                 315                 320 


Gly Thr Ala Met Gly Pro Leu Ile Asp Ile Gln Ser Arg Asp Arg Val 
                325                 330                 335     


Met Arg Asp Cys Gly Thr Val Tyr Asp Thr Ala Asp Glu Val Val Leu 
            340                 345                 350         


Arg Gly Gly Pro Leu Asp Gly Pro Lys Gly Ser Ala Phe Met Ser Pro 
        355                 360                 365             


Ala Leu Val Val His Ser Asp Pro Asn Ala Ser Phe Val Gln Asp Glu 
    370                 375                 380                 


Ile Phe Gly Pro Leu Val Val Leu Glu Thr Phe Arg Asp Glu Ala Asp 
385                 390                 395                 400 


Ala Val Ala Lys Ala Asn Asn Thr Val Tyr Gly Leu Ser Ala Ser Ile 
                405                 410                 415     


Trp Thr His Arg Gly Asp Ala Ser Trp Arg Leu Ala Arg Ala Leu Arg 
            420                 425                 430         


Asn Gly Thr Val Trp Ile Asn Asp His Asn Arg Leu Phe Ala Glu Ala 
        435                 440                 445             


Glu Thr Gly Gly Tyr Arg Arg Ser Gly Leu Gly Arg Leu His Gly Phe 
    450                 455                 460                 


Asp Gly Leu Leu Asp Phe Cys Glu Leu Lys His Val Tyr Gln Asn Val 
465                 470                 475                 480 


Gly Val Val Gly His 
                485 


<210>  31
<211>  447
<212>  PRT
<213>  Cupriavidus basilensis

<400>  31

Met Glu Ala Val Ala Lys Lys Arg Thr Glu Thr Ile Ser Glu Ala Leu 
1               5                   10                  15      


Pro Ala Ala Thr Asn Arg Gln Val Phe Gly Ala Val Thr Ala Ser Cys 
            20                  25                  30          


Met Gly Trp Ala Leu Asp Leu Phe Asp Leu Phe Ile Leu Leu Phe Val 
        35                  40                  45              


Ala Pro Val Ile Gly Arg Leu Phe Phe Pro Ser Glu His Ala Met Leu 
    50                  55                  60                  


Ser Leu Ala Ala Val Tyr Ala Ser Phe Ala Val Thr Leu Leu Met Arg 
65                  70                  75                  80  


Pro Leu Gly Ser Ala Ile Phe Gly Thr Tyr Ala Asp Arg His Gly Arg 
                85                  90                  95      


Lys Gly Ala Met Val Val Ala Val Thr Gly Val Gly Leu Ser Thr Ala 
            100                 105                 110         


Ala Phe Gly Leu Leu Pro Thr Val Gly Gln Val Gly Leu Leu Ala Pro 
        115                 120                 125             


Ala Leu Phe Ile Leu Leu Arg Leu Val Gln Gly Ile Phe Val Gly Gly 
    130                 135                 140                 


Val Val Ala Ser Thr His Thr Ile Gly Thr Glu Ser Val Pro Pro Ser 
145                 150                 155                 160 


Trp Arg Gly Ala Val Ser Gly Leu Val Gly Gly Gly Gly Ala Gly Ile 
                165                 170                 175     


Gly Ala Leu Leu Ala Ser Ile Thr Tyr Met Ala Met Thr Ala Leu Phe 
            180                 185                 190         


Pro Gly Glu Ala Phe Asp Ala Trp Gly Trp Arg Cys Met Phe Phe Ser 
        195                 200                 205             


Gly Ile Ile Ser Ser Val Leu Gly Leu Phe Ile Phe Asn Ser Leu Glu 
    210                 215                 220                 


Glu Ser Pro Leu Trp Lys Gln Leu Gln Ala Ala Lys Gly His Ala Ala 
225                 230                 235                 240 


Pro Val Glu Asn Pro Leu Arg Val Ile Phe Ser Arg Gln Tyr Arg Gly 
                245                 250                 255     


Val Leu Phe Val Asn Ile Leu Leu Thr Val Gly Gly Gly Ser Ala Tyr 
            260                 265                 270         


Tyr Leu Thr Ser Gly Tyr Leu Pro Thr Phe Leu Lys Val Val Val Lys 
        275                 280                 285             


Ala Pro Ala Gly Ala Ser Ala Ala Ile Leu Met Ala Ser Ser Val Gly 
    290                 295                 300                 


Val Ile Val Ala Ser Ile Ile Ala Gly His Leu Ser Thr Leu Ile Gly 
305                 310                 315                 320 


Arg Lys Arg Ala Phe Leu Leu Ile Gly Ala Leu Asn Val Val Leu Leu 
                325                 330                 335     


Pro Leu Ile Tyr Gln Arg Met Pro Ala Ala Pro Asp Val Thr Thr Leu 
            340                 345                 350         


Gly Ile Tyr Ala Val Ala Leu Ala Met Leu Gly Ser Thr Gly Phe Ala 
        355                 360                 365             


Pro Ile Leu Ile Phe Leu Asn Glu Arg Phe Pro Thr Ser Ile Arg Ala 
    370                 375                 380                 


Thr Gly Thr Gly Leu Ser Trp Asn Ile Gly Phe Ala Ile Gly Gly Met 
385                 390                 395                 400 


Met Pro Thr Phe Ala Ser Leu Cys Ala Ser Thr Pro Ala Asp Leu Pro 
                405                 410                 415     


Lys Val Leu Gly Ile Phe Val Ala Val Val Thr Ala Ile Tyr Leu Ala 
            420                 425                 430         


Gly Ala Ala Ile Val Pro Glu Thr Ala Gly Arg Leu Gly Glu Lys 
        435                 440                 445         


<210>  32
<211>  449
<212>  PRT
<213>  Cupriavidus basilensis

<400>  32

Met Glu Ala Val Ala Lys Lys Ser Ala Ala Thr Ile Ser Glu Ala Leu 
1               5                   10                  15      


Pro Ala Ala Ser Asn Arg Gln Val Phe Gly Ala Val Ala Ala Ser Cys 
            20                  25                  30          


Met Gly Trp Ala Leu Asp Leu Phe Asp Leu Phe Ile Leu Leu Phe Val 
        35                  40                  45              


Ala Pro Val Ile Gly Arg Leu Phe Phe Pro Ser Glu His Ala Met Leu 
    50                  55                  60                  


Ser Leu Ala Ala Val Tyr Ala Ser Phe Ala Val Thr Leu Leu Met Arg 
65                  70                  75                  80  


Pro Leu Gly Ser Ala Ile Phe Gly Ser Tyr Ala Asp Arg His Gly Arg 
                85                  90                  95      


Lys Gly Ala Met Val Val Ala Val Thr Gly Val Gly Leu Ser Thr Ala 
            100                 105                 110         


Ala Phe Gly Leu Leu Pro Thr Val Gly Gln Val Gly Leu Leu Ala Pro 
        115                 120                 125             


Ala Leu Phe Ile Leu Leu Arg Leu Val Gln Gly Ile Phe Val Gly Gly 
    130                 135                 140                 


Val Val Ala Ser Thr His Thr Ile Gly Thr Glu Ser Val Pro Pro Ser 
145                 150                 155                 160 


Trp Arg Gly Ala Val Ser Gly Leu Val Gly Gly Gly Gly Ala Gly Leu 
                165                 170                 175     


Gly Ala Leu Leu Ala Ser Ile Thr Tyr Met Ala Met Thr Ala Leu Phe 
            180                 185                 190         


Pro Gly Glu Ala Phe Asp Ala Trp Gly Trp Arg Cys Met Phe Phe Ser 
        195                 200                 205             


Gly Ile Ile Ser Ser Val Leu Gly Leu Phe Ile Phe Asn Ser Leu Glu 
    210                 215                 220                 


Glu Ser Pro Leu Trp Lys Gln Leu Gln Ala Ala Lys Gly His Ala Ala 
225                 230                 235                 240 


Pro Val Glu Asn Pro Leu Arg Val Ile Phe Ser Arg Gln Tyr Arg Gly 
                245                 250                 255     


Val Leu Phe Val Asn Ile Leu Leu Thr Val Gly Gly Gly Ser Ala Tyr 
            260                 265                 270         


Tyr Leu Thr Ser Gly Tyr Leu Pro Thr Phe Leu Lys Val Val Val Lys 
        275                 280                 285             


Ala Ser Ala Gly Glu Ser Ala Ala Ile Leu Met Ala Ser Ser Leu Gly 
    290                 295                 300                 


Val Ile Val Ala Ser Ile Leu Ala Gly His Leu Ser Thr Met Ile Gly 
305                 310                 315                 320 


Arg Lys Arg Ala Phe Leu Leu Ile Gly Ala Leu Asn Val Val Val Leu 
                325                 330                 335     


Pro Leu Leu Tyr Gln Trp Met Pro Ala Ala Pro Asp Thr Thr Thr Leu 
            340                 345                 350         


Gly Leu Tyr Ala Val Val Leu Ser Met Leu Gly Cys Ser Gly Phe Ala 
        355                 360                 365             


Pro Ile Leu Ile Phe Leu Asn Glu Arg Phe Pro Thr Ser Ile Arg Ala 
    370                 375                 380                 


Thr Gly Thr Gly Leu Ser Trp Asn Ile Gly Phe Ala Val Gly Gly Met 
385                 390                 395                 400 


Met Pro Thr Phe Ala Ser Leu Cys Ala Ser Thr Pro Ala Glu Leu Pro 
                405                 410                 415     


Met Val Leu Gly Ile Phe Leu Ala Val Val Thr Ile Ile Tyr Leu Val 
            420                 425                 430         


Gly Ala Phe Ile Val Pro Glu Thr Val Gly Arg Leu Gly Asp Asn Gly 
        435                 440                 445             


Ala 
    


<210>  33
<211>  449
<212>  PRT
<213>  Methylobacterium radiotolerans

<400>  33

Met Gln Thr Ala Ala Thr Phe Ala Ser Asp Pro Pro Ala Leu Ala Lys 
1               5                   10                  15      


Pro Thr Gly Arg Gln Thr Val Thr Ala Ala Met Ala Ser Leu Phe Gly 
            20                  25                  30          


Trp Gly Leu Asp Leu Phe Asp Leu Phe Ile Leu Leu Tyr Val Ala Pro 
        35                  40                  45              


Val Val Gly Thr Leu Phe Phe Pro Ala Asp Lys Pro Met Leu Ser Leu 
    50                  55                  60                  


Ala Gly Ala Tyr Ala Ser Phe Ala Val Thr Leu Leu Ile Arg Pro Leu 
65                  70                  75                  80  


Gly Ser Ala Leu Phe Gly Ser Tyr Ala Asp Arg Phe Gly Arg Arg Arg 
                85                  90                  95      


Ala Leu Met Val Ala Val Val Gly Val Gly Ile Ser Thr Ala Val Phe 
            100                 105                 110         


Gly Leu Leu Pro Thr Val Gly Gln Ile Gly Trp Leu Ala Thr Ala Val 
        115                 120                 125             


Phe Leu Phe Phe Arg Leu Val Gln Gly Ile Phe Val Gly Gly Val Val 
    130                 135                 140                 


Ala Ala Ser His Thr Ile Gly Thr Glu Ser Val Pro Glu Arg Trp Arg 
145                 150                 155                 160 


Gly Leu Met Ser Gly Ala Val Gly Gly Gly Gly Ser Ala Ile Gly Gly 
                165                 170                 175     


Leu Leu Ala Ser Leu Val Phe Tyr Val Val Ser Leu Met Ala Pro Gly 
            180                 185                 190         


Glu Ala Phe Ala Glu Trp Gly Trp Arg Leu Met Phe Phe Ser Gly Leu 
        195                 200                 205             


Leu Thr Ser Val Ile Gly Leu Ile Leu Phe Arg Asn Leu Glu Glu Ser 
    210                 215                 220                 


Pro Ile Phe Lys Glu Leu Gln Ala Arg Lys Ala Ala Leu Arg Ala Gly 
225                 230                 235                 240 


Ala Pro Ala Glu Ala Ser Pro Ile Arg Ser Leu Phe Ser Pro Ser Asn 
                245                 250                 255     


Arg Gly Ser Phe Ala Val Ala Thr Leu Ile Ser Phe Gly Gly Gly Ala 
            260                 265                 270         


Ala Tyr Tyr Leu Thr Ser Gly Tyr Leu Pro Thr Leu Leu Lys Leu Val 
        275                 280                 285             


Asn Gly Val Pro Asn Ala Thr Ala Ser Met Ile Leu Ile Gly Ala Asn 
    290                 295                 300                 


Val Ala Ala Ala Ile Gly Ala Cys Gly Met Gly Glu Leu Ser Gln His 
305                 310                 315                 320 


Ile Gly Arg Lys Arg Ser Phe Leu Leu Met Gly Val Ile Arg Leu Leu 
                325                 330                 335     


Ala Phe Pro Ala Leu Phe Leu Thr Met Ala Asn Thr Thr Ser Leu Val 
            340                 345                 350         


Gly Val Ala Ala Cys Ala Phe Leu Leu Ala Leu Ile Ala Asn Gly Ser 
        355                 360                 365             


Tyr Gly Pro Leu Leu Ile Phe Leu Asn Glu Lys Phe Pro Thr Ala Val 
    370                 375                 380                 


Arg Ala Thr Gly Thr Gly Leu Thr Trp Asn Ile Gly Phe Ala Leu Gly 
385                 390                 395                 400 


Gly Met Leu Pro Thr Leu Val Ser Leu Val Ala Asp Gly Pro Thr Gln 
                405                 410                 415     


Ile Pro Met Val Leu Ala Val Ile Thr Thr Gly Val Thr Leu Val Tyr 
            420                 425                 430         


Leu Val Gly Ala Phe Leu Thr Asp Glu Thr Gln Gly Asn Leu Asp Arg 
        435                 440                 445             


Ala 
    


<210>  34
<211>  443
<212>  PRT
<213>  Sulfolobus acidocaldarius

<400>  34

Met Lys Lys Glu Glu Lys Phe Thr Ser Asn His Phe Lys Trp Thr Leu 
1               5                   10                  15      


Ala Thr Phe Phe Thr Trp Thr Phe Asp Leu Tyr Asp Leu Phe Thr Ile 
            20                  25                  30          


Leu Leu Val Ala Pro Tyr Ile Ser Ser Leu Phe Phe Pro Ser Ser Ile 
        35                  40                  45              


Thr Phe Leu Ser Ile Ala Ala Thr Tyr Ala Gly Phe Ala Thr Ser Leu 
    50                  55                  60                  


Ile Met Arg Pro Val Gly Ala Thr Val Phe Gly Ser Arg Val Ser Asp 
65                  70                  75                  80  


Lys Val Gly Arg Lys Arg Ala Ile Phe Tyr Gly Leu Ile Gly Leu Val 
                85                  90                  95      


Ile Thr Ser Thr Leu Gln Gly Ala Leu Pro Thr Tyr Gln Val Val Gly 
            100                 105                 110         


Val Ile Ala Pro Ile Leu Leu Leu Ala Val Arg Leu Ile Gln Gly Val 
        115                 120                 125             


Phe Ile Gly Gly Ile Thr Ala Gly Ser His Val Ile Gly Pro Glu Ser 
    130                 135                 140                 


Val Pro Glu Arg Tyr Arg Gly Ile Val Gly Gly Leu Gly Phe Ser Ala 
145                 150                 155                 160 


Ala Gly Val Ala Tyr Leu Ile Ala Ala Gly Trp Phe Phe Leu Thr Thr 
                165                 170                 175     


Ile Leu Tyr Pro Gly Ser Ser Tyr Leu Val Trp Gly Trp Arg Val Met 
            180                 185                 190         


Phe Phe Gly Gly Leu Leu Ser Leu Ala Val Leu Gly Phe Val Asn Tyr 
        195                 200                 205             


Leu Val Pro Glu Ser Glu Val Trp Thr Lys Ile Lys Lys Arg Gly Ser 
    210                 215                 220                 


Val Val Lys Ser Pro Leu Lys Glu Ile Phe Ser Lys Tyr Arg Tyr Gln 
225                 230                 235                 240 


Leu Gly Val Ala Leu Leu Leu Ser Ile Gly Trp Gly Ala Ser Phe Tyr 
                245                 250                 255     


Val Thr Asp Gly Ile Leu Pro Thr Phe Leu Ser Ser Val Asn Lys Leu 
            260                 265                 270         


Ala Lys Thr Glu Ile Ala Ile Val Met Ile Ile Gly Ser Ile Gly Met 
        275                 280                 285             


Ser Ile Gly Pro Leu Ile Gly Gly Glu Ile Ser Gln Ile Ile Gly Arg 
    290                 295                 300                 


Lys Ile Thr Ser Leu Ile Gly Ala Ile Ile Val Leu Ala Val Val Gly 
305                 310                 315                 320 


Pro Leu Phe Leu Ser Leu Gly Ser Leu Lys Ser Gly Asp Leu Asn Gln 
                325                 330                 335     


Ile Ile Leu His Ser Phe Ala Ile Leu Phe Leu Val Asp Ile Gly Gly 
            340                 345                 350         


Gly Met Leu Met Thr Tyr Leu Asn Glu Ile Tyr Pro Ala Ser Val Arg 
        355                 360                 365             


Gly Thr Gly Val Gly Phe Thr Trp Asn Thr Gly Phe Ala Ile Gly Gly 
    370                 375                 380                 


Thr Ile Pro Thr Ile Ile Ser Leu Ala Val Ala Ser Ala Gly Leu Ser 
385                 390                 395                 400 


Ala Phe Pro Ser Ile Met Phe Tyr Thr Leu Ile Val Val Ser Val Ile 
                405                 410                 415     


Ile Leu Val Gly Thr Val Leu Thr Lys Glu Thr Lys Gly Thr Ile Ser 
            420                 425                 430         


Lys Glu Glu Tyr Glu Ile Gln Lys Glu Thr Leu 
        435                 440             


<210>  35
<211>  1240
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-NheI fragment for expression of alcohol 
       dehydrogenase  of Aneurinibacillus terranovensis

<400>  35
ggtaccgaat tccacatgac aaggggagac cgcatgacca ttagtccggc agttaaagcc     60

atcaactttg aattttcatt taacctgccg accctgatcg aatttggtta tggtaaaatg    120

gaaaaattcg gccagcagct gattagcatt ggtgttaaac gcatttttat ggtgaccgat    180

aaaggtgttg aaagcgcagg tctgctggca gcactgaccg attcactgca ggcagcagca    240

attcagtttg atatctatac cgatgtggaa agcgatccga gcctggaaac cattgatcgt    300

ggtgttgaag tttttcagca gaaaccgtat gattgcattg ttgcagttgg tggtggtagc    360

ccgattgata ccgcaaaagg tattcgtgtt gttgcagcaa atggtggtaa tattggtcat    420

tatgccggtg ttaatcagat tccggttgca ccgaccattc cgctgctggc aattccgacc    480

accagtggca ccggtagcga agttaccaat tttggtgttt atagcgattg gcagaacaac    540

gttaaagtta ccgttaccag ccagtatatg gcaccgacaa ttgcatgggt tgatccggca    600

ctgaccatga gcctgcctgc aaaaatgacc gcagcaagcg gtattgatgc actggcacat    660

ggtattgaaa ccttttttag cctgggtagc agtccggcaa gtgatgccct ggcaattgaa    720

gcaattcata ccgttaatcg ttatctgagc cgtgcagttc ataatggtag cgatatggaa    780

gcacgtattg gtatgagcca tggtagcctg ctggctggca tggcatttaa caatggtttt    840

ctgggtctgg cccatgccat tggtagcgca ctgagcggtc attgtcatgt tccgcatggt    900

gttgcaattg gtctgctgct gccgaaagtt gttgaattta atgcaaccgt tcgtccggat    960

aaagcagcaa aaattgcagg tctgatgggt atgaaaggtg aacatagcga agaactggcc   1020

ctgcaggcat caccggcagt tgcacgtctg gttgaagata ttggcctgcc gacacgtctg   1080

cgtgaagttg atgttaccga aaaaaaactg ttcgagatcg ccaaagatag ctttaaaagc   1140

ggcatgatga aattcaatcc gcgtcagccg agcgaaagcg aagttctgca gctgctgaaa   1200

gaaatctttt gaagaccgaa gcgaattcct cgagtctaga                         1240


<210>  36
<211>  1205
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-NheI fragment for expression of alcohol 
       dehydrogenase of  Brevibacillus thermoruber

<400>  36
ggtacctagg aaaggaagat taacccatga gccagaccgt gcagggcacc gacttcgcct     60

tcagcttcca cctgccgacc ctgatcgagt tcggctacgg ccgcgccagc cgcctgggcg    120

agcgcctgca gcacctgggc gtgaccaacg tgttcgtggt gaccgacaag ggcgtggagg    180

ccgccggcct gctgaacggc ctggtgggca gcctgcagag cgccggcatc gccttcgacc    240

tgtacaccga ggtggagccg gacccgggcc tggagaccat cgaccgcggc gccgccgtgt    300

tccgcgccaa gccgtacgac tgcctggtgg ccgtgggcgg cggcagcccg atcgacgccg    360

ccaagggcat gcgcgtggtg accagctgcg gcggcagcat cgccgactac gccggcgtga    420

accgcgtgcc gatggccccg gccgtgccgc tggtggccgt gccgaccacc agcggcaccg    480

gcagcgaggt gaccatgttc ggcgtgtaca gcgactggca caaccacgtg aaggtgaccg    540

tgaccagccc gcacatggcc ccgaccatcg ccctggtgga cccggccctg accgtgagcc    600

tgccggccaa gatgaccgcc gccagcggca tcgacgccct ggcccacggc atcgagacct    660

tcttcagcgt gcgcagccgc ccggccagcg acgccctggc catggaggcc atcgccgccg    720

tgaacgccca cctgcgccgc gccgtgcacg acggcagcga cgtggaggcc cgcatcggca    780

tgagccacgg cagcctgctg gccggcatgg ccttcaccaa cggcttcctg ggcctggccc    840

acgccatcgg cagcgccctg agcggccact gccacgtgcc gcacggcatc gccatcggcc    900

tgctgctgcc gcacgtggtg gccttcaacg ccccggcccg cccggacaag gccgcccagc    960

tggcccgcct gctgggcgtg gaggccaacc cgcgcgagga gcgcggcgag gagaccagcg   1020

ccgccgtggc ccgcatggtg gccgacatcg gcctgccgac ccgcctgcgc gacgtgggcg   1080

tgccggagga gaagctgccg gccatcgcca aggacgcctt caagagcggc atgatgacct   1140

gcaacccgcg ccagccgacc gagcaggagg tgcgcgagct gctgcgccgc gccttctgag   1200

ctagc                                                               1205


<210>  37
<211>  1228
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-NheI fragment for expression of alcohol 
       dehydrogenase of Brevibacillus panacihumi

<400>  37
ggtaccgaat tccacatgac aaggggagac cgcatgagcg caaatcagag cgttcagggt     60

attgaaagcc cgtttagctt tcatctgccg accaatgttc agtttggtgt tggtagcgca    120

agccgtctgg gtgaaatgct gctgagcatg ggtgttcgtc gtgtttttct ggttaccgat    180

cagggtgtgc gtcaggcagg tctgctggat gaagttattc atagcctgga agaaaaaggc    240

ctgcactttc agatttatgc agatgttgaa ccggatccga gcctggaaac cattcaggca    300

ggcgcagcaa tgtttcagca gcagagcttt gattgtatgg ttgcaattgg tggtggtagt    360

ccgattgata ccgcaaaagg tattcgtgtt ctggcagcaa atggtggcgg tattggtcag    420

tatgccggtg ttaatcgcgt tccggcagca agcgcaattc cgctgattgc aattccgacc    480

accagtggca ccggtagcga agttaccatt tttggtgttt atagcgattg ggagaaccac    540

gtgaaaatta ccgttaccag tccgcatatg gcaccgagca ccgcactgat tgatccggca    600

ctgaccctga gcctgcctgc aaaaatgacc gcagcaaccg gtattgatgc actggcacat    660

ggcattgaaa ccttttttag cctgcgtagc agtccggcaa gtgatgccct ggcaattcat    720

gcaatgaaaa tgattgcacc gcatctgcgt cgtgcagttc gtgatggtgc agatatggaa    780

gcacgtattg gtatgagcca gggtagcgtg ctggcaggta tggcatttaa caatggtttt    840

ctgggtctgg cccatgccat tggtagtgca ctgagcggtc attgtcatgt tccgcatggt    900

gttgcgattg gcctgctgct gccgcatgtg gttgcattta atacaccggt tcgtccggaa    960

aaagcagaac tgattgccga tgttctgggt agcgttcaga aagaaaccgg caccgcagcc   1020

gaactggttg gtcagctggt tcaggatatt ggtctgccgc agcgtctgca agaagttggc   1080

gttccggaag cgaaactggt tgatattgca aaagatagct ttaaaagcgg catgatgaaa   1140

tggaatccgc gtctgccgac agaacaagaa gttctggaac tgctgcagaa agccttttga   1200

agaccgaagc gaattcctcg agtctaga                                      1228


<210>  38
<211>  1184
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-NheI fragment for expression of alcohol 
       dehydrogenase of Bacillus sp. FJAT-14578

<400>  38
ggtacctagg aaaggaagat taacccatgt acccgagctt cgagttccac ctgccgacca     60

agatccactt cggctacaac accatcaagc agctggacca cctgccgttc gagatcaagc    120

gcgccttcat cgtgaccgac cagggcgtgc tgaacagcgg cctggtggag aacgtgacca    180

acatcctgaa ggaccaccag atcagctacg tgatctacag cgaggtggag ccggacccga    240

gcgtggagac cgtggacaag gccgcccaga tgttccagcg cgaggaggcc gacgccctga    300

tcgccatcgg cggcggcagc ccgatcgaca ccgccaaggg cgtgcgcgtg atcgccggca    360

acggcggcag catccgcgac tacgccggcg tgaacctgat caagcagaag agcaacatcc    420

cgctgatcgc catcccgacc accagcggca ccggcagcga ggtgaccatc ttcgccgtgt    480

tcagcgactg ggaggagaac cgcaaggtga ccgtgaccag cccgttcctg gccccggaca    540

tcagcatcgt ggacccgaag atgaccatga ccgccccgcc ggccatcacc gccgccagcg    600

gcttcgacgc cttcgcccac ggcgccgaga ccttcgtgag ccgcgccagc cagccggcca    660

gcgacgtgct ggccttcagc gccatgagca ccgtgagcaa gtacctgcgc cgcgccgtgt    720

acaacggcga ggacgtggag gcccgcatca agatggccga ggccagcctg ctggccggca    780

tggccttcaa ccagagctac ctgggcctga cccacgccat cggcagcgcc ctgagcggcc    840

acgcccacgt gagccacggc gtggccatcg gcctgctgct gccgggcgtg atccgctaca    900

acagcatcag ccgcatggac aagcacatcg agatggccgg cgccttccgc gagatcgacc    960

gcagcctgag cgactgggag atcatcgacc agctgatcga ggacgtgagc cgcctgcgcg   1020

acgacatcgg cctgccgcag cgcctgcagc aggtgggcgt gaaggaggac cagctgaaga   1080

tgatcgccgc cgacagcgtg aagagcggca tgtggaagtt caacccgcgc caggccagcg   1140

aggaggagat cctggagctg ctgaaggagc tgtactgagc tagc                    1184


<210>  39
<211>  1184
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic KpnI-NheI fragment for expression of alcohol 
       dehydrogenase of Bacillus sp. L1(2012)

<400>  39
ggtacctagg aaaggaagat taacccatgt acaccagctt caacttccac ctgccgaccc     60

gcatccagtt cggctacgag aaggtgaagg agctgaagaa cctgccgttc caggccaacc    120

gcgccttcat cgtgaccgac aagggcgtgg agaaggccgg cctgctgaac gacgtgatcg    180

acgccatcaa gcaggccaac atgacctaca agatctaccg cgacgtggag ccggacccga    240

gcgtggagac cgtggacaag gccgccaagg ccttcgccga ggccgagtgc gacctgctga    300

tcgccgtggg cggcggcagc ccgatcgaca ccgccaaggg cgtgcgcgtg gtggccagca    360

acggcggcag catccgcaac tacagcggcg tgaacctggt gaaggaggcc ccgagcgtgc    420

cgctggtggc catcccgacc accgccggca ccggcagcga ggtgaccatc ttcgccgtgt    480

tcagcgacga caaggagaac cgcaaggtga ccgtgaccag cagccacctg agcccggacg    540

tgagcatcat cgacccgaag ctgaccctga ccgccccgcc gagcatcacc gccgccgccg    600

gcttcgacgc cttcgcccac gccgccgagg ccttcgtgag ccgcatcagc cagccgccga    660

gcgacgccct ggccctgagc gccatgaaga ccgtgcacac ctacctgcgc cgcgccgtgt    720

acaacggcga cgacatcgag gcccgcatga agatggccga ggccagcctg ctggccggca    780

tggccttcaa ccagagctac ctgggcctgg cccacgccat cggcagcgcc atcagcgtgc    840

acgcccacgt gagccacggc gtggtgatcg gcctgctgct gccgaaggtg atcgagtaca    900

acctggtggc caagatcgac aagtacgccg aggccggcaa gtacatcgag cagagcagcc    960

acggcctgag caactacgag gccgccgccc tgttcagcga gaccgtgacc cagctgcgca   1020

acgacatcgg cctgccgaag cagctgcgcg aggtgaacgt gaaggaggcc cagctggagg   1080

ccatcagcaa ggacagcatc aagagcggca tgtggcagtt caacccgcgc cgcgccagcg   1140

agcaggacgt gtaccagatg ctgcgcgaga tgctgtgagc tagc                    1184


<210>  40
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  ribosome binding site containing spacer no. 2

<400>  40
gaattccaca tgacaagggg agaccgc                                         27


