
                                SEQUENCE LISTING

<110> The General Hospital Corporation

<120> THERAPEUTIC USE OF INTEGRIN-BINDING
  ANTIBODIES

<130> 29539-0166WO1

<140> PCT/US2016/020133   
<141> 2016-02-29  

<150> US 62/121,656       
<151> 2015-02-27  

<150> US 62/126,755       
<151> 2015-03-02  

<150> US 62/132,642       
<151> 2015-03-13  

<150> US 62/134,394       
<151> 2015-03-17  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 122
<212> PRT
<213> Mus musculus

<400> 1
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Thr Pro Ser Gly Phe Asn Ile Lys Asp Ile
            20                  25                  30          
Tyr Met Gln Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asp Pro Ala Asn Asp Lys Thr Lys Tyr Asp Pro Lys Phe
    50                  55                  60                  
Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr
65                  70                  75                  80  
Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Ser Glu Gly His Tyr Gly Tyr Asp Gly Tyr Ala Met Asp Tyr Trp
            100                 105                 110         
Gly Gln Gly Thr Thr Val Thr Val Ser Ser
        115                 120         


<210> 2
<211> 113
<212> PRT
<213> Mus musculus

<400> 2
Asp Ile Glu Met Thr Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly
 1               5                  10                  15      
Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Asn Leu Leu Tyr Ser
            20                  25                  30          
Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
        35                  40                  45              
Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
    50                  55                  60                  
Pro Asp Arg Phe Thr Gly Thr Gly Ser Gly Thr Asp Phe Thr Leu Thr
65                  70                  75                  80  
Ile Ser Ser Val Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln
                85                  90                  95      
Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
            100                 105                 110         
Lys
    


<210> 3
<211> 121
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized mAb107 VH domain

<400> 3
Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser
 1               5                  10                  15      
Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr
            20                  25                  30          
Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala
        35                  40                  45              
Arg Ile Asp Pro Ala Asn Asp Lys Thr Arg Tyr Ala Asp Ser Val Lys
    50                  55                  60                  
Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu
65                  70                  75                  80  
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser
                85                  90                  95      
Ser Glu Gly His Tyr Gly Tyr Asp Gly Tyr Ala Met Asp Tyr Trp Gly
            100                 105                 110         
Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120     


<210> 4
<211> 113
<212> PRT
<213> Artificial Sequence

<220> 
<223> Humanized mAb107 VL domain

<400> 4
Asp Ile Val Met Ser Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly
 1               5                  10                  15      
Glu Arg Val Thr Leu Asn Cys Lys Ser Ser Gln Asn Leu Leu Tyr Ser
            20                  25                  30          
Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
        35                  40                  45              
Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Ala Arg Glu Ser Gly Val
    50                  55                  60                  
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr
65                  70                  75                  80  
Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln
                85                  90                  95      
Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
            100                 105                 110         
Lys
    


<210> 5
<211> 250
<212> PRT
<213> Artificial Sequence

<220> 
<223> humanized single chain variable fragment of 107
      (scVF) with a linker linking VH and VL segments

<400> 5
Met Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr
            20                  25                  30          
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ala Arg Ile Asp Pro Ala Asn Asp Lys Thr Arg Tyr Ala Asp Ser Val
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ser Ser Glu Gly His Tyr Gly Tyr Asp Gly Tyr Ala Met Asp Tyr Trp
            100                 105                 110         
Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly
        115                 120                 125             
Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Ser Gln Ser
    130                 135                 140                 
Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Val Thr Leu Asn Cys
145                 150                 155                 160 
Lys Ser Ser Gln Asn Leu Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu
                165                 170                 175     
Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr
            180                 185                 190         
Trp Ala Ser Ala Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser
        195                 200                 205             
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu
    210                 215                 220                 
Asp Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr
225                 230                 235                 240 
Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
                245                 250 


<210> 6
<211> 6
<212> PRT
<213> Mus musculus

<400> 6
Gly Phe Asn Ile Lys Asp
 1               5      


<210> 7
<211> 6
<212> PRT
<213> Mus musculus

<400> 7
Asp Pro Ala Asn Asp Lys
 1               5      


<210> 8
<211> 8
<212> PRT
<213> Mus musculus

<400> 8
His Tyr Gly Tyr Asp Gly Tyr Ala
 1               5              


<210> 9
<211> 8
<212> PRT
<213> Mus musculus

<400> 9
Tyr Ser Ser Asn Gln Lys Asn Tyr
 1               5              


<210> 10
<211> 6
<212> PRT
<213> Mus musculus

<400> 10
Tyr Tyr Ser Tyr Pro Leu
 1               5      


<210> 11
<211> 224
<212> PRT
<213> Mus musculus

<400> 11
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala
 1               5                  10                  15      
Ser Val Lys Leu Ser Cys Thr Pro Ser Gly Phe Asn Ile Lys Asp Ile
            20                  25                  30          
Tyr Met Gln Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile
        35                  40                  45              
Gly Arg Ile Asp Pro Ala Asn Asp Lys Thr Lys Tyr Asp Pro Lys Phe
    50                  55                  60                  
Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr
65                  70                  75                  80  
Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95      
Ala Ser Glu Gly His Tyr Gly Tyr Asp Gly Tyr Ala Met Asp Tyr Trp
            100                 105                 110         
Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Lys Thr Thr Pro Pro
        115                 120                 125             
Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn Ser Met
    130                 135                 140                 
Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr
145                 150                 155                 160 
Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr Phe Pro
                165                 170                 175     
Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val Thr Val
            180                 185                 190         
Pro Ser Ser Pro Arg Pro Ser Glu Thr Val Thr Cys Asn Val Ala His
        195                 200                 205             
Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Asp Cys
    210                 215                 220                 


<210> 12
<211> 220
<212> PRT
<213> Mus musculus

<400> 12
Asp Ile Glu Met Thr Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly
 1               5                  10                  15      
Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Asn Leu Leu Tyr Ser
            20                  25                  30          
Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln
        35                  40                  45              
Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val
    50                  55                  60                  
Pro Asp Arg Phe Thr Gly Thr Gly Ser Gly Thr Asp Phe Thr Leu Thr
65                  70                  75                  80  
Ile Ser Ser Val Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln
                85                  90                  95      
Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu
            100                 105                 110         
Lys Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser
        115                 120                 125             
Glu Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn
    130                 135                 140                 
Phe Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu
145                 150                 155                 160 
Arg Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp
                165                 170                 175     
Ser Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr
            180                 185                 190         
Glu Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr
        195                 200                 205             
Ser Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys
    210                 215                 220 

