                         SEQUENCE LISTING

<110>  CureVac AG
 
<120>  Method for in vitro transcription using an immobilized 
       restriction enzyme

<130>  C 9809-WO2/RN

<150>  PCT/EP2015/059559
<151>  30.04.2015

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  277
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys 
        275         


<210>  2
<211>  300
<212>  PRT
<213>  Haemophilus influenzae

<400>  2

Met Lys Lys Ser Ala Leu Glu Lys Leu Leu Ser Leu Ile Glu Asn Leu 
1               5                   10                  15      


Thr Asn Gln Glu Phe Lys Gln Ala Thr Asn Ser Leu Ile Ser Phe Ile 
            20                  25                  30          


Tyr Lys Leu Asn Arg Asn Glu Val Ile Glu Leu Val Arg Ser Ile Gly 
        35                  40                  45              


Ile Leu Pro Glu Ala Ile Lys Pro Ser Ser Thr Gln Glu Lys Leu Phe 
    50                  55                  60                  


Ser Lys Ala Gly Asp Ile Val Leu Ala Lys Ala Phe Gln Leu Leu Asn 
65                  70                  75                  80  


Leu Asn Ser Lys Pro Leu Glu Gln Arg Gly Asn Ala Gly Asp Val Ile 
                85                  90                  95      


Ala Leu Ser Lys Glu Phe Asn Tyr Gly Leu Val Ala Asp Ala Lys Ser 
            100                 105                 110         


Phe Arg Leu Ser Arg Thr Ala Lys Asn Gln Lys Asp Phe Lys Val Lys 
        115                 120                 125             


Ala Leu Ser Glu Trp Arg Glu Asp Lys Asp Tyr Ala Val Leu Thr Ala 
    130                 135                 140                 


Pro Phe Phe Gln Tyr Pro Thr Thr Lys Ser Gln Ile Phe Lys Gln Ser 
145                 150                 155                 160 


Leu Asp Glu Asn Val Leu Leu Phe Ser Trp Glu His Leu Ala Ile Leu 
                165                 170                 175     


Leu Gln Leu Asp Leu Glu Glu Thr Asn Ile Phe Ser Phe Glu Gln Leu 
            180                 185                 190         


Trp Asn Phe Pro Lys Lys Gln Ser Lys Lys Thr Ser Val Ser Asp Ala 
        195                 200                 205             


Glu Asn Asn Phe Met Arg Asp Phe Asn Lys Tyr Phe Met Asp Leu Phe 
    210                 215                 220                 


Lys Ile Asp Lys Asp Thr Leu Asn Gln Leu Leu Gln Lys Glu Ile Asn 
225                 230                 235                 240 


Phe Ile Glu Glu Arg Ser Leu Ile Glu Lys Glu Tyr Trp Lys Lys Gln 
                245                 250                 255     


Ile Asn Ile Ile Lys Asn Phe Thr Arg Glu Glu Ala Ile Glu Ala Leu 
            260                 265                 270         


Leu Lys Asp Ile Asn Met Ser Ser Lys Ile Glu Thr Ile Asp Ser Phe 
        275                 280                 285             


Ile Lys Gly Ile Lys Ser Asn Asp Arg Leu Tyr Leu 
    290                 295                 300 


<210>  3
<211>  358
<212>  PRT
<213>  Bacillus circulans

<400>  3

Met Asn Phe Phe Ser Leu His Pro Asn Val Tyr Ala Thr Gly Arg Pro 
1               5                   10                  15      


Lys Gly Leu Ile Gly Met Leu Glu Asn Val Trp Val Ser Asn His Thr 
            20                  25                  30          


Pro Gly Glu Gly Thr Leu Tyr Leu Ile Ser Gly Phe Ser Asn Tyr Asn 
        35                  40                  45              


Gly Gly Val Arg Phe Tyr Glu Thr Phe Thr Glu His Ile Asn Gln Gly 
    50                  55                  60                  


Gly Arg Val Ile Ala Ile Leu Gly Gly Ser Thr Ser Gln Arg Leu Ser 
65                  70                  75                  80  


Ser Arg Gln Val Val Glu Glu Leu Leu Asn Arg Gly Val Glu Val His 
                85                  90                  95      


Ile Ile Asn Arg Lys Arg Ile Leu His Ala Lys Leu Tyr Gly Thr Ser 
            100                 105                 110         


Asn Asn Leu Gly Glu Ser Leu Val Val Ser Ser Gly Asn Phe Thr Gly 
        115                 120                 125             


Pro Gly Met Ser Gln Asn Ile Glu Ala Ser Leu Leu Leu Asp Asn Asn 
    130                 135                 140                 


Thr Thr Gln Ser Met Gly Phe Ser Trp Asn Asp Met Ile Ser Glu Met 
145                 150                 155                 160 


Leu Asn Gln Asn Trp His Ile His Asn Met Thr Asn Ala Thr Asp Ala 
                165                 170                 175     


Ser Pro Gly Trp Asn Leu Leu Tyr Asp Glu Arg Thr Thr Asn Leu Thr 
            180                 185                 190         


Leu Asp Glu Thr Glu Arg Val Thr Leu Ile Val Thr Leu Gly His Ala 
        195                 200                 205             


Asp Thr Ala Arg Ile Gln Ala Ala Pro Gly Thr Thr Ala Gly Gln Gly 
    210                 215                 220                 


Thr Gln Tyr Phe Trp Leu Ser Lys Asp Ser Tyr Asp Phe Phe Pro Pro 
225                 230                 235                 240 


Leu Thr Ile Arg Asn Arg Arg Gly Thr Lys Ala Thr Tyr Ser Ser Leu 
                245                 250                 255     


Ile Asn Met Asn Tyr Ile Asp Ile Asn Tyr Thr Asp Thr Gln Cys Arg 
            260                 265                 270         


Val Thr Phe Glu Ala Glu Asn Asn Phe Asp Phe Arg Leu Gly Thr Gly 
        275                 280                 285             


Lys Leu Arg Tyr Thr Gly Val Ala Lys Ser Asn Asp Ile Ala Ala Ile 
    290                 295                 300                 


Thr Arg Val Gly Asp Ser Asp Tyr Glu Leu Arg Ile Ile Lys Gln Gly 
305                 310                 315                 320 


Thr Pro Glu His Ser Gln Leu Asp Pro Tyr Ala Val Ser Phe Ile Gly 
                325                 330                 335     


Asn Arg Gly Lys Arg Phe Gly Tyr Ile Ser Asn Glu Glu Phe Gly Arg 
            340                 345                 350         


Ile Ile Gly Val Thr Phe 
        355             


<210>  4
<211>  277
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI K277C

<400>  4

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Cys 
        275         


<210>  5
<211>  277
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI K277C, C218A

<400>  5

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Cys 
        275         


<210>  6
<211>  277
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI K277C, C218S

<400>  6

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Cys 
        275         


<210>  7
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278(12xG)C

<400>  7

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
        275                 280                 285             


Gly Cys 
    290 


<210>  8
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278(12xG)C, C218A

<400>  8

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
        275                 280                 285             


Gly Cys 
    290 


<210>  9
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278(12xG)C, C218S

<400>  9

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
        275                 280                 285             


Gly Cys 
    290 


<210>  10
<211>  300
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HindIII L300C

<400>  10

Met Lys Lys Ser Ala Leu Glu Lys Leu Leu Ser Leu Ile Glu Asn Leu 
1               5                   10                  15      


Thr Asn Gln Glu Phe Lys Gln Ala Thr Asn Ser Leu Ile Ser Phe Ile 
            20                  25                  30          


Tyr Lys Leu Asn Arg Asn Glu Val Ile Glu Leu Val Arg Ser Ile Gly 
        35                  40                  45              


Ile Leu Pro Glu Ala Ile Lys Pro Ser Ser Thr Gln Glu Lys Leu Phe 
    50                  55                  60                  


Ser Lys Ala Gly Asp Ile Val Leu Ala Lys Ala Phe Gln Leu Leu Asn 
65                  70                  75                  80  


Leu Asn Ser Lys Pro Leu Glu Gln Arg Gly Asn Ala Gly Asp Val Ile 
                85                  90                  95      


Ala Leu Ser Lys Glu Phe Asn Tyr Gly Leu Val Ala Asp Ala Lys Ser 
            100                 105                 110         


Phe Arg Leu Ser Arg Thr Ala Lys Asn Gln Lys Asp Phe Lys Val Lys 
        115                 120                 125             


Ala Leu Ser Glu Trp Arg Glu Asp Lys Asp Tyr Ala Val Leu Thr Ala 
    130                 135                 140                 


Pro Phe Phe Gln Tyr Pro Thr Thr Lys Ser Gln Ile Phe Lys Gln Ser 
145                 150                 155                 160 


Leu Asp Glu Asn Val Leu Leu Phe Ser Trp Glu His Leu Ala Ile Leu 
                165                 170                 175     


Leu Gln Leu Asp Leu Glu Glu Thr Asn Ile Phe Ser Phe Glu Gln Leu 
            180                 185                 190         


Trp Asn Phe Pro Lys Lys Gln Ser Lys Lys Thr Ser Val Ser Asp Ala 
        195                 200                 205             


Glu Asn Asn Phe Met Arg Asp Phe Asn Lys Tyr Phe Met Asp Leu Phe 
    210                 215                 220                 


Lys Ile Asp Lys Asp Thr Leu Asn Gln Leu Leu Gln Lys Glu Ile Asn 
225                 230                 235                 240 


Phe Ile Glu Glu Arg Ser Leu Ile Glu Lys Glu Tyr Trp Lys Lys Gln 
                245                 250                 255     


Ile Asn Ile Ile Lys Asn Phe Thr Arg Glu Glu Ala Ile Glu Ala Leu 
            260                 265                 270         


Leu Lys Asp Ile Asn Met Ser Ser Lys Ile Glu Thr Ile Asp Ser Phe 
        275                 280                 285             


Ile Lys Gly Ile Lys Ser Asn Asp Arg Leu Tyr Cys 
    290                 295                 300 


<210>  11
<211>  301
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HindIII 301C

<400>  11

Met Lys Lys Ser Ala Leu Glu Lys Leu Leu Ser Leu Ile Glu Asn Leu 
1               5                   10                  15      


Thr Asn Gln Glu Phe Lys Gln Ala Thr Asn Ser Leu Ile Ser Phe Ile 
            20                  25                  30          


Tyr Lys Leu Asn Arg Asn Glu Val Ile Glu Leu Val Arg Ser Ile Gly 
        35                  40                  45              


Ile Leu Pro Glu Ala Ile Lys Pro Ser Ser Thr Gln Glu Lys Leu Phe 
    50                  55                  60                  


Ser Lys Ala Gly Asp Ile Val Leu Ala Lys Ala Phe Gln Leu Leu Asn 
65                  70                  75                  80  


Leu Asn Ser Lys Pro Leu Glu Gln Arg Gly Asn Ala Gly Asp Val Ile 
                85                  90                  95      


Ala Leu Ser Lys Glu Phe Asn Tyr Gly Leu Val Ala Asp Ala Lys Ser 
            100                 105                 110         


Phe Arg Leu Ser Arg Thr Ala Lys Asn Gln Lys Asp Phe Lys Val Lys 
        115                 120                 125             


Ala Leu Ser Glu Trp Arg Glu Asp Lys Asp Tyr Ala Val Leu Thr Ala 
    130                 135                 140                 


Pro Phe Phe Gln Tyr Pro Thr Thr Lys Ser Gln Ile Phe Lys Gln Ser 
145                 150                 155                 160 


Leu Asp Glu Asn Val Leu Leu Phe Ser Trp Glu His Leu Ala Ile Leu 
                165                 170                 175     


Leu Gln Leu Asp Leu Glu Glu Thr Asn Ile Phe Ser Phe Glu Gln Leu 
            180                 185                 190         


Trp Asn Phe Pro Lys Lys Gln Ser Lys Lys Thr Ser Val Ser Asp Ala 
        195                 200                 205             


Glu Asn Asn Phe Met Arg Asp Phe Asn Lys Tyr Phe Met Asp Leu Phe 
    210                 215                 220                 


Lys Ile Asp Lys Asp Thr Leu Asn Gln Leu Leu Gln Lys Glu Ile Asn 
225                 230                 235                 240 


Phe Ile Glu Glu Arg Ser Leu Ile Glu Lys Glu Tyr Trp Lys Lys Gln 
                245                 250                 255     


Ile Asn Ile Ile Lys Asn Phe Thr Arg Glu Glu Ala Ile Glu Ala Leu 
            260                 265                 270         


Leu Lys Asp Ile Asn Met Ser Ser Lys Ile Glu Thr Ile Asp Ser Phe 
        275                 280                 285             


Ile Lys Gly Ile Lys Ser Asn Asp Arg Leu Tyr Leu Cys 
    290                 295                 300     


<210>  12
<211>  359
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BciVI 359C

<400>  12

Met Asn Phe Phe Ser Leu His Pro Asn Val Tyr Ala Thr Gly Arg Pro 
1               5                   10                  15      


Lys Gly Leu Ile Gly Met Leu Glu Asn Val Trp Val Ser Asn His Thr 
            20                  25                  30          


Pro Gly Glu Gly Thr Leu Tyr Leu Ile Ser Gly Phe Ser Asn Tyr Asn 
        35                  40                  45              


Gly Gly Val Arg Phe Tyr Glu Thr Phe Thr Glu His Ile Asn Gln Gly 
    50                  55                  60                  


Gly Arg Val Ile Ala Ile Leu Gly Gly Ser Thr Ser Gln Arg Leu Ser 
65                  70                  75                  80  


Ser Arg Gln Val Val Glu Glu Leu Leu Asn Arg Gly Val Glu Val His 
                85                  90                  95      


Ile Ile Asn Arg Lys Arg Ile Leu His Ala Lys Leu Tyr Gly Thr Ser 
            100                 105                 110         


Asn Asn Leu Gly Glu Ser Leu Val Val Ser Ser Gly Asn Phe Thr Gly 
        115                 120                 125             


Pro Gly Met Ser Gln Asn Ile Glu Ala Ser Leu Leu Leu Asp Asn Asn 
    130                 135                 140                 


Thr Thr Gln Ser Met Gly Phe Ser Trp Asn Asp Met Ile Ser Glu Met 
145                 150                 155                 160 


Leu Asn Gln Asn Trp His Ile His Asn Met Thr Asn Ala Thr Asp Ala 
                165                 170                 175     


Ser Pro Gly Trp Asn Leu Leu Tyr Asp Glu Arg Thr Thr Asn Leu Thr 
            180                 185                 190         


Leu Asp Glu Thr Glu Arg Val Thr Leu Ile Val Thr Leu Gly His Ala 
        195                 200                 205             


Asp Thr Ala Arg Ile Gln Ala Ala Pro Gly Thr Thr Ala Gly Gln Gly 
    210                 215                 220                 


Thr Gln Tyr Phe Trp Leu Ser Lys Asp Ser Tyr Asp Phe Phe Pro Pro 
225                 230                 235                 240 


Leu Thr Ile Arg Asn Arg Arg Gly Thr Lys Ala Thr Tyr Ser Ser Leu 
                245                 250                 255     


Ile Asn Met Asn Tyr Ile Asp Ile Asn Tyr Thr Asp Thr Gln Cys Arg 
            260                 265                 270         


Val Thr Phe Glu Ala Glu Asn Asn Phe Asp Phe Arg Leu Gly Thr Gly 
        275                 280                 285             


Lys Leu Arg Tyr Thr Gly Val Ala Lys Ser Asn Asp Ile Ala Ala Ile 
    290                 295                 300                 


Thr Arg Val Gly Asp Ser Asp Tyr Glu Leu Arg Ile Ile Lys Gln Gly 
305                 310                 315                 320 


Thr Pro Glu His Ser Gln Leu Asp Pro Tyr Ala Val Ser Phe Ile Gly 
                325                 330                 335     


Asn Arg Gly Lys Arg Phe Gly Tyr Ile Ser Asn Glu Glu Phe Gly Arg 
            340                 345                 350         


Ile Ile Gly Val Thr Phe Cys 
        355                 


<210>  13
<211>  359
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BciVI 359C,C271A

<400>  13

Met Asn Phe Phe Ser Leu His Pro Asn Val Tyr Ala Thr Gly Arg Pro 
1               5                   10                  15      


Lys Gly Leu Ile Gly Met Leu Glu Asn Val Trp Val Ser Asn His Thr 
            20                  25                  30          


Pro Gly Glu Gly Thr Leu Tyr Leu Ile Ser Gly Phe Ser Asn Tyr Asn 
        35                  40                  45              


Gly Gly Val Arg Phe Tyr Glu Thr Phe Thr Glu His Ile Asn Gln Gly 
    50                  55                  60                  


Gly Arg Val Ile Ala Ile Leu Gly Gly Ser Thr Ser Gln Arg Leu Ser 
65                  70                  75                  80  


Ser Arg Gln Val Val Glu Glu Leu Leu Asn Arg Gly Val Glu Val His 
                85                  90                  95      


Ile Ile Asn Arg Lys Arg Ile Leu His Ala Lys Leu Tyr Gly Thr Ser 
            100                 105                 110         


Asn Asn Leu Gly Glu Ser Leu Val Val Ser Ser Gly Asn Phe Thr Gly 
        115                 120                 125             


Pro Gly Met Ser Gln Asn Ile Glu Ala Ser Leu Leu Leu Asp Asn Asn 
    130                 135                 140                 


Thr Thr Gln Ser Met Gly Phe Ser Trp Asn Asp Met Ile Ser Glu Met 
145                 150                 155                 160 


Leu Asn Gln Asn Trp His Ile His Asn Met Thr Asn Ala Thr Asp Ala 
                165                 170                 175     


Ser Pro Gly Trp Asn Leu Leu Tyr Asp Glu Arg Thr Thr Asn Leu Thr 
            180                 185                 190         


Leu Asp Glu Thr Glu Arg Val Thr Leu Ile Val Thr Leu Gly His Ala 
        195                 200                 205             


Asp Thr Ala Arg Ile Gln Ala Ala Pro Gly Thr Thr Ala Gly Gln Gly 
    210                 215                 220                 


Thr Gln Tyr Phe Trp Leu Ser Lys Asp Ser Tyr Asp Phe Phe Pro Pro 
225                 230                 235                 240 


Leu Thr Ile Arg Asn Arg Arg Gly Thr Lys Ala Thr Tyr Ser Ser Leu 
                245                 250                 255     


Ile Asn Met Asn Tyr Ile Asp Ile Asn Tyr Thr Asp Thr Gln Ala Arg 
            260                 265                 270         


Val Thr Phe Glu Ala Glu Asn Asn Phe Asp Phe Arg Leu Gly Thr Gly 
        275                 280                 285             


Lys Leu Arg Tyr Thr Gly Val Ala Lys Ser Asn Asp Ile Ala Ala Ile 
    290                 295                 300                 


Thr Arg Val Gly Asp Ser Asp Tyr Glu Leu Arg Ile Ile Lys Gln Gly 
305                 310                 315                 320 


Thr Pro Glu His Ser Gln Leu Asp Pro Tyr Ala Val Ser Phe Ile Gly 
                325                 330                 335     


Asn Arg Gly Lys Arg Phe Gly Tyr Ile Ser Asn Glu Glu Phe Gly Arg 
            340                 345                 350         


Ile Ile Gly Val Thr Phe Cys 
        355                 


<210>  14
<211>  359
<212>  PRT
<213>  Artificial sequence

<220>
<223>  BciVI 359C, C271S

<400>  14

Met Asn Phe Phe Ser Leu His Pro Asn Val Tyr Ala Thr Gly Arg Pro 
1               5                   10                  15      


Lys Gly Leu Ile Gly Met Leu Glu Asn Val Trp Val Ser Asn His Thr 
            20                  25                  30          


Pro Gly Glu Gly Thr Leu Tyr Leu Ile Ser Gly Phe Ser Asn Tyr Asn 
        35                  40                  45              


Gly Gly Val Arg Phe Tyr Glu Thr Phe Thr Glu His Ile Asn Gln Gly 
    50                  55                  60                  


Gly Arg Val Ile Ala Ile Leu Gly Gly Ser Thr Ser Gln Arg Leu Ser 
65                  70                  75                  80  


Ser Arg Gln Val Val Glu Glu Leu Leu Asn Arg Gly Val Glu Val His 
                85                  90                  95      


Ile Ile Asn Arg Lys Arg Ile Leu His Ala Lys Leu Tyr Gly Thr Ser 
            100                 105                 110         


Asn Asn Leu Gly Glu Ser Leu Val Val Ser Ser Gly Asn Phe Thr Gly 
        115                 120                 125             


Pro Gly Met Ser Gln Asn Ile Glu Ala Ser Leu Leu Leu Asp Asn Asn 
    130                 135                 140                 


Thr Thr Gln Ser Met Gly Phe Ser Trp Asn Asp Met Ile Ser Glu Met 
145                 150                 155                 160 


Leu Asn Gln Asn Trp His Ile His Asn Met Thr Asn Ala Thr Asp Ala 
                165                 170                 175     


Ser Pro Gly Trp Asn Leu Leu Tyr Asp Glu Arg Thr Thr Asn Leu Thr 
            180                 185                 190         


Leu Asp Glu Thr Glu Arg Val Thr Leu Ile Val Thr Leu Gly His Ala 
        195                 200                 205             


Asp Thr Ala Arg Ile Gln Ala Ala Pro Gly Thr Thr Ala Gly Gln Gly 
    210                 215                 220                 


Thr Gln Tyr Phe Trp Leu Ser Lys Asp Ser Tyr Asp Phe Phe Pro Pro 
225                 230                 235                 240 


Leu Thr Ile Arg Asn Arg Arg Gly Thr Lys Ala Thr Tyr Ser Ser Leu 
                245                 250                 255     


Ile Asn Met Asn Tyr Ile Asp Ile Asn Tyr Thr Asp Thr Gln Ser Arg 
            260                 265                 270         


Val Thr Phe Glu Ala Glu Asn Asn Phe Asp Phe Arg Leu Gly Thr Gly 
        275                 280                 285             


Lys Leu Arg Tyr Thr Gly Val Ala Lys Ser Asn Asp Ile Ala Ala Ile 
    290                 295                 300                 


Thr Arg Val Gly Asp Ser Asp Tyr Glu Leu Arg Ile Ile Lys Gln Gly 
305                 310                 315                 320 


Thr Pro Glu His Ser Gln Leu Asp Pro Tyr Ala Val Ser Phe Ile Gly 
                325                 330                 335     


Asn Arg Gly Lys Arg Phe Gly Tyr Ile Ser Asn Glu Glu Phe Gly Arg 
            340                 345                 350         


Ile Ile Gly Val Thr Phe Cys 
        355                 


<210>  15
<211>  278
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278C

<400>  15

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Cys 
        275             


<210>  16
<211>  278
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278C, C218A

<400>  16

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Cys 
        275             


<210>  17
<211>  278
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278C, C218S

<400>  17

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Cys 
        275             


<210>  18
<211>  3907
<212>  DNA
<213>  Artificial sequence

<220>
<223>  plasmid P1040

<400>  18
cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct       60

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca      120

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac      180

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt      240

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg      300

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc      360

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc      420

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc      480

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac      540

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt      600

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct      660

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc      720

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt      780

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg      840

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc      900

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa      960

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     1020

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     1080

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     1140

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     1200

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     1260

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     1320

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     1380

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     1440

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     1500

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     1560

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     1620

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     1680

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     1740

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     1800

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     1860

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     1920

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     1980

gtgccacctg acgtctaata cgactcacta tagggagaaa gcttaccatg gaggacgcca     2040

agaacatcaa gaagggcccg gcgcccttct acccgctgga ggacgggacc gccggcgagc     2100

agctccacaa ggccatgaag cggtacgccc tggtgccggg cacgatcgcc ttcaccgacg     2160

cccacatcga ggtcgacatc acctacgcgg agtacttcga gatgagcgtg cgcctggccg     2220

aggccatgaa gcggtacggc ctgaacacca accaccggat cgtggtgtgc tcggagaaca     2280

gcctgcagtt cttcatgccg gtgctgggcg ccctcttcat cggcgtggcc gtcgccccgg     2340

cgaacgacat ctacaacgag cgggagctgc tgaacagcat ggggatcagc cagccgaccg     2400

tggtgttcgt gagcaagaag ggcctgcaga agatcctgaa cgtgcagaag aagctgccca     2460

tcatccagaa gatcatcatc atggacagca agaccgacta ccagggcttc cagtcgatgt     2520

acacgttcgt gaccagccac ctcccgccgg gcttcaacga gtacgacttc gtcccggaga     2580

gcttcgaccg ggacaagacc atcgccctga tcatgaacag cagcggcagc accggcctgc     2640

cgaagggggt ggccctgccg caccggaccg cctgcgtgcg cttctcgcac gcccgggacc     2700

ccatcttcgg caaccagatc atcccggaca ccgccatcct gagcgtggtg ccgttccacc     2760

acggcttcgg catgttcacg accctgggct acctcatctg cggcttccgg gtggtcctga     2820

tgtaccggtt cgaggaggag ctgttcctgc ggagcctgca ggactacaag atccagagcg     2880

cgctgctcgt gccgaccctg ttcagcttct tcgccaagag caccctgatc gacaagtacg     2940

acctgtcgaa cctgcacgag atcgccagcg ggggcgcccc gctgagcaag gaggtgggcg     3000

aggccgtggc caagcggttc cacctcccgg gcatccgcca gggctacggc ctgaccgaga     3060

ccacgagcgc gatcctgatc acccccgagg gggacgacaa gccgggcgcc gtgggcaagg     3120

tggtcccgtt cttcgaggcc aaggtggtgg acctggacac cggcaagacc ctgggcgtga     3180

accagcgggg cgagctgtgc gtgcgggggc cgatgatcat gagcggctac gtgaacaacc     3240

cggaggccac caacgccctc atcgacaagg acggctggct gcacagcggc gacatcgcct     3300

actgggacga ggacgagcac ttcttcatcg tcgaccggct gaagtcgctg atcaagtaca     3360

agggctacca ggtggcgccg gccgagctgg agagcatcct gctccagcac cccaacatct     3420

tcgacgccgg cgtggccggg ctgccggacg acgacgccgg cgagctgccg gccgcggtgg     3480

tggtgctgga gcacggcaag accatgacgg agaaggagat cgtcgactac gtggccagcc     3540

aggtgaccac cgccaagaag ctgcggggcg gcgtggtgtt cgtggacgag gtcccgaagg     3600

gcctgaccgg gaagctcgac gcccggaaga tccgcgagat cctgatcaag gccaagaagg     3660

gcggcaagat cgccgtgtga ggactagtta taagactgac tagcccgatg ggcctcccaa     3720

cgggccctcc tcccctcctt gcaccgagat taataaaaaa aaaaaaaaaa aaaaaaaaaa     3780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaatg catccccccc cccccccccc     3840

cccccccccc ccccaaaggc tcttttcaga gccaccagaa ttcggatact ctagacatat     3900

gcttaag                                                               3907


<210>  19
<211>  1870
<212>  RNA
<213>  Artificial sequence

<220>
<223>  PpLuc (GC)GA- -A64-C30-histone stem-loop N5

<400>  19
gggagaaagc uuaccaugga ggacgccaag aacaucaaga agggcccggc gcccuucuac       60

ccgcuggagg acgggaccgc cggcgagcag cuccacaagg ccaugaagcg guacgcccug      120

gugccgggca cgaucgccuu caccgacgcc cacaucgagg ucgacaucac cuacgcggag      180

uacuucgaga ugagcgugcg ccuggccgag gccaugaagc gguacggccu gaacaccaac      240

caccggaucg uggugugcuc ggagaacagc cugcaguucu ucaugccggu gcugggcgcc      300

cucuucaucg gcguggccgu cgccccggcg aacgacaucu acaacgagcg ggagcugcug      360

aacagcaugg ggaucagcca gccgaccgug guguucguga gcaagaaggg ccugcagaag      420

auccugaacg ugcagaagaa gcugcccauc auccagaaga ucaucaucau ggacagcaag      480

accgacuacc agggcuucca gucgauguac acguucguga ccagccaccu cccgccgggc      540

uucaacgagu acgacuucgu cccggagagc uucgaccggg acaagaccau cgcccugauc      600

augaacagca gcggcagcac cggccugccg aagggggugg cccugccgca ccggaccgcc      660

ugcgugcgcu ucucgcacgc ccgggacccc aucuucggca accagaucau cccggacacc      720

gccauccuga gcguggugcc guuccaccac ggcuucggca uguucacgac ccugggcuac      780

cucaucugcg gcuuccgggu gguccugaug uaccgguucg aggaggagcu guuccugcgg      840

agccugcagg acuacaagau ccagagcgcg cugcucgugc cgacccuguu cagcuucuuc      900

gccaagagca cccugaucga caaguacgac cugucgaacc ugcacgagau cgccagcggg      960

ggcgccccgc ugagcaagga ggugggcgag gccguggcca agcgguucca ccucccgggc     1020

auccgccagg gcuacggccu gaccgagacc acgagcgcga uccugaucac ccccgagggg     1080

gacgacaagc cgggcgccgu gggcaaggug gucccguucu ucgaggccaa ggugguggac     1140

cuggacaccg gcaagacccu gggcgugaac cagcggggcg agcugugcgu gcgggggccg     1200

augaucauga gcggcuacgu gaacaacccg gaggccacca acgcccucau cgacaaggac     1260

ggcuggcugc acagcggcga caucgccuac ugggacgagg acgagcacuu cuucaucguc     1320

gaccggcuga agucgcugau caaguacaag ggcuaccagg uggcgccggc cgagcuggag     1380

agcauccugc uccagcaccc caacaucuuc gacgccggcg uggccgggcu gccggacgac     1440

gacgccggcg agcugccggc cgcgguggug gugcuggagc acggcaagac caugacggag     1500

aaggagaucg ucgacuacgu ggccagccag gugaccaccg ccaagaagcu gcggggcggc     1560

gugguguucg uggacgaggu cccgaagggc cugaccggga agcucgacgc ccggaagauc     1620

cgcgagaucc ugaucaaggc caagaagggc ggcaagaucg ccgugugagg acuaguuaua     1680

agacugacua gcccgauggg ccucccaacg ggcccuccuc cccuccuugc accgagauua     1740

auaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1800

aaaaaaugca uccccccccc cccccccccc cccccccccc ccaaaggcuc uuuucagagc     1860

caccagaauu                                                            1870


<210>  20
<211>  277
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI K277C, C218V

<400>  20

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Cys 
        275         


<210>  21
<211>  290
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278(12xG)C, C218V

<400>  21

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly 
        275                 280                 285             


Gly Cys 
    290 


<210>  22
<211>  278
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278C, C218V

<400>  22

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Cys 
        275             


<210>  23
<211>  284
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-HisTag-C

<400>  23

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys His His His His His His Cys 
        275                 280                 


<210>  24
<211>  284
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278HisTag-C, C218A

<400>  24

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys His His His His His His Cys 
        275                 280                 


<210>  25
<211>  284
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278HisTag-C, C218S

<400>  25

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys His His His His His His Cys 
        275                 280                 


<210>  26
<211>  284
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278HisTag-C, C218V

<400>  26

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys His His His His His His Cys 
        275                 280                 


<210>  27
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-FlexLinker-C

<400>  27

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys 
        275                 280                 285             


<210>  28
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278FlexLinker-C, C218A

<400>  28

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys 
        275                 280                 285             


<210>  29
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278FlexLinker-C, C218S

<400>  29

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys 
        275                 280                 285             


<210>  30
<211>  288
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278FlexLinker-C, C218V

<400>  30

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Cys 
        275                 280                 285             


<210>  31
<211>  294
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-FlexLinker-HisTag-C

<400>  31

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Cys Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His 
        275                 280                 285             


His His His His His Cys 
    290                 


<210>  32
<211>  294
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-FlexLinker-HisTag-C, C218A

<400>  32

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ala Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His 
        275                 280                 285             


His His His His His Cys 
    290                 


<210>  33
<211>  294
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-FlexLinker-HisTag-C, C218S

<400>  33

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Ser Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His 
        275                 280                 285             


His His His His His Cys 
    290                 


<210>  34
<211>  294
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EcoRI 278-FlexLinker-HisTag-C, C218V

<400>  34

Met Ser Asn Lys Lys Gln Ser Asn Arg Leu Thr Glu Gln His Lys Leu 
1               5                   10                  15      


Ser Gln Gly Val Ile Gly Ile Phe Gly Asp Tyr Ala Lys Ala His Asp 
            20                  25                  30          


Leu Ala Val Gly Glu Val Ser Lys Leu Val Lys Lys Ala Leu Ser Asn 
        35                  40                  45              


Glu Tyr Pro Gln Leu Ser Phe Arg Tyr Arg Asp Ser Ile Lys Lys Thr 
    50                  55                  60                  


Glu Ile Asn Glu Ala Leu Lys Lys Ile Asp Pro Asp Leu Gly Gly Thr 
65                  70                  75                  80  


Leu Phe Val Ser Asn Ser Ser Ile Lys Pro Asp Gly Gly Ile Val Glu 
                85                  90                  95      


Val Lys Asp Asp Tyr Gly Glu Trp Arg Val Val Leu Val Ala Glu Ala 
            100                 105                 110         


Lys His Gln Gly Lys Asp Ile Ile Asn Ile Arg Asn Gly Leu Leu Val 
        115                 120                 125             


Gly Lys Arg Gly Asp Gln Asp Leu Met Ala Ala Gly Asn Ala Ile Glu 
    130                 135                 140                 


Arg Ser His Lys Asn Ile Ser Glu Ile Ala Asn Phe Met Leu Ser Glu 
145                 150                 155                 160 


Ser His Phe Pro Tyr Val Leu Phe Leu Glu Gly Ser Asn Phe Leu Thr 
                165                 170                 175     


Glu Asn Ile Ser Ile Thr Arg Pro Asp Gly Arg Val Val Asn Leu Glu 
            180                 185                 190         


Tyr Asn Ser Gly Ile Leu Asn Arg Leu Asp Arg Leu Thr Ala Ala Asn 
        195                 200                 205             


Tyr Gly Met Pro Ile Asn Ser Asn Leu Val Ile Asn Lys Phe Val Asn 
    210                 215                 220                 


His Lys Asp Lys Ser Ile Met Leu Gln Ala Ala Ser Ile Tyr Thr Gln 
225                 230                 235                 240 


Gly Asp Gly Arg Glu Trp Asp Ser Lys Ile Met Phe Glu Ile Met Phe 
                245                 250                 255     


Asp Ile Ser Thr Thr Ser Leu Arg Val Leu Gly Arg Asp Leu Phe Glu 
            260                 265                 270         


Gln Leu Thr Ser Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser His 
        275                 280                 285             


His His His His His Cys 
    290                 


<210>  35
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  linker

<400>  35

Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Ser Leu Gln 
            20                  25      


<210>  36
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker_AEAAAKEAAAKA

<400>  36

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
1               5                   10          


<210>  37
<211>  46
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker

<400>  37

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


<210>  38
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)1

<400>  38

Glu Ala Ala Ala Lys 
1               5   


<210>  39
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)2

<400>  39

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10  


<210>  40
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker (EAAAK)3

<400>  40

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  41
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGG(GC)

<400>  41

Gly Gly Gly Gly 
1               


<210>  42
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGGGG(GC)

<400>  42

Gly Gly Gly Gly Gly 
1               5   


<210>  43
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gly-linker (G)6

<400>  43

Gly Gly Gly Gly Gly Gly 
1               5       


<210>  44
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Gly-linker (G)8

<400>  44

Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210>  45
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker GGGS

<400>  45

Gly Gly Gly Gly Ser 
1               5   


<210>  46
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-G4SG

<400>  46

Gly Gly Gly Gly Ser Gly 
1               5       


<210>  47
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-G4SG4(GC)

<400>  47

Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   


<210>  48
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-(G4S)2

<400>  48

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  49
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker (GGGGS)2

<400>  49

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  50
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-(GGGGS)3

<400>  50

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  51
<211>  20
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker (GGGGS)4

<400>  51

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  52
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGGSGGGGSGGGGSGG(GC)

<400>  52

Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


<210>  53
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GGSGGGGSGGGGSGG

<400>  53

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15  


<210>  54
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-GSG

<400>  54

Gly Ser Gly Ser 
1               


<210>  55
<211>  21
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker(GC)

<400>  55

Gly Ser Gly Gly Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10                  15      


Gly Gly Gly Gly Ser 
            20      


<210>  56
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Rigid-linker

<400>  56

Pro Ala Pro Ala Pro 
1               5   


<210>  57
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-PPPGSRHDKIH(GC)

<400>  57

Pro Pro Pro Gly Ser Arg His Asp Lys Ile His 
1               5                   10      


<210>  58
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-SGG(GC)

<400>  58

Ser Gly Gly Gly 
1               


<210>  59
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Linker-SGS

<400>  59

Gly Ser Gly Ser 
1               


<210>  60
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  19-A

<400>  60

Glu Trp Leu Lys Ala Phe Tyr Glu Lys Val Leu Glu Lys Leu Lys Glu 
1               5                   10                  15      


Leu 
    


<210>  61
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Avi-Tag

<400>  61

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  62
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Calmodulin Tag

<400>  62

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  63
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E-tag

<400>  63

Gly Ala Pro Val Pro Tyr Pro Asp Pro Leu Glu Pro Arg 
1               5                   10              


<210>  64
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ELK16-Tag

<400>  64

Leu Glu Leu Glu Leu Lys Leu Lys Leu Glu Leu Glu Leu Lys Leu Lys 
1               5                   10                  15      


<210>  65
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FLAG tag

<400>  65

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  66
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Poly-Glutamate-Tag

<400>  66

Glu Glu Glu Glu Glu Glu 
1               5       


<210>  67
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tag

<400>  67

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  68
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HIS tag

<400>  68

His His His His His His 
1               5       


<210>  69
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Isopeptag

<400>  69

Thr Asp Lys Asp Met Thr Ile Thr Phe Thr Asn Lys Lys Asp Ala Glu 
1               5                   10                  15      


<210>  70
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Myc Tag

<400>  70

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  71
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  S-tag

<400>  71

Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  


<210>  72
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SBP tag

<400>  72

Met Asp Glu Lys Thr Thr Gly Trp Arg Gly Gly His Val Val Glu Gly 
1               5                   10                  15      


Leu Ala Gly Glu Leu Glu Gln Leu Arg Ala Arg Leu Glu His His Pro 
            20                  25                  30          


Gln Gly Gln Arg Glu Pro 
        35              


<210>  73
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Spy Tag

<400>  73

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  74
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SofTag 1

<400>  74

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  75
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Sof Tag 2

<400>  75

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  76
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Strep-II-Tag

<400>  76

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  77
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7 Epitope tag

<400>  77

Met Ala Ser Met Thr Gly Gly Gln Gln Met Gly 
1               5                   10      


<210>  78
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  V5 Tag

<400>  78

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  79
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VSV Tag

<400>  79

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  80
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Xpress Tag

<400>  80

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


