                         SEQUENCE LISTING

<110>  H. LEE MOFFITT CANCER CENTER AND RESEARCH INSTITUTE, INC.
       THE WISTAR INSTITUTE
       Locke, Frederick
       Altieri, Dario
       Antonia, Scott
       Anasetti, Claudio
       Gabrilovich, Dmitry
 
<120>  Variant Survivin Vaccine for Treatment of Cancer

<130>  MOF.160XC1PCT

<150>  62/158,341
<151>  2015-05-07

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp 
1               5                   10                  15      


His Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala 
            20                  25                  30          


Cys Thr Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr 
        35                  40                  45              


Glu Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu 
    50                  55                  60                  


Glu Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His 
65                  70                  75                  80  


Ser Ser Gly Cys Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu 
                85                  90                  95      


Thr Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys 
            100                 105                 110         


Ile Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala 
        115                 120                 125             


Lys Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp 
    130                 135                 140         


<210>  2
<211>  142
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (34)..(34)
<223>  Xaa is any amino acid other than threonine

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa is any amino acid other than cysteine

<400>  2

Met Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp 
1               5                   10                  15      


His Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala 
            20                  25                  30          


Cys Xaa Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr 
        35                  40                  45              


Glu Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu 
    50                  55                  60                  


Glu Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His 
65                  70                  75                  80  


Ser Ser Gly Xaa Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu 
                85                  90                  95      


Thr Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys 
            100                 105                 110         


Ile Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala 
        115                 120                 125             


Lys Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp 
    130                 135                 140         


<210>  3
<211>  142
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Gly Ala Pro Thr Leu Pro Pro Ala Trp Gln Pro Phe Leu Lys Asp 
1               5                   10                  15      


His Arg Ile Ser Thr Phe Lys Asn Trp Pro Phe Leu Glu Gly Cys Ala 
            20                  25                  30          


Cys Ala Pro Glu Arg Met Ala Glu Ala Gly Phe Ile His Cys Pro Thr 
        35                  40                  45              


Glu Asn Glu Pro Asp Leu Ala Gln Cys Phe Phe Cys Phe Lys Glu Leu 
    50                  55                  60                  


Glu Gly Trp Glu Pro Asp Asp Asp Pro Ile Glu Glu His Lys Lys His 
65                  70                  75                  80  


Ser Ser Gly Ala Ala Phe Leu Ser Val Lys Lys Gln Phe Glu Glu Leu 
                85                  90                  95      


Thr Leu Gly Glu Phe Leu Lys Leu Asp Arg Glu Arg Ala Lys Asn Lys 
            100                 105                 110         


Ile Ala Lys Glu Thr Asn Asn Lys Lys Lys Glu Phe Glu Glu Thr Ala 
        115                 120                 125             


Lys Lys Val Arg Arg Ala Ile Glu Gln Leu Ala Ala Met Asp 
    130                 135                 140         


<210>  4
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for the C84A survivin mutant

<400>  4
cataaaaagc attcgtccgg tgccgctttc ctttctgtca agaag                       45


<210>  5
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for the C84A survivin mutant

<400>  5
cttcttgaca gaaaggaaag cggcaccgga cgaatgcttt ttatg                       45


<210>  6
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for the T34A survivin mutant

<400>  6
gagggctgcg cctgcgcccc ggagcggatg gcc                                    33


<210>  7
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  forward primer for the T34A survivin mutant

<400>  7
ggccatccgc tccggggcgc aggcgcagcc ctc                                    33


<210>  8
<211>  1190
<212>  DNA
<213>  Artificial

<220>
<223>  insert sequence

<400>  8
aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcatctg       60

tcgactgcta ccgccagatt tgaatcgcgg gacccgttgg cagaggtggc ggcggcggca      120

tgggtgcccc gacgttgccc cctgcctggc agccctttct caaggaccac cgcatctcta      180

cattcaagaa ctggcccttc ttggagggct gcgcctgcgc cccggagcgg atggccgagg      240

ctggcttcat ccactgcccc actgagaacg agccagactt ggcccagtgt ttcttctgct      300

tcaaggagct ggaaggctgg gagccagatg acgaccccat agaggaacat aaaaagcatt      360

cgtccggtgc cgctttcctt tctgtcaaga agcagtttga agaattaacc cttggtgaat      420

ttttgaaact ggacagagaa agagccaaga acaaaattgc aaaggaaacc aacaataaga      480

agaaagaatt tgaggaaact gcgaagaaag tgcgccgtgc catcgagcag ctggctgcca      540

tggattgagg cctctggccg gagctgcctg gtcccagagt ggctgcacca cttccagggt      600

ttattccctg gtgccaccag ccttcctgtg ggccccttag caatgtctta ggaaaggaga      660

tcaacatttt caaattagat gtttcaactg tgctcttgtt ttgtcttgaa agtggcacca      720

gaggtgcttc tgcctgtgca gcgggtgctg ctggtaacag tggctgcttc tctctctctc      780

tctctttttt gggggctcat ttttgctgtt ttgattcccg ggggatccta acatcgataa      840

aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt      900

ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa      960

tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga     1020

tatctgtggt aagcagttcc tgccccggct caggccaaga acagatggaa cagctgaata     1080

tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcaggcc aagaacagat     1140

ggtcccagat tgcggtccag ccctcagcag tttctaagat agatatccga                1190


