                         SEQUENCE LISTING

<110>  DUKE UNIVERSITY
       Poss, Kenneth D.
       Mokalled, Mayssa H.
 
<120>  Compositions and Methods for Modulating Spinal Cord Structure and
        Function

<130>  16-665-WO

<150>  US 62/159,413
<151>  2015-05-11

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ctgfa forward primer

<400>  1
tgtgattgct ctgctgttcc                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ctgfa reverse primer

<400>  2
ggtgaggcga ttagcttctg                                                   20


<210>  3
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI forward primer

<400>  3
atcgattttg gctactttca gctaagactg g                                      31


<210>  4
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI reverse primer

<400>  4
atcgattctt taaagtttgt agcaaaaaga aa                                     32


<210>  5
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcoRV forward primer

<400>  5
ccgatatcgc caccatgttt tctggaatga ctcaaagtgt gattgc                      46


<210>  6
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI reverse primer

<400>  6
ccgatatcgc caccatgttt tctggaatga ctcaaagtgt gattgc                      46


<210>  7
<211>  57
<212>  DNA
<213>  Danio rerio

<400>  7
ggagagtctt tccagagcag ttgtaaatac cagtcgacgt gtctggacgg tgctgta          57


<210>  8
<211>  19
<212>  PRT
<213>  Danio rerio

<400>  8

Gly Glu Ser Phe Glx Ser Ser Cys Lys Tyr Glx Cys Thr Cys Leu Asp 
1               5                   10                  15      


Gly Ala Val 
            


<210>  9
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ctgfa bns50 oligonucleotide

<400>  9
ggagagtctt tccagagcag taccagtgca cgtgtctgga cggtgctgta g                51


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ctgfa bns50 peptide

<400>  10

Gly Glu Ser Phe Gln Ser Ser Thr Ser Ala Arg Val Trp Thr Val Leu 
1               5                   10                  15      


<210>  11
<211>  349
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Thr Ala Ala Ser Met Gly Pro Val Arg Val Ala Phe Val Val Leu 
1               5                   10                  15      


Leu Ala Leu Cys Ser Arg Pro Ala Val Gly Gln Asn Cys Ser Gly Pro 
            20                  25                  30          


Cys Arg Cys Pro Asp Glu Pro Ala Pro Arg Cys Pro Ala Gly Val Ser 
        35                  40                  45              


Leu Val Leu Asp Gly Cys Gly Cys Cys Arg Val Cys Ala Lys Gln Leu 
    50                  55                  60                  


Gly Glu Leu Cys Thr Glu Arg Asp Pro Cys Asp Pro His Lys Gly Leu 
65                  70                  75                  80  


Phe Cys Asp Phe Gly Ser Pro Ala Asn Arg Lys Ile Gly Val Cys Thr 
                85                  90                  95      


Ala Lys Asp Gly Ala Pro Cys Ile Phe Gly Gly Thr Val Tyr Arg Ser 
            100                 105                 110         


Gly Glu Ser Phe Gln Ser Ser Cys Lys Tyr Gln Cys Thr Cys Leu Asp 
        115                 120                 125             


Gly Ala Val Gly Cys Met Pro Leu Cys Ser Met Asp Val Arg Leu Pro 
    130                 135                 140                 


Ser Pro Asp Cys Pro Phe Pro Arg Arg Val Lys Leu Pro Gly Lys Cys 
145                 150                 155                 160 


Cys Glu Glu Trp Val Cys Asp Glu Pro Lys Asp Gln Thr Val Val Gly 
                165                 170                 175     


Pro Ala Leu Ala Ala Tyr Arg Leu Glu Asp Thr Phe Gly Pro Asp Pro 
            180                 185                 190         


Thr Met Ile Arg Ala Asn Cys Leu Val Gln Thr Thr Glu Trp Ser Ala 
        195                 200                 205             


Cys Ser Lys Thr Cys Gly Met Gly Ile Ser Thr Arg Val Thr Asn Asp 
    210                 215                 220                 


Asn Ala Ser Cys Arg Leu Glu Lys Gln Ser Arg Leu Cys Met Val Arg 
225                 230                 235                 240 


Pro Cys Glu Ala Asp Leu Glu Glu Asn Ile Lys Lys Gly Lys Lys Cys 
                245                 250                 255     


Ile Arg Thr Pro Lys Ile Ser Lys Pro Ile Lys Phe Glu Leu Ser Gly 
            260                 265                 270         


Cys Thr Ser Met Lys Thr Tyr Arg Ala Lys Phe Cys Gly Val Cys Thr 
        275                 280                 285             


Asp Gly Arg Cys Cys Thr Pro His Arg Thr Thr Thr Leu Pro Val Glu 
    290                 295                 300                 


Phe Lys Cys Pro Asp Gly Glu Val Met Lys Lys Asn Met Met Phe Ile 
305                 310                 315                 320 


Lys Thr Cys Ala Cys His Tyr Asn Cys Pro Gly Asp Asn Asp Ile Phe 
                325                 330                 335     


Glu Ser Leu Tyr Tyr Arg Lys Met Tyr Gly Asp Met Ala 
            340                 345                 


<210>  12
<211>  348
<212>  PRT
<213>  Mus musculus

<400>  12

Met Leu Ala Ser Val Ala Gly Pro Ile Ser Leu Ala Leu Val Leu Leu 
1               5                   10                  15      


Ala Leu Cys Thr Arg Pro Ala Met Gly Gln Asp Cys Ser Ala Gln Cys 
            20                  25                  30          


Gln Cys Ala Ala Glu Ala Ala Pro His Cys Pro Ala Gly Val Ser Leu 
        35                  40                  45              


Val Leu Asp Gly Cys Gly Cys Cys Arg Val Cys Ala Lys Gln Leu Gly 
    50                  55                  60                  


Glu Leu Cys Thr Glu Arg Asp Pro Cys Asp Pro His Lys Gly Leu Phe 
65                  70                  75                  80  


Cys Asp Phe Gly Ser Pro Ala Asn Arg Lys Ile Gly Val Cys Thr Ala 
                85                  90                  95      


Lys Asp Gly Ala Pro Cys Val Phe Gly Gly Ser Val Tyr Arg Ser Gly 
            100                 105                 110         


Glu Ser Phe Gln Ser Ser Cys Lys Tyr Gln Cys Thr Cys Leu Asp Gly 
        115                 120                 125             


Ala Val Gly Cys Val Pro Leu Cys Ser Met Asp Val Arg Leu Pro Ser 
    130                 135                 140                 


Pro Asp Cys Pro Phe Pro Arg Arg Val Lys Leu Pro Gly Lys Cys Cys 
145                 150                 155                 160 


Glu Glu Trp Val Cys Asp Glu Pro Lys Asp Arg Thr Ala Val Gly Pro 
                165                 170                 175     


Ala Leu Ala Ala Tyr Arg Leu Glu Asp Thr Phe Gly Pro Asp Pro Thr 
            180                 185                 190         


Met Met Arg Ala Asn Cys Leu Val Gln Thr Thr Glu Trp Ser Ala Cys 
        195                 200                 205             


Ser Lys Thr Cys Gly Met Gly Ile Ser Thr Arg Val Thr Asn Asp Asn 
    210                 215                 220                 


Thr Phe Cys Arg Leu Glu Lys Gln Ser Arg Leu Cys Met Val Arg Pro 
225                 230                 235                 240 


Cys Glu Ala Asp Leu Glu Glu Asn Ile Lys Lys Gly Lys Lys Cys Ile 
                245                 250                 255     


Arg Thr Pro Lys Ile Ala Lys Pro Val Lys Phe Glu Leu Ser Gly Cys 
            260                 265                 270         


Thr Ser Val Lys Thr Tyr Arg Ala Lys Phe Cys Gly Val Cys Thr Asp 
        275                 280                 285             


Gly Arg Cys Cys Thr Pro His Arg Thr Thr Thr Leu Pro Val Glu Phe 
    290                 295                 300                 


Lys Cys Pro Asp Gly Glu Ile Met Lys Lys Asn Met Met Phe Ile Lys 
305                 310                 315                 320 


Thr Cys Ala Cys His Tyr Asn Cys Pro Gly Asp Asn Asp Ile Phe Glu 
                325                 330                 335     


Ser Leu Tyr Tyr Arg Lys Met Tyr Gly Asp Met Ala 
            340                 345             


<210>  13
<211>  345
<212>  PRT
<213>  Danio rerio

<400>  13

Met Phe Ser Gly Met Thr Gln Ser Val Ile Ala Leu Leu Phe Leu Thr 
1               5                   10                  15      


Phe Leu Arg Trp Ala Val Ala Gln Glu Cys Ser Gly Gln Cys His Cys 
            20                  25                  30          


Pro Glu Val Pro Pro Gln Cys Ser Pro Gly Val Ser Leu Val Leu Asp 
        35                  40                  45              


Thr Cys Gly Cys Cys Arg Val Cys Ala Lys Gln Leu Gly Glu Leu Cys 
    50                  55                  60                  


Thr Glu Arg Asp Val Cys Asp Pro His Lys Gly Leu Tyr Cys Asp Tyr 
65                  70                  75                  80  


Gly Ser Pro Ser Asn Arg Arg Ile Gly Val Cys Thr Ala Arg Asp Gly 
                85                  90                  95      


Ala Thr Cys Val Phe Gly Gly Met Val Tyr Arg Ser Gly Glu Ser Phe 
            100                 105                 110         


Gln Ser Ser Cys Lys Tyr Gln Cys Thr Cys Leu Asp Gly Ala Val Gly 
        115                 120                 125             


Cys Val Pro Leu Cys Gly Met Asp Ile Arg Leu Pro Ser Pro Asp Cys 
    130                 135                 140                 


Pro Met Pro Arg Arg Val Lys Val Pro Gly Lys Cys Cys Glu Glu Trp 
145                 150                 155                 160 


Val Cys Asp Ser Pro Arg Gln Asn Thr Phe Val Gly Ser Ala Leu Ala 
                165                 170                 175     


Ala Tyr Arg Glu Glu Glu Thr Tyr Gly Pro Asp Pro Ser Met Met Arg 
            180                 185                 190         


Glu Asn Cys Leu Val Gln Thr Thr Glu Trp Ser Ala Cys Ser Lys Thr 
        195                 200                 205             


Cys Gly Leu Gly Ile Ser Thr Arg Val Thr Asn Asp Asn Arg Glu Cys 
    210                 215                 220                 


Arg Leu Glu Lys Gln Ser Arg Leu Cys Met Val Arg Pro Cys Glu Ser 
225                 230                 235                 240 


His Leu Glu Glu Lys Ile Arg Lys Gly Lys Lys Cys Ile Arg Thr Pro 
                245                 250                 255     


Arg Val Ser Lys Pro Met Lys Phe Glu Ile Ser Gly Cys Thr Thr Thr 
            260                 265                 270         


Lys Ser Tyr Arg Pro Lys Phe Cys Gly Val Cys Thr Asp Gly Arg Cys 
        275                 280                 285             


Cys Thr Pro His Arg Thr Ala Thr Leu Pro Met Glu Phe Lys Cys Pro 
    290                 295                 300                 


Asp Gly Gln Val Met Lys Lys Gln Met Met Phe Ile Lys Thr Cys Ala 
305                 310                 315                 320 


Cys His Tyr Asn Cys Pro Gly Glu Asn Asp Ile Phe Glu Ser Met Tyr 
                325                 330                 335     


Tyr Lys Lys Met Val Gly Asp Met Ala 
            340                 345 


<210>  14
<211>  347
<212>  PRT
<213>  Danio rerio

<400>  14

Met Thr Gly Thr Ala Phe Thr Arg Ala Cys Ile Ala Leu Ser Leu Leu 
1               5                   10                  15      


Ile His Thr Ala Ile Cys Gln Asp Cys Ser Gln Pro Cys Asp Cys Pro 
            20                  25                  30          


Asp Glu Ser Pro Leu Cys Pro Val Gly Thr Ser Leu Val Leu Asp Gly 
        35                  40                  45              


Cys Ser Cys Cys Lys Val Cys Ala Arg Gln Ala Gly Glu Pro Cys Ser 
    50                  55                  60                  


Phe Leu Glu Pro Cys Asp His His Lys Gly Leu Tyr Cys Asp Tyr Ser 
65                  70                  75                  80  


Val Leu Ser Asp Thr Glu Thr Gly Ile Cys Met Ala Gln Glu Gly Gln 
                85                  90                  95      


Thr Cys Asp Leu Gly Gly Val Ile Tyr Arg Ser Gly Glu Thr Phe Gln 
            100                 105                 110         


Pro Ser Cys Lys His Gln Cys Val Ser Met Asn Gly Glu Ile Gly Cys 
        115                 120                 125             


Val Pro Thr Cys Ala Thr Ile Ile Arg Leu Pro Ser Pro Asp Cys Pro 
    130                 135                 140                 


Tyr Pro Arg Arg Val Gln Ile Pro Gly Lys Cys Cys Glu Glu Trp Val 
145                 150                 155                 160 


Cys Asp Gln Ile Pro Gln Glu Asp Thr Phe Gln Ser Ala Val Ala Ala 
                165                 170                 175     


Tyr Arg Glu Ile Ser Ala His Gly Leu Gln Pro Glu Ser Ala Arg Asp 
            180                 185                 190         


Asn Cys Ile Val Gln Thr Thr Glu Trp Ser Glu Cys Ser Ala Thr Cys 
        195                 200                 205             


Gly Met Gly Val Ser Ser Arg Val Thr Asn Asp Asn Glu Gln Cys Gln 
    210                 215                 220                 


Leu Glu Arg Gln Thr Arg Met Cys Met Ile Arg Pro Cys His Ala Asp 
225                 230                 235                 240 


Leu Glu Lys Asp Ile Lys Arg Gly Lys Lys Cys Val Arg Thr Pro Lys 
                245                 250                 255     


Ser Gln Ser Gly Met Arg Phe Glu Leu Ser Gly Cys Gln Ser Ile Arg 
            260                 265                 270         


Leu Tyr Lys Pro Lys Phe Cys Gly Val Cys Thr Asp Asp Arg Cys Cys 
        275                 280                 285             


Thr Pro Gln Ser Thr Ile Thr Ala Glu Val Glu Phe Arg Cys Pro Glu 
    290                 295                 300                 


Gly Asp Ser Phe Arg Lys Lys Met Met Phe Ile Lys Thr Cys Ser Cys 
305                 310                 315                 320 


His His Asn Cys Pro Arg Glu Asn Asp Ile Phe Leu Ala Ser Asn Ser 
                325                 330                 335     


Arg Arg Met Ile Gly Asp Tyr Glu Asn Asp Met 
            340                 345         


<210>  15
<211>  99
<212>  PRT
<213>  Homo sapiens

<400>  15

Lys Lys Gly Lys Lys Cys Ile Arg Thr Pro Lys Ile Ser Lys Pro Ile 
1               5                   10                  15      


Lys Phe Glu Leu Ser Gly Cys Thr Ser Met Lys Thr Tyr Arg Ala Lys 
            20                  25                  30          


Phe Cys Gly Val Cys Thr Asp Gly Arg Cys Cys Thr Pro His Arg Thr 
        35                  40                  45              


Thr Thr Leu Pro Val Glu Phe Lys Cys Pro Asp Gly Glu Val Met Lys 
    50                  55                  60                  


Lys Asn Met Met Phe Ile Lys Thr Cys Ala Cys His Tyr Asn Cys Pro 
65                  70                  75                  80  


Gly Asp Asn Asp Ile Phe Glu Ser Leu Tyr Tyr Arg Lys Met Tyr Gly 
                85                  90                  95      


Asp Met Ala 
            


