                         SEQUENCE LISTING

<110>  UCL Business PLC
 
<120>  Cell

<130>  P106294PCT

<150>  GB 1509413.9
<151>  2015-06-01

<160>  63    

<170>  PatentIn version 3.5

<210>  1
<211>  619
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Gln Arg Arg Ile Asp Thr Leu Asn 
        275                 280                 285             


Ser Asp Gly Tyr Thr Pro Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys 
    290                 295                 300                 


Pro Arg Pro Met Pro Met Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser 
305                 310                 315                 320 


Asp Pro Glu Glu Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn 
                325                 330                 335     


Leu Leu Ile Ala Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val 
            340                 345                 350         


Arg Gln Gly Val Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile 
        355                 360                 365             


Lys Val Leu Lys Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met 
    370                 375                 380                 


Arg Glu Ala Gln Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg 
385                 390                 395                 400 


Leu Ile Gly Val Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met 
                405                 410                 415     


Ala Gly Gly Gly Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu 
            420                 425                 430         


Ile Pro Val Ser Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly 
        435                 440                 445             


Met Lys Tyr Leu Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala 
    450                 455                 460                 


Arg Asn Val Leu Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe 
465                 470                 475                 480 


Gly Leu Ser Lys Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg 
                485                 490                 495     


Ser Ala Gly Lys Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn 
            500                 505                 510         


Phe Arg Lys Phe Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr 
        515                 520                 525             


Met Trp Glu Ala Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys 
    530                 535                 540                 


Gly Pro Glu Val Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys 
545                 550                 555                 560 


Pro Pro Glu Cys Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp 
                565                 570                 575     


Ile Tyr Lys Trp Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg 
            580                 585                 590         


Met Arg Ala Cys Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro 
        595                 600                 605             


Gly Ser Thr Gln Lys Ala Glu Ala Ala Cys Ala 
    610                 615                 


<210>  2
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 complete SH2 domain

<400>  2

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro 
        275                 280 


<210>  3
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 1

<400>  3

Phe Phe Tyr Gly Ser Ile Ser Arg Ala Glu Ala Glu Glu His Leu Lys 
1               5                   10                  15      


Leu Ala Gly Met Ala Asp Gly Leu Phe Leu Leu Arg Gln Cys Leu Arg 
            20                  25                  30          


Ser Leu Gly Gly Tyr Val Leu Ser Leu Val His Asp Val Arg Phe His 
        35                  40                  45              


His Phe Pro Ile Glu Arg Gln Leu Asn Gly Thr Tyr Ala Ile Ala Gly 
    50                  55                  60                  


Gly Lys Ala His Cys Gly Pro Ala Glu Leu Cys Glu Phe Tyr Ser Arg 
65                  70                  75                  80  


Asp Pro Asp Gly Leu Pro Cys Asn Leu Arg Lys Pro Cys 
                85                  90              


<210>  4
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 2

<400>  4

Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys Leu Tyr 
1               5                   10                  15      


Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg Lys Glu 
            20                  25                  30          


Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val Tyr His 
        35                  40                  45              


Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro Glu Gly 
    50                  55                  60                  


Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys Leu Lys 
65                  70                  75                  80  


Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys 
                85                  90          


<210>  5
<211>  595
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr Ala Thr Arg Val Asn Ala Ala Asp Ile Glu 
    210                 215                 220                 


Asn Arg Val Leu Glu Leu Asn Lys Lys Gln Glu Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Lys Ala Gly Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val 
                245                 250                 255     


Lys Asn Leu His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly 
            260                 265                 270         


Lys Asn Arg Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile 
        275                 280                 285             


Leu Gln Gly Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala 
    290                 295                 300                 


Asn Tyr Ile Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr 
305                 310                 315                 320 


Tyr Ile Ala Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp 
                325                 330                 335     


Gln Met Ala Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg 
            340                 345                 350         


Glu Val Glu Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val 
        355                 360                 365             


Gly Met Gln Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu 
    370                 375                 380                 


His Asp Thr Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu 
385                 390                 395                 400 


Asp Asn Gly Asp Leu Ile Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser 
                405                 410                 415     


Trp Pro Asp His Gly Val Pro Ser Glu Pro Gly Gly Val Leu Ser Phe 
            420                 425                 430         


Leu Asp Gln Ile Asn Gln Arg Gln Glu Ser Leu Pro His Ala Gly Pro 
        435                 440                 445             


Ile Ile Val His Cys Ser Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile 
    450                 455                 460                 


Val Ile Asp Met Leu Met Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys 
465                 470                 475                 480 


Asp Ile Asp Ile Gln Lys Thr Ile Gln Met Val Arg Ala Gln Arg Ser 
                485                 490                 495     


Gly Met Val Gln Thr Glu Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile 
            500                 505                 510         


Ala Gln Phe Ile Glu Thr Thr Lys Lys Lys Leu Glu Val Leu Gln Ser 
        515                 520                 525             


Gln Lys Gly Gln Glu Ser Glu Tyr Gly Asn Ile Thr Tyr Pro Pro Ala 
    530                 535                 540                 


Met Lys Asn Ala His Ala Lys Ala Ser Arg Thr Ser Ser Lys His Lys 
545                 550                 555                 560 


Glu Asp Val Tyr Glu Asn Leu His Thr Lys Asn Lys Arg Glu Glu Lys 
                565                 570                 575     


Val Lys Lys Gln Arg Ser Ala Asp Lys Glu Lys Ser Lys Gly Ser Leu 
            580                 585                 590         


Lys Arg Lys 
        595 


<210>  6
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6 SH2 complete domain

<400>  6

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr 
    210                 


<210>  7
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6 SH2 1

<400>  7

Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr Leu Leu Lys 
1               5                   10                  15      


Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser Arg Lys Asn 
            20                  25                  30          


Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln Val Thr His 
        35                  40                  45              


Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr Gln Gln Gln 
65                  70                  75                  80  


Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu Lys Tyr Pro 
                85                  90                  95      


Leu 
    


<210>  8
<211>  104
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6 SH2 2

<400>  8

Trp Tyr His Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln 
1               5                   10                  15      


Ala Lys Gly Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln 
            20                  25                  30          


Pro Gly Asp Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly 
        35                  40                  45              


Pro Gly Ser Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly 
    50                  55                  60                  


Gly Arg Tyr Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp 
65                  70                  75                  80  


Leu Val Glu His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala 
                85                  90                  95      


Phe Val Tyr Leu Arg Gln Pro Tyr 
            100                 


<210>  9
<211>  597
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Thr Ser Arg Arg Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala 
1               5                   10                  15      


Glu Asn Leu Leu Leu Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg 
            20                  25                  30          


Pro Ser Lys Ser Asn Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn 
        35                  40                  45              


Gly Ala Val Thr His Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp 
    50                  55                  60                  


Leu Tyr Gly Gly Glu Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr 
65                  70                  75                  80  


Tyr Met Glu His His Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile 
                85                  90                  95      


Glu Leu Lys Tyr Pro Leu Asn Cys Ala Asp Pro Thr Ser Glu Arg Trp 
            100                 105                 110         


Phe His Gly His Leu Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr Glu 
        115                 120                 125             


Lys Gly Lys His Gly Ser Phe Leu Val Arg Glu Ser Gln Ser His Pro 
    130                 135                 140                 


Gly Asp Phe Val Leu Ser Val Arg Thr Gly Asp Asp Lys Gly Glu Ser 
145                 150                 155                 160 


Asn Asp Gly Lys Ser Lys Val Thr His Val Met Ile Arg Cys Gln Glu 
                165                 170                 175     


Leu Lys Tyr Asp Val Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr Asp 
            180                 185                 190         


Leu Val Glu His Tyr Lys Lys Asn Pro Met Val Glu Thr Leu Gly Thr 
        195                 200                 205             


Val Leu Gln Leu Lys Gln Pro Leu Asn Thr Thr Arg Ile Asn Ala Ala 
    210                 215                 220                 


Glu Ile Glu Ser Arg Val Arg Glu Leu Ser Lys Leu Ala Glu Thr Thr 
225                 230                 235                 240 


Asp Lys Val Lys Gln Gly Phe Trp Glu Glu Phe Glu Thr Leu Gln Gln 
                245                 250                 255     


Gln Glu Cys Lys Leu Leu Tyr Ser Arg Lys Glu Gly Gln Arg Gln Glu 
            260                 265                 270         


Asn Lys Asn Lys Asn Arg Tyr Lys Asn Ile Leu Pro Phe Asp His Thr 
        275                 280                 285             


Arg Val Val Leu His Asp Gly Asp Pro Asn Glu Pro Val Ser Asp Tyr 
    290                 295                 300                 


Ile Asn Ala Asn Ile Ile Met Pro Glu Phe Glu Thr Lys Cys Asn Asn 
305                 310                 315                 320 


Ser Lys Pro Lys Lys Ser Tyr Ile Ala Thr Gln Gly Cys Leu Gln Asn 
                325                 330                 335     


Thr Val Asn Asp Phe Trp Arg Met Val Phe Gln Glu Asn Ser Arg Val 
            340                 345                 350         


Ile Val Met Thr Thr Lys Glu Val Glu Arg Gly Lys Ser Lys Cys Val 
        355                 360                 365             


Lys Tyr Trp Pro Asp Glu Tyr Ala Leu Lys Glu Tyr Gly Val Met Arg 
    370                 375                 380                 


Val Arg Asn Val Lys Glu Ser Ala Ala His Asp Tyr Thr Leu Arg Glu 
385                 390                 395                 400 


Leu Lys Leu Ser Lys Val Gly Gln Ala Leu Leu Gln Gly Asn Thr Glu 
                405                 410                 415     


Arg Thr Val Trp Gln Tyr His Phe Arg Thr Trp Pro Asp His Gly Val 
            420                 425                 430         


Pro Ser Asp Pro Gly Gly Val Leu Asp Phe Leu Glu Glu Val His His 
        435                 440                 445             


Lys Gln Glu Ser Ile Val Asp Ala Gly Pro Val Val Val His Cys Ser 
    450                 455                 460                 


Ala Gly Ile Gly Arg Thr Gly Thr Phe Ile Val Ile Asp Ile Leu Ile 
465                 470                 475                 480 


Asp Ile Ile Arg Glu Lys Gly Val Asp Cys Asp Ile Asp Val Pro Lys 
                485                 490                 495     


Thr Ile Gln Met Val Arg Ser Gln Arg Ser Gly Met Val Gln Thr Glu 
            500                 505                 510         


Ala Gln Tyr Arg Phe Ile Tyr Met Ala Val Gln His Tyr Ile Glu Thr 
        515                 520                 525             


Leu Gln Arg Arg Ile Glu Glu Glu Gln Lys Ser Lys Arg Lys Gly His 
    530                 535                 540                 


Glu Tyr Thr Asn Ile Lys Tyr Ser Leu Val Asp Gln Thr Ser Gly Asp 
545                 550                 555                 560 


Gln Ser Pro Leu Pro Pro Cys Thr Pro Thr Pro Pro Cys Ala Glu Met 
                565                 570                 575     


Arg Glu Asp Ser Ala Arg Val Tyr Glu Asn Val Gly Leu Met Gln Gln 
            580                 585                 590         


Gln Arg Ser Phe Arg 
        595         


<210>  10
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 first SH2 domain

<400>  10

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu 
    


<210>  11
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 second SH2 domain

<400>  11

Trp Phe His Gly His Leu Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr 
1               5                   10                  15      


Glu Lys Gly Lys His Gly Ser Phe Leu Val Arg Glu Ser Gln Ser His 
            20                  25                  30          


Pro Gly Asp Phe Val Leu Ser Val Arg Thr Gly Asp Asp Lys Gly Glu 
        35                  40                  45              


Ser Asn Asp Gly Lys Ser Lys Val Thr His Val Met Ile Arg Cys Gln 
    50                  55                  60                  


Glu Leu Lys Tyr Asp Val Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr 
65                  70                  75                  80  


Asp Leu Val Glu His Tyr Lys Lys Asn Pro Met Val Glu Thr Leu Gly 
                85                  90                  95      


Thr Val Leu Gln Leu Lys Gln Pro Leu 
            100                 105 


<210>  12
<211>  211
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 both SH2 domains

<400>  12

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu Asn Cys Ala Asp Pro Thr Ser Glu Arg Trp Phe His Gly His Leu 
            100                 105                 110         


Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr Glu Lys Gly Lys His Gly 
        115                 120                 125             


Ser Phe Leu Val Arg Glu Ser Gln Ser His Pro Gly Asp Phe Val Leu 
    130                 135                 140                 


Ser Val Arg Thr Gly Asp Asp Lys Gly Glu Ser Asn Asp Gly Lys Ser 
145                 150                 155                 160 


Lys Val Thr His Val Met Ile Arg Cys Gln Glu Leu Lys Tyr Asp Val 
                165                 170                 175     


Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr Asp Leu Val Glu His Tyr 
            180                 185                 190         


Lys Lys Asn Pro Met Val Glu Thr Leu Gly Thr Val Leu Gln Leu Lys 
        195                 200                 205             


Gln Pro Leu 
    210     


<210>  13
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3-zeta endodomain

<400>  13

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  14
<211>  754
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70-SH2 domain fused to a CD3-zeta endodomain

<400>  14

Met Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
1               5                   10                  15      


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            20                  25                  30          


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        35                  40                  45              


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
65                  70                  75                  80  


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                85                  90                  95      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            100                 105                 110         


Pro Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Gly Gly Gly Gly Ser Met Pro Asp Pro Ala Ala His Leu Pro 
    130                 135                 140                 


Phe Phe Tyr Gly Ser Ile Ser Arg Ala Glu Ala Glu Glu His Leu Lys 
145                 150                 155                 160 


Leu Ala Gly Met Ala Asp Gly Leu Phe Leu Leu Arg Gln Cys Leu Arg 
                165                 170                 175     


Ser Leu Gly Gly Tyr Val Leu Ser Leu Val His Asp Val Arg Phe His 
            180                 185                 190         


His Phe Pro Ile Glu Arg Gln Leu Asn Gly Thr Tyr Ala Ile Ala Gly 
        195                 200                 205             


Gly Lys Ala His Cys Gly Pro Ala Glu Leu Cys Glu Phe Tyr Ser Arg 
    210                 215                 220                 


Asp Pro Asp Gly Leu Pro Cys Asn Leu Arg Lys Pro Cys Asn Arg Pro 
225                 230                 235                 240 


Ser Gly Leu Glu Pro Gln Pro Gly Val Phe Asp Cys Leu Arg Asp Ala 
                245                 250                 255     


Met Val Arg Asp Tyr Val Arg Gln Thr Trp Lys Leu Glu Gly Glu Ala 
            260                 265                 270         


Leu Glu Gln Ala Ile Ile Ser Gln Ala Pro Gln Val Glu Lys Leu Ile 
        275                 280                 285             


Ala Thr Thr Ala His Glu Arg Met Pro Trp Tyr His Ser Ser Leu Thr 
    290                 295                 300                 


Arg Glu Glu Ala Glu Arg Lys Leu Tyr Ser Gly Ala Gln Thr Asp Gly 
305                 310                 315                 320 


Lys Phe Leu Leu Arg Pro Arg Lys Glu Gln Gly Thr Tyr Ala Leu Ser 
                325                 330                 335     


Leu Ile Tyr Gly Lys Thr Val Tyr His Tyr Leu Ile Ser Gln Asp Lys 
            340                 345                 350         


Ala Gly Lys Tyr Cys Ile Pro Glu Gly Thr Lys Phe Asp Thr Leu Trp 
        355                 360                 365             


Gln Leu Val Glu Tyr Leu Lys Leu Lys Ala Asp Gly Leu Ile Tyr Cys 
    370                 375                 380                 


Leu Lys Glu Ala Cys Pro Asn Ser Ser Ala Ser Asn Ala Ser Gly Ala 
385                 390                 395                 400 


Ala Ala Pro Thr Leu Pro Ala His Pro Ser Thr Leu Thr His Pro Gln 
                405                 410                 415     


Arg Arg Ile Asp Thr Leu Asn Ser Asp Gly Tyr Thr Pro Glu Pro Ala 
            420                 425                 430         


Arg Ile Thr Ser Pro Asp Lys Pro Arg Pro Met Pro Met Asp Thr Ser 
        435                 440                 445             


Val Tyr Glu Ser Pro Tyr Ser Asp Pro Glu Glu Leu Lys Asp Lys Lys 
    450                 455                 460                 


Leu Phe Leu Lys Arg Asp Asn Leu Leu Ile Ala Asp Ile Glu Leu Gly 
465                 470                 475                 480 


Cys Gly Asn Phe Gly Ser Val Arg Gln Gly Val Tyr Arg Met Arg Lys 
                485                 490                 495     


Lys Gln Ile Asp Val Ala Ile Lys Val Leu Lys Gln Gly Thr Glu Lys 
            500                 505                 510         


Ala Asp Thr Glu Glu Met Met Arg Glu Ala Gln Ile Met His Gln Leu 
        515                 520                 525             


Asp Asn Pro Tyr Ile Val Arg Leu Ile Gly Val Cys Gln Ala Glu Ala 
    530                 535                 540                 


Leu Met Leu Val Met Glu Met Ala Gly Gly Gly Pro Leu His Lys Phe 
545                 550                 555                 560 


Leu Val Gly Lys Arg Glu Glu Ile Pro Val Ser Asn Val Ala Glu Leu 
                565                 570                 575     


Leu His Gln Val Ser Met Gly Met Lys Tyr Leu Glu Glu Lys Asn Phe 
            580                 585                 590         


Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Leu Val Asn Arg His 
        595                 600                 605             


Tyr Ala Lys Ile Ser Asp Phe Gly Leu Ser Lys Ala Leu Gly Ala Asp 
    610                 615                 620                 


Asp Ser Tyr Tyr Thr Ala Arg Ser Ala Gly Lys Trp Pro Leu Lys Trp 
625                 630                 635                 640 


Tyr Ala Pro Glu Cys Ile Asn Phe Arg Lys Phe Ser Ser Arg Ser Asp 
                645                 650                 655     


Val Trp Ser Tyr Gly Val Thr Met Trp Glu Ala Leu Ser Tyr Gly Gln 
            660                 665                 670         


Lys Pro Tyr Lys Lys Met Lys Gly Pro Glu Val Met Ala Phe Ile Glu 
        675                 680                 685             


Gln Gly Lys Arg Met Glu Cys Pro Pro Glu Cys Pro Pro Glu Leu Tyr 
    690                 695                 700                 


Ala Leu Met Ser Asp Cys Trp Ile Tyr Lys Trp Glu Asp Arg Pro Asp 
705                 710                 715                 720 


Phe Leu Thr Val Glu Gln Arg Met Arg Ala Cys Tyr Tyr Ser Leu Ala 
                725                 730                 735     


Ser Lys Val Glu Gly Pro Pro Gly Ser Thr Gln Lys Ala Glu Ala Ala 
            740                 745                 750         


Cys Ala 
        


<210>  15
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDCD1 endodomain

<400>  15

Cys Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln 
1               5                   10                  15      


Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr 
            20                  25                  30          


Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val 
        35                  40                  45              


Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser 
    50                  55                  60                  


Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro 
65                  70                  75                  80  


Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro 
                85                  90                  95      


Leu 
    


<210>  16
<211>  141
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTLA4 endodomain

<400>  16

Lys Leu Gln Arg Arg Trp Lys Arg Thr Gln Ser Gln Gln Gly Leu Gln 
1               5                   10                  15      


Glu Asn Ser Ser Gly Gln Ser Phe Phe Val Arg Asn Lys Lys Val Arg 
            20                  25                  30          


Arg Ala Pro Leu Ser Glu Gly Pro His Ser Leu Gly Cys Tyr Asn Pro 
        35                  40                  45              


Met Met Glu Asp Gly Ile Ser Tyr Thr Thr Leu Arg Phe Pro Glu Met 
    50                  55                  60                  


Asn Ile Pro Arg Thr Gly Asp Ala Glu Ser Ser Glu Met Gln Arg Pro 
65                  70                  75                  80  


Pro Pro Asp Cys Asp Asp Thr Val Thr Tyr Ser Ala Leu His Lys Arg 
                85                  90                  95      


Gln Val Gly Asp Tyr Glu Asn Val Ile Pro Asp Phe Pro Glu Asp Glu 
            100                 105                 110         


Gly Ile His Tyr Ser Glu Leu Ile Gln Phe Gly Val Gly Glu Arg Pro 
        115                 120                 125             


Gln Ala Gln Glu Asn Val Asp Tyr Val Ile Leu Lys His 
    130                 135                 140     


<210>  17
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LILRB1 endodomain

<400>  17

Leu Arg His Arg Arg Gln Gly Lys His Trp Thr Ser Thr Gln Arg Lys 
1               5                   10                  15      


Ala Asp Phe Gln His Pro Ala Gly Ala Val Gly Pro Glu Pro Thr Asp 
            20                  25                  30          


Arg Gly Leu Gln Trp Arg Ser Ser Pro Ala Ala Asp Ala Gln Glu Glu 
        35                  40                  45              


Asn Leu Tyr Ala Ala Val Lys His Thr Gln Pro Glu Asp Gly Val Glu 
    50                  55                  60                  


Met Asp Thr Arg Ser Pro His Asp Glu Asp Pro Gln Ala Val Thr Tyr 
65                  70                  75                  80  


Ala Glu Val Lys His Ser Arg Pro Arg Arg Glu Met Ala Ser Pro Pro 
                85                  90                  95      


Ser Pro Leu Ser Gly Glu Phe Leu Asp Thr Lys Asp Arg Gln Ala Glu 
            100                 105                 110         


Glu Asp Arg Gln Met Asp Thr Glu Ala Ala Ala Ser Glu Ala Pro Gln 
        115                 120                 125             


Asp Val Thr Tyr Ala Gln Leu His Ser Leu Thr Leu Arg Arg Glu Ala 
    130                 135                 140                 


Thr Glu Pro Pro Pro Ser Gln Glu Gly Pro Ser Pro Ala Val Pro Ser 
145                 150                 155                 160 


Ile Tyr Ala Thr Leu Ala Ile His 
                165             


<210>  18
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LAIR1 endodomain

<400>  18

His Arg Gln Asn Gln Ile Lys Gln Gly Pro Pro Arg Ser Lys Asp Glu 
1               5                   10                  15      


Glu Gln Lys Pro Gln Gln Arg Pro Asp Leu Ala Val Asp Val Leu Glu 
            20                  25                  30          


Arg Thr Ala Asp Lys Ala Thr Val Asn Gly Leu Pro Glu Lys Asp Arg 
        35                  40                  45              


Glu Thr Asp Thr Ser Ala Leu Ala Ala Gly Ser Ser Gln Glu Val Thr 
    50                  55                  60                  


Tyr Ala Gln Leu Asp His Trp Ala Leu Thr Gln Arg Thr Ala Arg Ala 
65                  70                  75                  80  


Val Ser Pro Gln Ser Thr Lys Pro Met Ala Glu Ser Ile Thr Tyr Ala 
                85                  90                  95      


Ala Val Ala Arg His 
            100     


<210>  19
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CTLA4 endodomain

<400>  19

Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe Tyr 
1               5                   10                  15      


Ser Phe Leu Leu Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys Arg 
            20                  25                  30          


Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu Pro 
        35                  40                  45              


Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
    50                  55                  60          


<210>  20
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL1 endodomain

<400>  20

Gly Asn Ser Arg His Leu His Val Leu Ile Gly Thr Ser Val Val Ile 
1               5                   10                  15      


Ile Pro Phe Ala Ile Leu Leu Phe Phe Leu Leu His Arg Trp Cys Ala 
            20                  25                  30          


Asn Lys Lys Asn Ala Val Val Met Asp Gln Glu Pro Ala Gly Asn Arg 
        35                  40                  45              


Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln Glu Val Thr 
    50                  55                  60                  


Tyr Thr Gln Leu Asn His Cys Val Phe Thr Gln Arg Lys Ile Thr Arg 
65                  70                  75                  80  


Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Ile Ile Val Tyr Thr 
                85                  90                  95      


Glu Leu Pro Asn Ala Glu Ser Arg Ser Lys Val Val Ser Cys Pro 
            100                 105                 110     


<210>  21
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL4 endodomain

<400>  21

Gly Ile Ala Arg His Leu His Ala Val Ile Arg Tyr Ser Val Ala Ile 
1               5                   10                  15      


Ile Leu Phe Thr Ile Leu Pro Phe Phe Leu Leu His Arg Trp Cys Ser 
            20                  25                  30          


Lys Lys Lys Glu Asn Ala Ala Val Met Asn Gln Glu Pro Ala Gly His 
        35                  40                  45              


Arg Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln Glu Val 
    50                  55                  60                  


Thr Tyr Ala Gln Leu Asp His Cys Ile Phe Thr Gln Arg Lys Ile Thr 
65                  70                  75                  80  


Gly Pro Ser Gln Arg Ser Lys Arg Pro Ser Thr Asp Thr Ser Val Cys 
                85                  90                  95      


Ile Glu Leu Pro Asn Ala Glu Pro Arg Ala Leu Ser Pro Ala His Glu 
            100                 105                 110         


His His Ser Gln Ala Leu Met Gly Ser Ser Arg Glu Thr Thr Ala Leu 
        115                 120                 125             


Ser Gln Thr Gln Leu Ala Ser Ser Asn Val Pro Ala Ala Gly Ile 
    130                 135                 140             


<210>  22
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR2DL5 endodomain

<400>  22

Thr Gly Ile Arg Arg His Leu His Ile Leu Ile Gly Thr Ser Val Ala 
1               5                   10                  15      


Ile Ile Leu Phe Ile Ile Leu Phe Phe Phe Leu Leu His Cys Cys Cys 
            20                  25                  30          


Ser Asn Lys Lys Asn Ala Ala Val Met Asp Gln Glu Pro Ala Gly Asp 
        35                  40                  45              


Arg Thr Val Asn Arg Glu Asp Ser Asp Asp Gln Asp Pro Gln Glu Val 
    50                  55                  60                  


Thr Tyr Ala Gln Leu Asp His Cys Val Phe Thr Gln Thr Lys Ile Thr 
65                  70                  75                  80  


Ser Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Thr Met Tyr 
                85                  90                  95      


Met Glu Leu Pro Asn Ala Lys Pro Arg Ser Leu Ser Pro Ala His Lys 
            100                 105                 110         


His His Ser Gln Ala Leu Arg Gly Ser Ser Arg Glu Thr Thr Ala Leu 
        115                 120                 125             


Ser Gln Asn Arg Val Ala Ser Ser His Val Pro Ala Ala Gly Ile 
    130                 135                 140             


<210>  23
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR3DL1 endodomain

<400>  23

Lys Asp Pro Arg His Leu His Ile Leu Ile Gly Thr Ser Val Val Ile 
1               5                   10                  15      


Ile Leu Phe Ile Leu Leu Leu Phe Phe Leu Leu His Leu Trp Cys Ser 
            20                  25                  30          


Asn Lys Lys Asn Ala Ala Val Met Asp Gln Glu Pro Ala Gly Asn Arg 
        35                  40                  45              


Thr Ala Asn Ser Glu Asp Ser Asp Glu Gln Asp Pro Glu Glu Val Thr 
    50                  55                  60                  


Tyr Ala Gln Leu Asp His Cys Val Phe Thr Gln Arg Lys Ile Thr Arg 
65                  70                  75                  80  


Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Ile Leu Tyr Thr 
                85                  90                  95      


Glu Leu Pro Asn Ala Lys Pro Arg Ser Lys Val Val Ser Cys Pro 
            100                 105                 110     


<210>  24
<211>  97
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KIR3DL3 endodomain

<400>  24

Lys Asp Pro Gly Asn Ser Arg His Leu His Val Leu Ile Gly Thr Ser 
1               5                   10                  15      


Val Val Ile Ile Pro Phe Ala Ile Leu Leu Phe Phe Leu Leu His Arg 
            20                  25                  30          


Trp Cys Ala Asn Lys Lys Asn Ala Val Val Met Asp Gln Glu Pro Ala 
        35                  40                  45              


Gly Asn Arg Thr Val Asn Arg Glu Asp Ser Asp Glu Gln Asp Pro Gln 
    50                  55                  60                  


Glu Val Thr Tyr Ala Gln Leu Asn His Cys Val Phe Thr Gln Arg Lys 
65                  70                  75                  80  


Ile Thr Arg Pro Ser Gln Arg Pro Lys Thr Pro Pro Thr Asp Thr Ser 
                85                  90                  95      


Val 
    


<210>  25
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6-SH2 domain fused to a PD1 endodomain

<400>  25

Met Thr Gly Gln Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe 
1               5                   10                  15      


Ser Val Asp Tyr Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro 
            20                  25                  30          


Glu Pro Pro Val Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile 
        35                  40                  45              


Val Phe Pro Ser Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser 
    50                  55                  60                  


Ala Asp Gly Pro Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His 
65                  70                  75                  80  


Cys Ser Trp Pro Leu Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
                85                  90                  95      


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Val Arg Trp Phe His 
            100                 105                 110         


Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr Leu Leu Lys Gly Arg Gly 
        115                 120                 125             


Val His Gly Ser Phe Leu Ala Arg Pro Ser Arg Lys Asn Gln Gly Asp 
    130                 135                 140                 


Phe Ser Leu Ser Val Arg Val Gly Asp Gln Val Thr His Ile Arg Ile 
145                 150                 155                 160 


Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr Gly Gly Glu Lys Phe Ala 
                165                 170                 175     


Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr Gln Gln Gln Gly Val Leu 
            180                 185                 190         


Gln Asp Arg Asp Gly Thr Ile Ile His Leu Lys Tyr Pro Leu Asn Cys 
        195                 200                 205             


Ser Asp Pro Thr Ser Glu Arg Trp Tyr His Gly His Met Ser Gly Gly 
    210                 215                 220                 


Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly Glu Pro Trp Thr Phe Leu 
225                 230                 235                 240 


Val Arg Glu Ser Leu Ser Gln Pro Gly Asp Phe Val Leu Ser Val Leu 
                245                 250                 255     


Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser Pro Leu Arg Val Thr His 
            260                 265                 270         


Ile Lys Val Met Cys Glu Gly Gly Arg Tyr Thr Val Gly Gly Leu Glu 
        275                 280                 285             


Thr Phe Asp Ser Leu Thr Asp Leu Val Glu His Phe Lys Lys Thr Gly 
    290                 295                 300                 


Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr Leu Arg Gln Pro Tyr Tyr 
305                 310                 315                 320 


Ala Thr Arg Val Asn Ala Ala Asp Ile Glu Asn Arg Val Leu Glu Leu 
                325                 330                 335     


Asn Lys Lys Gln Glu Ser Glu Asp Thr Ala Lys Ala Gly Phe Trp Glu 
            340                 345                 350         


Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys Asn Leu His Gln Arg 
        355                 360                 365             


Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys Asn Arg Tyr Lys Asn 
    370                 375                 380                 


Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu Gln Gly Arg Asp Ser 
385                 390                 395                 400 


Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn Tyr Ile Lys Asn Gln 
                405                 410                 415     


Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr Ile Ala Ser Gln Gly 
            420                 425                 430         


Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln Met Ala Trp Gln Glu 
        435                 440                 445             


Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu Val Glu Lys Gly Arg 
    450                 455                 460                 


Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly Met Gln Arg Ala Tyr 
465                 470                 475                 480 


Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His Asp Thr Thr Glu Tyr 
                485                 490                 495     


Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp Asn Gly Asp Leu Ile 
            500                 505                 510         


Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser Trp Pro Asp His Gly Val 
        515                 520                 525             


Pro Ser Glu Pro Gly Gly Val Leu Ser Phe Leu Asp Gln Ile Asn Gln 
    530                 535                 540                 


Arg Gln Glu Ser Leu Pro His Ala Gly Pro Ile Ile Val His Cys Ser 
545                 550                 555                 560 


Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile Val Ile Asp Met Leu Met 
                565                 570                 575     


Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys Asp Ile Asp Ile Gln Lys 
            580                 585                 590         


Thr Ile Gln Met Val Arg Ala Gln Arg Ser Gly Met Val Gln Thr Glu 
        595                 600                 605             


Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile Ala Gln Phe Ile Glu Thr 
    610                 615                 620                 


Thr Lys Lys Lys Leu Glu Val Leu Gln Ser Gln Lys Gly Gln Glu Ser 
625                 630                 635                 640 


Glu Tyr Gly Asn Ile Thr Tyr Pro Pro Ala Met Lys Asn Ala His Ala 
                645                 650                 655     


Lys Ala Ser Arg Thr Ser Ser Lys His Lys Glu Asp Val Tyr Glu Asn 
            660                 665                 670         


Leu His Thr Lys Asn Lys Arg Glu Glu Lys Val Lys Lys Gln Arg Ser 
        675                 680                 685             


Ala Asp Lys Glu Lys Ser Lys Gly Ser Leu Lys Arg Lys 
    690                 695                 700     


<210>  26
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 kinase domain

<400>  26

Asp Pro Glu Glu Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn 
1               5                   10                  15      


Leu Leu Ile Ala Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val 
            20                  25                  30          


Arg Gln Gly Val Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile 
        35                  40                  45              


Lys Val Leu Lys Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met 
    50                  55                  60                  


Arg Glu Ala Gln Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg 
65                  70                  75                  80  


Leu Ile Gly Val Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met 
                85                  90                  95      


Ala Gly Gly Gly Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu 
            100                 105                 110         


Ile Pro Val Ser Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly 
        115                 120                 125             


Met Lys Tyr Leu Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala 
    130                 135                 140                 


Arg Asn Val Leu Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe 
145                 150                 155                 160 


Gly Leu Ser Lys Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg 
                165                 170                 175     


Ser Ala Gly Lys Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn 
            180                 185                 190         


Phe Arg Lys Phe Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr 
        195                 200                 205             


Met Trp Glu Ala Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys 
    210                 215                 220                 


Gly Pro Glu Val Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys 
225                 230                 235                 240 


Pro Pro Glu Cys Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp 
                245                 250                 255     


Ile Tyr Lys Trp Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg 
            260                 265                 270         


Met Arg Ala Cys Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro 
        275                 280                 285             


Gly Ser Thr Gln Lys Ala Glu Ala Ala Cys Ala 
    290                 295                 


<210>  27
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6 phosphatase domain

<400>  27

Phe Trp Glu Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys Asn Leu 
1               5                   10                  15      


His Gln Arg Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys Asn Arg 
            20                  25                  30          


Tyr Lys Asn Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu Gln Gly 
        35                  40                  45              


Arg Asp Ser Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn Tyr Ile 
    50                  55                  60                  


Lys Asn Gln Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr Ile Ala 
65                  70                  75                  80  


Ser Gln Gly Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln Met Ala 
                85                  90                  95      


Trp Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu Val Glu 
            100                 105                 110         


Lys Gly Arg Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly Met Gln 
        115                 120                 125             


Arg Ala Tyr Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His Asp Thr 
    130                 135                 140                 


Thr Glu Tyr Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp Asn Gly 
145                 150                 155                 160 


Asp Leu Ile Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser Trp Pro Asp 
                165                 170                 175     


His Gly Val Pro Ser Glu Pro Gly Gly Val Leu Ser Phe Leu Asp Gln 
            180                 185                 190         


Ile Asn Gln Arg Gln Glu Ser Leu Pro His Ala Gly Pro Ile Ile Val 
        195                 200                 205             


His Cys Ser Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile Val Ile Asp 
    210                 215                 220                 


Met Leu Met Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys Asp Ile Asp 
225                 230                 235                 240 


Ile Gln Lys Thr Ile Gln Met Val Arg Ala Gln Arg Ser Gly Met Val 
                245                 250                 255     


Gln Thr Glu Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile Ala Gln Phe 
            260                 265                 270         


Ile Glu Thr Thr Lys Lys Lys Leu 
        275                 280 


<210>  28
<211>  274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SHP-2 phosphatase domain

<400>  28

Trp Glu Glu Phe Glu Thr Leu Gln Gln Gln Glu Cys Lys Leu Leu Tyr 
1               5                   10                  15      


Ser Arg Lys Glu Gly Gln Arg Gln Glu Asn Lys Asn Lys Asn Arg Tyr 
            20                  25                  30          


Lys Asn Ile Leu Pro Phe Asp His Thr Arg Val Val Leu His Asp Gly 
        35                  40                  45              


Asp Pro Asn Glu Pro Val Ser Asp Tyr Ile Asn Ala Asn Ile Ile Met 
    50                  55                  60                  


Pro Glu Phe Glu Thr Lys Cys Asn Asn Ser Lys Pro Lys Lys Ser Tyr 
65                  70                  75                  80  


Ile Ala Thr Gln Gly Cys Leu Gln Asn Thr Val Asn Asp Phe Trp Arg 
                85                  90                  95      


Met Val Phe Gln Glu Asn Ser Arg Val Ile Val Met Thr Thr Lys Glu 
            100                 105                 110         


Val Glu Arg Gly Lys Ser Lys Cys Val Lys Tyr Trp Pro Asp Glu Tyr 
        115                 120                 125             


Ala Leu Lys Glu Tyr Gly Val Met Arg Val Arg Asn Val Lys Glu Ser 
    130                 135                 140                 


Ala Ala His Asp Tyr Thr Leu Arg Glu Leu Lys Leu Ser Lys Val Gly 
145                 150                 155                 160 


Gln Ala Leu Leu Gln Gly Asn Thr Glu Arg Thr Val Trp Gln Tyr His 
                165                 170                 175     


Phe Arg Thr Trp Pro Asp His Gly Val Pro Ser Asp Pro Gly Gly Val 
            180                 185                 190         


Leu Asp Phe Leu Glu Glu Val His His Lys Gln Glu Ser Ile Met Asp 
        195                 200                 205             


Ala Gly Pro Val Val Val His Cys Ser Ala Gly Ile Gly Arg Thr Gly 
    210                 215                 220                 


Thr Phe Ile Val Ile Asp Ile Leu Ile Asp Ile Ile Arg Glu Lys Gly 
225                 230                 235                 240 


Val Asp Cys Asp Ile Asp Val Pro Lys Thr Ile Gln Met Val Arg Ser 
                245                 250                 255     


Gln Arg Ser Gly Met Val Gln Thr Glu Ala Gln Tyr Arg Phe Ile Tyr 
            260                 265                 270         


Met Ala 
        


<210>  29
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6 SH2 domain fusion: ZAP70 kinase domain

<400>  29

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr Ser Gly Gly Gly Gly Ser Asp Pro Glu Glu 
    210                 215                 220                 


Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn Leu Leu Ile Ala 
225                 230                 235                 240 


Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val Arg Gln Gly Val 
                245                 250                 255     


Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile Lys Val Leu Lys 
            260                 265                 270         


Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met Arg Glu Ala Gln 
        275                 280                 285             


Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg Leu Ile Gly Val 
    290                 295                 300                 


Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met Ala Gly Gly Gly 
305                 310                 315                 320 


Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu Ile Pro Val Ser 
                325                 330                 335     


Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly Met Lys Tyr Leu 
            340                 345                 350         


Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala Arg Asn Val Leu 
        355                 360                 365             


Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe Gly Leu Ser Lys 
    370                 375                 380                 


Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg Ser Ala Gly Lys 
385                 390                 395                 400 


Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn Phe Arg Lys Phe 
                405                 410                 415     


Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr Met Trp Glu Ala 
            420                 425                 430         


Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys Gly Pro Glu Val 
        435                 440                 445             


Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys Pro Pro Glu Cys 
    450                 455                 460                 


Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp Ile Tyr Lys Trp 
465                 470                 475                 480 


Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg Met Arg Ala Cys 
                485                 490                 495     


Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro Gly Ser Thr Gln 
            500                 505                 510         


Lys Ala Glu Ala Ala Cys Ala 
        515                 


<210>  30
<211>  581
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 domain fusion: PTPN6 phosphatase domain

<400>  30

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ser Gly Gly Gly Gly Ser Gly Gly 
        275                 280                 285             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Phe Trp Glu 
    290                 295                 300                 


Glu Phe Glu Ser Leu Gln Lys Gln Glu Val Lys Asn Leu His Gln Arg 
305                 310                 315                 320 


Leu Glu Gly Gln Arg Pro Glu Asn Lys Gly Lys Asn Arg Tyr Lys Asn 
                325                 330                 335     


Ile Leu Pro Phe Asp His Ser Arg Val Ile Leu Gln Gly Arg Asp Ser 
            340                 345                 350         


Asn Ile Pro Gly Ser Asp Tyr Ile Asn Ala Asn Tyr Ile Lys Asn Gln 
        355                 360                 365             


Leu Leu Gly Pro Asp Glu Asn Ala Lys Thr Tyr Ile Ala Ser Gln Gly 
    370                 375                 380                 


Cys Leu Glu Ala Thr Val Asn Asp Phe Trp Gln Met Ala Trp Gln Glu 
385                 390                 395                 400 


Asn Ser Arg Val Ile Val Met Thr Thr Arg Glu Val Glu Lys Gly Arg 
                405                 410                 415     


Asn Lys Cys Val Pro Tyr Trp Pro Glu Val Gly Met Gln Arg Ala Tyr 
            420                 425                 430         


Gly Pro Tyr Ser Val Thr Asn Cys Gly Glu His Asp Thr Thr Glu Tyr 
        435                 440                 445             


Lys Leu Arg Thr Leu Gln Val Ser Pro Leu Asp Asn Gly Asp Leu Ile 
    450                 455                 460                 


Arg Glu Ile Trp His Tyr Gln Tyr Leu Ser Trp Pro Asp His Gly Val 
465                 470                 475                 480 


Pro Ser Glu Pro Gly Gly Val Leu Ser Phe Leu Asp Gln Ile Asn Gln 
                485                 490                 495     


Arg Gln Glu Ser Leu Pro His Ala Gly Pro Ile Ile Val His Cys Ser 
            500                 505                 510         


Ala Gly Ile Gly Arg Thr Gly Thr Ile Ile Val Ile Asp Met Leu Met 
        515                 520                 525             


Glu Asn Ile Ser Thr Lys Gly Leu Asp Cys Asp Ile Asp Ile Gln Lys 
    530                 535                 540                 


Thr Ile Gln Met Val Arg Ala Gln Arg Ser Gly Met Val Gln Thr Glu 
545                 550                 555                 560 


Ala Gln Tyr Lys Phe Ile Tyr Val Ala Ile Ala Gln Phe Ile Glu Thr 
                565                 570                 575     


Thr Lys Lys Lys Leu 
            580     


<210>  31
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 endodomain

<400>  31

Met Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
1               5                   10                  15      


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            20                  25                  30          


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40          


<210>  32
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB endodomain

<400>  32

Met Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
1               5                   10                  15      


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
            20                  25                  30          


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40              


<210>  33
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 endodomain

<400>  33

Met Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
1               5                   10                  15      


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
            20                  25                  30          


Thr Leu Ala Lys Ile 
        35          


<210>  34
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ICOS endodomain

<400>  34

Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn 
1               5                   10                  15      


Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg 
            20                  25                  30          


Leu Thr Asp Val Thr Leu 
        35              


<210>  35
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD27 endodomain

<400>  35

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  36
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTLA endodomain

<400>  36

Arg Arg His Gln Gly Lys Gln Asn Glu Leu Ser Asp Thr Ala Gly Arg 
1               5                   10                  15      


Glu Ile Asn Leu Val Asp Ala His Leu Lys Ser Glu Gln Thr Glu Ala 
            20                  25                  30          


Ser Thr Arg Gln Asn Ser Gln Val Leu Leu Ser Glu Thr Gly Ile Tyr 
        35                  40                  45              


Asp Asn Asp Pro Asp Leu Cys Phe Arg Met Gln Glu Gly Ser Glu Val 
    50                  55                  60                  


Tyr Ser Asn Pro Cys Leu Glu Glu Asn Lys Pro Gly Ile Val Tyr Ala 
65                  70                  75                  80  


Ser Leu Asn His Ser Val Ile Gly Pro Asn Ser Arg Leu Ala Arg Asn 
                85                  90                  95      


Val Lys Glu Ala Pro Thr Glu Tyr Ala Ser Ile Cys Val Arg Ser 
            100                 105                 110     


<210>  37
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD30 endodomain

<400>  37

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  38
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GITR endodomain

<400>  38

Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met Trp Pro 
1               5                   10                  15      


Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu Asp Ala 
            20                  25                  30          


Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu 
        35                  40                  45              


Glu Lys Gly Arg Leu Gly Asp Leu Trp Val 
    50                  55              


<210>  39
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HVEM endodomain

<400>  39

Cys Val Lys Arg Arg Lys Pro Arg Gly Asp Val Val Lys Val Ile Val 
1               5                   10                  15      


Ser Val Gln Arg Lys Arg Gln Glu Ala Glu Gly Glu Ala Thr Val Ile 
            20                  25                  30          


Glu Ala Leu Gln Ala Pro Pro Asp Val Thr Thr Val Ala Val Glu Glu 
        35                  40                  45              


Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro Asn His 
    50                  55                  60  


<210>  40
<211>  682
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 endodomain fused to amino-terminus of full-length ZAP

<400>  40

Met Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
1               5                   10                  15      


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            20                  25                  30          


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ser Gly Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met 
    50                  55                  60                  


Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser Arg 
65                  70                  75                  80  


Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly Leu 
                85                  90                  95      


Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu Ser 
            100                 105                 110         


Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln Leu 
        115                 120                 125             


Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro Ala 
    130                 135                 140                 


Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys Asn 
145                 150                 155                 160 


Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro Gly 
                165                 170                 175     


Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg Gln 
            180                 185                 190         


Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser Gln 
        195                 200                 205             


Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg Met 
    210                 215                 220                 


Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys Leu 
225                 230                 235                 240 


Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg Lys 
                245                 250                 255     


Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val Tyr 
            260                 265                 270         


His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro Glu 
        275                 280                 285             


Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys Leu 
    290                 295                 300                 


Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn Ser 
305                 310                 315                 320 


Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala His 
                325                 330                 335     


Pro Ser Thr Leu Thr His Pro Gln Arg Arg Ile Asp Thr Leu Asn Ser 
            340                 345                 350         


Asp Gly Tyr Thr Pro Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys Pro 
        355                 360                 365             


Arg Pro Met Pro Met Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser Asp 
    370                 375                 380                 


Pro Glu Glu Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn Leu 
385                 390                 395                 400 


Leu Ile Ala Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val Arg 
                405                 410                 415     


Gln Gly Val Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile Lys 
            420                 425                 430         


Val Leu Lys Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met Arg 
        435                 440                 445             


Glu Ala Gln Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg Leu 
    450                 455                 460                 


Ile Gly Val Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met Ala 
465                 470                 475                 480 


Gly Gly Gly Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu Ile 
                485                 490                 495     


Pro Val Ser Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly Met 
            500                 505                 510         


Lys Tyr Leu Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala Arg 
        515                 520                 525             


Asn Val Leu Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe Gly 
    530                 535                 540                 


Leu Ser Lys Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg Ser 
545                 550                 555                 560 


Ala Gly Lys Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn Phe 
                565                 570                 575     


Arg Lys Phe Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr Met 
            580                 585                 590         


Trp Glu Ala Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys Gly 
        595                 600                 605             


Pro Glu Val Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys Pro 
    610                 615                 620                 


Pro Glu Cys Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp Ile 
625                 630                 635                 640 


Tyr Lys Trp Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg Met 
                645                 650                 655     


Arg Ala Cys Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro Gly 
            660                 665                 670         


Ser Thr Gln Lys Ala Glu Ala Ala Cys Ala 
        675                 680         


<210>  41
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB endodomain fused to amino-terminus of full-length ZAP

<400>  41

Met Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
1               5                   10                  15      


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
            20                  25                  30          


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Ser Gly Gly Gly Gly 
        35                  40                  45              


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55                  60                  


Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
65                  70                  75                  80  


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
                85                  90                  95      


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
            100                 105                 110         


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
        115                 120                 125             


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
    130                 135                 140                 


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
145                 150                 155                 160 


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
                165                 170                 175     


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
            180                 185                 190         


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
        195                 200                 205             


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
    210                 215                 220                 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
225                 230                 235                 240 


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
                245                 250                 255     


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
            260                 265                 270         


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
        275                 280                 285             


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
    290                 295                 300                 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
305                 310                 315                 320 


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
                325                 330                 335     


His Pro Ser Thr Leu Thr His Pro Gln Arg Arg Ile Asp Thr Leu Asn 
            340                 345                 350         


Ser Asp Gly Tyr Thr Pro Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys 
        355                 360                 365             


Pro Arg Pro Met Pro Met Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser 
    370                 375                 380                 


Asp Pro Glu Glu Leu Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn 
385                 390                 395                 400 


Leu Leu Ile Ala Asp Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val 
                405                 410                 415     


Arg Gln Gly Val Tyr Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile 
            420                 425                 430         


Lys Val Leu Lys Gln Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met 
        435                 440                 445             


Arg Glu Ala Gln Ile Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg 
    450                 455                 460                 


Leu Ile Gly Val Cys Gln Ala Glu Ala Leu Met Leu Val Met Glu Met 
465                 470                 475                 480 


Ala Gly Gly Gly Pro Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu 
                485                 490                 495     


Ile Pro Val Ser Asn Val Ala Glu Leu Leu His Gln Val Ser Met Gly 
            500                 505                 510         


Met Lys Tyr Leu Glu Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala 
        515                 520                 525             


Arg Asn Val Leu Leu Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe 
    530                 535                 540                 


Gly Leu Ser Lys Ala Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg 
545                 550                 555                 560 


Ser Ala Gly Lys Trp Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn 
                565                 570                 575     


Phe Arg Lys Phe Ser Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr 
            580                 585                 590         


Met Trp Glu Ala Leu Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys 
        595                 600                 605             


Gly Pro Glu Val Met Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys 
    610                 615                 620                 


Pro Pro Glu Cys Pro Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp 
625                 630                 635                 640 


Ile Tyr Lys Trp Glu Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg 
                645                 650                 655     


Met Arg Ala Cys Tyr Tyr Ser Leu Ala Ser Lys Val Glu Gly Pro Pro 
            660                 665                 670         


Gly Ser Thr Gln Lys Ala Glu Ala Ala Cys Ala 
        675                 680             


<210>  42
<211>  677
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 endodomain fused to amino-terminus of full-length ZAP

<400>  42

Met Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
1               5                   10                  15      


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
            20                  25                  30          


Thr Leu Ala Lys Ile Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Pro Asp Pro Ala Ala 
    50                  55                  60                  


His Leu Pro Phe Phe Tyr Gly Ser Ile Ser Arg Ala Glu Ala Glu Glu 
65                  70                  75                  80  


His Leu Lys Leu Ala Gly Met Ala Asp Gly Leu Phe Leu Leu Arg Gln 
                85                  90                  95      


Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu Ser Leu Val His Asp Val 
            100                 105                 110         


Arg Phe His His Phe Pro Ile Glu Arg Gln Leu Asn Gly Thr Tyr Ala 
        115                 120                 125             


Ile Ala Gly Gly Lys Ala His Cys Gly Pro Ala Glu Leu Cys Glu Phe 
    130                 135                 140                 


Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys Asn Leu Arg Lys Pro Cys 
145                 150                 155                 160 


Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro Gly Val Phe Asp Cys Leu 
                165                 170                 175     


Arg Asp Ala Met Val Arg Asp Tyr Val Arg Gln Thr Trp Lys Leu Glu 
            180                 185                 190         


Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser Gln Ala Pro Gln Val Glu 
        195                 200                 205             


Lys Leu Ile Ala Thr Thr Ala His Glu Arg Met Pro Trp Tyr His Ser 
    210                 215                 220                 


Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys Leu Tyr Ser Gly Ala Gln 
225                 230                 235                 240 


Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg Lys Glu Gln Gly Thr Tyr 
                245                 250                 255     


Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val Tyr His Tyr Leu Ile Ser 
            260                 265                 270         


Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro Glu Gly Thr Lys Phe Asp 
        275                 280                 285             


Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys Leu Lys Ala Asp Gly Leu 
    290                 295                 300                 


Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn Ser Ser Ala Ser Asn Ala 
305                 310                 315                 320 


Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala His Pro Ser Thr Leu Thr 
                325                 330                 335     


His Pro Gln Arg Arg Ile Asp Thr Leu Asn Ser Asp Gly Tyr Thr Pro 
            340                 345                 350         


Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys Pro Arg Pro Met Pro Met 
        355                 360                 365             


Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser Asp Pro Glu Glu Leu Lys 
    370                 375                 380                 


Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn Leu Leu Ile Ala Asp Ile 
385                 390                 395                 400 


Glu Leu Gly Cys Gly Asn Phe Gly Ser Val Arg Gln Gly Val Tyr Arg 
                405                 410                 415     


Met Arg Lys Lys Gln Ile Asp Val Ala Ile Lys Val Leu Lys Gln Gly 
            420                 425                 430         


Thr Glu Lys Ala Asp Thr Glu Glu Met Met Arg Glu Ala Gln Ile Met 
        435                 440                 445             


His Gln Leu Asp Asn Pro Tyr Ile Val Arg Leu Ile Gly Val Cys Gln 
    450                 455                 460                 


Ala Glu Ala Leu Met Leu Val Met Glu Met Ala Gly Gly Gly Pro Leu 
465                 470                 475                 480 


His Lys Phe Leu Val Gly Lys Arg Glu Glu Ile Pro Val Ser Asn Val 
                485                 490                 495     


Ala Glu Leu Leu His Gln Val Ser Met Gly Met Lys Tyr Leu Glu Glu 
            500                 505                 510         


Lys Asn Phe Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Leu Val 
        515                 520                 525             


Asn Arg His Tyr Ala Lys Ile Ser Asp Phe Gly Leu Ser Lys Ala Leu 
    530                 535                 540                 


Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg Ser Ala Gly Lys Trp Pro 
545                 550                 555                 560 


Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn Phe Arg Lys Phe Ser Ser 
                565                 570                 575     


Arg Ser Asp Val Trp Ser Tyr Gly Val Thr Met Trp Glu Ala Leu Ser 
            580                 585                 590         


Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys Gly Pro Glu Val Met Ala 
        595                 600                 605             


Phe Ile Glu Gln Gly Lys Arg Met Glu Cys Pro Pro Glu Cys Pro Pro 
    610                 615                 620                 


Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp Ile Tyr Lys Trp Glu Asp 
625                 630                 635                 640 


Arg Pro Asp Phe Leu Thr Val Glu Gln Arg Met Arg Ala Cys Tyr Tyr 
                645                 650                 655     


Ser Leu Ala Ser Lys Val Glu Gly Pro Pro Gly Ser Thr Gln Lys Ala 
            660                 665                 670         


Glu Ala Ala Cys Ala 
        675         


<210>  43
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 endodomain fused to the amino-terminus of PTPN6 SH2 domain

<400>  43

Met Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
1               5                   10                  15      


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
            20                  25                  30          


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ser Gly Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met 
    50                  55                  60                  


Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr Leu 
65                  70                  75                  80  


Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser Arg 
                85                  90                  95      


Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln Val 
            100                 105                 110         


Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr Gly 
        115                 120                 125             


Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr Gln 
    130                 135                 140                 


Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu Lys 
145                 150                 155                 160 


Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His Gly 
                165                 170                 175     


His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly Glu 
            180                 185                 190         


Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp Phe 
        195                 200                 205             


Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser Pro 
    210                 215                 220                 


Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr Thr 
225                 230                 235                 240 


Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu His 
                245                 250                 255     


Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr Leu 
            260                 265                 270         


Arg Gln Pro Tyr 
        275     


<210>  44
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  41BB endodomain fused to the amino-terminus of PTPN6 SH2 domain

<400>  44

Met Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
1               5                   10                  15      


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
            20                  25                  30          


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Ser Gly Gly Gly Gly 
        35                  40                  45              


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55                  60                  


Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
65                  70                  75                  80  


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
                85                  90                  95      


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
            100                 105                 110         


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
        115                 120                 125             


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
    130                 135                 140                 


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
145                 150                 155                 160 


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
                165                 170                 175     


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
            180                 185                 190         


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
        195                 200                 205             


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
    210                 215                 220                 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
225                 230                 235                 240 


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
                245                 250                 255     


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
            260                 265                 270         


Leu Arg Gln Pro Tyr 
        275         


<210>  45
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OX40 endodomain fused to the amino-terminus of PTPN6 SH2 domain

<400>  45

Met Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
1               5                   10                  15      


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
            20                  25                  30          


Thr Leu Ala Lys Ile Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45              


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Val Arg Trp Phe His 
    50                  55                  60                  


Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr Leu Leu Lys Gly Arg Gly 
65                  70                  75                  80  


Val His Gly Ser Phe Leu Ala Arg Pro Ser Arg Lys Asn Gln Gly Asp 
                85                  90                  95      


Phe Ser Leu Ser Val Arg Val Gly Asp Gln Val Thr His Ile Arg Ile 
            100                 105                 110         


Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr Gly Gly Glu Lys Phe Ala 
        115                 120                 125             


Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr Gln Gln Gln Gly Val Leu 
    130                 135                 140                 


Gln Asp Arg Asp Gly Thr Ile Ile His Leu Lys Tyr Pro Leu Asn Cys 
145                 150                 155                 160 


Ser Asp Pro Thr Ser Glu Arg Trp Tyr His Gly His Met Ser Gly Gly 
                165                 170                 175     


Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly Glu Pro Trp Thr Phe Leu 
            180                 185                 190         


Val Arg Glu Ser Leu Ser Gln Pro Gly Asp Phe Val Leu Ser Val Leu 
        195                 200                 205             


Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser Pro Leu Arg Val Thr His 
    210                 215                 220                 


Ile Lys Val Met Cys Glu Gly Gly Arg Tyr Thr Val Gly Gly Leu Glu 
225                 230                 235                 240 


Thr Phe Asp Ser Leu Thr Asp Leu Val Glu His Phe Lys Lys Thr Gly 
                245                 250                 255     


Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr Leu Arg Gln Pro Tyr 
            260                 265                 270     


<210>  46
<211>  350
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Akt kinase domain

<400>  46

Ala Glu Glu Met Glu Val Ser Leu Ala Lys Pro Lys His Arg Val Thr 
1               5                   10                  15      


Met Asn Glu Phe Glu Tyr Leu Lys Leu Leu Gly Lys Gly Thr Phe Gly 
            20                  25                  30          


Lys Val Ile Leu Val Lys Glu Lys Ala Thr Gly Arg Tyr Tyr Ala Met 
        35                  40                  45              


Lys Ile Leu Lys Lys Glu Val Ile Val Ala Lys Asp Glu Val Ala His 
    50                  55                  60                  


Thr Leu Thr Glu Asn Arg Val Leu Gln Asn Ser Arg His Pro Phe Leu 
65                  70                  75                  80  


Thr Ala Leu Lys Tyr Ser Phe Gln Thr His Asp Arg Leu Cys Phe Val 
                85                  90                  95      


Met Glu Tyr Ala Asn Gly Gly Glu Leu Phe Phe His Leu Ser Arg Glu 
            100                 105                 110         


Arg Val Phe Ser Glu Asp Arg Ala Arg Phe Tyr Gly Ala Glu Ile Val 
        115                 120                 125             


Ser Ala Leu Asp Tyr Leu His Ser Glu Lys Asn Val Val Tyr Arg Asp 
    130                 135                 140                 


Leu Lys Leu Glu Asn Leu Met Leu Asp Lys Asp Gly His Ile Lys Ile 
145                 150                 155                 160 


Thr Asp Phe Gly Leu Cys Lys Glu Gly Ile Lys Asp Gly Ala Thr Met 
                165                 170                 175     


Lys Thr Phe Cys Gly Thr Pro Glu Tyr Leu Ala Pro Glu Val Leu Glu 
            180                 185                 190         


Asp Asn Asp Tyr Gly Arg Ala Val Asp Trp Trp Gly Leu Gly Val Val 
        195                 200                 205             


Met Tyr Glu Met Met Cys Gly Arg Leu Pro Phe Tyr Asn Gln Asp His 
    210                 215                 220                 


Glu Lys Leu Phe Glu Leu Ile Leu Met Glu Glu Ile Arg Phe Pro Arg 
225                 230                 235                 240 


Thr Leu Gly Pro Glu Ala Lys Ser Leu Leu Ser Gly Leu Leu Lys Lys 
                245                 250                 255     


Asp Pro Lys Gln Arg Leu Gly Gly Gly Ser Glu Asp Ala Lys Glu Ile 
            260                 265                 270         


Met Gln His Arg Phe Phe Ala Gly Ile Val Trp Gln His Val Tyr Glu 
        275                 280                 285             


Lys Lys Leu Ser Pro Pro Phe Lys Pro Gln Val Thr Ser Glu Thr Asp 
    290                 295                 300                 


Thr Arg Tyr Phe Asp Glu Glu Phe Thr Ala Gln Met Ile Thr Ile Thr 
305                 310                 315                 320 


Pro Pro Asp Gln Asp Asp Ser Met Glu Cys Val Asp Ser Glu Arg Arg 
                325                 330                 335     


Pro His Phe Pro Gln Phe Ser Tyr Ser Ala Ser Gly Thr Ala 
            340                 345                 350 


<210>  47
<211>  630
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70-SH2 domain fused directly to an Akt kinase domain

<400>  47

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ala Glu Glu Met Glu Val Ser Leu 
        275                 280                 285             


Ala Lys Pro Lys His Arg Val Thr Met Asn Glu Phe Glu Tyr Leu Lys 
    290                 295                 300                 


Leu Leu Gly Lys Gly Thr Phe Gly Lys Val Ile Leu Val Lys Glu Lys 
305                 310                 315                 320 


Ala Thr Gly Arg Tyr Tyr Ala Met Lys Ile Leu Lys Lys Glu Val Ile 
                325                 330                 335     


Val Ala Lys Asp Glu Val Ala His Thr Leu Thr Glu Asn Arg Val Leu 
            340                 345                 350         


Gln Asn Ser Arg His Pro Phe Leu Thr Ala Leu Lys Tyr Ser Phe Gln 
        355                 360                 365             


Thr His Asp Arg Leu Cys Phe Val Met Glu Tyr Ala Asn Gly Gly Glu 
    370                 375                 380                 


Leu Phe Phe His Leu Ser Arg Glu Arg Val Phe Ser Glu Asp Arg Ala 
385                 390                 395                 400 


Arg Phe Tyr Gly Ala Glu Ile Val Ser Ala Leu Asp Tyr Leu His Ser 
                405                 410                 415     


Glu Lys Asn Val Val Tyr Arg Asp Leu Lys Leu Glu Asn Leu Met Leu 
            420                 425                 430         


Asp Lys Asp Gly His Ile Lys Ile Thr Asp Phe Gly Leu Cys Lys Glu 
        435                 440                 445             


Gly Ile Lys Asp Gly Ala Thr Met Lys Thr Phe Cys Gly Thr Pro Glu 
    450                 455                 460                 


Tyr Leu Ala Pro Glu Val Leu Glu Asp Asn Asp Tyr Gly Arg Ala Val 
465                 470                 475                 480 


Asp Trp Trp Gly Leu Gly Val Val Met Tyr Glu Met Met Cys Gly Arg 
                485                 490                 495     


Leu Pro Phe Tyr Asn Gln Asp His Glu Lys Leu Phe Glu Leu Ile Leu 
            500                 505                 510         


Met Glu Glu Ile Arg Phe Pro Arg Thr Leu Gly Pro Glu Ala Lys Ser 
        515                 520                 525             


Leu Leu Ser Gly Leu Leu Lys Lys Asp Pro Lys Gln Arg Leu Gly Gly 
    530                 535                 540                 


Gly Ser Glu Asp Ala Lys Glu Ile Met Gln His Arg Phe Phe Ala Gly 
545                 550                 555                 560 


Ile Val Trp Gln His Val Tyr Glu Lys Lys Leu Ser Pro Pro Phe Lys 
                565                 570                 575     


Pro Gln Val Thr Ser Glu Thr Asp Thr Arg Tyr Phe Asp Glu Glu Phe 
            580                 585                 590         


Thr Ala Gln Met Ile Thr Ile Thr Pro Pro Asp Gln Asp Asp Ser Met 
        595                 600                 605             


Glu Cys Val Asp Ser Glu Arg Arg Pro His Phe Pro Gln Phe Ser Tyr 
    610                 615                 620                 


Ser Ala Ser Gly Thr Ala 
625                 630 


<210>  48
<211>  651
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70-SH2 domain fused to an Akt kinase domain via a linker

<400>  48

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ser Gly Gly Gly Gly Ser Gly Gly 
        275                 280                 285             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Glu Glu 
    290                 295                 300                 


Met Glu Val Ser Leu Ala Lys Pro Lys His Arg Val Thr Met Asn Glu 
305                 310                 315                 320 


Phe Glu Tyr Leu Lys Leu Leu Gly Lys Gly Thr Phe Gly Lys Val Ile 
                325                 330                 335     


Leu Val Lys Glu Lys Ala Thr Gly Arg Tyr Tyr Ala Met Lys Ile Leu 
            340                 345                 350         


Lys Lys Glu Val Ile Val Ala Lys Asp Glu Val Ala His Thr Leu Thr 
        355                 360                 365             


Glu Asn Arg Val Leu Gln Asn Ser Arg His Pro Phe Leu Thr Ala Leu 
    370                 375                 380                 


Lys Tyr Ser Phe Gln Thr His Asp Arg Leu Cys Phe Val Met Glu Tyr 
385                 390                 395                 400 


Ala Asn Gly Gly Glu Leu Phe Phe His Leu Ser Arg Glu Arg Val Phe 
                405                 410                 415     


Ser Glu Asp Arg Ala Arg Phe Tyr Gly Ala Glu Ile Val Ser Ala Leu 
            420                 425                 430         


Asp Tyr Leu His Ser Glu Lys Asn Val Val Tyr Arg Asp Leu Lys Leu 
        435                 440                 445             


Glu Asn Leu Met Leu Asp Lys Asp Gly His Ile Lys Ile Thr Asp Phe 
    450                 455                 460                 


Gly Leu Cys Lys Glu Gly Ile Lys Asp Gly Ala Thr Met Lys Thr Phe 
465                 470                 475                 480 


Cys Gly Thr Pro Glu Tyr Leu Ala Pro Glu Val Leu Glu Asp Asn Asp 
                485                 490                 495     


Tyr Gly Arg Ala Val Asp Trp Trp Gly Leu Gly Val Val Met Tyr Glu 
            500                 505                 510         


Met Met Cys Gly Arg Leu Pro Phe Tyr Asn Gln Asp His Glu Lys Leu 
        515                 520                 525             


Phe Glu Leu Ile Leu Met Glu Glu Ile Arg Phe Pro Arg Thr Leu Gly 
    530                 535                 540                 


Pro Glu Ala Lys Ser Leu Leu Ser Gly Leu Leu Lys Lys Asp Pro Lys 
545                 550                 555                 560 


Gln Arg Leu Gly Gly Gly Ser Glu Asp Ala Lys Glu Ile Met Gln His 
                565                 570                 575     


Arg Phe Phe Ala Gly Ile Val Trp Gln His Val Tyr Glu Lys Lys Leu 
            580                 585                 590         


Ser Pro Pro Phe Lys Pro Gln Val Thr Ser Glu Thr Asp Thr Arg Tyr 
        595                 600                 605             


Phe Asp Glu Glu Phe Thr Ala Gln Met Ile Thr Ile Thr Pro Pro Asp 
    610                 615                 620                 


Gln Asp Asp Ser Met Glu Cys Val Asp Ser Glu Arg Arg Pro His Phe 
625                 630                 635                 640 


Pro Gln Phe Ser Tyr Ser Ala Ser Gly Thr Ala 
                645                 650     


<210>  49
<211>  630
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 mutated to be non-functional and fused to an Akt kinase 
       domain

<400>  49

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Lys Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ala Glu Glu Met Glu Val Ser Leu 
        275                 280                 285             


Ala Lys Pro Lys His Arg Val Thr Met Asn Glu Phe Glu Tyr Leu Lys 
    290                 295                 300                 


Leu Leu Gly Lys Gly Thr Phe Gly Lys Val Ile Leu Val Lys Glu Lys 
305                 310                 315                 320 


Ala Thr Gly Arg Tyr Tyr Ala Met Lys Ile Leu Lys Lys Glu Val Ile 
                325                 330                 335     


Val Ala Lys Asp Glu Val Ala His Thr Leu Thr Glu Asn Arg Val Leu 
            340                 345                 350         


Gln Asn Ser Arg His Pro Phe Leu Thr Ala Leu Lys Tyr Ser Phe Gln 
        355                 360                 365             


Thr His Asp Arg Leu Cys Phe Val Met Glu Tyr Ala Asn Gly Gly Glu 
    370                 375                 380                 


Leu Phe Phe His Leu Ser Arg Glu Arg Val Phe Ser Glu Asp Arg Ala 
385                 390                 395                 400 


Arg Phe Tyr Gly Ala Glu Ile Val Ser Ala Leu Asp Tyr Leu His Ser 
                405                 410                 415     


Glu Lys Asn Val Val Tyr Arg Asp Leu Lys Leu Glu Asn Leu Met Leu 
            420                 425                 430         


Asp Lys Asp Gly His Ile Lys Ile Thr Asp Phe Gly Leu Cys Lys Glu 
        435                 440                 445             


Gly Ile Lys Asp Gly Ala Thr Met Lys Thr Phe Cys Gly Thr Pro Glu 
    450                 455                 460                 


Tyr Leu Ala Pro Glu Val Leu Glu Asp Asn Asp Tyr Gly Arg Ala Val 
465                 470                 475                 480 


Asp Trp Trp Gly Leu Gly Val Val Met Tyr Glu Met Met Cys Gly Arg 
                485                 490                 495     


Leu Pro Phe Tyr Asn Gln Asp His Glu Lys Leu Phe Glu Leu Ile Leu 
            500                 505                 510         


Met Glu Glu Ile Arg Phe Pro Arg Thr Leu Gly Pro Glu Ala Lys Ser 
        515                 520                 525             


Leu Leu Ser Gly Leu Leu Lys Lys Asp Pro Lys Gln Arg Leu Gly Gly 
    530                 535                 540                 


Gly Ser Glu Asp Ala Lys Glu Ile Met Gln His Arg Phe Phe Ala Gly 
545                 550                 555                 560 


Ile Val Trp Gln His Val Tyr Glu Lys Lys Leu Ser Pro Pro Phe Lys 
                565                 570                 575     


Pro Gln Val Thr Ser Glu Thr Asp Thr Arg Tyr Phe Asp Glu Glu Phe 
            580                 585                 590         


Thr Ala Gln Met Ile Thr Ile Thr Pro Pro Asp Gln Asp Asp Ser Met 
        595                 600                 605             


Glu Cys Val Asp Ser Glu Arg Arg Pro His Phe Pro Gln Phe Ser Tyr 
    610                 615                 620                 


Ser Ala Ser Gly Thr Ala 
625                 630 


<210>  50
<211>  590
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Kinase containing domain of JAK2

<400>  50

Arg Asn Glu Asp Leu Ile Phe Asn Glu Ser Leu Gly Gln Gly Thr Phe 
1               5                   10                  15      


Thr Lys Ile Phe Lys Gly Val Arg Arg Glu Val Gly Asp Tyr Gly Gln 
            20                  25                  30          


Leu His Glu Thr Glu Val Leu Leu Lys Val Leu Asp Lys Ala His Arg 
        35                  40                  45              


Asn Tyr Ser Glu Ser Phe Phe Glu Ala Ala Ser Met Met Ser Lys Leu 
    50                  55                  60                  


Ser His Lys His Leu Val Leu Asn Tyr Gly Val Cys Val Cys Gly Asp 
65                  70                  75                  80  


Glu Asn Ile Leu Val Gln Glu Phe Val Lys Phe Gly Ser Leu Asp Thr 
                85                  90                  95      


Tyr Leu Lys Lys Asn Lys Asn Cys Ile Asn Ile Leu Trp Lys Leu Glu 
            100                 105                 110         


Val Ala Lys Gln Leu Ala Trp Ala Met His Phe Leu Glu Glu Asn Thr 
        115                 120                 125             


Leu Ile His Gly Asn Val Cys Ala Lys Asn Ile Leu Leu Ile Arg Glu 
    130                 135                 140                 


Glu Asp Arg Lys Thr Gly Asn Pro Pro Phe Ile Lys Leu Ser Asp Pro 
145                 150                 155                 160 


Gly Ile Ser Ile Thr Val Leu Pro Lys Asp Ile Leu Gln Glu Arg Ile 
                165                 170                 175     


Pro Trp Val Pro Pro Glu Cys Ile Glu Asn Pro Lys Asn Leu Asn Leu 
            180                 185                 190         


Ala Thr Asp Lys Trp Ser Phe Gly Thr Thr Leu Trp Glu Ile Cys Ser 
        195                 200                 205             


Gly Gly Asp Lys Pro Leu Ser Ala Leu Asp Ser Gln Arg Lys Leu Gln 
    210                 215                 220                 


Phe Tyr Glu Asp Arg His Gln Leu Pro Ala Pro Lys Trp Ala Glu Leu 
225                 230                 235                 240 


Ala Asn Leu Ile Asn Asn Cys Met Asp Tyr Glu Pro Asp Phe Arg Pro 
                245                 250                 255     


Ser Phe Arg Ala Ile Ile Arg Asp Leu Asn Ser Leu Phe Thr Pro Asp 
            260                 265                 270         


Tyr Glu Leu Leu Thr Glu Asn Asp Met Leu Pro Asn Met Arg Ile Gly 
        275                 280                 285             


Ala Leu Gly Phe Ser Gly Ala Phe Glu Asp Arg Asp Pro Thr Gln Phe 
    290                 295                 300                 


Glu Glu Arg His Leu Lys Phe Leu Gln Gln Leu Gly Lys Gly Asn Phe 
305                 310                 315                 320 


Gly Ser Val Glu Met Cys Arg Tyr Asp Pro Leu Gln Asp Asn Thr Gly 
                325                 330                 335     


Glu Val Val Ala Val Lys Lys Leu Gln His Ser Thr Glu Glu His Leu 
            340                 345                 350         


Arg Asp Phe Glu Arg Glu Ile Glu Ile Leu Lys Ser Leu Gln His Asp 
        355                 360                 365             


Asn Ile Val Lys Tyr Lys Gly Val Cys Tyr Ser Ala Gly Arg Arg Asn 
    370                 375                 380                 


Leu Lys Leu Ile Met Glu Tyr Leu Pro Tyr Gly Ser Leu Arg Asp Tyr 
385                 390                 395                 400 


Leu Gln Lys His Lys Glu Arg Ile Asp His Ile Lys Leu Leu Gln Tyr 
                405                 410                 415     


Thr Ser Gln Ile Cys Lys Gly Met Glu Tyr Leu Gly Thr Lys Arg Tyr 
            420                 425                 430         


Ile His Arg Asp Leu Ala Thr Arg Asn Ile Leu Val Glu Asn Glu Asn 
        435                 440                 445             


Arg Val Lys Ile Gly Asp Phe Gly Leu Thr Lys Val Leu Pro Gln Asp 
    450                 455                 460                 


Lys Glu Tyr Tyr Lys Val Lys Glu Pro Gly Glu Ser Pro Ile Phe Trp 
465                 470                 475                 480 


Tyr Ala Pro Glu Ser Leu Thr Glu Ser Lys Phe Ser Val Ala Ser Asp 
                485                 490                 495     


Val Trp Ser Phe Gly Val Val Leu Tyr Glu Leu Phe Thr Tyr Ile Glu 
            500                 505                 510         


Lys Ser Lys Ser Pro Pro Ala Glu Phe Met Arg Met Ile Gly Asn Asp 
        515                 520                 525             


Lys Gln Gly Gln Met Ile Val Phe His Leu Ile Glu Leu Leu Lys Asn 
    530                 535                 540                 


Asn Gly Arg Leu Pro Arg Pro Asp Gly Cys Pro Asp Glu Ile Tyr Met 
545                 550                 555                 560 


Ile Met Thr Glu Cys Trp Asn Asn Asn Val Asn Gln Arg Pro Ser Phe 
                565                 570                 575     


Arg Asp Leu Ala Leu Arg Val Asp Gln Ile Arg Asp Asn Met 
            580                 585                 590 


<210>  51
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  51

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  52
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TeV protease domain

<400>  52

Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile 
1               5                   10                  15      


Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly 
            20                  25                  30          


Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg 
        35                  40                  45              


Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val 
    50                  55                  60                  


Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met 
65                  70                  75                  80  


Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu 
                85                  90                  95      


Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr 
            100                 105                 110         


Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys 
        115                 120                 125             


Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr 
    130                 135                 140                 


Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe 
145                 150                 155                 160 


Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr 
                165                 170                 175     


Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu 
            180                 185                 190         


Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu 
        195                 200                 205             


Trp Gly Gly His Lys Val Phe Met Ser Lys Pro Glu Glu Pro Phe Gln 
    210                 215                 220                 


Pro Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr Ser Gln 
225                 230                 235                 240 


<210>  53
<211>  541
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70-SH2 domain fused to a TEV protease sequence

<400>  53

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ser Gly Gly Gly Gly Ser Gly Gly 
        275                 280                 285             


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser Leu Phe 
    290                 295                 300                 


Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu 
305                 310                 315                 320 


Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe 
                325                 330                 335     


Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly 
            340                 345                 350         


Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asn Thr 
        355                 360                 365             


Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile 
    370                 375                 380                 


Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg 
385                 390                 395                 400 


Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln 
                405                 410                 415     


Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro 
            420                 425                 430         


Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly 
        435                 440                 445             


Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly 
    450                 455                 460                 


Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser 
465                 470                 475                 480 


Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln 
                485                 490                 495     


Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly 
            500                 505                 510         


His Lys Val Phe Met Ser Lys Pro Glu Glu Pro Phe Gln Pro Val Lys 
        515                 520                 525             


Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr Ser Gln 
    530                 535                 540     


<210>  54
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PTPN6-SH2 domain fused to a TEV protease sequence

<400>  54

Met Val Arg Trp Phe His Arg Asp Leu Ser Gly Leu Asp Ala Glu Thr 
1               5                   10                  15      


Leu Leu Lys Gly Arg Gly Val His Gly Ser Phe Leu Ala Arg Pro Ser 
            20                  25                  30          


Arg Lys Asn Gln Gly Asp Phe Ser Leu Ser Val Arg Val Gly Asp Gln 
        35                  40                  45              


Val Thr His Ile Arg Ile Gln Asn Ser Gly Asp Phe Tyr Asp Leu Tyr 
    50                  55                  60                  


Gly Gly Glu Lys Phe Ala Thr Leu Thr Glu Leu Val Glu Tyr Tyr Thr 
65                  70                  75                  80  


Gln Gln Gln Gly Val Leu Gln Asp Arg Asp Gly Thr Ile Ile His Leu 
                85                  90                  95      


Lys Tyr Pro Leu Asn Cys Ser Asp Pro Thr Ser Glu Arg Trp Tyr His 
            100                 105                 110         


Gly His Met Ser Gly Gly Gln Ala Glu Thr Leu Leu Gln Ala Lys Gly 
        115                 120                 125             


Glu Pro Trp Thr Phe Leu Val Arg Glu Ser Leu Ser Gln Pro Gly Asp 
    130                 135                 140                 


Phe Val Leu Ser Val Leu Ser Asp Gln Pro Lys Ala Gly Pro Gly Ser 
145                 150                 155                 160 


Pro Leu Arg Val Thr His Ile Lys Val Met Cys Glu Gly Gly Arg Tyr 
                165                 170                 175     


Thr Val Gly Gly Leu Glu Thr Phe Asp Ser Leu Thr Asp Leu Val Glu 
            180                 185                 190         


His Phe Lys Lys Thr Gly Ile Glu Glu Ala Ser Gly Ala Phe Val Tyr 
        195                 200                 205             


Leu Arg Gln Pro Tyr Tyr Ser Gly Gly Gly Gly Ser Ser Leu Phe Lys 
    210                 215                 220                 


Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His Leu Thr 
225                 230                 235                 240 


Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly Phe Gly 
                245                 250                 255     


Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn Gly Thr 
            260                 265                 270         


Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asn Thr Thr 
        275                 280                 285             


Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile Ile Arg 
    290                 295                 300                 


Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe Arg Glu 
305                 310                 315                 320 


Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe Gln Thr 
                325                 330                 335     


Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe Pro Ser 
            340                 345                 350         


Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp Gly Gln 
        355                 360                 365             


Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val Gly Ile 
    370                 375                 380                 


His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr Ser Val 
385                 390                 395                 400 


Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln Gln Trp 
                405                 410                 415     


Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly Gly His 
            420                 425                 430         


Lys Val Phe Met Ser Lys Pro Glu Glu Pro Phe Gln Pro Val Lys Glu 
        435                 440                 445             


Ala Thr Gln Leu Met Asn Glu Leu Val Tyr Ser Gln 
    450                 455                 460 


<210>  55
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  membrane tethered transcription factor

<400>  55

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly 
            20                  25                  30          


Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser 
        35                  40                  45              


Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser 
    50                  55                  60                  


Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro 
65                  70                  75                  80  


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                85                  90                  95      


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            100                 105                 110         


Asp Phe Ala Cys Asp Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly 
        115                 120                 125             


Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Cys Val Arg Cys Arg 
    130                 135                 140                 


His Arg Arg Arg Gln Ala Glu Arg Met Ala Gln Ile Lys Arg Val Val 
145                 150                 155                 160 


Ser Glu Lys Lys Thr Ala Gln Ala Pro His Arg Phe Gln Lys Thr Cys 
                165                 170                 175     


Ser Pro Ile Ser Gly Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Met 
            180                 185                 190         


Pro Lys Lys Lys Arg Lys Val Ala Pro Pro Thr Asp Val Ser Leu Gly 
        195                 200                 205             


Asp Glu Leu His Leu Asp Gly Glu Asp Val Ala Met Ala His Ala Asp 
    210                 215                 220                 


Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Asp Gly Asp Ser Pro 
225                 230                 235                 240 


Gly Pro Gly Phe Thr Pro His Asp Ser Ala Pro Tyr Gly Ala Leu Asp 
                245                 250                 255     


Met Ala Asp Phe Glu Phe Glu Gln Met Phe Thr Asp Ala Leu Gly Ile 
            260                 265                 270         


Asp Glu Tyr Gly Gly Ser Gly Gly Gly Ser Met Gln Ile Leu Val Ala 
        275                 280                 285             


Ser Asp Ala Thr Met Lys Leu Leu Ser Ser Ile Glu Gln Ala Cys Asp 
    290                 295                 300                 


Ile Cys Arg Leu Lys Lys Leu Lys Cys Ser Lys Glu Lys Pro Lys Cys 
305                 310                 315                 320 


Ala Lys Cys Leu Lys Asn Asn Trp Glu Cys Arg Tyr Ser Pro Lys Thr 
                325                 330                 335     


Lys Arg Ser Pro Leu Thr Arg Ala His Leu Thr Glu Val Glu Ser Arg 
            340                 345                 350         


Leu Glu Arg Leu Glu Gln Leu Phe Leu Leu Ile Phe Pro Arg Glu Asp 
        355                 360                 365             


Leu Asp Met Ile Leu Lys Met Asp Ser Leu Gln Asp Ile Lys Ala Leu 
    370                 375                 380                 


Leu Thr Gly Leu Phe Val Gln Asp Asn Val Asn Lys Asp Ala Val Thr 
385                 390                 395                 400 


Asp Arg Leu Ala Ser Val Glu Thr Asp Met Pro Leu Thr Leu Arg Gln 
                405                 410                 415     


His Arg Ile Ser Ala Thr Ser Ser Ser Glu Glu Ser Ser Asn Lys Gly 
            420                 425                 430         


Gln Arg Gln Leu Thr Val 
        435             


<210>  56
<211>  438
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  target receptor

<400>  56

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Asp 
        35                  40                  45              


Ile Tyr Phe Asn Leu Val Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Asp Thr Asn Arg Leu Ala Asp Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln His Tyr Lys 
            100                 105                 110         


Asn Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Arg Ser Glu Val Gln Leu Val Glu Ser Gly Gly 
145                 150                 155                 160 


Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                165                 170                 175     


Gly Phe Thr Leu Ser Asn Tyr Gly Met His Trp Ile Arg Gln Ala Pro 
            180                 185                 190         


Gly Lys Gly Leu Glu Trp Val Ser Ser Ile Ser Leu Asn Gly Gly Ser 
        195                 200                 205             


Thr Tyr Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
    210                 215                 220                 


Asn Ala Lys Ser Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu 
225                 230                 235                 240 


Asp Thr Ala Val Tyr Tyr Cys Ala Ala Gln Asp Ala Tyr Thr Gly Gly 
                245                 250                 255     


Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Met 
            260                 265                 270         


Asp Pro Ala Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val 
        275                 280                 285             


His Pro Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro 
    290                 295                 300                 


Arg Gly Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
305                 310                 315                 320 


Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp 
                325                 330                 335     


Val Leu Ala Val Ile Cys Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala 
            340                 345                 350         


Arg Arg Thr Gly Gln Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val 
        355                 360                 365             


Phe Ser Val Asp Tyr Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr 
    370                 375                 380                 


Pro Glu Pro Pro Val Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr 
385                 390                 395                 400 


Ile Val Phe Pro Ser Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly 
                405                 410                 415     


Ser Ala Asp Gly Pro Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly 
            420                 425                 430         


His Cys Ser Trp Pro Leu 
        435             


<210>  57
<211>  509
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  receptor containing a CAR against CD19 with a cleavable CD3-zeta 
       endodomain

<400>  57

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Cys Arg His Arg Arg Arg 
            340                 345                 350         


Gln Ala Glu Arg Met Ala Gln Ile Lys Arg Val Val Ser Glu Lys Lys 
        355                 360                 365             


Thr Ala Gln Ala Pro His Arg Phe Gln Lys Thr Cys Ser Pro Ile Ser 
    370                 375                 380                 


Gly Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Met Arg Arg Val Lys 
385                 390                 395                 400 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
                405                 410                 415     


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            420                 425                 430         


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        435                 440                 445             


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    450                 455                 460                 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
465                 470                 475                 480 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                485                 490                 495     


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505                 


<210>  58
<211>  827
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  receptor containing a CAR against CD19 with a CD3-zeta endodomain
       and a cleavable CD148 endodomain

<400>  58

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Lys Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
    130                 135                 140                 


Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu Ser Gly Pro Gly 
145                 150                 155                 160 


Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys Thr Val Ser Gly 
                165                 170                 175     


Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg Gln Pro Pro Arg 
            180                 185                 190         


Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser Glu Thr Thr Tyr 
        195                 200                 205             


Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile Lys Asp Asn Ser 
    210                 215                 220                 


Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 
225                 230                 235                 240 


Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly Gly Ser Tyr Ala 
                245                 250                 255     


Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Phe Trp 
305                 310                 315                 320 


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                325                 330                 335     


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg 
            340                 345                 350         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        355                 360                 365             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    370                 375                 380                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
385                 390                 395                 400 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                405                 410                 415     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            420                 425                 430         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        435                 440                 445             


Ala Leu His Met Gln Ala Leu Pro Pro Arg Glu Asn Leu Tyr Phe Gln 
    450                 455                 460                 


Met Ala Val Phe Gly Cys Ile Phe Gly Ala Leu Val Ile Val Thr Val 
465                 470                 475                 480 


Gly Gly Phe Ile Phe Trp Arg Lys Lys Arg Lys Asp Ala Lys Asn Asn 
                485                 490                 495     


Glu Val Ser Phe Ser Gln Ile Lys Pro Lys Lys Ser Lys Leu Ile Arg 
            500                 505                 510         


Val Glu Asn Phe Glu Ala Tyr Phe Lys Lys Gln Gln Ala Asp Ser Asn 
        515                 520                 525             


Cys Gly Phe Ala Glu Glu Tyr Glu Asp Leu Lys Leu Val Gly Ile Ser 
    530                 535                 540                 


Gln Pro Lys Tyr Ala Ala Glu Leu Ala Glu Asn Arg Gly Lys Asn Arg 
545                 550                 555                 560 


Tyr Asn Asn Val Leu Pro Tyr Asp Ile Ser Arg Val Lys Leu Ser Val 
                565                 570                 575     


Gln Thr His Ser Thr Asp Asp Tyr Ile Asn Ala Asn Tyr Met Pro Gly 
            580                 585                 590         


Tyr His Ser Lys Lys Asp Phe Ile Ala Thr Gln Gly Pro Leu Pro Asn 
        595                 600                 605             


Thr Leu Lys Asp Phe Trp Arg Met Val Trp Glu Lys Asn Val Tyr Ala 
    610                 615                 620                 


Ile Ile Met Leu Thr Lys Cys Val Glu Gln Gly Arg Thr Lys Cys Glu 
625                 630                 635                 640 


Glu Tyr Trp Pro Ser Lys Gln Ala Gln Asp Tyr Gly Asp Ile Thr Val 
                645                 650                 655     


Ala Met Thr Ser Glu Ile Val Leu Pro Glu Trp Thr Ile Arg Asp Phe 
            660                 665                 670         


Thr Val Lys Asn Ile Gln Thr Ser Glu Ser His Pro Leu Arg Gln Phe 
        675                 680                 685             


His Phe Thr Ser Trp Pro Asp His Gly Val Pro Asp Thr Thr Asp Leu 
    690                 695                 700                 


Leu Ile Asn Phe Arg Tyr Leu Val Arg Asp Tyr Met Lys Gln Ser Pro 
705                 710                 715                 720 


Pro Glu Ser Pro Ile Leu Val His Cys Ser Ala Gly Val Gly Arg Thr 
                725                 730                 735     


Gly Thr Phe Ile Ala Ile Asp Arg Leu Ile Tyr Gln Ile Glu Asn Glu 
            740                 745                 750         


Asn Thr Val Asp Val Tyr Gly Ile Val Tyr Asp Leu Arg Met His Arg 
        755                 760                 765             


Pro Leu Met Val Gln Thr Glu Asp Gln Tyr Val Phe Leu Asn Gln Cys 
    770                 775                 780                 


Val Leu Asp Ile Val Arg Ser Gln Lys Asp Ser Lys Val Asp Leu Ile 
785                 790                 795                 800 


Tyr Gln Asn Thr Thr Ala Met Thr Ile Tyr Glu Asn Leu Ala Pro Val 
                805                 810                 815     


Thr Thr Phe Gly Lys Thr Asn Gly Tyr Ile Ala 
            820                 825         


<210>  59
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2a self-cleaving peptide

<400>  59

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  60
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2a self-cleaving peptide

<400>  60

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  61
<211>  563
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dual SH2 domains from SHP-2 fused to ZAP70 kinase domain

<400>  61

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu Asn Cys Ala Asp Pro Thr Ser Glu Arg Trp Phe His Gly His Leu 
            100                 105                 110         


Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr Glu Lys Gly Lys His Gly 
        115                 120                 125             


Ser Phe Leu Val Arg Glu Ser Gln Ser His Pro Gly Asp Phe Val Leu 
    130                 135                 140                 


Ser Val Arg Thr Gly Asp Asp Lys Gly Glu Ser Asn Asp Gly Lys Ser 
145                 150                 155                 160 


Lys Val Thr His Val Met Ile Arg Cys Gln Glu Leu Lys Tyr Asp Val 
                165                 170                 175     


Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr Asp Leu Val Glu His Tyr 
            180                 185                 190         


Lys Lys Asn Pro Met Val Glu Thr Leu Gly Thr Val Leu Gln Leu Lys 
        195                 200                 205             


Gln Pro Leu Asn Thr Thr Arg Ile Asn Pro Asn Ser Ser Ala Ser Asn 
    210                 215                 220                 


Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala His Pro Ser Thr Leu 
225                 230                 235                 240 


Thr His Pro Gln Arg Arg Ile Asp Thr Leu Asn Ser Asp Gly Tyr Thr 
                245                 250                 255     


Pro Glu Pro Ala Arg Ile Thr Ser Pro Asp Lys Pro Arg Pro Met Pro 
            260                 265                 270         


Met Asp Thr Ser Val Tyr Glu Ser Pro Tyr Ser Asp Pro Glu Glu Leu 
        275                 280                 285             


Lys Asp Lys Lys Leu Phe Leu Lys Arg Asp Asn Leu Leu Ile Ala Asp 
    290                 295                 300                 


Ile Glu Leu Gly Cys Gly Asn Phe Gly Ser Val Arg Gln Gly Val Tyr 
305                 310                 315                 320 


Arg Met Arg Lys Lys Gln Ile Asp Val Ala Ile Lys Val Leu Lys Gln 
                325                 330                 335     


Gly Thr Glu Lys Ala Asp Thr Glu Glu Met Met Arg Glu Ala Gln Ile 
            340                 345                 350         


Met His Gln Leu Asp Asn Pro Tyr Ile Val Arg Leu Ile Gly Val Cys 
        355                 360                 365             


Gln Ala Glu Ala Leu Met Leu Val Met Glu Met Ala Gly Gly Gly Pro 
    370                 375                 380                 


Leu His Lys Phe Leu Val Gly Lys Arg Glu Glu Ile Pro Val Ser Asn 
385                 390                 395                 400 


Val Ala Glu Leu Leu His Gln Val Ser Met Gly Met Lys Tyr Leu Glu 
                405                 410                 415     


Glu Lys Asn Phe Val His Arg Asp Leu Ala Ala Arg Asn Val Leu Leu 
            420                 425                 430         


Val Asn Arg His Tyr Ala Lys Ile Ser Asp Phe Gly Leu Ser Lys Ala 
        435                 440                 445             


Leu Gly Ala Asp Asp Ser Tyr Tyr Thr Ala Arg Ser Ala Gly Lys Trp 
    450                 455                 460                 


Pro Leu Lys Trp Tyr Ala Pro Glu Cys Ile Asn Phe Arg Lys Phe Ser 
465                 470                 475                 480 


Ser Arg Ser Asp Val Trp Ser Tyr Gly Val Thr Met Trp Glu Ala Leu 
                485                 490                 495     


Ser Tyr Gly Gln Lys Pro Tyr Lys Lys Met Lys Gly Pro Glu Val Met 
            500                 505                 510         


Ala Phe Ile Glu Gln Gly Lys Arg Met Glu Cys Pro Pro Glu Cys Pro 
        515                 520                 525             


Pro Glu Leu Tyr Ala Leu Met Ser Asp Cys Trp Ile Tyr Lys Trp Glu 
    530                 535                 540                 


Asp Arg Pro Asp Phe Leu Thr Val Glu Gln Arg Met Arg Ala Cys Tyr 
545                 550                 555                 560 


Tyr Ser Leu 
            


<210>  62
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  dual SHP-2 SH2 domain fused to an Akt kinase domain

<400>  62

Trp Phe His Pro Asn Ile Thr Gly Val Glu Ala Glu Asn Leu Leu Leu 
1               5                   10                  15      


Thr Arg Gly Val Asp Gly Ser Phe Leu Ala Arg Pro Ser Lys Ser Asn 
            20                  25                  30          


Pro Gly Asp Phe Thr Leu Ser Val Arg Arg Asn Gly Ala Val Thr His 
        35                  40                  45              


Ile Lys Ile Gln Asn Thr Gly Asp Tyr Tyr Asp Leu Tyr Gly Gly Glu 
    50                  55                  60                  


Lys Phe Ala Thr Leu Ala Glu Leu Val Gln Tyr Tyr Met Glu His His 
65                  70                  75                  80  


Gly Gln Leu Lys Glu Lys Asn Gly Asp Val Ile Glu Leu Lys Tyr Pro 
                85                  90                  95      


Leu Asn Cys Ala Asp Pro Thr Ser Glu Arg Trp Phe His Gly His Leu 
            100                 105                 110         


Ser Gly Lys Glu Ala Glu Lys Leu Leu Thr Glu Lys Gly Lys His Gly 
        115                 120                 125             


Ser Phe Leu Val Arg Glu Ser Gln Ser His Pro Gly Asp Phe Val Leu 
    130                 135                 140                 


Ser Val Arg Thr Gly Asp Asp Lys Gly Glu Ser Asn Asp Gly Lys Ser 
145                 150                 155                 160 


Lys Val Thr His Val Met Ile Arg Cys Gln Glu Leu Lys Tyr Asp Val 
                165                 170                 175     


Gly Gly Gly Glu Arg Phe Asp Ser Leu Thr Asp Leu Val Glu His Tyr 
            180                 185                 190         


Lys Lys Asn Pro Met Val Glu Thr Leu Gly Thr Val Leu Gln Leu Lys 
        195                 200                 205             


Gln Pro Leu Asn Thr Thr Arg Ile Asn Ala Glu Glu Met Glu Val Ser 
    210                 215                 220                 


Leu Ala Lys Pro Lys His Arg Val Thr Met Asn Glu Phe Glu Tyr Leu 
225                 230                 235                 240 


Lys Leu Leu Gly Lys Gly Thr Phe Gly Lys Val Ile Leu Val Lys Glu 
                245                 250                 255     


Lys Ala Thr Gly Arg Tyr Tyr Ala Met Lys Ile Leu Lys Lys Glu Val 
            260                 265                 270         


Ile Val Ala Lys Asp Glu Val Ala His Thr Leu Thr Glu Asn Arg Val 
        275                 280                 285             


Leu Gln Asn Ser Arg His Pro Phe Leu Thr Ala Leu Lys Tyr Ser Phe 
    290                 295                 300                 


Gln Thr His Asp Arg Leu Cys Phe Val Met Glu Tyr Ala Asn Gly Gly 
305                 310                 315                 320 


Glu Leu Phe Phe His Leu Ser Arg Glu Arg Val Phe Ser Glu Asp Arg 
                325                 330                 335     


Ala Arg Phe Tyr Gly Ala Glu Ile Val Ser Ala Leu Asp Tyr Leu His 
            340                 345                 350         


Ser Glu Lys Asn Val Val Tyr Arg Asp Leu Lys Leu Glu Asn Leu Met 
        355                 360                 365             


Leu Asp Lys Asp Gly His Ile Lys Ile Thr Asp Phe Gly Leu Cys Lys 
    370                 375                 380                 


Glu Gly Ile Lys Asp Gly Ala Thr Met Lys Thr Phe Cys Gly Thr Pro 
385                 390                 395                 400 


Glu Tyr Leu Ala Pro Glu Val Leu Glu Asp Asn Asp Tyr Gly Arg Ala 
                405                 410                 415     


Val Asp Trp Trp Gly Leu Gly Val Val Met Tyr Glu Met Met Cys Gly 
            420                 425                 430         


Arg Leu Pro Phe Tyr Asn Gln Asp His Glu Lys Leu Phe Glu Leu Ile 
        435                 440                 445             


Leu Met Glu Glu Ile Arg Phe Pro Arg Thr Leu Gly Pro Glu Ala Lys 
    450                 455                 460                 


Ser Leu Leu Ser Gly Leu Leu Lys Lys Asp Pro Lys Gln Arg Leu Gly 
465                 470                 475                 480 


Gly Gly Ser Glu Asp Ala Lys Glu Ile Met Gln His Arg Phe Phe Ala 
                485                 490                 495     


Gly Ile Val Trp Gln His Val Tyr Glu Lys Lys Leu Ser Pro Pro Phe 
            500                 505                 510         


Lys Pro Gln Val Thr Ser Glu Thr Asp Thr Arg Tyr Phe Asp Glu Glu 
        515                 520                 525             


Phe Thr Ala Gln Met Ile Thr Ile Thr Pro Pro Asp Gln Asp Asp Ser 
    530                 535                 540                 


Met Glu Cys Val Asp Ser Glu Arg Arg Pro His Phe Pro Gln Phe Ser 
545                 550                 555                 560 


Tyr Ser Ala Ser Gly Thr Ala 
                565         


<210>  63
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITIM conserved sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Ser, Ile, Val or Leu

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa may be Ile, Val or Leu

<400>  63

Xaa Xaa Tyr Xaa Xaa Xaa 
1               5       


