
                                SEQUENCE LISTING

<110> Tector, A Joseph

<120> Transgenic Pigs that Reduce Transplant
  Rejection

<130> HLA_SLA_Prov

<160> 6

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> gRNA A SLA I target

<400> 1
ccaggaccag agccaggaca tgg                                         23

<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> gRNA B SLA I target

<400> 2
gagaccaggc cctcagggga tgg                                         23

<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> gRNA C SLA target

<400> 3
ccagaagtgg gcggccctgg tgg                                         23

<210> 4
<211> 5821
<212> DNA
<213> Artificial Sequence

<220> 
<223> HLA A2 vector

<220> 
<221> misc_feature   
<222> (613)...(667)
<223> SLA 5' homology arm

<220> 
<221> misc_feature   
<222> (685)...(768)
<223> PHi Recombinase ATTP site

<220> 
<221> misc_feature   
<222> (780)...(1806)
<223> hygromycin resistance gene

<220> 
<221> misc_feature   
<222> (1821)...(2047)
<223> SV40 poly A tail


<220> 
<221> misc_feature   
<222> (2079)...(2163)
<223> Phi Recombinase ATTB site


<220> 
<221> misc_feature   
<222> (2175)...(3273)
<223> HLA-A2 ORF


<220> 
<221> misc_feature   
<222> (3274)...(3501)
<223> BGH polyA


<220> 
<221> misc_feature   
<222> (3515)...(3734)
<223> SLA-3' homology arm

<400> 4
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcggcctgc gcaacacgcg atcgcggcgc gccaccatgg accatggtaa 360
tgatagcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 420
ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 480
cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 540
gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggagccac 600
catggaatcg atataagctc tccacccacc cggctctgct cagcttctcc ccagaccccg 660
aggctgagga tcatcgatct taagagaagc ggttttcggg agtagtgccc caactggggt 720
aacctttgag ttctctcagt tgggggcgta gggtcgccga catgacacct taaggatatc 780
atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac 840
agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat 900
gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat 960
cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt 1020
ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg 1080
caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat 1140
gccatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga 1200
atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat 1260
cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag 1320
ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc 1380
tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg 1440
atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct 1500
tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg 1560
cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac 1620
ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga 1680
gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc 1740
tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag 1800
gaatgaacct gagctagctg gccagacatg ataagataca ttgatgagtt tggacaaacc 1860
acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 1920
tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 1980
tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 2040
ggtatgggtc gacgatatcg tttaaacacg cgttctagat cacggtctcg aagccgcggt 2100
gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt 2160
ccatctagat tcgaaatggc cgtcatggcg ccccgaaccc tcgtcctgct actctcgggg 2220
gctctggccc tgacccagac ctgggcgggc tctcactcca tgaggtattt cttcacatcc 2280
gtgtcccggc ccggccgcgg ggagccccgc ttcatcgcag tgggctacgt ggacgacacg 2340
cagttcgtgc ggttcgacag cgacgccgcg agccagagga tggagccgcg ggcgccgtgg 2400
atagagcagg agggtccgga gtattgggac ggggagacac ggaaagtgaa ggcccactca 2460
cagactcacc gagtggacct ggggaccctg cgcggctact acaaccagag cgaggccggt 2520
tctcacaccg tccagaggat gtatggctgc gacgtggggt cggactggcg cttcctccgc 2580
gggtaccacc agtacgccta cgacggcaag gattacatcg ccctgaaaga ggacctgcgc 2640
tcttggaccg cggcggacat ggcagctcag accaccaagc acaagtggga ggcggcccat 2700
gtggcggagc agttgagagc ctacctggag ggcacgtgcg tggagtggct ccgcagatac 2760
ctggagaacg ggaaggagac gctgcagcgc acggacgccc ccaaaacgca tatgactcac 2820
cacgctgtct ctgaccatga agccaccctg aggtgctggg ccctgagctt ctaccctgcg 2880
gagatcacac tgacctggca gcgggatggg gaggaccaga cccaggacac ggagctcgtg 2940
gagaccaggc ctgcagggga tggaaccttc cagaagtggg cggctgtggt ggtgccttct 3000
ggacaggagc agagatacac ctgccatgtg cagcatgagg gtttgcccaa gcccctcacc 3060
ctgagatggg agccgtcttc ccagcccacc atccccatcg tgggcatcat tgctggcctg 3120
gttctctttg gagctgtgat cactggagct gtggtcgctg ctgtgatgtg gaggaggaag 3180
agctcagata gaaaaggagg gagctactct caggctgcaa gcagtgacag tgcccagggc 3240
tctgatgtgt ctctcacagc ttgtaaagtg tgacgactgt gccttctagt tgccagccat 3300
ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 3360
tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 3420
ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg 3480
gggatgcggt gggctctatg gttcgaatta attaaggact gagggacaca agatttgttc 3540
acatcccact ttgtgacttc agatcccctg actcctattt ctgcagctgc ctctgaaagt 3600
gtctgtgttc ctatgagcat cctgagagga ggtggggacc ctggcccagc cctgcccccc 3660
acgtcccctc ctcaccctga cctgtgttct cttccctgat cctctttcca gttcttgcaa 3720
gtgggagctg ggctttaatt aatgcgcagc gatcgcggcc gccagctgca ttaatgaatc 3780
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 3840
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 3900
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 3960
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 4020
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 4080
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 4140
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 4200
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 4260
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 4320
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 4380
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 4440
agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 4500
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 4560
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 4620
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 4680
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 4740
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 4800
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 4860
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 4920
ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 4980
actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 5040
ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 5100
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 5160
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 5220
ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 5280
ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 5340
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 5400
agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 5460
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 5520
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 5580
aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 5640
tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 5700
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 5760
gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 5820
c                                                                 5821



<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> gRNA 5' SLA

<400> 5
ccccgaggct gaggatcatg ggg                                         23

<210> 6
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> gRNA 3' SLA

<400> 6
aactgccttg tggggactga ggg                                         23
