                         SEQUENCE LISTING

<110>  CureVac GmbH
 
<120>  Method for increasing replication of a circular DNA molecule

<130>  C 9828WO2/MH

<150>  PCT/EP2015/068398
<151>  2015-08-10

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  poly(A) and poly(c) sequence

<400>  1
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaatgcatc cccccccccc cccccccccc cccccccccc                            100


<210>  2
<211>  2083
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  GC-optimized mRNA H1N1

<400>  2
ggggcgcugc cuacggaggu ggcagccauc uccuucucgg caucaagcuu accaugaagg       60

ccauccuggu gguccuccug uacaccuucg ccaccgcgaa cgccgacacg cugugcaucg      120

gcuaccacgc caacaacagc accgacaccg uggacaccgu gcucgagaag aacgucacgg      180

ugacccacuc cgugaaccug cuggaggaca agcacaacgg gaagcucugc aagcugcggg      240

gcgucgcccc gcugcaccuc gggaagugca acaucgccgg cuggauccug gggaacccgg      300

agugcgagag ccuguccacc gcgagcuccu ggagcuacau cguggagacc uccagcuccg      360

acaacggcac gugcuacccc ggcgacuuca ucgacuacga ggagcuccgc gagcagcuga      420

gcuccgugag cuccuucgag cgguucgaga ucuuccccaa gaccagcucc uggcccaacc      480

acgacagcaa caaggggguc accgccgccu gcccgcacgc cggcgcgaag uccuucuaca      540

agaaccugau cuggcucgug aagaagggga acagcuaccc caagcugucc aagagcuaca      600

ucaacgacaa gggcaaggag gugcuggucc ucugggggau ccaccacccc agcaccuccg      660

ccgaccagca gagccuguac cagaacgccg acgccuacgu guucgugggc uccagccgcu      720

acuccaagaa guucaagccc gagaucgcca uccggccgaa gguccgcgac caggagggcc      780

ggaugaacua cuacuggacg cugguggagc ccggggacaa gaucaccuuc gaggcgaccg      840

gcaaccucgu ggucccccgc uacgccuucg ccauggagcg gaacgccggg agcggcauca      900

ucaucuccga cacccccgug cacgacugca acacgaccug ccagaccccg aagggcgcca      960

ucaacaccag ccugcccuuc cagaacaucc accccaucac gaucgggaag ugccccaagu     1020

acgugaaguc caccaagcug cgccucgcga ccggccugcg gaacgucccg agcauccagu     1080

cccgcgggcu guucggcgcc aucgccgggu ucaucgaggg cggcuggacc gggauggugg     1140

acggcuggua cggguaccac caccagaacg agcagggcag cggguacgcc gccgaccuca     1200

aguccacgca gaacgcgauc gacgagauca ccaacaaggu gaacagcguc aucgagaaga     1260

ugaacaccca guucaccgcc gugggcaagg aguucaacca ccuggagaag cggaucgaga     1320

accugaacaa gaaggucgac gacggcuucc ucgacaucug gacguacaac gccgagcugc     1380

uggugcuccu ggagaacgag cgcacccugg acuaccacga cuccaacgug aagaaccucu     1440

acgagaaggu ccggagccag cugaagaaca acgccaagga gaucgggaac ggcugcuucg     1500

aguucuacca caagugcgac aacaccugca uggaguccgu gaagaacggg accuacgacu     1560

accccaagua cagcgaggag gccaagcuga accgcgagga gaucgacggc gugaagcucg     1620

aguccacgcg gaucuaccag auccuggcga ucuacagcac cgucgccagc ucccuggugc     1680

ucguggucag ccugggggcc aucuccuucu ggaugugcag caacggcucc cugcagugcc     1740

gcaucugcau cugaccacua gugcaucaca uuuaaaagca ucucagccua ccaugagaau     1800

aagagaaaga aaaugaagau caauagcuua uucaucucuu uuucuuuuuc guugguguaa     1860

agccaacacc cugucuaaaa aacauaaauu ucuuuaauca uuuugccucu uuucucugug     1920

cuucaauuaa uaaaaaaugg aaagaaccua gaucuaaaaa aaaaaaaaaa aaaaaaaaaa     1980

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaau gcaucccccc cccccccccc     2040

cccccccccc cccccaaagg cucuuuucag agccaccaga auu                       2083


<210>  3
<211>  285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAS-BH

<400>  3
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac       60

ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc      120

gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta      180

tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt      240

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgc                      285


<210>  4
<211>  1520
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1140-AF-backbone

<400>  4
ggtacctaat gatttttatc aaaatcatta agttaaggta gatacacatc ttgtcatatg       60

atcaaatggt ttcgccaaaa atcaataatc agacaacaag atgtgcgaac tcgatatttt      120

acacgactct ctttaccaat tctaccacaa cgtggtttgc aggagtcagg caactatgga      180

tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc      240

agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag      300

gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc      360

gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt      420

tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt      480

gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat      540

accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc      600

accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa      660

gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg      720

ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag      780

atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag      840

gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa      900

cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt      960

gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg     1020

gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc     1080

tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac     1140

cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct     1200

tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga     1260

tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg     1320

ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc     1380

gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca     1440

tcaccgaaac gcgcgaggca gcctaagaaa aaaaagcccg ctcattaggc gggctagcga     1500

cgtctaatac gactcactat                                                 1520


<210>  5
<211>  2284
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1140-K-backbone

<400>  5
gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc       60

atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag      120

gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat      180

caatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga      240

atccggtgag aatggcaaaa gtttgtgcat ttctttccag acttgttcaa caggccagcc      300

attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc      360

ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg      420

taaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc      480

ttctaatacc tggaaggctg ttttcccagg gatcgcagtg gtgagtaacc aagcatcatc      540

aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag      600

tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa      660

ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt      720

atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct      780

agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta      840

agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag      900

attttgagac acaacgtggt ttgcaggagt caggcaacta tggatgaacg aaatagacag      960

atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca     1020

tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc     1080

ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca     1140

gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     1200

tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     1260

ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt     1320

ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     1380

gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     1440

ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     1500

tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     1560

ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     1620

agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     1680

agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     1740

gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     1800

tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt     1860

accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca     1920

gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt     1980

atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc     2040

cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa     2100

cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg     2160

tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga     2220

ggcagcctaa gaaaaaaaag cccgctcatt aggcgggcta gcgacgtcta atacgactca     2280

ctat                                                                  2284


<210>  6
<211>  1015
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC origin

<400>  6
ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga       60

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac      120

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa      180

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat      240

cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc      300

taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg      360

gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc      420

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg      480

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg      540

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa      600

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg      660

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga      720

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct      780

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca      840

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc      900

ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg      960

ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga          1015


<210>  7
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBR322 origin

<400>  7
ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt       60

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc      120

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa      180

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct      240

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta      300

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg      360

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc      420

ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta      480

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg      540

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctca                  589


<210>  8
<211>  582
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMB1 origin

<400>  8
gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc       60

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga      120

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt      180

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg      240

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc      300

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg      360

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc      420

ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg      480

ctgaagccag ttccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg      540

ctggtagcgg tggttttttt gtttgcaagc agcagattac gc                         582


<210>  9
<211>  602
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ColE1 origin

<400>  9
aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc       60

accgctacca acggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt      120

aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagtcggg      180

ccactacttc aagaactctg tagcaccgtt tgtgccatca tcgctctgct aatccggtta      240

ccagtggctg ctgccagtgg cgttaaggcg tgccttaccg ggttggactc aagacgatag      300

ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg      360

gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg      420

cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag      480

cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc      540

cacctctgac ttgagcgtct atttttgtga tgctcgtcag gggggcggag cctatggaaa      600

aa                                                                     602


<210>  10
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K_gamma origin

<400>  10
aagctttaaa agccttatat attctttttt ttcttataaa acttaaaacc ttagaggcta       60

tttaagttgc tgatttatat taattttatt gttcaaacat gagagcttag tacgtgaaac      120

atgagagctt agtacgttag ccatgagagc ttagtacgtt agccatgagg gtttagttcg      180

ttaaacatga gagcttagta cgttaaacat gagagcttag tacgtgaaac atgagagctt      240

agtacgtact atcaacaggt tgaactgctg atcttcagat ct                         282


<210>  11
<211>  532
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K_beta origin

<400>  11
agatctatgg atgttgagaa tgtagtatgg ttatactgcg atgcaggata gggcaaacgc       60

cgtaaaatga cgtctttgac gttatttttc agcgcttgcc cggtgaccta cagtcggttc      120

ttgttgggag attttatgaa gtttatagta aaggatttta tcagtgataa atatgcaaag      180

gctattaaca ttttaaatga taaccttaaa gaaaactact atgtttttta tggtgtaagg      240

ttaagtgaaa ttctttttcc tgcaagtgat tatggtacag atgatttttt taaggagttt      300

gaggaaataa acaacgttac cttgctttag ttgtttttga aataaatgaa cgtgaacctg      360

tgattgtaat tggttttgat gaaataaatg gtgcgattct tatagagaaa tccggtataa      420

aggttttagt aatcgataat ctatcggcgt tgctgaccaa tgaaacattg ggtgtcttct      480

tcaagtaaca gatctagctg aaaatcgtca tcaaaactgt gcaccttatc tg              532


<210>  12
<211>  639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K alpha origin

<400>  12
tcttacttct ttgcgtagct gttaaataca gcgttgtttt gataaaatca tcattatcat       60

cgataatgct ttcttcaatt tttttatcct tactctttaa taaagcactt gctaataact      120

tcataccttt tgcaactgtc aaatttggtt catcagggta aatgctttta aggcatacta      180

acaaataatc atggtcttca tcttcaactc taaactgaat ttttttcatc ataactccca      240

acaagaaccg actgtaggtc accgggcaaa cgctgaaaaa taacgtcgaa tgacgtcatt      300

ttgcggcgtt tgccctatcc tgcatcgcag tagaaaatgc cacaactgaa attgtgcttc      360

agtatgtaca gaaatgcaaa atctgaggga tttcgtagct gaaagatcgc cagtcttcga      420

ccgtaaggat aggagttgct gtaagacctg tgcggggcgt tcgcttcgcg aacgggtctg      480

gcagggggca caagcgctgt gctgtgatat atgcaaaaga agccacccac gaacgggagg      540

gcttcggcga atcgactata gtgatctatt tacccggctg attgtcgcct tctagccctc      600

gcgggcatca tgcaaccagt gcctgaattt agttatatg                             639


<210>  13
<211>  914
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p15A origin

<400>  13
gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg       60

cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga      120

tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg      180

aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg      240

aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca      300

cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc      360

gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt      420

cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg      480

cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct      540

tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag      600

cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa      660

actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag      720

ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag      780

caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa      840

tatttctagt tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata      900

cgatataagt tgta                                                        914


<210>  14
<211>  300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pSC101 origin

<400>  14
gcggaactga ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt       60

tttattcttt ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca      120

aaggtctagc ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct      180

agcagaattt acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag      240

cccatctcaa ttggtatagt gattaaaatc acctagacca attgagatgt atgtctgaat      300


<210>  15
<211>  307
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F1 phagemid origin

<400>  15
ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc       60

gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt      120

tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag      180

cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg      240

gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc      300

ttaatgc                                                                307


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  histone stem-loop

<400>  16
caaaggctct tttcagagcc acca                                              24


<210>  17
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  histone stem loop

<400>  17
caaaggcucu uuucagagcc acca                                              24


