                               SEQUENCE LISTING

<110> CONRADIE, ALEX
 
<120> METHODS, CELLS AND REAGENTS FOR PRODUCTION OF ISOPRENE, 
      DERIVATIVES AND INTERMEDIATES THEREOF

<130> 12444.0582-00000

<140>
<141>

<150> 62/205,926
<151> 2015-08-17

<160> 14    

<170> PatentIn version 3.5

<210> 1
<211> 425
<212> PRT
<213> Staphylococcus aureus

<400> 1
Met Gln Ser Leu Asp Lys Asn Phe Arg His Leu Ser Arg Gln Gln Lys 
1               5                   10                  15      


Leu Gln Gln Leu Val Asp Lys Gln Trp Leu Ser Glu Asp Gln Phe Asp 
            20                  25                  30          


Ile Leu Leu Asn His Pro Leu Ile Asp Glu Glu Val Ala Asn Ser Leu 
        35                  40                  45              


Ile Glu Asn Val Ile Ala Gln Gly Ala Leu Pro Val Gly Leu Leu Pro 
    50                  55                  60                  


Asn Ile Ile Val Asp Asp Lys Ala Tyr Val Val Pro Met Met Val Glu 
65                  70                  75                  80  


Glu Pro Ser Val Val Ala Ala Ala Ser Tyr Gly Ala Lys Leu Val Asn 
                85                  90                  95      


Gln Thr Gly Gly Phe Lys Thr Val Ser Ser Glu Arg Ile Met Ile Gly 
            100                 105                 110         


Gln Ile Val Phe Asp Gly Val Asp Asp Thr Glu Lys Leu Ser Ala Asp 
        115                 120                 125             


Ile Lys Ala Leu Glu Lys Gln Ile His Lys Ile Ala Asp Glu Ala Tyr 
    130                 135                 140                 


Pro Ser Ile Lys Ala Arg Gly Gly Gly Tyr Gln Arg Ile Ala Ile Asp 
145                 150                 155                 160 


Thr Phe Pro Glu Gln Gln Leu Leu Ser Leu Lys Val Phe Val Asp Thr 
                165                 170                 175     


Lys Asp Ala Met Gly Ala Asn Met Leu Asn Thr Ile Leu Glu Ala Ile 
            180                 185                 190         


Thr Ala Phe Leu Lys Asn Glu Ser Pro Gln Ser Asp Ile Leu Met Ser 
        195                 200                 205             


Ile Leu Ser Asn His Ala Thr Ala Ser Val Val Lys Val Gln Gly Glu 
    210                 215                 220                 


Ile Asp Val Lys Asp Leu Ala Arg Gly Glu Arg Thr Gly Glu Glu Val 
225                 230                 235                 240 


Ala Lys Arg Met Glu Arg Ala Ser Val Leu Ala Gln Val Asp Ile His 
                245                 250                 255     


Arg Ala Ala Thr His Asn Lys Gly Val Met Asn Gly Ile His Ala Val 
            260                 265                 270         


Val Leu Ala Thr Gly Asn Asp Thr Arg Gly Ala Glu Ala Ser Ala His 
        275                 280                 285             


Ala Tyr Ala Ser Arg Asp Gly Gln Tyr Arg Gly Ile Ala Thr Trp Arg 
    290                 295                 300                 


Tyr Asp Gln Lys Arg Gln Arg Leu Ile Gly Thr Ile Glu Val Pro Met 
305                 310                 315                 320 


Thr Leu Ala Ile Val Gly Gly Gly Thr Lys Val Leu Pro Ile Ala Lys 
                325                 330                 335     


Ala Ser Leu Glu Leu Leu Asn Val Asp Ser Ala Gln Glu Leu Gly His 
            340                 345                 350         


Val Val Ala Ala Val Gly Leu Ala Gln Asn Phe Ala Ala Cys Arg Ala 
        355                 360                 365             


Leu Val Ser Glu Gly Ile Gln Gln Gly His Met Ser Leu Gln Tyr Lys 
    370                 375                 380                 


Ser Leu Ala Ile Val Val Gly Ala Lys Gly Asp Glu Ile Ala Gln Val 
385                 390                 395                 400 


Ala Glu Ala Leu Lys Gln Glu Pro Arg Ala Asn Thr Gln Val Ala Glu 
                405                 410                 415     


Arg Ile Leu Gln Glu Ile Arg Gln Gln 
            420                 425 


<210> 2
<211> 279
<212> PRT
<213> Staphylococcus aureus

<400> 2
Met Ala Val Pro Phe Asn Ala Gly Lys Ile Lys Val Leu Ile Glu Ala 
1               5                   10                  15      


Leu Glu Ser Gly Asn Tyr Ser Ser Ile Lys Ser Asp Val Tyr Asp Gly 
            20                  25                  30          


Met Leu Tyr Asp Ala Pro Asp His Leu Lys Ser Leu Val Asn Arg Phe 
        35                  40                  45              


Val Glu Leu Asn Asn Ile Thr Glu Pro Leu Ala Val Thr Ile Gln Thr 
    50                  55                  60                  


Asn Leu Pro Pro Ser Arg Gly Leu Gly Ser Ser Ala Ala Val Ala Val 
65                  70                  75                  80  


Ala Phe Val Arg Ala Ser Tyr Asp Phe Leu Gly Lys Ser Leu Thr Lys 
                85                  90                  95      


Glu Glu Leu Ile Glu Lys Ala Asn Trp Ala Glu Gln Ile Ala His Gly 
            100                 105                 110         


Lys Pro Ser Gly Ile Asp Thr Gln Thr Ile Val Ser Gly Lys Pro Val 
        115                 120                 125             


Trp Phe Gln Lys Gly His Ala Glu Thr Leu Lys Thr Leu Ser Leu Asp 
    130                 135                 140                 


Gly Tyr Met Val Val Ile Asp Thr Gly Val Lys Gly Ser Thr Arg Gln 
145                 150                 155                 160 


Ala Val Glu Asp Val His Lys Leu Cys Glu Asp Pro Gln Tyr Met Ser 
                165                 170                 175     


His Val Lys His Ile Gly Lys Leu Val Leu Arg Ala Ser Asp Val Ile 
            180                 185                 190         


Glu His His Asn Phe Glu Ala Leu Ala Asp Ile Phe Asn Glu Cys His 
        195                 200                 205             


Ala Asp Leu Lys Ala Leu Thr Val Ser His Asp Lys Ile Glu Gln Leu 
    210                 215                 220                 


Met Lys Ile Gly Lys Glu Asn Gly Ala Ile Ala Gly Lys Leu Thr Gly 
225                 230                 235                 240 


Ala Gly Arg Gly Gly Ser Met Leu Leu Leu Ala Lys Asp Leu Pro Thr 
                245                 250                 255     


Ala Lys Asn Ile Val Lys Ala Val Glu Lys Ala Gly Ala Ala His Thr 
            260                 265                 270         


Trp Ile Glu Asn Leu Gly Gly 
        275                 


<210> 3
<211> 358
<212> PRT
<213> Staphylococcus aureus

<400> 3
Met Ile Gln Val Lys Ala Pro Gly Lys Leu Tyr Ile Ala Gly Glu Tyr 
1               5                   10                  15      


Ala Val Thr Glu Pro Gly Tyr Lys Ser Val Leu Ile Ala Leu Asp Arg 
            20                  25                  30          


Phe Val Thr Ala Thr Ile Glu Glu Ala Asp Gln Tyr Lys Gly Thr Ile 
        35                  40                  45              


His Ser Lys Ala Leu His His Asn Pro Val Thr Phe Ser Arg Asp Glu 
    50                  55                  60                  


Asp Ser Ile Val Ile Ser Asp Pro His Ala Ala Lys Gln Leu Asn Tyr 
65                  70                  75                  80  


Val Val Thr Ala Ile Glu Ile Phe Glu Gln Tyr Ala Lys Ser Cys Asp 
                85                  90                  95      


Ile Ala Met Lys His Phe His Leu Thr Ile Asp Ser Asn Leu Asp Asp 
            100                 105                 110         


Ser Asn Gly His Lys Tyr Gly Leu Gly Ser Ser Ala Ala Val Leu Val 
        115                 120                 125             


Ser Val Ile Lys Val Leu Asn Glu Phe Tyr Asp Met Lys Leu Ser Asn 
    130                 135                 140                 


Leu Tyr Ile Tyr Lys Leu Ala Val Ile Ala Asn Met Lys Leu Gln Ser 
145                 150                 155                 160 


Leu Ser Ser Cys Gly Asp Ile Ala Val Ser Val Tyr Ser Gly Trp Leu 
                165                 170                 175     


Ala Tyr Ser Thr Phe Asp His Glu Trp Val Lys His Gln Ile Glu Asp 
            180                 185                 190         


Thr Thr Val Glu Glu Val Leu Ile Lys Asn Trp Pro Gly Leu His Ile 
        195                 200                 205             


Glu Pro Leu Gln Ala Pro Glu Asn Met Glu Val Leu Ile Gly Trp Thr 
    210                 215                 220                 


Gly Ser Pro Ala Ser Ser Pro His Phe Val Ser Glu Val Lys Arg Leu 
225                 230                 235                 240 


Lys Ser Asp Pro Ser Phe Tyr Gly Asp Phe Leu Glu Asp Ser His Arg 
                245                 250                 255     


Cys Val Glu Lys Leu Ile His Ala Phe Lys Thr Asn Asn Ile Lys Gly 
            260                 265                 270         


Val Gln Lys Met Val Arg Gln Asn Arg Thr Ile Ile Gln Arg Met Asp 
        275                 280                 285             


Lys Glu Ala Thr Val Asp Ile Glu Thr Glu Lys Leu Lys Tyr Leu Cys 
    290                 295                 300                 


Asp Ile Ala Glu Lys Tyr His Gly Ala Ser Lys Thr Ser Gly Ala Gly 
305                 310                 315                 320 


Gly Gly Asp Cys Gly Ile Thr Ile Ile Asn Lys Asp Val Asp Lys Glu 
                325                 330                 335     


Lys Ile Tyr Asp Glu Trp Thr Lys His Gly Ile Lys Pro Leu Lys Phe 
            340                 345                 350         


Asn Ile Tyr His Gly Gln 
        355             


<210> 4
<211> 344
<212> PRT
<213> Streptococcus pneumoniae

<400> 4
Met Tyr His Ser Leu Gly Asn Gln Phe Asp Thr Arg Thr Arg Thr Ser 
1               5                   10                  15      


Arg Lys Ile Arg Arg Glu Arg Ser Cys Ser Asp Met Asp Arg Glu Pro 
            20                  25                  30          


Val Thr Val Arg Ser Tyr Ala Asn Ile Ala Ile Ile Lys Tyr Trp Gly 
        35                  40                  45              


Lys Lys Lys Glu Lys Glu Met Val Pro Ala Thr Ser Ser Ile Ser Leu 
    50                  55                  60                  


Thr Leu Glu Asn Met Tyr Thr Glu Thr Thr Leu Ser Pro Leu Pro Ala 
65                  70                  75                  80  


Asn Val Thr Ala Asp Glu Phe Tyr Ile Asn Gly Gln Leu Gln Asn Glu 
                85                  90                  95      


Val Glu His Ala Lys Met Ser Lys Ile Ile Asp Arg Tyr Arg Pro Ala 
            100                 105                 110         


Gly Glu Gly Phe Val Arg Ile Asp Thr Gln Asn Asn Met Pro Thr Ala 
        115                 120                 125             


Ala Gly Leu Ser Ser Ser Ser Ser Gly Leu Ser Ala Leu Val Lys Ala 
    130                 135                 140                 


Cys Asn Ala Tyr Phe Lys Leu Gly Leu Asp Arg Ser Gln Leu Ala Gln 
145                 150                 155                 160 


Glu Ala Lys Phe Ala Ser Gly Ser Ser Ser Arg Ser Phe Tyr Gly Pro 
                165                 170                 175     


Leu Gly Ala Trp Asp Lys Asp Ser Gly Glu Ile Tyr Pro Val Glu Thr 
            180                 185                 190         


Asp Leu Lys Leu Ala Met Ile Met Leu Val Leu Glu Asp Lys Lys Lys 
        195                 200                 205             


Pro Ile Ser Ser Arg Asp Gly Met Lys Leu Cys Val Glu Thr Ser Thr 
    210                 215                 220                 


Thr Phe Asp Asp Trp Val Arg Gln Ser Glu Lys Asp Tyr Gln Asp Met 
225                 230                 235                 240 


Leu Ile Tyr Leu Lys Glu Asn Asp Phe Ala Lys Ile Gly Glu Leu Thr 
                245                 250                 255     


Glu Lys Asn Ala Leu Ala Met His Ala Thr Thr Lys Thr Ala Ser Pro 
            260                 265                 270         


Ala Phe Ser Tyr Leu Thr Asp Ala Ser Tyr Glu Ala Met Asp Phe Val 
        275                 280                 285             


Arg Gln Leu Arg Glu Lys Gly Glu Ala Cys Tyr Phe Thr Met Asp Ala 
    290                 295                 300                 


Gly Pro Asn Val Lys Val Phe Cys Gln Glu Lys Asp Leu Glu His Leu 
305                 310                 315                 320 


Ser Glu Ile Phe Gly Gln Arg Tyr Arg Leu Ile Val Ser Lys Thr Lys 
                325                 330                 335     


Asp Leu Ser Gln Asp Asp Cys Cys 
            340                 


<210> 5
<211> 327
<212> PRT
<213> Staphylococcus epidermidis

<400> 5
Met Val Lys Ser Gly Lys Ala Arg Ala His Thr Asn Ile Ala Leu Ile 
1               5                   10                  15      


Lys Tyr Trp Gly Lys Ala Asp Glu Thr Tyr Ile Ile Pro Met Asn Asn 
            20                  25                  30          


Ser Leu Ser Val Thr Leu Asp Arg Phe Tyr Thr Glu Thr Lys Val Thr 
        35                  40                  45              


Phe Asp Pro Asp Phe Thr Glu Asp Cys Leu Ile Leu Asn Gly Asn Glu 
    50                  55                  60                  


Val Asn Ala Lys Glu Lys Glu Lys Ile Gln Asn Tyr Met Asn Ile Val 
65                  70                  75                  80  


Arg Asp Leu Ala Gly Asn Arg Leu His Ala Arg Ile Glu Ser Glu Asn 
                85                  90                  95      


Tyr Val Pro Thr Ala Ala Gly Leu Ala Ser Ser Ala Ser Ala Tyr Ala 
            100                 105                 110         


Ala Leu Ala Ala Ala Cys Asn Glu Ala Leu Ser Leu Asn Leu Ser Asp 
        115                 120                 125             


Thr Asp Leu Ser Arg Leu Ala Arg Arg Gly Ser Gly Ser Ala Ser Arg 
    130                 135                 140                 


Ser Ile Phe Gly Gly Phe Ala Glu Trp Glu Lys Gly His Asp Asp Leu 
145                 150                 155                 160 


Thr Ser Tyr Ala His Gly Ile Asn Ser Asn Gly Trp Glu Lys Asp Leu 
                165                 170                 175     


Ser Met Ile Phe Val Val Ile Asn Asn Gln Ser Lys Lys Val Ser Ser 
            180                 185                 190         


Arg Ser Gly Met Ser Leu Thr Arg Asp Thr Ser Arg Phe Tyr Gln Tyr 
        195                 200                 205             


Trp Leu Asp His Val Asp Glu Asp Leu Asn Glu Ala Lys Glu Ala Val 
    210                 215                 220                 


Lys Asn Gln Asp Phe Gln Arg Leu Gly Glu Val Ile Glu Ala Asn Gly 
225                 230                 235                 240 


Leu Arg Met His Ala Thr Asn Leu Gly Ala Gln Pro Pro Phe Thr Tyr 
                245                 250                 255     


Leu Val Gln Glu Ser Tyr Asp Ala Met Ala Ile Val Glu Gln Cys Arg 
            260                 265                 270         


Lys Ala Asn Leu Pro Cys Tyr Phe Thr Met Asp Ala Gly Pro Asn Val 
        275                 280                 285             


Lys Val Leu Val Glu Lys Lys Asn Lys Gln Ala Val Met Glu Gln Phe 
    290                 295                 300                 


Leu Lys Val Phe Asp Glu Ser Lys Ile Ile Ala Ser Asp Ile Ile Ser 
305                 310                 315                 320 


Ser Gly Val Glu Ile Ile Lys 
                325         


<210> 6
<211> 176
<212> PRT
<213> Burkholderia multivorans

<400> 6
Met Glu Glu Arg Leu Ile Leu Val Asp Thr Asp Asp Arg Pro Ile Gly 
1               5                   10                  15      


Ile Cys Glu Lys Met Arg Ala His His Glu Gly Leu Leu His Arg Ala 
            20                  25                  30          


Phe Ser Ile Phe Val Phe Asp Ser Ala Gly Arg Leu Leu Leu Gln Gln 
        35                  40                  45              


Arg Ala Leu Asn Lys Tyr His Ser Gly Gly Leu Trp Ser Asn Thr Cys 
    50                  55                  60                  


Cys Gly His Pro Arg Pro Arg Glu Ala Leu Pro Asp Ala Val Arg Arg 
65                  70                  75                  80  


Arg Leu Gly Glu Glu Met Gly Phe Ala Cys Glu Leu Arg Pro Val Asp 
                85                  90                  95      


Ala Leu Val Tyr Arg Ala Arg Phe Glu Asn Asp Leu Ile Glu His Glu 
            100                 105                 110         


Phe Val His Ile His Val Gly Arg Phe Asp Gly Thr Val Ala Pro Asp 
        115                 120                 125             


Phe Ala Glu Val Ala Ala Trp Arg Trp Ile Asp Val Pro Thr Leu Leu 
    130                 135                 140                 


Glu Trp Met Ala Asp Glu Pro Ser Ala Phe Thr Val Trp Phe His Cys 
145                 150                 155                 160 


Met Ile Glu Arg Ala Gly Leu Pro Val Leu His Arg Trp Ala His Arg 
                165                 170                 175     


<210> 7
<211> 594
<212> PRT
<213> Mucuna pruriens

<400> 7
Met Ala Thr Asn Pro Ser Cys Leu Ser Thr Pro Phe Leu Ser Ser Thr 
1               5                   10                  15      


Pro Ala Leu Ser Thr Arg Phe Pro Leu Ser Glu Asn Phe Thr Gln Lys 
            20                  25                  30          


Thr Ser Leu Val Asn Pro Lys Pro Trp Pro Leu Ile Ser Ala Val Ser 
        35                  40                  45              


Ser Gln Phe Ser Gln Ile Ala Glu Asp Asn Ser Arg Arg Ser Ala Asn 
    50                  55                  60                  


Tyr His Pro Asn Leu Trp Asp Phe Glu Phe Leu Gln Ser Leu Glu Asn 
65                  70                  75                  80  


Asp Ser Lys Met Glu Lys Leu Glu Glu Lys Ala Thr Lys Leu Glu Glu 
                85                  90                  95      


Glu Val Arg Asn Met Met Asn Glu Ala Lys Thr Glu Ala Leu Ser Leu 
            100                 105                 110         


Leu Glu Leu Ile Asp Asp Val Gln Arg Leu Gly Leu Thr Tyr Lys Phe 
        115                 120                 125             


Glu Lys Asp Ile Ile Lys Ala Leu Glu Lys Ile Val Pro Leu Asp Glu 
    130                 135                 140                 


Ser Gly Leu His Val Thr Ser Leu Ser Phe Arg Ile Leu Arg Gln His 
145                 150                 155                 160 


Gly Phe Glu Val Ser Gln Asp Val Phe Lys Arg Phe Lys Asp Lys Glu 
                165                 170                 175     


Gly Gly Phe Cys Ala Glu Leu Lys Asp Asp Val Gln Gly Leu Leu Ser 
            180                 185                 190         


Leu Tyr Glu Ala Ser Tyr Leu Gly Phe Glu Gly Glu Ser Leu Leu Asp 
        195                 200                 205             


Glu Ala Arg Ala Phe Ser Ile Thr His Leu Lys Asn Asn Leu Asn Lys 
    210                 215                 220                 


Gly Ile Asn Thr Lys Val Ala Gln Gln Val Ser His Ala Leu Glu Leu 
225                 230                 235                 240 


Pro Tyr His Arg Arg Leu His Arg Leu Glu Ala Arg Trp Leu Leu Asp 
                245                 250                 255     


Lys Tyr Glu Pro Lys Glu Pro His His His Leu Leu His Glu Leu Ala 
            260                 265                 270         


Lys Leu Asp Phe Asn Leu Val Gln Ser Leu Tyr Gln Lys Glu Leu Arg 
        275                 280                 285             


Glu Leu Ser Leu Trp Trp Arg Glu Ile Gly Leu Thr Ser Lys Leu Asp 
    290                 295                 300                 


Phe Val Arg Asp Arg Leu Met Glu Val Tyr Phe Trp Ala Leu Gly Met 
305                 310                 315                 320 


Ala Pro Asp Pro Gln Phe Ser Glu Cys Arg Lys Val Val Thr Lys Met 
                325                 330                 335     


Phe Gly Leu Val Thr Ile Ile Asp Asp Val Tyr Asp Val Tyr Gly Thr 
            340                 345                 350         


Leu Asp Glu Leu Gln Leu Phe Thr Asp Ala Val Glu Arg Trp Asp Val 
        355                 360                 365             


Asn Ala Ile Asn Thr Leu Pro Asp Tyr Met Lys Leu Cys Tyr Leu Ala 
    370                 375                 380                 


Leu Tyr Asn Thr Val Asn Asp Thr Ala Tyr Ser Ile Leu Lys Glu Lys 
385                 390                 395                 400 


Gly His Asn Asn Ile Ser Tyr Leu Thr Lys Ser Trp Cys Glu Leu Cys 
                405                 410                 415     


Lys Ala Phe Leu Gln Glu Ala Lys Trp Ser Asn Asn Lys Ile Ile Pro 
            420                 425                 430         


Ala Phe Asn Lys Tyr Leu Asp Asn Ala Ser Val Ser Ser Ser Gly Val 
        435                 440                 445             


Ala Leu Leu Ala Pro Ser Tyr Phe Leu Val Cys Gln Glu Gln Asp Ile 
    450                 455                 460                 


Ser Asp Gln Ala Leu His Ser Leu Thr Asn Phe His Gly Leu Val Arg 
465                 470                 475                 480 


Ser Ser Cys Thr Ile Phe Arg Leu Cys Asn Asp Leu Ala Thr Ser Ser 
                485                 490                 495     


Ala Glu Leu Glu Arg Gly Glu Thr Thr Asn Ser Ile Thr Ser Tyr Met 
            500                 505                 510         


His Glu Asn Glu Thr Ser Glu Glu Gln Ala Cys Lys Glu Leu Arg Asn 
        515                 520                 525             


Leu Ile Asp Ala Glu Trp Lys Lys Met Asn Glu Glu Arg Val Ser Asn 
    530                 535                 540                 


Ser Thr Leu Pro Lys Ala Phe Arg Glu Ile Ala Ile Asn Met Ala Arg 
545                 550                 555                 560 


Ile Ser His Cys Thr Tyr Gln Tyr Gly Asp Gly Leu Gly Arg Pro Asp 
                565                 570                 575     


Tyr Thr Thr Glu Asn Arg Ile Lys Leu Leu Leu Ile Asp Pro Phe Pro 
            580                 585                 590         


Ile Asn 
        


<210> 8
<211> 1278
<212> DNA
<213> Staphylococcus aureus

<400> 8
ctattgttgt ctaatttctt gtaaaatgcg ttcagctact tgtgtattcg cacggggttc       60

ttgcttcaat gcttcagcta cttgcgcaat ttcatcacct tttgcaccta caacaatagc      120

taaagattta tattgcaagc tcatatggcc ttgctggata ccttcggaaa cgagcgcgcg      180

acatgctgca aagttctgtg ctaaaccaac ggcagcaact acatgaccta attcttgtgc      240

tgaatctaca tttagcaatt ctaaagaagc tttagcaatt ggtaatactt ttgtaccacc      300

gccaacgatt gccaatgtca taggcacttc tattgtacca attaaacgtt gacgtttttg      360

atcgtatctc catgttgcaa taccacgata ctgtccgtca cgactcgcgt atgcatgcgc      420

acttgcttct gcaccacgcg tatcatttcc tgttgctaaa acaacggcat gtatgccatt      480

cataacacct ttattatgtg ttgcagcacg atgaatatca acttgtgcca atacagaagc      540

acgttccatt cgtttggcaa cctcttctcc agttctctcg ccccttgcta aatctttaac      600

gtcaatttcg ccttgaactt taacaacgga cgctgttgca tgattggata aaatactcat      660

taaaatgtcg ctttgtggag attcattttt taaaaatgca gttatggcct ctaaaatcgt      720

attaagcata ttagcgccca tagcatcttt cgtatcaaca aatactttta aagatagtaa      780

ctgttgctca ggaaatgtat caatagctat acgttggtaa ccaccaccac gcgctttaat      840

agaaggatat gcctcatccg caattttatg aatttgcttt tctaaagctt taatgtctgc      900

tgataatttt tcagtatcgt caacgccatc aaagacgatt tgacctatca taatacgttc      960

agaagatacc gttttaaatc cgccagtctg attcactagc tttgcaccat aactagctgc     1020

agcgacaact gaaggctctt ccaccatcat aggtacaaca tatgccttat cgtccacaat     1080

gatattcggt aataatccaa cgggtaatgc accttgcgcg atgacatttt caattaaact     1140

atttgctact tcctcatcaa ttaatggatg attcaataaa atgtcgaatt gatcttctga     1200

taaccattgc ttatctacca attgttgtaa cttttgttga cgagataaat gtcggaaatt     1260

cttatctaaa ctttgcat                                                   1278


<210> 9
<211> 840
<212> DNA
<213> Staphylococcus aureus

<400> 9
attgcagtac cgtttaacgc aggtaaaatc aaagttttaa tagaagcctt agagagcggg       60

aactattcgt ctattaaaag cgatgtttac gatggtatgt tatatgatgc gcctgaccat      120

cttaagtctt tggtgaaccg ttttgtagaa ttaaataata ttacagagcc gctagcagta      180

acgatccaaa cgaatttacc accatcacgt ggattaggat cgagtgcagc tgtcgcggtt      240

gcttttgttc gtgcaagtta tgatttttta gggaaatcat taacgaaaga agaactcatt      300

gaaaaggcta attgggcaga gcaaattgca catggtaaac caagtggtat tgatacgcaa      360

acgattgtat caggcaaacc agtttggttc caaaaaggtc atgctgaaac attgaaaacg      420

ttaagtttag acggctatat ggttgttatt gatactggtg tgaaaggttc aacaagacaa      480

gcggtagaag atgttcataa actttgtgag gatcctcagt acatgtcaca tgtaaaacat      540

atcggtaagt tagttttacg tgcgagtgat gtgattgaac atcataactt tgaagcccta      600

gcggatattt ttaatgaatg tcatgcggat ttaaaggcgt tgacagttag tcatgataaa      660

atagaacaat taatgaaaat tggtaaagaa aatggtgcga ttgctggaaa acttactggt      720

gctggtcgtg gtggaagtat gttattgctt gccaaagatt taccaacagc gaaaaatatt      780

gtgaaagctg tagaaaaagc tggtgcagca catacatgga ttgagaattt aggaggttaa      840


<210> 10
<211> 1077
<212> DNA
<213> Staphylococcus aureus

<400> 10
atgattcagg tcaaagcacc cggaaaactt tatattgctg gagaatatgc tgtaacagaa       60

ccaggatata aatctgtact tattgcgtta gatcgttttg taactgctac tattgaagaa      120

gcagaccaat ataaaggtac cattcattca aaagcattac atcataaccc agttacattt      180

agtagagatg aagatagtat tgtcatttca gatccacatg cagcaaaaca attaaattat      240

gtggtcacag ctattgaaat atttgaacaa tacgcgaaaa gttgcgatat agcgatgaag      300

cattttcatc tgactattga tagtaattta gatgattcaa atggtcataa atatggatta      360

ggttcaagtg cagcagtact tgtgtcagtt ataaaagtat taaatgaatt ttatgatatg      420

aagttatcta atttatacat ttataaacta gcagtgattg caaatatgaa gttacaaagt      480

ttaagttcat gcggagatat tgctgtgagt gtatatagtg gatggttagc gtatagtact      540

tttgatcatg aatgggttaa gcatcaaatt gaagatacta cggttgaaga agttttaatc      600

aaaaactggc ctggattgca catcgaacca ttacaagcac ctgaaaatat ggaagtactt      660

atcggttgga ctggctcacc ggcgtcatca ccacactttg ttagcgaagt gaaacgtttg      720

aaatcagatc cttcatttta cggtgacttc ttagaagatt cacatcgttg tgttgaaaag      780

cttattcatg cttttaaaac aaataacatt aaaggtgtgc aaaagatggt gcgtcagaat      840

cgtacaatta ttcaacgtat ggataaagaa gctacagttg atatagaaac tgaaaagcta      900

aaatatttgt gtgatattgc tgaaaagtat cacggtgcat ctaaaacatc aggcgctggt      960

ggtggagact gtggtattac aattatcaat aaagatgtag ataaagaaaa aatttatgat     1020

gaatggacaa aacatggtat taaaccatta aaatttaata tttatcatgg gcaataa        1077


<210> 11
<211> 1035
<212> DNA
<213> Streptococcus pneumoniae

<400> 11
ttgtatcata gccttggtaa ccaatttgac acacgcacaa gaactagcag aaagattaga       60

agagaaagga gctgttcaga catggataga gagcctgtga cagtacgttc ctacgcaaat      120

attgctatta tcaaatattg gggaaagaaa aaagaaaaag agatggtgcc tgctactagc      180

agtatttctc taactttgga aaatatgtat acagagacga ccttgtcgcc tttaccagcc      240

aatgtaacag ctgacgaatt ttacatcaat ggtcagctac aaaatgaggt cgagcatgcc      300

aagatgagta agattattga ccgttatcgt ccagctggtg agggctttgt ccgtatcgat      360

actcaaaaca atatgcctac ggcagcgggc ctgtcctcaa gttctagtgg tttgtccgcc      420

ctggtcaagg cttgtaatgc ttatttcaag cttggattgg atagaagtca gttagcgcag      480

gaagccaagt ttgcctcagg ctcttcttct cggagttttt atggaccact aggagcctgg      540

gataaggata gtggagaaat ttaccctgta gagacagact tgaaactagc tatgattatg      600

ttggtgctag aggacaagaa aaaaccaatc tctagccgtg acgggatgaa actttgtgtg      660

gaaacctcga cgactttcga cgactgggtt cgtcagtctg agaaggacta tcaggatatg      720

ctgatttatc tcaaggaaaa tgattttgcc aagattggag aattaacgga gaaaaatgcc      780

ctggctatgc atgctacgac aaagactgct agtccagcct tttcttatct gacggatgcc      840

tcttatgagg ctatggactt tgttcgtcag cttcgtgaga aaggagaggc ctgctacttt      900

accatggatg ctggtcccaa tgttaaggtc ttctgtcagg agaaagactt ggagcatttg      960

tcagaaattt tcggtcagcg ttatcgcttg attgtgtcaa aaacaaagga tttgagtcaa     1020

gatgattgct gttaa                                                      1035


<210> 12
<211> 531
<212> DNA
<213> Burkholderia multivorans

<400> 12
tcatctgtgt gcccagcgat gcagcactgg caatccggct cgctctatca tgcagtggaa       60

ccagacagta aaagcgctcg gctcgtccgc catccactcc agcaaggttg gcacatcgat      120

ccatcgccac gccgctactt ccgcgaaatc tggggcgacc gttccatcga accgaccaac      180

atgaatatgc acaaactcgt gctcgatcag gtcgttctca aatctcgcgc ggtacacgag      240

cgcgtccacg ggccgaagtt cacatgcgaa tcccatttct tcgccaagcc ggcggcgaac      300

cgcatcaggc agcgcttcgc gtggacgcgg gtgcccgcag catgtgttgg accacagccc      360

gcccgagtgg tacttattca gcgcacgctg ctgtagcagc aagcgaccgg ccgagtcgaa      420

cacaaaaatc gagaatgcgc ggtgcagcag cccttcatgg tgcgcgcgca tcttctcgca      480

tattcctatc ggtcgatcgt cggtatcgac gaggatcagg cgttcttcca t               531


<210> 13
<211> 1785
<212> DNA
<213> Mucuna pruriens

<400> 13
atggcaacca acccttcatg cttatctact ccatttttgt cctccacacc agcactaagt       60

actagatttc cattaagtga gaacttcaca caaaaaacat ctcttgtcaa tcccaaacct      120

tggccactta tttctgcagt cagctctcaa tttagccaaa tagcagaaga taatagtcgt      180

cgttcagcta attaccaccc aaacctctgg gattttgaat ttctgcagtc tctcgaaaat      240

gactctaaga tggaaaagct ggaagagaaa gcaacaaagt tggaggagga agtgcgaaac      300

atgatgaacg aagcaaagac agaagcacta agcttattgg aattgataga cgacgtccag      360

cgtctgggat tgacctacaa gtttgagaag gacataatca aagcccttga gaagattgtt      420

ccattggatg agagtgggct gcatgttact tctctcagct tccgtatact tagacaacat      480

ggctttgagg tttcccaaga tgtgtttaag agatttaagg acaaggaggg aggtttttgt      540

gctgaactta aagacgatgt tcaagggttg ctaagtctat atgaagcatc ctatcttggt      600

tttgagggag aaagtctctt agacgaggca agggcatttt caataacaca tctcaagaac      660

aacctaaaca aaggaataaa caccaaagta gcccaacaag ttagccatgc actggaactt      720

ccttatcatc gaagactgca tagactggaa gcacgatggc tccttgacaa atatgaacca      780

aaggaacccc accatcattt actacacgag cttgcaaagt tggatttcaa tttggtccaa      840

tcattgtacc agaaagagtt gcgagaattg tcactgtggt ggagggagat tgggctcaca      900

agcaagttgg actttgttcg agacagatta atggaagtgt acttttgggc gctgggaatg      960

gcacctgatc ctcaatttag tgaatgtcgt aaagtcgtca ctaaaatgtt tgggctagtt     1020

actatcatcg atgatgtata tgacgtttac ggtactttgg acgagctaca actcttcacc     1080

gatgctgttg agagatggga cgtgaatgcg ataaatacac ttccagacta tatgaaattg     1140

tgctatttag ccctttataa caccgtcaat gacacagctt atagcatcct taaagaaaag     1200

ggacataaca acatttctta tttgacaaaa tcttggtgtg agttgtgcaa agcattcctc     1260

caagaagcaa aatggtcaaa caacaaaatc attccagcat tcaacaagta cctagacaat     1320

gcatcggtgt cctcctctgg tgtggctttg cttgctcctt cctacttctt agtgtgccaa     1380

gaacaagaca tttcagacca agctcttcat tccttaacta atttccatgg ccttgtgcgt     1440

tcatcatgca ccatttttag gctttgcaat gatctggcta cctcatcggc tgagctagag     1500

agaggtgaaa caacaaattc aatcacatcg tacatgcatg agaatgagac ttctgaggag     1560

caagcatgta aggagttgag aaatttgatc gatgcagagt ggaagaagat gaatgaagag     1620

cgagtttcaa attctacact cccaaaagca tttagggaaa tagctattaa catggctcgg     1680

atttcccatt gcacatacca atatggagac ggacttggaa ggcccgacta caccacagag     1740

aacaggataa agttgctact aatagaccct tttccaatta attag                     1785


<210> 14
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 14
His His His His His His 
1               5       


