                         SEQUENCE LISTING

<110>  RESEARCH INSTITUE AT NATIONWIDE CHILDREN'S HOSPITAL
 
<120>  RESPIRATORY SYNCYTIAL VIRUS HAVING ALTERED NS1 PROTEIN FUNCTION 
       AND RELATED MATERIALS AND METHODS

<130>  509892.10

<150>  US 62/205,627
<151>  2015-08-14

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  139
<212>  PRT
<213>  Human respiratory syncytial virus; NS1 protein sequence of human RSV strain A2

<400>  1

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Arg Leu Gln Asn Leu 
1               5                   10                  15      


Phe Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp 
            20                  25                  30          


Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr 
        35                  40                  45              


Ile Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp 
    50                  55                  60                  


Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met 
65                  70                  75                  80  


Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr 
                85                  90                  95      


His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys 
            100                 105                 110         


Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln 
        115                 120                 125             


Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135                 


<210>  2
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant Nd10 (deletion of amino-terminal 10 aa)

<400>  2

Val Arg Leu Gln Asn Leu Phe Asp Asn Asp Glu Val Ala Leu Leu Lys 
1               5                   10                  15      


Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala 
            20                  25                  30          


Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His 
        35                  40                  45              


Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys 
    50                  55                  60                  


Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp 
65                  70                  75                  80  


Glu Met Met Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp 
                85                  90                  95      


Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met 
            100                 105                 110         


Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn 
        115                 120                 125             


Pro 
    


<210>  3
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant Cd10 (deletion of carboxy-terminal 10 aa)

<400>  3

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Arg Leu Gln Asn Leu 
1               5                   10                  15      


Phe Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp 
            20                  25                  30          


Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr 
        35                  40                  45              


Ile Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp 
    50                  55                  60                  


Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met 
65                  70                  75                  80  


Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr 
                85                  90                  95      


His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys 
            100                 105                 110         


Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln 
        115                 120                 125             


Leu 
    


<210>  4
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d6-15 (deletion of aa 6 through 15)

<400>  4

Met Gly Ser Asn Ser Leu Phe Asp Asn Asp Glu Val Ala Leu Leu Lys 
1               5                   10                  15      


Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala 
            20                  25                  30          


Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His 
        35                  40                  45              


Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys 
    50                  55                  60                  


Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp 
65                  70                  75                  80  


Glu Met Met Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp 
                85                  90                  95      


Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met 
            100                 105                 110         


Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn 
        115                 120                 125             


Pro 
    


<210>  5
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d6-18 (deltion of aa 6 through 18)

<400>  5

Met Gly Ser Asn Ser Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys 
1               5                   10                  15      


Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val 
            20                  25                  30          


Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr 
        35                  40                  45              


Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe 
    50                  55                  60                  


Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met 
65                  70                  75                  80  


Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys 
                85                  90                  95      


Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr 
            100                 105                 110         


Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
        115                 120                 125     


<210>  6
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d8-18 (deletion of aa 8 through 18)

<400>  6

Met Gly Ser Asn Ser Leu Ser Asn Asp Glu Val Ala Leu Leu Lys Ile 
1               5                   10                  15      


Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys 
            20                  25                  30          


Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His Val 
        35                  40                  45              


Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser 
    50                  55                  60                  


Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu 
65                  70                  75                  80  


Met Met Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp 
                85                  90                  95      


Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr 
            100                 105                 110         


Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
        115                 120                 125             


<210>  7
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d8-20 (deletion of aa 8 through 20

<400>  7

Met Gly Ser Asn Ser Leu Ser Glu Val Ala Leu Leu Lys Ile Thr Cys 
1               5                   10                  15      


Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val 
            20                  25                  30          


Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr 
        35                  40                  45              


Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe 
    50                  55                  60                  


Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met 
65                  70                  75                  80  


Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys 
                85                  90                  95      


Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr 
            100                 105                 110         


Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
        115                 120                 125     


<210>  8
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d11-20 (deletion of aa 11 through 20)

<400>  8

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Glu Val Ala Leu Leu Lys 
1               5                   10                  15      


Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala 
            20                  25                  30          


Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His 
        35                  40                  45              


Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys 
    50                  55                  60                  


Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp 
65                  70                  75                  80  


Glu Met Met Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp 
                85                  90                  95      


Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met 
            100                 105                 110         


Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn 
        115                 120                 125             


Pro 
    


<210>  9
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d6-10,12,15-19 (deletion of aa 6 through 
       10, 12, and 15 through 19)

<400>  9

Met Gly Ser Asn Ser Val Leu Gln Asp Glu Val Ala Leu Leu Lys Ile 
1               5                   10                  15      


Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys 
            20                  25                  30          


Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile Val Phe Val His Val 
        35                  40                  45              


Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser 
    50                  55                  60                  


Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu 
65                  70                  75                  80  


Met Met Glu Leu Thr His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp 
                85                  90                  95      


Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr 
            100                 105                 110         


Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
        115                 120                 125             


<210>  10
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant Nd5 (deletion of aa 1 through 5)

<400>  10

Leu Ser Met Ile Lys Val Arg Leu Gln Asn Leu Phe Asp Asn Asp Glu 
1               5                   10                  15      


Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu 
            20                  25                  30          


Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly 
        35                  40                  45              


Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn 
    50                  55                  60                  


Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn 
65                  70                  75                  80  


Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro 
                85                  90                  95      


Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu 
            100                 105                 110         


Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu 
        115                 120                 125             


Gly Phe Asp Leu Asn Pro 
    130                 


<210>  11
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d2-5 (deletion of aa 2 through 5

<400>  11

Met Leu Ser Met Ile Lys Val Arg Leu Gln Asn Leu Phe Asp Asn Asp 
1               5                   10                  15      


Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His 
            20                  25                  30          


Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn 
        35                  40                  45              


Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn 
    50                  55                  60                  


Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln 
65                  70                  75                  80  


Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln 
                85                  90                  95      


Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys 
            100                 105                 110         


Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu 
        115                 120                 125             


Leu Gly Phe Asp Leu Asn Pro 
    130                 135 


<210>  12
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d2-7 (deletion of aa 2 through 7)

<400>  12

Met Met Ile Lys Val Arg Leu Gln Asn Leu Phe Asp Asn Asp Glu Val 
1               5                   10                  15      


Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr 
            20                  25                  30          


Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile 
        35                  40                  45              


Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn 
    50                  55                  60                  


Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly 
65                  70                  75                  80  


Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro Asn 
                85                  90                  95      


Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser 
            100                 105                 110         


Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly 
        115                 120                 125             


Phe Asp Leu Asn Pro 
    130             


<210>  13
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d5-7 (deletion of aa 5 through 7)

<400>  13

Met Gly Ser Asn Met Ile Lys Val Arg Leu Gln Asn Leu Phe Asp Asn 
1               5                   10                  15      


Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile 
            20                  25                  30          


His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu 
        35                  40                  45              


Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro 
    50                  55                  60                  


Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu 
65                  70                  75                  80  


Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser 
                85                  90                  95      


Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys 
            100                 105                 110         


Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu 
        115                 120                 125             


Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135     


<210>  14
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d6-10 (deletion of aa 6 through 10)

<400>  14

Met Gly Ser Asn Ser Val Arg Leu Gln Asn Leu Phe Asp Asn Asp Glu 
1               5                   10                  15      


Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu 
            20                  25                  30          


Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly 
        35                  40                  45              


Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn 
    50                  55                  60                  


Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn 
65                  70                  75                  80  


Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro 
                85                  90                  95      


Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu 
            100                 105                 110         


Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu 
        115                 120                 125             


Gly Phe Asp Leu Asn Pro 
    130                 


<210>  15
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d11-15 (deletion of aa 11 through 15)

<400>  15

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Leu Phe Asp Asn Asp Glu 
1               5                   10                  15      


Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu 
            20                  25                  30          


Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly 
        35                  40                  45              


Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn 
    50                  55                  60                  


Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn 
65                  70                  75                  80  


Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro 
                85                  90                  95      


Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu 
            100                 105                 110         


Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu 
        115                 120                 125             


Gly Phe Asp Leu Asn Pro 
    130                 


<210>  16
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d16-20 (deletion of aa 16 through 20)

<400>  16

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Arg Leu Gln Asn Glu 
1               5                   10                  15      


Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu 
            20                  25                  30          


Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly 
        35                  40                  45              


Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn 
    50                  55                  60                  


Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn 
65                  70                  75                  80  


Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro 
                85                  90                  95      


Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu 
            100                 105                 110         


Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu 
        115                 120                 125             


Gly Phe Asp Leu Asn Pro 
    130                 


<210>  17
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d11-13,18-20 (deletion of aa 11 through 
       13 and 18 through 20)

<400>  17

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Gln Asn Leu Phe Glu Val 
1               5                   10                  15      


Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile His Leu Thr 
            20                  25                  30          


Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu Asn Gly Ile 
        35                  40                  45              


Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro Asn Asn Asn 
    50                  55                  60                  


Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu Gln Asn Gly 
65                  70                  75                  80  


Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser Gln Pro Asn 
                85                  90                  95      


Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys Lys Leu Ser 
            100                 105                 110         


Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu Leu Leu Gly 
        115                 120                 125             


Phe Asp Leu Asn Pro 
    130             


<210>  18
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant Cd5 (deletion of aa 135-139)

<400>  18

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Arg Leu Gln Asn Leu 
1               5                   10                  15      


Phe Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp 
            20                  25                  30          


Lys Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr 
        35                  40                  45              


Ile Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp 
    50                  55                  60                  


Ile Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met 
65                  70                  75                  80  


Pro Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr 
                85                  90                  95      


His Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys 
            100                 105                 110         


Phe Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln 
        115                 120                 125             


Leu Ser Glu Leu Leu Gly 
    130                 


<210>  19
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d9 (deletion of aa 9)

<400>  19

Met Gly Ser Asn Ser Leu Ser Met Lys Val Arg Leu Gln Asn Leu Phe 
1               5                   10                  15      


Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys 
            20                  25                  30          


Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile 
        35                  40                  45              


Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile 
    50                  55                  60                  


Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro 
65                  70                  75                  80  


Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His 
                85                  90                  95      


Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe 
            100                 105                 110         


Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu 
        115                 120                 125             


Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135             


<210>  20
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d11 (deletion of aa 11)

<400>  20

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Arg Leu Gln Asn Leu Phe 
1               5                   10                  15      


Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys 
            20                  25                  30          


Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile 
        35                  40                  45              


Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile 
    50                  55                  60                  


Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro 
65                  70                  75                  80  


Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His 
                85                  90                  95      


Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe 
            100                 105                 110         


Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu 
        115                 120                 125             


Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135             


<210>  21
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d12 (deletion of aa 12)

<400>  21

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Leu Gln Asn Leu Phe 
1               5                   10                  15      


Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys 
            20                  25                  30          


Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile 
        35                  40                  45              


Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile 
    50                  55                  60                  


Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro 
65                  70                  75                  80  


Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His 
                85                  90                  95      


Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe 
            100                 105                 110         


Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu 
        115                 120                 125             


Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135             


<210>  22
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d13 (deletion of aa 13)

<400>  22

Met Gly Ser Asn Ser Leu Ser Met Ile Lys Val Arg Gln Asn Leu Phe 
1               5                   10                  15      


Asp Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys 
            20                  25                  30          


Leu Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile 
        35                  40                  45              


Lys Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile 
    50                  55                  60                  


Cys Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro 
65                  70                  75                  80  


Val Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His 
                85                  90                  95      


Cys Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe 
            100                 105                 110         


Ser Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu 
        115                 120                 125             


Ser Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135             


<210>  23
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d7,8 (deletion of aa 7 and 8)

<400>  23

Met Gly Ser Asn Ser Leu Ile Lys Val Arg Leu Gln Asn Leu Phe Asp 
1               5                   10                  15      


Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu 
            20                  25                  30          


Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys 
        35                  40                  45              


Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys 
    50                  55                  60                  


Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val 
65                  70                  75                  80  


Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys 
                85                  90                  95      


Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser 
            100                 105                 110         


Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser 
        115                 120                 125             


Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135         


<210>  24
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d9-11 (deletion of aa 9, 10, and 11)

<400>  24

Met Gly Ser Asn Ser Leu Ser Met Arg Leu Gln Asn Leu Phe Asp Asn 
1               5                   10                  15      


Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu Ile 
            20                  25                  30          


His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys Leu 
        35                  40                  45              


Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys Pro 
    50                  55                  60                  


Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val Leu 
65                  70                  75                  80  


Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys Ser 
                85                  90                  95      


Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser Lys 
            100                 105                 110         


Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser Glu 
        115                 120                 125             


Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135     


<210>  25
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial sequence based on human RSV NS1 protein (SEQ ID NO: 
       1); NS1 deletion mutant d9,12 (deletion of aa 9 and 12)

<400>  25

Met Gly Ser Asn Ser Leu Ser Met Lys Val Leu Gln Asn Leu Phe Asp 
1               5                   10                  15      


Asn Asp Glu Val Ala Leu Leu Lys Ile Thr Cys Tyr Thr Asp Lys Leu 
            20                  25                  30          


Ile His Leu Thr Asn Ala Leu Ala Lys Ala Val Ile His Thr Ile Lys 
        35                  40                  45              


Leu Asn Gly Ile Val Phe Val His Val Ile Thr Ser Ser Asp Ile Cys 
    50                  55                  60                  


Pro Asn Asn Asn Ile Val Val Lys Ser Asn Phe Thr Thr Met Pro Val 
65                  70                  75                  80  


Leu Gln Asn Gly Gly Tyr Ile Trp Glu Met Met Glu Leu Thr His Cys 
                85                  90                  95      


Ser Gln Pro Asn Gly Leu Leu Asp Asp Asn Cys Glu Ile Lys Phe Ser 
            100                 105                 110         


Lys Lys Leu Ser Asp Ser Thr Met Thr Asn Tyr Met Asn Gln Leu Ser 
        115                 120                 125             


Glu Leu Leu Gly Phe Asp Leu Asn Pro 
    130                 135         


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  26
ctaactgcaa cctttcgaag c                                                 21


<210>  27
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthtetic primer

<400>  27
ggaaagagct gtagtggaga ag                                                22


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  28
gtaacccgtt gaaccccatt                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  29
ccatccaatc ggtagtagcg                                                   20


