
                                SEQUENCE LISTING

<110> Massachusetts Institute of Technology
      Ouyang, Wei
      Han, Jongyoon

<120> Microfluidic Platform for Rapid
  Biologics Analysis Using Molecular Charge Modulation and
  Electrokinetic Concentration Based Receptor Assays
  

<130> 0050.2278001

<140> PCT/US2016/047567   
<141> 2016-08-18  

<150> 62/206,819          
<151> 2015-08-18  

<160> 8

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 34
<212> PRT
<213> Artificial Sequence

<220> 
<223> Positive Charge Modulator

<400> 1
Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg
 1               5                  10                  15      
Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg Gly Arg
            20                  25                  30          
Gly Lys
        


<210> 2
<211> 64
<212> PRT
<213> Artificial Sequence

<220> 
<223> Negative Charge Modulator

<400> 2
Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr
 1               5                  10                  15      
Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr
            20                  25                  30          
Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr
        35                  40                  45              
Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr Ala Gly Cys Thr
    50                  55                  60                  


<210> 3
<211> 192
<212> PRT
<213> Homo sapien

<400> 3
Met Phe Pro Thr Ile Pro Leu Ser Arg Leu Phe Asp Asn Ala Met Leu
 1               5                  10                  15      
Arg Ala His Arg Leu His Gln Leu Ala Phe Asp Thr Tyr Gln Glu Phe
            20                  25                  30          
Glu Glu Ala Tyr Ile Pro Lys Glu Gln Lys Tyr Ser Phe Leu Gln Asn
        35                  40                  45              
Pro Gln Thr Ser Leu Cys Phe Ser Glu Ser Ile Pro Thr Pro Ser Asn
    50                  55                  60                  
Arg Glu Glu Thr Gln Gln Lys Ser Asn Leu Glu Leu Leu Arg Ile Ser
65                  70                  75                  80  
Leu Leu Leu Ile Gln Ser Trp Leu Glu Pro Val Gln Phe Leu Arg Ser
                85                  90                  95      
Val Phe Ala Asn Ser Leu Val Tyr Gly Ala Ser Asp Ser Asn Val Tyr
            100                 105                 110         
Asp Leu Leu Lys Asp Leu Glu Glu Gly Ile Gln Thr Leu Met Gly Arg
        115                 120                 125             
Leu Glu Asp Gly Ser Pro Arg Thr Gly Gln Ile Phe Lys Gln Thr Tyr
    130                 135                 140                 
Ser Lys Phe Asp Thr Asn Ser His Asn Asp Asp Ala Leu Leu Lys Asn
145                 150                 155                 160 
Tyr Gly Leu Leu Tyr Cys Phe Arg Lys Asp Met Asp Lys Val Glu Thr
                165                 170                 175     
Phe Leu Arg Ile Val Gln Cys Arg Ser Val Glu Gly Ser Cys Gly Phe
            180                 185                 190         


<210> 4
<211> 165
<212> PRT
<213> Homo sapien

<400> 4
Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met
 1               5                  10                  15      
Leu Leu Ala Gln Met Arg Arg Ile Ser Leu Phe Ser Cys Leu Lys Asp
            20                  25                  30          
Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln
        35                  40                  45              
Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe
    50                  55                  60                  
Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu
65                  70                  75                  80  
Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu
                85                  90                  95      
Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Asn
            100                 105                 110         
Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu
        115                 120                 125             
Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg
    130                 135                 140                 
Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser
145                 150                 155                 160 
Leu Arg Ser Lys Glu
                165 


<210> 5
<211> 174
<212> PRT
<213> Homo sapien

<400> 5
Thr Pro Leu Gly Pro Ala Arg Ser Leu Pro Gln Ser Phe Leu Leu Lys
 1               5                  10                  15      
Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln
            20                  25                  30          
Glu Arg Leu Cys Ala Thr His Lys Leu Cys His Pro Glu Glu Leu Val
        35                  40                  45              
Leu Leu Gly His Ser Leu Gly Ile Pro Gln Ala Pro Leu Ser Ser Cys
    50                  55                  60                  
Ser Ser Gln Ser Leu Gln Leu Thr Ser Cys Leu Asp Gln Leu His Gly
65                  70                  75                  80  
Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser
                85                  90                  95      
Pro Glu Leu Ala Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Thr Asp
            100                 105                 110         
Phe Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Gly Val Ala Pro
        115                 120                 125             
Ala Val Gln Pro Thr Gln Gly Thr Met Pro Thr Phe Thr Ser Ala Phe
    130                 135                 140                 
Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser Gln Leu Gln Arg Phe
145                 150                 155                 160 
Leu Gly Leu Ala Tyr Arg Gly Leu Arg Tyr Leu Ala Glu Pro
                165                 170                 


<210> 6
<211> 244
<212> PRT
<213> Homo sapien

<400> 6
Ala Ile Leu Ser Arg Ala Pro Trp Ser Leu Gln Ser Val Asn Pro Gly
 1               5                  10                  15      
Leu Lys Thr Asn Ser Ser Lys Glu Pro Lys Phe Thr Lys Cys Arg Ser
            20                  25                  30          
Pro Glu Arg Glu Thr Phe Ser Cys His Trp Thr Asp Glu Val His His
        35                  40                  45              
Gly Thr Lys Asn Leu Gly Pro Ile Gln Leu Phe Tyr Thr Arg Arg Asn
    50                  55                  60                  
Thr Gln Glu Trp Thr Gln Glu Trp Lys Glu Cys Pro Asp Tyr Val Ser
65                  70                  75                  80  
Ala Gly Glu Asn Ser Cys Tyr Phe Asn Ser Ser Phe Thr Ser Ile Trp
                85                  90                  95      
Ile Pro Tyr Cys Ile Lys Leu Thr Ser Asn Gly Gly Thr Val Asp Glu
            100                 105                 110         
Lys Cys Phe Ser Val Asp Glu Ile Val Gln Pro Asp Pro Pro Ile Ala
        115                 120                 125             
Leu Asn Trp Thr Leu Leu Asn Val Ser Leu Thr Gly Ile His Ala Asp
    130                 135                 140                 
Ile Gln Val Arg Trp Glu Ala Pro Arg Asn Ala Asp Ile Gln Lys Gly
145                 150                 155                 160 
Trp Met Val Leu Glu Tyr Glu Leu Gln Tyr Lys Glu Val Asn Glu Thr
                165                 170                 175     
Lys Trp Lys Met Met Asp Pro Ile Leu Thr Thr Ser Val Pro Val Tyr
            180                 185                 190         
Ser Leu Lys Val Asp Lys Glu Tyr Glu Val Arg Val Arg Ser Lys Gln
        195                 200                 205             
Arg Asn Ser Gly Asn Tyr Gly Glu Phe Ser Glu Val Leu Tyr Val Thr
    210                 215                 220                 
Leu Pro Gln Met Ser Gln Phe Thr Cys Glu Glu Asp Phe Tyr His His
225                 230                 235                 240 
His His His His
                


<210> 7
<211> 228
<212> PRT
<213> Homo sapien

<400> 7
Ile Ser Tyr Asp Ser Pro Asp Tyr Thr Asp Glu Ser Cys Thr Phe Lys
 1               5                  10                  15      
Ile Ser Leu Arg Asn Phe Arg Ser Ile Leu Ser Trp Glu Leu Lys Asn
            20                  25                  30          
His Ser Ile Val Pro Thr His Tyr Thr Leu Leu Tyr Thr Ile Met Ser
        35                  40                  45              
Lys Pro Glu Asp Leu Lys Val Val Lys Asn Cys Ala Asn Thr Thr Arg
    50                  55                  60                  
Ser Phe Cys Asp Leu Thr Asp Glu Trp Arg Ser Thr His Glu Ala Tyr
65                  70                  75                  80  
Val Thr Val Leu Glu Gly Phe Ser Gly Asn Thr Thr Leu Phe Ser Cys
                85                  90                  95      
Ser His Asn Phe Trp Leu Ala Ile Asp Met Ser Phe Glu Pro Pro Glu
            100                 105                 110         
Phe Glu Ile Val Gly Phe Thr Asn His Ile Asn Val Met Val Lys Phe
        115                 120                 125             
Pro Ser Ile Val Glu Glu Glu Leu Gln Phe Asp Leu Ser Leu Val Ile
    130                 135                 140                 
Glu Glu Gln Ser Glu Gly Ile Val Lys Lys His Lys Pro Glu Ile Lys
145                 150                 155                 160 
Gly Asn Met Ser Gly Asn Phe Thr Tyr Ile Ile Asp Lys Leu Ile Pro
                165                 170                 175     
Asn Thr Asn Tyr Cys Val Ser Val Tyr Leu Glu His Ser Asp Glu Gln
            180                 185                 190         
Ala Val Ile Lys Ser Pro Leu Lys Cys Thr Leu Leu Pro Pro Gly Gln
        195                 200                 205             
Glu Ser Glu Ser Ala Glu Ser Ala Lys His His His His His His His
    210                 215                 220                 
His His His His
225             


<210> 8
<211> 602
<212> PRT
<213> Homo sapien

<400> 8
Cys Gly His Ile Ser Val Ser Ala Pro Ile Val His Leu Gly Asp Pro
 1               5                  10                  15      
Ile Thr Ala Ser Cys Ile Ile Lys Gln Asn Cys Ser His Leu Asp Pro
            20                  25                  30          
Glu Pro Gln Ile Leu Trp Arg Leu Gly Ala Glu Leu Gln Pro Gly Gly
        35                  40                  45              
Arg Gln Gln Arg Leu Ser Asp Gly Thr Gln Glu Ser Ile Ile Thr Leu
    50                  55                  60                  
Pro His Leu Asn His Thr Gln Ala Phe Leu Ser Cys Cys Leu Asn Trp
65                  70                  75                  80  
Gly Asn Ser Leu Gln Ile Leu Asp Gln Val Glu Leu Arg Ala Gly Tyr
                85                  90                  95      
Pro Pro Ala Ile Pro His Asn Leu Ser Cys Leu Met Asn Leu Thr Thr
            100                 105                 110         
Ser Ser Leu Ile Cys Gln Trp Glu Pro Gly Pro Glu Thr His Leu Pro
        115                 120                 125             
Thr Ser Phe Thr Leu Lys Ser Phe Lys Ser Arg Gly Asn Cys Gln Thr
    130                 135                 140                 
Gln Gly Asp Ser Ile Leu Asp Cys Val Pro Lys Asp Gly Gln Ser His
145                 150                 155                 160 
Cys Cys Ile Pro Arg Lys His Leu Leu Leu Tyr Gln Asn Met Gly Ile
                165                 170                 175     
Trp Val Gln Ala Glu Asn Ala Leu Gly Thr Ser Met Ser Pro Gln Leu
            180                 185                 190         
Cys Leu Asp Pro Met Asp Val Val Lys Leu Glu Pro Pro Met Leu Arg
        195                 200                 205             
Thr Met Asp Pro Ser Pro Glu Ala Ala Pro Pro Gln Ala Gly Cys Leu
    210                 215                 220                 
Gln Leu Cys Trp Glu Pro Trp Gln Pro Gly Leu His Ile Asn Gln Lys
225                 230                 235                 240 
Cys Glu Leu Arg His Lys Pro Gln Arg Gly Glu Ala Ser Trp Ala Leu
                245                 250                 255     
Val Gly Pro Leu Pro Leu Glu Ala Leu Gln Tyr Glu Leu Cys Gly Leu
            260                 265                 270         
Leu Pro Ala Thr Ala Tyr Thr Leu Gln Ile Arg Cys Ile Arg Trp Pro
        275                 280                 285             
Leu Pro Gly His Trp Ser Asp Trp Ser Pro Ser Leu Glu Leu Arg Thr
    290                 295                 300                 
Thr Glu Arg Ala Pro Thr Val Arg Leu Asp Thr Trp Trp Arg Gln Arg
305                 310                 315                 320 
Gln Leu Asp Pro Arg Thr Val Gln Leu Phe Trp Lys Pro Val Pro Leu
                325                 330                 335     
Glu Glu Asp Ser Gly Arg Ile Gln Gly Tyr Val Val Ser Trp Arg Pro
            340                 345                 350         
Ser Gly Gln Ala Gly Ala Ile Leu Pro Leu Cys Asn Thr Thr Glu Leu
        355                 360                 365             
Ser Cys Thr Phe His Leu Pro Ser Glu Ala Gln Glu Val Ala Leu Val
    370                 375                 380                 
Ala Tyr Asn Ser Ala Gly Thr Ser Arg Pro Thr Pro Val Val Phe Ser
385                 390                 395                 400 
Glu Ser Arg Gly Pro Ala Leu Thr Arg Leu His Ala Met Ala Arg Asp
                405                 410                 415     
Pro His Ser Leu Trp Val Gly Trp Glu Pro Pro Asn Pro Trp Pro Gln
            420                 425                 430         
Gly Tyr Val Ile Glu Trp Gly Leu Gly Pro Pro Ser Ala Ser Asn Ser
        435                 440                 445             
Asn Lys Thr Trp Arg Met Glu Gln Asn Gly Arg Ala Thr Gly Phe Leu
    450                 455                 460                 
Leu Lys Glu Asn Ile Arg Pro Phe Gln Leu Tyr Glu Ile Ile Val Thr
465                 470                 475                 480 
Pro Leu Tyr Gln Asp Thr Met Gly Pro Ser Gln His Val Tyr Ala Tyr
                485                 490                 495     
Ser Gln Glu Met Ala Pro Ser His Ala Pro Glu Leu His Leu Lys His
            500                 505                 510         
Ile Gly Lys Thr Trp Ala Gln Leu Glu Trp Val Pro Glu Pro Pro Glu
        515                 520                 525             
Leu Gly Lys Ser Pro Leu Thr His Tyr Thr Ile Phe Trp Thr Asn Ala
    530                 535                 540                 
Gln Asn Gln Ser Phe Ser Ala Ile Leu Asn Ala Ser Ser Arg Gly Phe
545                 550                 555                 560 
Val Leu His Gly Leu Glu Pro Ala Ser Leu Tyr His Ile His Leu Met
                565                 570                 575     
Ala Ala Ser Gln Ala Gly Ala Thr Asn Ser Thr Val Leu Thr Leu Met
            580                 585                 590         
Thr Leu Thr Pro His His His His His His
        595                 600         

