                         SEQUENCE LISTING

<110>  Novo Nordisk A/S
 
<120>  NOVEL INSULIN DERIVATIVES AND THE MEDICAL USES HEREOF

<130>  150027WO01

<140>  to be assigned
<141>  2016-07-19

<150>  EP 15182282.2
<151>  2015-08-25

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A-chain of analogue [A8R, A22K] of human insulin of Ex. Nos. 
       15-17, 19, 23-24.


<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  ACETYLATION

<400>  1

Gly Ile Val Glu Gln Cys Cys Arg Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn Lys 
            20          


<210>  2
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A-chain of analogue [A14E, A22K] of human insulin of Ex. Nos. 2, 
       4, 8, 9, 26-27.


<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  ACETYLATION

<400>  2

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Glu Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn Lys 
            20          


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A-chain of analogue [A22K] of human insulin of Ex. Nos. 1, 3, 
       5-7, 10-14, 18, 20-22, 25, 28-46.


<220>
<221>  MOD_RES
<222>  (22)..(22)
<223>  ACETYLATION

<400>  3

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn Lys 
            20          


<210>  4
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B27E, B28E, B29R, desB30] of 
       human insulin of Ex. No. 36.

<400>  4

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Glu Glu Arg 
            20                  25                  


<210>  5
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B27E, B28R, desB29, desB30] of 
       human insulin of Ex. Nos. 18-19.

<400>  5

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Glu Arg 
            20                  25              


<210>  6
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B27E, B29P, B30R] of human 
       insulin of Ex. No. 35.

<400>  6

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Glu Pro Pro Arg 
            20                  25                  30  


<210>  7
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B27P, B28R, desB29, desB30] of 
       human insulin of Ex. Nos. 13, 16.

<400>  7

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Pro Arg 
            20                  25              


<210>  8
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B28D, B29R, desB30] of human 
       insulin of Ex. No. 34.

<400>  8

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Asp Arg 
            20                  25                  


<210>  9
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B28E, B29P, B30R] of human 
       insulin of Ex. No. 21.

<400>  9

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Glu Pro Arg 
            20                  25                  30  


<210>  10
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B28E, B29R, desB30] of human 
       insulin of Ex. Nos. 11, 23.

<400>  10

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Glu Arg 
            20                  25                  


<210>  11
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B26E, B29R, desB30] of human insulin of
       Ex. Nos. 12, 15, 41-45.

<400>  11

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Pro Arg 
            20                  25                  


<210>  12
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3Q, B26E, B29R, desB30] of human insulin of
       Ex. Nos. 39, 46.

<400>  12

Phe Val Gln Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Pro Arg 
            20                  25                  


<210>  13
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3Q, B26E, B28E, B29R, desB30] of human 
       insulin of Ex. No. 40.

<400>  13

Phe Val Gln Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Glu Thr Glu Arg 
            20                  25                  


<210>  14
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B27E, B28R, desB29, desB30] of human 
       insulin of Ex. Nos. 14, 17.

<400>  14

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Glu Arg 
            20                  25              


<210>  15
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B27E, B28E, B29P, B30R] of human 
       insulin of Ex. No. 22.

<400>  15

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Glu Glu Pro Arg 
            20                  25                  30  


<210>  16
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B27E, B28E, B29R, desB30] of human 
       insulin of Ex. Nos. 1-4, 24, 26-28, 31,

<400>  16

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Glu Glu Arg 
            20                  25                  


<210>  17
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B27P, B28E, B29R, desB30] of human 
       insulin of Ex. Nos. 5, 29-30, 38.

<400>  17

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Pro Glu Arg 
            20                  25                  


<210>  18
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B27E, B29R, desB30] of human insulin of
       Ex. Nos. 6, 25, 32.

<400>  18

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Glu Pro Arg 
            20                  25                  


<210>  19
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3Q, B27E, B28E, B29R, desB30] of human 
       insulin of Ex. No. 9.

<400>  19

Phe Val Gln Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Glu Glu Arg 
            20                  25                  


<210>  20
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B28D, B29R, desB30] of human insulin of
       Ex. Nos. 7, 10, 33, 37.

<400>  20

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Arg 
            20                  25                  


<210>  21
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3E, B28E, B29P, B30R] of human insulin of 
       Ex. No. 20.

<400>  21

Phe Val Glu Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Glu Pro Arg 
            20                  25                  30  


<210>  22
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain of analogue [B3Q, B28D, B29R, desB30] of human insulin of
       Ex. No. 8.

<400>  22

Phe Val Gln Gln His Leu Cys Gly Asp His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asp Arg 
            20                  25                  


