               SEQUENCE LISTING

<110> Chr. Hansen A/S

<120> Variants of chymosins with improved proerties

<130> P5946PC00

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 381
<212> PRT
<213> Bos


<400> 1
Met Arg Cys Leu Val Val Leu Leu Ala Val Phe Ala Leu Ser Gln Gly 
1               5                   10                  15      
Ala Glu Ile Thr Arg Ile Pro Leu Tyr Lys Gly Lys Ser Leu Arg Lys 
            20                  25                  30          
Ala Leu Lys Glu His Gly Leu Leu Glu Asp Phe Leu Gln Lys Gln Gln 
        35                  40                  45              
Tyr Gly Ile Ser Ser Lys Tyr Ser Gly Phe Gly Glu Val Ala Ser Val 
    50                  55                  60                  
Pro Leu Thr Asn Tyr Leu Asp Ser Gln Tyr Phe Gly Lys Ile Tyr Leu 
65                  70                  75                  80  
Gly Thr Pro Pro Gln Glu Phe Thr Val Leu Phe Asp Thr Gly Ser Ser 
                85                  90                  95      
Asp Phe Trp Val Pro Ser Ile Tyr Cys Lys Ser Asn Ala Cys Lys Asn 
            100                 105                 110         
His Gln Arg Phe Asp Pro Arg Lys Ser Ser Thr Phe Gln Asn Leu Gly 
        115                 120                 125             
Lys Pro Leu Ser Ile His Tyr Gly Thr Gly Ser Met Gln Gly Ile Leu 
    130                 135                 140                 
Gly Tyr Asp Thr Val Thr Val Ser Asn Ile Val Asp Ile Gln Gln Thr 
145                 150                 155                 160 
Val Gly Leu Ser Thr Gln Glu Pro Gly Asp Val Phe Thr Tyr Ala Glu 
                165                 170                 175     
Phe Asp Gly Ile Leu Gly Met Ala Tyr Pro Ser Leu Ala Ser Glu Tyr 
            180                 185                 190         
Ser Ile Pro Val Phe Asp Asn Met Met Asn Arg His Leu Val Ala Gln 
        195                 200                 205             
Asp Leu Phe Ser Val Tyr Met Asp Arg Asn Gly Gln Glu Ser Met Leu 
    210                 215                 220                 
Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr Thr Gly Ser Leu His Trp 
225                 230                 235                 240 
Val Pro Val Thr Val Gln Gln Tyr Trp Gln Phe Thr Val Asp Ser Val 
                245                 250                 255     
Thr Ile Ser Gly Val Val Val Ala Cys Glu Gly Gly Cys Gln Ala Ile 
            260                 265                 270         
Leu Asp Thr Gly Thr Ser Lys Leu Val Gly Pro Ser Ser Asp Ile Leu 
        275                 280                 285             
Asn Ile Gln Gln Ala Ile Gly Ala Thr Gln Asn Gln Tyr Gly Glu Phe 
    290                 295                 300                 
Asp Ile Asp Cys Asp Asn Leu Ser Tyr Met Pro Thr Val Val Phe Glu 
305                 310                 315                 320 
Ile Asn Gly Lys Met Tyr Pro Leu Thr Pro Ser Ala Tyr Thr Ser Gln 
                325                 330                 335     
Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln Ser Glu Asn His Ser Gln 
            340                 345                 350         
Lys Trp Ile Leu Gly Asp Val Phe Ile Arg Glu Tyr Tyr Ser Val Phe 
        355                 360                 365             
Asp Arg Ala Asn Asn Leu Val Gly Leu Ala Lys Ala Ile 
    370                 375                 380     

<210> 2
<211> 381
<212> PRT
<213> Camelus


<400> 2
Met Arg Cys Leu Val Val Leu Leu Ala Ala Leu Ala Leu Ser Gln Ala 
1               5                   10                  15      
Ser Gly Ile Thr Arg Ile Pro Leu His Lys Gly Lys Thr Leu Arg Lys 
            20                  25                  30          
Ala Leu Lys Glu Arg Gly Leu Leu Glu Asp Phe Leu Gln Arg Gln Gln 
        35                  40                  45              
Tyr Ala Val Ser Ser Lys Tyr Ser Ser Leu Gly Lys Val Ala Arg Glu 
    50                  55                  60                  
Pro Leu Thr Ser Tyr Leu Asp Ser Gln Tyr Phe Gly Lys Ile Tyr Ile 
65                  70                  75                  80  
Gly Thr Pro Pro Gln Glu Phe Thr Val Val Phe Asp Thr Gly Ser Ser 
                85                  90                  95      
Asp Leu Trp Val Pro Ser Ile Tyr Cys Lys Ser Asn Val Cys Lys Asn 
            100                 105                 110         
His His Arg Phe Asp Pro Arg Lys Ser Ser Thr Phe Arg Asn Leu Gly 
        115                 120                 125             
Lys Pro Leu Ser Ile His Tyr Gly Thr Gly Ser Met Glu Gly Phe Leu 
    130                 135                 140                 
Gly Tyr Asp Thr Val Thr Val Ser Asn Ile Val Asp Pro Asn Gln Thr 
145                 150                 155                 160 
Val Gly Leu Ser Thr Glu Gln Pro Gly Glu Val Phe Thr Tyr Ser Glu 
                165                 170                 175     
Phe Asp Gly Ile Leu Gly Leu Ala Tyr Pro Ser Leu Ala Ser Glu Tyr 
            180                 185                 190         
Ser Val Pro Val Phe Asp Asn Met Met Asp Arg His Leu Val Ala Arg 
        195                 200                 205             
Asp Leu Phe Ser Val Tyr Met Asp Arg Asn Gly Gln Gly Ser Met Leu 
    210                 215                 220                 
Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr Thr Gly Ser Leu His Trp 
225                 230                 235                 240 
Val Pro Val Thr Leu Gln Gln Tyr Trp Gln Phe Thr Val Asp Ser Val 
                245                 250                 255     
Thr Ile Asn Gly Val Ala Val Ala Cys Val Gly Gly Cys Gln Ala Ile 
            260                 265                 270         
Leu Asp Thr Gly Thr Ser Val Leu Phe Gly Pro Ser Ser Asp Ile Leu 
        275                 280                 285             
Lys Ile Gln Met Ala Ile Gly Ala Thr Glu Asn Arg Tyr Gly Glu Phe 
    290                 295                 300                 
Asp Val Asn Cys Gly Asn Leu Arg Ser Met Pro Thr Val Val Phe Glu 
305                 310                 315                 320 
Ile Asn Gly Arg Asp Tyr Pro Leu Ser Pro Ser Ala Tyr Thr Ser Lys 
                325                 330                 335     
Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln Gly Asp Asn Asn Ser Glu 
            340                 345                 350         
Leu Trp Ile Leu Gly Asp Val Phe Ile Arg Glu Tyr Tyr Ser Val Phe 
        355                 360                 365             
Asp Arg Ala Asn Asn Arg Val Gly Leu Ala Lys Ala Ile 
    370                 375                 380     

<210> 3
<211> 323
<212> PRT
<213> Bos


<400> 3
Gly Glu Val Ala Ser Val Pro Leu Thr Asn Tyr Leu Asp Ser Gln Tyr 
1               5                   10                  15      
Phe Gly Lys Ile Tyr Leu Gly Thr Pro Pro Gln Glu Phe Thr Val Leu 
            20                  25                  30          
Phe Asp Thr Gly Ser Ser Asp Phe Trp Val Pro Ser Ile Tyr Cys Lys 
        35                  40                  45              
Ser Asn Ala Cys Lys Asn His Gln Arg Phe Asp Pro Arg Lys Ser Ser 
    50                  55                  60                  
Thr Phe Gln Asn Leu Gly Lys Pro Leu Ser Ile His Tyr Gly Thr Gly 
65                  70                  75                  80  
Ser Met Gln Gly Ile Leu Gly Tyr Asp Thr Val Thr Val Ser Asn Ile 
                85                  90                  95      
Val Asp Ile Gln Gln Thr Val Gly Leu Ser Thr Gln Glu Pro Gly Asp 
            100                 105                 110         
Val Phe Thr Tyr Ala Glu Phe Asp Gly Ile Leu Gly Met Ala Tyr Pro 
        115                 120                 125             
Ser Leu Ala Ser Glu Tyr Ser Ile Pro Val Phe Asp Asn Met Met Asn 
    130                 135                 140                 
Arg His Leu Val Ala Gln Asp Leu Phe Ser Val Tyr Met Asp Arg Asn 
145                 150                 155                 160 
Gly Gln Glu Ser Met Leu Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr 
                165                 170                 175     
Thr Gly Ser Leu His Trp Val Pro Val Thr Val Gln Gln Tyr Trp Gln 
            180                 185                 190         
Phe Thr Val Asp Ser Val Thr Ile Ser Gly Val Val Val Ala Cys Glu 
        195                 200                 205             
Gly Gly Cys Gln Ala Ile Leu Asp Thr Gly Thr Ser Lys Leu Val Gly 
    210                 215                 220                 
Pro Ser Ser Asp Ile Leu Asn Ile Gln Gln Ala Ile Gly Ala Thr Gln 
225                 230                 235                 240 
Asn Gln Tyr Gly Glu Phe Asp Ile Asp Cys Asp Asn Leu Ser Tyr Met 
                245                 250                 255     
Pro Thr Val Val Phe Glu Ile Asn Gly Lys Met Tyr Pro Leu Thr Pro 
            260                 265                 270         
Ser Ala Tyr Thr Ser Gln Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln 
        275                 280                 285             
Ser Glu Asn His Ser Gln Lys Trp Ile Leu Gly Asp Val Phe Ile Arg 
    290                 295                 300                 
Glu Tyr Tyr Ser Val Phe Asp Arg Ala Asn Asn Leu Val Gly Leu Ala 
305                 310                 315                 320 
Lys Ala Ile 
            

<210> 4
<211> 323
<212> PRT
<213> Camelus


<400> 4
Gly Lys Val Ala Arg Glu Pro Leu Thr Ser Tyr Leu Asp Ser Gln Tyr 
1               5                   10                  15      
Phe Gly Lys Ile Tyr Ile Gly Thr Pro Pro Gln Glu Phe Thr Val Val 
            20                  25                  30          
Phe Asp Thr Gly Ser Ser Asp Leu Trp Val Pro Ser Ile Tyr Cys Lys 
        35                  40                  45              
Ser Asn Val Cys Lys Asn His His Arg Phe Asp Pro Arg Lys Ser Ser 
    50                  55                  60                  
Thr Phe Arg Asn Leu Gly Lys Pro Leu Ser Ile His Tyr Gly Thr Gly 
65                  70                  75                  80  
Ser Met Glu Gly Phe Leu Gly Tyr Asp Thr Val Thr Val Ser Asn Ile 
                85                  90                  95      
Val Asp Pro Asn Gln Thr Val Gly Leu Ser Thr Glu Gln Pro Gly Glu 
            100                 105                 110         
Val Phe Thr Tyr Ser Glu Phe Asp Gly Ile Leu Gly Leu Ala Tyr Pro 
        115                 120                 125             
Ser Leu Ala Ser Glu Tyr Ser Val Pro Val Phe Asp Asn Met Met Asp 
    130                 135                 140                 
Arg His Leu Val Ala Arg Asp Leu Phe Ser Val Tyr Met Asp Arg Asn 
145                 150                 155                 160 
Gly Gln Gly Ser Met Leu Thr Leu Gly Ala Ile Asp Pro Ser Tyr Tyr 
                165                 170                 175     
Thr Gly Ser Leu His Trp Val Pro Val Thr Leu Gln Gln Tyr Trp Gln 
            180                 185                 190         
Phe Thr Val Asp Ser Val Thr Ile Asn Gly Val Ala Val Ala Cys Val 
        195                 200                 205             
Gly Gly Cys Gln Ala Ile Leu Asp Thr Gly Thr Ser Val Leu Phe Gly 
    210                 215                 220                 
Pro Ser Ser Asp Ile Leu Lys Ile Gln Met Ala Ile Gly Ala Thr Glu 
225                 230                 235                 240 
Asn Arg Tyr Gly Glu Phe Asp Val Asn Cys Gly Asn Leu Arg Ser Met 
                245                 250                 255     
Pro Thr Val Val Phe Glu Ile Asn Gly Arg Asp Tyr Pro Leu Ser Pro 
            260                 265                 270         
Ser Ala Tyr Thr Ser Lys Asp Gln Gly Phe Cys Thr Ser Gly Phe Gln 
        275                 280                 285             
Gly Asp Asn Asn Ser Glu Leu Trp Ile Leu Gly Asp Val Phe Ile Arg 
    290                 295                 300                 
Glu Tyr Tyr Ser Val Phe Asp Arg Ala Asn Asn Arg Val Gly Leu Ala 
305                 310                 315                 320 
Lys Ala Ile 
            

