                         SEQUENCE LISTING

<110>  ViroMed Co., Ltd.
       bluebird bio, Inc.
       Morgan, Richard
       Friedman, Kevin
       Yu, Seung Shin
       Jeong, Jae-Gyun
       Chae, Jin-A
 
<120>  ANTI-SIALYL TN CHIMERIC ANTIGEN RECEPTORS

<130>  BLBD-066/02WO

<150>  US 62/317950
<151>  2016-04-04

<150>  KR 10-2015-0122727
<151>  2015-08-31

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  1

Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  2
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  2

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  3
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  3

Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr 
1               5                   


<210>  4
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  4

Gly Tyr Thr Phe Thr Asp His Ala Ile His 
1               5                   10  


<210>  5
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  5

Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  6

Ser Leu Asn Met Ala Tyr 
1               5       


<210>  7
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric light chain sequence

<400>  7

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  8
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric heavy chain sequence

<400>  8

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  9
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  9

Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  10
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  10

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  11

Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr 
1               5                   


<210>  12
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  12

Gly Tyr Thr Phe Thr Asp His Ala Ile His 
1               5                   10  


<210>  13
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  13

Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  14

Ser Trp Ile Met Gln Tyr 
1               5       


<210>  15
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric light chain sequence

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  16
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric heavy chain sequence

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Trp Ile Met Gln Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  17
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  17

Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  18
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  18

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  19
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  19

Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr 
1               5                   


<210>  20
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  20

Gly Tyr Thr Phe Thr Asp His Ala Ile His 
1               5                   10  


<210>  21
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  21

Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  22
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  22

Ser Trp Ile Met Gln Tyr 
1               5       


<210>  23
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric light chain sequence

<400>  23

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile 
            100                 105                 110         


Lys 
    


<210>  24
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric heavy chain sequence

<400>  24

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Trp Ile Met Gln Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  25
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized Anti-STN CAR

<400>  25

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val 
        35                  40                  45              


Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys 
        115                 120                 125             


Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Asp His Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu 
            180                 185                 190         


Glu Trp Met Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser 
        195                 200                 205             


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser 
    210                 215                 220                 


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr 
                245                 250                 255     


Leu Val Thr Val Ser Ser Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  26
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized Anti-STN CAR

<400>  26

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val 
        35                  40                  45              


Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gln Gly Thr Lys 
        115                 120                 125             


Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Asp His Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu 
            180                 185                 190         


Glu Trp Met Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser 
        195                 200                 205             


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser 
    210                 215                 220                 


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Ser Trp Ile Met Gln Tyr Trp Gly Gln Gly Thr 
                245                 250                 255     


Leu Val Thr Val Ser Ser Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  27
<211>  488
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized Anti-STN CAR

<400>  27

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val 
            20                  25                  30          


Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val 
        35                  40                  45              


Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr 
            100                 105                 110         


Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys 
        115                 120                 125             


Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
145                 150                 155                 160 


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
                165                 170                 175     


Thr Asp His Ala Ile His Trp Val Arg Gln Ala Pro Gly Gln Arg Leu 
            180                 185                 190         


Glu Trp Met Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Ser 
        195                 200                 205             


Gln Lys Phe Gln Gly Arg Val Thr Ile Thr Ala Asp Lys Ser Ala Ser 
    210                 215                 220                 


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
225                 230                 235                 240 


Tyr Tyr Cys Ala Arg Ser Trp Ile Met Gln Tyr Trp Gly Gln Gly Thr 
                245                 250                 255     


Leu Val Thr Val Ser Ser Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
305                 310                 315                 320 


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg 
                325                 330                 335     


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
            340                 345                 350         


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
        355                 360                 365             


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
    370                 375                 380                 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
385                 390                 395                 400 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
                405                 410                 415     


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
            420                 425                 430         


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
        435                 440                 445             


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
    450                 455                 460                 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
465                 470                 475                 480 


His Met Gln Ala Leu Pro Pro Arg 
                485             


<210>  28
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  28

Asp Gly Gly Gly Ser 
1               5   


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  29

Asp Gly Gly Gly Ser 
1               5   


<210>  30
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  30

Gly Gly Arg Arg 
1               


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  31

Gly Gly Gly Gly Ser 
1               5   


<210>  32
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  32

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  33
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  33

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  34

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  35
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  35

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  36

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  37
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  37

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  38
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  38

Gly Ser Thr Ser Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr 
1               5                   10                  15      


Lys Gly 
        


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  39

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  40

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  41

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  42
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  42

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  43
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  43

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  44
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  44

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  45
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  45

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  46
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  46

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  47
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  47

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  48
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  48

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  49
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  49

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  50
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  50

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  51
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  51

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  52
<211>  937
<212>  PRT
<213>  Homo sapiens

<400>  52

Met His Arg Pro Arg Arg Arg Gly Thr Arg Pro Pro Leu Leu Ala Leu 
1               5                   10                  15      


Leu Ala Ala Leu Leu Leu Ala Ala Arg Gly Ala Ala Ala Gln Glu Thr 
            20                  25                  30          


Glu Leu Ser Val Ser Ala Glu Leu Val Pro Thr Ser Ser Trp Asn Ile 
        35                  40                  45              


Ser Ser Glu Leu Asn Lys Asp Ser Tyr Leu Thr Leu Asp Glu Pro Met 
    50                  55                  60                  


Asn Asn Ile Thr Thr Ser Leu Gly Gln Thr Ala Glu Leu His Cys Lys 
65                  70                  75                  80  


Val Ser Gly Asn Pro Pro Pro Thr Ile Arg Trp Phe Lys Asn Asp Ala 
                85                  90                  95      


Pro Val Val Gln Glu Pro Arg Arg Leu Ser Phe Arg Ser Thr Ile Tyr 
            100                 105                 110         


Gly Ser Arg Leu Arg Ile Arg Asn Leu Asp Thr Thr Asp Thr Gly Tyr 
        115                 120                 125             


Phe Gln Cys Val Ala Thr Asn Gly Lys Glu Val Val Ser Ser Thr Gly 
    130                 135                 140                 


Val Leu Phe Val Lys Phe Gly Pro Pro Pro Thr Ala Ser Pro Gly Tyr 
145                 150                 155                 160 


Ser Asp Glu Tyr Glu Glu Asp Gly Phe Cys Gln Pro Tyr Arg Gly Ile 
                165                 170                 175     


Ala Cys Ala Arg Phe Ile Gly Asn Arg Thr Val Tyr Met Glu Ser Leu 
            180                 185                 190         


His Met Gln Gly Glu Ile Glu Asn Gln Ile Thr Ala Ala Phe Thr Met 
        195                 200                 205             


Ile Gly Thr Ser Ser His Leu Ser Asp Lys Cys Ser Gln Phe Ala Ile 
    210                 215                 220                 


Pro Ser Leu Cys His Tyr Ala Phe Pro Tyr Cys Asp Glu Thr Ser Ser 
225                 230                 235                 240 


Val Pro Lys Pro Arg Asp Leu Cys Arg Asp Glu Cys Glu Ile Leu Glu 
                245                 250                 255     


Asn Val Leu Cys Gln Thr Glu Tyr Ile Phe Ala Arg Ser Asn Pro Met 
            260                 265                 270         


Ile Leu Met Arg Leu Lys Leu Pro Asn Cys Glu Asp Leu Pro Gln Pro 
        275                 280                 285             


Glu Ser Pro Glu Ala Ala Asn Cys Ile Arg Ile Gly Ile Pro Met Ala 
    290                 295                 300                 


Asp Pro Ile Asn Lys Asn His Lys Cys Tyr Asn Ser Thr Gly Val Asp 
305                 310                 315                 320 


Tyr Arg Gly Thr Val Ser Val Thr Lys Ser Gly Arg Gln Cys Gln Pro 
                325                 330                 335     


Trp Asn Ser Gln Tyr Pro His Thr His Thr Phe Thr Ala Leu Arg Phe 
            340                 345                 350         


Pro Glu Leu Asn Gly Gly His Ser Tyr Cys Arg Asn Pro Gly Asn Gln 
        355                 360                 365             


Lys Glu Ala Pro Trp Cys Phe Thr Leu Asp Glu Asn Phe Lys Ser Asp 
    370                 375                 380                 


Leu Cys Asp Ile Pro Ala Cys Asp Ser Lys Asp Ser Lys Glu Lys Asn 
385                 390                 395                 400 


Lys Met Glu Ile Leu Tyr Ile Leu Val Pro Ser Val Ala Ile Pro Leu 
                405                 410                 415     


Ala Ile Ala Leu Leu Phe Phe Phe Ile Cys Val Cys Arg Asn Asn Gln 
            420                 425                 430         


Lys Ser Ser Ser Ala Pro Val Gln Arg Gln Pro Lys His Val Arg Gly 
        435                 440                 445             


Gln Asn Val Glu Met Ser Met Leu Asn Ala Tyr Lys Pro Lys Ser Lys 
    450                 455                 460                 


Ala Lys Glu Leu Pro Leu Ser Ala Val Arg Phe Met Glu Glu Leu Gly 
465                 470                 475                 480 


Glu Cys Ala Phe Gly Lys Ile Tyr Lys Gly His Leu Tyr Leu Pro Gly 
                485                 490                 495     


Met Asp His Ala Gln Leu Val Ala Ile Lys Thr Leu Lys Asp Tyr Asn 
            500                 505                 510         


Asn Pro Gln Gln Trp Thr Glu Phe Gln Gln Glu Ala Ser Leu Met Ala 
        515                 520                 525             


Glu Leu His His Pro Asn Ile Val Cys Leu Leu Gly Ala Val Thr Gln 
    530                 535                 540                 


Glu Gln Pro Val Cys Met Leu Phe Glu Tyr Ile Asn Gln Gly Asp Leu 
545                 550                 555                 560 


His Glu Phe Leu Ile Met Arg Ser Pro His Ser Asp Val Gly Cys Ser 
                565                 570                 575     


Ser Asp Glu Asp Gly Thr Val Lys Ser Ser Leu Asp His Gly Asp Phe 
            580                 585                 590         


Leu His Ile Ala Ile Gln Ile Ala Ala Gly Met Glu Tyr Leu Ser Ser 
        595                 600                 605             


His Phe Phe Val His Lys Asp Leu Ala Ala Arg Asn Ile Leu Ile Gly 
    610                 615                 620                 


Glu Gln Leu His Val Lys Ile Ser Asp Leu Gly Leu Ser Arg Glu Ile 
625                 630                 635                 640 


Tyr Ser Ala Asp Tyr Tyr Arg Val Gln Ser Lys Ser Leu Leu Pro Ile 
                645                 650                 655     


Arg Trp Met Pro Pro Glu Ala Ile Met Tyr Gly Lys Phe Ser Ser Asp 
            660                 665                 670         


Ser Asp Ile Trp Ser Phe Gly Val Val Leu Trp Glu Ile Phe Ser Phe 
        675                 680                 685             


Gly Leu Gln Pro Tyr Tyr Gly Phe Ser Asn Gln Glu Val Ile Glu Met 
    690                 695                 700                 


Val Arg Lys Arg Gln Leu Leu Pro Cys Ser Glu Asp Cys Pro Pro Arg 
705                 710                 715                 720 


Met Tyr Ser Leu Met Thr Glu Cys Trp Asn Glu Ile Pro Ser Arg Arg 
                725                 730                 735     


Pro Arg Phe Lys Asp Ile His Val Arg Leu Arg Ser Trp Glu Gly Leu 
            740                 745                 750         


Ser Ser His Thr Ser Ser Thr Thr Pro Ser Gly Gly Asn Ala Thr Thr 
        755                 760                 765             


Gln Thr Thr Ser Leu Ser Ala Ser Pro Val Ser Asn Leu Ser Asn Pro 
    770                 775                 780                 


Arg Tyr Pro Asn Tyr Met Phe Pro Ser Gln Gly Ile Thr Pro Gln Gly 
785                 790                 795                 800 


Gln Ile Ala Gly Phe Ile Gly Pro Pro Ile Pro Gln Asn Gln Arg Phe 
                805                 810                 815     


Ile Pro Ile Asn Gly Tyr Pro Ile Pro Pro Gly Tyr Ala Ala Phe Pro 
            820                 825                 830         


Ala Ala His Tyr Gln Pro Thr Gly Pro Pro Arg Val Ile Gln His Cys 
        835                 840                 845             


Pro Pro Pro Lys Ser Arg Ser Pro Ser Ser Ala Ser Gly Ser Thr Ser 
    850                 855                 860                 


Thr Gly His Val Thr Ser Leu Pro Ser Ser Gly Ser Asn Gln Glu Ala 
865                 870                 875                 880 


Asn Ile Pro Leu Leu Pro His Met Ser Ile Pro Asn His Pro Gly Gly 
                885                 890                 895     


Met Gly Ile Thr Val Phe Gly Asn Lys Ser Gln Lys Pro Tyr Lys Ile 
            900                 905                 910         


Asp Ser Lys Gln Ala Ser Leu Leu Gly Asp Ala Asn Ile His Gly His 
        915                 920                 925             


Thr Glu Ser Met Ile Ser Ala Glu Leu 
    930                 935         


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining light chain CDR-L3 motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  53

Phe Gly Xaa Gly 
1               


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H1motif


<220>
<221>  MISC_FEATURE
<222>  (2)..(4)
<223>  Xaa is any amino acid

<400>  54

Cys Xaa Xaa Xaa 
1               


<210>  55
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H2motif

<400>  55

Leu Glu Trp Ile Gly 
1               5   


<210>  56
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary rule for determining heavy chain CDR-H3 motif


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<400>  56

Trp Gly Xaa Gly 
1               


<210>  57
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  57
gccrccatgg                                                              10


