                         SEQUENCE LISTING

<110>  Qiagen GmbH
 
<120>  Polymerase Enzyme

<130>  B051-0167WO1

<150>  EP 15 18 4470.1
<151>  2015-09-09

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  2364
<212>  DNA
<213>  Thermococcus sp. AN1

<400>  1
agatctatga aacaccacca ccatcatcac attttggaca cggactacat cactgaaaac       60

ggtaagccgg ttattcgcgt gtttaagaaa gaaaatggtg agttcaagat cgagtacgac      120

cgtacctttg aaccgtactt ctatgcgctg ctgaaagacg acagcgccat cgaagatgtg      180

aagaaggtta ccgcgaaacg tcatggcacc gttgttaaag tcaagcgtgc agagaaggtg      240

cagaagaagt ttctgggtcg tccgatcgag gtgtggaaac tgtatttcaa ccatccgcag      300

gacgttcctg ccatccgtga ccgtattcgc gcacatccgg cggttgtgga catttacgaa      360

tacgacatcc ctttcgcaaa acgttatctg attgataagg gcttgattcc aatggaaggt      420

gatgaagaac tgaccatgct ggcgtttgat atcgaaaccc tgtaccacga gggcgaagag      480

ttcggtacgg gccctatttt gatgatttcc tacgccgacg gcagcgaagc gcgtgtgatt      540

acctggaaaa agattgatct gccgtatgtc gacgtcgtga gcaccgaaaa agagatgatc      600

aagcgctttc tgcgtgttgt gcgtgagaag gatccggacg tcctgattac gtacaatggt      660

gacaactttg cttttgcgta tttgaaaaag cgtagcgaag agctgggtat caagtttacc      720

ctgggtcgcg atggtagcga gccaaagatc caacgtatgg gtgaccgctt cgcggttgag      780

gttaaaggcc gtatccactt cgacctgtac ccggtcatcc gtcgcaccat caatctgccg      840

acctataccc tggaagcggt ctatgaggct gtgttcggca agccgaaaga aaaggtttat      900

gccgaagaga ttgcacaggc gtgggagagc ggcgagggtc tggagcgtgt ggcacgctat      960

agcatggaag ctgccaaagt gacctatgag ctgggccgtg aattcttccc gatggaagcg     1020

cagctgagcc gtctgattgg ccaaagcctg tgggatgtaa gccgctcttc tacgggcaac     1080

ctggtcgagt ggttcctgct gcgcaaggcg tacaaacgta atgagctggc accgaacaaa     1140

ccggatgagc gtgaactggc ccgtcgtcgt ggtggttatg cgggtggcta cgtgaaagag     1200

ccggagcgtg gcttgtggga taacattgtg tacctggact tccgcagcta tacttctagc     1260

atcatcatca cgcataacgt tagcccggac acgttgaacc gtgaaggttg taaagagtac     1320

gacgttgccc cggaagttgg tcacaagttt tgtaaagact tcccgggttt catcccgagc     1380

ctgctgggtg atctgctgga agaacgccaa aagatcaaac gcaagatgaa agcgacggtt     1440

gatccgttgg agaaaaagct gttggattac cgccaacgtc tgattaagat tctggcaaat     1500

agcttttacg gttactacgg ttatgcaaaa gcccgctggt attgcaaaga gtgcgcggag     1560

tccgtcacgg cttggggccg tgagtatatc gaaatggtta tccgcgagct ggaagagaaa     1620

ttcggcttta aggtcctgta cgcggacacc gacggcctgc acgcaaccat cccgggtgca     1680

gatgcggaga ctgttaagaa gaaggcgaaa gagtttctga aatacatcaa tccgaaattg     1740

ccgggtctgc tggagctgga atatgagggc ttttatgtcc gtggtttctt cgtgaccaaa     1800

aagaaatacg cggtcattga cgaagagggt aagattacga cccgcggttt ggagattgtc     1860

cgtcgtgact ggtcggagat cgctaaagaa acccaagcac gtgtactgga agcgattctg     1920

aaacacggtg acgtggaaga ggcagttcgc atcgttaaag aggtgacgga gaagctgagc     1980

aaatatgagg ttccgcctga aaagctggtg attcatgaac agatcacgcg cgatctgcgc     2040

gattacaaag cgaccggtcc gcacgttgcg gtcgccaagc gtctggcggc tcgcggtgtc     2100

aagattcgtc cgggcacggt tatcagctac atcgtgctga agggttccgg tcgtattggc     2160

gatcgtgcta ttccggcgga cgaattcgat ccgaccaaac accgttacga tgctgagtac     2220

tacattgaaa atcaggtgct gccggcagtt gaacgtatcc tgaaagcatt tggttatcgt     2280

aaagaggatc tgcgctacca aaagaccaaa caggtcggcc tgggcgcctg gctgaaagtg     2340

aaaggtaaga agtaatgaaa gctt                                            2364


<210>  2
<211>  782
<212>  PRT
<213>  Thermococcus sp. AN1

<400>  2

Met Lys His His His His His His Ile Leu Asp Thr Asp Tyr Ile Thr 
1               5                   10                  15      


Glu Asn Gly Lys Pro Val Ile Arg Val Phe Lys Lys Glu Asn Gly Glu 
            20                  25                  30          


Phe Lys Ile Glu Tyr Asp Arg Thr Phe Glu Pro Tyr Phe Tyr Ala Leu 
        35                  40                  45              


Leu Lys Asp Asp Ser Ala Ile Glu Asp Val Lys Lys Val Thr Ala Lys 
    50                  55                  60                  


Arg His Gly Thr Val Val Lys Val Lys Arg Ala Glu Lys Val Gln Lys 
65                  70                  75                  80  


Lys Phe Leu Gly Arg Pro Ile Glu Val Trp Lys Leu Tyr Phe Asn His 
                85                  90                  95      


Pro Gln Asp Val Pro Ala Ile Arg Asp Arg Ile Arg Ala His Pro Ala 
            100                 105                 110         


Val Val Asp Ile Tyr Glu Tyr Asp Ile Pro Phe Ala Lys Arg Tyr Leu 
        115                 120                 125             


Ile Asp Lys Gly Leu Ile Pro Met Glu Gly Asp Glu Glu Leu Thr Met 
    130                 135                 140                 


Leu Ala Phe Asp Ile Glu Thr Leu Tyr His Glu Gly Glu Glu Phe Gly 
145                 150                 155                 160 


Thr Gly Pro Ile Leu Met Ile Ser Tyr Ala Asp Gly Ser Glu Ala Arg 
                165                 170                 175     


Val Ile Thr Trp Lys Lys Ile Asp Leu Pro Tyr Val Asp Val Val Ser 
            180                 185                 190         


Thr Glu Lys Glu Met Ile Lys Arg Phe Leu Arg Val Val Arg Glu Lys 
        195                 200                 205             


Asp Pro Asp Val Leu Ile Thr Tyr Asn Gly Asp Asn Phe Ala Phe Ala 
    210                 215                 220                 


Tyr Leu Lys Lys Arg Ser Glu Glu Leu Gly Ile Lys Phe Thr Leu Gly 
225                 230                 235                 240 


Arg Asp Gly Ser Glu Pro Lys Ile Gln Arg Met Gly Asp Arg Phe Ala 
                245                 250                 255     


Val Glu Val Lys Gly Arg Ile His Phe Asp Leu Tyr Pro Val Ile Arg 
            260                 265                 270         


Arg Thr Ile Asn Leu Pro Thr Tyr Thr Leu Glu Ala Val Tyr Glu Ala 
        275                 280                 285             


Val Phe Gly Lys Pro Lys Glu Lys Val Tyr Ala Glu Glu Ile Ala Gln 
    290                 295                 300                 


Ala Trp Glu Ser Gly Glu Gly Leu Glu Arg Val Ala Arg Tyr Ser Met 
305                 310                 315                 320 


Glu Ala Ala Lys Val Thr Tyr Glu Leu Gly Arg Glu Phe Phe Pro Met 
                325                 330                 335     


Glu Ala Gln Leu Ser Arg Leu Ile Gly Gln Ser Leu Trp Asp Val Ser 
            340                 345                 350         


Arg Ser Ser Thr Gly Asn Leu Val Glu Trp Phe Leu Leu Arg Lys Ala 
        355                 360                 365             


Tyr Lys Arg Asn Glu Leu Ala Pro Asn Lys Pro Asp Glu Arg Glu Leu 
    370                 375                 380                 


Ala Arg Arg Arg Gly Gly Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu 
385                 390                 395                 400 


Arg Gly Leu Trp Asp Asn Ile Val Tyr Leu Asp Phe Arg Ser Tyr Thr 
                405                 410                 415     


Ser Ser Ile Ile Ile Thr His Asn Val Ser Pro Asp Thr Leu Asn Arg 
            420                 425                 430         


Glu Gly Cys Lys Glu Tyr Asp Val Ala Pro Glu Val Gly His Lys Phe 
        435                 440                 445             


Cys Lys Asp Phe Pro Gly Phe Ile Pro Ser Leu Leu Gly Asp Leu Leu 
    450                 455                 460                 


Glu Glu Arg Gln Lys Ile Lys Arg Lys Met Lys Ala Thr Val Asp Pro 
465                 470                 475                 480 


Leu Glu Lys Lys Leu Leu Asp Tyr Arg Gln Arg Leu Ile Lys Ile Leu 
                485                 490                 495     


Ala Asn Ser Phe Tyr Gly Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr 
            500                 505                 510         


Cys Lys Glu Cys Ala Glu Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile 
        515                 520                 525             


Glu Met Val Ile Arg Glu Leu Glu Glu Lys Phe Gly Phe Lys Val Leu 
    530                 535                 540                 


Tyr Ala Asp Thr Asp Gly Leu His Ala Thr Ile Pro Gly Ala Asp Ala 
545                 550                 555                 560 


Glu Thr Val Lys Lys Lys Ala Lys Glu Phe Leu Lys Tyr Ile Asn Pro 
                565                 570                 575     


Lys Leu Pro Gly Leu Leu Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg 
            580                 585                 590         


Gly Phe Phe Val Thr Lys Lys Lys Tyr Ala Val Ile Asp Glu Glu Gly 
        595                 600                 605             


Lys Ile Thr Thr Arg Gly Leu Glu Ile Val Arg Arg Asp Trp Ser Glu 
    610                 615                 620                 


Ile Ala Lys Glu Thr Gln Ala Arg Val Leu Glu Ala Ile Leu Lys His 
625                 630                 635                 640 


Gly Asp Val Glu Glu Ala Val Arg Ile Val Lys Glu Val Thr Glu Lys 
                645                 650                 655     


Leu Ser Lys Tyr Glu Val Pro Pro Glu Lys Leu Val Ile His Glu Gln 
            660                 665                 670         


Ile Thr Arg Asp Leu Arg Asp Tyr Lys Ala Thr Gly Pro His Val Ala 
        675                 680                 685             


Val Ala Lys Arg Leu Ala Ala Arg Gly Val Lys Ile Arg Pro Gly Thr 
    690                 695                 700                 


Val Ile Ser Tyr Ile Val Leu Lys Gly Ser Gly Arg Ile Gly Asp Arg 
705                 710                 715                 720 


Ala Ile Pro Ala Asp Glu Phe Asp Pro Thr Lys His Arg Tyr Asp Ala 
                725                 730                 735     


Glu Tyr Tyr Ile Glu Asn Gln Val Leu Pro Ala Val Glu Arg Ile Leu 
            740                 745                 750         


Lys Ala Phe Gly Tyr Arg Lys Glu Asp Leu Arg Tyr Gln Lys Thr Lys 
        755                 760                 765             


Gln Val Gly Leu Gly Ala Trp Leu Lys Val Lys Gly Lys Lys 
    770                 775                 780         


<210>  3
<211>  2364
<212>  DNA
<213>  Thermococcus sp. AN1

<400>  3
agatctatga aacaccacca ccatcatcac attttggaca cggactacat cactgaaaac       60

ggtaagccgg ttattcgcgt gtttaagaaa gaaaatggtg agttcaagat cgagtacgac      120

cgtacctttg aaccgtactt ctatgcgctg ctgaaagacg acagcgccat cgaagatgtg      180

aagaaggtta ccgcgaaacg tcatggcacc gttgttaaag tcaagcgtgc agagaaggtg      240

cagaagaagt ttctgggtcg tccgatcgag gtgtggaaac tgtatttcaa ccatccgcag      300

gacgttcctg ccatccgtga ccgtattcgc gcacatccgg cggttgtgga catttacgaa      360

tacgacatcc ctttcgcaaa acgttatctg attgataagg gcttgattcc aatggaaggt      420

gatgaagaac tgaccatgct ggcgtttgat atcgaaaccc tgtaccacga gggcgaagag      480

ttcggtacgg gccctatttt gatgatttcc tacgccgacg gcagcgaagc gcgtgtgatt      540

acctggaaaa agattgatct gccgtatgtc gacgtcgtga gcaccgaaaa agagatgatc      600

aagcgctttc tgcgtgttgt gcgtgagaag gatccggacg tcctgattac gtacaatggt      660

gacaactttg cttttgcgta tttgaaaaag cgtagcgaag agctgggtat caagtttacc      720

ctgggtcgcg atggtagcga gccaaagatc caacgtatgg gtgaccgctt cgcggttgag      780

gttaaaggcc gtatccactt cgacctgtac ccggtcatcc gtcgcaccat caatctgccg      840

acctataccc tggaagcggt ctatgaggct gtgttcggca agccgaaaga aaaggtttat      900

gccgaagaga ttgcacaggc gtgggagagc ggcgagggtc tggagcgtgt ggcacgctat      960

agcatggaag ctgccaaagt gacctatgag ctgggccgtg aattcttccc gatggaagcg     1020

cagctgagcc gtctgattgg ccaaagcctg tgggatgtaa gccgctcttc tacgggcaac     1080

ctggtcgagt ggttcctgct gcgcaaggcg tacaaacgta atgagctggc accgaacaaa     1140

ccggatgagc gtgaactggc ccgtcgtcgt ggtggttatg cgggtggcta cgtgaaagag     1200

ccggagcgtg gcttgtggga taacattgtg tacctggact tccgcagctt tactgggagc     1260

atcatcatca cgcataacgt tagcccggac acgttgaacc gtgaaggttg taaagagtac     1320

gacgttgccc cggaagttgg tcacaagttt tgtaaagact tcccgggttt catcccgagc     1380

ctgctgggtg atctgctgga agaacgccaa aagatcaaac gcaagatgaa agcgacggtt     1440

gatccgttgg agaaaaagct gttggattac cgccaacgtc tgattaagat tctggcaaat     1500

agcttttacg gttactacgg ttatgcaaaa gcccgctggt attgcaaaga gtgcgcggag     1560

tccgtcacgg cttggggccg tgagtatatc gaaatggtta tccgcgagct ggaagagaaa     1620

ttcggcttta aggtcctgta cgcggacacc gacggcctgc acgcaaccat cccgggtgca     1680

gatgcggaga ctgttaagaa gaaggcgaaa gagtttctga aatacatcaa tccgaaattg     1740

ccgggtctgc tggagctgga atatgagggc ttttatgtcc gtggtttctt cgtgaccaaa     1800

aagaaatacg cggtcattga cgaagagggt aagattacga cccgcggttt ggagattgtc     1860

cgtcgtgact ggtcggagat cgctaaagaa acccaagcac gtgtactgga agcgattctg     1920

aaacacggtg acgtggaaga ggcagttcgc atcgttaaag aggtgacgga gaagctgagc     1980

aaatatgagg ttccgcctga aaagctggtg attcatgaac agatcacgcg cgatctgcgc     2040

gattacaaag cgaccggtcc gcacgttgcg gtcgccaagc gtctggcggc tcgcggtgtc     2100

aagattcgtc cgggcacggt tatcagctac atcgtgctga agggttccgg tcgtattggc     2160

gatcgtgcta ttccggcgga cgaattcgat ccgaccaaac accgttacga tgctgagtac     2220

tacattgaaa atcaggtgct gccggcagtt gaacgtatcc tgaaagcatt tggttatcgt     2280

aaagaggatc tgcgctacca aaagaccaaa caggtcggcc tgggcgcctg gctgaaagtg     2340

aaaggtaaga agtaatgaaa gctt                                            2364


<210>  4
<211>  782
<212>  PRT
<213>  Thermococcus sp. AN1

<400>  4

Met Lys His His His His His His Ile Leu Asp Thr Asp Tyr Ile Thr 
1               5                   10                  15      


Glu Asn Gly Lys Pro Val Ile Arg Val Phe Lys Lys Glu Asn Gly Glu 
            20                  25                  30          


Phe Lys Ile Glu Tyr Asp Arg Thr Phe Glu Pro Tyr Phe Tyr Ala Leu 
        35                  40                  45              


Leu Lys Asp Asp Ser Ala Ile Glu Asp Val Lys Lys Val Thr Ala Lys 
    50                  55                  60                  


Arg His Gly Thr Val Val Lys Val Lys Arg Ala Glu Lys Val Gln Lys 
65                  70                  75                  80  


Lys Phe Leu Gly Arg Pro Ile Glu Val Trp Lys Leu Tyr Phe Asn His 
                85                  90                  95      


Pro Gln Asp Val Pro Ala Ile Arg Asp Arg Ile Arg Ala His Pro Ala 
            100                 105                 110         


Val Val Asp Ile Tyr Glu Tyr Asp Ile Pro Phe Ala Lys Arg Tyr Leu 
        115                 120                 125             


Ile Asp Lys Gly Leu Ile Pro Met Glu Gly Asp Glu Glu Leu Thr Met 
    130                 135                 140                 


Leu Ala Phe Asp Ile Glu Thr Leu Tyr His Glu Gly Glu Glu Phe Gly 
145                 150                 155                 160 


Thr Gly Pro Ile Leu Met Ile Ser Tyr Ala Asp Gly Ser Glu Ala Arg 
                165                 170                 175     


Val Ile Thr Trp Lys Lys Ile Asp Leu Pro Tyr Val Asp Val Val Ser 
            180                 185                 190         


Thr Glu Lys Glu Met Ile Lys Arg Phe Leu Arg Val Val Arg Glu Lys 
        195                 200                 205             


Asp Pro Asp Val Leu Ile Thr Tyr Asn Gly Asp Asn Phe Ala Phe Ala 
    210                 215                 220                 


Tyr Leu Lys Lys Arg Ser Glu Glu Leu Gly Ile Lys Phe Thr Leu Gly 
225                 230                 235                 240 


Arg Asp Gly Ser Glu Pro Lys Ile Gln Arg Met Gly Asp Arg Phe Ala 
                245                 250                 255     


Val Glu Val Lys Gly Arg Ile His Phe Asp Leu Tyr Pro Val Ile Arg 
            260                 265                 270         


Arg Thr Ile Asn Leu Pro Thr Tyr Thr Leu Glu Ala Val Tyr Glu Ala 
        275                 280                 285             


Val Phe Gly Lys Pro Lys Glu Lys Val Tyr Ala Glu Glu Ile Ala Gln 
    290                 295                 300                 


Ala Trp Glu Ser Gly Glu Gly Leu Glu Arg Val Ala Arg Tyr Ser Met 
305                 310                 315                 320 


Glu Ala Ala Lys Val Thr Tyr Glu Leu Gly Arg Glu Phe Phe Pro Met 
                325                 330                 335     


Glu Ala Gln Leu Ser Arg Leu Ile Gly Gln Ser Leu Trp Asp Val Ser 
            340                 345                 350         


Arg Ser Ser Thr Gly Asn Leu Val Glu Trp Phe Leu Leu Arg Lys Ala 
        355                 360                 365             


Tyr Lys Arg Asn Glu Leu Ala Pro Asn Lys Pro Asp Glu Arg Glu Leu 
    370                 375                 380                 


Ala Arg Arg Arg Gly Gly Tyr Ala Gly Gly Tyr Val Lys Glu Pro Glu 
385                 390                 395                 400 


Arg Gly Leu Trp Asp Asn Ile Val Tyr Leu Asp Phe Arg Ser Phe Thr 
                405                 410                 415     


Gly Ser Ile Ile Ile Thr His Asn Val Ser Pro Asp Thr Leu Asn Arg 
            420                 425                 430         


Glu Gly Cys Lys Glu Tyr Asp Val Ala Pro Glu Val Gly His Lys Phe 
        435                 440                 445             


Cys Lys Asp Phe Pro Gly Phe Ile Pro Ser Leu Leu Gly Asp Leu Leu 
    450                 455                 460                 


Glu Glu Arg Gln Lys Ile Lys Arg Lys Met Lys Ala Thr Val Asp Pro 
465                 470                 475                 480 


Leu Glu Lys Lys Leu Leu Asp Tyr Arg Gln Arg Leu Ile Lys Ile Leu 
                485                 490                 495     


Ala Asn Ser Phe Tyr Gly Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr 
            500                 505                 510         


Cys Lys Glu Cys Ala Glu Ser Val Thr Ala Trp Gly Arg Glu Tyr Ile 
        515                 520                 525             


Glu Met Val Ile Arg Glu Leu Glu Glu Lys Phe Gly Phe Lys Val Leu 
    530                 535                 540                 


Tyr Ala Asp Thr Asp Gly Leu His Ala Thr Ile Pro Gly Ala Asp Ala 
545                 550                 555                 560 


Glu Thr Val Lys Lys Lys Ala Lys Glu Phe Leu Lys Tyr Ile Asn Pro 
                565                 570                 575     


Lys Leu Pro Gly Leu Leu Glu Leu Glu Tyr Glu Gly Phe Tyr Val Arg 
            580                 585                 590         


Gly Phe Phe Val Thr Lys Lys Lys Tyr Ala Val Ile Asp Glu Glu Gly 
        595                 600                 605             


Lys Ile Thr Thr Arg Gly Leu Glu Ile Val Arg Arg Asp Trp Ser Glu 
    610                 615                 620                 


Ile Ala Lys Glu Thr Gln Ala Arg Val Leu Glu Ala Ile Leu Lys His 
625                 630                 635                 640 


Gly Asp Val Glu Glu Ala Val Arg Ile Val Lys Glu Val Thr Glu Lys 
                645                 650                 655     


Leu Ser Lys Tyr Glu Val Pro Pro Glu Lys Leu Val Ile His Glu Gln 
            660                 665                 670         


Ile Thr Arg Asp Leu Arg Asp Tyr Lys Ala Thr Gly Pro His Val Ala 
        675                 680                 685             


Val Ala Lys Arg Leu Ala Ala Arg Gly Val Lys Ile Arg Pro Gly Thr 
    690                 695                 700                 


Val Ile Ser Tyr Ile Val Leu Lys Gly Ser Gly Arg Ile Gly Asp Arg 
705                 710                 715                 720 


Ala Ile Pro Ala Asp Glu Phe Asp Pro Thr Lys His Arg Tyr Asp Ala 
                725                 730                 735     


Glu Tyr Tyr Ile Glu Asn Gln Val Leu Pro Ala Val Glu Arg Ile Leu 
            740                 745                 750         


Lys Ala Phe Gly Tyr Arg Lys Glu Asp Leu Arg Tyr Gln Lys Thr Lys 
        755                 760                 765             


Gln Val Gly Leu Gly Ala Trp Leu Lys Val Lys Gly Lys Lys 
    770                 775                 780         


