SEH00122WO - Sequence Listing 30092016 
SEQUENCE LISTING 

<110> The Bio Nano Centre Limited 
<120> Dual Mediator Biosensor 
<130> SEH00122WO 
<150> GB 1517275.2 
<151> 2015-09-30 
<160> 8 
<170> PatentIn version 3.3 
<210> 1 
<211> 175 
<212> PRT 
<213> Rhizobium sp. NT-26 
<400> 1 
Met Ser Arg Cys Gln Asn Met Val Asp Ile Gly Arg Arg Gln Phe Leu 
1 5 10 15 

Arg Gly Gly Ala Leu Ala Ala Ala Gly Ala Thr Ala Ala Val Phe Gly 
20 25 30 

Val Gly Ala Pro Gln Ala Arg Ala Ala Thr Ala Ala Ala Gly Val Glu 
35 40 45 

Tyr Pro Ala Asn Arg Leu Ala Asn Ile Ser Glu Leu Thr Leu Asn Glu 
50 55 60 

Pro Leu Asp Val Ala Tyr Pro Asp Glu Asp Ala Ala Gly Val Leu Leu 
65 70 75 80 

Lys Leu Gly Thr Arg Val Glu Gly Gly Val Gly Pro Asp Gly Asp Ile 
85 90 95 

Val Gly Phe Ser Thr Ile Cys Pro His Lys Gly Phe Pro Leu Ser Tyr 
100 105 110 

Ser Ala Asp Asn Lys Thr Phe Asn Cys Pro Gly His Phe Ser Val Phe 
115 120 125 

Asp Pro Glu Lys Gly Gly Gln Gln Val Trp Gly Gln Ala Thr Gln Asn 
130 135 140 

Leu Pro Gln Tyr Val Leu Arg Val Ala Asp Asn Gly Asp Ile Phe Ala 
145 150 155 160 

Glu Gly Val Asp Glu Leu Ile Tyr Gly Arg Leu Ser Asn Val Leu 
165 170 175 

<210> 2 
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SEH00122WO - Sequence Listing 30092016 

<211> 40 
<212> PRT 
<213> Rhizobium sp. NT-26 
<400> 2 
Met Ser Arg Cys Gln Asn Met Val Asp Ile Gly Arg Arg Gln Phe Leu 
1 5 10 15 

Arg Gly Gly Ala Leu Ala Ala Ala Gly Ala Thr Ala Ala Val Phe Gly 
20 25 30 

Val Gly Ala Pro Gln Ala Arg Ala 
35 40 

<210> 3 
<211> 150 
<212> PRT 
<213> Rhizobium sp. NT-26 
<400> 3 
Ala Thr Ala Ala Val Phe Gly Val Gly Ala Pro Gln Ala Arg Ala Ala 
1 5 10 15 

Thr Ala Ala Ala Gly Val Glu Tyr Pro Ala Asn Arg Leu Ala Asn Ile 
20 25 30 

Ser Glu Leu Thr Leu Asn Glu Pro Leu Asp Val Ala Tyr Pro Asp Glu 
35 40 45 

Asp Ala Ala Gly Val Leu Leu Lys Leu Gly Thr Arg Val Glu Gly Gly 
50 55 60 

Val Gly Pro Asp Gly Asp Ile Val Gly Phe Ser Thr Ile Cys Pro His 
65 70 75 80 

Lys Gly Phe Pro Leu Ser Tyr Ser Ala Asp Asn Lys Thr Phe Asn Cys 
85 90 95 

Pro Gly His Phe Ser Val Phe Asp Pro Glu Lys Gly Gly Gln Gln Val 
100 105 110 

Trp Gly Gln Ala Thr Gln Asn Leu Pro Gln Tyr Val Leu Arg Val Ala 
115 120 125 

Asp Asn Gly Asp Ile Phe Ala Glu Gly Val Asp Glu Leu Ile Tyr Gly 
130 135 140 

Arg Leu Ser Asn Val Leu 
145 150 

<210> 4 
<211> 845 
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SEH00122WO - Sequence Listing 30092016 

<212> PRT 
<213> Rhizobium sp. NT-26 
<400> 4 
Met Ala Phe Lys Arg His Ile Asp Arg Leu Pro Ile Ile Pro Ala Asp 
1 5 10 15 

Ala Lys Lys His Asn Val Thr Cys His Phe Cys Ile Val Gly Cys Gly 
20 25 30 

Tyr His Ala Tyr Thr Trp Pro Ile Asn Lys Gln Gly Gly Thr Asp Pro 
35 40 45 

Gln Asn Asn Ile Phe Gly Val Asp Leu Ser Glu Gln Gln Gln Ala Glu 
50 55 60 

Ser Asp Ala Trp Tyr Ser Pro Ser Met Tyr Asn Val Val Lys Gln Asp 
65 70 75 80 

Gly Arg Asp Val His Val Val Ile Lys Pro Asp His Glu Cys Val Val 
85 90 95 

Asn Ser Gly Leu Gly Ser Val Arg Gly Ala Arg Met Ala Glu Thr Ser 
100 105 110 

Phe Ser Glu Ala Arg Asn Thr Gln Gln Gln Arg Leu Thr Asp Pro Leu 
115 120 125 

Val Trp Arg Tyr Gly Gln Met Gln Pro Thr Ser Trp Asp Asp Ala Leu 
130 135 140 

Asp Leu Val Ala Arg Val Thr Ala Lys Ile Val Lys Glu Lys Gly Glu 
145 150 155 160 

Asp Ala Leu Ile Val Ser Ala Phe Asp His Gly Gly Ala Gly Gly Gly 
165 170 175 

Tyr Glu Asn Thr Trp Gly Thr Gly Lys Leu Tyr Phe Glu Ala Met Lys 
180 185 190 

Val Lys Asn Ile Arg Ile His Asn Arg Pro Ala Tyr Asn Ser Glu Val 
195 200 205 

His Gly Thr Arg Asp Met Gly Val Gly Glu Leu Asn Asn Cys Tyr Glu 
210 215 220 

Asp Ala Glu Leu Ala Asp Thr Ile Val Ala Val Gly Thr Asn Ala Leu 
225 230 235 240 

Glu Thr Gln Thr Asn Tyr Phe Leu Asn His Trp Ile Pro Asn Leu Arg 
245 250 255 
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SEH00122WO - Sequence Listing 30092016 

Gly Glu Ser Leu Gly Lys Lys Lys Glu Leu Met Pro Glu Glu Pro His 
260 265 270 

Glu Ala Gly Arg Ile Ile Ile Val Asp Pro Arg Arg Thr Val Thr Val 
275 280 285 

Asn Ala Cys Glu Gln Thr Ala Gly Ala Asp Asn Val Leu His Leu Ala 
290 295 300 

Ile Asn Ser Gly Thr Asp Leu Ala Leu Phe Asn Ala Leu Phe Thr Tyr 
305 310 315 320 

Ile Ala Asp Lys Gly Trp Val Asp Arg Asp Phe Ile Asp Lys Ser Thr 
325 330 335 

Leu Arg Glu Gly Thr Ala Arg Pro Pro Leu Tyr Pro Ala Arg Gly Val 
340 345 350 

Ser Glu Ala Asn Pro Gly His Leu Ser Ser Phe Glu Asp Ala Val Glu 
355 360 365 

Gly Cys Arg Met Ser Ile Glu Glu Ala Ala Glu Ile Thr Gly Leu Asp 
370 375 380 

Ala Ala Gln Ile Ile Lys Ala Ala Glu Trp Ile Gly Met Pro Lys Glu 
385 390 395 400 

Gly Gly Lys Arg Arg Arg Val Met Phe Gly Tyr Glu Lys Gly Leu Ile 
405 410 415 

Trp Gly Asn Asp Asn Tyr Arg Thr Asn Gly Ala Leu Val Asn Leu Ala 
420 425 430 

Leu Ala Thr Gly Asn Ile Gly Arg Pro Gly Gly Gly Val Val Arg Leu 
435 440 445 

Gly Gly His Gln Glu Gly Tyr Val Arg Pro Ser Asp Ala His Val Gly 
450 455 460 

Arg Pro Ala Ala Tyr Val Asp Gln Leu Leu Ile Gly Gly Gln Gly Gly 
465 470 475 480 

Val His His Ile Trp Gly Cys Asp His Tyr Lys Thr Thr Leu Asn Ala 
485 490 495 

His Glu Phe Lys Arg Val Tyr Lys Lys Arg Thr Asp Met Val Lys Asp 
500 505 510 

Ala Met Ser Ala Ala Pro Tyr Gly Asp Arg Glu Ala Met Val Asn Ala 
515 520 525 
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SEH00122WO - Sequence Listing 30092016 

Ile Val Asp Ala Ile Asn Gln Gly Gly Leu Phe Ala Val Asn Val Asp 
530 535 540 

Ile Ile Pro Thr Lys Ile Gly Glu Ala Cys His Val Ile Leu Pro Ala 
545 550 555 560 

Ala Thr Ser Gly Glu Met Asn Leu Thr Ser Met Asn Gly Glu Arg Arg 
565 570 575 

Met Arg Leu Thr Glu Arg Tyr Met Asp Pro Pro Gly Gln Ser Met Pro 
580 585 590 

Asp Cys Leu Ile Ala Ala Arg Leu Ala Asn Thr Met Glu Arg Val Leu 
595 600 605 

Thr Glu Met Gly Asp Val Gly Tyr Ala Ala Gln Phe Lys Gly Phe Asp 
610 615 620 

Trp Gln Thr Glu Glu Asp Ala Phe Met Asp Gly Tyr Asn Lys Asn Ala 
625 630 635 640 

His Gly Gly Glu Phe Val Thr Tyr Glu Arg Leu Ser Ala Met Gly Thr 
645 650 655 

Asn Gly Phe Gln Glu Pro Ala Thr Gly Phe Thr Asp Gly Lys Ile Glu 
660 665 670 

Gly Thr Gln Arg Leu Tyr Thr Asp Gly Val Phe Ser Thr Asp Asp Gly 
675 680 685 

Lys Ala Arg Phe Met Asp Ala Pro Trp Arg Gly Leu Gln Ala Pro Gly 
690 695 700 

Lys Gln Gln Gln Lys Asp Ser His Lys Tyr Leu Ile Asn Asn Gly Arg 
705 710 715 720 

Ala Asn Val Val Trp Gln Ser Ala Tyr Leu Asp Gln Glu Asn Asp Phe 
725 730 735 

Val Met Asp Arg Phe Pro Tyr Pro Phe Ile Glu Met Asn Pro Glu Asp 
740 745 750 

Met Ala Glu Ala Gly Leu Lys Glu Gly Asp Leu Val Glu Ile Tyr Asn 
755 760 765 

Asp Ala Gly Ala Thr Gln Ala Met Ala Tyr Pro Thr Pro Thr Ala Arg 
770 775 780 

Arg Gly Glu Thr Phe Met Leu Phe Gly Phe Pro Thr Gly Val Gln Gly 
785 790 795 800 

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SEH00122WO - Sequence Listing 30092016 

Asn Val Thr Ser Ala Gly Thr Asn Glu Leu Ile Ile Pro Asn Tyr Lys 
805 810 815 

Gln Thr Trp Gly Asn Ile Arg Lys Ile Ser Asp Ala Pro Arg Asn Val 
820 825 830 

Ala His Leu Ser Phe Lys Ser Lys Glu Tyr Gln Ser Ala 
835 840 845 

<210> 5 

<211> 528 

<212> DNA 

<213> Rhizobium sp. NT-26 

<400> 5 
atgtcacgtt gtcaaaacat ggtcgatatt ggccgacgcc agttcctgcg tggaggcgcg 60 
ctcgcggctg cgggtgcgac tgccgccgtc ttcggcgtcg gcgcaccaca ggctagagcc 120 
gctaccgcgg cggcaggggt cgaatatcct gccaatcgtc tggcaaacat ctcagaactt 180 
acgctcaatg aaccgctcga tgtcgcctat ccggacgagg atgccgcagg cgttctgctt 240 
aagcttggga cccgcgtcga gggtggcgtt ggccctgacg gcgacattgt cggcttttcc 300 
acgatctgtc ctcacaaggg ttttcctctg agctactccg ccgacaacaa gacgttcaac 360 
tgtcctggtc acttctcggt cttcgaccct gaaaagggcg gccagcaggt ttggggtcag 420 
gccacgcaga acctgccgca atacgtgctc cgcgtcgccg acaatggcga catctttgcc 480 
gaaggcgtcg acgagctgat ctacggccgt ctgtccaacg ttctataa 528 

<210> 6 

<211> 120 

<212> DNA 

<213> Rhizobium sp. NT-26 

<400> 6 
atgtcacgtt gtcaaaacat ggtcgatatt ggccgacgcc agttcctgcg tggaggcgcg 60 

ctcgcggctg cgggtgcgac tgccgccgtc ttcggcgtcg gcgcaccaca ggctagagcc 120 

<210> 7 

<211> 408 

<212> DNA 

<213> Rhizobium sp. NT-26 

<400> 7 
gctaccgcgg cggcaggggt cgaatatcct gccaatcgtc tggcaaacat ctcagaactt 60 
acgctcaatg aaccgctcga tgtcgcctat ccggacgagg atgccgcagg cgttctgctt 120 
aagcttggga cccgcgtcga gggtggcgtt ggccctgacg gcgacattgt cggcttttcc 180 
acgatctgtc ctcacaaggg ttttcctctg agctactccg ccgacaacaa gacgttcaac 240 
tgtcctggtc acttctcggt cttcgaccct gaaaagggcg gccagcaggt ttggggtcag 300 
gccacgcaga acctgccgca atacgtgctc cgcgtcgccg acaatggcga catctttgcc 360 

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SEH00122WO - Sequence Listing 30092016 
gaaggcgtcg acgagctgat ctacggccgt ctgtccaacg ttctataa 408 

<210> 8 

<211> 2538 

<212> DNA 

<213> Rhizobium sp. NT-26 

<400> 8 
atggccttca aacgtcacat cgaccgtctg ccgatcattc ccgcggacgc caagaagcac 60 
aatgtcacct gccacttctg catcgtcggt tgcggctatc acgcctatac ctggccgatc 120 
aacaaacaag gcggtacgga tccacagaac aacatcttcg gcgtcgacct gtctgaacag 180 
cagcaggcgg aaagcgacgc ctggtattca ccgtccatgt acaacgtggt caagcaggat 240 
ggccgcgacg ttcatgtcgt catcaagcca gaccacgaat gtgtcgtgaa ctccggtctc 300 
ggttcggtgc gtggcgcccg catggcagag acgagcttct cagaggcccg caacacccag 360 
cagcagcgcc tcaccgatcc gcttgtctgg cgatacgggc agatgcaacc gacgagctgg 420 
gacgacgcgc tcgatctcgt cgctcgcgtg accgcgaaga tcgtcaaaga gaagggtgag 480 
gacgccctca tcgtatcggc ctttgaccat ggcggtgcag gcggcggcta cgagaacacc 540 
tggggcacgg gaaagctcta tttcgaggcc atgaaggtca agaacatccg catccacaac 600 
cgcccggcct acaattccga ggttcacggc acccgcgaca tgggcgtcgg cgagttgaat 660 
aactgctacg aggatgccga actggccgac acgatcgttg cggttggcac caacgcgctg 720 
gagacccaga ccaactactt cctaaatcac tggattccga atctgcgcgg cgaaagcctc 780 
ggcaagaaaa aggagctcat gccggaggag ccccatgaag caggcaggat cattatcgtc 840 
gatccgcgcc gcaccgtgac ggtcaatgcc tgcgagcaga cggccggcgc cgacaatgtc 900 
ctgcatcttg ctatcaattc tggcacggac ctcgcccttt tcaacgcact cttcacctat 960 
atcgccgaca agggctgggt cgatcgcgac ttcatcgaca agtcgacact gcgcgagggt 1020 
acagcccgac cgccgctcta tcctgcccgt ggagtgtcag aggccaatcc ggggcatctc 1080 
tcgagtttcg aggacgccgt ggaaggctgc cgcatgtcta tcgaggaggc tgcggaaatc 1140 
accggtctcg acgccgccca gatcatcaag gcagccgagt ggatcggcat gcccaaggaa 1200 
ggcggcaagc gccgccgtgt catgttcggt tacgagaagg gtctgatctg gggcaatgac 1260 
aactaccgaa ccaacggcgc gctggtgaac ctcgcccttg ccaccggcaa tatcggccgt 1320 
cccggtggcg gcgtcgtacg ccttggcgga caccaggaag gctatgtgcg cccctccgac 1380 
gcccatgtcg gccggccggc ggcctatgtc gaccagttgc tgatcggcgg ccagggcggc 1440 
gttcaccaca tctggggctg cgaccactac aaaacgacgc tcaatgcgca tgagtttaag 1500 
cgcgtctaca agaagcgcac cgacatggtg aaggacgcca tgagcgctgc cccctacggc 1560 
gaccgcgagg ccatggtcaa tgccattgtc gacgcaatca atcagggcgg attgtttgcc 1620 
gtcaatgtcg acatcatccc gacaaaaatc ggcgaagcct gtcatgttat tctgcctgcg 1680 
gccacgtcag gcgagatgaa cctcacgtca atgaatggcg agcggcgcat gcggctgacc 1740 
gaacgctata tggacccgcc cggtcagtcc atgccggact gcctgattgc cgctcgtctc 1800 
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SEH00122WO - Sequence Listing 30092016 

gccaacacca tggaacgcgt gctgaccgag atgggtgacg tcggctatgc cgctcaattc 1860 
aagggctttg actggcagac agaagaagac gccttcatgg acggctacaa caagaatgca 1920 
catggcggag agttcgtcac ctatgagcgc ctgagtgcga tgggcaccaa cggcttccag 1980 
gagccggcta ccggctttac cgacggcaag atcgagggca cccagcggct ctataccgac 2040 
ggcgtattct cgaccgacga cggcaaggcg cggttcatgg acgcgccatg gcggggactt 2100 
caggcaccgg gcaagcagca gcagaaggac agccacaagt acttgatcaa caacggccgt 2160 
gccaatgtcg tctggcaatc ggcgtatctc gaccaggaaa acgacttcgt catggatcgt 2220 
ttcccctacc cgttcatcga gatgaaccca gaggacatgg cggaagcagg ccttaaggag 2280 
ggcgacctcg tcgagattta caatgatgcc ggagcgacgc aggccatggc ctatccgacg 2340 
ccgacagccc gacgtggaga aaccttcatg ctgttcggtt ttccaaccgg ggttcagggc 2400 
aatgtgacca gtgccgggac gaacgagttg ataatcccga actacaagca gacctggggc 2460 
aatatccgca agatttcgga tgcgcccagg aacgtggctc acctttcctt caagtcgaaa 2520 
gaataccagt cggcttga 2538 

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