                         SEQUENCE LISTING

<110>  Zymeworks Inc.
 
<120>  ANTIGEN-BINDING POLYPEPTIDE CONSTRUCTS COMPRISING KAPPA AND 
       LAMBDA LIGHT CHAINS AND USES THEREOF

<130>  V810793WO

<140>  NOT YET ASSIGNED
<141>  2016-10-07

<150>  US 62/239,206
<151>  2015-10-08

<150>  US 62/261,769
<151>  2015-12-01

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Pertuzumab heavy chain Fab (Domain boundaries: VH; E1 -
       S119, CH1; A120 - V217, Hinge (partial); E218 - T227)

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
    50                  55                  60                  


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys 
    210                 215                 220                 


Thr His Thr 
225         


<210>  2
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Pertuzumab light chain (kappa) (Domain boundaries: VL; 
       D1 - K107, CL; R108 - C214)

<400>  2

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Gly 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  3
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CAT-2200 heavy chain Fab (Domain boundaries: VH; E1 - 
       S118, CH1; A119 - V216, Hinge(partial); E217 - T226)

<400>  3

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Leu Ile His Gly Val Thr Arg Asn Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Gln Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
    210                 215                 220                 


His Thr 
225     


<210>  4
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CAT-2200 light chain (lambda) (Domain boundaries: VL; 
       N1 - L110, CL; G111 - S216)

<400>  4

Asn Phe Met Leu Thr Gln Pro His Ser Val Ser Glu Ser Pro Gly Lys 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Thr Arg Ser Ser Gly Ser Leu Ala Asn Tyr 
            20                  25                  30          


Tyr Val Gln Trp Tyr Gln Gln Arg Pro Gly Ser Ser Pro Thr Ile Val 
        35                  40                  45              


Ile Phe Ala Asn Asn Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Ile Asp Ser Ser Ser Asn Ser Ala Ser Leu Thr Ile Ser Gly 
65                  70                  75                  80  


Leu Lys Thr Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Thr Tyr Asp Pro 
                85                  90                  95      


Tyr Ser Val Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Gln 
            100                 105                 110         


Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu 
        115                 120                 125             


Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr 
    130                 135                 140                 


Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys 
145                 150                 155                 160 


Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr 
                165                 170                 175     


Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His 
            180                 185                 190         


Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys 
        195                 200                 205             


Thr Val Ala Pro Thr Glu Cys Ser 
    210                 215     


<210>  5
<211>  222
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic IgG1 Fc (Domain boundaries: Hinge (partial); C1 - P5, 
       CH2; A6 - K115, CH3; G116 - K222)

<400>  5

Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
1               5                   10                  15      


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
            20                  25                  30          


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
        35                  40                  45              


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
    50                  55                  60                  


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
65                  70                  75                  80  


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
                85                  90                  95      


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
            100                 105                 110         


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
        115                 120                 125             


Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
    130                 135                 140                 


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
145                 150                 155                 160 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
                165                 170                 175     


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
            180                 185                 190         


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
        195                 200                 205             


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220         


<210>  6
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic IgG1 Fc

<400>  6
tgccctccct gtccagctcc agaactgctg ggaggaccta gcgtgttcct gtttccccct       60

aagccaaaag acactctgat gatttccagg actcccgagg tgacctgcgt ggtggtggac      120

gtgtctcacg aggaccccga agtgaagttc aactggtacg tggatggcgt ggaagtgcat      180

aatgctaaga caaaaccaag agaggaacag tacaactcca cttatcgcgt cgtgagcgtg      240

ctgaccgtgc tgcaccagga ctggctgaac gggaaggagt ataagtgcaa agtcagtaat      300

aaggccctgc ctgctccaat cgaaaaaacc atctctaagg ccaaaggcca gccaagggag      360

ccccaggtgt acacactgcc acccagcaga gacgaactga ccaagaacca ggtgtccctg      420

acatgtctgg tgaaaggctt ctatcctagt gatattgctg tggagtggga atcaaatgga      480

cagccagaga acaattacaa gaccacacct ccagtgctgg acagcgatgg cagcttcttc      540

ctgtattcca agctgacagt ggataaatct cgatggcagc aggggaacgt gtttagttgt      600

tcagtgatgc atgaagccct gcacaatcat tacactcaga agagcctgtc cctgtctccc      660

ggcaaa                                                                 666


<210>  7
<211>  681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Pertuzumab heavy chain Fab

<400>  7
gaagtgcagc tggtcgaatc tggaggagga ctggtgcagc caggagggtc cctgcgcctg       60

tcttgcgccg ctagtggctt cacttttacc gactacacca tggattgggt gcgacaggca      120

cctggaaagg gcctggagtg ggtcgccgat gtgaacccaa atagcggagg ctccatctac      180

aaccagcggt tcaagggccg gttcaccctg tcagtggacc ggagcaaaaa caccctgtat      240

ctgcagatga atagcctgcg agccgaagat actgctgtgt actattgcgc ccggaatctg      300

gggccctcct tctactttga ctattggggg cagggaactc tggtcaccgt gagctccgcc      360

tccaccaagg gaccttctgt gttcccactg gctccctcta gtaaatccac atctggggga      420

actgcagccc tgggctgtct ggtgaaggac tacttcccag agcccgtcac agtgtcttgg      480

aacagtggcg ctctgacttc tggggtccac acctttcctg cagtgctgca gtcaagcggg      540

ctgtacagcc tgtcctctgt ggtcaccgtg ccaagttcaa gcctgggaac acagacttat      600

atctgcaacg tgaatcacaa gccatccaat acaaaagtcg acaagaaagt ggaacccaag      660

tcttgtgata aaacccatac a                                                681


<210>  8
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic Pertuzumab light chain (kappa)

<400>  8
gatattcaga tgacccagtc cccaagctcc ctgagtgcct cagtgggcga ccgagtcacc       60

atcacatgca aggcttccca ggatgtgtct attggagtcg catggtacca gcagaagcca      120

ggcaaagcac ccaagctgct gatctatagc gcctcctacc ggtataccgg cgtgccctct      180

agattctctg gcagtgggtc aggaacagac tttactctga ccatctctag tctgcagcct      240

gaggatttcg ctacctacta ttgccagcag tactatatct acccatatac ctttggccag      300

gggacaaaag tggagatcaa gaggactgtg gccgctccct ccgtcttcat ttttccccct      360

tctgacgaac agctgaaaag tggcacagcc agcgtggtct gtctgctgaa caatttctac      420

cctcgcgaag ccaaagtgca gtggaaggtc gataacgctc tgcagagcgg caacagccag      480

gagtctgtga ctgaacagga cagtaaagat tcaacctata gcctgtcaag cacactgact      540

ctgagcaagg cagactacga gaagcacaaa gtgtatgcct gcgaagtcac acatcagggg      600

ctgtcctctc ctgtgactaa gagctttaac agaggagagt gt                         642


<210>  9
<211>  678
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CAT-2200 heavy chain Fab

<400>  9
gaggtgcagc tgctggaatc tggggggggc ctggtgcagc ctggggggtc cctgagactg       60

tcatgtgctg ccagcgggtt tactttcagc tcctacgcta tgtcctgggt gcgacaggca      120

cccgggaagg gactggagtg ggtctctgca atcagtgggt caggcgggag tacttactat      180

gccgacagcg tgaagggacg gttcactatc tcaagagata acagcaagaa caccctgtat      240

ctgcagatga acagcctgag agcagaagac acagccgtgt actattgcgc cagggatctg      300

atccacggag tcactcgcaa ttggggccag gggactctgg tgaccgtctc tagtgctagc      360

acaaaggggc cctctgtgtt tccactggcc ccctcaagca aaagcacatc cggaggaact      420

gcagctctgg gatgtctggt gaaggactac ttcccccagc ctgtgaccgt ctcttggaac      480

agtggagccc tgaccagcgg cgtgcacaca tttcctgctg tcctgcagtc ctctggcctg      540

tactccctga gttcagtggt cacagtgcct agctcctctc tggggaccca gacatatatt      600

tgcaacgtga atcataaacc aagcaacact aaggtcgaca agaaagtgga gcccaagagc      660

tgtgataaaa ctcatacc                                                    678


<210>  10
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CAT-2200 light chain (lambda)

<400>  10
aactttatgc tgactcagcc ccactccgtg tccgagagcc ctggcaaaac tgtgactatt       60

tcatgtaccc gatcatctgg aagcctggcc aactactatg tgcagtggta ccagcagagg      120

ccaggcagct cccccactat cgtgattttc gctaacaatc agcggccttc cggcgtccca      180

gacagatttt ccgggtctat cgattctagt tcaaatagtg catcactgac tatttccggg      240

ctgaagaccg aggacgaagc cgattactat tgccagacct acgaccccta ttctgtggtc      300

ttcggcgggg gaaccaagct gacagtgctg ggacagccaa aagcggcgcc cagtgtcaca      360

ctgtttcccc ctagctccga ggaactgcag gctaacaaag caacactggt gtgtctgatc      420

agcgacttct accctggagc tgtgactgtc gcctggaagg ctgattctag tccagtgaaa      480

gcaggcgtcg agaccacaac tccctctaag cagagtaaca acaagtacgc agcctcaagc      540

tatctgtcac tgaccccaga acagtggaag agccaccgga gctattcctg ccaggtcact      600

cacgaaggct ccactgtcga gaaaaccgtc gctcccaccg aatgttca                   648


<210>  11
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic Human IgG1 Fc sequence 231-447 (EU-numbering), without 
       hinge

<400>  11

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
145                 150                 155                 160 


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                165                 170                 175     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215         


<210>  12
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic SGN-CD19a heavy chain Fab (Domain boundaries: VH; 
       Q1-S120, CH1; A121-V218, Hinge (partial); E219-T228)

<400>  12

Gln Val Thr Leu Arg Glu Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 
1               5                   10                  15      


Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ser 
            20                  25                  30          


Gly Met Gly Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 
        35                  40                  45              


Trp Leu Ala His Ile Trp Trp Asp Asp Asp Lys Arg Tyr Asn Pro Ala 
    50                  55                  60                  


Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 
65                  70                  75                  80  


Val Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala Ala Tyr Tyr 
                85                  90                  95      


Cys Ala Arg Met Glu Leu Trp Ser Tyr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr 
225             


<210>  13
<211>  213
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic SGN-CD19a light chain (kappa) (Domain boundaries: VL; 
       E1-K106, CL; R107-C213)

<400>  13

Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu 
65                  70                  75                  80  


Asp Phe Ala Val Tyr Tyr Cys Phe Gln Gly Ser Val Tyr Pro Phe Thr 
                85                  90                  95      


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         


Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             


Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 


Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 


Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     


Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         


Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             


Asn Arg Gly Glu Cys 
    210             


<210>  14
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CR8071 heavy chain Fab (Domain boundaries: VH; Q1-S125,
       CH1; A126-V223, Hinge (partial); E224-T233)

<400>  14

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Arg Val Ser Cys Arg Ala Ser Gly Tyr Ile Phe Thr Glu Ser 
            20                  25                  30          


Gly Ile Thr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Trp Ile Ser Gly Tyr Ser Gly Asp Thr Lys Tyr Ala Gln Lys Leu 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Lys Asp Thr Ser Thr Thr Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Arg Tyr Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Val Gln Tyr Ser Gly Ser Tyr Leu Gly Ala Tyr Tyr Phe 
            100                 105                 110         


Asp Tyr Trp Ser Pro Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr 
        115                 120                 125             


Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser 
    130                 135                 140                 


Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu 
145                 150                 155                 160 


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170                 175     


Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser 
            180                 185                 190         


Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys 
        195                 200                 205             


Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu 
    210                 215                 220                 


Pro Lys Ser Cys Asp Lys Thr His Thr 
225                 230             


<210>  15
<211>  216
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CR8071 light chain (lambda) (Domain boundaries: VL; 
       Q1-L110, CL; R111-S216)

<400>  15

Gln Ser Val Leu Thr Gln Pro Pro Ser Ala Ser Gly Thr Pro Gly Gln 
1               5                   10                  15      


Arg Val Thr Ile Ser Cys Ser Gly Ser Ser Ser Asn Ile Gly Thr Asn 
            20                  25                  30          


Tyr Val Tyr Trp Tyr Gln Gln Phe Pro Gly Thr Ala Pro Lys Leu Leu 
        35                  40                  45              


Ile Tyr Arg Ser Tyr Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Lys Ser Gly Ser Ser Ala Ser Leu Ala Ile Ser Gly Leu Gln 
65                  70                  75                  80  


Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Thr Trp Asp Asp Ser Leu 
                85                  90                  95      


Asp Gly Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Arg Gln 
            100                 105                 110         


Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu 
        115                 120                 125             


Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr 
    130                 135                 140                 


Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys 
145                 150                 155                 160 


Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr 
                165                 170                 175     


Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His 
            180                 185                 190         


Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys 
        195                 200                 205             


Thr Val Ala Pro Thr Glu Cys Ser 
    210                 215     


<210>  16
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic SGN-CD19a heavy chain Fab

<400>  16
caggtgacac tgagagaatc cggcccagcc ctggtgaagc ccactcagac cctgacactg       60

acttgcacct tctctgggtt ttccctgtct acaagtggga tgggagtggg atggatcagg      120

cagccacctg gaaaagccct ggagtggctg gctcacattt ggtgggacga tgacaagcgg      180

tacaacccag cactgaaaag cagactgaca atcagcaagg atacttccaa aaaccaggtg      240

gtcctgacaa tgactaatat ggaccccgtg gacacagccg cttactattg cgcccgcatg      300

gaactgtgga gctactattt cgactactgg gggcagggaa cactggtcac tgtgagctcc      360

gctagcacta aggggccttc cgtgtttcca ctggctccct ctagtaaatc cacctctgga      420

ggcacagctg cactgggatg tctggtgaag gattacttcc ctgaaccagt cacagtgagt      480

tggaactcag gggctctgac aagtggagtc catacttttc ccgcagtgct gcagtcaagc      540

ggactgtact ccctgtcctc tgtggtcacc gtgcctagtt caagcctggg cacccagaca      600

tatatctgca acgtgaatca caagccatca aatacaaaag tcgacaagaa agtggagccc      660

aagagctgtg ataaaactca tacc                                             684


<210>  17
<211>  639
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic SGN-CD19a light chain (kappa)

<400>  17
gagatcgtgc tgacccagtc tccagccaca ctgtctctga gcccaggaga gagggccacc       60

ctgtcctgct ctgccagctc cagcgtgagc tacatgcact ggtatcagca gaagccagga      120

caggccccta ggctgctgat ctacgacacc agcaagctgg cctccggcat ccccgcaaga      180

ttcagcggct ccggctctgg cacagacttt accctgacaa tcagctccct ggagcctgag      240

gatttcgccg tgtactattg ttttcagggc agcgtgtatc cattcacctt tggccagggc      300

acaaagctgg agatcaagcg gacagtggcg gcgcccagtg tcttcatttt tccccctagc      360

gacgaacagc tgaagtctgg gacagccagt gtggtctgtc tgctgaacaa cttctaccct      420

agagaggcta aagtgcagtg gaaggtcgat aacgcactgc agtccggaaa ttctcaggag      480

agtgtgactg aacaggactc aaaagatagc acctattccc tgtcaagcac actgactctg      540

agcaaggccg actacgagaa gcataaagtg tatgcttgtg aagtcaccca ccaggggctg      600

agttcaccag tcacaaaatc attcaacaga ggggagtgc                             639


<210>  18
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CR8071 heavy chain Fab

<400>  18
caggtgcagc tggtccagtc cggggctgaa gtgaaaaaac ctggggcatc cgtgcgggtg       60

tcatgtcggg caagcgggta tatctttact gagtctggaa tcacctgggt gaggcaggct      120

cccggacagg gactggaatg gatgggatgg atttctggat acagtggcga cacaaagtat      180

gcacagaaac tgcagggccg cgtcaccatg acaaaggata cttcaaccac aactgcctac      240

atggagctgc ggagcctgag atatgacgat acagccgtgt actattgcgc ccgggacgtg      300

cagtacagcg ggtcctacct gggggcatac tacttcgatt actggtcacc tggaactctg      360

gtcaccgtct cttcagctag caccaagggc ccttctgtgt ttccactggc accctcaagc      420

aaaagcacct ccggaggaac agcagcactg ggatgtctgg tcaaggacta tttccccgag      480

cctgtgaccg tctcatggaa tagcggcgca ctgactagtg gggtgcacac ctttcccgcc      540

gtcctgcagt cctctgggct gtacagcctg agttcagtgg tcacagtgcc aagctcctct      600

ctgggaactc agacctatat ctgcaacgtc aatcataaac ccagcaacac aaaggtcgac      660

aagaaagtgg agcccaagag ctgtgataaa actcatacc                             699


<210>  19
<211>  648
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic CR8071 light chain (lambda)

<400>  19
cagagcgtcc tgactcagcc tccctccgcc tccggaacac ctgggcagag agtgactatc       60

tcctgtagcg gatcaagctc aaacattgga accaactacg tgtattggta ccagcagttc      120

cccggcacag ctcctaagct gctgatctat cggagctacc agagaccaag cggggtcccc      180

gacaggtttt ctggcagtaa atcagggagc tccgccagcc tggctatttc cggcctgcag      240

tctgaggacg aagcagatta ctattgcgcc acctgggacg attccctgga tggatgggtc      300

ttcggcggcg gcacaaaact gaccgtcctg aggcagccaa aggcggcgcc cagtgtcaca      360

ctgtttcccc ctagctccga ggaactgcag gctaacaaag caacactggt gtgtctgatc      420

agcgacttct accctggagc tgtgactgtc gcctggaagg ctgattctag tccagtgaaa      480

gcaggcgtcg agaccacaac tccctctaag cagagtaaca acaagtacgc agcctcaagc      540

tatctgtcac tgaccccaga acagtggaag agccaccgga gctattcctg ccaggtcact      600

cacgaaggct ccactgtcga gaaaaccgtc gctcccaccg aatgttca                   648


<210>  20
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic variant Human IgG1 Fc sequence 231-447 (EU-numbering), 
       without hinge


<220>
<221>  MISC_FEATURE
<222>  (120)..(120)
<223>  Xaa is Thr or Val

<220>
<221>  MISC_FEATURE
<222>  (121)..(121)
<223>  Xaa is Leu or Tyr

<220>
<221>  MISC_FEATURE
<222>  (136)..(136)
<223>  Xaa is Thr or Leu

<220>
<221>  MISC_FEATURE
<222>  (160)..(160)
<223>  Xaa is Asn or Arg

<220>
<221>  MISC_FEATURE
<222>  (162)..(162)
<223>  Xaa is Lys, Met or Leu

<220>
<221>  MISC_FEATURE
<222>  (164)..(164)
<223>  Xaa is Thr or Trp

<220>
<221>  MISC_FEATURE
<222>  (170)..(170)
<223>  Xaa is Ser or Glu

<220>
<221>  MISC_FEATURE
<222>  (175)..(175)
<223>  Xaa is Phe or Ala

<220>
<221>  MISC_FEATURE
<222>  (177)..(177)
<223>  Xaa is Tyr or Val

<400>  20

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
            100                 105                 110         


Pro Arg Glu Pro Gln Val Tyr Xaa Xaa Pro Pro Ser Arg Asp Glu Leu 
        115                 120                 125             


Thr Lys Asn Gln Val Ser Leu Xaa Cys Leu Val Lys Gly Phe Tyr Pro 
    130                 135                 140                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Xaa 
145                 150                 155                 160 


Tyr Xaa Thr Xaa Pro Pro Val Leu Asp Xaa Asp Gly Ser Phe Xaa Leu 
                165                 170                 175     


Xaa Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
            180                 185                 190         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        195                 200                 205             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215         


