                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING PROTEASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  12773-WO-PCT

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1257
<212>  DNA
<213>  Bacillus subtilis


<220>
<221>  sig_peptide
<222>  (1)..(81)

<220>
<221>  CDS
<222>  (1)..(1254)

<220>
<221>  mat_peptide
<222>  (319)..(1254)

<400>  1
atg aag  aaa aaa aga ata att  gga tca gcg gta tta agt gtc gca atg       48
Met Lys  Lys Lys Arg Ile Ile  Gly Ser Ala Val Leu Ser Val Ala Met         
    -105                 -100                 -95                         

ggg ctt tct gta ttt gca tca ggg gca ttt ggc cag cag gtt gag agc         96
Gly Leu Ser Val Phe Ala Ser Gly Ala Phe Gly Gln Gln Val Glu Ser           
-90                 -85                 -80                 -75           

aat gaa acc tac cgg gtc gtt att cag gga cca agt gcg gaa aag gca        144
Asn Glu Thr Tyr Arg Val Val Ile Gln Gly Pro Ser Ala Glu Lys Ala           
                -70                 -65                 -60               

aag gca aaa tcg aac tac gga gta cgg tgg gat ttt gga cag aaa ggt        192
Lys Ala Lys Ser Asn Tyr Gly Val Arg Trp Asp Phe Gly Gln Lys Gly           
            -55                 -50                 -45                   

ttt act aca aca gtc aat gct aaa cag tat cag gca ctt tta aaa aat        240
Phe Thr Thr Thr Val Asn Ala Lys Gln Tyr Gln Ala Leu Leu Lys Asn           
        -40                 -35                 -30                       

aaa aac tta aag att gat aga gta gat gaa gtt aag aat gca cct gtg        288
Lys Asn Leu Lys Ile Asp Arg Val Asp Glu Val Lys Asn Ala Pro Val           
    -25                 -20                 -15                           

aca gct gca aaa cct gga tcg ggg gct gca tca gct ccg gca gac ggt        336
Thr Ala Ala Lys Pro Gly Ser Gly Ala Ala Ser Ala Pro Ala Asp Gly           
-10                 -5              -1  1               5                 

aca cct tgg gga att gag gcc att tat aat gac agc tcc atc caa agc        384
Thr Pro Trp Gly Ile Glu Ala Ile Tyr Asn Asp Ser Ser Ile Gln Ser           
            10                  15                  20                    

act tca ggc gga aat ggg gta aaa gtt gcc gtg ctg gac aca ggg gta        432
Thr Ser Gly Gly Asn Gly Val Lys Val Ala Val Leu Asp Thr Gly Val           
        25                  30                  35                        

aat aca gcg cat gct gac ctt gct gga caa gct gaa cag tgt aaa gac        480
Asn Thr Ala His Ala Asp Leu Ala Gly Gln Ala Glu Gln Cys Lys Asp           
    40                  45                  50                            

ttt aca caa aga aag aca cct tta att gac gga agc tgc gga gac aag        528
Phe Thr Gln Arg Lys Thr Pro Leu Ile Asp Gly Ser Cys Gly Asp Lys           
55                  60                  65                  70            

aac gga cac ggc aca cat gtt gcg ggt act gta ctg gct cat ggc ggc        576
Asn Gly His Gly Thr His Val Ala Gly Thr Val Leu Ala His Gly Gly           
                75                  80                  85                

gct aac gga caa ggc gtt tat ggg gtc gct cca gac gca gat ctc tgg        624
Ala Asn Gly Gln Gly Val Tyr Gly Val Ala Pro Asp Ala Asp Leu Trp           
            90                  95                  100                   

gca tat aag gtg tta aat gac aga gga tca ggg tat tct gat gac atc        672
Ala Tyr Lys Val Leu Asn Asp Arg Gly Ser Gly Tyr Ser Asp Asp Ile           
        105                 110                 115                       

gcc gga gct att aag cat gcg gcc gat gaa gcg gtc cgc aca gga tct        720
Ala Gly Ala Ile Lys His Ala Ala Asp Glu Ala Val Arg Thr Gly Ser           
    120                 125                 130                           

aaa gtg gtc att tcc atg tca tta ggt tca agc tct aaa agc aca ttg        768
Lys Val Val Ile Ser Met Ser Leu Gly Ser Ser Ser Lys Ser Thr Leu           
135                 140                 145                 150           

att gca gat gca gtt gat tac gca tac agc aaa ggt gtc ctg gta gtg        816
Ile Ala Asp Ala Val Asp Tyr Ala Tyr Ser Lys Gly Val Leu Val Val           
                155                 160                 165               

gct gct gcc gga aat gat ggt cct gcc gat aac acg att ggc tat cct        864
Ala Ala Ala Gly Asn Asp Gly Pro Ala Asp Asn Thr Ile Gly Tyr Pro           
            170                 175                 180                   

ggc gca ttg gtg aat gct gtt gca gtg gcg gct ctt gaa aat gtt cag        912
Gly Ala Leu Val Asn Ala Val Ala Val Ala Ala Leu Glu Asn Val Gln           
        185                 190                 195                       

caa aat gga tcc tac cgt gtt gca gat ttt tca tca cgg ggc aat cct        960
Gln Asn Gly Ser Tyr Arg Val Ala Asp Phe Ser Ser Arg Gly Asn Pro           
    200                 205                 210                           

gca act gat ggc gac ttc gtg att cag gag cgt gat gtt gag gtg tct       1008
Ala Thr Asp Gly Asp Phe Val Ile Gln Glu Arg Asp Val Glu Val Ser           
215                 220                 225                 230           

gca cca ggc aga gcg att gaa tcc aca tgg tat gat ggc agc tac agc       1056
Ala Pro Gly Arg Ala Ile Glu Ser Thr Trp Tyr Asp Gly Ser Tyr Ser           
                235                 240                 245               

aca atc agc ggc aca tcc atg gct act ccc cat gta tcc gga ttg gct       1104
Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val Ser Gly Leu Ala           
            250                 255                 260                   

gct aag atc tgg gcc caa aat cca tcc atg tcc cat aca cag ctt cgc       1152
Ala Lys Ile Trp Ala Gln Asn Pro Ser Met Ser His Thr Gln Leu Arg           
        265                 270                 275                       

gcc gag ctt cag agc cgg gcc aag caa aat gat att ctt ggc gga aca       1200
Ala Glu Leu Gln Ser Arg Ala Lys Gln Asn Asp Ile Leu Gly Gly Thr           
    280                 285                 290                           

gga gct gcc gct gga gat gat tat gca tca ggc ttt ggc ttc ccg cgt       1248
Gly Ala Ala Ala Gly Asp Asp Tyr Ala Ser Gly Phe Gly Phe Pro Arg           
295                 300                 305                 310           

gta aag taa                                                           1257
Val Lys                                                                   
                                                                          


<210>  2
<211>  418
<212>  PRT
<213>  Bacillus subtilis

<400>  2

Met Lys  Lys Lys Arg Ile Ile  Gly Ser Ala Val Leu Ser Val Ala Met 
    -105                 -100                 -95                 


Gly Leu Ser Val Phe Ala Ser Gly Ala Phe Gly Gln Gln Val Glu Ser 
-90                 -85                 -80                 -75 


Asn Glu Thr Tyr Arg Val Val Ile Gln Gly Pro Ser Ala Glu Lys Ala 
                -70                 -65                 -60     


Lys Ala Lys Ser Asn Tyr Gly Val Arg Trp Asp Phe Gly Gln Lys Gly 
            -55                 -50                 -45         


Phe Thr Thr Thr Val Asn Ala Lys Gln Tyr Gln Ala Leu Leu Lys Asn 
        -40                 -35                 -30             


Lys Asn Leu Lys Ile Asp Arg Val Asp Glu Val Lys Asn Ala Pro Val 
    -25                 -20                 -15                 


Thr Ala Ala Lys Pro Gly Ser Gly Ala Ala Ser Ala Pro Ala Asp Gly 
-10                 -5              -1  1               5       


Thr Pro Trp Gly Ile Glu Ala Ile Tyr Asn Asp Ser Ser Ile Gln Ser 
            10                  15                  20          


Thr Ser Gly Gly Asn Gly Val Lys Val Ala Val Leu Asp Thr Gly Val 
        25                  30                  35              


Asn Thr Ala His Ala Asp Leu Ala Gly Gln Ala Glu Gln Cys Lys Asp 
    40                  45                  50                  


Phe Thr Gln Arg Lys Thr Pro Leu Ile Asp Gly Ser Cys Gly Asp Lys 
55                  60                  65                  70  


Asn Gly His Gly Thr His Val Ala Gly Thr Val Leu Ala His Gly Gly 
                75                  80                  85      


Ala Asn Gly Gln Gly Val Tyr Gly Val Ala Pro Asp Ala Asp Leu Trp 
            90                  95                  100         


Ala Tyr Lys Val Leu Asn Asp Arg Gly Ser Gly Tyr Ser Asp Asp Ile 
        105                 110                 115             


Ala Gly Ala Ile Lys His Ala Ala Asp Glu Ala Val Arg Thr Gly Ser 
    120                 125                 130                 


Lys Val Val Ile Ser Met Ser Leu Gly Ser Ser Ser Lys Ser Thr Leu 
135                 140                 145                 150 


Ile Ala Asp Ala Val Asp Tyr Ala Tyr Ser Lys Gly Val Leu Val Val 
                155                 160                 165     


Ala Ala Ala Gly Asn Asp Gly Pro Ala Asp Asn Thr Ile Gly Tyr Pro 
            170                 175                 180         


Gly Ala Leu Val Asn Ala Val Ala Val Ala Ala Leu Glu Asn Val Gln 
        185                 190                 195             


Gln Asn Gly Ser Tyr Arg Val Ala Asp Phe Ser Ser Arg Gly Asn Pro 
    200                 205                 210                 


Ala Thr Asp Gly Asp Phe Val Ile Gln Glu Arg Asp Val Glu Val Ser 
215                 220                 225                 230 


Ala Pro Gly Arg Ala Ile Glu Ser Thr Trp Tyr Asp Gly Ser Tyr Ser 
                235                 240                 245     


Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val Ser Gly Leu Ala 
            250                 255                 260         


Ala Lys Ile Trp Ala Gln Asn Pro Ser Met Ser His Thr Gln Leu Arg 
        265                 270                 275             


Ala Glu Leu Gln Ser Arg Ala Lys Gln Asn Asp Ile Leu Gly Gly Thr 
    280                 285                 290                 


Gly Ala Ala Ala Gly Asp Asp Tyr Ala Ser Gly Phe Gly Phe Pro Arg 
295                 300                 305                 310 


Val Lys 
        


<210>  3
<211>  312
<212>  PRT
<213>  bacillus subtilis

<400>  3

Ser Ala Pro Ala Asp Gly Thr Pro Trp Gly Ile Glu Ala Ile Tyr Asn 
1               5                   10                  15      


Asp Ser Ser Ile Gln Ser Thr Ser Gly Gly Asn Gly Val Lys Val Ala 
            20                  25                  30          


Val Leu Asp Thr Gly Val Asn Thr Ala His Ala Asp Leu Ala Gly Gln 
        35                  40                  45              


Ala Glu Gln Cys Lys Asp Phe Thr Gln Arg Lys Thr Pro Leu Ile Asp 
    50                  55                  60                  


Gly Ser Cys Gly Asp Lys Asn Gly His Gly Thr His Val Ala Gly Thr 
65                  70                  75                  80  


Val Leu Ala His Gly Gly Ala Asn Gly Gln Gly Val Tyr Gly Val Ala 
                85                  90                  95      


Pro Asp Ala Asp Leu Trp Ala Tyr Lys Val Leu Asn Asp Arg Gly Ser 
            100                 105                 110         


Gly Tyr Ser Asp Asp Ile Ala Gly Ala Ile Lys His Ala Ala Asp Glu 
        115                 120                 125             


Ala Val Arg Thr Gly Ser Lys Val Val Ile Ser Met Ser Leu Gly Ser 
    130                 135                 140                 


Ser Ser Lys Ser Thr Leu Ile Ala Asp Ala Val Asp Tyr Ala Tyr Ser 
145                 150                 155                 160 


Lys Gly Val Leu Val Val Ala Ala Ala Gly Asn Asp Gly Pro Ala Asp 
                165                 170                 175     


Asn Thr Ile Gly Tyr Pro Gly Ala Leu Val Asn Ala Val Ala Val Ala 
            180                 185                 190         


Ala Leu Glu Asn Val Gln Gln Asn Gly Ser Tyr Arg Val Ala Asp Phe 
        195                 200                 205             


Ser Ser Arg Gly Asn Pro Ala Thr Asp Gly Asp Phe Val Ile Gln Glu 
    210                 215                 220                 


Arg Asp Val Glu Val Ser Ala Pro Gly Arg Ala Ile Glu Ser Thr Trp 
225                 230                 235                 240 


Tyr Asp Gly Ser Tyr Ser Thr Ile Ser Gly Thr Ser Met Ala Thr Pro 
                245                 250                 255     


His Val Ser Gly Leu Ala Ala Lys Ile Trp Ala Gln Asn Pro Ser Met 
            260                 265                 270         


Ser His Thr Gln Leu Arg Ala Glu Leu Gln Ser Arg Ala Lys Gln Asn 
        275                 280                 285             


Asp Ile Leu Gly Gly Thr Gly Ala Ala Ala Gly Asp Asp Tyr Ala Ser 
    290                 295                 300                 


Gly Phe Gly Phe Pro Arg Val Lys 
305                 310         


<210>  4
<211>  311
<212>  PRT
<213>  bacillus subtilus

<400>  4

Ala Val Pro Ser Thr Gln Thr Pro Trp Gly Ile Lys Ser Ile Tyr Asn 
1               5                   10                  15      


Asp Gln Ser Ile Thr Lys Thr Thr Gly Gly Ser Gly Ile Lys Val Ala 
            20                  25                  30          


Val Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser 
        35                  40                  45              


Ala Glu Gln Cys Lys Asp Phe Thr Gln Ser Asn Pro Leu Val Asp Gly 
    50                  55                  60                  


Ser Cys Thr Asp Arg Gln Gly His Gly Thr His Val Ala Gly Thr Val 
65                  70                  75                  80  


Leu Ala His Gly Gly Ser Asn Gly Gln Gly Val Tyr Gly Val Ala Pro 
                85                  90                  95      


Gln Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly 
            100                 105                 110         


Tyr Ser Asp Asp Ile Ala Ala Ala Ile Arg His Val Ala Asp Glu Ala 
        115                 120                 125             


Ser Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser 
    130                 135                 140                 


Ala Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys 
145                 150                 155                 160 


Gly Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn 
                165                 170                 175     


Thr Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala 
            180                 185                 190         


Leu Glu Asn Val Gln Gln Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser 
        195                 200                 205             


Ser Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Ile Gln Glu Arg 
    210                 215                 220                 


Asp Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr 
225                 230                 235                 240 


Thr Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His 
                245                 250                 255     


Val Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser 
            260                 265                 270         


His Ser Gln Leu Arg Thr Glu Leu Gln Asn Arg Ala Lys Val Tyr Asp 
        275                 280                 285             


Ile Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly 
    290                 295                 300                 


Phe Gly Tyr Pro Arg Val Lys 
305                 310     


<210>  5
<211>  269
<212>  PRT
<213>  bacillus lentus

<400>  5

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  6
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  6
gttcatcgat cgcatcggct cagcaggttg agagcaatga                             40


<210>  7
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  7
gcgttttttt attgattaac gcgtttactt tacacgcggg aagc                        44


<210>  8
<211>  27
<212>  PRT
<213>  Artificial

<220>
<223>  Bacillus Clausii secretion signal

<400>  8

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala 
            20                  25          


