                         SEQUENCE LISTING

<110>  E. I. du Pont de Nemours and Company
       Frisch, Ryan L.
 
<120>  PROTECTED DNA TEMPLATES FOR GENE MODIFICATION AND INCREASED 
       HOMOLOGOUS RECOMBINATION IN CELLS AND METHODS OF USE

<130>  CL6441-WO-PCT

<150>  US 62/240,140 
<151>  2015-10-12

<160>  72    

<170>  PatentIn version 3.5

<210>  1
<211>  1372
<212>  PRT
<213>  Streptococcus pyogenes

<400>  1

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             
Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             
Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             
Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             
Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             
Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             
Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             
Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             
Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             
Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             
Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             
Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             
Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             
Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             
Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             
Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             
Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             
Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             
His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             
Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             
Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             
Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             
Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             
Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             
Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             
Ser Arg  Ala Asp 
    1370         

<210>  2
<211>  4140
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Yarrowia codon optimized Cas9

<400>  2
atggacaaga aatactccat cggcctggac attggaacca actctgtcgg ctgggctgtc       60
atcaccgacg agtacaaggt gccctccaag aaattcaagg tcctcggaaa caccgatcga      120
cactccatca agaaaaacct cattggtgcc ctgttgttcg attctggcga gactgccgaa      180
gctaccagac tcaagcgaac tgctcggcga cgttacaccc gacggaagaa ccgaatctgc      240
tacctgcagg agatcttttc caacgagatg gccaaggtgg acgattcgtt ctttcatcga      300
ctggaggaat ccttcctcgt cgaggaagac aagaaacacg agcgtcatcc catctttggc      360
aacattgtgg acgaggttgc ttaccacgag aagtatccta ccatctacca tctccgaaag      420
aaactcgtcg attccaccga caaggcggat ctcagactta tctacctcgc tctggcacac      480
atgatcaagt ttcgaggtca tttcctcatc gagggcgatc tcaatcccga caacagcgat      540
gtggacaagc tgttcattca gctcgttcag acctacaacc agctgttcga ggaaaacccc      600
atcaatgcct ccggagtcga tgcaaaggcc atcttgtctg ctcgactctc gaagagcaga      660
cgactggaga acctcattgc ccaacttcct ggcgagaaaa agaacggact gtttggcaac      720
ctcattgccc tttctcttgg tctcacaccc aacttcaagt ccaacttcga tctggcggag      780
gacgccaagc tccagctgtc caaggacacc tacgacgatg acctcgacaa cctgcttgca      840
cagattggcg atcagtacgc cgacctgttt ctcgctgcca agaacctttc ggatgctatt      900
ctcttgtctg acattctgcg agtcaacacc gagatcacaa aggctcccct ttctgcctcc      960
atgatcaagc gatacgacga gcaccatcag gatctcacac tgctcaaggc tcttgtccga     1020
cagcaactgc ccgagaagta caaggagatc tttttcgatc agtcgaagaa cggctacgct     1080
ggatacatcg acggcggagc ctctcaggaa gagttctaca agttcatcaa gccaattctc     1140
gagaagatgg acggaaccga ggaactgctt gtcaagctca atcgagagga tctgcttcgg     1200
aagcaacgaa ccttcgacaa cggcagcatt cctcatcaga tccacctcgg tgagctgcac     1260
gccattcttc gacgtcagga agacttctac ccctttctca aggacaaccg agagaagatc     1320
gagaagattc ttacctttcg aatcccctac tatgttggtc ctcttgccag aggaaactct     1380
cgatttgctt ggatgactcg aaagtccgag gaaaccatca ctccctggaa cttcgaggaa     1440
gtcgtggaca agggtgcctc tgcacagtcc ttcatcgagc gaatgaccaa cttcgacaag     1500
aatctgccca acgagaaggt tcttcccaag cattcgctgc tctacgagta ctttacagtc     1560
tacaacgaac tcaccaaagt caagtacgtt accgagggaa tgcgaaagcc tgccttcttg     1620
tctggcgaac agaagaaagc cattgtcgat ctcctgttca agaccaaccg aaaggtcact     1680
gttaagcagc tcaaggagga ctacttcaag aaaatcgagt gtttcgacag cgtcgagatt     1740
tccggagttg aggaccgatt caacgcctct ttgggcacct atcacgatct gctcaagatt     1800
atcaaggaca aggattttct cgacaacgag gaaaacgagg acattctgga ggacatcgtg     1860
ctcactctta ccctgttcga agatcgggag atgatcgagg aacgactcaa gacatacgct     1920
cacctgttcg acgacaaggt catgaaacaa ctcaagcgac gtagatacac cggctgggga     1980
agactttcgc gaaagctcat caacggcatc agagacaagc agtccggaaa gaccattctg     2040
gactttctca agtccgatgg ctttgccaac cgaaacttca tgcagctcat tcacgacgat     2100
tctcttacct tcaaggagga catccagaag gcacaagtgt ccggtcaggg cgacagcttg     2160
cacgaacata ttgccaacct ggctggttcg ccagccatca agaaaggcat tctccagact     2220
gtcaaggttg tcgacgagct ggtgaaggtc atgggacgtc acaagcccga gaacattgtg     2280
atcgagatgg ccagagagaa ccagacaact caaaagggtc agaaaaactc gcgagagcgg     2340
atgaagcgaa tcgaggaagg catcaaggag ctgggatccc agattctcaa ggagcatccc     2400
gtcgagaaca ctcaactgca gaacgagaag ctgtatctct actatctgca gaatggtcga     2460
gacatgtacg tggatcagga actggacatc aatcgtctca gcgactacga tgtggaccac     2520
attgtccctc aatcctttct caaggacgat tctatcgaca acaaggtcct tacacgatcc     2580
gacaagaaca gaggcaagtc ggacaacgtt cccagcgaag aggtggtcaa aaagatgaag     2640
aactactggc gacagctgct caacgccaag ctcattaccc agcgaaagtt cgacaatctt     2700
accaaggccg agcgaggcgg tctgtccgag ctcgacaagg ctggcttcat caagcgtcaa     2760
ctcgtcgaga ccagacagat cacaaagcac gtcgcacaga ttctcgattc tcggatgaac     2820
accaagtacg acgagaacga caagctcatc cgagaggtca aggtgattac tctcaagtcc     2880
aaactggtct ccgatttccg aaaggacttt cagttctaca aggtgcgaga gatcaacaat     2940
taccaccatg cccacgatgc ttacctcaac gccgtcgttg gcactgcgct catcaagaaa     3000
taccccaagc tcgaaagcga gttcgtttac ggcgattaca aggtctacga cgttcgaaag     3060
atgattgcca agtccgaaca ggagattggc aaggctactg ccaagtactt cttttactcc     3120
aacatcatga actttttcaa gaccgagatc accttggcca acggagagat tcgaaagaga     3180
ccacttatcg agaccaacgg cgaaactgga gagatcgtgt gggacaaggg tcgagacttt     3240
gcaaccgtgc gaaaggttct gtcgatgcct caggtcaaca tcgtcaagaa aaccgaggtt     3300
cagactggcg gattctccaa ggagtcgatt ctgcccaagc gaaactccga caagctcatc     3360
gctcgaaaga aagactggga tcccaagaaa tacggtggct tcgattctcc taccgtcgcc     3420
tattccgtgc ttgtcgttgc gaaggtcgag aagggcaagt ccaaaaagct caagtccgtc     3480
aaggagctgc tcggaattac catcatggag cgatcgagct tcgagaagaa tcccatcgac     3540
ttcttggaag ccaagggtta caaggaggtc aagaaagacc tcattatcaa gctgcccaag     3600
tactctctgt tcgaactgga gaacggtcga aagcgtatgc tcgcctccgc tggcgagctg     3660
cagaagggaa acgagcttgc cttgccttcg aagtacgtca actttctcta tctggcttct     3720
cactacgaga agctcaaggg ttctcccgag gacaacgaac agaagcaact cttcgttgag     3780
cagcacaaac attacctcga cgagattatc gagcagattt ccgagttttc gaagcgagtc     3840
atcctggctg atgccaactt ggacaaggtg ctctctgcct acaacaagca tcgggacaaa     3900
cccattcgag aacaggcgga gaacatcatt cacctgttta ctcttaccaa cctgggtgct     3960
cctgcagctt tcaagtactt cgataccact atcgaccgaa agcggtacac atccaccaag     4020
gaggttctcg atgccaccct gattcaccag tccatcactg gcctgtacga gacccgaatc     4080
gacctgtctc agcttggtgg cgactccaga gccgatccca agaaaaagcg aaaggtctaa     4140

<210>  3
<211>  7
<212>  PRT
<213>  SV40

<400>  3
Pro Lys Lys Lys Arg Lys Val 
1               5           

<210>  4
<211>  543
<212>  DNA
<213>  YArrowia lipolytica

<400>  4
tcgacgttta aaccatcatc taagggcctc aaaactacct cggaactgct gcgctgatct       60
ggacaccaca gaggttccga gcactttagg ttgcaccaaa tgtcccacca ggtgcaggca      120
gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa agtgagggcg ctgaggtcga      180
gcagggtggt gtgacttgtt atagccttta gagctgcgaa agcgcgtatg gatttggctc      240
atcaggccag attgagggtc tgtggacaca tgtcatgtta gtgtacttca atcgccccct      300
ggatatagcc ccgacaatag gccgtggcct catttttttg ccttccgcac atttccattg      360
ctcggtaccc acaccttgct tctcctgcac ttgccaacct taatactggt ttacattgac      420
caacatctta caagcggggg gcttgtctag ggtatatata aacagtggct ctcccaatcg      480
gttgccagtc tcttttttcc tttctttccc cacagattcg aaatctaaac tacacatcac      540
acc                                                                    543

<210>  5
<211>  4683
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Yarrowia optimized expression cassette

<400>  5
tcgacgttta aaccatcatc taagggcctc aaaactacct cggaactgct gcgctgatct       60
ggacaccaca gaggttccga gcactttagg ttgcaccaaa tgtcccacca ggtgcaggca      120
gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa agtgagggcg ctgaggtcga      180
gcagggtggt gtgacttgtt atagccttta gagctgcgaa agcgcgtatg gatttggctc      240
atcaggccag attgagggtc tgtggacaca tgtcatgtta gtgtacttca atcgccccct      300
ggatatagcc ccgacaatag gccgtggcct catttttttg ccttccgcac atttccattg      360
ctcggtaccc acaccttgct tctcctgcac ttgccaacct taatactggt ttacattgac      420
caacatctta caagcggggg gcttgtctag ggtatatata aacagtggct ctcccaatcg      480
gttgccagtc tcttttttcc tttctttccc cacagattcg aaatctaaac tacacatcac      540
accatggaca agaaatactc catcggcctg gacattggaa ccaactctgt cggctgggct      600
gtcatcaccg acgagtacaa ggtgccctcc aagaaattca aggtcctcgg aaacaccgat      660
cgacactcca tcaagaaaaa cctcattggt gccctgttgt tcgattctgg cgagactgcc      720
gaagctacca gactcaagcg aactgctcgg cgacgttaca cccgacggaa gaaccgaatc      780
tgctacctgc aggagatctt ttccaacgag atggccaagg tggacgattc gttctttcat      840
cgactggagg aatccttcct cgtcgaggaa gacaagaaac acgagcgtca tcccatcttt      900
ggcaacattg tggacgaggt tgcttaccac gagaagtatc ctaccatcta ccacctgcga      960
aagaaactcg tcgattccac cgacaaggcg gatctcagac ttatctacct cgctctggca     1020
cacatgatca agtttcgagg tcatttcctc atcgagggcg atctcaatcc cgacaacagc     1080
gatgtggaca agctgttcat tcagctcgtt cagacctaca accagctgtt cgaggaaaac     1140
cccatcaatg cctccggagt cgatgcaaag gccatcttgt ctgctcgact ctcgaagagc     1200
agacgactgg agaacctcat tgcccaactt cctggcgaga aaaagaacgg actgtttggc     1260
aacctcattg ccctttctct tggtctcaca cccaacttca agtccaactt cgatctggcg     1320
gaggacgcca agctccagct gtccaaggac acctacgacg atgacctcga caacctgctt     1380
gcacagattg gcgatcagta cgccgacctg tttctcgctg ccaagaacct ttcggatgct     1440
attctcttgt ctgacattct gcgagtcaac accgagatca caaaggctcc cctttctgcc     1500
tccatgatca agcgatacga cgagcaccat caggatctca cactgctcaa ggctcttgtc     1560
cgacagcaac tgcccgagaa gtacaaggag atctttttcg atcagtcgaa gaacggctac     1620
gctggataca tcgacggcgg agcctctcag gaagagttct acaagttcat caagccaatt     1680
ctcgagaaga tggacggaac cgaggaactg cttgtcaagc tcaatcgaga ggatctgctt     1740
cggaagcaac gaaccttcga caacggcagc attcctcatc agatccacct cggtgagctg     1800
cacgccattc ttcgacgtca ggaagacttc tacccctttc tcaaggacaa ccgagagaag     1860
atcgagaaga ttcttacctt tcgaatcccc tactatgttg gtcctcttgc cagaggaaac     1920
tctcgatttg cttggatgac tcgaaagtcc gaggaaacca tcactccctg gaacttcgag     1980
gaagtcgtgg acaagggtgc ctctgcacag tccttcatcg agcgaatgac caacttcgac     2040
aagaatctgc ccaacgagaa ggttcttccc aagcattcgc tgctctacga gtactttaca     2100
gtctacaacg aactcaccaa agtcaagtac gttaccgagg gaatgcgaaa gcctgccttc     2160
ttgtctggcg aacagaagaa agccattgtc gatctcctgt tcaagaccaa ccgaaaggtc     2220
actgttaagc agctcaagga ggactacttc aagaaaatcg agtgtttcga cagcgtcgag     2280
atttccggag ttgaggaccg attcaacgcc tctttgggca cctatcacga tctgctcaag     2340
attatcaagg acaaggattt tctcgacaac gaggaaaacg aggacattct ggaggacatc     2400
gtgctcactc ttaccctgtt cgaagatcgg gagatgatcg aggaacgact caagacatac     2460
gctcacctgt tcgacgacaa ggtcatgaaa caactcaagc gacgtagata caccggctgg     2520
ggaagacttt cgcgaaagct catcaacggc atcagagaca agcagtccgg aaagaccatt     2580
ctggactttc tcaagtccga tggctttgcc aaccgaaact tcatgcagct cattcacgac     2640
gattctctta ccttcaagga ggacatccag aaggcacaag tgtccggtca gggcgacagc     2700
ttgcacgaac atattgccaa cctggctggt tcgccagcca tcaagaaagg cattctccag     2760
actgtcaagg ttgtcgacga gctggtgaag gtcatgggac gtcacaagcc cgagaacatt     2820
gtgatcgaga tggccagaga gaaccagaca actcaaaagg gtcagaaaaa ctcgcgagag     2880
cggatgaagc gaatcgagga aggcatcaag gagctgggat cccagattct caaggagcat     2940
cccgtcgaga acactcaact gcagaacgag aagctgtatc tctactatct gcagaatggt     3000
cgagacatgt acgtggatca ggaactggac atcaatcgtc tcagcgacta cgatgtggac     3060
cacattgtcc ctcaatcctt tctcaaggac gattctatcg acaacaaggt ccttacacga     3120
tccgacaaga acagaggcaa gtcggacaac gttcccagcg aagaggtggt caaaaagatg     3180
aagaactact ggcgacagct gctcaacgcc aagctcatta cccagcgaaa gttcgacaat     3240
cttaccaagg ccgagcgagg cggtctgtcc gagctcgaca aggctggctt catcaagcgt     3300
caactcgtcg agaccagaca gatcacaaag cacgtcgcac agattctcga ttctcggatg     3360
aacaccaagt acgacgagaa cgacaagctc atccgagagg tcaaggtgat tactctcaag     3420
tccaaactgg tctccgattt ccgaaaggac tttcagttct acaaggtgcg agagatcaac     3480
aattaccacc atgcccacga tgcttacctc aacgccgtcg ttggcactgc gctcatcaag     3540
aaatacccca agctcgaaag cgagttcgtt tacggcgatt acaaggtcta cgacgttcga     3600
aagatgattg ccaagtccga acaggagatt ggcaaggcta ctgccaagta cttcttttac     3660
tccaacatca tgaacttttt caagaccgag atcaccttgg ccaacggaga gattcgaaag     3720
agaccactta tcgagaccaa cggcgaaact ggagagatcg tgtgggacaa gggtcgagac     3780
tttgcaaccg tgcgaaaggt tctgtcgatg cctcaggtca acatcgtcaa gaaaaccgag     3840
gttcagactg gcggattctc caaggagtcg attctgccca agcgaaactc cgacaagctc     3900
atcgctcgaa agaaagactg ggatcccaag aaatacggtg gcttcgattc tcctaccgtc     3960
gcctattccg tgcttgtcgt tgcgaaggtc gagaagggca agtccaaaaa gctcaagtcc     4020
gtcaaggagc tgctcggaat taccatcatg gagcgatcga gcttcgagaa gaatcccatc     4080
gacttcttgg aagccaaggg ttacaaggag gtcaagaaag acctcattat caagctgccc     4140
aagtactctc tgttcgaact ggagaacggt cgaaagcgta tgctcgcctc cgctggcgag     4200
ctgcagaagg gaaacgagct tgccttgcct tcgaagtacg tcaactttct ctatctggct     4260
tctcactacg agaagctcaa gggttctccc gaggacaacg aacagaagca actcttcgtt     4320
gagcagcaca aacattacct cgacgagatt atcgagcaga tttccgagtt ttcgaagcga     4380
gtcatcctgg ctgatgccaa cttggacaag gtgctctctg cctacaacaa gcatcgggac     4440
aaacccattc gagaacaggc ggagaacatc attcacctgt ttactcttac caacctgggt     4500
gctcctgcag ctttcaagta cttcgatacc actatcgacc gaaagcggta cacatccacc     4560
aaggaggttc tcgatgccac cctgattcac cagtccatca ctggcctgta cgagacccga     4620
atcgacctgt ctcagcttgg tggcgactcc agagccgatc ccaagaaaaa gcgaaaggtc     4680
taa                                                                   4683

<210>  6
<211>  10706
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pZufCas9

<400>  6
catggacaag aaatactcca tcggcctgga cattggaacc aactctgtcg gctgggctgt       60
catcaccgac gagtacaagg tgccctccaa gaaattcaag gtcctcggaa acaccgatcg      120
acactccatc aagaaaaacc tcattggtgc cctgttgttc gattctggcg agactgccga      180
agctaccaga ctcaagcgaa ctgctcggcg acgttacacc cgacggaaga accgaatctg      240
ctacctgcag gagatctttt ccaacgagat ggccaaggtg gacgattcgt tctttcatcg      300
actggaggaa tccttcctcg tcgaggaaga caagaaacac gagcgtcatc ccatctttgg      360
caacattgtg gacgaggttg cttaccacga gaagtatcct accatctacc acctgcgaaa      420
gaaactcgtc gattccaccg acaaggcgga tctcagactt atctacctcg ctctggcaca      480
catgatcaag tttcgaggtc atttcctcat cgagggcgat ctcaatcccg acaacagcga      540
tgtggacaag ctgttcattc agctcgttca gacctacaac cagctgttcg aggaaaaccc      600
catcaatgcc tccggagtcg atgcaaaggc catcttgtct gctcgactct cgaagagcag      660
acgactggag aacctcattg cccaacttcc tggcgagaaa aagaacggac tgtttggcaa      720
cctcattgcc ctttctcttg gtctcacacc caacttcaag tccaacttcg atctggcgga      780
ggacgccaag ctccagctgt ccaaggacac ctacgacgat gacctcgaca acctgcttgc      840
acagattggc gatcagtacg ccgacctgtt tctcgctgcc aagaaccttt cggatgctat      900
tctcttgtct gacattctgc gagtcaacac cgagatcaca aaggctcccc tttctgcctc      960
catgatcaag cgatacgacg agcaccatca ggatctcaca ctgctcaagg ctcttgtccg     1020
acagcaactg cccgagaagt acaaggagat ctttttcgat cagtcgaaga acggctacgc     1080
tggatacatc gacggcggag cctctcagga agagttctac aagttcatca agccaattct     1140
cgagaagatg gacggaaccg aggaactgct tgtcaagctc aatcgagagg atctgcttcg     1200
gaagcaacga accttcgaca acggcagcat tcctcatcag atccacctcg gtgagctgca     1260
cgccattctt cgacgtcagg aagacttcta cccctttctc aaggacaacc gagagaagat     1320
cgagaagatt cttacctttc gaatccccta ctatgttggt cctcttgcca gaggaaactc     1380
tcgatttgct tggatgactc gaaagtccga ggaaaccatc actccctgga acttcgagga     1440
agtcgtggac aagggtgcct ctgcacagtc cttcatcgag cgaatgacca acttcgacaa     1500
gaatctgccc aacgagaagg ttcttcccaa gcattcgctg ctctacgagt actttacagt     1560
ctacaacgaa ctcaccaaag tcaagtacgt taccgaggga atgcgaaagc ctgccttctt     1620
gtctggcgaa cagaagaaag ccattgtcga tctcctgttc aagaccaacc gaaaggtcac     1680
tgttaagcag ctcaaggagg actacttcaa gaaaatcgag tgtttcgaca gcgtcgagat     1740
ttccggagtt gaggaccgat tcaacgcctc tttgggcacc tatcacgatc tgctcaagat     1800
tatcaaggac aaggattttc tcgacaacga ggaaaacgag gacattctgg aggacatcgt     1860
gctcactctt accctgttcg aagatcggga gatgatcgag gaacgactca agacatacgc     1920
tcacctgttc gacgacaagg tcatgaaaca actcaagcga cgtagataca ccggctgggg     1980
aagactttcg cgaaagctca tcaacggcat cagagacaag cagtccggaa agaccattct     2040
ggactttctc aagtccgatg gctttgccaa ccgaaacttc atgcagctca ttcacgacga     2100
ttctcttacc ttcaaggagg acatccagaa ggcacaagtg tccggtcagg gcgacagctt     2160
gcacgaacat attgccaacc tggctggttc gccagccatc aagaaaggca ttctccagac     2220
tgtcaaggtt gtcgacgagc tggtgaaggt catgggacgt cacaagcccg agaacattgt     2280
gatcgagatg gccagagaga accagacaac tcaaaagggt cagaaaaact cgcgagagcg     2340
gatgaagcga atcgaggaag gcatcaagga gctgggatcc cagattctca aggagcatcc     2400
cgtcgagaac actcaactgc agaacgagaa gctgtatctc tactatctgc agaatggtcg     2460
agacatgtac gtggatcagg aactggacat caatcgtctc agcgactacg atgtggacca     2520
cattgtccct caatcctttc tcaaggacga ttctatcgac aacaaggtcc ttacacgatc     2580
cgacaagaac agaggcaagt cggacaacgt tcccagcgaa gaggtggtca aaaagatgaa     2640
gaactactgg cgacagctgc tcaacgccaa gctcattacc cagcgaaagt tcgacaatct     2700
taccaaggcc gagcgaggcg gtctgtccga gctcgacaag gctggcttca tcaagcgtca     2760
actcgtcgag accagacaga tcacaaagca cgtcgcacag attctcgatt ctcggatgaa     2820
caccaagtac gacgagaacg acaagctcat ccgagaggtc aaggtgatta ctctcaagtc     2880
caaactggtc tccgatttcc gaaaggactt tcagttctac aaggtgcgag agatcaacaa     2940
ttaccaccat gcccacgatg cttacctcaa cgccgtcgtt ggcactgcgc tcatcaagaa     3000
ataccccaag ctcgaaagcg agttcgttta cggcgattac aaggtctacg acgttcgaaa     3060
gatgattgcc aagtccgaac aggagattgg caaggctact gccaagtact tcttttactc     3120
caacatcatg aactttttca agaccgagat caccttggcc aacggagaga ttcgaaagag     3180
accacttatc gagaccaacg gcgaaactgg agagatcgtg tgggacaagg gtcgagactt     3240
tgcaaccgtg cgaaaggttc tgtcgatgcc tcaggtcaac atcgtcaaga aaaccgaggt     3300
tcagactggc ggattctcca aggagtcgat tctgcccaag cgaaactccg acaagctcat     3360
cgctcgaaag aaagactggg atcccaagaa atacggtggc ttcgattctc ctaccgtcgc     3420
ctattccgtg cttgtcgttg cgaaggtcga gaagggcaag tccaaaaagc tcaagtccgt     3480
caaggagctg ctcggaatta ccatcatgga gcgatcgagc ttcgagaaga atcccatcga     3540
cttcttggaa gccaagggtt acaaggaggt caagaaagac ctcattatca agctgcccaa     3600
gtactctctg ttcgaactgg agaacggtcg aaagcgtatg ctcgcctccg ctggcgagct     3660
gcagaaggga aacgagcttg ccttgccttc gaagtacgtc aactttctct atctggcttc     3720
tcactacgag aagctcaagg gttctcccga ggacaacgaa cagaagcaac tcttcgttga     3780
gcagcacaaa cattacctcg acgagattat cgagcagatt tccgagtttt cgaagcgagt     3840
catcctggct gatgccaact tggacaaggt gctctctgcc tacaacaagc atcgggacaa     3900
acccattcga gaacaggcgg agaacatcat tcacctgttt actcttacca acctgggtgc     3960
tcctgcagct ttcaagtact tcgataccac tatcgaccga aagcggtaca catccaccaa     4020
ggaggttctc gatgccaccc tgattcacca gtccatcact ggcctgtacg agacccgaat     4080
cgacctgtct cagcttggtg gcgactccag agccgatccc aagaaaaagc gaaaggtcta     4140
agcggccgca agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc     4200
caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc     4260
aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc aagtacaata     4320
ctaaacatac tgtacatact catactcgta cccgggcaac ggtttcactt gagtgcagtg     4380
gctagtgctc ttactcgtac agtgtgcaat actgcgtatc atagtctttg atgtatatcg     4440
tattcattca tgttagttgc gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct     4500
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     4560
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta     4620
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     4680
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     4740
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     4800
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     4860
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     4920
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     4980
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     5040
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     5100
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     5160
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     5220
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     5280
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     5340
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     5400
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     5460
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     5520
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     5580
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     5640
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     5700
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     5760
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     5820
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     5880
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     5940
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     6000
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     6060
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     6120
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     6180
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     6240
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     6300
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     6360
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     6420
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     6480
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     6540
ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     6600
tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     6660
tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     6720
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     6780
gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     6840
agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     6900
cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     6960
agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca     7020
ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     7080
acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     7140
ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg     7200
cgaattgggt accgggcccc ccctcgaggt cgatggtgtc gataagcttg atatcgaatt     7260
catgtcacac aaaccgatct tcgcctcaag gaaacctaat tctacatccg agagactgcc     7320
gagatccagt ctacactgat taattttcgg gccaataatt taaaaaaatc gtgttatata     7380
atattatatg tattatatat atacatcatg atgatactga cagtcatgtc ccattgctaa     7440
atagacagac tccatctgcc gcctccaact gatgttctca atatttaagg ggtcatctcg     7500
cattgtttaa taataaacag actccatcta ccgcctccaa atgatgttct caaaatatat     7560
tgtatgaact tatttttatt acttagtatt attagacaac ttacttgctt tatgaaaaac     7620
acttcctatt taggaaacaa tttataatgg cagttcgttc atttaacaat ttatgtagaa     7680
taaatgttat aaatgcgtat gggaaatctt aaatatggat agcataaatg atatctgcat     7740
tgcctaattc gaaatcaaca gcaacgaaaa aaatcccttg tacaacataa atagtcatcg     7800
agaaatatca actatcaaag aacagctatt cacacgttac tattgagatt attattggac     7860
gagaatcaca cactcaactg tctttctctc ttctagaaat acaggtacaa gtatgtacta     7920
ttctcattgt tcatacttct agtcatttca tcccacatat tccttggatt tctctccaat     7980
gaatgacatt ctatcttgca aattcaacaa ttataataag atataccaaa gtagcggtat     8040
agtggcaatc aaaaagcttc tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc     8100
attaaaggta tatatttatt tcttgttata taatcctttt gtttattaca tgggctggat     8160
acataaaggt attttgattt aattttttgc ttaaattcaa tcccccctcg ttcagtgtca     8220
actgtaatgg taggaaatta ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa     8280
aatcgtattt ccaggttaga cgttccgcag aatctagaat gcggtatgcg gtacattgtt     8340
cttcgaacgt aaaagttgcg ctccctgaga tattgtacat ttttgctttt acaagtacaa     8400
gtacatcgta caactatgta ctactgttga tgcatccaca acagtttgtt ttgttttttt     8460
ttgttttttt tttttctaat gattcattac cgctatgtat acctacttgt acttgtagta     8520
agccgggtta ttggcgttca attaatcata gacttatgaa tctgcacggt gtgcgctgcg     8580
agttactttt agcttatgca tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa     8640
cggatgctca atcgatttcg acagtaatta attaagtcat acacaagtca gctttcttcg     8700
agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     8760
agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     8820
atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     8880
ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     8940
atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     9000
cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     9060
tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     9120
ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     9180
accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     9240
gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     9300
ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     9360
ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     9420
ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     9480
tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     9540
tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     9600
atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     9660
agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     9720
agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     9780
ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     9840
accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     9900
agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     9960
gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg    10020
atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa    10080
gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc    10140
aatgacgagt cagacagata ctcgtcgacg tttaaaccat catctaaggg cctcaaaact    10200
acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10260
caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10320
aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10380
cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10440
gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10500
tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10560
accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10620
tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    10680
ttcgaaatct aaactacaca tcacac                                         10706

<210>  7
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AarI-removal 1

<400>  7
agaagtatcc taccatctac catctccgaa agaaactcgt cgattcc                     47

<210>  8
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AarI-removal 2

<400>  8
ggaatcgacg agtttctttc ggagatggta gatggtagga tacttct                     47

<210>  9
<211>  10706
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF109

<400>  9
catggacaag aaatactcca tcggcctgga cattggaacc aactctgtcg gctgggctgt       60
catcaccgac gagtacaagg tgccctccaa gaaattcaag gtcctcggaa acaccgatcg      120
acactccatc aagaaaaacc tcattggtgc cctgttgttc gattctggcg agactgccga      180
agctaccaga ctcaagcgaa ctgctcggcg acgttacacc cgacggaaga accgaatctg      240
ctacctgcag gagatctttt ccaacgagat ggccaaggtg gacgattcgt tctttcatcg      300
actggaggaa tccttcctcg tcgaggaaga caagaaacac gagcgtcatc ccatctttgg      360
caacattgtg gacgaggttg cttaccacga gaagtatcct accatctacc atctccgaaa      420
gaaactcgtc gattccaccg acaaggcgga tctcagactt atctacctcg ctctggcaca      480
catgatcaag tttcgaggtc atttcctcat cgagggcgat ctcaatcccg acaacagcga      540
tgtggacaag ctgttcattc agctcgttca gacctacaac cagctgttcg aggaaaaccc      600
catcaatgcc tccggagtcg atgcaaaggc catcttgtct gctcgactct cgaagagcag      660
acgactggag aacctcattg cccaacttcc tggcgagaaa aagaacggac tgtttggcaa      720
cctcattgcc ctttctcttg gtctcacacc caacttcaag tccaacttcg atctggcgga      780
ggacgccaag ctccagctgt ccaaggacac ctacgacgat gacctcgaca acctgcttgc      840
acagattggc gatcagtacg ccgacctgtt tctcgctgcc aagaaccttt cggatgctat      900
tctcttgtct gacattctgc gagtcaacac cgagatcaca aaggctcccc tttctgcctc      960
catgatcaag cgatacgacg agcaccatca ggatctcaca ctgctcaagg ctcttgtccg     1020
acagcaactg cccgagaagt acaaggagat ctttttcgat cagtcgaaga acggctacgc     1080
tggatacatc gacggcggag cctctcagga agagttctac aagttcatca agccaattct     1140
cgagaagatg gacggaaccg aggaactgct tgtcaagctc aatcgagagg atctgcttcg     1200
gaagcaacga accttcgaca acggcagcat tcctcatcag atccacctcg gtgagctgca     1260
cgccattctt cgacgtcagg aagacttcta cccctttctc aaggacaacc gagagaagat     1320
cgagaagatt cttacctttc gaatccccta ctatgttggt cctcttgcca gaggaaactc     1380
tcgatttgct tggatgactc gaaagtccga ggaaaccatc actccctgga acttcgagga     1440
agtcgtggac aagggtgcct ctgcacagtc cttcatcgag cgaatgacca acttcgacaa     1500
gaatctgccc aacgagaagg ttcttcccaa gcattcgctg ctctacgagt actttacagt     1560
ctacaacgaa ctcaccaaag tcaagtacgt taccgaggga atgcgaaagc ctgccttctt     1620
gtctggcgaa cagaagaaag ccattgtcga tctcctgttc aagaccaacc gaaaggtcac     1680
tgttaagcag ctcaaggagg actacttcaa gaaaatcgag tgtttcgaca gcgtcgagat     1740
ttccggagtt gaggaccgat tcaacgcctc tttgggcacc tatcacgatc tgctcaagat     1800
tatcaaggac aaggattttc tcgacaacga ggaaaacgag gacattctgg aggacatcgt     1860
gctcactctt accctgttcg aagatcggga gatgatcgag gaacgactca agacatacgc     1920
tcacctgttc gacgacaagg tcatgaaaca actcaagcga cgtagataca ccggctgggg     1980
aagactttcg cgaaagctca tcaacggcat cagagacaag cagtccggaa agaccattct     2040
ggactttctc aagtccgatg gctttgccaa ccgaaacttc atgcagctca ttcacgacga     2100
ttctcttacc ttcaaggagg acatccagaa ggcacaagtg tccggtcagg gcgacagctt     2160
gcacgaacat attgccaacc tggctggttc gccagccatc aagaaaggca ttctccagac     2220
tgtcaaggtt gtcgacgagc tggtgaaggt catgggacgt cacaagcccg agaacattgt     2280
gatcgagatg gccagagaga accagacaac tcaaaagggt cagaaaaact cgcgagagcg     2340
gatgaagcga atcgaggaag gcatcaagga gctgggatcc cagattctca aggagcatcc     2400
cgtcgagaac actcaactgc agaacgagaa gctgtatctc tactatctgc agaatggtcg     2460
agacatgtac gtggatcagg aactggacat caatcgtctc agcgactacg atgtggacca     2520
cattgtccct caatcctttc tcaaggacga ttctatcgac aacaaggtcc ttacacgatc     2580
cgacaagaac agaggcaagt cggacaacgt tcccagcgaa gaggtggtca aaaagatgaa     2640
gaactactgg cgacagctgc tcaacgccaa gctcattacc cagcgaaagt tcgacaatct     2700
taccaaggcc gagcgaggcg gtctgtccga gctcgacaag gctggcttca tcaagcgtca     2760
actcgtcgag accagacaga tcacaaagca cgtcgcacag attctcgatt ctcggatgaa     2820
caccaagtac gacgagaacg acaagctcat ccgagaggtc aaggtgatta ctctcaagtc     2880
caaactggtc tccgatttcc gaaaggactt tcagttctac aaggtgcgag agatcaacaa     2940
ttaccaccat gcccacgatg cttacctcaa cgccgtcgtt ggcactgcgc tcatcaagaa     3000
ataccccaag ctcgaaagcg agttcgttta cggcgattac aaggtctacg acgttcgaaa     3060
gatgattgcc aagtccgaac aggagattgg caaggctact gccaagtact tcttttactc     3120
caacatcatg aactttttca agaccgagat caccttggcc aacggagaga ttcgaaagag     3180
accacttatc gagaccaacg gcgaaactgg agagatcgtg tgggacaagg gtcgagactt     3240
tgcaaccgtg cgaaaggttc tgtcgatgcc tcaggtcaac atcgtcaaga aaaccgaggt     3300
tcagactggc ggattctcca aggagtcgat tctgcccaag cgaaactccg acaagctcat     3360
cgctcgaaag aaagactggg atcccaagaa atacggtggc ttcgattctc ctaccgtcgc     3420
ctattccgtg cttgtcgttg cgaaggtcga gaagggcaag tccaaaaagc tcaagtccgt     3480
caaggagctg ctcggaatta ccatcatgga gcgatcgagc ttcgagaaga atcccatcga     3540
cttcttggaa gccaagggtt acaaggaggt caagaaagac ctcattatca agctgcccaa     3600
gtactctctg ttcgaactgg agaacggtcg aaagcgtatg ctcgcctccg ctggcgagct     3660
gcagaaggga aacgagcttg ccttgccttc gaagtacgtc aactttctct atctggcttc     3720
tcactacgag aagctcaagg gttctcccga ggacaacgaa cagaagcaac tcttcgttga     3780
gcagcacaaa cattacctcg acgagattat cgagcagatt tccgagtttt cgaagcgagt     3840
catcctggct gatgccaact tggacaaggt gctctctgcc tacaacaagc atcgggacaa     3900
acccattcga gaacaggcgg agaacatcat tcacctgttt actcttacca acctgggtgc     3960
tcctgcagct ttcaagtact tcgataccac tatcgaccga aagcggtaca catccaccaa     4020
ggaggttctc gatgccaccc tgattcacca gtccatcact ggcctgtacg agacccgaat     4080
cgacctgtct cagcttggtg gcgactccag agccgatccc aagaaaaagc gaaaggtcta     4140
agcggccgca agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc     4200
caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc     4260
aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc aagtacaata     4320
ctaaacatac tgtacatact catactcgta cccgggcaac ggtttcactt gagtgcagtg     4380
gctagtgctc ttactcgtac agtgtgcaat actgcgtatc atagtctttg atgtatatcg     4440
tattcattca tgttagttgc gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct     4500
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     4560
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta     4620
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     4680
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     4740
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     4800
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     4860
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     4920
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     4980
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     5040
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     5100
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     5160
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     5220
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     5280
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     5340
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     5400
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     5460
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     5520
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     5580
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     5640
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     5700
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     5760
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     5820
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     5880
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     5940
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     6000
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     6060
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     6120
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     6180
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     6240
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     6300
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     6360
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     6420
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     6480
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     6540
ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     6600
tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     6660
tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     6720
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     6780
gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     6840
agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     6900
cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     6960
agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca     7020
ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     7080
acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     7140
ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg     7200
cgaattgggt accgggcccc ccctcgaggt cgatggtgtc gataagcttg atatcgaatt     7260
catgtcacac aaaccgatct tcgcctcaag gaaacctaat tctacatccg agagactgcc     7320
gagatccagt ctacactgat taattttcgg gccaataatt taaaaaaatc gtgttatata     7380
atattatatg tattatatat atacatcatg atgatactga cagtcatgtc ccattgctaa     7440
atagacagac tccatctgcc gcctccaact gatgttctca atatttaagg ggtcatctcg     7500
cattgtttaa taataaacag actccatcta ccgcctccaa atgatgttct caaaatatat     7560
tgtatgaact tatttttatt acttagtatt attagacaac ttacttgctt tatgaaaaac     7620
acttcctatt taggaaacaa tttataatgg cagttcgttc atttaacaat ttatgtagaa     7680
taaatgttat aaatgcgtat gggaaatctt aaatatggat agcataaatg atatctgcat     7740
tgcctaattc gaaatcaaca gcaacgaaaa aaatcccttg tacaacataa atagtcatcg     7800
agaaatatca actatcaaag aacagctatt cacacgttac tattgagatt attattggac     7860
gagaatcaca cactcaactg tctttctctc ttctagaaat acaggtacaa gtatgtacta     7920
ttctcattgt tcatacttct agtcatttca tcccacatat tccttggatt tctctccaat     7980
gaatgacatt ctatcttgca aattcaacaa ttataataag atataccaaa gtagcggtat     8040
agtggcaatc aaaaagcttc tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc     8100
attaaaggta tatatttatt tcttgttata taatcctttt gtttattaca tgggctggat     8160
acataaaggt attttgattt aattttttgc ttaaattcaa tcccccctcg ttcagtgtca     8220
actgtaatgg taggaaatta ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa     8280
aatcgtattt ccaggttaga cgttccgcag aatctagaat gcggtatgcg gtacattgtt     8340
cttcgaacgt aaaagttgcg ctccctgaga tattgtacat ttttgctttt acaagtacaa     8400
gtacatcgta caactatgta ctactgttga tgcatccaca acagtttgtt ttgttttttt     8460
ttgttttttt tttttctaat gattcattac cgctatgtat acctacttgt acttgtagta     8520
agccgggtta ttggcgttca attaatcata gacttatgaa tctgcacggt gtgcgctgcg     8580
agttactttt agcttatgca tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa     8640
cggatgctca atcgatttcg acagtaatta attaagtcat acacaagtca gctttcttcg     8700
agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     8760
agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     8820
atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     8880
ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     8940
atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     9000
cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     9060
tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     9120
ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     9180
accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     9240
gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     9300
ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     9360
ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     9420
ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     9480
tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     9540
tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     9600
atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     9660
agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     9720
agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     9780
ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     9840
accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     9900
agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     9960
gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg    10020
atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa    10080
gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc    10140
aatgacgagt cagacagata ctcgtcgacg tttaaaccat catctaaggg cctcaaaact    10200
acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10260
caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10320
aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10380
cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10440
gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10500
tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10560
accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10620
tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    10680
ttcgaaatct aaactacaca tcacac                                         10706

<210>  10
<211>  4140
<212>  DNA
<213>  Artificial sequence

<220>
<223>   Aar1- Cas9 ORF

<400>  10
atggacaaga aatactccat cggcctggac attggaacca actctgtcgg ctgggctgtc       60
atcaccgacg agtacaaggt gccctccaag aaattcaagg tcctcggaaa caccgatcga      120
cactccatca agaaaaacct cattggtgcc ctgttgttcg attctggcga gactgccgaa      180
gctaccagac tcaagcgaac tgctcggcga cgttacaccc gacggaagaa ccgaatctgc      240
tacctgcagg agatcttttc caacgagatg gccaaggtgg acgattcgtt ctttcatcga      300
ctggaggaat ccttcctcgt cgaggaagac aagaaacacg agcgtcatcc catctttggc      360
aacattgtgg acgaggttgc ttaccacgag aagtatccta ccatctacca tctccgaaag      420
aaactcgtcg attccaccga caaggcggat ctcagactta tctacctcgc tctggcacac      480
atgatcaagt ttcgaggtca tttcctcatc gagggcgatc tcaatcccga caacagcgat      540
gtggacaagc tgttcattca gctcgttcag acctacaacc agctgttcga ggaaaacccc      600
atcaatgcct ccggagtcga tgcaaaggcc atcttgtctg ctcgactctc gaagagcaga      660
cgactggaga acctcattgc ccaacttcct ggcgagaaaa agaacggact gtttggcaac      720
ctcattgccc tttctcttgg tctcacaccc aacttcaagt ccaacttcga tctggcggag      780
gacgccaagc tccagctgtc caaggacacc tacgacgatg acctcgacaa cctgcttgca      840
cagattggcg atcagtacgc cgacctgttt ctcgctgcca agaacctttc ggatgctatt      900
ctcttgtctg acattctgcg agtcaacacc gagatcacaa aggctcccct ttctgcctcc      960
atgatcaagc gatacgacga gcaccatcag gatctcacac tgctcaaggc tcttgtccga     1020
cagcaactgc ccgagaagta caaggagatc tttttcgatc agtcgaagaa cggctacgct     1080
ggatacatcg acggcggagc ctctcaggaa gagttctaca agttcatcaa gccaattctc     1140
gagaagatgg acggaaccga ggaactgctt gtcaagctca atcgagagga tctgcttcgg     1200
aagcaacgaa ccttcgacaa cggcagcatt cctcatcaga tccacctcgg tgagctgcac     1260
gccattcttc gacgtcagga agacttctac ccctttctca aggacaaccg agagaagatc     1320
gagaagattc ttacctttcg aatcccctac tatgttggtc ctcttgccag aggaaactct     1380
cgatttgctt ggatgactcg aaagtccgag gaaaccatca ctccctggaa cttcgaggaa     1440
gtcgtggaca agggtgcctc tgcacagtcc ttcatcgagc gaatgaccaa cttcgacaag     1500
aatctgccca acgagaaggt tcttcccaag cattcgctgc tctacgagta ctttacagtc     1560
tacaacgaac tcaccaaagt caagtacgtt accgagggaa tgcgaaagcc tgccttcttg     1620
tctggcgaac agaagaaagc cattgtcgat ctcctgttca agaccaaccg aaaggtcact     1680
gttaagcagc tcaaggagga ctacttcaag aaaatcgagt gtttcgacag cgtcgagatt     1740
tccggagttg aggaccgatt caacgcctct ttgggcacct atcacgatct gctcaagatt     1800
atcaaggaca aggattttct cgacaacgag gaaaacgagg acattctgga ggacatcgtg     1860
ctcactctta ccctgttcga agatcgggag atgatcgagg aacgactcaa gacatacgct     1920
cacctgttcg acgacaaggt catgaaacaa ctcaagcgac gtagatacac cggctgggga     1980
agactttcgc gaaagctcat caacggcatc agagacaagc agtccggaaa gaccattctg     2040
gactttctca agtccgatgg ctttgccaac cgaaacttca tgcagctcat tcacgacgat     2100
tctcttacct tcaaggagga catccagaag gcacaagtgt ccggtcaggg cgacagcttg     2160
cacgaacata ttgccaacct ggctggttcg ccagccatca agaaaggcat tctccagact     2220
gtcaaggttg tcgacgagct ggtgaaggtc atgggacgtc acaagcccga gaacattgtg     2280
atcgagatgg ccagagagaa ccagacaact caaaagggtc agaaaaactc gcgagagcgg     2340
atgaagcgaa tcgaggaagg catcaaggag ctgggatccc agattctcaa ggagcatccc     2400
gtcgagaaca ctcaactgca gaacgagaag ctgtatctct actatctgca gaatggtcga     2460
gacatgtacg tggatcagga actggacatc aatcgtctca gcgactacga tgtggaccac     2520
attgtccctc aatcctttct caaggacgat tctatcgaca acaaggtcct tacacgatcc     2580
gacaagaaca gaggcaagtc ggacaacgtt cccagcgaag aggtggtcaa aaagatgaag     2640
aactactggc gacagctgct caacgccaag ctcattaccc agcgaaagtt cgacaatctt     2700
accaaggccg agcgaggcgg tctgtccgag ctcgacaagg ctggcttcat caagcgtcaa     2760
ctcgtcgaga ccagacagat cacaaagcac gtcgcacaga ttctcgattc tcggatgaac     2820
accaagtacg acgagaacga caagctcatc cgagaggtca aggtgattac tctcaagtcc     2880
aaactggtct ccgatttccg aaaggacttt cagttctaca aggtgcgaga gatcaacaat     2940
taccaccatg cccacgatgc ttacctcaac gccgtcgttg gcactgcgct catcaagaaa     3000
taccccaagc tcgaaagcga gttcgtttac ggcgattaca aggtctacga cgttcgaaag     3060
atgattgcca agtccgaaca ggagattggc aaggctactg ccaagtactt cttttactcc     3120
aacatcatga actttttcaa gaccgagatc accttggcca acggagagat tcgaaagaga     3180
ccacttatcg agaccaacgg cgaaactgga gagatcgtgt gggacaaggg tcgagacttt     3240
gcaaccgtgc gaaaggttct gtcgatgcct caggtcaaca tcgtcaagaa aaccgaggtt     3300
cagactggcg gattctccaa ggagtcgatt ctgcccaagc gaaactccga caagctcatc     3360
gctcgaaaga aagactggga tcccaagaaa tacggtggct tcgattctcc taccgtcgcc     3420
tattccgtgc ttgtcgttgc gaaggtcgag aagggcaagt ccaaaaagct caagtccgtc     3480
aaggagctgc tcggaattac catcatggag cgatcgagct tcgagaagaa tcccatcgac     3540
ttcttggaag ccaagggtta caaggaggtc aagaaagacc tcattatcaa gctgcccaag     3600
tactctctgt tcgaactgga gaacggtcga aagcgtatgc tcgcctccgc tggcgagctg     3660
cagaagggaa acgagcttgc cttgccttcg aagtacgtca actttctcta tctggcttct     3720
cactacgaga agctcaaggg ttctcccgag gacaacgaac agaagcaact cttcgttgag     3780
cagcacaaac attacctcga cgagattatc gagcagattt ccgagttttc gaagcgagtc     3840
atcctggctg atgccaactt ggacaaggtg ctctctgcct acaacaagca tcgggacaaa     3900
cccattcgag aacaggcgga gaacatcatt cacctgttta ctcttaccaa cctgggtgct     3960
cctgcagctt tcaagtactt cgataccact atcgaccgaa agcggtacac atccaccaag     4020
gaggttctcg atgccaccct gattcaccag tccatcactg gcctgtacga gacccgaatc     4080
gacctgtctc agcttggtgg cgactccaga gccgatccca agaaaaagcg aaaggtctaa     4140

<210>  11
<211>  10706
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF141

<400>  11
catggacaag aaatactcca tcggcctgga cattggaacc aactctgtcg gctgggctgt       60
catcaccgac gagtacaagg tgccctccaa gaaattcaag gtcctcggaa acaccgatcg      120
acactccatc aagaaaaacc tcattggtgc cctgttgttc gattctggcg agactgccga      180
agctaccaga ctcaagcgaa ctgctcggcg acgttacacc cgacggaaga accgaatctg      240
ctacctgcag gagatctttt ccaacgagat ggccaaggtg gacgattcgt tctttcatcg      300
actggaggaa tccttcctcg tcgaggaaga caagaaacac gagcgtcatc ccatctttgg      360
caacattgtg gacgaggttg cttaccacga gaagtatcct accatctacc atctccgaaa      420
gaaactcgtc gattccaccg acaaggcgga tctcagactt atctacctcg ctctggcaca      480
catgatcaag tttcgaggtc atttcctcat cgagggcgat ctcaatcccg acaacagcga      540
tgtggacaag ctgttcattc agctcgttca gacctacaac cagctgttcg aggaaaaccc      600
catcaatgcc tccggagtcg atgcaaaggc catcttgtct gctcgactct cgaagagcag      660
acgactggag aacctcattg cccaacttcc tggcgagaaa aagaacggac tgtttggcaa      720
cctcattgcc ctttctcttg gtctcacacc caacttcaag tccaacttcg atctggcgga      780
ggacgccaag ctccagctgt ccaaggacac ctacgacgat gacctcgaca acctgcttgc      840
acagattggc gatcagtacg ccgacctgtt tctcgctgcc aagaaccttt cggatgctat      900
tctcttgtct gacattctgc gagtcaacac cgagatcaca aaggctcccc tttctgcctc      960
catgatcaag cgatacgacg agcaccatca ggatctcaca ctgctcaagg ctcttgtccg     1020
acagcaactg cccgagaagt acaaggagat ctttttcgat cagtcgaaga acggctacgc     1080
tggatacatc gacggcggag cctctcagga agagttctac aagttcatca agccaattct     1140
cgagaagatg gacggaaccg aggaactgct tgtcaagctc aatcgagagg atctgcttcg     1200
gaagcaacga accttcgaca acggcagcat tcctcatcag atccacctcg gtgagctgca     1260
cgccattctt cgacgtcagg aagacttcta cccctttctc aaggacaacc gagagaagat     1320
cgagaagatt cttacctttc gaatccccta ctatgttggt cctcttgcca gaggaaactc     1380
tcgatttgct tggatgactc gaaagtccga ggaaaccatc actccctgga acttcgagga     1440
agtcgtggac aagggtgcct ctgcacagtc cttcatcgag cgaatgacca acttcgacaa     1500
gaatctgccc aacgagaagg ttcttcccaa gcattcgctg ctctacgagt actttacagt     1560
ctacaacgaa ctcaccaaag tcaagtacgt taccgaggga atgcgaaagc ctgccttctt     1620
gtctggcgaa cagaagaaag ccattgtcga tctcctgttc aagaccaacc gaaaggtcac     1680
tgttaagcag ctcaaggagg actacttcaa gaaaatcgag tgtttcgaca gcgtcgagat     1740
ttccggagtt gaggaccgat tcaacgcctc tttgggcacc tatcacgatc tgctcaagat     1800
tatcaaggac aaggattttc tcgacaacga ggaaaacgag gacattctgg aggacatcgt     1860
gctcactctt accctgttcg aagatcggga gatgatcgag gaacgactca agacatacgc     1920
tcacctgttc gacgacaagg tcatgaaaca actcaagcga cgtagataca ccggctgggg     1980
aagactttcg cgaaagctca tcaacggcat cagagacaag cagtccggaa agaccattct     2040
ggactttctc aagtccgatg gctttgccaa ccgaaacttc atgcagctca ttcacgacga     2100
ttctcttacc ttcaaggagg acatccagaa ggcacaagtg tccggtcagg gcgacagctt     2160
gcacgaacat attgccaacc tggctggttc gccagccatc aagaaaggca ttctccagac     2220
tgtcaaggtt gtcgacgagc tggtgaaggt catgggacgt cacaagcccg agaacattgt     2280
gatcgagatg gccagagaga accagacaac tcaaaagggt cagaaaaact cgcgagagcg     2340
gatgaagcga atcgaggaag gcatcaagga gctgggatcc cagattctca aggagcatcc     2400
cgtcgagaac actcaactgc agaacgagaa gctgtatctc tactatctgc agaatggtcg     2460
agacatgtac gtggatcagg aactggacat caatcgtctc agcgactacg atgtggacca     2520
cattgtccct caatcctttc tcaaggacga ttctatcgac aacaaggtcc ttacacgatc     2580
cgacaagaac agaggcaagt cggacaacgt tcccagcgaa gaggtggtca aaaagatgaa     2640
gaactactgg cgacagctgc tcaacgccaa gctcattacc cagcgaaagt tcgacaatct     2700
taccaaggcc gagcgaggcg gtctgtccga gctcgacaag gctggcttca tcaagcgtca     2760
actcgtcgag accagacaga tcacaaagca cgtcgcacag attctcgatt ctcggatgaa     2820
caccaagtac gacgagaacg acaagctcat ccgagaggtc aaggtgatta ctctcaagtc     2880
caaactggtc tccgatttcc gaaaggactt tcagttctac aaggtgcgag agatcaacaa     2940
ttaccaccat gcccacgatg cttacctcaa cgccgtcgtt ggcactgcgc tcatcaagaa     3000
ataccccaag ctcgaaagcg agttcgttta cggcgattac aaggtctacg acgttcgaaa     3060
gatgattgcc aagtccgaac aggagattgg caaggctact gccaagtact tcttttactc     3120
caacatcatg aactttttca agaccgagat caccttggcc aacggagaga ttcgaaagag     3180
accacttatc gagaccaacg gcgaaactgg agagatcgtg tgggacaagg gtcgagactt     3240
tgcaaccgtg cgaaaggttc tgtcgatgcc tcaggtcaac atcgtcaaga aaaccgaggt     3300
tcagactggc ggattctcca aggagtcgat tctgcccaag cgaaactccg acaagctcat     3360
cgctcgaaag aaagactggg atcccaagaa atacggtggc ttcgattctc ctaccgtcgc     3420
ctattccgtg cttgtcgttg cgaaggtcga gaagggcaag tccaaaaagc tcaagtccgt     3480
caaggagctg ctcggaatta ccatcatgga gcgatcgagc ttcgagaaga atcccatcga     3540
cttcttggaa gccaagggtt acaaggaggt caagaaagac ctcattatca agctgcccaa     3600
gtactctctg ttcgaactgg agaacggtcg aaagcgtatg ctcgcctccg ctggcgagct     3660
gcagaaggga aacgagcttg ccttgccttc gaagtacgtc aactttctct atctggcttc     3720
tcactacgag aagctcaagg gttctcccga ggacaacgaa cagaagcaac tcttcgttga     3780
gcagcacaaa cattacctcg acgagattat cgagcagatt tccgagtttt cgaagcgagt     3840
catcctggct gatgccaact tggacaaggt gctctctgcc tacaacaagc atcgggacaa     3900
acccattcga gaacaggcgg agaacatcat tcacctgttt actcttacca acctgggtgc     3960
tcctgcagct ttcaagtact tcgataccac tatcgaccga aagcggtaca catccaccaa     4020
ggaggttctc gatgccaccc tgattcacca gtccatcact ggcctgtacg agacccgaat     4080
cgacctgtct cagcttggtg gcgactccag agccgatccc aagaaaaagc gaaaggtcta     4140
agcggccgca agtgtggatg gggaagtgag tgcccggttc tgtgtgcaca attggcaatc     4200
caagatggat ggattcaaca cagggatata gcgagctacg tggtggtgcg aggatatagc     4260
aacggatatt tatgtttgac acttgagaat gtacgataca agcactgtcc aagtacaata     4320
ctaaacatac tgtacatact catactcgta cccgggcaac ggtttcactt gagtgcagtg     4380
gctagtgctc ttactcgtac agtgtgcaat actgcgtatc atagtctttg atgtatatcg     4440
tattcattca tgttagttgc gtacgagccg gaagcataaa gtgtaaagcc tggggtgcct     4500
aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     4560
acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta     4620
ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc     4680
gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg     4740
caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     4800
tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     4860
gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     4920
ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     4980
cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     5040
tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     5100
tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     5160
cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     5220
agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     5280
agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg     5340
gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag     5400
aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag     5460
ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat     5520
gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct     5580
taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac     5640
tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa     5700
tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg     5760
gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt     5820
gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca     5880
ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt     5940
cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct     6000
tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     6060
cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     6120
agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     6180
cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     6240
aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     6300
aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     6360
gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     6420
gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     6480
tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     6540
ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     6600
tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     6660
tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     6720
tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     6780
gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     6840
agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     6900
cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     6960
agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca     7020
ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     7080
acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     7140
ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg     7200
cgaattgggt accgggcccc ccctcgaggt cgatggtgtc gataagcttg atatcgaatt     7260
catgtcacac aaaccgatct tcgcctcaag gaaacctaat tctacatccg agagactgcc     7320
gagatccagt ctacactgat taattttcgg gccaataatt taaaaaaatc gtgttatata     7380
atattatatg tattatatat atacatcatg atgatactga cagtcatgtc ccattgctaa     7440
atagacagac tccatctgcc gcctccaact gatgttctca atatttaagg ggtcatctcg     7500
cattgtttaa taataaacag actccatcta ccgcctccaa atgatgttct caaaatatat     7560
tgtatgaact tatttttatt acttagtatt attagacaac ttacttgctt tatgaaaaac     7620
acttcctatt taggaaacaa tttataatgg cagttcgttc atttaacaat ttatgtagaa     7680
taaatgttat aaatgcgtat gggaaatctt aaatatggat agcataaatg atatctgcat     7740
tgcctaattc gaaatcaaca gcaacgaaaa aaatcccttg tacaacataa atagtcatcg     7800
agaaatatca actatcaaag aacagctatt cacacgttac tattgagatt attattggac     7860
gagaatcaca cactcaactg tctttctctc ttctagaaat acaggtacaa gtatgtacta     7920
ttctcattgt tcatacttct agtcatttca tcccacatat tccttggatt tctctccaat     7980
gaatgacatt ctatcttgca aattcaacaa ttataataag atataccaaa gtagcggtat     8040
agtggcaatc aaaaagcttc tctggtgtgc ttctcgtatt tatttttatt ctaatgatcc     8100
attaaaggta tatatttatt tcttgttata taatcctttt gtttattaca tgggctggat     8160
acataaaggt attttgattt aattttttgc ttaaattcaa tcccccctcg ttcagtgtca     8220
actgtaatgg taggaaatta ccatactttt gaagaagcaa aaaaaatgaa agaaaaaaaa     8280
aatcgtattt ccaggttaga cgttccgcag aatctagaat gcggtatgcg gtacattgtt     8340
cttcgaacgt aaaagttgcg ctccctgaga tattgtacat ttttgctttt acaagtacaa     8400
gtacatcgta caactatgta ctactgttga tgcatccaca acagtttgtt ttgttttttt     8460
ttgttttttt tttttctaat gattcattac cgctatgtat acctacttgt acttgtagta     8520
agccgggtta ttggcgttca attaatcata gacttatgaa tctgcacggt gtgcgctgcg     8580
agttactttt agcttatgca tgctacttgg gtgtaatatt gggatctgtt cggaaatcaa     8640
cggatgctca atcgatttcg acagtaatta attaagtcat acacaagtca gctttcttcg     8700
agcctcatat aagtataagt agttcaacgt attagcactg tacccagcat ctccgtatcg     8760
agaaacacaa caacatgccc cattggacag atcatgcgga tacacaggtt gtgcagtatc     8820
atacatactc gatcagacag gtcgtctgac catcatacaa gctgaacaag cgctccatac     8880
ttgcacgctc tctatataca cagttaaatt acatatccat agtctaacct ctaacagtta     8940
atcttctggt aagcctccca gccagccttc tggtatcgct tggcctcctc aataggatct     9000
cggttctggc cgtacagacc tcggccgaca attatgatat ccgttccggt agacatgaca     9060
tcctcaacag ttcggtactg ctgtccgaga gcgtctccct tgtcgtcaag acccaccccg     9120
ggggtcagaa taagccagtc ctcagagtcg cccttaggtc ggttctgggc aatgaagcca     9180
accacaaact cggggtcgga tcgggcaagc tcaatggtct gcttggagta ctcgccagtg     9240
gccagagagc ccttgcaaga cagctcggcc agcatgagca gacctctggc cagcttctcg     9300
ttgggagagg ggactaggaa ctccttgtac tgggagttct cgtagtcaga gacgtcctcc     9360
ttcttctgtt cagagacagt ttcctcggca ccagctcgca ggccagcaat gattccggtt     9420
ccgggtacac cgtgggcgtt ggtgatatcg gaccactcgg cgattcggtg acaccggtac     9480
tggtgcttga cagtgttgcc aatatctgcg aactttctgt cctcgaacag gaagaaaccg     9540
tgcttaagag caagttcctt gagggggagc acagtgccgg cgtaggtgaa gtcgtcaatg     9600
atgtcgatat gggttttgat catgcacaca taaggtccga ccttatcggc aagctcaatg     9660
agctccttgg tggtggtaac atccagagaa gcacacaggt tggttttctt ggctgccacg     9720
agcttgagca ctcgagcggc aaaggcggac ttgtggacgt tagctcgagc ttcgtaggag     9780
ggcattttgg tggtgaagag gagactgaaa taaatttagt ctgcagaact ttttatcgga     9840
accttatctg gggcagtgaa gtatatgtta tggtaatagt tacgagttag ttgaacttat     9900
agatagactg gactatacgg ctatcggtcc aaattagaaa gaacgtcaat ggctctctgg     9960
gcgtcgcctt tgccgacaaa aatgtgatca tgatgaaagc cagcaatgac gttgcagctg    10020
atattgttgt cggccaaccg cgccgaaaac gcagctgtca gacccacagc ctccaacgaa    10080
gaatgtatcg tcaaagtgat ccaagcacac tcatagttgg agtcgtactc caaaggcggc    10140
aatgacgagt cagacagata ctcgtcgacg tttaaaccat catctaaggg cctcaaaact    10200
acctcggaac tgctgcgctg atctggacac cacagaggtt ccgagcactt taggttgcac    10260
caaatgtccc accaggtgca ggcagaaaac gctggaacag cgtgtacagt ttgtcttaac    10320
aaaaagtgag ggcgctgagg tcgagcaggg tggtgtgact tgttatagcc tttagagctg    10380
cgaaagcgcg tatggatttg gctcatcagg ccagattgag ggtctgtgga cacatgtcat    10440
gttagtgtac ttcaatcgcc ccctggatat agccccgaca ataggccgtg gcctcatttt    10500
tttgccttcc gcacatttcc attgctcggt acccacacct tgcttctcct gcacttgcca    10560
accttaatac tggtttacat tgaccaacat cttacaagcg gggggcttgt ctagggtata    10620
tataaacagt ggctctccca atcggttgcc agtctctttt ttcctttctt tccccacaga    10680
ttcgaaatct aaactacaca tcacac                                         10706

<210>  12
<211>  1048
<212>  DNA
<213>  Artificial sequence

<220>
<223>  high-throughput cloning cassette

<400>  12
gcgcacgtta attaaatttt ttttgatttt cttttttgac cccgtcttca attacacttc       60
ccaactggga acacccctct ttatcgaccc attttaggta atttacccta gcccattgtc      120
tccataagga atattaccct aacccacagt ccagggtgcc caggtccttc tttggccaaa      180
ttttaacttc ggtcctatgg cacagcggta gcgcgtgaga ttgcaaatct taaggtcccg      240
agttcgaatc tcggtgggac ctagttattt ttgatagata atttcgtgat gattagaaac      300
ttaacgcaaa ataatggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca      360
tgcttcggca tggcgaatgg gacgcaggtg atggcgggat cgttgtatat ttcttgacac      420
cttttcggca tcgccctaaa ttcggcgtcc tcatattgtg tgaggacgtt ttattacgtg      480
tttacgaagc aaaagctaaa accaggagct atttaatggc aacagttaac cagctggtac      540
gcaaaccacg tgctcgcaaa gttgcgaaaa gcaacgtgcc tgcgctggaa gcatgcccgc      600
aaaaacgtgg cgtatgtact cgtgtatata ctaccactcc taaaaaaccg aactccgcgc      660
tgcgtaaagt atgccgtgtt cgtctgacta acggtttcga agtgacttcc tacatcggtg      720
gtgaaggtca caacctgcag gagcactccg tgatcctgat ccgtggcggt cgtgttaaag      780
acctcccggg tgttcgttac cacaccgtac gtggtgcgct tgactgctcc ggcgttaaag      840
accgtaagca ggctcgttcc aagtatggcg tgaagcgtcc taaggcttag gttaataaca      900
ggcctgctgg taatcgcagg cctttttatt tttacacctg cgttttagag ctagaaatag      960
caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt     1020
tttttttgtt ttttatcgat gcgcgcac                                        1048

<210>  13
<211>  300
<212>  DNA
<213>  Yarrowia lipolytica

<400>  13
attttttttg attttctttt ttgaccccgt cttcaattac acttcccaac tgggaacacc       60
cctctttatc gacccatttt aggtaattta ccctagccca ttgtctccat aaggaatatt      120
accctaaccc acagtccagg gtgcccaggt ccttctttgg ccaaatttta acttcggtcc      180
tatggcacag cggtagcgcg tgagattgca aatcttaagg tcccgagttc gaatctcggt      240
gggacctagt tatttttgat agataatttc gtgatgatta gaaacttaac gcaaaataat      300

<210>  14
<211>  68
<212>  DNA
<213>  Herpes Delta virus

<400>  14
ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg       60
aatgggac                                                                68

<210>  15
<211>  544
<212>  DNA
<213>  Escherischia coli

<400>  15
atggcgggat cgttgtatat ttcttgacac cttttcggca tcgccctaaa ttcggcgtcc       60
tcatattgtg tgaggacgtt ttattacgtg tttacgaagc aaaagctaaa accaggagct      120
atttaatggc aacagttaac cagctggtac gcaaaccacg tgctcgcaaa gttgcgaaaa      180
gcaacgtgcc tgcgctggaa gcatgcccgc aaaaacgtgg cgtatgtact cgtgtatata      240
ctaccactcc taaaaaaccg aactccgcgc tgcgtaaagt atgccgtgtt cgtctgacta      300
acggtttcga agtgacttcc tacatcggtg gtgaaggtca caacctgcag gagcactccg      360
tgatcctgat ccgtggcggt cgtgttaaag acctcccggg tgttcgttac cacaccgtac      420
gtggtgcgct tgactgctcc ggcgttaaag accgtaagca ggctcgttcc aagtatggcg      480
tgaagcgtcc taaggcttag gttaataaca ggcctgctgg taatcgcagg cctttttatt      540
ttta                                                                   544

<210>  16
<211>  80
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA encoding Cas9 CER domain

<400>  16
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60
ggcaccgagt cggtgctttt                                                   80

<210>  17
<211>  14
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  17
tttttttgtt tttt                                                         14

<210>  18
<211>  11714
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF291

<400>  18
cgataaaaaa caaaaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac       60
tagccttatt ttaacttgct atttctagct ctaaaacgca ggtgtaaaaa taaaaaggcc      120
tgcgattacc agcaggcctg ttattaacct aagccttagg acgcttcacg ccatacttgg      180
aacgagcctg cttacggtct ttaacgccgg agcagtcaag cgcaccacgt acggtgtggt      240
aacgaacacc cgggaggtct ttaacacgac cgccacggat caggatcacg gagtgctcct      300
gcaggttgtg accttcacca ccgatgtagg aagtcacttc gaaaccgtta gtcagacgaa      360
cacggcatac tttacgcagc gcggagttcg gttttttagg agtggtagta tatacacgag      420
tacatacgcc acgtttttgc gggcatgctt ccagcgcagg cacgttgctt ttcgcaactt      480
tgcgagcacg tggtttgcgt accagctggt taactgttgc cattaaatag ctcctggttt      540
tagcttttgc ttcgtaaaca cgtaataaaa cgtcctcaca caatatgagg acgccgaatt      600
tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca cctgcgtccc      660
attcgccatg ccgaagcatg ttgcccagcc ggcgccagcg aggaggctgg gaccatgccg      720
gccattattt tgcgttaagt ttctaatcat cacgaaatta tctatcaaaa ataactaggt      780
cccaccgaga ttcgaactcg ggaccttaag atttgcaatc tcacgcgcta ccgctgtgcc      840
ataggaccga agttaaaatt tggccaaaga aggacctggg caccctggac tgtgggttag      900
ggtaatattc cttatggaga caatgggcta gggtaaatta cctaaaatgg gtcgataaag      960
aggggtgttc ccagttggga agtgtaattg aagacggggt caaaaaagaa aatcaaaaaa     1020
aatttaatta agtcatacac aagtcagctt tcttcgagcc tcatataagt ataagtagtt     1080
caacgtatta gcactgtacc cagcatctcc gtatcgagaa acacaacaac atgccccatt     1140
ggacagatca tgcggataca caggttgtgc agtatcatac atactcgatc agacaggtcg     1200
tctgaccatc atacaagctg aacaagcgct ccatacttgc acgctctcta tatacacagt     1260
taaattacat atccatagtc taacctctaa cagttaatct tctggtaagc ctcccagcca     1320
gccttctggt atcgcttggc ctcctcaata ggatctcggt tctggccgta cagacctcgg     1380
ccgacaatta tgatatccgt tccggtagac atgacatcct caacagttcg gtactgctgt     1440
ccgagagcgt ctcccttgtc gtcaagaccc accccggggg tcagaataag ccagtcctca     1500
gagtcgccct taggtcggtt ctgggcaatg aagccaacca caaactcggg gtcggatcgg     1560
gcaagctcaa tggtctgctt ggagtactcg ccagtggcca gagagccctt gcaagacagc     1620
tcggccagca tgagcagacc tctggccagc ttctcgttgg gagaggggac taggaactcc     1680
ttgtactggg agttctcgta gtcagagacg tcctccttct tctgttcaga gacagtttcc     1740
tcggcaccag ctcgcaggcc agcaatgatt ccggttccgg gtacaccgtg ggcgttggtg     1800
atatcggacc actcggcgat tcggtgacac cggtactggt gcttgacagt gttgccaata     1860
tctgcgaact ttctgtcctc gaacaggaag aaaccgtgct taagagcaag ttccttgagg     1920
gggagcacag tgccggcgta ggtgaagtcg tcaatgatgt cgatatgggt tttgatcatg     1980
cacacataag gtccgacctt atcggcaagc tcaatgagct ccttggtggt ggtaacatcc     2040
agagaagcac acaggttggt tttcttggct gccacgagct tgagcactcg agcggcaaag     2100
gcggacttgt ggacgttagc tcgagcttcg taggagggca ttttggtggt gaagaggaga     2160
ctgaaataaa tttagtctgc agaacttttt atcggaacct tatctggggc agtgaagtat     2220
atgttatggt aatagttacg agttagttga acttatagat agactggact atacggctat     2280
cggtccaaat tagaaagaac gtcaatggct ctctgggcgt cgcctttgcc gacaaaaatg     2340
tgatcatgat gaaagccagc aatgacgttg cagctgatat tgttgtcggc caaccgcgcc     2400
gaaaacgcag ctgtcagacc cacagcctcc aacgaagaat gtatcgtcaa agtgatccaa     2460
gcacactcat agttggagtc gtactccaaa ggcggcaatg acgagtcaga cagatactcg     2520
tcgacgttta aaccatcatc taagggcctc aaaactacct cggaactgct gcgctgatct     2580
ggacaccaca gaggttccga gcactttagg ttgcaccaaa tgtcccacca ggtgcaggca     2640
gaaaacgctg gaacagcgtg tacagtttgt cttaacaaaa agtgagggcg ctgaggtcga     2700
gcagggtggt gtgacttgtt atagccttta gagctgcgaa agcgcgtatg gatttggctc     2760
atcaggccag attgagggtc tgtggacaca tgtcatgtta gtgtacttca atcgccccct     2820
ggatatagcc ccgacaatag gccgtggcct catttttttg ccttccgcac atttccattg     2880
ctcggtaccc acaccttgct tctcctgcac ttgccaacct taatactggt ttacattgac     2940
caacatctta caagcggggg gcttgtctag ggtatatata aacagtggct ctcccaatcg     3000
gttgccagtc tcttttttcc tttctttccc cacagattcg aaatctaaac tacacatcac     3060
accatggaca agaaatactc catcggcctg gacattggaa ccaactctgt cggctgggct     3120
gtcatcaccg acgagtacaa ggtgccctcc aagaaattca aggtcctcgg aaacaccgat     3180
cgacactcca tcaagaaaaa cctcattggt gccctgttgt tcgattctgg cgagactgcc     3240
gaagctacca gactcaagcg aactgctcgg cgacgttaca cccgacggaa gaaccgaatc     3300
tgctacctgc aggagatctt ttccaacgag atggccaagg tggacgattc gttctttcat     3360
cgactggagg aatccttcct cgtcgaggaa gacaagaaac acgagcgtca tcccatcttt     3420
ggcaacattg tggacgaggt tgcttaccac gagaagtatc ctaccatcta ccatctccga     3480
aagaaactcg tcgattccac cgacaaggcg gatctcagac ttatctacct cgctctggca     3540
cacatgatca agtttcgagg tcatttcctc atcgagggcg atctcaatcc cgacaacagc     3600
gatgtggaca agctgttcat tcagctcgtt cagacctaca accagctgtt cgaggaaaac     3660
cccatcaatg cctccggagt cgatgcaaag gccatcttgt ctgctcgact ctcgaagagc     3720
agacgactgg agaacctcat tgcccaactt cctggcgaga aaaagaacgg actgtttggc     3780
aacctcattg ccctttctct tggtctcaca cccaacttca agtccaactt cgatctggcg     3840
gaggacgcca agctccagct gtccaaggac acctacgacg atgacctcga caacctgctt     3900
gcacagattg gcgatcagta cgccgacctg tttctcgctg ccaagaacct ttcggatgct     3960
attctcttgt ctgacattct gcgagtcaac accgagatca caaaggctcc cctttctgcc     4020
tccatgatca agcgatacga cgagcaccat caggatctca cactgctcaa ggctcttgtc     4080
cgacagcaac tgcccgagaa gtacaaggag atctttttcg atcagtcgaa gaacggctac     4140
gctggataca tcgacggcgg agcctctcag gaagagttct acaagttcat caagccaatt     4200
ctcgagaaga tggacggaac cgaggaactg cttgtcaagc tcaatcgaga ggatctgctt     4260
cggaagcaac gaaccttcga caacggcagc attcctcatc agatccacct cggtgagctg     4320
cacgccattc ttcgacgtca ggaagacttc tacccctttc tcaaggacaa ccgagagaag     4380
atcgagaaga ttcttacctt tcgaatcccc tactatgttg gtcctcttgc cagaggaaac     4440
tctcgatttg cttggatgac tcgaaagtcc gaggaaacca tcactccctg gaacttcgag     4500
gaagtcgtgg acaagggtgc ctctgcacag tccttcatcg agcgaatgac caacttcgac     4560
aagaatctgc ccaacgagaa ggttcttccc aagcattcgc tgctctacga gtactttaca     4620
gtctacaacg aactcaccaa agtcaagtac gttaccgagg gaatgcgaaa gcctgccttc     4680
ttgtctggcg aacagaagaa agccattgtc gatctcctgt tcaagaccaa ccgaaaggtc     4740
actgttaagc agctcaagga ggactacttc aagaaaatcg agtgtttcga cagcgtcgag     4800
atttccggag ttgaggaccg attcaacgcc tctttgggca cctatcacga tctgctcaag     4860
attatcaagg acaaggattt tctcgacaac gaggaaaacg aggacattct ggaggacatc     4920
gtgctcactc ttaccctgtt cgaagatcgg gagatgatcg aggaacgact caagacatac     4980
gctcacctgt tcgacgacaa ggtcatgaaa caactcaagc gacgtagata caccggctgg     5040
ggaagacttt cgcgaaagct catcaacggc atcagagaca agcagtccgg aaagaccatt     5100
ctggactttc tcaagtccga tggctttgcc aaccgaaact tcatgcagct cattcacgac     5160
gattctctta ccttcaagga ggacatccag aaggcacaag tgtccggtca gggcgacagc     5220
ttgcacgaac atattgccaa cctggctggt tcgccagcca tcaagaaagg cattctccag     5280
actgtcaagg ttgtcgacga gctggtgaag gtcatgggac gtcacaagcc cgagaacatt     5340
gtgatcgaga tggccagaga gaaccagaca actcaaaagg gtcagaaaaa ctcgcgagag     5400
cggatgaagc gaatcgagga aggcatcaag gagctgggat cccagattct caaggagcat     5460
cccgtcgaga acactcaact gcagaacgag aagctgtatc tctactatct gcagaatggt     5520
cgagacatgt acgtggatca ggaactggac atcaatcgtc tcagcgacta cgatgtggac     5580
cacattgtcc ctcaatcctt tctcaaggac gattctatcg acaacaaggt ccttacacga     5640
tccgacaaga acagaggcaa gtcggacaac gttcccagcg aagaggtggt caaaaagatg     5700
aagaactact ggcgacagct gctcaacgcc aagctcatta cccagcgaaa gttcgacaat     5760
cttaccaagg ccgagcgagg cggtctgtcc gagctcgaca aggctggctt catcaagcgt     5820
caactcgtcg agaccagaca gatcacaaag cacgtcgcac agattctcga ttctcggatg     5880
aacaccaagt acgacgagaa cgacaagctc atccgagagg tcaaggtgat tactctcaag     5940
tccaaactgg tctccgattt ccgaaaggac tttcagttct acaaggtgcg agagatcaac     6000
aattaccacc atgcccacga tgcttacctc aacgccgtcg ttggcactgc gctcatcaag     6060
aaatacccca agctcgaaag cgagttcgtt tacggcgatt acaaggtcta cgacgttcga     6120
aagatgattg ccaagtccga acaggagatt ggcaaggcta ctgccaagta cttcttttac     6180
tccaacatca tgaacttttt caagaccgag atcaccttgg ccaacggaga gattcgaaag     6240
agaccactta tcgagaccaa cggcgaaact ggagagatcg tgtgggacaa gggtcgagac     6300
tttgcaaccg tgcgaaaggt tctgtcgatg cctcaggtca acatcgtcaa gaaaaccgag     6360
gttcagactg gcggattctc caaggagtcg attctgccca agcgaaactc cgacaagctc     6420
atcgctcgaa agaaagactg ggatcccaag aaatacggtg gcttcgattc tcctaccgtc     6480
gcctattccg tgcttgtcgt tgcgaaggtc gagaagggca agtccaaaaa gctcaagtcc     6540
gtcaaggagc tgctcggaat taccatcatg gagcgatcga gcttcgagaa gaatcccatc     6600
gacttcttgg aagccaaggg ttacaaggag gtcaagaaag acctcattat caagctgccc     6660
aagtactctc tgttcgaact ggagaacggt cgaaagcgta tgctcgcctc cgctggcgag     6720
ctgcagaagg gaaacgagct tgccttgcct tcgaagtacg tcaactttct ctatctggct     6780
tctcactacg agaagctcaa gggttctccc gaggacaacg aacagaagca actcttcgtt     6840
gagcagcaca aacattacct cgacgagatt atcgagcaga tttccgagtt ttcgaagcga     6900
gtcatcctgg ctgatgccaa cttggacaag gtgctctctg cctacaacaa gcatcgggac     6960
aaacccattc gagaacaggc ggagaacatc attcacctgt ttactcttac caacctgggt     7020
gctcctgcag ctttcaagta cttcgatacc actatcgacc gaaagcggta cacatccacc     7080
aaggaggttc tcgatgccac cctgattcac cagtccatca ctggcctgta cgagacccga     7140
atcgacctgt ctcagcttgg tggcgactcc agagccgatc ccaagaaaaa gcgaaaggtc     7200
taagcggccg caagtgtgga tggggaagtg agtgcccggt tctgtgtgca caattggcaa     7260
tccaagatgg atggattcaa cacagggata tagcgagcta cgtggtggtg cgaggatata     7320
gcaacggata tttatgtttg acacttgaga atgtacgata caagcactgt ccaagtacaa     7380
tactaaacat actgtacata ctcatactcg tacccgggca acggtttcac ttgagtgcag     7440
tggctagtgc tcttactcgt acagtgtgca atactgcgta tcatagtctt tgatgtatat     7500
cgtattcatt catgttagtt gcgtacgagc cggaagcata aagtgtaaag cctggggtgc     7560
ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     7620
aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     7680
tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     7740
gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     7800
cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     7860
gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     7920
aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     7980
ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     8040
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     8100
ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     8160
cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     8220
agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     8280
gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     8340
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     8400
tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     8460
agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     8520
agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     8580
atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     8640
cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     8700
actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     8760
aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     8820
cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     8880
ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     8940
cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     9000
ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     9060
cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     9120
ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     9180
tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     9240
ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     9300
aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     9360
gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     9420
gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     9480
ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     9540
catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     9600
atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt     9660
ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc     9720
tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg     9780
gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta     9840
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt     9900
ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat     9960
ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa    10020
tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc    10080
cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta    10140
ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg    10200
ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag    10260
ggcgaattgg gtaccgggcc ccccctcgag gtcgatggtg tcgataagct tgatatcgaa    10320
ttcatgtcac acaaaccgat cttcgcctca aggaaaccta attctacatc cgagagactg    10380
ccgagatcca gtctacactg attaattttc gggccaataa tttaaaaaaa tcgtgttata    10440
taatattata tgtattatat atatacatca tgatgatact gacagtcatg tcccattgct    10500
aaatagacag actccatctg ccgcctccaa ctgatgttct caatatttaa ggggtcatct    10560
cgcattgttt aataataaac agactccatc taccgcctcc aaatgatgtt ctcaaaatat    10620
attgtatgaa cttattttta ttacttagta ttattagaca acttacttgc tttatgaaaa    10680
acacttccta tttaggaaac aatttataat ggcagttcgt tcatttaaca atttatgtag    10740
aataaatgtt ataaatgcgt atgggaaatc ttaaatatgg atagcataaa tgatatctgc    10800
attgcctaat tcgaaatcaa cagcaacgaa aaaaatccct tgtacaacat aaatagtcat    10860
cgagaaatat caactatcaa agaacagcta ttcacacgtt actattgaga ttattattgg    10920
acgagaatca cacactcaac tgtctttctc tcttctagaa atacaggtac aagtatgtac    10980
tattctcatt gttcatactt ctagtcattt catcccacat attccttgga tttctctcca    11040
atgaatgaca ttctatcttg caaattcaac aattataata agatatacca aagtagcggt    11100
atagtggcaa tcaaaaagct tctctggtgt gcttctcgta tttattttta ttctaatgat    11160
ccattaaagg tatatattta tttcttgtta tataatcctt ttgtttatta catgggctgg    11220
atacataaag gtattttgat ttaatttttt gcttaaattc aatcccccct cgttcagtgt    11280
caactgtaat ggtaggaaat taccatactt ttgaagaagc aaaaaaaatg aaagaaaaaa    11340
aaaatcgtat ttccaggtta gacgttccgc agaatctaga atgcggtatg cggtacattg    11400
ttcttcgaac gtaaaagttg cgctccctga gatattgtac atttttgctt ttacaagtac    11460
aagtacatcg tacaactatg tactactgtt gatgcatcca caacagtttg ttttgttttt    11520
ttttgttttt tttttttcta atgattcatt accgctatgt atacctactt gtacttgtag    11580
taagccgggt tattggcgtt caattaatca tagacttatg aatctgcacg gtgtgcgctg    11640
cgagttactt ttagcttatg catgctactt gggtgtaata ttgggatctg ttcggaaatc    11700
aacggatgct caat                                                      11714

<210>  19
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>   Can1-1F

<400>  19
aatgggactc aaacgattac ccaccctcgt tt                                     32

<210>  20
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1-1R

<400>  20
tctaaaacga gggtgggtaa tcgtttgagt cc                                     32

<210>  21
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA encoding Can1-1 VT domain

<400>  21
tcaaacgatt acccaccctc                                                   20

<210>  22
<211>  23
<212>  DNA
<213>  Yarrowia lipolytica

<400>  22
tcaaacgatt acccaccctc cgg                                               23

<210>  23
<211>  1719
<212>  DNA
<213>  Yarrowia lipolytica

<400>  23
atggaaaaga cattttcaaa cgattaccca ccctccggga ctgaggccca catccacatc       60
aaccacacgg cccactcgga tgactcagag gaggtgccct cgcacaagga aaattacaac      120
accagtggcc acgacctgga ggagtccgac ccggataacc atgtcggtga gaccctcgag      180
gtcaagcgag gtctcaagat gcgacacatc tccatgatct cgcttggagg aaccattggt      240
accggtctct tcattggtac cggaggagct ctccagcagg ccggtccctg tggcgccctc      300
gtcgcctacg tgttcatggc caccattgtc tactctgttg ccgagtctct tggagaactg      360
gctacgtaca ttcccatcac cggctccttt gccgtcttta ctacccgata tctgtcacag      420
tcgtttggtg cctccatggg ctggctatac tggttctcgt gggcgatcac cttcgccatc      480
gagctcaaca ccattggtcc cgtgattgag tactggactg acgccgttcc tactgctgcc      540
tggattgcca tcttcttcgt catcctcact accatcaact tcttccccgt gggcttctat      600
ggcgaagtcg agttctgggt ggcctccgtg aaggtcattg ccatcattgg atggctcatc      660
tacgcgctct gcatgacgtg tggagcaggt gtaacaggtc ctgtgggatt cagatactgg      720
aaccaccccg gacccatggg agacggaatc tggaccgacg gcgtgcccat tgtgcgaaac      780
gcgcccggtc gacgattcat gggatggctc aattcgctcg ttaacgccgc cttcacctac      840
cagggctgtg agctggtcgg agtcactgcc ggtgaggccc agaaccccag aaagtccgtc      900
cctcgagcca tcaaccgagt ctttgctcga atttgcatct tctacattgg ctctatcttc      960
ttcatgggca tgctcgtgcc ctttaacgac cccaagctga ccgatgactc ctccgtcatc     1020
gcctcctctc cttttgttat tgccattatc aactctggca ccaaggtgct ccctcacatt     1080
ttcaacgccg tcattctcat caccctgatt tcggcaggaa actccaacgt ctacattggc     1140
tcgcgagtgg tctacgccct ggctgactcc ggaaccgcac caaagttctt caagcgaacc     1200
accaagaagg gagtgccgta cgtggcagtc tgcttcacct cggcgtttgg tctgctggcc     1260
ttcatgtctg tgtccgagtc gtcgtccact gtcttcgact ggttcatcaa catctccgct     1320
gtggccggcc tcatctgttg ggccttcatc tctgcctccc acatccgatt catgcaagtg     1380
cttaagcaca gagggatctc cagagatacg ctgcccttca aggcacgatg gcagccattc     1440
tactcatggt acgcgctcgt ctccatcatc ttcatcactc tcatccaggg cttcacgtcc     1500
ttctggcact ttaccgccgc caagttcatg actgcataca tctccgtcat tgtctgggtc     1560
ggtttgtaca ttatcttcca gtgtctgttc cgatgcaagt tccttatccc tattgaggat     1620
gtggacattg acaccggccg acgagagatt gacgacgatg tgtgggagga gaagatcccc     1680
acaaagtggt acgagaagtt ttggaatatt attgcataa                            1719

<210>  24
<211>  11176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   pRF303

<400>  24
tctaaaacga gggtgggtaa tcgtttgagt cccattcgcc atgccgaagc atgttgccca       60
gccggcgcca gcgaggaggc tgggaccatg ccggccatta ttttgcgtta agtttctaat      120
catcacgaaa ttatctatca aaaataacta ggtcccaccg agattcgaac tcgggacctt      180
aagatttgca atctcacgcg ctaccgctgt gccataggac cgaagttaaa atttggccaa      240
agaaggacct gggcaccctg gactgtgggt tagggtaata ttccttatgg agacaatggg      300
ctagggtaaa ttacctaaaa tgggtcgata aagaggggtg ttcccagttg ggaagtgtaa      360
ttgaagacgg ggtcaaaaaa gaaaatcaaa aaaaatttaa ttaagtcata cacaagtcag      420
ctttcttcga gcctcatata agtataagta gttcaacgta ttagcactgt acccagcatc      480
tccgtatcga gaaacacaac aacatgcccc attggacaga tcatgcggat acacaggttg      540
tgcagtatca tacatactcg atcagacagg tcgtctgacc atcatacaag ctgaacaagc      600
gctccatact tgcacgctct ctatatacac agttaaatta catatccata gtctaacctc      660
taacagttaa tcttctggta agcctcccag ccagccttct ggtatcgctt ggcctcctca      720
ataggatctc ggttctggcc gtacagacct cggccgacaa ttatgatatc cgttccggta      780
gacatgacat cctcaacagt tcggtactgc tgtccgagag cgtctccctt gtcgtcaaga      840
cccaccccgg gggtcagaat aagccagtcc tcagagtcgc ccttaggtcg gttctgggca      900
atgaagccaa ccacaaactc ggggtcggat cgggcaagct caatggtctg cttggagtac      960
tcgccagtgg ccagagagcc cttgcaagac agctcggcca gcatgagcag acctctggcc     1020
agcttctcgt tgggagaggg gactaggaac tccttgtact gggagttctc gtagtcagag     1080
acgtcctcct tcttctgttc agagacagtt tcctcggcac cagctcgcag gccagcaatg     1140
attccggttc cgggtacacc gtgggcgttg gtgatatcgg accactcggc gattcggtga     1200
caccggtact ggtgcttgac agtgttgcca atatctgcga actttctgtc ctcgaacagg     1260
aagaaaccgt gcttaagagc aagttccttg agggggagca cagtgccggc gtaggtgaag     1320
tcgtcaatga tgtcgatatg ggttttgatc atgcacacat aaggtccgac cttatcggca     1380
agctcaatga gctccttggt ggtggtaaca tccagagaag cacacaggtt ggttttcttg     1440
gctgccacga gcttgagcac tcgagcggca aaggcggact tgtggacgtt agctcgagct     1500
tcgtaggagg gcattttggt ggtgaagagg agactgaaat aaatttagtc tgcagaactt     1560
tttatcggaa ccttatctgg ggcagtgaag tatatgttat ggtaatagtt acgagttagt     1620
tgaacttata gatagactgg actatacggc tatcggtcca aattagaaag aacgtcaatg     1680
gctctctggg cgtcgccttt gccgacaaaa atgtgatcat gatgaaagcc agcaatgacg     1740
ttgcagctga tattgttgtc ggccaaccgc gccgaaaacg cagctgtcag acccacagcc     1800
tccaacgaag aatgtatcgt caaagtgatc caagcacact catagttgga gtcgtactcc     1860
aaaggcggca atgacgagtc agacagatac tcgtcgacgt ttaaaccatc atctaagggc     1920
ctcaaaacta cctcggaact gctgcgctga tctggacacc acagaggttc cgagcacttt     1980
aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt     2040
tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt ggtgtgactt gttatagcct     2100
ttagagctgc gaaagcgcgt atggatttgg ctcatcaggc cagattgagg gtctgtggac     2160
acatgtcatg ttagtgtact tcaatcgccc cctggatata gccccgacaa taggccgtgg     2220
cctcattttt ttgccttccg cacatttcca ttgctcggta cccacacctt gcttctcctg     2280
cacttgccaa ccttaatact ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc     2340
tagggtatat ataaacagtg gctctcccaa tcggttgcca gtctcttttt tcctttcttt     2400
ccccacagat tcgaaatcta aactacacat cacaccatgg acaagaaata ctccatcggc     2460
ctggacattg gaaccaactc tgtcggctgg gctgtcatca ccgacgagta caaggtgccc     2520
tccaagaaat tcaaggtcct cggaaacacc gatcgacact ccatcaagaa aaacctcatt     2580
ggtgccctgt tgttcgattc tggcgagact gccgaagcta ccagactcaa gcgaactgct     2640
cggcgacgtt acacccgacg gaagaaccga atctgctacc tgcaggagat cttttccaac     2700
gagatggcca aggtggacga ttcgttcttt catcgactgg aggaatcctt cctcgtcgag     2760
gaagacaaga aacacgagcg tcatcccatc tttggcaaca ttgtggacga ggttgcttac     2820
cacgagaagt atcctaccat ctaccatctc cgaaagaaac tcgtcgattc caccgacaag     2880
gcggatctca gacttatcta cctcgctctg gcacacatga tcaagtttcg aggtcatttc     2940
ctcatcgagg gcgatctcaa tcccgacaac agcgatgtgg acaagctgtt cattcagctc     3000
gttcagacct acaaccagct gttcgaggaa aaccccatca atgcctccgg agtcgatgca     3060
aaggccatct tgtctgctcg actctcgaag agcagacgac tggagaacct cattgcccaa     3120
cttcctggcg agaaaaagaa cggactgttt ggcaacctca ttgccctttc tcttggtctc     3180
acacccaact tcaagtccaa cttcgatctg gcggaggacg ccaagctcca gctgtccaag     3240
gacacctacg acgatgacct cgacaacctg cttgcacaga ttggcgatca gtacgccgac     3300
ctgtttctcg ctgccaagaa cctttcggat gctattctct tgtctgacat tctgcgagtc     3360
aacaccgaga tcacaaaggc tcccctttct gcctccatga tcaagcgata cgacgagcac     3420
catcaggatc tcacactgct caaggctctt gtccgacagc aactgcccga gaagtacaag     3480
gagatctttt tcgatcagtc gaagaacggc tacgctggat acatcgacgg cggagcctct     3540
caggaagagt tctacaagtt catcaagcca attctcgaga agatggacgg aaccgaggaa     3600
ctgcttgtca agctcaatcg agaggatctg cttcggaagc aacgaacctt cgacaacggc     3660
agcattcctc atcagatcca cctcggtgag ctgcacgcca ttcttcgacg tcaggaagac     3720
ttctacccct ttctcaagga caaccgagag aagatcgaga agattcttac ctttcgaatc     3780
ccctactatg ttggtcctct tgccagagga aactctcgat ttgcttggat gactcgaaag     3840
tccgaggaaa ccatcactcc ctggaacttc gaggaagtcg tggacaaggg tgcctctgca     3900
cagtccttca tcgagcgaat gaccaacttc gacaagaatc tgcccaacga gaaggttctt     3960
cccaagcatt cgctgctcta cgagtacttt acagtctaca acgaactcac caaagtcaag     4020
tacgttaccg agggaatgcg aaagcctgcc ttcttgtctg gcgaacagaa gaaagccatt     4080
gtcgatctcc tgttcaagac caaccgaaag gtcactgtta agcagctcaa ggaggactac     4140
ttcaagaaaa tcgagtgttt cgacagcgtc gagatttccg gagttgagga ccgattcaac     4200
gcctctttgg gcacctatca cgatctgctc aagattatca aggacaagga ttttctcgac     4260
aacgaggaaa acgaggacat tctggaggac atcgtgctca ctcttaccct gttcgaagat     4320
cgggagatga tcgaggaacg actcaagaca tacgctcacc tgttcgacga caaggtcatg     4380
aaacaactca agcgacgtag atacaccggc tggggaagac tttcgcgaaa gctcatcaac     4440
ggcatcagag acaagcagtc cggaaagacc attctggact ttctcaagtc cgatggcttt     4500
gccaaccgaa acttcatgca gctcattcac gacgattctc ttaccttcaa ggaggacatc     4560
cagaaggcac aagtgtccgg tcagggcgac agcttgcacg aacatattgc caacctggct     4620
ggttcgccag ccatcaagaa aggcattctc cagactgtca aggttgtcga cgagctggtg     4680
aaggtcatgg gacgtcacaa gcccgagaac attgtgatcg agatggccag agagaaccag     4740
acaactcaaa agggtcagaa aaactcgcga gagcggatga agcgaatcga ggaaggcatc     4800
aaggagctgg gatcccagat tctcaaggag catcccgtcg agaacactca actgcagaac     4860
gagaagctgt atctctacta tctgcagaat ggtcgagaca tgtacgtgga tcaggaactg     4920
gacatcaatc gtctcagcga ctacgatgtg gaccacattg tccctcaatc ctttctcaag     4980
gacgattcta tcgacaacaa ggtccttaca cgatccgaca agaacagagg caagtcggac     5040
aacgttccca gcgaagaggt ggtcaaaaag atgaagaact actggcgaca gctgctcaac     5100
gccaagctca ttacccagcg aaagttcgac aatcttacca aggccgagcg aggcggtctg     5160
tccgagctcg acaaggctgg cttcatcaag cgtcaactcg tcgagaccag acagatcaca     5220
aagcacgtcg cacagattct cgattctcgg atgaacacca agtacgacga gaacgacaag     5280
ctcatccgag aggtcaaggt gattactctc aagtccaaac tggtctccga tttccgaaag     5340
gactttcagt tctacaaggt gcgagagatc aacaattacc accatgccca cgatgcttac     5400
ctcaacgccg tcgttggcac tgcgctcatc aagaaatacc ccaagctcga aagcgagttc     5460
gtttacggcg attacaaggt ctacgacgtt cgaaagatga ttgccaagtc cgaacaggag     5520
attggcaagg ctactgccaa gtacttcttt tactccaaca tcatgaactt tttcaagacc     5580
gagatcacct tggccaacgg agagattcga aagagaccac ttatcgagac caacggcgaa     5640
actggagaga tcgtgtggga caagggtcga gactttgcaa ccgtgcgaaa ggttctgtcg     5700
atgcctcagg tcaacatcgt caagaaaacc gaggttcaga ctggcggatt ctccaaggag     5760
tcgattctgc ccaagcgaaa ctccgacaag ctcatcgctc gaaagaaaga ctgggatccc     5820
aagaaatacg gtggcttcga ttctcctacc gtcgcctatt ccgtgcttgt cgttgcgaag     5880
gtcgagaagg gcaagtccaa aaagctcaag tccgtcaagg agctgctcgg aattaccatc     5940
atggagcgat cgagcttcga gaagaatccc atcgacttct tggaagccaa gggttacaag     6000
gaggtcaaga aagacctcat tatcaagctg cccaagtact ctctgttcga actggagaac     6060
ggtcgaaagc gtatgctcgc ctccgctggc gagctgcaga agggaaacga gcttgccttg     6120
ccttcgaagt acgtcaactt tctctatctg gcttctcact acgagaagct caagggttct     6180
cccgaggaca acgaacagaa gcaactcttc gttgagcagc acaaacatta cctcgacgag     6240
attatcgagc agatttccga gttttcgaag cgagtcatcc tggctgatgc caacttggac     6300
aaggtgctct ctgcctacaa caagcatcgg gacaaaccca ttcgagaaca ggcggagaac     6360
atcattcacc tgtttactct taccaacctg ggtgctcctg cagctttcaa gtacttcgat     6420
accactatcg accgaaagcg gtacacatcc accaaggagg ttctcgatgc caccctgatt     6480
caccagtcca tcactggcct gtacgagacc cgaatcgacc tgtctcagct tggtggcgac     6540
tccagagccg atcccaagaa aaagcgaaag gtctaagcgg ccgcaagtgt ggatggggaa     6600
gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt caacacaggg     6660
atatagcgag ctacgtggtg gtgcgaggat atagcaacgg atatttatgt ttgacacttg     6720
agaatgtacg atacaagcac tgtccaagta caatactaaa catactgtac atactcatac     6780
tcgtacccgg gcaacggttt cacttgagtg cagtggctag tgctcttact cgtacagtgt     6840
gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta gttgcgtacg     6900
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat     6960
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg     7020
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct     7080
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     7140
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     7200
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     7260
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     7320
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     7380
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     7440
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     7500
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     7560
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     7620
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     7680
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     7740
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     7800
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     7860
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     7920
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     7980
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     8040
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     8100
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     8160
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     8220
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     8280
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     8340
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     8400
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     8460
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     8520
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     8580
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     8640
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     8700
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     8760
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     8820
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     8880
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     8940
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc     9000
gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac     9060
acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt     9120
cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc     9180
tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc     9240
gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact     9300
cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg     9360
gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc     9420
gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt     9480
tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt     9540
gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg     9600
acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc     9660
gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt cacacaaacc gatcttcgcc     9720
tcaaggaaac ctaattctac atccgagaga ctgccgagat ccagtctaca ctgattaatt     9780
ttcgggccaa taatttaaaa aaatcgtgtt atataatatt atatgtatta tatatataca     9840
tcatgatgat actgacagtc atgtcccatt gctaaataga cagactccat ctgccgcctc     9900
caactgatgt tctcaatatt taaggggtca tctcgcattg tttaataata aacagactcc     9960
atctaccgcc tccaaatgat gttctcaaaa tatattgtat gaacttattt ttattactta    10020
gtattattag acaacttact tgctttatga aaaacacttc ctatttagga aacaatttat    10080
aatggcagtt cgttcattta acaatttatg tagaataaat gttataaatg cgtatgggaa    10140
atcttaaata tggatagcat aaatgatatc tgcattgcct aattcgaaat caacagcaac    10200
gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa tatcaactat caaagaacag    10260
ctattcacac gttactattg agattattat tggacgagaa tcacacactc aactgtcttt    10320
ctctcttcta gaaatacagg tacaagtatg tactattctc attgttcata cttctagtca    10380
tttcatccca catattcctt ggatttctct ccaatgaatg acattctatc ttgcaaattc    10440
aacaattata ataagatata ccaaagtagc ggtatagtgg caatcaaaaa gcttctctgg    10500
tgtgcttctc gtatttattt ttattctaat gatccattaa aggtatatat ttatttcttg    10560
ttatataatc cttttgttta ttacatgggc tggatacata aaggtatttt gatttaattt    10620
tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt aatggtagga aattaccata    10680
cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg tatttccagg ttagacgttc    10740
cgcagaatct agaatgcggt atgcggtaca ttgttcttcg aacgtaaaag ttgcgctccc    10800
tgagatattg tacatttttg cttttacaag tacaagtaca tcgtacaact atgtactact    10860
gttgatgcat ccacaacagt ttgttttgtt tttttttgtt tttttttttt ctaatgattc    10920
attaccgcta tgtataccta cttgtacttg tagtaagccg ggttattggc gttcaattaa    10980
tcatagactt atgaatctgc acggtgtgcg ctgcgagtta cttttagctt atgcatgcta    11040
cttgggtgta atattgggat ctgttcggaa atcaacggat gctcaatcga taaaaaacaa    11100
aaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta    11160
acttgctatt tctagc                                                    11176

<210>  25
<211>  655
<212>  DNA
<213>  Artificial sequence

<220>
<223>  can1 upstream homology arm

<400>  25
gggaagcctt gctacgttag gagaagacgc acggcgatga tacgggtacc cctcatgaca       60
tcaatatccg ctgcccctct tgccagcaag gcgtcagcag gtgctttttt cgctattttc      120
accagaccac agcctttttc cttgtgtctc atcttggatt ccttcaaagg caactcaccg      180
cacctccgag tcgtgtgaac aatgtaataa taggctattg acttttttcc cacctgttta      240
gcgccaaacc caaagcgctt ttcgccccca ctgcagcccg atggaaggca catatggcaa      300
gggaaaagtc ttcaggtaat acatgcctgc tgcaactata tgtactctga ctcattccct      360
cagacgtggg tcatagacag ctgttttaaa ccgggcaaat caatctctgt cgcacaggta      420
tttctgccct tcaaaaccag gttgccacat cagattccat caaagttttt cagactaact      480
tcaatcttaa acggcatctc acaacaagcg aattggacgg aaaaaaagcg tctatcatta      540
ccggcaccta tccacactaa gacagtacta aaggacgacg ctccccacga aacgacgttt      600
cgaccttaac gaccctgccg tctccatcca tccgaccact cccgacgctc tctcc           655

<210>  26
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>   Can1 upstream forward

<400>  26
gggaagcttg ctacgttagg agaagacgc                                         29

<210>  27
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 upstream reverse

<400>  27
ggagagagcg tcgggagtgg tcggatggat ggagacg                                37

<210>  28
<211>  658
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 downstream homology arm

<400>  28
cgtctccatc catccgacca ctcccgacgc tctctcctgg agcaaaccac tcttaccaag       60
catatagcat atataataac gtattgaatt tattaactga ttgaattgag agtaaagcca      120
gtagcgttgt acggctgtag ctttttagaa aagtggcaga tgagcgatgg tggatatgaa      180
agtaccttta cggcatgtag cgacacaaga tcgcttccaa gaactcgaca ttcaagccca      240
gctcgtacaa gaaaatgaac tagccaatca tatgaactag cacattgaag tcaccgcatc      300
atctctgttg gaaacgacgc gcatgtactc gtgcgtagta aatccgtatc tgtacactcg      360
aaagattaca gtatgtagta gtagcatgac taacgatgta acgtccaaat aacgctctgt      420
gcctactcct gtagatgcat tagaccacct gctaacgtct acacgttatg tccgttagct      480
ccaagattgc acttttccct caaagactct gctgggttac gtcatggtct ctttcgggtc      540
tctggtccgt tctctgcccg cccatatccg cccaggctgc tacgatacag gataagctca      600
taagcttaga ttatttttcc ggaatgacat cacgatgcag tggtggaagg atgtatgg        658

<210>  29
<211>  37
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 downstream homology arm forward primer

<400>  29
cgtctccatc catccgacca ctcccgacgc tctctcc                                37

<210>  30
<211>  22
<212>  DNA
<213>  Yarrowia lipolytica

<400>  30
ccatacatcc ttccaccact gc                                                22

<210>  31
<211>  1276
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 editing template clonign fragment

<400>  31
gggaagcctt gctacgttag gagaagacgc acggcgatga tacgggtacc cctcatgaca       60
tcaatatccg ctgcccctct tgccagcaag gcgtcagcag gtgctttttt cgctattttc      120
accagaccac agcctttttc cttgtgtctc atcttggatt ccttcaaagg caactcaccg      180
cacctccgag tcgtgtgaac aatgtaataa taggctattg acttttttcc cacctgttta      240
gcgccaaacc caaagcgctt ttcgccccca ctgcagcccg atggaaggca catatggcaa      300
gggaaaagtc ttcaggtaat acatgcctgc tgcaactata tgtactctga ctcattccct      360
cagacgtggg tcatagacag ctgttttaaa ccgggcaaat caatctctgt cgcacaggta      420
tttctgccct tcaaaaccag gttgccacat cagattccat caaagttttt cagactaact      480
tcaatcttaa acggcatctc acaacaagcg aattggacgg aaaaaaagcg tctatcatta      540
ccggcaccta tccacactaa gacagtacta aaggacgacg ctccccacga aacgacgttt      600
cgaccttaac gaccctgccg tctccatcca tccgaccact cccgacgctc tctcctggag      660
caaaccactc ttaccaagca tatagcatat ataataacgt attgaattta ttaactgatt      720
gaattgagag taaagccagt agcgttgtac ggctgtagct ttttagaaaa gtggcagatg      780
agcgatggtg gatatgaaag tacctttacg gcatgtagcg acacaagatc gcttccaaga      840
actcgacatt caagcccagc tcgtacaaga aaatgaacta gccaatcata tgaactagca      900
cattgaagtc accgcatcat ctctgttgga aacgacgcgc atgtactcgt gcgtagtaaa      960
tccgtatctg tacactcgaa agattacagt atgtagtagt agcatgacta acgatgtaac     1020
gtccaaataa cgctctgtgc ctactcctgt agatgcatta gaccacctgc taacgtctac     1080
acgttatgtc cgttagctcc aagattgcac ttttccctca aagactctgc tgggttacgt     1140
catggtctct ttcgggtctc tggtccgttc tctgcccgcc catatccgcc caggctgcta     1200
cgatacagga taagctcata agcttagatt atttttccgg aatgacatca cgatgcagtg     1260
gtggaaggat gtatgg                                                     1276

<210>  32
<211>  2686
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pUC18

<400>  32
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360
tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg      420
actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct      480
gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt      540
aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc      600
gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg      660
agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg      720
gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca      780
gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac      840
cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac      900
aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg      960
tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac     1020
ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat     1080
ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag     1140
cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac     1200
ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt     1260
gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt     1320
atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc     1380
aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga     1440
aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac     1500
gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc     1560
cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct     1620
gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca     1680
tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct     1740
ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca     1800
ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc     1860
atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg     1920
cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct     1980
tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa     2040
aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta     2100
tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc     2160
ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg     2220
agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa     2280
gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg     2340
agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc     2400
accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg     2460
gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat     2520
cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata     2580
ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc     2640
atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc                    2686

<210>  33
<211>  3901
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF80

<400>  33
agcttgctac gttaggagaa gacgcacggc gatgatacgg gtacccctca tgacatcaat       60
atccgctgcc cctcttgcca gcaaggcgtc agcaggtgct tttttcgcta ttttcaccag      120
accacagcct ttttccttgt gtctcatctt ggattccttc aaaggcaact caccgcacct      180
ccgagtcgtg tgaacaatgt aataataggc tattgacttt tttcccacct gtttagcgcc      240
aaacccaaag cgcttttcgc ccccactgca gcccgatgga aggcacatat ggcaagggaa      300
aagtcttcag gtaatacatg cctgctgcaa ctatatgtac tctgactcat tccctcagac      360
gtgggtcata gacagctgtt ttaaaccggg caaatcaatc tctgtcgcac aggtatttct      420
gcccttcaaa accaggttgc cacatcagat tccatcaaag tttttcagac taacttcaat      480
cttaaacggc atctcacaac aagcgaattg gacggaaaaa aagcgtctat cattaccggc      540
acctatccac actaagacag tactaaagga cgacgctccc cacgaaacga cgtttcgacc      600
ttaacgaccc tgccgtctcc atccatccga ccactcccga cgctctctcc tggagcaaac      660
cactcttacc aagcatatag catatataat aacgtattga atttattaac tgattgaatt      720
gagagtaaag ccagtagcgt tgtacggctg tagcttttta gaaaagtggc agatgagcga      780
tggtggatat gaaagtacct ttacggcatg tagcgacaca agatcgcttc caagaactcg      840
acattcaagc ccagctcgta caagaaaatg aactagccaa tcatatgaac tagcacattg      900
aagtcaccgc atcatctctg ttggaaacga cgcgcatgta ctcgtgcgta gtaaatccgt      960
atctgtacac tcgaaagatt acagtatgta gtagtagcat gactaacgat gtaacgtcca     1020
aataacgctc tgtgcctact cctgtagatg cattagacca cctgctaacg tctacacgtt     1080
atgtccgtta gctccaagat tgcacttttc cctcaaagac tctgctgggt tacgtcatgg     1140
tctctttcgg gtctctggtc cgttctctgc ccgcccatat ccgcccaggc tgctacgata     1200
caggataagc tcataagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg     1260
agctcgaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca     1320
attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg     1380
agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg     1440
tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc     1500
tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta     1560
tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag     1620
aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg     1680
tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg     1740
tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg     1800
cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga     1860
agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc     1920
tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt     1980
aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact     2040
ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg     2100
cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt     2160
accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt     2220
ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct     2280
ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg     2340
gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt     2400
aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt     2460
gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc     2520
gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg     2580
cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc     2640
gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg     2700
gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca     2760
ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga     2820
tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct     2880
ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg     2940
cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca     3000
accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata     3060
cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct     3120
tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact     3180
cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa     3240
acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc     3300
atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga     3360
tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga     3420
aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg     3480
cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac     3540
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc     3600
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca     3660
gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg     3720
agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga     3780
tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga     3840
ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc     3900
a                                                                     3901

<210>  34
<211>  1210
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 polynucleotide modification (editing) template

<400>  34
gctacgttag gagaagacgc acggcgatga tacgggtacc cctcatgaca tcaatatccg       60
ctgcccctct tgccagcaag gcgtcagcag gtgctttttt cgctattttc accagaccac      120
agcctttttc cttgtgtctc atcttggatt ccttcaaagg caactcaccg cacctccgag      180
tcgtgtgaac aatgtaataa taggctattg acttttttcc cacctgttta gcgccaaacc      240
caaagcgctt ttcgccccca ctgcagcccg atggaaggca catatggcaa gggaaaagtc      300
ttcaggtaat acatgcctgc tgcaactata tgtactctga ctcattccct cagacgtggg      360
tcatagacag ctgttttaaa ccgggcaaat caatctctgt cgcacaggta tttctgccct      420
tcaaaaccag gttgccacat cagattccat caaagttttt cagactaact tcaatcttaa      480
acggcatctc acaacaagcg aattggacgg aaaaaaagcg tctatcatta ccggcaccta      540
tccacactaa gacagtacta aaggacgacg ctccccacga aacgacgttt cgaccttaac      600
gaccctgccg tctccatcca tccgaccact cccgacgctc tctcctggag caaaccactc      660
ttaccaagca tatagcatat ataataacgt attgaattta ttaactgatt gaattgagag      720
taaagccagt agcgttgtac ggctgtagct ttttagaaaa gtggcagatg agcgatggtg      780
gatatgaaag tacctttacg gcatgtagcg acacaagatc gcttccaaga actcgacatt      840
caagcccagc tcgtacaaga aaatgaacta gccaatcata tgaactagca cattgaagtc      900
accgcatcat ctctgttgga aacgacgcgc atgtactcgt gcgtagtaaa tccgtatctg      960
tacactcgaa agattacagt atgtagtagt agcatgacta acgatgtaac gtccaaataa     1020
cgctctgtgc ctactcctgt agatgcatta gaccacctgc taacgtctac acgttatgtc     1080
cgttagctcc aagattgcac ttttccctca aagactctgc tgggttacgt catggtctct     1140
ttcgggtctc tggtccgttc tctgcccgcc catatccgcc caggctgcta cgatacagga     1200
taagctcata                                                            1210

<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  C3S forward

<400>  35
agcttgctac gttaggagaa                                                   20

<210>  36
<211>  20
<212>  DNA
<213>  Artificial seqeunce

<220>
<223>  C3S reverse

<400>  36
tatgagctta tcctgtatcg                                                   20

<210>  37
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PT forward

<400>  37
agcttgctac gttaggagaa                                                   20

<210>  38
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PT reverse

<400>  38
tatgagctta tcctgtatcg                                                   20

<210>  39
<211>  2125
<212>  DNA
<213>  Artificial sequence

<220>
<223>  CAN1 locus

<400>  39
ggaaggcaca tatggcaagg gaaaagtctt caggtaatac atgcctgctg caactatatg       60
tactctgact cattccctca gacgtgggtc atagacagct gttttaaacc gggcaaatca      120
atctctgtcg cacaggtatt tctgcccttc aaaaccaggt tgccacatca gattccatca      180
aagtttttca gactaacttc aatcttaaac ggcatctcac aacaagcgaa ttggacggaa      240
aaaaagcgtc tatcattacc ggcacctatc cacactaaga cagtactaaa ggacgacgct      300
ccccacgaaa cgacgtttcg accttaacga ccctgccgtc tccatccatc cgaccacaat      360
ggaaaagaca ttttcaaacg attacccacc ctccgggact gaggcccaca tccacatcaa      420
ccacacggcc cactcggatg actcagagga ggtgccctcg cacaaggaaa attacaacac      480
cagtggccac gacctggagg agtccgaccc ggataaccat gtcggtgaga ccctcgaggt      540
caagcgaggt ctcaagatgc gacacatctc catgatctcg cttggaggaa ccattggtac      600
cggtctcttc attggtaccg gaggagctct ccagcaggcc ggtccctgtg gcgccctcgt      660
cgcctacgtg ttcatggcca ccattgtcta ctctgttgcc gagtctcttg gagaactggc      720
tacgtacatt cccatcaccg gctcctttgc cgtctttact acccgatatc tgtcacagtc      780
gtttggtgcc tccatgggct ggctatactg gttctcgtgg gcgatcacct tcgccatcga      840
gctcaacacc attggtcccg tgattgagta ctggactgac gccgttccta ctgctgcctg      900
gattgccatc ttcttcgtca tcctcactac catcaacttc ttccccgtgg gcttctatgg      960
cgaagtcgag ttctgggtgg cctccgtgaa ggtcattgcc atcattggat ggctcatcta     1020
cgcgctctgc atgacgtgtg gagcaggtgt aacaggtcct gtgggattca gatactggaa     1080
ccaccccgga cccatgggag acggaatctg gaccgacggc gtgcccattg tgcgaaacgc     1140
gcccggtcga cgattcatgg gatggctcaa ttcgctcgtt aacgccgcct tcacctacca     1200
gggctgtgag ctggtcggag tcactgccgg tgaggcccag aaccccagaa agtccgtccc     1260
tcgagccatc aaccgagtct ttgctcgaat ttgcatcttc tacattggct ctatcttctt     1320
catgggcatg ctcgtgccct ttaacgaccc caagctgacc gatgactcct ccgtcatcgc     1380
ctcctctcct tttgttattg ccattatcaa ctctggcacc aaggtgctcc ctcacatttt     1440
caacgccgtc attctcatca ccctgatttc ggcaggaaac tccaacgtct acattggctc     1500
gcgagtggtc tacgccctgg ctgactccgg aaccgcacca aagttcttca agcgaaccac     1560
caagaaggga gtgccgtacg tggcagtctg cttcacctcg gcgtttggtc tgctggcctt     1620
catgtctgtg tccgagtcgt cgtccactgt cttcgactgg ttcatcaaca tctccgctgt     1680
ggccggcctc atctgttggg ccttcatctc tgcctcccac atccgattca tgcaagtgct     1740
taagcacaga gggatctcca gagatacgct gcccttcaag gcacgatggc agccattcta     1800
ctcatggtac gcgctcgtct ccatcatctt catcactctc atccagggct tcacgtcctt     1860
ctggcacttt accgccgcca agttcatgac tgcatacatc tccgtcattg tctgggtcgg     1920
tttgtacatt atcttccagt gtctgttccg atgcaagttc cttatcccta ttgaggatgt     1980
ggacattgac accggccgac gagagattga cgacgatgtg tgggaggaga agatccccac     2040
aaagtggtac gagaagtttt ggaatattat tgcataagaa gatcggggat tcccgacgct     2100
ctctcctgga gcaaaccact cttac                                           2125

<210>  40
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  unmodified forward

<400>  40
agcttgctac gttaggagaa                                                   20

<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  unmodified reverse

<400>  41
tatgagctta tcctgtatcg                                                   20

<210>  42
<211>  20
<212>  DNA
<213>  Yarrowia lipolytica

<400>  42
ggaaggcaca tatggcaagg                                                   20

<210>  43
<211>  21
<212>  DNA
<213>  Yarrowia lipolytica

<400>  43
gtaagagtgg tttgctccag g                                                 21

<210>  44
<211>  2125
<212>  DNA
<213>  Yarrowia lipolytica

<400>  44
ggaaggcaca tatggcaagg gaaaagtctt caggtaatac atgcctgctg caactatatg       60
tactctgact cattccctca gacgtgggtc atagacagct gttttaaacc gggcaaatca      120
atctctgtcg cacaggtatt tctgcccttc aaaaccaggt tgccacatca gattccatca      180
aagtttttca gactaacttc aatcttaaac ggcatctcac aacaagcgaa ttggacggaa      240
aaaaagcgtc tatcattacc ggcacctatc cacactaaga cagtactaaa ggacgacgct      300
ccccacgaaa cgacgtttcg accttaacga ccctgccgtc tccatccatc cgaccacaat      360
ggaaaagaca ttttcaaacg attacccacc ctccgggact gaggcccaca tccacatcaa      420
ccacacggcc cactcggatg actcagagga ggtgccctcg cacaaggaaa attacaacac      480
cagtggccac gacctggagg agtccgaccc ggataaccat gtcggtgaga ccctcgaggt      540
caagcgaggt ctcaagatgc gacacatctc catgatctcg cttggaggaa ccattggtac      600
cggtctcttc attggtaccg gaggagctct ccagcaggcc ggtccctgtg gcgccctcgt      660
cgcctacgtg ttcatggcca ccattgtcta ctctgttgcc gagtctcttg gagaactggc      720
tacgtacatt cccatcaccg gctcctttgc cgtctttact acccgatatc tgtcacagtc      780
gtttggtgcc tccatgggct ggctatactg gttctcgtgg gcgatcacct tcgccatcga      840
gctcaacacc attggtcccg tgattgagta ctggactgac gccgttccta ctgctgcctg      900
gattgccatc ttcttcgtca tcctcactac catcaacttc ttccccgtgg gcttctatgg      960
cgaagtcgag ttctgggtgg cctccgtgaa ggtcattgcc atcattggat ggctcatcta     1020
cgcgctctgc atgacgtgtg gagcaggtgt aacaggtcct gtgggattca gatactggaa     1080
ccaccccgga cccatgggag acggaatctg gaccgacggc gtgcccattg tgcgaaacgc     1140
gcccggtcga cgattcatgg gatggctcaa ttcgctcgtt aacgccgcct tcacctacca     1200
gggctgtgag ctggtcggag tcactgccgg tgaggcccag aaccccagaa agtccgtccc     1260
tcgagccatc aaccgagtct ttgctcgaat ttgcatcttc tacattggct ctatcttctt     1320
catgggcatg ctcgtgccct ttaacgaccc caagctgacc gatgactcct ccgtcatcgc     1380
ctcctctcct tttgttattg ccattatcaa ctctggcacc aaggtgctcc ctcacatttt     1440
caacgccgtc attctcatca ccctgatttc ggcaggaaac tccaacgtct acattggctc     1500
gcgagtggtc tacgccctgg ctgactccgg aaccgcacca aagttcttca agcgaaccac     1560
caagaaggga gtgccgtacg tggcagtctg cttcacctcg gcgtttggtc tgctggcctt     1620
catgtctgtg tccgagtcgt cgtccactgt cttcgactgg ttcatcaaca tctccgctgt     1680
ggccggcctc atctgttggg ccttcatctc tgcctcccac atccgattca tgcaagtgct     1740
taagcacaga gggatctcca gagatacgct gcccttcaag gcacgatggc agccattcta     1800
ctcatggtac gcgctcgtct ccatcatctt catcactctc atccagggct tcacgtcctt     1860
ctggcacttt accgccgcca agttcatgac tgcatacatc tccgtcattg tctgggtcgg     1920
tttgtacatt atcttccagt gtctgttccg atgcaagttc cttatcccta ttgaggatgt     1980
ggacattgac accggccgac gagagattga cgacgatgtg tgggaggaga agatccccac     2040
aaagtggtac gagaagtttt ggaatattat tgcataagaa gatcggggat tcccgacgct     2100
ctctcctgga gcaaaccact cttac                                           2125

<210>  45
<211>  392
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 locus deletion

<400>  45
ggaaggcaca tatggcaagg gaaaagtctt caggtaatac atgcctgctg caactatatg       60
tactctgact cattccctca gacgtgggtc atagacagct gttttaaacc gggcaaatca      120
atctctgtcg cacaggtatt tctgcccttc aaaaccaggt tgccacatca gattccatca      180
aagtttttca gactaacttc aatcttaaac ggcatctcac aacaagcgaa ttggacggaa      240
aaaaagcgtc tatcattacc ggcacctatc cacactaaga cagtactaaa ggacgacgct      300
ccccacgaaa cgacgtttcg accttaacga ccctgccgtc tccatccatc cgaccactcc      360
cgacgctctc tcctggagca aaccactctt ac                                    392

<210>  46
<211>  62
<212>  DNA
<213>  Yarrowia lipolytica

<220>
<221>  misc_feature
<222>  (1)..(62)
<223>  Copy number analysis fragment

<400>  46
agcgccaaac ccaaagcgct tttcgccccc actgcagccc gatggaaggc acatatggca       60
ag                                                                      62

<210>  47
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 copy number F

<400>  47
agcgccaaac ccaaagc                                                      17

<210>  48
<211>  20
<212>  DNA
<213>  Artificial Seqeunce

<220>
<223>  Can1 copy number R

<400>  48
cttgccatat gtgccttcca                                                   20

<210>  49
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Can1 copy number probe

<400>  49
cttttcgccc ccactgcagc c                                                 21

<210>  50
<211>  69
<212>  DNA
<213>  Yarrowia lipolytica

<400>  50
tgaccgtcct tggagatacc agcctcgaac tcaccaacac caccagcaat gatgaggatg       60
gcacagtcg                                                               69

<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TEF1 forward

<400>  51
cgactgtgcc atcctcatca                                                   20

<210>  52
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TEF1 reverse

<400>  52
tgaccgtcct tggagatacc a                                                 21

<210>  53
<211>  21
<212>  DNA
<213>  Yarrowia lipolytica

<400>  53
tgctggtggt gttggtgagt t                                                 21

<210>  54
<211>  12167
<212>  DNA
<213>  Artificial sequence

<220>
<223>   pRF434

<400>  54
cgataaaaaa caaaaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac       60
tagccttatt ttaacttgct atttctagct ctaaaacgca ggtgtaaaaa taaaaaggcc      120
tgcgattacc agcaggcctg ttattaacct aagccttagg acgcttcacg ccatacttgg      180
aacgagcctg cttacggtct ttaacgccgg agcagtcaag cgcaccacgt acggtgtggt      240
aacgaacacc cgggaggtct ttaacacgac cgccacggat caggatcacg gagtgctcct      300
gcaggttgtg accttcacca ccgatgtagg aagtcacttc gaaaccgtta gtcagacgaa      360
cacggcatac tttacgcagc gcggagttcg gttttttagg agtggtagta tatacacgag      420
tacatacgcc acgtttttgc gggcatgctt ccagcgcagg cacgttgctt ttcgcaactt      480
tgcgagcacg tggtttgcgt accagctggt taactgttgc cattaaatag ctcctggttt      540
tagcttttgc ttcgtaaaca cgtaataaaa cgtcctcaca caatatgagg acgccgaatt      600
tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca cctgcgtccc      660
attcgccatg ccgaagcatg ttgcccagcc ggcgccagcg aggaggctgg gaccatgccg      720
gccattattt tgcgttaagt ttctaatcat cacgaaatta tctatcaaaa ataactaggt      780
cccaccgaga ttcgaactcg ggaccttaag atttgcaatc tcacgcgcta ccgctgtgcc      840
ataggaccga agttaaaatt tggccaaaga aggacctggg caccctggac tgtgggttag      900
ggtaatattc cttatggaga caatgggcta gggtaaatta cctaaaatgg gtcgataaag      960
aggggtgttc ccagttggga agtgtaattg aagacggggt caaaaaagaa aatcaaaaaa     1020
aatttaatta agactatgat aacttcgtat aatgtatgct atacgaacgg tagcacactg     1080
tacgagtaag agcactagcc actgcactca agtgaaaccg ttgcccgggt acgagtatga     1140
gtatgtacag tatgtttagt attgtacttg gacagtgctt gtatcgtaca ttctcaagtg     1200
tcaaacataa atatccgttg ctatatcctc gcaccaccac gtagctcgct atatccctgt     1260
gttgaatcca tccatcttgg attgccaatt gtgcacacag aaccgggcac tcacttcccc     1320
atccacactt gcggccgcta ttcctttgcc ctcggacgag tgctggggcg tcggtttcca     1380
ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt     1440
tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc     1500
caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg     1560
cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag     1620
tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc     1680
tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca     1740
ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg     1800
cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg     1860
tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca     1920
cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg     1980
ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggctgcag aacagcgggc     2040
agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag     2100
gtcaggctct cgctgaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat     2160
gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc     2220
aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag     2280
agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc     2340
gcggtgagtt caggcttttt ggccatggtt gatgtgtgtt taattcaaga atgaatatag     2400
agaagagaag aagaaaaaag attcaattga gccggcgatg cagaccctta tataaatgtt     2460
gccttggaca gacggagcaa gcccgcccaa acctacgttc ggtataatat gttaagcttt     2520
ttaacacaaa ggtttggctt ggggtaacct gatgtggtgc aaaagaccgg gcgttggcga     2580
gccattgcgc gggcgaatgg ggccgtgact cgtctcaaat tcgagggcgt gcctcaattc     2640
gtgcccccgt ggctttttcc cgccgtttcc gccccgtttg caccactgca gccgcttctt     2700
tggttcggac accttgctgc gagctaggtg ccttgtgcta cttaaaaagt ggcctcccaa     2760
caccaacatg acatgagtgc gtgggccaag acacgttggc ggggtcgcag tcggctcaat     2820
ggcccggaaa aaacgctgct ggagctggtt cggacgcagt ccgccgcggc gtatggatat     2880
ccgcaaggtt ccatagcgcc attgccctcc gtcggcgtct atcccgcaac ctaccgttcg     2940
tataatgtat gctatacgaa gttatgagcg ggcttaaggt ttaaaccatc atctaagggc     3000
ctcaaaacta cctcggaact gctgcgctga tctggacacc acagaggttc cgagcacttt     3060
aggttgcacc aaatgtccca ccaggtgcag gcagaaaacg ctggaacagc gtgtacagtt     3120
tgtcttaaca aaaagtgagg gcgctgaggt cgagcagggt ggtgtgactt gttatagcct     3180
ttagagctgc gaaagcgcgt atggatttgg ctcatcaggc cagattgagg gtctgtggac     3240
acatgtcatg ttagtgtact tcaatcgccc cctggatata gccccgacaa taggccgtgg     3300
cctcattttt ttgccttccg cacatttcca ttgctcggta cccacacctt gcttctcctg     3360
cacttgccaa ccttaatact ggtttacatt gaccaacatc ttacaagcgg ggggcttgtc     3420
tagggtatat ataaacagtg gctctcccaa tcggttgcca gtctcttttt tcctttcttt     3480
ccccacagat tcgaaatcta aactacacat cacaccatgg acaagaaata ctccatcggc     3540
ctggacattg gaaccaactc tgtcggctgg gctgtcatca ccgacgagta caaggtgccc     3600
tccaagaaat tcaaggtcct cggaaacacc gatcgacact ccatcaagaa aaacctcatt     3660
ggtgccctgt tgttcgattc tggcgagact gccgaagcta ccagactcaa gcgaactgct     3720
cggcgacgtt acacccgacg gaagaaccga atctgctacc tgcaggagat cttttccaac     3780
gagatggcca aggtggacga ttcgttcttt catcgactgg aggaatcctt cctcgtcgag     3840
gaagacaaga aacacgagcg tcatcccatc tttggcaaca ttgtggacga ggttgcttac     3900
cacgagaagt atcctaccat ctaccatctc cgaaagaaac tcgtcgattc caccgacaag     3960
gcggatctca gacttatcta cctcgctctg gcacacatga tcaagtttcg aggtcatttc     4020
ctcatcgagg gcgatctcaa tcccgacaac agcgatgtgg acaagctgtt cattcagctc     4080
gttcagacct acaaccagct gttcgaggaa aaccccatca atgcctccgg agtcgatgca     4140
aaggccatct tgtctgctcg actctcgaag agcagacgac tggagaacct cattgcccaa     4200
cttcctggcg agaaaaagaa cggactgttt ggcaacctca ttgccctttc tcttggtctc     4260
acacccaact tcaagtccaa cttcgatctg gcggaggacg ccaagctcca gctgtccaag     4320
gacacctacg acgatgacct cgacaacctg cttgcacaga ttggcgatca gtacgccgac     4380
ctgtttctcg ctgccaagaa cctttcggat gctattctct tgtctgacat tctgcgagtc     4440
aacaccgaga tcacaaaggc tcccctttct gcctccatga tcaagcgata cgacgagcac     4500
catcaggatc tcacactgct caaggctctt gtccgacagc aactgcccga gaagtacaag     4560
gagatctttt tcgatcagtc gaagaacggc tacgctggat acatcgacgg cggagcctct     4620
caggaagagt tctacaagtt catcaagcca attctcgaga agatggacgg aaccgaggaa     4680
ctgcttgtca agctcaatcg agaggatctg cttcggaagc aacgaacctt cgacaacggc     4740
agcattcctc atcagatcca cctcggtgag ctgcacgcca ttcttcgacg tcaggaagac     4800
ttctacccct ttctcaagga caaccgagag aagatcgaga agattcttac ctttcgaatc     4860
ccctactatg ttggtcctct tgccagagga aactctcgat ttgcttggat gactcgaaag     4920
tccgaggaaa ccatcactcc ctggaacttc gaggaagtcg tggacaaggg tgcctctgca     4980
cagtccttca tcgagcgaat gaccaacttc gacaagaatc tgcccaacga gaaggttctt     5040
cccaagcatt cgctgctcta cgagtacttt acagtctaca acgaactcac caaagtcaag     5100
tacgttaccg agggaatgcg aaagcctgcc ttcttgtctg gcgaacagaa gaaagccatt     5160
gtcgatctcc tgttcaagac caaccgaaag gtcactgtta agcagctcaa ggaggactac     5220
ttcaagaaaa tcgagtgttt cgacagcgtc gagatttccg gagttgagga ccgattcaac     5280
gcctctttgg gcacctatca cgatctgctc aagattatca aggacaagga ttttctcgac     5340
aacgaggaaa acgaggacat tctggaggac atcgtgctca ctcttaccct gttcgaagat     5400
cgggagatga tcgaggaacg actcaagaca tacgctcacc tgttcgacga caaggtcatg     5460
aaacaactca agcgacgtag atacaccggc tggggaagac tttcgcgaaa gctcatcaac     5520
ggcatcagag acaagcagtc cggaaagacc attctggact ttctcaagtc cgatggcttt     5580
gccaaccgaa acttcatgca gctcattcac gacgattctc ttaccttcaa ggaggacatc     5640
cagaaggcac aagtgtccgg tcagggcgac agcttgcacg aacatattgc caacctggct     5700
ggttcgccag ccatcaagaa aggcattctc cagactgtca aggttgtcga cgagctggtg     5760
aaggtcatgg gacgtcacaa gcccgagaac attgtgatcg agatggccag agagaaccag     5820
acaactcaaa agggtcagaa aaactcgcga gagcggatga agcgaatcga ggaaggcatc     5880
aaggagctgg gatcccagat tctcaaggag catcccgtcg agaacactca actgcagaac     5940
gagaagctgt atctctacta tctgcagaat ggtcgagaca tgtacgtgga tcaggaactg     6000
gacatcaatc gtctcagcga ctacgatgtg gaccacattg tccctcaatc ctttctcaag     6060
gacgattcta tcgacaacaa ggtccttaca cgatccgaca agaacagagg caagtcggac     6120
aacgttccca gcgaagaggt ggtcaaaaag atgaagaact actggcgaca gctgctcaac     6180
gccaagctca ttacccagcg aaagttcgac aatcttacca aggccgagcg aggcggtctg     6240
tccgagctcg acaaggctgg cttcatcaag cgtcaactcg tcgagaccag acagatcaca     6300
aagcacgtcg cacagattct cgattctcgg atgaacacca agtacgacga gaacgacaag     6360
ctcatccgag aggtcaaggt gattactctc aagtccaaac tggtctccga tttccgaaag     6420
gactttcagt tctacaaggt gcgagagatc aacaattacc accatgccca cgatgcttac     6480
ctcaacgccg tcgttggcac tgcgctcatc aagaaatacc ccaagctcga aagcgagttc     6540
gtttacggcg attacaaggt ctacgacgtt cgaaagatga ttgccaagtc cgaacaggag     6600
attggcaagg ctactgccaa gtacttcttt tactccaaca tcatgaactt tttcaagacc     6660
gagatcacct tggccaacgg agagattcga aagagaccac ttatcgagac caacggcgaa     6720
actggagaga tcgtgtggga caagggtcga gactttgcaa ccgtgcgaaa ggttctgtcg     6780
atgcctcagg tcaacatcgt caagaaaacc gaggttcaga ctggcggatt ctccaaggag     6840
tcgattctgc ccaagcgaaa ctccgacaag ctcatcgctc gaaagaaaga ctgggatccc     6900
aagaaatacg gtggcttcga ttctcctacc gtcgcctatt ccgtgcttgt cgttgcgaag     6960
gtcgagaagg gcaagtccaa aaagctcaag tccgtcaagg agctgctcgg aattaccatc     7020
atggagcgat cgagcttcga gaagaatccc atcgacttct tggaagccaa gggttacaag     7080
gaggtcaaga aagacctcat tatcaagctg cccaagtact ctctgttcga actggagaac     7140
ggtcgaaagc gtatgctcgc ctccgctggc gagctgcaga agggaaacga gcttgccttg     7200
ccttcgaagt acgtcaactt tctctatctg gcttctcact acgagaagct caagggttct     7260
cccgaggaca acgaacagaa gcaactcttc gttgagcagc acaaacatta cctcgacgag     7320
attatcgagc agatttccga gttttcgaag cgagtcatcc tggctgatgc caacttggac     7380
aaggtgctct ctgcctacaa caagcatcgg gacaaaccca ttcgagaaca ggcggagaac     7440
atcattcacc tgtttactct taccaacctg ggtgctcctg cagctttcaa gtacttcgat     7500
accactatcg accgaaagcg gtacacatcc accaaggagg ttctcgatgc caccctgatt     7560
caccagtcca tcactggcct gtacgagacc cgaatcgacc tgtctcagct tggtggcgac     7620
tccagagccg atcccaagaa aaagcgaaag gtctaagcgg ccgcaagtgt ggatggggaa     7680
gtgagtgccc ggttctgtgt gcacaattgg caatccaaga tggatggatt caacacaggg     7740
atatagcgag ctacgtggtg gtgcgaggat atagcaacgg atatttatgt ttgacacttg     7800
agaatgtacg atacaagcac tgtccaagta caatactaaa catactgtac atactcatac     7860
tcgtacccgg gcaacggttt cacttgagtg cagtggctag tgctcttact cgtacagtgt     7920
gcaatactgc gtatcatagt ctttgatgta tatcgtattc attcatgtta gttgcgtacg     7980
agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat     8040
tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg     8100
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct     8160
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     8220
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     8280
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     8340
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     8400
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     8460
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     8520
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     8580
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     8640
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     8700
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     8760
tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     8820
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     8880
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     8940
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     9000
aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     9060
atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     9120
gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     9180
acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     9240
ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     9300
tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     9360
ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     9420
ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     9480
atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     9540
taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     9600
catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     9660
atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     9720
acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     9780
aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     9840
ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     9900
cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     9960
atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat    10020
ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc    10080
gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac    10140
acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt    10200
cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc    10260
tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc    10320
gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact    10380
cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg    10440
gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc    10500
gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt    10560
tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt    10620
gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg    10680
acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc    10740
gaggtcgatg gtgtcgataa gcttgatatc gaattcatgt cacacaaacc gatcttcgcc    10800
tcaaggaaac ctaattctac atccgagaga ctgccgagat ccagtctaca ctgattaatt    10860
ttcgggccaa taatttaaaa aaatcgtgtt atataatatt atatgtatta tatatataca    10920
tcatgatgat actgacagtc atgtcccatt gctaaataga cagactccat ctgccgcctc    10980
caactgatgt tctcaatatt taaggggtca tctcgcattg tttaataata aacagactcc    11040
atctaccgcc tccaaatgat gttctcaaaa tatattgtat gaacttattt ttattactta    11100
gtattattag acaacttact tgctttatga aaaacacttc ctatttagga aacaatttat    11160
aatggcagtt cgttcattta acaatttatg tagaataaat gttataaatg cgtatgggaa    11220
atcttaaata tggatagcat aaatgatatc tgcattgcct aattcgaaat caacagcaac    11280
gaaaaaaatc ccttgtacaa cataaatagt catcgagaaa tatcaactat caaagaacag    11340
ctattcacac gttactattg agattattat tggacgagaa tcacacactc aactgtcttt    11400
ctctcttcta gaaatacagg tacaagtatg tactattctc attgttcata cttctagtca    11460
tttcatccca catattcctt ggatttctct ccaatgaatg acattctatc ttgcaaattc    11520
aacaattata ataagatata ccaaagtagc ggtatagtgg caatcaaaaa gcttctctgg    11580
tgtgcttctc gtatttattt ttattctaat gatccattaa aggtatatat ttatttcttg    11640
ttatataatc cttttgttta ttacatgggc tggatacata aaggtatttt gatttaattt    11700
tttgcttaaa ttcaatcccc cctcgttcag tgtcaactgt aatggtagga aattaccata    11760
cttttgaaga agcaaaaaaa atgaaagaaa aaaaaaatcg tatttccagg ttagacgttc    11820
cgcagaatct agaatgcggt atgcggtaca ttgttcttcg aacgtaaaag ttgcgctccc    11880
tgagatattg tacatttttg cttttacaag tacaagtaca tcgtacaact atgtactact    11940
gttgatgcat ccacaacagt ttgttttgtt tttttttgtt tttttttttt ctaatgattc    12000
attaccgcta tgtataccta cttgtacttg tagtaagccg ggttattggc gttcaattaa    12060
tcatagactt atgaatctgc acggtgtgcg ctgcgagtta cttttagctt atgcatgcta    12120
cttgggtgta atattgggat ctgttcggaa atcaacggat gctcaat                  12167

<210>  55
<211>  1963
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Hygromycin resistance cassette

<400>  55
gtttaaacct taagcccgct cataacttcg tatagcatac attatacgaa cggtaggttg       60
cgggatagac gccgacggag ggcaatggcg ctatggaacc ttgcggatat ccatacgccg      120
cggcggactg cgtccgaacc agctccagca gcgttttttc cgggccattg agccgactgc      180
gaccccgcca acgtgtcttg gcccacgcac tcatgtcatg ttggtgttgg gaggccactt      240
tttaagtagc acaaggcacc tagctcgcag caaggtgtcc gaaccaaaga agcggctgca      300
gtggtgcaaa cggggcggaa acggcgggaa aaagccacgg gggcacgaat tgaggcacgc      360
cctcgaattt gagacgagtc acggccccat tcgcccgcgc aatggctcgc caacgcccgg      420
tcttttgcac cacatcaggt taccccaagc caaacctttg tgttaaaaag cttaacatat      480
tataccgaac gtaggtttgg gcgggcttgc tccgtctgtc caaggcaaca tttatataag      540
ggtctgcatc gccggctcaa ttgaatcttt tttcttcttc tcttctctat attcattctt      600
gaattaaaca cacatcaacc atggccaaaa agcctgaact caccgcgacg tctgtcgaga      660
agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag      720
aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct      780
gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc      840
cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc      900
gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc      960
agccggtcgc ggaggctatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt     1020
tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg     1080
cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt     1140
ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc     1200
acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag     1260
cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct     1320
tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc     1380
atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc     1440
aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat     1500
gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa     1560
gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc     1620
ccagcactcg tccgagggca aaggaatagc ggccgcaagt gtggatgggg aagtgagtgc     1680
ccggttctgt gtgcacaatt ggcaatccaa gatggatgga ttcaacacag ggatatagcg     1740
agctacgtgg tggtgcgagg atatagcaac ggatatttat gtttgacact tgagaatgta     1800
cgatacaagc actgtccaag tacaatacta aacatactgt acatactcat actcgtaccc     1860
gggcaacggt ttcacttgag tgcagtggct agtgctctta ctcgtacagt gtgctaccgt     1920
tcgtatagca tacattatac gaagttatca tagtcttaat taa                       1963

<210>  56
<211>  1714
<212>  DNA
<213>  Yarrowia lipolytica

<400>  56
catgacatgt gtccacagac cctcaatctg gcctgatgag ccaaatccat acgcgctttc       60
gcagctctaa aggctataac aagtcacacc accctgctcg acctcagcgc cctcactttt      120
tgttaagaca aactgtacac gctgttccag cgttttctgc ctgcacctgg tgggacattt      180
ggtgcaacct aaagtgctcg gaacctctgt ggtgtccaga tcagcgcagc agttccgagg      240
tagttttgag gcccttagat gatggtttaa acgtcgacga gtatctgtct gactcgtcat      300
tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg acgatacatt      360
cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc gacaacaata      420
tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc aaaggcgacg      480
cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt ccagtctatc      540
tataagttca actaactcgt aactattacc ataacatata cttcactgcc ccagataagg      600
ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc accaaaatgc      660
cctcctacga agctcgagct aacgtccaca agtccgcctt tgccgctcga gtgctcaagc      720
tcgtggcagc caagaaaacc aacctgtgtg cttctctgga tgttaccacc accaaggagc      780
tcattgagct tgccgataag gtcggacctt atgtgtgcat gatcaaaacc catatcgaca      840
tcattgacga cttcacctac gccggcactg tgctccccct caaggaactt gctcttaagc      900
acggtttctt cctgttcgag gacagaaagt tcgcagatat tggcaacact gtcaagcacc      960
agtaccggtg tcaccgaatc gccgagtggt ccgatatcac caacgcccac ggtgtacccg     1020
gaaccggaat cattgctggc ctgcgagctg gtgccgagga aactgtctct gaacagaaga     1080
aggaggacgt ctctgactac gagaactccc agtacaagga gttcctagtc ccctctccca     1140
acgagaagct ggccagaggt ctgctcatgc tggccgagct gtcttgcaag ggctctctgg     1200
ccactggcga gtactccaag cagaccattg agcttgcccg atccgacccc gagtttgtgg     1260
ttggcttcat tgcccagaac cgacctaagg gcgactctga ggactggctt attctgaccc     1320
ccggggtggg tcttgacgac aagggagacg ctctcggaca gcagtaccga actgttgagg     1380
atgtcatgtc taccggaacg gatatcataa ttgtcggccg aggtctgtac ggccagaacc     1440
gagatcctat tgaggaggcc aagcgatacc agaaggctgg ctgggaggct taccagaaga     1500
ttaactgtta gaggttagac tatggatatg taatttaact gtgtatatag agagcgtgca     1560
agtatggagc gcttgttcag cttgtatgat ggtcagacga cctgtctgat cgagtatgta     1620
tgatactgca caacctgtgt atccgcatga tctgtccaat ggggcatgtt gttgtgtttc     1680
tcgatacgga gatgctgggt acagtgctaa tacg                                 1714

<210>  57
<211>  23
<212>  DNA
<213>  Yarrowia lipolytica

<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  ura3-1 target site

<400>  57
cgctcgagtg ctcaagctcg tgg                                               23

<210>  58
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>   ura3-1F

<400>  58
aatgggacgc cgctcgagtg ctcaagctcg gttt                                   34

<210>  59
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  ura3-1R

<400>  59
tctaaaaccg agcttgagca ctcgagcggc gtcc                                   34

<210>  60
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA encoding Ura3-1 VT domain

<400>  60
cgctcgagtg ctcaagctcg                                                   20

<210>  61
<211>  11631
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF421

<400>  61
taagactatg ataacttcgt ataatgtatg ctatacgaac ggtagcacac tgtacgagta       60
agagcactag ccactgcact caagtgaaac cgttgcccgg gtacgagtat gagtatgtac      120
agtatgttta gtattgtact tggacagtgc ttgtatcgta cattctcaag tgtcaaacat      180
aaatatccgt tgctatatcc tcgcaccacc acgtagctcg ctatatccct gtgttgaatc      240
catccatctt ggattgccaa ttgtgcacac agaaccgggc actcacttcc ccatccacac      300
ttgcggccgc tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc      360
gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg      420
cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc      480
atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat      540
atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt      600
ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg      660
ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt      720
caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc      780
ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat      840
cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt      900
agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc      960
ggccgcagcg atcgcatcca tagcctccgc gaccggctgc agaacagcgg gcagttcggt     1020
ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct     1080
ctcgctgaac tccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg     1140
ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata     1200
tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat     1260
caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag     1320
ttcaggcttt ttggccatgg ttgatgtgtg tttaattcaa gaatgaatat agagaagaga     1380
agaagaaaaa agattcaatt gagccggcga tgcagaccct tatataaatg ttgccttgga     1440
cagacggagc aagcccgccc aaacctacgt tcggtataat atgttaagct ttttaacaca     1500
aaggtttggc ttggggtaac ctgatgtggt gcaaaagacc gggcgttggc gagccattgc     1560
gcgggcgaat ggggccgtga ctcgtctcaa attcgagggc gtgcctcaat tcgtgccccc     1620
gtggcttttt cccgccgttt ccgccccgtt tgcaccactg cagccgcttc tttggttcgg     1680
acaccttgct gcgagctagg tgccttgtgc tacttaaaaa gtggcctccc aacaccaaca     1740
tgacatgagt gcgtgggcca agacacgttg gcggggtcgc agtcggctca atggcccgga     1800
aaaaacgctg ctggagctgg ttcggacgca gtccgccgcg gcgtatggat atccgcaagg     1860
ttccatagcg ccattgccct ccgtcggcgt ctatcccgca acctaccgtt cgtataatgt     1920
atgctatacg aagttatgag cgggcttaag gtttaaacca tcatctaagg gcctcaaaac     1980
tacctcggaa ctgctgcgct gatctggaca ccacagaggt tccgagcact ttaggttgca     2040
ccaaatgtcc caccaggtgc aggcagaaaa cgctggaaca gcgtgtacag tttgtcttaa     2100
caaaaagtga gggcgctgag gtcgagcagg gtggtgtgac ttgttatagc ctttagagct     2160
gcgaaagcgc gtatggattt ggctcatcag gccagattga gggtctgtgg acacatgtca     2220
tgttagtgta cttcaatcgc cccctggata tagccccgac aataggccgt ggcctcattt     2280
ttttgccttc cgcacatttc cattgctcgg tacccacacc ttgcttctcc tgcacttgcc     2340
aaccttaata ctggtttaca ttgaccaaca tcttacaagc ggggggcttg tctagggtat     2400
atataaacag tggctctccc aatcggttgc cagtctcttt tttcctttct ttccccacag     2460
attcgaaatc taaactacac atcacaccat ggacaagaaa tactccatcg gcctggacat     2520
tggaaccaac tctgtcggct gggctgtcat caccgacgag tacaaggtgc cctccaagaa     2580
attcaaggtc ctcggaaaca ccgatcgaca ctccatcaag aaaaacctca ttggtgccct     2640
gttgttcgat tctggcgaga ctgccgaagc taccagactc aagcgaactg ctcggcgacg     2700
ttacacccga cggaagaacc gaatctgcta cctgcaggag atcttttcca acgagatggc     2760
caaggtggac gattcgttct ttcatcgact ggaggaatcc ttcctcgtcg aggaagacaa     2820
gaaacacgag cgtcatccca tctttggcaa cattgtggac gaggttgctt accacgagaa     2880
gtatcctacc atctaccatc tccgaaagaa actcgtcgat tccaccgaca aggcggatct     2940
cagacttatc tacctcgctc tggcacacat gatcaagttt cgaggtcatt tcctcatcga     3000
gggcgatctc aatcccgaca acagcgatgt ggacaagctg ttcattcagc tcgttcagac     3060
ctacaaccag ctgttcgagg aaaaccccat caatgcctcc ggagtcgatg caaaggccat     3120
cttgtctgct cgactctcga agagcagacg actggagaac ctcattgccc aacttcctgg     3180
cgagaaaaag aacggactgt ttggcaacct cattgccctt tctcttggtc tcacacccaa     3240
cttcaagtcc aacttcgatc tggcggagga cgccaagctc cagctgtcca aggacaccta     3300
cgacgatgac ctcgacaacc tgcttgcaca gattggcgat cagtacgccg acctgtttct     3360
cgctgccaag aacctttcgg atgctattct cttgtctgac attctgcgag tcaacaccga     3420
gatcacaaag gctccccttt ctgcctccat gatcaagcga tacgacgagc accatcagga     3480
tctcacactg ctcaaggctc ttgtccgaca gcaactgccc gagaagtaca aggagatctt     3540
tttcgatcag tcgaagaacg gctacgctgg atacatcgac ggcggagcct ctcaggaaga     3600
gttctacaag ttcatcaagc caattctcga gaagatggac ggaaccgagg aactgcttgt     3660
caagctcaat cgagaggatc tgcttcggaa gcaacgaacc ttcgacaacg gcagcattcc     3720
tcatcagatc cacctcggtg agctgcacgc cattcttcga cgtcaggaag acttctaccc     3780
ctttctcaag gacaaccgag agaagatcga gaagattctt acctttcgaa tcccctacta     3840
tgttggtcct cttgccagag gaaactctcg atttgcttgg atgactcgaa agtccgagga     3900
aaccatcact ccctggaact tcgaggaagt cgtggacaag ggtgcctctg cacagtcctt     3960
catcgagcga atgaccaact tcgacaagaa tctgcccaac gagaaggttc ttcccaagca     4020
ttcgctgctc tacgagtact ttacagtcta caacgaactc accaaagtca agtacgttac     4080
cgagggaatg cgaaagcctg ccttcttgtc tggcgaacag aagaaagcca ttgtcgatct     4140
cctgttcaag accaaccgaa aggtcactgt taagcagctc aaggaggact acttcaagaa     4200
aatcgagtgt ttcgacagcg tcgagatttc cggagttgag gaccgattca acgcctcttt     4260
gggcacctat cacgatctgc tcaagattat caaggacaag gattttctcg acaacgagga     4320
aaacgaggac attctggagg acatcgtgct cactcttacc ctgttcgaag atcgggagat     4380
gatcgaggaa cgactcaaga catacgctca cctgttcgac gacaaggtca tgaaacaact     4440
caagcgacgt agatacaccg gctggggaag actttcgcga aagctcatca acggcatcag     4500
agacaagcag tccggaaaga ccattctgga ctttctcaag tccgatggct ttgccaaccg     4560
aaacttcatg cagctcattc acgacgattc tcttaccttc aaggaggaca tccagaaggc     4620
acaagtgtcc ggtcagggcg acagcttgca cgaacatatt gccaacctgg ctggttcgcc     4680
agccatcaag aaaggcattc tccagactgt caaggttgtc gacgagctgg tgaaggtcat     4740
gggacgtcac aagcccgaga acattgtgat cgagatggcc agagagaacc agacaactca     4800
aaagggtcag aaaaactcgc gagagcggat gaagcgaatc gaggaaggca tcaaggagct     4860
gggatcccag attctcaagg agcatcccgt cgagaacact caactgcaga acgagaagct     4920
gtatctctac tatctgcaga atggtcgaga catgtacgtg gatcaggaac tggacatcaa     4980
tcgtctcagc gactacgatg tggaccacat tgtccctcaa tcctttctca aggacgattc     5040
tatcgacaac aaggtcctta cacgatccga caagaacaga ggcaagtcgg acaacgttcc     5100
cagcgaagag gtggtcaaaa agatgaagaa ctactggcga cagctgctca acgccaagct     5160
cattacccag cgaaagttcg acaatcttac caaggccgag cgaggcggtc tgtccgagct     5220
cgacaaggct ggcttcatca agcgtcaact cgtcgagacc agacagatca caaagcacgt     5280
cgcacagatt ctcgattctc ggatgaacac caagtacgac gagaacgaca agctcatccg     5340
agaggtcaag gtgattactc tcaagtccaa actggtctcc gatttccgaa aggactttca     5400
gttctacaag gtgcgagaga tcaacaatta ccaccatgcc cacgatgctt acctcaacgc     5460
cgtcgttggc actgcgctca tcaagaaata ccccaagctc gaaagcgagt tcgtttacgg     5520
cgattacaag gtctacgacg ttcgaaagat gattgccaag tccgaacagg agattggcaa     5580
ggctactgcc aagtacttct tttactccaa catcatgaac tttttcaaga ccgagatcac     5640
cttggccaac ggagagattc gaaagagacc acttatcgag accaacggcg aaactggaga     5700
gatcgtgtgg gacaagggtc gagactttgc aaccgtgcga aaggttctgt cgatgcctca     5760
ggtcaacatc gtcaagaaaa ccgaggttca gactggcgga ttctccaagg agtcgattct     5820
gcccaagcga aactccgaca agctcatcgc tcgaaagaaa gactgggatc ccaagaaata     5880
cggtggcttc gattctccta ccgtcgccta ttccgtgctt gtcgttgcga aggtcgagaa     5940
gggcaagtcc aaaaagctca agtccgtcaa ggagctgctc ggaattacca tcatggagcg     6000
atcgagcttc gagaagaatc ccatcgactt cttggaagcc aagggttaca aggaggtcaa     6060
gaaagacctc attatcaagc tgcccaagta ctctctgttc gaactggaga acggtcgaaa     6120
gcgtatgctc gcctccgctg gcgagctgca gaagggaaac gagcttgcct tgccttcgaa     6180
gtacgtcaac tttctctatc tggcttctca ctacgagaag ctcaagggtt ctcccgagga     6240
caacgaacag aagcaactct tcgttgagca gcacaaacat tacctcgacg agattatcga     6300
gcagatttcc gagttttcga agcgagtcat cctggctgat gccaacttgg acaaggtgct     6360
ctctgcctac aacaagcatc gggacaaacc cattcgagaa caggcggaga acatcattca     6420
cctgtttact cttaccaacc tgggtgctcc tgcagctttc aagtacttcg ataccactat     6480
cgaccgaaag cggtacacat ccaccaagga ggttctcgat gccaccctga ttcaccagtc     6540
catcactggc ctgtacgaga cccgaatcga cctgtctcag cttggtggcg actccagagc     6600
cgatcccaag aaaaagcgaa aggtctaagc ggccgcaagt gtggatgggg aagtgagtgc     6660
ccggttctgt gtgcacaatt ggcaatccaa gatggatgga ttcaacacag ggatatagcg     6720
agctacgtgg tggtgcgagg atatagcaac ggatatttat gtttgacact tgagaatgta     6780
cgatacaagc actgtccaag tacaatacta aacatactgt acatactcat actcgtaccc     6840
gggcaacggt ttcacttgag tgcagtggct agtgctctta ctcgtacagt gtgcaatact     6900
gcgtatcata gtctttgatg tatatcgtat tcattcatgt tagttgcgta cgagccggaa     6960
gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc     7020
gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc     7080
aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact     7140
cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac     7200
ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa     7260
aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg     7320
acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa     7380
gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc     7440
ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac     7500
gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac     7560
cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg     7620
taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt     7680
atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga     7740
cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct     7800
cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga     7860
ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg     7920
ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct     7980
tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt     8040
aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc     8100
tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg     8160
gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag     8220
atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt     8280
tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag     8340
ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt     8400
ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca     8460
tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg     8520
ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat     8580
ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta     8640
tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca     8700
gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct     8760
taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat     8820
cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa     8880
agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt     8940
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa     9000
ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta     9060
gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca     9120
gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct     9180
ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc     9240
acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat     9300
agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc     9360
aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc     9420
cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta     9480
acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg     9540
gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag     9600
gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag     9660
tgaattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga     9720
tggtgtcgat aagcttgata tcgaattcat gtcacacaaa ccgatcttcg cctcaaggaa     9780
acctaattct acatccgaga gactgccgag atccagtcta cactgattaa ttttcgggcc     9840
aataatttaa aaaaatcgtg ttatataata ttatatgtat tatatatata catcatgatg     9900
atactgacag tcatgtccca ttgctaaata gacagactcc atctgccgcc tccaactgat     9960
gttctcaata tttaaggggt catctcgcat tgtttaataa taaacagact ccatctaccg    10020
cctccaaatg atgttctcaa aatatattgt atgaacttat ttttattact tagtattatt    10080
agacaactta cttgctttat gaaaaacact tcctatttag gaaacaattt ataatggcag    10140
ttcgttcatt taacaattta tgtagaataa atgttataaa tgcgtatggg aaatcttaaa    10200
tatggatagc ataaatgata tctgcattgc ctaattcgaa atcaacagca acgaaaaaaa    10260
tcccttgtac aacataaata gtcatcgaga aatatcaact atcaaagaac agctattcac    10320
acgttactat tgagattatt attggacgag aatcacacac tcaactgtct ttctctcttc    10380
tagaaataca ggtacaagta tgtactattc tcattgttca tacttctagt catttcatcc    10440
cacatattcc ttggatttct ctccaatgaa tgacattcta tcttgcaaat tcaacaatta    10500
taataagata taccaaagta gcggtatagt ggcaatcaaa aagcttctct ggtgtgcttc    10560
tcgtatttat ttttattcta atgatccatt aaaggtatat atttatttct tgttatataa    10620
tccttttgtt tattacatgg gctggataca taaaggtatt ttgatttaat tttttgctta    10680
aattcaatcc cccctcgttc agtgtcaact gtaatggtag gaaattacca tacttttgaa    10740
gaagcaaaaa aaatgaaaga aaaaaaaaat cgtatttcca ggttagacgt tccgcagaat    10800
ctagaatgcg gtatgcggta cattgttctt cgaacgtaaa agttgcgctc cctgagatat    10860
tgtacatttt tgcttttaca agtacaagta catcgtacaa ctatgtacta ctgttgatgc    10920
atccacaaca gtttgttttg tttttttttg tttttttttt ttctaatgat tcattaccgc    10980
tatgtatacc tacttgtact tgtagtaagc cgggttattg gcgttcaatt aatcatagac    11040
ttatgaatct gcacggtgtg cgctgcgagt tacttttagc ttatgcatgc tacttgggtg    11100
taatattggg atctgttcgg aaatcaacgg atgctcaatc gataaaaaac aaaaaaaaaa    11160
gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta    11220
tttctagctc taaaaccgag cttgagcact cgagcggcgt cccattcgcc atgccgaagc    11280
atgttgccca gccggcgcca gcgaggaggc tgggaccatg ccggccatta ttttgcgtta    11340
agtttctaat catcacgaaa ttatctatca aaaataacta ggtcccaccg agattcgaac    11400
tcgggacctt aagatttgca atctcacgcg ctaccgctgt gccataggac cgaagttaaa    11460
atttggccaa agaaggacct gggcaccctg gactgtgggt tagggtaata ttccttatgg    11520
agacaatggg ctagggtaaa ttacctaaaa tgggtcgata aagaggggtg ttcccagttg    11580
ggaagtgtaa ttgaagacgg ggtcaaaaaa gaaaatcaaa aaaaatttaa t             11631

<210>  62
<211>  378
<212>  DNA
<213>  Yarrowia lipolytica

<400>  62
gagtatctgt ctgactcgtc attgccgcct ttggagtacg actccaacta tgagtgtgct       60
tggatcactt tgacgataca ttcttcgttg gaggctgtgg gtctgacagc tgcgttttcg      120
gcgcggttgg ccgacaacaa tatcagctgc aacgtcattg ctggctttca tcatgatcac      180
atttttgtcg gcaaaggcga cgcccagaga gccattgacg ttctttctaa tttggaccga      240
tagccgtata gtccagtcta tctataagtt caactaactc gtaactatta ccataacata      300
tacttcactg ccccagataa ggttccgata aaaagttctg cagactaaat ttatttcagt      360
ctcctcttca ccaccaaa                                                    378

<210>  63
<211>  258
<212>  DNA
<213>  Yarrowia lipolytica

<400>  63
tagaggttag actatggata tgtaatttaa ctgtgtatat agagagcgtg caagtatgga       60
gcgcttgttc agcttgtatg atggtcagac gacctgtctg atcgagtatg tatgatactg      120
cacaacctgt gtatccgcat gatctgtcca atggggcatg ttgttgtgtt tctcgatacg      180
gagatgctgg gtacagtgct aatacgttga actacttata cttatatgag gctcgaagaa      240
agctgacttg tgtatgac                                                    258

<210>  64
<211>  648
<212>  DNA
<213>  Artificial sequence

<220>
<223>  URA3 deletion polynucleotide editing template

<400>  64
gaattcgagt atctgtctga ctcgtcattg ccgcctttgg agtacgactc caactatgag       60
tgtgcttgga tcactttgac gatacattct tcgttggagg ctgtgggtct gacagctgcg      120
ttttcggcgc ggttggccga caacaatatc agctgcaacg tcattgctgg ctttcatcat      180
gatcacattt ttgtcggcaa aggcgacgcc cagagagcca ttgacgttct ttctaatttg      240
gaccgatagc cgtatagtcc agtctatcta taagttcaac taactcgtaa ctattaccat      300
aacatatact tcactgcccc agataaggtt ccgataaaaa gttctgcaga ctaaatttat      360
ttcagtctcc tcttcaccac caaatagagg ttagactatg gatatgtaat ttaactgtgt      420
atatagagag cgtgcaagta tggagcgctt gttcagcttg tatgatggtc agacgacctg      480
tctgatcgag tatgtatgat actgcacaac ctgtgtatcc gcatgatctg tccaatgggg      540
catgttgttg tgtttctcga tacggagatg ctgggtacag tgctaatacg ttgaactact      600
tatacttata tgaggctcga agaaagctga cttgtgtatg acaagctt                   648

<210>  65
<211>  3277
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF263

<400>  65
agcttgtcat acacaagtca gctttcttcg agcctcatat aagtataagt agttcaacgt       60
attagcactg tacccagcat ctccgtatcg agaaacacaa caacatgccc cattggacag      120
atcatgcgga tacacaggtt gtgcagtatc atacatactc gatcagacag gtcgtctgac      180
catcatacaa gctgaacaag cgctccatac ttgcacgctc tctatataca cagttaaatt      240
acatatccat agtctaacct ctatttggtg gtgaagagga gactgaaata aatttagtct      300
gcagaacttt ttatcggaac cttatctggg gcagtgaagt atatgttatg gtaatagtta      360
cgagttagtt gaacttatag atagactgga ctatacggct atcggtccaa attagaaaga      420
acgtcaatgg ctctctgggc gtcgcctttg ccgacaaaaa tgtgatcatg atgaaagcca      480
gcaatgacgt tgcagctgat attgttgtcg gccaaccgcg ccgaaaacgc agctgtcaga      540
cccacagcct ccaacgaaga atgtatcgtc aaagtgatcc aagcacactc atagttggag      600
tcgtactcca aaggcggcaa tgacgagtca gacagatact cgaattcgta atcatggtca      660
tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga      720
agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg      780
cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc      840
caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac      900
tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata      960
cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     1020
aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     1080
gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     1140
agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     1200
cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     1260
cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     1320
ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     1380
gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     1440
tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     1500
acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     1560
tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     1620
attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     1680
gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc     1740
ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag     1800
taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt     1860
ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag     1920
ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca     1980
gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact     2040
ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca     2100
gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg     2160
tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc     2220
atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg     2280
gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca     2340
tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt     2400
atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc     2460
agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc     2520
ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca     2580
tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa     2640
aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat     2700
tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa     2760
aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa     2820
accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc     2880
gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca     2940
gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt     3000
ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac     3060
catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat     3120
tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta     3180
cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt     3240
tcccagtcac gacgttgtaa aacgacggcc agtgcca                              3277

<210>  66
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>   HY007

<400>  66
cgggcagtga gcgcaacgca attaat                                            26

<210>  67
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligo 297

<400>  67
ggggaattct gcaaggcgat taagttggg                                         29

<210>  68
<211>  873
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EcoRI flanked URA3 deletion template

<400>  68
cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt       60
tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      120
caggaaacag ctatgaccat gattacgaat tcgagtatct gtctgactcg tcattgccgc      180
ctttggagta cgactccaac tatgagtgtg cttggatcac tttgacgata cattcttcgt      240
tggaggctgt gggtctgaca gctgcgtttt cggcgcggtt ggccgacaac aatatcagct      300
gcaacgtcat tgctggcttt catcatgatc acatttttgt cggcaaaggc gacgcccaga      360
gagccattga cgttctttct aatttggacc gatagccgta tagtccagtc tatctataag      420
ttcaactaac tcgtaactat taccataaca tatacttcac tgccccagat aaggttccga      480
taaaaagttc tgcagactaa atttatttca gtctcctctt caccaccaaa tagaggttag      540
actatggata tgtaatttaa ctgtgtatat agagagcgtg caagtatgga gcgcttgttc      600
agcttgtatg atggtcagac gacctgtctg atcgagtatg tatgatactg cacaacctgt      660
gtatccgcat gatctgtcca atggggcatg ttgttgtgtt tctcgatacg gagatgctgg      720
gtacagtgct aatacgttga actacttata cttatatgag gctcgaagaa agctgacttg      780
tgtatgacaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc      840
gttacccaac ttaatcgcct tgcagaattc ccc                                   873

<210>  69
<211>  12349
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRF437

<400>  69
aattctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa       60
acgacggcca gtgccaagct tgtcatacac aagtcagctt tcttcgagcc tcatataagt      120
ataagtagtt caacgtatta gcactgtacc cagcatctcc gtatcgagaa acacaacaac      180
atgccccatt ggacagatca tgcggataca caggttgtgc agtatcatac atactcgatc      240
agacaggtcg tctgaccatc atacaagctg aacaagcgct ccatacttgc acgctctcta      300
tatacacagt taaattacat atccatagtc taacctctat ttggtggtga agaggagact      360
gaaataaatt tagtctgcag aactttttat cggaacctta tctggggcag tgaagtatat      420
gttatggtaa tagttacgag ttagttgaac ttatagatag actggactat acggctatcg      480
gtccaaatta gaaagaacgt caatggctct ctgggcgtcg cctttgccga caaaaatgtg      540
atcatgatga aagccagcaa tgacgttgca gctgatattg ttgtcggcca accgcgccga      600
aaacgcagct gtcagaccca cagcctccaa cgaagaatgt atcgtcaaag tgatccaagc      660
acactcatag ttggagtcgt actccaaagg cggcaatgac gagtcagaca gatactcgaa      720
ttcatgtcac acaaaccgat cttcgcctca aggaaaccta attctacatc cgagagactg      780
ccgagatcca gtctacactg attaattttc gggccaataa tttaaaaaaa tcgtgttata      840
taatattata tgtattatat atatacatca tgatgatact gacagtcatg tcccattgct      900
aaatagacag actccatctg ccgcctccaa ctgatgttct caatatttaa ggggtcatct      960
cgcattgttt aataataaac agactccatc taccgcctcc aaatgatgtt ctcaaaatat     1020
attgtatgaa cttattttta ttacttagta ttattagaca acttacttgc tttatgaaaa     1080
acacttccta tttaggaaac aatttataat ggcagttcgt tcatttaaca atttatgtag     1140
aataaatgtt ataaatgcgt atgggaaatc ttaaatatgg atagcataaa tgatatctgc     1200
attgcctaat tcgaaatcaa cagcaacgaa aaaaatccct tgtacaacat aaatagtcat     1260
cgagaaatat caactatcaa agaacagcta ttcacacgtt actattgaga ttattattgg     1320
acgagaatca cacactcaac tgtctttctc tcttctagaa atacaggtac aagtatgtac     1380
tattctcatt gttcatactt ctagtcattt catcccacat attccttgga tttctctcca     1440
atgaatgaca ttctatcttg caaattcaac aattataata agatatacca aagtagcggt     1500
atagtggcaa tcaaaaagct tctctggtgt gcttctcgta tttattttta ttctaatgat     1560
ccattaaagg tatatattta tttcttgtta tataatcctt ttgtttatta catgggctgg     1620
atacataaag gtattttgat ttaatttttt gcttaaattc aatcccccct cgttcagtgt     1680
caactgtaat ggtaggaaat taccatactt ttgaagaagc aaaaaaaatg aaagaaaaaa     1740
aaaatcgtat ttccaggtta gacgttccgc agaatctaga atgcggtatg cggtacattg     1800
ttcttcgaac gtaaaagttg cgctccctga gatattgtac atttttgctt ttacaagtac     1860
aagtacatcg tacaactatg tactactgtt gatgcatcca caacagtttg ttttgttttt     1920
ttttgttttt tttttttcta atgattcatt accgctatgt atacctactt gtacttgtag     1980
taagccgggt tattggcgtt caattaatca tagacttatg aatctgcacg gtgtgcgctg     2040
cgagttactt ttagcttatg catgctactt gggtgtaata ttgggatctg ttcggaaatc     2100
aacggatgct caatcgataa aaaacaaaaa aaaaagcacc gactcggtgc cactttttca     2160
agttgataac ggactagcct tattttaact tgctatttct agctctaaaa ccgagcttga     2220
gcactcgagc ggcgtcccat tcgccatgcc gaagcatgtt gcccagccgg cgccagcgag     2280
gaggctggga ccatgccggc cattattttg cgttaagttt ctaatcatca cgaaattatc     2340
tatcaaaaat aactaggtcc caccgagatt cgaactcggg accttaagat ttgcaatctc     2400
acgcgctacc gctgtgccat aggaccgaag ttaaaatttg gccaaagaag gacctgggca     2460
ccctggactg tgggttaggg taatattcct tatggagaca atgggctagg gtaaattacc     2520
taaaatgggt cgataaagag gggtgttccc agttgggaag tgtaattgaa gacggggtca     2580
aaaaagaaaa tcaaaaaaaa tttaattaag actatgataa cttcgtataa tgtatgctat     2640
acgaacggta gcacactgta cgagtaagag cactagccac tgcactcaag tgaaaccgtt     2700
gcccgggtac gagtatgagt atgtacagta tgtttagtat tgtacttgga cagtgcttgt     2760
atcgtacatt ctcaagtgtc aaacataaat atccgttgct atatcctcgc accaccacgt     2820
agctcgctat atccctgtgt tgaatccatc catcttggat tgccaattgt gcacacagaa     2880
ccgggcactc acttccccat ccacacttgc ggccgctatt cctttgccct cggacgagtg     2940
ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc     3000
gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg     3060
tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag     3120
agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc     3180
ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag     3240
aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg     3300
accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg     3360
aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg     3420
acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca     3480
atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg     3540
ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc     3600
ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca     3660
cggcgggaga tgcaataggt caggctctcg ctgaactccc caatgtcaag cacttccgga     3720
atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg     3780
gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga     3840
gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga     3900
aacttctcga cagacgtcgc ggtgagttca ggctttttgg ccatggttga tgtgtgttta     3960
attcaagaat gaatatagag aagagaagaa gaaaaaagat tcaattgagc cggcgatgca     4020
gacccttata taaatgttgc cttggacaga cggagcaagc ccgcccaaac ctacgttcgg     4080
tataatatgt taagcttttt aacacaaagg tttggcttgg ggtaacctga tgtggtgcaa     4140
aagaccgggc gttggcgagc cattgcgcgg gcgaatgggg ccgtgactcg tctcaaattc     4200
gagggcgtgc ctcaattcgt gcccccgtgg ctttttcccg ccgtttccgc cccgtttgca     4260
ccactgcagc cgcttctttg gttcggacac cttgctgcga gctaggtgcc ttgtgctact     4320
taaaaagtgg cctcccaaca ccaacatgac atgagtgcgt gggccaagac acgttggcgg     4380
ggtcgcagtc ggctcaatgg cccggaaaaa acgctgctgg agctggttcg gacgcagtcc     4440
gccgcggcgt atggatatcc gcaaggttcc atagcgccat tgccctccgt cggcgtctat     4500
cccgcaacct accgttcgta taatgtatgc tatacgaagt tatgagcggg cttaaggttt     4560
aaaccatcat ctaagggcct caaaactacc tcggaactgc tgcgctgatc tggacaccac     4620
agaggttccg agcactttag gttgcaccaa atgtcccacc aggtgcaggc agaaaacgct     4680
ggaacagcgt gtacagtttg tcttaacaaa aagtgagggc gctgaggtcg agcagggtgg     4740
tgtgacttgt tatagccttt agagctgcga aagcgcgtat ggatttggct catcaggcca     4800
gattgagggt ctgtggacac atgtcatgtt agtgtacttc aatcgccccc tggatatagc     4860
cccgacaata ggccgtggcc tcattttttt gccttccgca catttccatt gctcggtacc     4920
cacaccttgc ttctcctgca cttgccaacc ttaatactgg tttacattga ccaacatctt     4980
acaagcgggg ggcttgtcta gggtatatat aaacagtggc tctcccaatc ggttgccagt     5040
ctcttttttc ctttctttcc ccacagattc gaaatctaaa ctacacatca caccatggac     5100
aagaaatact ccatcggcct ggacattgga accaactctg tcggctgggc tgtcatcacc     5160
gacgagtaca aggtgccctc caagaaattc aaggtcctcg gaaacaccga tcgacactcc     5220
atcaagaaaa acctcattgg tgccctgttg ttcgattctg gcgagactgc cgaagctacc     5280
agactcaagc gaactgctcg gcgacgttac acccgacgga agaaccgaat ctgctacctg     5340
caggagatct tttccaacga gatggccaag gtggacgatt cgttctttca tcgactggag     5400
gaatccttcc tcgtcgagga agacaagaaa cacgagcgtc atcccatctt tggcaacatt     5460
gtggacgagg ttgcttacca cgagaagtat cctaccatct accatctccg aaagaaactc     5520
gtcgattcca ccgacaaggc ggatctcaga cttatctacc tcgctctggc acacatgatc     5580
aagtttcgag gtcatttcct catcgagggc gatctcaatc ccgacaacag cgatgtggac     5640
aagctgttca ttcagctcgt tcagacctac aaccagctgt tcgaggaaaa ccccatcaat     5700
gcctccggag tcgatgcaaa ggccatcttg tctgctcgac tctcgaagag cagacgactg     5760
gagaacctca ttgcccaact tcctggcgag aaaaagaacg gactgtttgg caacctcatt     5820
gccctttctc ttggtctcac acccaacttc aagtccaact tcgatctggc ggaggacgcc     5880
aagctccagc tgtccaagga cacctacgac gatgacctcg acaacctgct tgcacagatt     5940
ggcgatcagt acgccgacct gtttctcgct gccaagaacc tttcggatgc tattctcttg     6000
tctgacattc tgcgagtcaa caccgagatc acaaaggctc ccctttctgc ctccatgatc     6060
aagcgatacg acgagcacca tcaggatctc acactgctca aggctcttgt ccgacagcaa     6120
ctgcccgaga agtacaagga gatctttttc gatcagtcga agaacggcta cgctggatac     6180
atcgacggcg gagcctctca ggaagagttc tacaagttca tcaagccaat tctcgagaag     6240
atggacggaa ccgaggaact gcttgtcaag ctcaatcgag aggatctgct tcggaagcaa     6300
cgaaccttcg acaacggcag cattcctcat cagatccacc tcggtgagct gcacgccatt     6360
cttcgacgtc aggaagactt ctaccccttt ctcaaggaca accgagagaa gatcgagaag     6420
attcttacct ttcgaatccc ctactatgtt ggtcctcttg ccagaggaaa ctctcgattt     6480
gcttggatga ctcgaaagtc cgaggaaacc atcactccct ggaacttcga ggaagtcgtg     6540
gacaagggtg cctctgcaca gtccttcatc gagcgaatga ccaacttcga caagaatctg     6600
cccaacgaga aggttcttcc caagcattcg ctgctctacg agtactttac agtctacaac     6660
gaactcacca aagtcaagta cgttaccgag ggaatgcgaa agcctgcctt cttgtctggc     6720
gaacagaaga aagccattgt cgatctcctg ttcaagacca accgaaaggt cactgttaag     6780
cagctcaagg aggactactt caagaaaatc gagtgtttcg acagcgtcga gatttccgga     6840
gttgaggacc gattcaacgc ctctttgggc acctatcacg atctgctcaa gattatcaag     6900
gacaaggatt ttctcgacaa cgaggaaaac gaggacattc tggaggacat cgtgctcact     6960
cttaccctgt tcgaagatcg ggagatgatc gaggaacgac tcaagacata cgctcacctg     7020
ttcgacgaca aggtcatgaa acaactcaag cgacgtagat acaccggctg gggaagactt     7080
tcgcgaaagc tcatcaacgg catcagagac aagcagtccg gaaagaccat tctggacttt     7140
ctcaagtccg atggctttgc caaccgaaac ttcatgcagc tcattcacga cgattctctt     7200
accttcaagg aggacatcca gaaggcacaa gtgtccggtc agggcgacag cttgcacgaa     7260
catattgcca acctggctgg ttcgccagcc atcaagaaag gcattctcca gactgtcaag     7320
gttgtcgacg agctggtgaa ggtcatggga cgtcacaagc ccgagaacat tgtgatcgag     7380
atggccagag agaaccagac aactcaaaag ggtcagaaaa actcgcgaga gcggatgaag     7440
cgaatcgagg aaggcatcaa ggagctggga tcccagattc tcaaggagca tcccgtcgag     7500
aacactcaac tgcagaacga gaagctgtat ctctactatc tgcagaatgg tcgagacatg     7560
tacgtggatc aggaactgga catcaatcgt ctcagcgact acgatgtgga ccacattgtc     7620
cctcaatcct ttctcaagga cgattctatc gacaacaagg tccttacacg atccgacaag     7680
aacagaggca agtcggacaa cgttcccagc gaagaggtgg tcaaaaagat gaagaactac     7740
tggcgacagc tgctcaacgc caagctcatt acccagcgaa agttcgacaa tcttaccaag     7800
gccgagcgag gcggtctgtc cgagctcgac aaggctggct tcatcaagcg tcaactcgtc     7860
gagaccagac agatcacaaa gcacgtcgca cagattctcg attctcggat gaacaccaag     7920
tacgacgaga acgacaagct catccgagag gtcaaggtga ttactctcaa gtccaaactg     7980
gtctccgatt tccgaaagga ctttcagttc tacaaggtgc gagagatcaa caattaccac     8040
catgcccacg atgcttacct caacgccgtc gttggcactg cgctcatcaa gaaatacccc     8100
aagctcgaaa gcgagttcgt ttacggcgat tacaaggtct acgacgttcg aaagatgatt     8160
gccaagtccg aacaggagat tggcaaggct actgccaagt acttctttta ctccaacatc     8220
atgaactttt tcaagaccga gatcaccttg gccaacggag agattcgaaa gagaccactt     8280
atcgagacca acggcgaaac tggagagatc gtgtgggaca agggtcgaga ctttgcaacc     8340
gtgcgaaagg ttctgtcgat gcctcaggtc aacatcgtca agaaaaccga ggttcagact     8400
ggcggattct ccaaggagtc gattctgccc aagcgaaact ccgacaagct catcgctcga     8460
aagaaagact gggatcccaa gaaatacggt ggcttcgatt ctcctaccgt cgcctattcc     8520
gtgcttgtcg ttgcgaaggt cgagaagggc aagtccaaaa agctcaagtc cgtcaaggag     8580
ctgctcggaa ttaccatcat ggagcgatcg agcttcgaga agaatcccat cgacttcttg     8640
gaagccaagg gttacaagga ggtcaagaaa gacctcatta tcaagctgcc caagtactct     8700
ctgttcgaac tggagaacgg tcgaaagcgt atgctcgcct ccgctggcga gctgcagaag     8760
ggaaacgagc ttgccttgcc ttcgaagtac gtcaactttc tctatctggc ttctcactac     8820
gagaagctca agggttctcc cgaggacaac gaacagaagc aactcttcgt tgagcagcac     8880
aaacattacc tcgacgagat tatcgagcag atttccgagt tttcgaagcg agtcatcctg     8940
gctgatgcca acttggacaa ggtgctctct gcctacaaca agcatcggga caaacccatt     9000
cgagaacagg cggagaacat cattcacctg tttactctta ccaacctggg tgctcctgca     9060
gctttcaagt acttcgatac cactatcgac cgaaagcggt acacatccac caaggaggtt     9120
ctcgatgcca ccctgattca ccagtccatc actggcctgt acgagacccg aatcgacctg     9180
tctcagcttg gtggcgactc cagagccgat cccaagaaaa agcgaaaggt ctaagcggcc     9240
gcaagtgtgg atggggaagt gagtgcccgg ttctgtgtgc acaattggca atccaagatg     9300
gatggattca acacagggat atagcgagct acgtggtggt gcgaggatat agcaacggat     9360
atttatgttt gacacttgag aatgtacgat acaagcactg tccaagtaca atactaaaca     9420
tactgtacat actcatactc gtacccgggc aacggtttca cttgagtgca gtggctagtg     9480
ctcttactcg tacagtgtgc aatactgcgt atcatagtct ttgatgtata tcgtattcat     9540
tcatgttagt tgcgtacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt     9600
gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc     9660
gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg     9720
ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt     9780
atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa     9840
gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc     9900
gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag     9960
gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt    10020
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg    10080
aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg    10140
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg    10200
taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac    10260
tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg    10320
gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt    10380
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg    10440
tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc    10500
tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt    10560
ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt    10620
taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag    10680
tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt    10740
cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc    10800
gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc    10860
cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg    10920
ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac    10980
aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg    11040
atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc    11100
tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact    11160
gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc    11220
aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat    11280
acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc    11340
ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac    11400
tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa    11460
aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact    11520
catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg    11580
atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg    11640
aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac    11700
gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc    11760
ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt    11820
agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg    11880
ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac    11940
gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta    12000
ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat    12060
ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt ccattcgcca    12120
ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag    12180
ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag    12240
tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg    12300
ggtaccgggc cccccctcga ggtcgatggt gtcgataagc ttgatatcg                12349

<210>  70
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  oligo 308

<400>  70
cgtattagca ctgtacccag                                                   20

<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligo 309

<400>  71
catgacatgt gtccacagac                                                   20

<210>  72
<211>  859
<212>  DNA
<213>  Artificial sequence

<220>
<223>  URA3 locus deletion PCR product

<400>  72
catgacatgt gtccacagac cctcaatctg gcctgatgag ccaaatccat acgcgctttc       60
gcagctctaa aggctataac aagtcacacc accctgctcg acctcagcgc cctcactttt      120
tgttaagaca aactgtacac gctgttccag cgttttctgc ctgcacctgg tgggacattt      180
ggtgcaacct aaagtgctcg gaacctctgt ggtgtccaga tcagcgcagc agttccgagg      240
tagttttgag gcccttagat gatggtttaa acgtcgacga gtatctgtct gactcgtcat      300
tgccgccttt ggagtacgac tccaactatg agtgtgcttg gatcactttg acgatacatt      360
cttcgttgga ggctgtgggt ctgacagctg cgttttcggc gcggttggcc gacaacaata      420
tcagctgcaa cgtcattgct ggctttcatc atgatcacat ttttgtcggc aaaggcgacg      480
cccagagagc cattgacgtt ctttctaatt tggaccgata gccgtatagt ccagtctatc      540
tataagttca actaactcgt aactattacc ataacatata cttcactgcc ccagataagg      600
ttccgataaa aagttctgca gactaaattt atttcagtct cctcttcacc accaaaaggt      660
tagactatgg atatgtaatt taactgtgta tatagagagc gtgcaagtat ggagcgcttg      720
ttcagcttgt atgatggtca gacgacctgt ctgatcgagt atgtatgata ctgcacaacc      780
tgtgtatccg catgatctgt ccaatggggc atgttgttgt gtttctcgat acggagatgc      840
tgggtacagt gctaatacg                                                   859

