                         SEQUENCE LISTING

<110>  CITY OF HOPE
 
<120>  CHIMERIC ANTIGEN RECEPTORS CONTAINING A CHLOROTOXIN DOMAIN

<130>  40056-0024WO1

<140>  PCT/US2016/056901
<141>  2016-10-13

<150>  US 62/241,021
<151>  2015-10-13

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  36
<212>  PRT
<213>  Leiurus quinquestriatus

<400>  1

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Lys Arg Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg 
        35      


<210>  2
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  2

Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly 
1               5                   10  


<210>  3
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated IgG4 hinge

<400>  3

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  4
<211>  12
<212>  PRT
<213>  Homo sapiens

<400>  4

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  5
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated IgG4 hinge +linker

<400>  5

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Gly Gly Ser Gly 
            20          


<210>  6
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  6

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  7
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  7

Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
1               5                   10                  15      


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            20                  25                  30          


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45              


<210>  8
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  8

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  9
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human chimeric IgG4 HL-Ch3 with mutation

<400>  9

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser 
1               5                   10                  15      


Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
            20                  25                  30          


Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr 
        35                  40                  45              


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
    50                  55                  60                  


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
65                  70                  75                  80  


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys 
                85                  90                  95      


Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
            100                 105                 110         


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
        115                 120                 125             


Lys 
    


<210>  10
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated IgG4

<400>  10

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Gln Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  11
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated IgG4

<400>  11

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Gln Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  12
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  12

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
1               5                   10                  15      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            100                 105         


<210>  13
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  13

Leu Cys Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu 
1               5                   10                  15      


Thr Ala Leu Phe Leu 
            20      


<210>  14
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  14

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  15
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated human CD28

<400>  15

Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              


<210>  16
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile 
1               5                   10                  15      


Gly Leu Gly Ile Phe Phe 
            20          


<210>  17
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  17

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  18
<211>  23
<212>  PRT
<213>  Homo sapiens

<400>  18

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr 
            20              


<210>  19
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  19

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  20
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  20

Ile Ile Ser Phe Phe Leu Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu 
1               5                   10                  15      


Leu Phe Phe Leu Thr Leu Arg Phe Ser Val Val 
            20                  25          


<210>  21
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  22
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  22

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  23
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated CD28

<400>  23

Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  24
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  24

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  25
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  25

Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His 
1               5                   10                  15      


Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln 
            20                  25                  30          


Ala Asp Ala His Ser Thr Leu Ala Lys Ile 
        35                  40          


<210>  26
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD28tm-CD28-zeta including signal

<400>  26

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
65                  70                  75                  80  


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                85                  90                  95      


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
            100                 105                 110         


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        115                 120                 125             


Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
    130                 135                 140                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
145                 150                 155                 160 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
                165                 170                 175     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            180                 185                 190         


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        195                 200                 205             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    210                 215                 220                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
225                 230                 235                 240 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
                245                 250                 255     


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            260                 265                 270         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
        275                 280                 285             


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
    290                 295                 300                 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
305                 310                 315                 320 


Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
                325                 330                 335     


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
            340                 345                 350         


Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
        355                 360                 365             


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
    370                 375                 380                 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
385                 390                 395                 400 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
                405                 410                 415     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            420                 425                 430         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        435                 440                 445             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    450                 455                 460                 


Met Gln Ala Leu Pro Pro Arg 
465                 470     


<210>  27
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD28tm-CD28-zeta including signal

<400>  27

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly 
65                  70                  75                  80  


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
                85                  90                  95      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            100                 105                 110         


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        115                 120                 125             


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    130                 135                 140                 


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
145                 150                 155                 160 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                165                 170                 175     


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu 
            180                 185                 190         


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
        195                 200                 205             


Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His 
    210                 215                 220                 


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys 
225                 230                 235                 240 


His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
                245                 250                 255     


Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
            260                 265                 270         


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
        275                 280                 285             


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
    290                 295                 300                 


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
305                 310                 315                 320 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
                325                 330                 335     


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
            340                 345                 350         


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
        355                 360                 365             


Pro Pro Arg 
    370     


<210>  28
<211>  287
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h- CD28tm-CD28-zeta including signal

<400>  28

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro 
    50                  55                  60                  


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
65                  70                  75                  80  


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
                85                  90                  95      


Gly Leu Asp Phe Ala Cys Asp Met Phe Trp Val Leu Val Val Val Gly 
            100                 105                 110         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        115                 120                 125             


Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met 
    130                 135                 140                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
145                 150                 155                 160 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Arg 
                165                 170                 175     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            180                 185                 190         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        195                 200                 205             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    210                 215                 220                 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
225                 230                 235                 240 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
                245                 250                 255     


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
            260                 265                 270         


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        275                 280                 285         


<210>  29
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(hinge)-CD28tm-CD28-zeta including signal

<400>  29

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly 
65                  70                  75                  80  


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                85                  90                  95      


Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn 
            100                 105                 110         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        115                 120                 125             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Arg Val 
    130                 135                 140                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
145                 150                 155                 160 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                165                 170                 175     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            180                 185                 190         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        195                 200                 205             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    210                 215                 220                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
225                 230                 235                 240 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250                 


<210>  30
<211>  252
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-CD28-zeta including signal

<400>  30

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly 
    50                  55                  60                  


Gly Gly Ser Gly Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
65                  70                  75                  80  


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                85                  90                  95      


Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
            100                 105                 110         


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
        115                 120                 125             


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe 
    130                 135                 140                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
145                 150                 155                 160 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                165                 170                 175     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            180                 185                 190         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        195                 200                 205             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    210                 215                 220                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
225                 230                 235                 240 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250         


<210>  31
<211>  516
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD28tm-CD28-4-1BBzeta including signal

<400>  31

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
65                  70                  75                  80  


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                85                  90                  95      


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
            100                 105                 110         


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        115                 120                 125             


Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
    130                 135                 140                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
145                 150                 155                 160 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
                165                 170                 175     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            180                 185                 190         


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        195                 200                 205             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    210                 215                 220                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
225                 230                 235                 240 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
                245                 250                 255     


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            260                 265                 270         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
        275                 280                 285             


Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
    290                 295                 300                 


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
305                 310                 315                 320 


Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
                325                 330                 335     


Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
            340                 345                 350         


Ala Tyr Arg Ser Gly Gly Gly Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
        355                 360                 365             


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
    370                 375                 380                 


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
385                 390                 395                 400 


Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
                405                 410                 415     


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
            420                 425                 430         


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
        435                 440                 445             


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
    450                 455                 460                 


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
465                 470                 475                 480 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
                485                 490                 495     


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
            500                 505                 510         


Leu Pro Pro Arg 
        515     


<210>  32
<211>  416
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD28tm-CD28-4-1BBzeta including 
       sequence

<400>  32

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly 
65                  70                  75                  80  


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
                85                  90                  95      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            100                 105                 110         


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        115                 120                 125             


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    130                 135                 140                 


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
145                 150                 155                 160 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                165                 170                 175     


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu 
            180                 185                 190         


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
        195                 200                 205             


Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His 
    210                 215                 220                 


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys 
225                 230                 235                 240 


His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
                245                 250                 255     


Gly Gly Gly Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            260                 265                 270         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        275                 280                 285             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly 
    290                 295                 300                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
305                 310                 315                 320 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                325                 330                 335     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            340                 345                 350         


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
        355                 360                 365             


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
    370                 375                 380                 


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
385                 390                 395                 400 


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                405                 410                 415     


<210>  33
<211>  332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h-CD28tm-CD28-4-1BB-zeta including sequence

<400>  33

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro 
    50                  55                  60                  


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
65                  70                  75                  80  


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
                85                  90                  95      


Gly Leu Asp Phe Ala Cys Asp Met Phe Trp Val Leu Val Val Val Gly 
            100                 105                 110         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        115                 120                 125             


Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met 
    130                 135                 140                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
145                 150                 155                 160 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Lys 
                165                 170                 175     


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            180                 185                 190         


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        195                 200                 205             


Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe 
    210                 215                 220                 


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
225                 230                 235                 240 


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
                245                 250                 255     


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
            260                 265                 270         


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
        275                 280                 285             


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
    290                 295                 300                 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
305                 310                 315                 320 


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                325                 330         


<210>  34
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric LTX-IgG4(hinge)-CD28tm-CD28-4-1BBzeta including sequence

<400>  34

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly 
65                  70                  75                  80  


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                85                  90                  95      


Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn 
            100                 105                 110         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        115                 120                 125             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Lys Arg 
    130                 135                 140                 


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
145                 150                 155                 160 


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
                165                 170                 175     


Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser 
            180                 185                 190         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        195                 200                 205             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    210                 215                 220                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
225                 230                 235                 240 


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
                245                 250                 255     


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
            260                 265                 270         


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
        275                 280                 285             


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    290                 295                 


<210>  35
<211>  297
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-CD28-4-1BB-zeta including sequence

<400>  35

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly 
    50                  55                  60                  


Gly Gly Ser Gly Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
65                  70                  75                  80  


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                85                  90                  95      


Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
            100                 105                 110         


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
        115                 120                 125             


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Lys Arg Gly Arg 
    130                 135                 140                 


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
145                 150                 155                 160 


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
                165                 170                 175     


Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser 
            180                 185                 190         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        195                 200                 205             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    210                 215                 220                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
225                 230                 235                 240 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
                245                 250                 255     


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
            260                 265                 270         


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
        275                 280                 285             


Leu His Met Gln Ala Leu Pro Pro Arg 
    290                 295         


<210>  36
<211>  466
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD4tm-4-1BB-zeta including sequence

<400>  36

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val 
65                  70                  75                  80  


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                85                  90                  95      


Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu 
            100                 105                 110         


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        115                 120                 125             


Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr Tyr Arg Val Val Ser 
    130                 135                 140                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
145                 150                 155                 160 


Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile 
                165                 170                 175     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            180                 185                 190         


Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        195                 200                 205             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    210                 215                 220                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
225                 230                 235                 240 


Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg 
                245                 250                 255     


Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            260                 265                 270         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met 
        275                 280                 285             


Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly 
    290                 295                 300                 


Leu Gly Ile Phe Phe Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
305                 310                 315                 320 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                325                 330                 335     


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly 
            340                 345                 350         


Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
        355                 360                 365             


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
    370                 375                 380                 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
385                 390                 395                 400 


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                405                 410                 415     


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            420                 425                 430         


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        435                 440                 445             


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    450                 455                 460                 


Pro Arg 
465     


<210>  37
<211>  366
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD4tm-4-1BB-zeta including sequence

<400>  37

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly 
65                  70                  75                  80  


Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
                85                  90                  95      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            100                 105                 110         


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        115                 120                 125             


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    130                 135                 140                 


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
145                 150                 155                 160 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                165                 170                 175     


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Ala Leu Ile Val 
            180                 185                 190         


Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe 
        195                 200                 205             


Phe Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
    210                 215                 220                 


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
225                 230                 235                 240 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val 
                245                 250                 255     


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
            260                 265                 270         


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
        275                 280                 285             


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
    290                 295                 300                 


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
305                 310                 315                 320 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
                325                 330                 335     


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
            340                 345                 350         


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 365     


<210>  38
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h- CD28tm-4-1BB-zeta including sequence

<400>  38

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro 
    50                  55                  60                  


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
65                  70                  75                  80  


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
                85                  90                  95      


Gly Leu Asp Phe Ala Cys Asp Met Phe Trp Val Leu Val Val Val Gly 
            100                 105                 110         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        115                 120                 125             


Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
    130                 135                 140                 


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
145                 150                 155                 160 


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly 
                165                 170                 175     


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
            180                 185                 190         


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
        195                 200                 205             


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
    210                 215                 220                 


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
225                 230                 235                 240 


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
                245                 250                 255     


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
            260                 265                 270         


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        275                 280                 285             


<210>  39
<211>  255
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(hinge)-CD28tm-4-1BB-zeta including sequence

<400>  39

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro 
    50                  55                  60                  


Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly Gly 
65                  70                  75                  80  


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                85                  90                  95      


Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
            100                 105                 110         


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
        115                 120                 125             


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg 
    130                 135                 140                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
145                 150                 155                 160 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                165                 170                 175     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            180                 185                 190         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        195                 200                 205             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    210                 215                 220                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
225                 230                 235                 240 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250                 255 


<210>  40
<211>  253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-4-1BB-zeta including sequence

<400>  40

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly 
    50                  55                  60                  


Gly Gly Ser Gly Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
65                  70                  75                  80  


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                85                  90                  95      


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            100                 105                 110         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        115                 120                 125             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys 
    130                 135                 140                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
145                 150                 155                 160 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                165                 170                 175     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            180                 185                 190         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        195                 200                 205             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    210                 215                 220                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
225                 230                 235                 240 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                245                 250             


<210>  41
<211>  449
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD28tm-CD28-zeta excluding signal

<400>  41

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    50                  55                  60                  


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
65                  70                  75                  80  


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
                85                  90                  95      


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            100                 105                 110         


Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        115                 120                 125             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    130                 135                 140                 


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
145                 150                 155                 160 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met 
                165                 170                 175     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            180                 185                 190         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        195                 200                 205             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    210                 215                 220                 


Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val 
225                 230                 235                 240 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                245                 250                 255     


Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val 
            260                 265                 270         


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
        275                 280                 285             


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp 
    290                 295                 300                 


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
305                 310                 315                 320 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly 
                325                 330                 335     


Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
            340                 345                 350         


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        355                 360                 365             


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    370                 375                 380                 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
385                 390                 395                 400 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                405                 410                 415     


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            420                 425                 430         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        435                 440                 445             


Arg 
    


<210>  42
<211>  349
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD28tm-CD28-zeta excluding signal

<400>  42

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro 
    50                  55                  60                  


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
65                  70                  75                  80  


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                85                  90                  95      


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            100                 105                 110         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
        115                 120                 125             


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
    130                 135                 140                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
145                 150                 155                 160 


Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
                165                 170                 175     


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            180                 185                 190         


Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met 
        195                 200                 205             


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    210                 215                 220                 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys 
225                 230                 235                 240 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
                245                 250                 255     


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            260                 265                 270         


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        275                 280                 285             


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    290                 295                 300                 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
305                 310                 315                 320 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                325                 330                 335     


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            340                 345                 


<210>  43
<211>  265
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h- CD28tm-CD28-zeta excluding signal

<400>  43

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        35                  40                  45              


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    50                  55                  60                  


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
65                  70                  75                  80  


Asp Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
                85                  90                  95      


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
            100                 105                 110         


Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
        115                 120                 125             


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
    130                 135                 140                 


Phe Ala Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser 
145                 150                 155                 160 


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
                165                 170                 175     


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
            180                 185                 190         


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
        195                 200                 205             


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
    210                 215                 220                 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
225                 230                 235                 240 


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
                245                 250                 255     


Leu His Met Gln Ala Leu Pro Pro Arg 
            260                 265 


<210>  44
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric CLTX-IgG4(hinge)-CD28tm-CD28-zeta excluding signal

<400>  44

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
    50                  55                  60                  


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
65                  70                  75                  80  


Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
                85                  90                  95      


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
            100                 105                 110         


Ala Ala Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala 
        115                 120                 125             


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    130                 135                 140                 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
145                 150                 155                 160 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                165                 170                 175     


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            180                 185                 190         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        195                 200                 205             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    210                 215                 220                 


His Met Gln Ala Leu Pro Pro Arg 
225                 230         


<210>  45
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-CD28-zeta excluding signal

<400>  45

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Met Phe 
        35                  40                  45              


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
    50                  55                  60                  


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
65                  70                  75                  80  


Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
                85                  90                  95      


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
            100                 105                 110         


Tyr Arg Ser Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
        115                 120                 125             


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
    130                 135                 140                 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
145                 150                 155                 160 


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
                165                 170                 175     


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
            180                 185                 190         


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
        195                 200                 205             


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
    210                 215                 220                 


Gln Ala Leu Pro Pro Arg 
225                 230 


<210>  46
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD28tm-CD28-4-1BBzeta excluding signal

<400>  46

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    50                  55                  60                  


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
65                  70                  75                  80  


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
                85                  90                  95      


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            100                 105                 110         


Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        115                 120                 125             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    130                 135                 140                 


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
145                 150                 155                 160 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met 
                165                 170                 175     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            180                 185                 190         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        195                 200                 205             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    210                 215                 220                 


Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val 
225                 230                 235                 240 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                245                 250                 255     


Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val 
            260                 265                 270         


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
        275                 280                 285             


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp 
    290                 295                 300                 


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
305                 310                 315                 320 


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly 
                325                 330                 335     


Gly Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            340                 345                 350         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        355                 360                 365             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val 
    370                 375                 380                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
385                 390                 395                 400 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                405                 410                 415     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            420                 425                 430         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        435                 440                 445             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    450                 455                 460                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 


<210>  47
<211>  394
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD28tm-CD28-4-1BBzeta excluding signal

<400>  47

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro 
    50                  55                  60                  


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
65                  70                  75                  80  


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                85                  90                  95      


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            100                 105                 110         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
        115                 120                 125             


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
    130                 135                 140                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
145                 150                 155                 160 


Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
                165                 170                 175     


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
            180                 185                 190         


Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met 
        195                 200                 205             


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
    210                 215                 220                 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Gly Gly Gly Lys Arg Gly 
225                 230                 235                 240 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
                245                 250                 255     


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
            260                 265                 270         


Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg 
        275                 280                 285             


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    290                 295                 300                 


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
305                 310                 315                 320 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                325                 330                 335     


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            340                 345                 350         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        355                 360                 365             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    370                 375                 380                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
385                 390                 


<210>  48
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h-CD28tm-CD28-4-1BB-zeta excluding signal

<400>  48

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        35                  40                  45              


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    50                  55                  60                  


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
65                  70                  75                  80  


Asp Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
                85                  90                  95      


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys 
            100                 105                 110         


Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
        115                 120                 125             


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
    130                 135                 140                 


Phe Ala Ala Tyr Arg Ser Gly Gly Gly Lys Arg Gly Arg Lys Lys Leu 
145                 150                 155                 160 


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
                165                 170                 175     


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
            180                 185                 190         


Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
        195                 200                 205             


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
    210                 215                 220                 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
225                 230                 235                 240 


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
                245                 250                 255     


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
            260                 265                 270         


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
        275                 280                 285             


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
    290                 295                 300                 


Gln Ala Leu Pro Pro Arg 
305                 310 


<210>  49
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric  LTX-IgG4(hinge)-CD28tm-CD28-4-1BBzeta   excluding 
       signal

<400>  49

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
    50                  55                  60                  


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg 
65                  70                  75                  80  


Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
                85                  90                  95      


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
            100                 105                 110         


Ala Ala Tyr Arg Ser Gly Gly Gly Lys Arg Gly Arg Lys Lys Leu Leu 
        115                 120                 125             


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
    130                 135                 140                 


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
145                 150                 155                 160 


Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
                165                 170                 175     


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
            180                 185                 190         


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
        195                 200                 205             


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
    210                 215                 220                 


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
225                 230                 235                 240 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
                245                 250                 255     


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            260                 265                 270         


Ala Leu Pro Pro Arg 
        275         


<210>  50
<211>  275
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-CD28-4-1BB-zeta excluding signal

<400>  50

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Met Phe 
        35                  40                  45              


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
    50                  55                  60                  


Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg 
65                  70                  75                  80  


Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro 
                85                  90                  95      


Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala 
            100                 105                 110         


Tyr Arg Ser Gly Gly Gly Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 
        115                 120                 125             


Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 
    130                 135                 140                 


Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
145                 150                 155                 160 


Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
                165                 170                 175     


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
            180                 185                 190         


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
        195                 200                 205             


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    210                 215                 220                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
225                 230                 235                 240 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                245                 250                 255     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            260                 265                 270         


Pro Pro Arg 
        275 


<210>  51
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(EQ)-CD4tm-4-1BB-zeta excluding signal

<400>  51

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
    50                  55                  60                  


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
65                  70                  75                  80  


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
                85                  90                  95      


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
            100                 105                 110         


Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
        115                 120                 125             


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
    130                 135                 140                 


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
145                 150                 155                 160 


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met 
                165                 170                 175     


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
            180                 185                 190         


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
        195                 200                 205             


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
    210                 215                 220                 


Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val 
225                 230                 235                 240 


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
                245                 250                 255     


Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Ala Leu Ile Val Leu Gly 
            260                 265                 270         


Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Lys 
        275                 280                 285             


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
    290                 295                 300                 


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
305                 310                 315                 320 


Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe 
                325                 330                 335     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
            340                 345                 350         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        355                 360                 365             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
    370                 375                 380                 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
385                 390                 395                 400 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                405                 410                 415     


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            420                 425                 430         


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        435                 440                 


<210>  52
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(HL-CH3)-CD4tm-4-1BB-zeta excluding signal

<400>  52

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Gly Gln Pro Arg Glu Pro 
    50                  55                  60                  


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
65                  70                  75                  80  


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
                85                  90                  95      


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
            100                 105                 110         


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
        115                 120                 125             


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
    130                 135                 140                 


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
145                 150                 155                 160 


Leu Ser Leu Gly Lys Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly 
                165                 170                 175     


Leu Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Lys Arg Gly Arg Lys 
            180                 185                 190         


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
        195                 200                 205             


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
    210                 215                 220                 


Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala 
225                 230                 235                 240 


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
                245                 250                 255     


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
            260                 265                 270         


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
        275                 280                 285             


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
    290                 295                 300                 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
305                 310                 315                 320 


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
                325                 330                 335     


His Met Gln Ala Leu Pro Pro Arg 
            340                 


<210>  53
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-CD8h- CD28tm-4-1BB-zeta signal

<400>  53

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        35                  40                  45              


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    50                  55                  60                  


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
65                  70                  75                  80  


Asp Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr 
                85                  90                  95      


Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly 
            100                 105                 110         


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
        115                 120                 125             


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
    130                 135                 140                 


Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg 
145                 150                 155                 160 


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
                165                 170                 175     


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            180                 185                 190         


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
        195                 200                 205             


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
    210                 215                 220                 


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
225                 230                 235                 240 


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
                245                 250                 255     


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            260                 265     


<210>  54
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-IgG4(hinge)-CD28tm-4-1BB-zeta excluding signal

<400>  54

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
        35                  40                  45              


Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
    50                  55                  60                  


Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg 
65                  70                  75                  80  


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
                85                  90                  95      


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
            100                 105                 110         


Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser 
        115                 120                 125             


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    130                 135                 140                 


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
145                 150                 155                 160 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                165                 170                 175     


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            180                 185                 190         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        195                 200                 205             


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    210                 215                 220                 


Leu His Met Gln Ala Leu Pro Pro Arg 
225                 230             


<210>  55
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CLTX-L-CD28tm-4-1BB-zeta excluding signal

<400>  55

Met Cys Met Pro Cys Phe Thr Thr Asp His Gln Met Ala Arg Lys Cys 
1               5                   10                  15      


Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly Lys Cys Tyr Gly Pro Gln 
            20                  25                  30          


Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly Gly Gly Ser Gly Met Phe 
        35                  40                  45              


Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu 
    50                  55                  60                  


Val Thr Val Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys 
65                  70                  75                  80  


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
                85                  90                  95      


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
            100                 105                 110         


Gly Cys Glu Leu Gly Gly Gly Arg Val Lys Phe Ser Arg Ser Ala Asp 
        115                 120                 125             


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
    130                 135                 140                 


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
145                 150                 155                 160 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
                165                 170                 175     


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
            180                 185                 190         


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
        195                 200                 205             


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
    210                 215                 220                 


Met Gln Ala Leu Pro Pro Arg 
225                 230     


<210>  56
<211>  29
<212>  PRT
<213>  Leiurus quinquestriatus hebraeus

<400>  56

Val Ser Cys Glu Asp Cys Pro Asp His Cys Ser Thr Gln Lys Ala Arg 
1               5                   10                  15      


Ala Lys Cys Asp Asn Asp Lys Cys Val Cys Glu Pro Ile 
            20                  25                  


<210>  57
<211>  34
<212>  PRT
<213>  Leiurus quinquestriatus hebraeus

<400>  57

Cys Gly Pro Cys Phe Thr Thr Asp His Gln Met Glu Gln Lys Cys Ala 
1               5                   10                  15      


Glu Cys Cys Gly Gly Ile Gly Lys Cys Tyr Gly Pro Gln Cys Leu Cys 
            20                  25                  30          


Asn Arg 
        


<210>  58
<211>  34
<212>  PRT
<213>  Androctonus australis

<400>  58

Met Cys Ile Pro Cys Phe Thr Thr Asn Pro Asn Met Ala Ala Lys Cys 
1               5                   10                  15      


Asn Ala Cys Cys Gly Ser Arg Arg Gly Ser Cys Arg Gly Pro Gln Cys 
            20                  25                  30          


Ile Cys 
        


<210>  59
<211>  35
<212>  PRT
<213>  Buthus martensii

<400>  59

Cys Gly Pro Cys Phe Thr Thr Asp Ala Asn Met Ala Arg Lys Cys Arg 
1               5                   10                  15      


Glu Cys Cys Gly Gly Ile Gly Lys Cys Phe Gly Pro Gln Cys Leu Cys 
            20                  25                  30          


Asn Arg Ile 
        35  


<210>  60
<211>  600
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chimeric CTLX-L-CD28tm-41BB-zeta-T2A-CD19

<400>  60

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Cys Met Pro Cys Phe Thr Thr Asp His 
            20                  25                  30          


Gln Met Ala Arg Lys Cys Asp Asp Cys Cys Gly Gly Lys Gly Arg Gly 
        35                  40                  45              


Lys Cys Tyr Gly Pro Gln Cys Leu Cys Arg Gly Gly Gly Ser Ser Gly 
    50                  55                  60                  


Gly Gly Ser Gly Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
65                  70                  75                  80  


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
                85                  90                  95      


Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
            100                 105                 110         


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
        115                 120                 125             


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Gly Gly Gly Arg Val Lys 
    130                 135                 140                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
145                 150                 155                 160 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                165                 170                 175     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            180                 185                 190         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        195                 200                 205             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    210                 215                 220                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
225                 230                 235                 240 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly 
                245                 250                 255     


Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
            260                 265                 270         


Asn Pro Gly Pro Arg Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu 
        275                 280                 285             


Phe Leu Thr Pro Met Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys 
    290                 295                 300                 


Val Glu Glu Gly Asp Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser 
305                 310                 315                 320 


Asp Gly Pro Thr Gln Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys 
                325                 330                 335     


Pro Phe Leu Lys Leu Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met 
            340                 345                 350         


Arg Pro Leu Ala Ile Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met 
        355                 360                 365             


Gly Gly Phe Tyr Leu Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp 
    370                 375                 380                 


Gln Pro Gly Trp Thr Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg 
385                 390                 395                 400 


Trp Asn Val Ser Asp Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg 
                405                 410                 415     


Ser Ser Glu Gly Pro Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys 
            420                 425                 430         


Leu Tyr Val Trp Ala Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro 
        435                 440                 445             


Pro Cys Val Pro Pro Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp 
    450                 455                 460                 


Leu Thr Met Ala Pro Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro 
465                 470                 475                 480 


Pro Asp Ser Val Ser Arg Gly Pro Leu Ser Trp Thr His Val His Pro 
                485                 490                 495     


Lys Gly Pro Lys Ser Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro 
            500                 505                 510         


Ala Arg Asp Met Trp Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala 
        515                 520                 525             


Thr Ala Gln Asp Ala Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr 
    530                 535                 540                 


Met Ser Phe His Leu Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp 
545                 550                 555                 560 


Leu Leu Arg Thr Gly Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr 
                565                 570                 575     


Leu Ile Phe Cys Leu Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg 
            580                 585                 590         


Ala Leu Val Leu Arg Arg Lys Arg 
        595                 600 


