
                                SEQUENCE LISTING

<110> Qiagen Sciences LLC
      Wang, Yexun
      Peng, Quan

<120> METHODS AND KITS FOR HIGHLY MULTIPLEX
  SINGLE PRIMER EXTENSION
  

<130> 830109.409WO

<140> PCT                 
<141> 2016-10-14  

<150> US 62/242,766       
<151> 2015-10-16  

<160> 6

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 853
<212> PRT
<213> Escherichia coli

<400> 1
Met Ser Ala Ile Glu Asn Phe Asp Ala His Thr Pro Met Met Gln Gln
 1               5                  10                  15
Tyr Leu Arg Leu Lys Ala Gln His Pro Glu Ile Leu Leu Phe Tyr Arg
            20                  25                  30
Met Gly Asp Phe Tyr Glu Leu Phe Tyr Asp Asp Ala Lys Arg Ala Ser
        35                  40                  45
Gln Leu Leu Asp Ile Ser Leu Thr Lys Arg Gly Ala Ser Ala Gly Glu
    50                  55                  60
Pro Ile Pro Met Ala Gly Ile Pro Tyr His Ala Val Glu Asn Tyr Leu
65                  70                  75                  80
Ala Lys Leu Val Asn Gln Gly Glu Ser Val Ala Ile Cys Glu Gln Ile
                85                  90                  95
Gly Asp Pro Ala Thr Ser Lys Gly Pro Val Glu Arg Lys Val Val Arg
            100                 105                 110
Ile Val Thr Pro Gly Thr Ile Ser Asp Glu Ala Leu Leu Gln Glu Arg
        115                 120                 125
Gln Asp Asn Leu Leu Ala Ala Ile Trp Gln Asp Ser Lys Gly Phe Gly
    130                 135                 140
Tyr Ala Thr Leu Asp Ile Ser Ser Gly Arg Phe Arg Leu Ser Glu Pro
145                 150                 155                 160
Ala Asp Arg Glu Thr Met Ala Ala Glu Leu Gln Arg Thr Asn Pro Ala
                165                 170                 175
Glu Leu Leu Tyr Ala Glu Asp Phe Ala Glu Met Ser Leu Ile Glu Gly
            180                 185                 190
Arg Arg Gly Leu Arg Arg Arg Pro Leu Trp Glu Phe Glu Ile Asp Thr
        195                 200                 205
Ala Arg Gln Gln Leu Asn Leu Gln Phe Gly Thr Arg Asp Leu Val Gly
    210                 215                 220
Phe Gly Val Glu Asn Ala Pro Arg Gly Leu Cys Ala Ala Gly Cys Leu
225                 230                 235                 240
Leu Gln Tyr Ala Lys Asp Thr Gln Arg Thr Thr Leu Pro His Ile Arg
                245                 250                 255
Ser Ile Thr Met Glu Arg Glu Gln Asp Ser Ile Ile Met Asp Ala Ala
            260                 265                 270
Thr Arg Arg Asn Leu Glu Ile Thr Gln Asn Leu Ala Gly Gly Ala Glu
        275                 280                 285
Asn Thr Leu Ala Ser Val Leu Asp Cys Thr Val Thr Pro Met Gly Ser
    290                 295                 300
Arg Met Leu Lys Arg Trp Leu His Met Pro Val Arg Asp Thr Arg Val
305                 310                 315                 320
Leu Leu Glu Arg Gln Gln Thr Ile Gly Ala Leu Gln Asp Phe Thr Ala
                325                 330                 335
Gly Leu Gln Pro Val Leu Arg Gln Val Gly Asp Leu Glu Arg Ile Leu
            340                 345                 350
Ala Arg Leu Ala Leu Arg Thr Ala Arg Pro Arg Asp Leu Ala Arg Met
        355                 360                 365
Arg His Ala Phe Gln Gln Leu Pro Glu Leu Arg Ala Gln Leu Glu Thr
    370                 375                 380
Val Asp Ser Ala Pro Val Gln Ala Leu Arg Glu Lys Met Gly Glu Phe
385                 390                 395                 400
Ala Glu Leu Arg Asp Leu Leu Glu Arg Ala Ile Ile Asp Thr Pro Pro
                405                 410                 415
Val Leu Val Arg Asp Gly Gly Val Ile Ala Ser Gly Tyr Asn Glu Glu
            420                 425                 430
Leu Asp Glu Trp Arg Ala Leu Ala Asp Gly Ala Thr Asp Tyr Leu Glu
        435                 440                 445
Arg Leu Glu Val Arg Glu Arg Glu Arg Thr Gly Leu Asp Thr Leu Lys
    450                 455                 460
Val Gly Phe Asn Ala Val His Gly Tyr Tyr Ile Gln Ile Ser Arg Gly
465                 470                 475                 480
Gln Ser His Leu Ala Pro Ile Asn Tyr Met Arg Arg Gln Thr Leu Lys
                485                 490                 495
Asn Ala Glu Arg Tyr Ile Ile Pro Glu Leu Lys Glu Tyr Glu Asp Lys
            500                 505                 510
Val Leu Thr Ser Lys Gly Lys Ala Leu Ala Leu Glu Lys Gln Leu Tyr
        515                 520                 525
Glu Glu Leu Phe Asp Leu Leu Leu Pro His Leu Glu Ala Leu Gln Gln
    530                 535                 540
Ser Ala Ser Ala Leu Ala Glu Leu Asp Val Leu Val Asn Leu Ala Glu
545                 550                 555                 560
Arg Ala Tyr Thr Leu Asn Tyr Thr Cys Pro Thr Phe Ile Asp Lys Pro
                565                 570                 575
Gly Ile Arg Ile Thr Glu Gly Arg His Pro Val Val Glu Gln Val Leu
            580                 585                 590
Asn Glu Pro Phe Ile Ala Asn Pro Leu Asn Leu Ser Pro Gln Arg Arg
        595                 600                 605
Met Leu Ile Ile Thr Gly Pro Asn Met Gly Gly Lys Ser Thr Tyr Met
    610                 615                 620
Arg Gln Thr Ala Leu Ile Ala Leu Met Ala Tyr Ile Gly Ser Tyr Val
625                 630                 635                 640
Pro Ala Gln Lys Val Glu Ile Gly Pro Ile Asp Arg Ile Phe Thr Arg
                645                 650                 655
Val Gly Ala Ala Asp Asp Leu Ala Ser Gly Arg Ser Thr Phe Met Val
            660                 665                 670
Glu Met Thr Glu Thr Ala Asn Ile Leu His Asn Ala Thr Glu Tyr Ser
        675                 680                 685
Leu Val Leu Met Asp Glu Ile Gly Arg Gly Thr Ser Thr Tyr Asp Gly
    690                 695                 700
Leu Ser Leu Ala Trp Ala Cys Ala Glu Asn Leu Ala Asn Lys Ile Lys
705                 710                 715                 720
Ala Leu Thr Leu Phe Ala Thr His Tyr Phe Glu Leu Thr Gln Leu Pro
                725                 730                 735
Glu Lys Met Glu Gly Val Ala Asn Val His Leu Asp Ala Leu Glu His
            740                 745                 750
Gly Asp Thr Ile Ala Phe Met His Ser Val Gln Asp Gly Ala Ala Ser
        755                 760                 765
Lys Ser Tyr Gly Leu Ala Val Ala Ala Leu Ala Gly Val Pro Lys Glu
    770                 775                 780
Val Ile Lys Arg Ala Arg Gln Lys Leu Arg Glu Leu Glu Ser Ile Ser
785                 790                 795                 800
Pro Asn Ala Ala Ala Thr Gln Val Asp Gly Thr Gln Met Ser Leu Leu
                805                 810                 815
Ser Val Pro Glu Glu Thr Ser Pro Ala Val Glu Ala Leu Glu Asn Leu
            820                 825                 830
Asp Pro Asp Ser Leu Thr Pro Arg Gln Ala Leu Glu Trp Ile Tyr Arg
        835                 840                 845
Leu Lys Ser Leu Val
    850

<210> 2
<211> 811
<212> PRT
<213> Thermus aquaticus

<400> 2
Met Glu Gly Met Leu Lys Gly Glu Gly Pro Gly Pro Leu Pro Pro Leu
 1               5                  10                  15
Leu Gln Gln Tyr Val Glu Leu Arg Asp Gln Tyr Pro Asp Tyr Leu Leu
            20                  25                  30
Leu Phe Gln Val Gly Asp Phe Tyr Glu Cys Phe Gly Glu Asp Ala Glu
        35                  40                  45
Arg Leu Ala Arg Ala Leu Gly Leu Val Leu Thr His Lys Thr Ser Lys
    50                  55                  60
Asp Phe Thr Thr Pro Met Ala Gly Ile Pro Leu Arg Ala Phe Glu Ala
65                  70                  75                  80
Tyr Ala Glu Arg Leu Leu Lys Met Gly Phe Arg Leu Ala Val Ala Asp
                85                  90                  95
Gln Val Glu Pro Ala Glu Glu Ala Glu Gly Leu Val Arg Arg Glu Val
            100                 105                 110
Thr Gln Leu Leu Thr Pro Gly Thr Leu Leu Gln Glu Ser Leu Leu Pro
        115                 120                 125
Arg Glu Ala Asn Tyr Leu Ala Ala Ile Ala Thr Gly Asp Gly Trp Gly
    130                 135                 140
Leu Ala Phe Leu Asp Val Ser Thr Gly Glu Phe Lys Gly Thr Val Leu
145                 150                 155                 160
Lys Ser Lys Ser Ala Leu Tyr Asp Glu Leu Phe Arg His Arg Pro Ala
                165                 170                 175
Glu Val Leu Leu Ala Pro Glu Leu Leu Glu Asn Gly Ala Phe Leu Asp
            180                 185                 190
Glu Phe Arg Lys Arg Phe Pro Val Met Leu Ser Glu Ala Pro Phe Glu
        195                 200                 205
Pro Glu Gly Glu Gly Pro Leu Ala Leu Arg Arg Ala Arg Gly Ala Leu
    210                 215                 220
Leu Ala Tyr Ala Gln Arg Thr Gln Gly Gly Ala Leu Ser Leu Gln Pro
225                 230                 235                 240
Phe Arg Phe Tyr Asp Pro Gly Ala Phe Met Arg Leu Pro Glu Ala Thr
                245                 250                 255
Leu Arg Ala Leu Glu Val Phe Glu Pro Leu Arg Gly Gln Asp Thr Leu
            260                 265                 270
Phe Ser Val Leu Asp Glu Thr Arg Thr Ala Pro Gly Arg Arg Leu Leu
        275                 280                 285
Gln Ser Trp Leu Arg His Pro Leu Leu Asp Arg Gly Pro Leu Glu Ala
    290                 295                 300
Arg Leu Asp Arg Val Glu Gly Phe Val Arg Glu Gly Ala Leu Arg Glu
305                 310                 315                 320
Gly Val Arg Arg Leu Leu Tyr Arg Leu Ala Asp Leu Glu Arg Leu Ala
                325                 330                 335
Thr Arg Leu Glu Leu Gly Arg Ala Ser Pro Lys Asp Leu Gly Ala Leu
            340                 345                 350
Arg Arg Ser Leu Gln Ile Leu Pro Glu Leu Arg Ala Leu Leu Gly Glu
        355                 360                 365
Glu Val Gly Leu Pro Asp Leu Ser Pro Leu Lys Glu Glu Leu Glu Ala
    370                 375                 380
Ala Leu Val Glu Asp Pro Pro Leu Lys Val Ser Glu Gly Gly Leu Ile
385                 390                 395                 400
Arg Glu Gly Tyr Asp Pro Asp Leu Asp Ala Leu Arg Ala Ala His Arg
                405                 410                 415
Glu Gly Val Ala Tyr Phe Leu Glu Leu Glu Glu Arg Glu Arg Glu Arg
            420                 425                 430
Thr Gly Ile Pro Thr Leu Lys Val Gly Tyr Asn Ala Val Phe Gly Tyr
        435                 440                 445
Tyr Leu Glu Val Thr Arg Pro Tyr Tyr Glu Arg Val Pro Lys Glu Tyr
    450                 455                 460
Arg Pro Val Gln Thr Leu Lys Asp Arg Gln Arg Tyr Thr Leu Pro Glu
465                 470                 475                 480
Met Lys Glu Lys Glu Arg Glu Val Tyr Arg Leu Glu Ala Leu Ile Arg
                485                 490                 495
Arg Arg Glu Glu Glu Val Phe Leu Glu Val Arg Glu Arg Ala Lys Arg
            500                 505                 510
Gln Ala Glu Ala Leu Arg Glu Ala Ala Arg Ile Leu Ala Glu Leu Asp
        515                 520                 525
Val Tyr Ala Ala Leu Ala Glu Val Ala Val Arg Tyr Gly Tyr Val Arg
    530                 535                 540
Pro Arg Phe Gly Asp Arg Leu Gln Ile Arg Ala Gly Arg His Pro Val
545                 550                 555                 560
Val Glu Arg Arg Thr Glu Phe Val Pro Asn Asp Leu Glu Met Ala His
                565                 570                 575
Glu Leu Val Leu Ile Thr Gly Pro Asn Met Ala Gly Lys Ser Thr Phe
            580                 585                 590
Leu Arg Gln Thr Ala Leu Ile Ala Leu Leu Ala Gln Val Gly Ser Phe
        595                 600                 605
Val Pro Ala Glu Glu Ala His Leu Pro Leu Phe Asp Gly Ile Tyr Thr
    610                 615                 620
Arg Ile Gly Ala Ser Asp Asp Leu Ala Gly Gly Lys Ser Thr Phe Met
625                 630                 635                 640
Val Glu Met Glu Glu Val Ala Leu Ile Leu Lys Glu Ala Thr Glu Asn
                645                 650                 655
Ser Leu Val Leu Leu Asp Glu Val Gly Arg Gly Thr Ser Ser Leu Asp
            660                 665                 670
Gly Val Ala Ile Ala Thr Ala Val Ala Glu Ala Leu His Glu Arg Arg
        675                 680                 685
Ala Tyr Thr Leu Phe Ala Thr His Tyr Phe Glu Leu Thr Ala Leu Gly
    690                 695                 700
Leu Pro Arg Leu Lys Asn Leu His Val Ala Ala Arg Glu Glu Ala Gly
705                 710                 715                 720
Gly Leu Val Phe Tyr His Gln Val Leu Pro Gly Pro Ala Ser Lys Ser
                725                 730                 735
Tyr Gly Val Glu Val Ala Ala Met Ala Gly Leu Pro Lys Glu Val Val
            740                 745                 750
Ala Arg Ala Arg Ala Leu Leu Gln Ala Met Ala Ala Arg Arg Glu Gly
        755                 760                 765
Ala Leu Asp Ala Val Leu Glu Arg Leu Leu Ala Leu Asp Pro Asp Arg
    770                 775                 780
Leu Thr Pro Leu Glu Ala Leu Arg Leu Leu Gln Glu Leu Lys Ala Leu
785                 790                 795                 800
Ala Leu Gly Ala Pro Leu Asp Thr Met Lys Gly
                805                 810

<210> 3
<211> 819
<212> PRT
<213> Thermus thermophilus

<400> 3
Met Gly Gly Tyr Gly Gly Val Lys Met Glu Gly Met Leu Lys Gly Glu
 1               5                  10                  15
Gly Pro Gly Pro Leu Pro Pro Leu Leu Gln Gln Tyr Val Glu Leu Arg
            20                  25                  30
Asp Arg Tyr Pro Asp Tyr Leu Leu Leu Phe Gln Val Gly Asp Phe Tyr
        35                  40                  45
Glu Cys Phe Gly Glu Asp Ala Glu Arg Leu Ala Arg Ala Leu Gly Leu
    50                  55                  60
Val Leu Thr His Lys Thr Ser Lys Asp Phe Thr Thr Pro Met Ala Gly
65                  70                  75                  80
Ile Pro Ile Arg Ala Phe Asp Ala Tyr Ala Glu Arg Leu Leu Lys Met
                85                  90                  95
Gly Phe Arg Leu Ala Val Ala Asp Gln Val Glu Pro Ala Glu Glu Ala
            100                 105                 110
Glu Gly Leu Val Arg Arg Glu Val Thr Gln Leu Leu Thr Pro Gly Thr
        115                 120                 125
Leu Thr Gln Glu Ala Leu Leu Pro Arg Glu Ala Asn Tyr Leu Ala Ala
    130                 135                 140
Ile Ala Thr Gly Asp Gly Trp Gly Leu Ala Phe Leu Asp Val Ser Thr
145                 150                 155                 160
Gly Glu Phe Lys Gly Thr Leu Leu Lys Ser Lys Ser Ala Leu Tyr Asp
                165                 170                 175
Glu Leu Phe Arg His Arg Pro Ala Glu Val Leu Leu Ala Pro Glu Leu
            180                 185                 190
Arg Glu Asn Glu Ala Phe Val Ala Glu Phe Arg Lys Arg Phe Pro Val
        195                 200                 205
Met Leu Ser Glu Ala Pro Phe Glu Pro Gln Gly Glu Gly Pro Leu Ala
    210                 215                 220
Leu Arg Arg Ala Gln Gly Ala Leu Leu Ala Tyr Ala Arg Ala Thr Gln
225                 230                 235                 240
Gly Gly Ala Leu Ser Val Arg Pro Phe Arg Leu Tyr Asp Pro Gly Ala
                245                 250                 255
Phe Val Arg Leu Pro Glu Ala Ser Leu Lys Ala Leu Glu Val Phe Glu
            260                 265                 270
Pro Leu Arg Gly Gln Asp Thr Leu Phe Gly Val Leu Asp Glu Thr Arg
        275                 280                 285
Thr Ala Pro Gly Arg Arg Leu Leu Gln Ala Trp Leu Arg His Pro Leu
    290                 295                 300
Leu Glu Arg Gly Pro Leu Glu Ala Arg Leu Asp Arg Val Glu Arg Phe
305                 310                 315                 320
Val Arg Glu Gly Ala Leu Arg Glu Gly Val Arg Arg Leu Leu Phe Arg
                325                 330                 335
Leu Ala Asp Leu Glu Arg Leu Ala Thr Arg Leu Glu Leu Ser Arg Ala
            340                 345                 350
Ser Pro Arg Asp Leu Ala Ala Leu Arg Arg Ser Leu Glu Ile Leu Pro
        355                 360                 365
Glu Leu Lys Gly Leu Leu Gly Glu Glu Val Gly Leu Pro Asp Leu Ser
    370                 375                 380
Gly Leu Leu Glu Glu Leu Arg Ala Ala Leu Val Glu Asp Pro Pro Leu
385                 390                 395                 400
Lys Val Ser Glu Gly Gly Leu Ile Arg Glu Gly Tyr Asp Pro Asp Leu
                405                 410                 415
Asp Ala Leu Arg Arg Ala His Ala Glu Gly Val Ala Tyr Phe Leu Asp
            420                 425                 430
Leu Glu Ala Arg Glu Lys Glu Arg Thr Gly Ile Pro Thr Leu Lys Val
        435                 440                 445
Gly Tyr Asn Ala Val Phe Gly Tyr Tyr Leu Glu Val Thr Arg Pro Tyr
    450                 455                 460
Tyr Glu Lys Val Pro Gln Glu Tyr Arg Pro Val Gln Thr Leu Lys Asp
465                 470                 475                 480
Arg Gln Arg Tyr Thr Leu Pro Glu Met Lys Glu Arg Glu Arg Glu Leu
                485                 490                 495
Tyr Arg Leu Glu Ala Leu Ile Lys Arg Arg Glu Glu Glu Val Phe Leu
            500                 505                 510
Ala Leu Arg Glu Arg Ala Arg Lys Glu Ala Glu Ala Leu Arg Glu Ala
        515                 520                 525
Ala Arg Ile Leu Ala Glu Leu Asp Val Tyr Ala Ala Leu Ala Glu Val
    530                 535                 540
Ala Val Arg His Gly Tyr Thr Arg Pro Arg Phe Gly Glu Arg Leu Arg
545                 550                 555                 560
Ile Arg Ala Gly Arg His Pro Val Val Glu Arg Arg Thr Ala Phe Val
                565                 570                 575
Pro Asn Asp Leu Glu Met Ala His Glu Leu Val Leu Val Thr Gly Pro
            580                 585                 590
Asn Met Ala Gly Lys Ser Thr Phe Leu Arg Gln Thr Ala Leu Ile Ala
        595                 600                 605
Leu Leu Ala Gln Ile Gly Ser Phe Val Pro Ala Glu Glu Ala Glu Leu
    610                 615                 620
Pro Leu Phe Asp Gly Ile Tyr Thr Arg Ile Gly Ala Ser Asp Asp Leu
625                 630                 635                 640
Ala Gly Gly Lys Ser Thr Phe Met Val Glu Met Glu Glu Val Ala Leu
                645                 650                 655
Val Leu Lys Glu Ala Thr Glu Arg Ser Leu Val Leu Leu Asp Glu Val
            660                 665                 670
Gly Arg Gly Thr Ser Ser Leu Asp Gly Val Ala Ile Ala Thr Ala Leu
        675                 680                 685
Ala Glu Ala Leu His Glu Arg Arg Cys Tyr Thr Leu Phe Ala Thr His
    690                 695                 700
Tyr Phe Glu Leu Thr Ala Leu Ala Leu Pro Arg Leu Lys Asn Leu His
705                 710                 715                 720
Val Ala Ala Lys Glu Glu Glu Gly Gly Leu Val Phe Tyr His Gln Val
                725                 730                 735
Leu Pro Gly Pro Ala Ser Lys Ser Tyr Gly Val Glu Val Ala Glu Met
            740                 745                 750
Ala Gly Leu Pro Lys Glu Val Val Glu Arg Ala Arg Ala Leu Leu Ser
        755                 760                 765
Ala Met Ala Ala Arg Arg Glu Gly Ala Leu Glu Glu Val Leu Glu Arg
    770                 775                 780
Leu Leu Ala Leu Asp Pro Asp Arg Leu Thr Pro Leu Glu Ala Leu Arg
785                 790                 795                 800
Phe Leu His Glu Leu Lys Ala Leu Ala Leu Gly Leu Pro Leu Gly Ser
                805                 810                 815
Met Lys Gly


<210> 4
<211> 859
<212> PRT
<213> Aquifex aeolicus

<400> 4
Met Glu Lys Ser Glu Lys Glu Leu Thr Pro Met Leu Ser Gln Tyr His
 1               5                  10                  15
Tyr Phe Lys Asn Gln Tyr Pro Asp Cys Leu Leu Leu Phe Arg Leu Gly
            20                  25                  30
Asp Phe Tyr Glu Leu Phe Tyr Glu Asp Ala Tyr Ile Gly Ser Lys Glu
        35                  40                  45
Leu Gly Leu Val Leu Thr Ser Arg Pro Ala Gly Lys Gly Lys Glu Arg
    50                  55                  60
Ile Pro Met Cys Gly Val Pro Tyr His Ser Ala Asn Ser Tyr Ile Ala
65                  70                  75                  80
Lys Leu Val Asn Lys Gly Tyr Lys Val Ala Ile Cys Glu Gln Val Glu
                85                  90                  95
Asp Pro Ser Lys Ala Lys Gly Ile Val Lys Arg Glu Val Val Arg Val
            100                 105                 110
Ile Thr Pro Gly Thr Phe Phe Glu Arg Asp Thr Gly Gly Leu Ala Ser
        115                 120                 125
Leu Tyr Lys Lys Gly Asn His Tyr Tyr Val Gly Tyr Leu Asn Leu Ala
    130                 135                 140
Val Gly Glu Phe Leu Gly Ala Lys Val Lys Ile Glu Glu Leu Leu Asp
145                 150                 155                 160
Leu Leu Ser Lys Leu Asn Ile Lys Glu Ile Leu Val Lys Lys Gly Glu
                165                 170                 175
Lys Leu Pro Glu Glu Leu Glu Lys Val Leu Lys Val Tyr Val Ser Glu
            180                 185                 190
Leu Glu Glu Glu Phe Phe Glu Glu Gly Ser Glu Glu Ile Leu Lys Asp
        195                 200                 205
Phe Gly Val Leu Ser Leu Gln Ala Phe Gly Phe Glu Glu Asp Thr Tyr
    210                 215                 220
Ser Leu Pro Leu Gly Ala Val Tyr Lys Tyr Ala Lys Thr Thr Gln Lys
225                 230                 235                 240
Gly Tyr Thr Pro Leu Ile Pro Arg Pro Lys Pro Tyr Arg Asp Glu Gly
                245                 250                 255
Phe Val Arg Leu Asp Ile Lys Ala Ile Lys Gly Leu Glu Ile Leu Glu
            260                 265                 270
Ser Leu Glu Gly Arg Lys Asp Ile Ser Leu Phe Lys Val Ile Asp Arg
        275                 280                 285
Thr Leu Thr Gly Met Gly Arg Arg Arg Leu Lys Phe Arg Leu Leu Ser
    290                 295                 300
Pro Phe Arg Ser Arg Glu Lys Ile Glu Arg Ile Gln Glu Gly Val Gln
305                 310                 315                 320
Glu Leu Lys Glu Asn Arg Glu Ala Leu Leu Lys Ile Arg Gln Ile Leu
                325                 330                 335
Glu Gly Met Ala Asp Leu Glu Arg Leu Val Ser Lys Ile Ser Ser Asn
            340                 345                 350
Met Ala Thr Pro Arg Glu Leu Val Tyr Leu Lys Asn Ser Leu Lys Lys
        355                 360                 365
Val Glu Glu Leu Arg Leu Leu Leu Leu Glu Leu Lys Ala Pro Ile Phe
    370                 375                 380
Lys Glu Ile Leu Gln Asn Phe Glu Asp Thr Lys Lys Ile Ile Asn Asp
385                 390                 395                 400
Ile Glu Lys Thr Leu Val Glu Asp Pro Pro Leu His Val Lys Glu Gly
                405                 410                 415
Gly Leu Ile Arg Glu Gly Val Asn Ala Tyr Leu Asp Glu Leu Arg Phe
            420                 425                 430
Ile Arg Asp Asn Ala Glu Thr Tyr Leu Arg Glu Tyr Glu Lys Lys Leu
        435                 440                 445
Arg Gln Glu Thr Gly Ile Gln Ser Leu Lys Ile Gly Tyr Asn Lys Val
    450                 455                 460
Met Gly Tyr Tyr Ile Glu Val Thr Lys Pro Asn Leu Lys Tyr Val Pro
465                 470                 475                 480
Ser Tyr Phe Arg Arg Arg Gln Thr Leu Ser Asn Ser Glu Arg Phe Thr
                485                 490                 495
Thr Glu Glu Leu Gln Arg Leu Glu Glu Lys Ile Leu Ser Ala Gln Thr
            500                 505                 510
Arg Ile Asn Asp Leu Glu Tyr Glu Leu Tyr Lys Glu Leu Arg Glu Arg
        515                 520                 525
Val Val Lys Glu Leu Asp Lys Val Gly Asn Asn Ala Ser Ala Val Ala
    530                 535                 540
Glu Val Asp Phe Ile Gln Ser Leu Ala Gln Ile Ala Tyr Glu Lys Asp
545                 550                 555                 560
Trp Ala Lys Pro Gln Ile His Glu Gly Tyr Glu Leu Ile Ile Glu Glu
                565                 570                 575
Gly Arg His Pro Val Ile Glu Glu Phe Val Glu Asn Tyr Val Pro Asn
            580                 585                 590
Asp Thr Lys Leu Asp Arg Asp Ser Phe Ile His Val Ile Thr Gly Pro
        595                 600                 605
Asn Met Ala Gly Lys Ser Ser Tyr Ile Arg Gln Val Gly Val Leu Thr
    610                 615                 620
Leu Leu Ser His Ile Gly Ser Phe Ile Pro Ala Arg Arg Ala Lys Ile
625                 630                 635                 640
Pro Val Val Asp Ala Leu Phe Thr Arg Ile Gly Ser Gly Asp Val Leu
                645                 650                 655
Ala Leu Gly Val Ser Thr Phe Met Asn Glu Met Leu Glu Val Ser Asn
            660                 665                 670
Ile Leu Asn Asn Ala Thr Glu Lys Ser Leu Val Ile Leu Asp Glu Val
        675                 680                 685
Gly Arg Gly Thr Ser Thr Tyr Asp Gly Ile Ala Ile Ser Lys Ala Ile
    690                 695                 700
Val Lys Tyr Ile Ser Glu Lys Leu Lys Ala Lys Thr Leu Leu Ala Thr
705                 710                 715                 720
His Phe Leu Glu Ile Thr Glu Leu Glu Gly Lys Ile Glu Gly Val Lys
                725                 730                 735
Asn Tyr His Met Glu Val Glu Lys Thr Pro Glu Gly Ile Arg Phe Leu
            740                 745                 750
Tyr Ile Leu Lys Glu Gly Lys Ala Glu Gly Ser Phe Gly Ile Glu Val
        755                 760                 765
Ala Lys Leu Ala Gly Leu Pro Glu Glu Val Val Glu Glu Ala Arg Lys
    770                 775                 780
Ile Leu Arg Glu Leu Glu Glu Lys Glu Asn Lys Lys Glu Asp Ile Val
785                 790                 795                 800
Pro Leu Leu Glu Glu Thr Phe Lys Lys Ser Glu Glu Ala Gln Arg Leu
                805                 810                 815
Glu Glu Tyr Glu Glu Ile Ile Lys Lys Ile Glu Glu Ile Asp Ile Gly
            820                 825                 830
Asn Thr Thr Pro Leu Gln Ala Leu Leu Ile Leu Ala Glu Leu Lys Lys
        835                 840                 845
Lys Cys Ser Phe Ser Lys Lys Glu Ser Gly Ala
    850                 855

<210> 5
<211> 855
<212> PRT
<213> Aquifex pyrophilus

<400> 5
Met Gly Lys Glu Glu Lys Glu Leu Thr Pro Met Leu Ala Gln Tyr His
 1               5                  10                  15
Gln Phe Lys Ser Met Tyr Pro Asp Cys Leu Leu Leu Phe Arg Leu Gly
            20                  25                  30
Asp Phe Tyr Glu Leu Phe Tyr Glu Asp Ala Val Val Gly Ser Lys Glu
        35                  40                  45
Leu Gly Leu Val Leu Thr Ser Arg Pro Ala Gly Lys Gly Arg Glu Arg
    50                  55                  60
Ile Pro Met Cys Gly Val Pro Tyr His Ser Ala Asn Asn Tyr Ile Ala
65                  70                  75                  80
Lys Leu Val Asn Lys Gly Tyr Lys Val Ala Ile Cys Glu Gln Val Glu
                85                  90                  95
Asp Pro Ser Lys Ala Lys Gly Ile Val Lys Arg Asp Val Ile Arg Val
            100                 105                 110
Ile Thr Pro Gly Thr Phe Phe Glu Arg Glu Thr Gly Gly Leu Cys Ser
        115                 120                 125
Leu Tyr Arg Lys Gly Lys Ser Tyr Leu Val Ser Tyr Leu Asn Leu Ser
    130                 135                 140
Val Gly Glu Phe Ile Gly Ala Lys Val Lys Glu Glu Glu Leu Ile Asp
145                 150                 155                 160
Phe Leu Ser Lys Phe Asn Ile Arg Glu Val Leu Val Lys Lys Gly Glu
                165                 170                 175
Lys Leu Pro Glu Lys Leu Glu Lys Val Leu Lys Leu His Ile Thr Glu
            180                 185                 190
Leu Glu Glu Glu Phe Phe Glu Glu Gly Lys Glu Glu Leu Leu Lys Asp
        195                 200                 205
Tyr Gly Val Pro Ser Ile Lys Ala Phe Gly Phe Gln Asp Glu Asp Leu
    210                 215                 220
Ser Leu Ser Leu Gly Ala Val Tyr Arg Tyr Ala Lys Ala Thr Gln Lys
225                 230                 235                 240
Ser Phe Thr Pro Leu Ile Pro Lys Pro Lys Pro Tyr Val Asp Glu Gly
                245                 250                 255
Tyr Val Lys Leu Asp Leu Lys Ala Val Lys Gly Leu Glu Ile Thr Glu
            260                 265                 270
Ser Ile Glu Gly Arg Lys Asp Leu Ser Leu Phe Lys Val Val Asp Arg
        275                 280                 285
Thr Leu Thr Gly Met Gly Arg Arg Arg Leu Arg Phe Arg Leu Leu Asn
    290                 295                 300
Pro Phe Arg Ser Ile Glu Arg Ile Arg Lys Val Gln Glu Ala Val Glu
305                 310                 315                 320
Glu Leu Ile Asn Lys Arg Glu Val Leu Asn Glu Ile Arg Lys Thr Leu
                325                 330                 335
Glu Gly Met Ser Asp Leu Glu Arg Leu Val Ser Arg Ile Ser Ser Asn
            340                 345                 350
Met Ala Ser Pro Arg Glu Leu Ile His Leu Lys Asn Ser Leu Arg Lys
        355                 360                 365
Ala Glu Glu Leu Arg Lys Ile Leu Ser Leu Leu Asp Ser Glu Ile Phe
    370                 375                 380
Lys Glu Ile Glu Gly Ser Leu Leu Asn Leu Asn Lys Val Ala Asp Leu
385                 390                 395                 400
Ile Asp Lys Thr Leu Val Asp Asp Pro Pro Leu His Val Lys Glu Gly
                405                 410                 415
Gly Leu Ile Lys Pro Gly Val Asn Ala Tyr Leu Asp Glu Leu Arg Phe
            420                 425                 430
Ile Arg Glu Asn Ala Glu Lys Leu Leu Lys Glu Tyr Glu Lys Lys Leu
        435                 440                 445
Lys Lys Glu Thr Gly Ile Gln Ser Leu Lys Ile Gly Tyr Asn Lys Val
    450                 455                 460
Met Gly Tyr Tyr Ile Glu Val Thr Lys Ala Asn Val Lys Tyr Val Pro
465                 470                 475                 480
Glu His Phe Arg Arg Arg Gln Thr Leu Ser Asn Ala Glu Arg Tyr Thr
                485                 490                 495
Thr Glu Glu Leu Gln Arg Leu Glu Glu Lys Ile Leu Ser Ala Gln Thr
            500                 505                 510
Arg Ile Asn Glu Leu Glu Tyr Glu Leu Tyr Arg Glu Leu Arg Glu Glu
        515                 520                 525
Val Val Lys Glu Leu Asp Lys Val Gly Asn Asn Ala Thr Leu Ile Gly
    530                 535                 540
Glu Val Asp Tyr Ile Gln Ser Leu Ala Trp Leu Ala Leu Glu Lys Gly
545                 550                 555                 560
Trp Val Lys Pro Glu Val His Glu Gly Tyr Glu Leu Ile Ile Glu Glu
                565                 570                 575
Gly Lys His Pro Val Ile Glu Glu Phe Thr Lys Asn Tyr Val Pro Asn
            580                 585                 590
Asp Thr Lys Leu Thr Glu Glu Glu Phe Ile His Val Ile Thr Gly Pro
        595                 600                 605
Asn Met Ala Gly Lys Ser Ser Tyr Ile Arg Gln Val Gly Val Leu Thr
    610                 615                 620
Leu Leu Ala His Thr Gly Ser Phe Leu Pro Val Lys Ser Ala Arg Ile
625                 630                 635                 640
Pro Leu Val Asp Ala Ile Phe Thr Arg Ile Gly Ser Gly Asp Val Leu
                645                 650                 655
Ala Leu Gly Val Ser Thr Phe Met Asn Glu Met Leu Asp Val Ser Asn
            660                 665                 670
Ile Leu Asn Asn Ala Thr Lys Arg Ser Leu Ile Ile Leu Asp Glu Val
        675                 680                 685
Gly Arg Gly Thr Ser Thr Tyr Asp Gly Ile Ala Ile Ser Lys Ala Ile
    690                 695                 700
Val Lys Tyr Ile Ser Glu Lys Ile Gly Ala Lys Thr Leu Leu Ala Thr
705                 710                 715                 720
His Tyr Leu Glu Leu Thr Glu Leu Glu Arg Lys Val Lys Gly Val Lys
                725                 730                 735
Asn Tyr His Met Glu Val Glu Glu Thr Asp Glu Gly Ile Arg Phe Leu
            740                 745                 750
Tyr Ile Leu Lys Glu Gly Arg Ala Lys Gly Ser Phe Gly Ile Asp Val
        755                 760                 765
Ala Lys Leu Ala Gly Leu Pro Glu Glu Val Val Arg Glu Ala Lys Lys
    770                 775                 780
Ile Leu Lys Glu Leu Glu Gly Glu Lys Gly Lys Gln Glu Val Leu Pro
785                 790                 795                 800
Phe Leu Glu Glu Thr Tyr Lys Lys Ser Val Asp Glu Glu Lys Leu Asn
                805                 810                 815
Phe Tyr Glu Glu Ile Ile Lys Glu Ile Glu Glu Ile Asp Ile Gly Asn
            820                 825                 830
Thr Thr Pro Val Lys Ala Leu Leu Ile Leu Ala Glu Leu Lys Glu Arg
        835                 840                 845
Ile Lys Ser Phe Ile Lys Arg
    850                 855

<210> 6
<211> 793
<212> PRT
<213> Thermotoga maritima

<400> 6
Met Lys Val Thr Pro Leu Met Glu Gln Tyr Leu Arg Ile Lys Glu Gln
 1               5                  10                  15
Tyr Lys Asp Ser Ile Leu Leu Phe Arg Leu Gly Asp Phe Tyr Glu Ala
            20                  25                  30
Phe Phe Glu Asp Ala Lys Ile Val Ser Lys Val Leu Asn Ile Val Leu
        35                  40                  45
Thr Arg Arg Gln Asp Ala Pro Met Ala Gly Ile Pro Tyr His Ala Leu
    50                  55                  60
Asn Thr Tyr Leu Lys Lys Leu Val Glu Ala Gly Tyr Lys Val Ala Ile
65                  70                  75                  80
Cys Asp Gln Met Glu Glu Pro Ser Lys Ser Lys Lys Leu Ile Arg Arg
                85                  90                  95
Glu Val Thr Arg Val Val Thr Pro Gly Ser Ile Val Glu Asp Glu Phe
            100                 105                 110
Leu Ser Glu Thr Asn Asn Tyr Met Ala Val Val Ser Glu Glu Lys Gly
        115                 120                 125
Arg Tyr Cys Thr Val Phe Cys Asp Val Ser Thr Gly Glu Val Leu Val
    130                 135                 140
His Glu Ser Ser Asp Glu Gln Glu Thr Leu Asp Leu Leu Lys Asn Tyr
145                 150                 155                 160
Ser Ile Ser Gln Ile Ile Cys Pro Glu His Leu Lys Ser Ser Leu Lys
                165                 170                 175
Glu Arg Phe Pro Gly Val Tyr Thr Glu Thr Ile Ser Glu Trp Tyr Phe
            180                 185                 190
Ser Asp Leu Glu Glu Val Glu Lys Ala Tyr Asn Leu Lys Asp Ile His
        195                 200                 205
His Phe Glu Leu Ser Pro Leu Ala Leu Lys Ala Leu Ala Ala Leu Ile
    210                 215                 220
Lys Tyr Val Lys Tyr Thr Met Ile Gly Glu Asp Leu Asn Leu Lys Pro
225                 230                 235                 240
Pro Leu Leu Ile Ser Gln Arg Asp Tyr Met Ile Leu Asp Ser Ala Thr
                245                 250                 255
Val Glu Asn Leu Ser Trp Ile Pro Gly Asp Arg Gly Lys Asn Leu Phe
            260                 265                 270
Asp Val Leu Asn Asn Thr Glu Thr Pro Met Gly Ala Arg Leu Gly Lys
        275                 280                 285
Lys Trp Ile Leu His Pro Leu Val Asp Arg Lys Gln Ile Glu Glu Arg
    290                 295                 300
Leu Lys Ala Val Glu Arg Leu Val Asn Asp Arg Val Ser Leu Glu Glu
305                 310                 315                 320
Met Arg Asn Leu Leu Ser Asn Val Arg Asp Val Glu Arg Ile Val Ser
                325                 330                 335
Arg Val Glu Tyr Asn Arg Ser Val Pro Arg Asp Leu Val Ala Leu Arg
            340                 345                 350
Glu Thr Leu Glu Ile Ile Pro Lys Leu Asn Glu Val Leu Ser Thr Phe
        355                 360                 365
Gly Val Phe Lys Lys Leu Ala Phe Pro Glu Gly Leu Val Asp Leu Leu
    370                 375                 380
Arg Lys Ala Ile Glu Asp Asp Pro Val Gly Ser Pro Gly Glu Gly Lys
385                 390                 395                 400
Val Ile Lys Arg Gly Phe Ser Ser Glu Leu Asp Glu Tyr Arg Asp Leu
                405                 410                 415
Leu Glu His Ala Glu Glu Arg Leu Lys Glu Phe Glu Glu Lys Glu Arg
            420                 425                 430
Glu Arg Thr Gly Ile Gln Lys Leu Arg Val Gly Tyr Asn Gln Val Phe
        435                 440                 445
Gly Tyr Tyr Ile Glu Val Thr Lys Ala Asn Leu Asp Lys Ile Pro Asp
    450                 455                 460
Asp Tyr Glu Arg Lys Gln Thr Leu Val Asn Ser Glu Arg Phe Ile Thr
465                 470                 475                 480
Pro Glu Leu Lys Glu Phe Glu Thr Lys Ile Met Ala Ala Lys Glu Arg
                485                 490                 495
Ile Glu Glu Leu Glu Lys Glu Leu Phe Thr Ser Val Cys Glu Glu Val
            500                 505                 510
Lys Lys His Lys Glu Val Leu Leu Glu Ile Ser Glu Asp Leu Ala Lys
        515                 520                 525
Ile Asp Ala Leu Ser Thr Leu Ala Tyr Asp Ala Ile Met Tyr Asn Tyr
    530                 535                 540
Thr Lys Pro Val Phe Ser Glu Asp Arg Leu Glu Ile Lys Gly Gly Arg
545                 550                 555                 560
His Pro Val Val Glu Arg Phe Thr Gln Asn Phe Val Glu Asn Asp Ile
                565                 570                 575
Tyr Met Asp Asn Glu Lys Arg Phe Val Val Ile Thr Gly Pro Asn Met
            580                 585                 590
Ser Gly Lys Ser Thr Phe Ile Arg Gln Val Gly Leu Ile Ser Leu Met
        595                 600                 605
Ala Gln Ile Gly Ser Phe Val Pro Ala Gln Lys Ala Ile Leu Pro Val
    610                 615                 620
Phe Asp Arg Ile Phe Thr Arg Met Gly Ala Arg Asp Asp Leu Ala Gly
625                 630                 635                 640
Gly Arg Ser Thr Phe Leu Val Glu Met Asn Glu Met Ala Leu Ile Leu
                645                 650                 655
Leu Lys Ser Thr Asn Lys Ser Leu Val Leu Leu Asp Glu Val Gly Arg
            660                 665                 670
Gly Thr Ser Thr Gln Asp Gly Val Ser Ile Ala Trp Ala Ile Ser Glu
        675                 680                 685
Glu Leu Ile Lys Arg Gly Cys Lys Val Leu Phe Ala Thr His Phe Thr
    690                 695                 700
Glu Leu Thr Glu Leu Glu Lys His Phe Pro Gln Val Gln Asn Lys Thr
705                 710                 715                 720
Ile Leu Val Lys Glu Glu Gly Lys Asn Val Ile Phe Thr His Lys Val
                725                 730                 735
Val Asp Gly Val Ala Asp Arg Ser Tyr Gly Ile Glu Val Ala Lys Ile
            740                 745                 750
Ala Gly Ile Pro Asp Arg Val Ile Asn Arg Ala Tyr Glu Ile Leu Glu
        755                 760                 765
Arg Asn Phe Lys Asn Asn Thr Lys Lys Asn Gly Lys Ser Asn Arg Phe
    770                 775                 780
Ser Gln Gln Ile Pro Leu Phe Pro Val
785                 790
