                         SEQUENCE LISTING

<110>  Sandoz AG
 
<120>  Method for producing a recombinant protein with reduced 
       impurities

<130>  HEX-010 PCT

<150>  EP 15190359.8
<151>  2015-10-19

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  175
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant form of human G-CSF with N-terminal methionine

<400>  1

Met Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  2
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  2

Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln 
            20                  25                  30          


Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys 
    50                  55                  60                  


Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser 
65                  70                  75                  80  


Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp 
            100                 105                 110         


Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro 
        115                 120                 125             


Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 


<210>  3
<211>  175
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus sequence comprising sequence of natural human as well 
       as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  3

Xaa Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu 
            20                  25                  30          


Gln Glu Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser 
    50                  55                  60                  


Cys Pro Ser Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His 
65                  70                  75                  80  


Ser Gly Leu Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala 
            100                 105                 110         


Asp Phe Ala Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala 
        115                 120                 125             


Pro Ala Leu Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
                165                 170                 175 


<210>  4
<211>  204
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Gly Pro Ala Thr Gln Ser Pro Met Lys Leu Met Ala Leu Gln 
1               5                   10                  15      


Leu Leu Leu Trp His Ser Ala Leu Trp Thr Val Gln Glu Ala Thr Pro 
            20                  25                  30          


Leu Gly Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Cys Leu 
        35                  40                  45              


Glu Gln Val Arg Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu Lys 
    50                  55                  60                  


Leu Cys Ala Thr Tyr Lys Leu Cys His Pro Glu Glu Leu Val Leu Leu 
65                  70                  75                  80  


Gly His Ser Leu Gly Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser 
                85                  90                  95      


Gln Ala Leu Gln Leu Ala Gly Cys Leu Ser Gln Leu His Ser Gly Leu 
            100                 105                 110         


Phe Leu Tyr Gln Gly Leu Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu 
        115                 120                 125             


Leu Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val Ala Asp Phe Ala 
    130                 135                 140                 


Thr Thr Ile Trp Gln Gln Met Glu Glu Leu Gly Met Ala Pro Ala Leu 
145                 150                 155                 160 


Gln Pro Thr Gln Gly Ala Met Pro Ala Phe Ala Ser Ala Phe Gln Arg 
                165                 170                 175     


Arg Ala Gly Gly Val Leu Val Ala Ser His Leu Gln Ser Phe Leu Glu 
            180                 185                 190         


Val Ser Tyr Arg Val Leu Arg His Leu Ala Gln Pro 
        195                 200                 


<210>  5
<211>  2
<212>  PRT
<213>  Homo sapiens

<400>  5

Thr Pro 
1       


<210>  6
<211>  3
<212>  PRT
<213>  Homo sapiens

<400>  6

Thr Pro Leu 
1           


<210>  7
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  7

Thr Pro Leu Gly 
1               


<210>  8
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  8

Thr Pro Leu Gly Pro 
1               5   


<210>  9
<211>  6
<212>  PRT
<213>  Homo sapiens

<400>  9

Thr Pro Leu Gly Pro Ala 
1               5       


<210>  10
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  3 N-terminal amino acids of recombinant G-CSF

<400>  10

Met Thr Pro 
1           


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4 N-terminal amino acids of recombinant G-CSF

<400>  11

Met Thr Pro Leu 
1               


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5 N-terminal amino acids of recombinant G-CSF

<400>  12

Met Thr Pro Leu Gly 
1               5   


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6 N-terminal amino acids of recombinant G-CSF

<400>  13

Met Thr Pro Leu Gly Pro 
1               5       


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7 N-terminal amino acids of recombinant G-CSF

<400>  14

Met Thr Pro Leu Gly Pro Ala 
1               5           


<210>  15
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Consensus sequence comprising N-terminal sequence of natural 
       human as well as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  15

Xaa Thr Pro 
1           


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence comprising N-terminal sequence of natural 
       human as well as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  16

Xaa Thr Pro Leu 
1               


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence comprising N-terminal sequence of natural 
       human as well as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  17

Xaa Thr Pro Leu Gly 
1               5   


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence comprising N-terminal sequence of natural 
       human as well as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  18

Xaa Thr Pro Leu Gly Pro 
1               5       


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus sequence comprising N-terminal sequence of natural 
       human as well as recombinant human G-CSF


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa may be Met or absent

<400>  19

Xaa Thr Pro Leu Gly Pro Ala 
1               5           


