                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
 
<120>  Non-Human Animals Having a Humanized Lymphocyte-Activation Gene 3

<130>  34031 (10212US01)

<150>  62/370,430
<151>  2016-08-03

<150>  62/258,181
<151>  2015-11-20

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  1817
<212>  DNA
<213>  Rattus norvegicus

<400>  1
acattctttg cctcacctcc ctccttgtgg aatttctctc tctctctctc tttttttttt       60

ctcccaggac ctttttctga actcccttgc agggcctgtg aagcccgggg gccacagagg      120

agatgaggca ggatctgttc cttgaccttt tgcttctgca gctgctttgg gaagctccag      180

ttgtgtcttc agggcctggg aaagagctct ccgtggtgtg ggcccaggag ggagctcctg      240

tccatcttcc ctgcagcctc gaatttcccc acctggatcc caactttctg cgaagaggat      300

gggtcacctg gcaacatcga ccagacagtg accaacccgc ttccatcccg gcccttgacc      360

ttctccaggg aatgccctcg actaggagac acccacccca tcgctacacg gtgctgagtg      420

tggctccagg aggcctgcgc agcgggaggc agcccctgct atcccacgtg cagctggaga      480

agcgtggccc ccagcgcggg gacttctctc tgtggttgcg cccagctacg cgcaaagatg      540

cgggcgagta ccacgccttc gtgcgcctcc cggaccgcga cttctcctgc agcctccgcc      600

tgcgcgtcgg ccaggcctcg atgattgcca gtcccccagg aaccctcaag ccgtctgatt      660

gggtcatttt gaactgctcc ttcagtcgtc ctgaccgccc agtctctgtg cactggttcc      720

agggccaaag ccgagtgccc gtccacaatt caccccgtca ttatctagct gaaagtttcc      780

tcttactgcc ccaagtcagc ccactggatt ccgggacctg gggctgtgtc ctcacctaca      840

gagatggctt caatgtctcc atcacgtaca acctcaaggt tcagggtctg gaacctgtag      900

cccctttgac agtgtacgct gctgaaggtt ctagggtgga gctgccctgt cacttgcctc      960

ccgttgtggg gaccccttct ttgctcattg ccaagtggac tcctcctggg ggaggtcctg     1020

agctcccggt gactggaaag agtggcaatt ttacccttca acttgagaat gtgggtcggg     1080

cacaggctgg gacctacacc tgcagcatcc atctgcaggg gcggcagctc agtgcggctg     1140

tgacgttggc agtcatcaca gtgactccta aatccttcgg gttacctggc tccccgcaga     1200

agctgttatg tgaggtagtc ccggcatctg gagaaggaag atttgtgtgg cgccccctca     1260

gcgatctgtc caggagttcc ctgggccctg tgctggagtt gcaggaggcc aagcttctgg     1320

ctgagcaatg gcagtgtcag ctgtatgagg gccagaaact tcttggagca acagtgtaca     1380

ccgcagagtc tagctcaggc gcctggagtg ctaagagaat ctcaggtgac cttaaaggag     1440

gccatctctt cctctctctc atccttggtg cccttgcctt gttcctcttg gtgaccgggg     1500

cctttggctt tcacctgtgg agaagacagt tgctacggag aagattttct gccttagagc     1560

atgggattcg cccacctccg gttcagagta agatagagga gctggagcga gaaccggaga     1620

ccgagatgga accagagaca gagcccgatc cggagcctca gccggagccc gagctggaac     1680

cagagtccag gcagctctga ccaggagctg agacagccag cagcaggtct cagcagctct     1740

gcccgcccgc ccgcctgccc gcccgccgga ataaactccc tgtcagcagc aaaaaaaaaa     1800

aaaaaaaaaa aaaaaaa                                                    1817


<210>  2
<211>  525
<212>  PRT
<213>  Rattus norvegicus

<400>  2

Met Arg Gln Asp Leu Phe Leu Asp Leu Leu Leu Leu Gln Leu Leu Trp 
1               5                   10                  15      


Glu Ala Pro Val Val Ser Ser Gly Pro Gly Lys Glu Leu Ser Val Val 
            20                  25                  30          


Trp Ala Gln Glu Gly Ala Pro Val His Leu Pro Cys Ser Leu Glu Phe 
        35                  40                  45              


Pro His Leu Asp Pro Asn Phe Leu Arg Arg Gly Trp Val Thr Trp Gln 
    50                  55                  60                  


His Arg Pro Asp Ser Asp Gln Pro Ala Ser Ile Pro Ala Leu Asp Leu 
65                  70                  75                  80  


Leu Gln Gly Met Pro Ser Thr Arg Arg His Pro Pro His Arg Tyr Thr 
                85                  90                  95      


Val Leu Ser Val Ala Pro Gly Gly Leu Arg Ser Gly Arg Gln Pro Leu 
            100                 105                 110         


Leu Ser His Val Gln Leu Glu Lys Arg Gly Pro Gln Arg Gly Asp Phe 
        115                 120                 125             


Ser Leu Trp Leu Arg Pro Ala Thr Arg Lys Asp Ala Gly Glu Tyr His 
    130                 135                 140                 


Ala Phe Val Arg Leu Pro Asp Arg Asp Phe Ser Cys Ser Leu Arg Leu 
145                 150                 155                 160 


Arg Val Gly Gln Ala Ser Met Ile Ala Ser Pro Pro Gly Thr Leu Lys 
                165                 170                 175     


Pro Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg 
            180                 185                 190         


Pro Val Ser Val His Trp Phe Gln Gly Gln Ser Arg Val Pro Val His 
        195                 200                 205             


Asn Ser Pro Arg His Tyr Leu Ala Glu Ser Phe Leu Leu Leu Pro Gln 
    210                 215                 220                 


Val Ser Pro Leu Asp Ser Gly Thr Trp Gly Cys Val Leu Thr Tyr Arg 
225                 230                 235                 240 


Asp Gly Phe Asn Val Ser Ile Thr Tyr Asn Leu Lys Val Gln Gly Leu 
                245                 250                 255     


Glu Pro Val Ala Pro Leu Thr Val Tyr Ala Ala Glu Gly Ser Arg Val 
            260                 265                 270         


Glu Leu Pro Cys His Leu Pro Pro Val Val Gly Thr Pro Ser Leu Leu 
        275                 280                 285             


Ile Ala Lys Trp Thr Pro Pro Gly Gly Gly Pro Glu Leu Pro Val Thr 
    290                 295                 300                 


Gly Lys Ser Gly Asn Phe Thr Leu Gln Leu Glu Asn Val Gly Arg Ala 
305                 310                 315                 320 


Gln Ala Gly Thr Tyr Thr Cys Ser Ile His Leu Gln Gly Arg Gln Leu 
                325                 330                 335     


Ser Ala Ala Val Thr Leu Ala Val Ile Thr Val Thr Pro Lys Ser Phe 
            340                 345                 350         


Gly Leu Pro Gly Ser Pro Gln Lys Leu Leu Cys Glu Val Val Pro Ala 
        355                 360                 365             


Ser Gly Glu Gly Arg Phe Val Trp Arg Pro Leu Ser Asp Leu Ser Arg 
    370                 375                 380                 


Ser Ser Leu Gly Pro Val Leu Glu Leu Gln Glu Ala Lys Leu Leu Ala 
385                 390                 395                 400 


Glu Gln Trp Gln Cys Gln Leu Tyr Glu Gly Gln Lys Leu Leu Gly Ala 
                405                 410                 415     


Thr Val Tyr Thr Ala Glu Ser Ser Ser Gly Ala Trp Ser Ala Lys Arg 
            420                 425                 430         


Ile Ser Gly Asp Leu Lys Gly Gly His Leu Phe Leu Ser Leu Ile Leu 
        435                 440                 445             


Gly Ala Leu Ala Leu Phe Leu Leu Val Thr Gly Ala Phe Gly Phe His 
    450                 455                 460                 


Leu Trp Arg Arg Gln Leu Leu Arg Arg Arg Phe Ser Ala Leu Glu His 
465                 470                 475                 480 


Gly Ile Arg Pro Pro Pro Val Gln Ser Lys Ile Glu Glu Leu Glu Arg 
                485                 490                 495     


Glu Pro Glu Thr Glu Met Glu Pro Glu Thr Glu Pro Asp Pro Glu Pro 
            500                 505                 510         


Gln Pro Glu Pro Glu Leu Glu Pro Glu Ser Arg Gln Leu 
        515                 520                 525 


<210>  3
<211>  2020
<212>  DNA
<213>  Mus musculus

<400>  3
gggcagtggg gaggagaagc agaaggactg ggtctggagg agcagctcaa gttctagcta       60

gctgcagtgg gtttgcctgc actctgctct gggtcccagc ccgggcctct gatcattatc      120

catcctgctg tctccagtcc ccactcctgg ggcgtcctct tcaccctaca ttctttccct      180

ccgcctcacc tcctccttgt agaacttctc tctctctctc tctctctctc tctctctctc      240

tctctctctc tctgtgtgtg tgtgtgtgtc tgtctgtctg tctgtctctc tctcctccca      300

ggaccttttt ctaacctccc ttggagggct ggggaggccc gggccataga ggagatgagg      360

gaggacctgc tccttggctt tttgcttctg ggactgcttt gggaagctcc agttgtgtct      420

tcagggcctg ggaaagagct ccccgtggtg tgggcccagg agggagctcc cgtccatctt      480

ccctgcagcc tcaaatcccc caacctggat cctaactttc tacgaagagg aggggttatc      540

tggcaacatc aaccagacag tggccaaccc actcccatcc cggcccttga ccttcaccag      600

gggatgccct cgcctagaca acccgcaccc ggtcgctaca cggtgctgag cgtggctcca      660

ggaggcctgc gcagcgggag gcagcccctg catccccacg tgcagctgga ggagcgcggc      720

ctccagcgcg gggacttctc tctgtggttg cgcccagctc tgcgcaccga tgcgggcgag      780

taccacgcca ccgtgcgcct cccgaaccgc gccctctcct gcagtctccg cctgcgcgtc      840

ggccaggcct cgatgattgc tagtccctca ggagtcctca agctgtctga ttgggtcctt      900

ttgaactgct ccttcagccg tcctgaccgc ccagtctctg tgcactggtt ccagggccag      960

aaccgagtgc ctgtctacaa ctcaccgcgt cattttttag ctgaaacttt cctgttactg     1020

ccccaagtca gccccctgga ctctgggacc tggggctgtg tcctcaccta cagagatggc     1080

ttcaatgtct ccatcacgta caacctcaag gttctgggtc tggagcccgt agcccctctg     1140

acagtgtacg ctgctgaagg ttctagggtg gagctgccct gtcatttgcc cccaggagtg     1200

gggacccctt ctttgctcat tgccaagtgg actcctcctg gaggaggtcc tgagctcccc     1260

gtggctggaa agagtggcaa ttttaccctt caccttgagg ctgtgggtct ggcacaggct     1320

gggacctaca cctgtagcat ccatctgcag ggacagcagc tcaatgccac tgtcacgttg     1380

gcggtcatca cagtgactcc caaatccttc gggttacctg gctcccgggg gaagctgttg     1440

tgtgaggtaa ccccggcatc tggaaaggaa agatttgtgt ggcgtcccct gaacaatctg     1500

tccaggagtt gcccgggccc tgtgctggag attcaggagg ccaggctcct tgctgagcga     1560

tggcagtgtc agctgtacga gggccagagg cttcttggag cgacagtgta cgccgcagag     1620

tctagctcag gcgcccacag tgctaggaga atctcaggtg accttaaagg aggccatctc     1680

gttctcgttc tcatccttgg tgccctctcc ctgttccttt tggtggccgg ggcctttggc     1740

tttcactggt ggagaaaaca gttgctactg agaagatttt ctgccttaga acatgggatt     1800

cagccatttc cggctcagag gaagatagag gagctggagc gagaactgga gacggagatg     1860

ggacaggagc cggagcccga gccggagcca cagctggagc cagagcccag gcagctctga     1920

cctggagccg aggcagccag caggtctcag cagctccgcc cgcccgcccg cccgcccgaa     1980

taaactccct gtcagcagca tcaaaaaaaa aaaaaaaaaa                           2020


<210>  4
<211>  521
<212>  PRT
<213>  Mus musculus

<400>  4

Met Arg Glu Asp Leu Leu Leu Gly Phe Leu Leu Leu Gly Leu Leu Trp 
1               5                   10                  15      


Glu Ala Pro Val Val Ser Ser Gly Pro Gly Lys Glu Leu Pro Val Val 
            20                  25                  30          


Trp Ala Gln Glu Gly Ala Pro Val His Leu Pro Cys Ser Leu Lys Ser 
        35                  40                  45              


Pro Asn Leu Asp Pro Asn Phe Leu Arg Arg Gly Gly Val Ile Trp Gln 
    50                  55                  60                  


His Gln Pro Asp Ser Gly Gln Pro Thr Pro Ile Pro Ala Leu Asp Leu 
65                  70                  75                  80  


His Gln Gly Met Pro Ser Pro Arg Gln Pro Ala Pro Gly Arg Tyr Thr 
                85                  90                  95      


Val Leu Ser Val Ala Pro Gly Gly Leu Arg Ser Gly Arg Gln Pro Leu 
            100                 105                 110         


His Pro His Val Gln Leu Glu Glu Arg Gly Leu Gln Arg Gly Asp Phe 
        115                 120                 125             


Ser Leu Trp Leu Arg Pro Ala Leu Arg Thr Asp Ala Gly Glu Tyr His 
    130                 135                 140                 


Ala Thr Val Arg Leu Pro Asn Arg Ala Leu Ser Cys Ser Leu Arg Leu 
145                 150                 155                 160 


Arg Val Gly Gln Ala Ser Met Ile Ala Ser Pro Ser Gly Val Leu Lys 
                165                 170                 175     


Leu Ser Asp Trp Val Leu Leu Asn Cys Ser Phe Ser Arg Pro Asp Arg 
            180                 185                 190         


Pro Val Ser Val His Trp Phe Gln Gly Gln Asn Arg Val Pro Val Tyr 
        195                 200                 205             


Asn Ser Pro Arg His Phe Leu Ala Glu Thr Phe Leu Leu Leu Pro Gln 
    210                 215                 220                 


Val Ser Pro Leu Asp Ser Gly Thr Trp Gly Cys Val Leu Thr Tyr Arg 
225                 230                 235                 240 


Asp Gly Phe Asn Val Ser Ile Thr Tyr Asn Leu Lys Val Leu Gly Leu 
                245                 250                 255     


Glu Pro Val Ala Pro Leu Thr Val Tyr Ala Ala Glu Gly Ser Arg Val 
            260                 265                 270         


Glu Leu Pro Cys His Leu Pro Pro Gly Val Gly Thr Pro Ser Leu Leu 
        275                 280                 285             


Ile Ala Lys Trp Thr Pro Pro Gly Gly Gly Pro Glu Leu Pro Val Ala 
    290                 295                 300                 


Gly Lys Ser Gly Asn Phe Thr Leu His Leu Glu Ala Val Gly Leu Ala 
305                 310                 315                 320 


Gln Ala Gly Thr Tyr Thr Cys Ser Ile His Leu Gln Gly Gln Gln Leu 
                325                 330                 335     


Asn Ala Thr Val Thr Leu Ala Val Ile Thr Val Thr Pro Lys Ser Phe 
            340                 345                 350         


Gly Leu Pro Gly Ser Arg Gly Lys Leu Leu Cys Glu Val Thr Pro Ala 
        355                 360                 365             


Ser Gly Lys Glu Arg Phe Val Trp Arg Pro Leu Asn Asn Leu Ser Arg 
    370                 375                 380                 


Ser Cys Pro Gly Pro Val Leu Glu Ile Gln Glu Ala Arg Leu Leu Ala 
385                 390                 395                 400 


Glu Arg Trp Gln Cys Gln Leu Tyr Glu Gly Gln Arg Leu Leu Gly Ala 
                405                 410                 415     


Thr Val Tyr Ala Ala Glu Ser Ser Ser Gly Ala His Ser Ala Arg Arg 
            420                 425                 430         


Ile Ser Gly Asp Leu Lys Gly Gly His Leu Val Leu Val Leu Ile Leu 
        435                 440                 445             


Gly Ala Leu Ser Leu Phe Leu Leu Val Ala Gly Ala Phe Gly Phe His 
    450                 455                 460                 


Trp Trp Arg Lys Gln Leu Leu Leu Arg Arg Phe Ser Ala Leu Glu His 
465                 470                 475                 480 


Gly Ile Gln Pro Phe Pro Ala Gln Arg Lys Ile Glu Glu Leu Glu Arg 
                485                 490                 495     


Glu Leu Glu Thr Glu Met Gly Gln Glu Pro Glu Pro Glu Pro Glu Pro 
            500                 505                 510         


Gln Leu Glu Pro Glu Pro Arg Gln Leu 
        515                 520     


<210>  5
<211>  1995
<212>  DNA
<213>  Homo sapiens

<400>  5
acaggggtga aggcccagag accagcagaa cggcatccca gccacgacgg ccactttgct       60

ctgtctgctc tccgccacgg ccctgctctg ttccctggga cacccccgcc cccacctcct      120

caggctgcct gatctgccca gctttccagc tttcctctgg attccggcct ctggtcatcc      180

ctccccaccc tctctccaag gccctctcct ggtctccctt cttctagaac cccttcctcc      240

acctccctct ctgcagaact tctcctttac cccccacccc ccaccactgc cccctttcct      300

tttctgacct ccttttggag ggctcagcgc tgcccagacc ataggagaga tgtgggaggc      360

tcagttcctg ggcttgctgt ttctgcagcc gctttgggtg gctccagtga agcctctcca      420

gccaggggct gaggtcccgg tggtgtgggc ccaggagggg gctcctgccc agctcccctg      480

cagccccaca atccccctcc aggatctcag ccttctgcga agagcagggg tcacttggca      540

gcatcagcca gacagtggcc cgcccgctgc cgcccccggc catcccctgg cccccggccc      600

tcacccggcg gcgccctcct cctgggggcc caggccccgc cgctacacgg tgctgagcgt      660

gggtcccgga ggcctgcgca gcgggaggct gcccctgcag ccccgcgtcc agctggatga      720

gcgcggccgg cagcgcgggg acttctcgct atggctgcgc ccagcccggc gcgcggacgc      780

cggcgagtac cgcgccgcgg tgcacctcag ggaccgcgcc ctctcctgcc gcctccgtct      840

gcgcctgggc caggcctcga tgactgccag ccccccagga tctctcagag cctccgactg      900

ggtcattttg aactgctcct tcagccgccc tgaccgccca gcctctgtgc attggttccg      960

gaaccggggc cagggccgag tccctgtccg ggagtccccc catcaccact tagcggaaag     1020

cttcctcttc ctgccccaag tcagccccat ggactctggg ccctggggct gcatcctcac     1080

ctacagagat ggcttcaacg tctccatcat gtataacctc actgttctgg gtctggagcc     1140

cccaactccc ttgacagtgt acgctggagc aggttccagg gtggggctgc cctgccgcct     1200

gcctgctggt gtggggaccc ggtctttcct cactgccaag tggactcctc ctgggggagg     1260

ccctgacctc ctggtgactg gagacaatgg cgactttacc cttcgactag aggatgtgag     1320

ccaggcccag gctgggacct acacctgcca tatccatctg caggaacagc agctcaatgc     1380

cactgtcaca ttggcaatca tcacagtgac tcccaaatcc tttgggtcac ctggatccct     1440

ggggaagctg ctttgtgagg tgactccagt atctggacaa gaacgctttg tgtggagctc     1500

tctggacacc ccatcccaga ggagtttctc aggaccttgg ctggaggcac aggaggccca     1560

gctcctttcc cagccttggc aatgccagct gtaccagggg gagaggcttc ttggagcagc     1620

agtgtacttc acagagctgt ctagcccagg tgcccaacgc tctgggagag ccccaggtgc     1680

cctcccagca ggccacctcc tgctgtttct catccttggt gtcctttctc tgctcctttt     1740

ggtgactgga gcctttggct ttcacctttg gagaagacag tggcgaccaa gacgattttc     1800

tgccttagag caagggattc accctccgca ggctcagagc aagatagagg agctggagca     1860

agaaccggag ccggagccgg agccggaacc ggagcccgag cccgagcccg agccggagca     1920

gctctgacct ggagctgagg cagccagcag atctcagcag cccagtccaa ataaactccc     1980

tgtcagcagc aaaaa                                                      1995


<210>  6
<211>  525
<212>  PRT
<213>  Homo sapiens

<400>  6

Met Trp Glu Ala Gln Phe Leu Gly Leu Leu Phe Leu Gln Pro Leu Trp 
1               5                   10                  15      


Val Ala Pro Val Lys Pro Leu Gln Pro Gly Ala Glu Val Pro Val Val 
            20                  25                  30          


Trp Ala Gln Glu Gly Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile 
        35                  40                  45              


Pro Leu Gln Asp Leu Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln 
    50                  55                  60                  


His Gln Pro Asp Ser Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu 
65                  70                  75                  80  


Ala Pro Gly Pro His Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro 
                85                  90                  95      


Arg Arg Tyr Thr Val Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly 
            100                 105                 110         


Arg Leu Pro Leu Gln Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln 
        115                 120                 125             


Arg Gly Asp Phe Ser Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala 
    130                 135                 140                 


Gly Glu Tyr Arg Ala Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys 
145                 150                 155                 160 


Arg Leu Arg Leu Arg Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro 
                165                 170                 175     


Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser 
            180                 185                 190         


Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln 
        195                 200                 205             


Gly Arg Val Pro Val Arg Glu Ser Pro His His His Leu Ala Glu Ser 
    210                 215                 220                 


Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly 
225                 230                 235                 240 


Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn 
                245                 250                 255     


Leu Thr Val Leu Gly Leu Glu Pro Pro Thr Pro Leu Thr Val Tyr Ala 
            260                 265                 270         


Gly Ala Gly Ser Arg Val Gly Leu Pro Cys Arg Leu Pro Ala Gly Val 
        275                 280                 285             


Gly Thr Arg Ser Phe Leu Thr Ala Lys Trp Thr Pro Pro Gly Gly Gly 
    290                 295                 300                 


Pro Asp Leu Leu Val Thr Gly Asp Asn Gly Asp Phe Thr Leu Arg Leu 
305                 310                 315                 320 


Glu Asp Val Ser Gln Ala Gln Ala Gly Thr Tyr Thr Cys His Ile His 
                325                 330                 335     


Leu Gln Glu Gln Gln Leu Asn Ala Thr Val Thr Leu Ala Ile Ile Thr 
            340                 345                 350         


Val Thr Pro Lys Ser Phe Gly Ser Pro Gly Ser Leu Gly Lys Leu Leu 
        355                 360                 365             


Cys Glu Val Thr Pro Val Ser Gly Gln Glu Arg Phe Val Trp Ser Ser 
    370                 375                 380                 


Leu Asp Thr Pro Ser Gln Arg Ser Phe Ser Gly Pro Trp Leu Glu Ala 
385                 390                 395                 400 


Gln Glu Ala Gln Leu Leu Ser Gln Pro Trp Gln Cys Gln Leu Tyr Gln 
                405                 410                 415     


Gly Glu Arg Leu Leu Gly Ala Ala Val Tyr Phe Thr Glu Leu Ser Ser 
            420                 425                 430         


Pro Gly Ala Gln Arg Ser Gly Arg Ala Pro Gly Ala Leu Pro Ala Gly 
        435                 440                 445             


His Leu Leu Leu Phe Leu Ile Leu Gly Val Leu Ser Leu Leu Leu Leu 
    450                 455                 460                 


Val Thr Gly Ala Phe Gly Phe His Leu Trp Arg Arg Gln Trp Arg Pro 
465                 470                 475                 480 


Arg Arg Phe Ser Ala Leu Glu Gln Gly Ile His Pro Pro Gln Ala Gln 
                485                 490                 495     


Ser Lys Ile Glu Glu Leu Glu Gln Glu Pro Glu Pro Glu Pro Glu Pro 
            500                 505                 510         


Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Gln Leu 
        515                 520                 525 


<210>  7
<211>  2038
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary humanized Lag-3 nucleic acid sequence

<400>  7
gggcagtggg gaggagaagc agaaggactg ggtctggagg agcagctcaa gttctagcta       60

gctgcagtgg gtttgcctgc actctgctct gggtcccagc ccgggcctct gatcattatc      120

catcctgctg tctccagtcc ccactcctgg ggcgtcctct tcaccctaca ttctttccct      180

ccgcctcacc tcctccttgt agaacttctc tctctctctc tctctctctc tctctctctc      240

tctctctctc tctgtgtgtg tgtgtgtgtc tgtctgtctg tctgtctctc tctcctccca      300

ggaccttttt ctaacctccc ttggagggct ggggaggccc gggccataga ggagatgagg      360

gaggacctgc tccttggctt tttgcttctg ggactgcttt gggaagctcc agtgaagcct      420

ctccagccag gggctgaggt cccggtggtg tgggcccagg agggggctcc tgcccagctc      480

ccctgcagcc ccacaatccc cctccaggat ctcagccttc tgcgaagagc aggggtcact      540

tggcagcatc agccagacag tggcccgccc gctgccgccc ccggccatcc cctggccccc      600

ggccctcacc cggcggcgcc ctcctcctgg gggcccaggc cccgccgcta cacggtgctg      660

agcgtgggtc ccggaggcct gcgcagcggg aggctgcccc tgcagccccg cgtccagctg      720

gatgagcgcg gccggcagcg cggggacttc tcgctatggc tgcgcccagc ccggcgcgcg      780

gacgccggcg agtaccgcgc cgcggtgcac ctcagggacc gcgccctctc ctgccgcctc      840

cgtctgcgcc tgggccaggc ctcgatgact gccagccccc caggatctct cagagcctcc      900

gactgggtca ttttgaactg ctccttcagc cgccctgacc gcccagcctc tgtgcattgg      960

ttccggaacc ggggccaggg ccgagtccct gtccgggagt ccccccatca ccacttagcg     1020

gaaagcttcc tcttcctgcc ccaagtcagc cccatggact ctgggccctg gggctgcatc     1080

ctcacctaca gagatggctt caacgtctcc atcatgtata acctcactgt tctgggtctg     1140

gagcccgtag cccctctgac agtgtacgct gctgaaggtt ctagggtgga gctgccctgt     1200

catttgcccc caggagtggg gaccccttct ttgctcattg ccaagtggac tcctcctgga     1260

ggaggtcctg agctccccgt ggctggaaag agtggcaatt ttacccttca ccttgaggct     1320

gtgggtctgg cacaggctgg gacctacacc tgtagcatcc atctgcaggg acagcagctc     1380

aatgccactg tcacgttggc ggtcatcaca gtgactccca aatccttcgg gttacctggc     1440

tcccggggga agctgttgtg tgaggtaacc ccggcatctg gaaaggaaag atttgtgtgg     1500

cgtcccctga acaatctgtc caggagttgc ccgggccctg tgctggagat tcaggaggcc     1560

aggctccttg ctgagcgatg gcagtgtcag ctgtacgagg gccagaggct tcttggagcg     1620

acagtgtacg ccgcagagtc tagctcaggc gcccacagtg ctaggagaat ctcaggtgac     1680

cttaaaggag gccatctcgt tctcgttctc atccttggtg ccctctccct gttccttttg     1740

gtggccgggg cctttggctt tcactggtgg agaaaacagt tgctactgag aagattttct     1800

gccttagaac atgggattca gccatttccg gctcagagga agatagagga gctggagcga     1860

gaactggaga cggagatggg acaggagccg gagcccgagc cggagccaca gctggagcca     1920

gagcccaggc agctctgacc tggagccgag gcagccagca ggtctcagca gctccgcccg     1980

cccgcccgcc cgcccgaata aactccctgt cagcagcatc aaaaaaaaaa aaaaaaaa       2038


<210>  8
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  exemplary humanized Lag-3 amino acid sequence

<400>  8

Met Arg Glu Asp Leu Leu Leu Gly Phe Leu Leu Leu Gly Leu Leu Trp 
1               5                   10                  15      


Glu Ala Pro Val Lys Pro Leu Gln Pro Gly Ala Glu Val Pro Val Val 
            20                  25                  30          


Trp Ala Gln Glu Gly Ala Pro Ala Gln Leu Pro Cys Ser Pro Thr Ile 
        35                  40                  45              


Pro Leu Gln Asp Leu Ser Leu Leu Arg Arg Ala Gly Val Thr Trp Gln 
    50                  55                  60                  


His Gln Pro Asp Ser Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu 
65                  70                  75                  80  


Ala Pro Gly Pro His Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro 
                85                  90                  95      


Arg Arg Tyr Thr Val Leu Ser Val Gly Pro Gly Gly Leu Arg Ser Gly 
            100                 105                 110         


Arg Leu Pro Leu Gln Pro Arg Val Gln Leu Asp Glu Arg Gly Arg Gln 
        115                 120                 125             


Arg Gly Asp Phe Ser Leu Trp Leu Arg Pro Ala Arg Arg Ala Asp Ala 
    130                 135                 140                 


Gly Glu Tyr Arg Ala Ala Val His Leu Arg Asp Arg Ala Leu Ser Cys 
145                 150                 155                 160 


Arg Leu Arg Leu Arg Leu Gly Gln Ala Ser Met Thr Ala Ser Pro Pro 
                165                 170                 175     


Gly Ser Leu Arg Ala Ser Asp Trp Val Ile Leu Asn Cys Ser Phe Ser 
            180                 185                 190         


Arg Pro Asp Arg Pro Ala Ser Val His Trp Phe Arg Asn Arg Gly Gln 
        195                 200                 205             


Gly Arg Val Pro Val Arg Glu Ser Pro His His His Leu Ala Glu Ser 
    210                 215                 220                 


Phe Leu Phe Leu Pro Gln Val Ser Pro Met Asp Ser Gly Pro Trp Gly 
225                 230                 235                 240 


Cys Ile Leu Thr Tyr Arg Asp Gly Phe Asn Val Ser Ile Met Tyr Asn 
                245                 250                 255     


Leu Thr Val Leu Gly Leu Glu Pro Val Ala Pro Leu Thr Val Tyr Ala 
            260                 265                 270         


Ala Glu Gly Ser Arg Val Glu Leu Pro Cys His Leu Pro Pro Gly Val 
        275                 280                 285             


Gly Thr Pro Ser Leu Leu Ile Ala Lys Trp Thr Pro Pro Gly Gly Gly 
    290                 295                 300                 


Pro Glu Leu Pro Val Ala Gly Lys Ser Gly Asn Phe Thr Leu His Leu 
305                 310                 315                 320 


Glu Ala Val Gly Leu Ala Gln Ala Gly Thr Tyr Thr Cys Ser Ile His 
                325                 330                 335     


Leu Gln Gly Gln Gln Leu Asn Ala Thr Val Thr Leu Ala Val Ile Thr 
            340                 345                 350         


Val Thr Pro Lys Ser Phe Gly Leu Pro Gly Ser Arg Gly Lys Leu Leu 
        355                 360                 365             


Cys Glu Val Thr Pro Ala Ser Gly Lys Glu Arg Phe Val Trp Arg Pro 
    370                 375                 380                 


Leu Asn Asn Leu Ser Arg Ser Cys Pro Gly Pro Val Leu Glu Ile Gln 
385                 390                 395                 400 


Glu Ala Arg Leu Leu Ala Glu Arg Trp Gln Cys Gln Leu Tyr Glu Gly 
                405                 410                 415     


Gln Arg Leu Leu Gly Ala Thr Val Tyr Ala Ala Glu Ser Ser Ser Gly 
            420                 425                 430         


Ala His Ser Ala Arg Arg Ile Ser Gly Asp Leu Lys Gly Gly His Leu 
        435                 440                 445             


Val Leu Val Leu Ile Leu Gly Ala Leu Ser Leu Phe Leu Leu Val Ala 
    450                 455                 460                 


Gly Ala Phe Gly Phe His Trp Trp Arg Lys Gln Leu Leu Leu Arg Arg 
465                 470                 475                 480 


Phe Ser Ala Leu Glu His Gly Ile Gln Pro Phe Pro Ala Gln Arg Lys 
                485                 490                 495     


Ile Glu Glu Leu Glu Arg Glu Leu Glu Thr Glu Met Gly Gln Glu Pro 
            500                 505                 510         


Glu Pro Glu Pro Glu Pro Gln Leu Glu Pro Glu Pro Arg Gln Leu 
        515                 520                 525         


<210>  9
<211>  1741
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary synthetic DNA fragment for humanization (1,741 bp 
       including exons 2-4 and part of intron 4 of a human LAG3 gene

<400>  9
tgaagcctct ccagccaggg gctgaggtcc cggtggtgtg ggcccaggag ggggctcctg       60

cccagctccc ctgcagcccc acaatccccc tccaggatct cagccttctg cgaagagcag      120

gggtcacttg gcagcatcag ccagacaggt atgcacccca aacttgggca acaggacctc      180

cgaatccagc actcaacccc acacccgtgc cggtcctctg tcccctgccc tgaggtgtca      240

ctccctctga agccagtgac ccagtctccc tgccctcgct tgcaccgttc ctgcccttgc      300

tctgcaatca gcgaccctca cgccagcatc ccttctctcc agaagtggat gcggccagtc      360

caacagaggg gtcgggcgtg aggggacggt tggtggtcaa gagaactctt ggggcgggct      420

ttctcatcct caacgggtgg ctgcctgcat cctcccgggc ttcctacccc tggagcttct      480

caactccatt ctctttcccg cccagtggcc cgcccgctgc cgcccccggc catcccctgg      540

cccccggccc tcacccggcg gcgccctcct cctgggggcc caggccccgc cgctacacgg      600

tgctgagcgt gggtcccgga ggcctgcgca gcgggaggct gcccctgcag ccccgcgtcc      660

agctggatga gcgcggccgg cagcgcgggg acttctcgct atggctgcgc ccagcccggc      720

gcgcggacgc cggcgagtac cgcgccgcgg tgcacctcag ggaccgcgcc ctctcctgcc      780

gcctccgtct gcgcctgggc caggcctcga gtatgtgggg cgggacgatg ggagaagggc      840

tgggaggtgg gtccccatcc cctgcctccc gggacgcagg aagggctggg gcagaggctg      900

cgccctaggc cctgtcggag agctcccaga agagtagagg aagggggtgg gcggcctgct      960

ggagtggaag gtgcccccga agcacgtgta tggggggccc tgtggagaga ttgtgtcacc     1020

cccgagctcc ccttctccca cccacgcggg agtgcccaga gggaggggga gggggggaga     1080

gcatggggct aaagtgattc atttcagata tctgtagctc agggggtggg cttcgcgggg     1140

ttccaggcca ggaaaacggc aagggtggct gatgccaagt aaactccagg ccagggacgg     1200

ggaaagtggt cctggggagt cttggggatc cactttatgc acctccaggt gctggaagct     1260

gagatgggga gagggtgatg tgggagagga gaagacaagt ctaaagccag gtgcctgttt     1320

ccaggagctt ccggcttggc agccctgctg tgttgggaaa ttgtttccag tgggctgatg     1380

aagtcttctt tatccttgca cagtgactgc cagcccccca ggatctctca gagcctccga     1440

ctgggtcatt ttgaactgct ccttcagccg ccctgaccgc ccagcctctg tgcattggtt     1500

ccggaaccgg ggccagggcc gagtccctgt ccgggagtcc ccccatcacc acttagcgga     1560

aagcttcctc ttcctgcccc aagtcagccc catggactct gggccctggg gctgcatcct     1620

cacctacaga gatggcttca acgtctccat catgtataac ctcactgttc tgggtaactc     1680

ccccactctg cttcacattt gaccacaact ccttcctgcc ccccttgtca cctcccctaa     1740

c                                                                     1741


<210>  10
<211>  7522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary humanized Lag3 allele including a selection cassette

<400>  10
tgaagcctct ccagccaggg gctgaggtcc cggtggtgtg ggcccaggag ggggctcctg       60

cccagctccc ctgcagcccc acaatccccc tccaggatct cagccttctg cgaagagcag      120

gggtcacttg gcagcatcag ccagacaggt atgcacccca aacttgggca acaggacctc      180

cgaatccagc actcaacccc acacccgtgc cggtcctctg tcccctgccc tgaggtgtca      240

ctccctctga agccagtgac ccagtctccc tgccctcgct tgcaccgttc ctgcccttgc      300

tctgcaatca gcgaccctca cgccagcatc ccttctctcc agaagtggat gcggccagtc      360

caacagaggg gtcgggcgtg aggggacggt tggtggtcaa gagaactctt ggggcgggct      420

ttctcatcct caacgggtgg ctgcctgcat cctcccgggc ttcctacccc tggagcttct      480

caactccatt ctctttcccg cccagtggcc cgcccgctgc cgcccccggc catcccctgg      540

cccccggccc tcacccggcg gcgccctcct cctgggggcc caggccccgc cgctacacgg      600

tgctgagcgt gggtcccgga ggcctgcgca gcgggaggct gcccctgcag ccccgcgtcc      660

agctggatga gcgcggccgg cagcgcgggg acttctcgct atggctgcgc ccagcccggc      720

gcgcggacgc cggcgagtac cgcgccgcgg tgcacctcag ggaccgcgcc ctctcctgcc      780

gcctccgtct gcgcctgggc caggcctcga gtatgtgggg cgggacgatg ggagaagggc      840

tgggaggtgg gtccccatcc cctgcctccc gggacgcagg aagggctggg gcagaggctg      900

cgccctaggc cctgtcggag agctcccaga agagtagagg aagggggtgg gcggcctgct      960

ggagtggaag gtgcccccga agcacgtgta tggggggccc tgtggagaga ttgtgtcacc     1020

cccgagctcc ccttctccca cccacgcggg agtgcccaga gggaggggga gggggggaga     1080

gcatggggct aaagtgattc atttcagata tctgtagctc agggggtggg cttcgcgggg     1140

ttccaggcca ggaaaacggc aagggtggct gatgccaagt aaactccagg ccagggacgg     1200

ggaaagtggt cctggggagt cttggggatc cactttatgc acctccaggt gctggaagct     1260

gagatgggga gagggtgatg tgggagagga gaagacaagt ctaaagccag gtgcctgttt     1320

ccaggagctt ccggcttggc agccctgctg tgttgggaaa ttgtttccag tgggctgatg     1380

aagtcttctt tatccttgca cagtgactgc cagcccccca ggatctctca gagcctccga     1440

ctgggtcatt ttgaactgct ccttcagccg ccctgaccgc ccagcctctg tgcattggtt     1500

ccggaaccgg ggccagggcc gagtccctgt ccgggagtcc ccccatcacc acttagcgga     1560

aagcttcctc ttcctgcccc aagtcagccc catggactct gggccctggg gctgcatcct     1620

cacctacaga gatggcttca acgtctccat catgtataac ctcactgttc tgggtaactc     1680

ccccactctg cttcacattt gaccacaact ccttcctgcc ccccttgtca cctcccctaa     1740

cgtcgagata acttcgtata atgtatgcta tacgaagtta tatgcatggc ctccgcgccg     1800

ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga     1860

agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc     1920

ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg     1980

tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct     2040

tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga     2100

cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt     2160

tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc     2220

gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt     2280

ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg     2340

gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca     2400

aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga     2460

aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg     2520

tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg     2580

ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg     2640

ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc     2700

tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg     2760

acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct     2820

atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt     2880

tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc     2940

agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg     3000

tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa     3060

ctaaaccatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc cggccgcttg     3120

ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc     3180

cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg     3240

tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt     3300

tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg     3360

cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat     3420

catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca     3480

ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca     3540

ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa     3600

ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct gcttgccgaa     3660

tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc     3720

ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga     3780

atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc     3840

cttctatcgc cttcttgacg agttcttctg aggggatccg ctgtaagtct gcagaaattg     3900

atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt     3960

taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg     4020

ggggtggggt gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt     4080

atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca     4140

gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt     4200

ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag     4260

cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg     4320

ccaagttcta attccatcag acctcgacct gcagccccta gcccgggcgc cagtagcagc     4380

acccacgtcc accttctgtc tagtaatgtc caacacctcc ctcagtccaa acactgctct     4440

gcatccatgt ggctcccatt tatacctgaa gcacttgatg gggcctcaat gttttactag     4500

agcccacccc cctgcaactc tgagaccctc tggatttgtc tgtcagtgcc tcactggggc     4560

gttggataat ttcttaaaag gtcaagttcc ctcagcagca ttctctgagc agtctgaaga     4620

tgtgtgcttt tcacagttca aatccatgtg gctgtttcac ccacctgcct ggccttgggt     4680

tatctatcag gacctagcct agaagcaggt gtgtggcact taacacctaa gctgagtgac     4740

taactgaaca ctcaagtgga tgccatcttt gtcacttctt gactgtgaca caagcaactc     4800

ctgatgccaa agccctgccc acccctctca tgcccatatt tggacatggt acaggtcctc     4860

actggccatg gtctgtgagg tcctggtcct ctttgacttc ataattccta ggggccacta     4920

gtatctataa gaggaagagg gtgctggctc ccaggccaca gcccacaaaa ttccacctgc     4980

tcacaggttg gctggctcga cccaggtggt gtcccctgct ctgagccagc tcccggccaa     5040

gccagcacca tgggtacccc caagaagaag aggaaggtgc gtaccgattt aaattccaat     5100

ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt     5160

cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa     5220

atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg     5280

tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg     5340

gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg     5400

ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa     5460

aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac     5520

caggttcgtt cactcatgga aaatagtgat cgctgccagg atatacgtaa tctggcattt     5580

ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa     5640

gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg     5700

gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga     5760

tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga     5820

aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg     5880

atttttgaag caactcatcg attgatttac ggcgctaagg taaatataaa atttttaagt     5940

gtataatgtg ttaaactact gattctaatt gtttgtgtat tttaggatga ctctggtcag     6000

agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc     6060

gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc     6120

atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat     6180

ggcgattgat ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta     6240

cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt     6300

gttgttgtta aacctgccct agttgcggcc aattccagct gagcgtgcct ccgcaccatt     6360

accagttggt ctggtgtcaa aaataataat aaccgggcag gggggatcta agctctagat     6420

aagtaatgat cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc     6480

tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt     6540

ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag     6600

catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg     6660

tctggaataa cttcgtataa tgtatgctat acgaagttat gctagtaact ataacggtcc     6720

taaggtagcg agctagcgac ccccaaaact ttctcagctg cgtgtggtct cactccacat     6780

cactttgttt cagtgtccaa accattttct ctctgggcat cttttagctg ctgtctctct     6840

tacttttatt tatttatttg tgtgtttatt tatttatttt cattttagcg tgcgttggtg     6900

ttttgcctgc atagatgtct gtgtcagggt attggattcc ctggaacttg acctacagac     6960

agtcatgaga taccatatgg gtgctgggaa ttgaacccag ctcctctgga aggacagcca     7020

gtgttctaat ctgccatctc tcactgttta tcccttggct gttcagcctc ctgagccttt     7080

ggtctcttgc tgcctcagtt tccctagttt ctctgctttg ctctgtttct ttctgtgtta     7140

cagccaaatg cctccttccc ccttctgcct tacttccttg atgtctccac cctctggccc     7200

actgcttacc cttggtaacg gcttggcttt tccttcttct ctccaggtct ggagcccgta     7260

gcccctctga cagtgtacgc tgctgaaggt tctagggtgg agctgccctg tcatttgccc     7320

ccaggagtgg ggaccccttc tttgctcatt gccaagtgga ctcctcctgg aggaggtcct     7380

gagctccccg tggctggaaa gagtggcaat tttacccttc accttgaggc tgtgggtctg     7440

gcacaggctg ggacctacac ctgtagcatc catctgcagg gacagcagct caatgccact     7500

gtcacgttgg cggtcatcac ag                                              7522


<210>  11
<211>  2292
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a humanized Lag3 allele after recombinase-mediated excision of a 
       selection cassette

<400>  11
ccatcacttt gtataagggc agatcccaaa gctgcctcag cctcccttca acagggaggc       60

atgatgtttc tttcttagga aagccagggc atttctctat tctccaatct cttggctcaa      120

tgcccttggc ctctcttttg ttccactagt gaagcctctc cagccagggg ctgaggtccc      180

ggtggtgtgg gcccaggagg gggctcctgc ccagctcccc tgcagcccca caatccccct      240

ccaggatctc agccttctgc gaagagcagg ggtcacttgg cagcatcagc cagacaggta      300

tgcaccccaa acttgggcaa caggacctcc gaatccagca ctcaacccca cacccgtgcc      360

ggtcctctgt cccctgccct gaggtgtcac tccctctgaa gccagtgacc cagtctccct      420

gccctcgctt gcaccgttcc tgcccttgct ctgcaatcag cgaccctcac gccagcatcc      480

cttctctcca gaagtggatg cggccagtcc aacagagggg tcgggcgtga ggggacggtt      540

ggtggtcaag agaactcttg gggcgggctt tctcatcctc aacgggtggc tgcctgcatc      600

ctcccgggct tcctacccct ggagcttctc aactccattc tctttcccgc ccagtggccc      660

gcccgctgcc gcccccggcc atcccctggc ccccggccct cacccggcgg cgccctcctc      720

ctgggggccc aggccccgcc gctacacggt gctgagcgtg ggtcccggag gcctgcgcag      780

cgggaggctg cccctgcagc cccgcgtcca gctggatgag cgcggccggc agcgcgggga      840

cttctcgcta tggctgcgcc cagcccggcg cgcggacgcc ggcgagtacc gcgccgcggt      900

gcacctcagg gaccgcgccc tctcctgccg cctccgtctg cgcctgggcc aggcctcgag      960

tatgtggggc gggacgatgg gagaagggct gggaggtggg tccccatccc ctgcctcccg     1020

ggacgcagga agggctgggg cagaggctgc gccctaggcc ctgtcggaga gctcccagaa     1080

gagtagagga agggggtggg cggcctgctg gagtggaagg tgcccccgaa gcacgtgtat     1140

ggggggccct gtggagagat tgtgtcaccc ccgagctccc cttctcccac ccacgcggga     1200

gtgcccagag ggagggggag ggggggagag catggggcta aagtgattca tttcagatat     1260

ctgtagctca gggggtgggc ttcgcggggt tccaggccag gaaaacggca agggtggctg     1320

atgccaagta aactccaggc cagggacggg gaaagtggtc ctggggagtc ttggggatcc     1380

actttatgca cctccaggtg ctggaagctg agatggggag agggtgatgt gggagaggag     1440

aagacaagtc taaagccagg tgcctgtttc caggagcttc cggcttggca gccctgctgt     1500

gttgggaaat tgtttccagt gggctgatga agtcttcttt atccttgcac agtgactgcc     1560

agccccccag gatctctcag agcctccgac tgggtcattt tgaactgctc cttcagccgc     1620

cctgaccgcc cagcctctgt gcattggttc cggaaccggg gccagggccg agtccctgtc     1680

cgggagtccc cccatcacca cttagcggaa agcttcctct tcctgcccca agtcagcccc     1740

atggactctg ggccctgggg ctgcatcctc acctacagag atggcttcaa cgtctccatc     1800

atgtataacc tcactgttct gggtaactcc cccactctgc ttcacatttg accacaactc     1860

cttcctgccc cccttgtcac ctcccctaac gtcgagataa cttcgtataa tgtatgctat     1920

acgaagttat gctagtaact ataacggtcc taaggtagcg agctagcgac ccccaaaact     1980

ttctcagctg cgtgtggtct cactccacat cactttgttt cagtgtccaa accattttct     2040

ctctgggcat cttttagctg ctgtctctct tacttttatt tatttatttg tgtgtttatt     2100

tatttatttt cattttagcg tgcgttggtg ttttgcctgc atagatgtct gtgtcagggt     2160

attggattcc ctggaacttg acctacagac agtcatgaga taccatatgg gtgctgggaa     2220

ttgaacccag ctcctctgga aggacagcca gtgttctaat ctgccatctc tcactgttta     2280

tcccttggct gt                                                         2292


<210>  12
<211>  160
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the nucleotide sequence across the upstream insertion point 
       between a mouse Lag-3 genomic sequence and a human LAG-3 genomic 
       sequence

<400>  12
catgatgttt ctttcttagg aaagccaggg catttctcta ttctccaatc tcttggctca       60

atgcccttgg cctctctttt gttccactag tgaagcctct ccagccaggg gctgaggtcc      120

cggtggtgtg ggcccaggag ggggctcctg cccagctccc                            160


<210>  13
<211>  190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the nucleotide sequence comprising a human LAG-3 genomic sequence
       contiguous with the 5' end of a self-deleting neomycin cassette

<400>  13
ttcacatttg accacaactc cttcctgccc cccttgtcac ctcccctaac gtcgagataa       60

cttcgtataa tgtatgctat acgaagttat atgcatggcc tccgcgccgg gttttggcgc      120

ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg      180

agcgtcctga                                                             190


<210>  14
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the nucleotide sequence across the downstream insertion point 
       comprising the 3' end of a self-deleting neomycin cassette 
       contiguous with a mouse Lag-3 genomic sequence

<400>  14
tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga       60

ataacttcgt ataatgtatg ctatacgaag ttatgctagt aactataacg gtcctaaggt      120

agcgagctag cgacccccaa aactttctca gctgcgtgtg gtctcactcc acatcacttt      180

gtttcagtgt ccaaaccatt ttctctctgg gcatctttta g                          221


<210>  15
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the nucleotide sequence across the upstream insertion point after
       deletion of a neomycin cassette

<400>  15
acgtctccat catgtataac ctcactgttc tgggtaactc ccccactctg cttcacattt       60

gaccacaact ccttcctgcc ccccttgtca cctcccctaa cgtcgagata acttcgtata      120

atgtatgcta tacgaagtta tgctagtaac tataacggtc ctaaggtagc gagctagcga      180

cccccaaaac tttctcagct gcgtgtggtc tcactccaca tcactttgtt tcagtgtcca      240

aaccattttc tctctgggca tcttttagct gctgtctc                              278


<210>  16
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  16
gaggctgctg acggtcaag                                                    19


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  17
ttaggcaggt taactttatc ctcaaagca                                         29


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  18
gccacgaaga agatgcactc aag                                               23


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  19
gtcccgggtc tcttggagat                                                   20


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  20
ccacccataa acatccccag gtttca                                            26


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  21
ccgctcattc caagtcagtt c                                                 21


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  22
cggttggtgg tcaagagaac                                                   20


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  23
cgggctttct catcctcaac ggg                                               23


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  24
ggcgggaaag agaatggagt tg                                                22


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  25
agccctgctg tgttgggaaa                                                   20


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  26
tgtttccagt gggctgatga agtc                                              24


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  27
tggcagtcac tgtgcaag                                                     18


