                         SEQUENCE LISTING

<110>  Washington University in Saint Louis
       Zhang, Fuzhong
       Jiang, Wen
       Bentley, Gayle
 
<120>  HOST CELLS AND METHODS FOR PRODUCING FATTY ACID-DERIVATIVES WITH 
       HIGH BRANCHED-CHAIN PERCENTAGE

<130>  WUSTL_015503ORD

<150>  US 62/268,191
<151>  2015-12-16

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  993
<212>  DNA
<213>  Bacillus subtilis

<400>  1
atgagtacaa accgacatca agcactaggg ctgactgatc aggaagccgt tgatatgtat       60

agaaccatgc tgttagcaag aaaaatcgat gaaagaatgt ggctgttaaa ccgttctggc      120

aaaattccat ttgtaatctc ttgtcaagga caggaagcag cacaggtagg agcggctttc      180

gcacttgacc gtgaaatgga ttatgtattg ccgtactaca gagacatggg tgtcgtgctc      240

gcgtttggca tgacagcaaa ggacttaatg atgtccgggt ttgcaaaagc agcagatccg      300

aactcaggag gccgccagat gccgggacat ttcggacaaa agaaaaaccg cattgtgacg      360

ggatcatctc cggttacaac gcaagtgccg cacgcagtcg gtattgcgct tgcgggacgt      420

atggagaaaa aggatatcgc agcctttgtt acattcgggg aagggtcttc aaaccaaggc      480

gatttccatg aaggggcaaa ctttgccgct gtccataagc tgccggttat tttcatgtgt      540

gaaaacaaca aatacgcaat ctcagtgcct tacgataagc aagtcgcatg tgagaacatt      600

tccgaccgtg ccataggcta tgggatgcct ggcgtaactg tgaatggaaa tgatccgctg      660

gaagtttatc aagcggttaa agaagcacgc gaaagggcac gcagaggaga aggcccgaca      720

ttaattgaaa cgatttctta ccgccttaca ccacattcca gtgatgacga tgacagcagc      780

tacagaggcc gtgaagaagt agaggaagcg aaaaaaagtg atcccctgct tacttatcaa      840

gcttacttaa aggaaacagg cctgctgtcc gatgagatag aacaaaccat gctggatgaa      900

attatggcaa tcgtaaatga agcgacggat gaagcggaga acgccccata tgcagctcct      960

gagtcagcgc ttgattatgt ttatgcgaag tag                                   993


<210>  2
<211>  984
<212>  DNA
<213>  Bacillus subtilis

<400>  2
atgtcagtaa tgtcatatat tgatgcaatc aatttggcga tgaaagaaga aatggaacga       60

gattctcgcg ttttcgtcct tggggaagat gtaggaagaa aaggcggtgt gtttaaagcg      120

acagcgggac tctatgaaca atttggggaa gagcgcgtta tggatacgcc gcttgctgaa      180

tctgcaatcg caggagtcgg tatcggagcg gcaatgtacg gaatgagacc gattgctgaa      240

atgcagtttg ctgatttcat tatgccggca gtcaaccaaa ttatttctga agcggctaaa      300

atccgctacc gcagcaacaa tgactggagc tgtccgattg tcgtcagagc gccatacggc      360

ggaggcgtgc acggagccct gtatcattct caatcagtcg aagcaatttt cgccaaccag      420

cccggactga aaattgtcat gccatcaaca ccatatgacg cgaaagggct cttaaaagcc      480

gcagttcgtg acgaagaccc cgtgctgttt tttgagcaca agcgggcata ccgtctgata      540

aagggcgagg ttccggctga tgattatgtc ctgccaatcg gcaaggcgga cgtaaaaagg      600

gaaggcgacg acatcacagt gatcacatac ggcctgtgtg tccacttcgc cttacaagct      660

gcagaacgtc tcgaaaaaga tggcatttca gcgcatgtgg tggatttaag aacagtttac      720

ccgcttgata aagaagccat catcgaagct gcgtccaaaa ctggaaaggt tcttttggtc      780

acagaagata caaaagaagg cagcatcatg agcgaagtag ccgcaattat atccgagcat      840

tgtctgttcg acttagacgc gccgatcaaa cggcttgcag gtcctgatat tccggctatg      900

ccttatgcgc cgacaatgga aaaatacttt atggtcaacc ctgataaagt ggaagcggcg      960

atgagagaat tagcggagtt ttaa                                             984


<210>  3
<211>  1275
<212>  DNA
<213>  Bacillus subtilis

<400>  3
atggcaattg aacaaatgac gatgccgcag cttggagaaa gcgtaacaga ggggacgatc       60

agcaaatggc ttgtcgcccc cggtgataaa gtgaacaaat acgatccgat cgcggaagtc      120

atgacagata aggtaaatgc agaggttccg tcttctttta ctggtacgat aacagagctt      180

gtgggagaag aaggccaaac cctgcaagtc ggagaaatga tttgcaaaat tgaaacagaa      240

ggcgcgaatc cggctgaaca aaaacaagaa cagccagcag catcagaagc cgctgagaac      300

cctgttgcaa aaagtgctgg agcagccgat cagcccaata aaaagcgcta ctcgccagct      360

gttctccgtt tggccggaga gcacggcatt gacctcgatc aagtgacagg aactggtgcc      420

ggcgggcgca tcacacgaaa agatattcag cgcttaattg aaacaggcgg cgtgcaagaa      480

cagaatcctg aggagctgaa aacagcagct cctgcaccga agtctgcatc aaaacctgag      540

ccaaaagaag agacgtcata tcctgcgtct gcagccggtg ataaagaaat ccctgtcaca      600

ggtgtaagaa aagcaattgc ttccaatatg aagcgaagca aaacagaaat tccgcatgct      660

tggacgatga tggaagtcga cgtcacaaat atggttgcat atcgcaacag tataaaagat      720

tcttttaaga agacagaagg ctttaattta acgttcttcg ccttttttgt aaaagcggtc      780

gctcaggcgt taaaagaatt cccgcaaatg aatagcatgt gggcggggga caaaattatt      840

cagaaaaagg atatcaatat ttcaattgca gttgccacag aggattcttt atttgttccg      900

gtgattaaaa acgctgatga aaaaacaatt aaaggcattg cgaaagacat taccggccta      960

gctaaaaaag taagagacgg aaaactcact gcagatgaca tgcagggagg cacgtttacc     1020

gtcaacaaca caggttcgtt cgggtctgtt cagtcgatgg gcattatcaa ctaccctcag     1080

gctgcgattc ttcaagtaga atccatcgtc aaacgcccgg ttgtcatgga caatggcatg     1140

attgctgtca gagacatggt taatctgtgc ctgtcattag atcacagagt gcttgacggt     1200

ctcgtgtgcg gacgattcct cggacgagtg aaacaaattt tagaatcgat tgacgagaag     1260

acatctgttt actaa                                                      1275


<210>  4
<211>  1425
<212>  DNA
<213>  Bacillus subtilis

<400>  4
atggcaactg agtatgacgt agtcattctg ggcggcggta ccggcggtta tgttgcggcc       60

atcagagccg ctcagctcgg cttaaaaaca gccgttgtgg aaaaggaaaa actcggggga      120

acatgtctgc ataaaggctg tatcccgagt aaagcgctgc ttagaagcgc agaggtatac      180

cggacagctc gtgaagccga tcaattcgga gtggaaacgg ctggcgtgtc cctcaacttt      240

gaaaaagtgc agcagcgtaa gcaagccgtt gttgataagc ttgcagcggg tgtaaatcat      300

ttaatgaaaa aaggaaaaat tgacgtgtac accggatatg gacgtatcct tggaccgtca      360

atcttctctc cgctgccggg aacaatttct gttgagcggg gaaatggcga agaaaatgac      420

atgctgatcc cgaaacaagt gatcattgca acaggatcaa gaccgagaat gcttccgggt      480

cttgaagtgg acggtaagtc tgtactgact tcagatgagg cgctccaaat ggaggagctg      540

ccacagtcaa tcatcattgt cggcggaggg gttatcggta tcgaatgggc gtctatgctt      600

catgattttg gcgttaaggt aacggttatt gaatacgcgg atcgcatatt gccgactgaa      660

gatctagaga tttcaaaaga aatggaaagt cttcttaaga aaaaaggcat ccagttcata      720

acaggggcaa aagtgctgcc tgacacaatg acaaaaacat cagacgatat cagcatacaa      780

gcggaaaaag acggagaaac cgttacctat tctgctgaga aaatgcttgt ttccatcggc      840

agacaggcaa atatcgaagg catcggccta gagaacaccg atattgttac tgaaaatggc      900

atgatttcag tcaatgaaag ctgccaaacg aaggaatctc atatttatgc aatcggagac      960

gtaatcggtg gcctgcagtt agctcacgtt gcttcacatg agggaattat tgctgttgag     1020

cattttgcag gtctcaatcc gcatccgctt gatccgacgc ttgtgccgaa gtgcatttac     1080

tcaagccctg aagctgccag tgtcggctta accgaagacg aagcaaaggc gaacgggcat     1140

aatgtcaaaa tcggcaagtt cccatttatg gcgattggaa aagcgcttgt atacggtgaa     1200

agcgacggtt ttgtcaaaat cgtggctgac cgagatacag atgatattct cggcgttcat     1260

atgattggcc cgcatgtcac cgacatgatt tctgaagcgg gtcttgccaa agtgctggac     1320

gcaacaccgt gggaggtcgg gcaaacgatt cacccgcatc caacgctttc tgaagcaatt     1380

ggagaagctg cgcttgccgc agatggcaaa gccattcatt tttaa                     1425


<210>  5
<211>  942
<212>  DNA
<213>  Staphylococcus aureus

<400>  5
atgaacgtgg gtattaaagg ttttggtgca tatgcgccag aaaagattat tgacaatgcc       60

tattttgagc aatttttaga tacatctgat gaatggattt ctaagatgac tggaattaaa      120

gaaagacatt gggcagatga tgatcaagat acttcagatt tagcatatga agcaagttta      180

aaagcaatcg ctgacgctgg tattcagccc gaagatatag atatgataat tgttgccaca      240

gcaactggag atatgccatt tccaactgtc gcaaatatgt tgcaagaacg tttagggacg      300

ggcaaagttg cctctatgga tcaacttgca gcatgttctg gatttatgta ttcaatgatt      360

acagctaaac aatatgttca atctggagat tatcataaca ttttagttgt cggtgcagat      420

aaattatcta aaataacaga tttaactgac cgttctactg cagttctatt tggagatggt      480

gcaggtgcgg ttatcatcgg tgaagtttca gatggcagag gtattataag ttatgaaatg      540

ggttctgatg gcacaggtgg taaacattta tatttagata aagatactgg taaactgaaa      600

atgaatggtc gagaagtatt taaatttgct gttagaatta tgggtgatgc atcaacacgt      660

gtagttgaaa aagcgaattt aacatcagat gatatagatt tatttattcc tcatcaagct      720

aatattagaa ttatggaatc agctagagaa cgcttaggta tttcaaaaga caaaatgagt      780

gtttctgtaa ataaatatgg aaatacttca gctgcgtcaa tacctttaag tatcgatcaa      840

gaattaaaaa atggtaaaat caaagatgat gatacaattg ttcttgtcgg attcggtggc      900

ggcctaactt ggggcgcaat gacaataaaa tggggaaaat ag                         942


<210>  6
<211>  978
<212>  DNA
<213>  Bacillus subtilis

<400>  6
atgtcaaaag caaaaattac agctatcggc acctatgcgc cgagcagacg tttaaccaat       60

gcagatttag aaaagatcgt tgatacctct gatgaatgga tcgttcagcg cacaggaatg      120

agagaacgcc ggattgcgga tgaacatcaa tttacctctg atttatgcat agaagcggtg      180

aagaatctca agagccgtta taaaggaacg cttgatgatg tcgatatgat cctcgttgcc      240

acaaccacat ccgattacgc ctttccgagt acggcatgcc gcgtacagga atatttcggc      300

tgggaaagca ccggcgcgct ggatattaat gcgacatgcg ccgggctgac atacggcctc      360

catttggcaa atggattgat cacatctggc cttcatcaaa aaattctcgt catcgccgga      420

gagacgttat caaaggtaac cgattatacc gatcgaacga catgcgtact gttcggcgat      480

gccgcgggtg cgctgttagt agaacgagat gaagagacgc cgggatttct tgcgtctgta      540

caaggaacaa gcgggaacgg cggcgatatt ttgtatcgtg ccggactgcg aaatgaaata      600

aacggtgtgc agcttgtcgg ttccggaaaa atggtgcaaa acggacgcga ggtatataaa      660

tgggccgcaa gaaccgtccc tggcgaattt gaacggcttt tacataaagc aggactcagc      720

tccgatgatc tcgattggtt tgttcctcac agcgccaact tgcgcatgat cgagtcaatt      780

tgtgaaaaaa caccgttccc gattgaaaaa acgctcacta gtgttgagca ctacggaaac      840

acgtcttcgg tttcaattgt tttggcgctc gatctcgcag tgaaagccgg gaagctgaaa      900

aaagatcaaa tcgttttgct tttcgggttt ggcggcggat taacctatac aggattgctt      960

attaaatggg ggatgtaa                                                    978


<210>  7
<211>  552
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
atggcggaca cgttattgat tctgggtgat agcctgagcg ccgggtatcg aatgtctgcc       60

agcgcggcct ggcctgcctt gttgaatgat aagtggcaga gtaaaacgtc ggtagttaat      120

gccagcatca gcggcgacac ctcgcaacaa ggactggcgc gccttccggc tctgctgaaa      180

cagcatcagc cgcgttgggt gctggttgaa ctgggcggca atgacggttt gcgtggtttt      240

cagccacagc aaaccgagca aacgctgcgc cagattttgc aggatgtcaa agccgccaac      300

gctgaaccat tgttaatgca aatacgtctg cctgcaaact atggtcgccg ttataatgaa      360

gcctttagcg ccatttaccc caaactcgcc aaagagtttg atgttccgct gctgcccttt      420

tttatggaag aggtctacct caagccacaa tggatgcagg atgacggtat tcatcccaac      480

cgcgacgccc agccgtttat tgccgactgg atggcgaagc agttgcagcc tttagtaaat      540

catgactcat aa                                                          552


<210>  8
<211>  1017
<212>  DNA
<213>  Escherichia coli

<400>  8
atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg       60

gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat      120

gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg      180

atggaagaag ataacgtccg cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac      240

gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaactatc      300

tccacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga      360

cgtaacgatc tggtggtgaa aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat      420

cgcgaaacca aagatcgcgg cttccaccac ggcaccttgc tactcaatgc cgacctcagc      480

cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacgtcg      540

gtacgttccc gcgtgaccaa cctcaccgag ctgttgccgg ggatcaccca tgagcaggtt      600

tgcgaggcca taaccgaggc ctttttcgcc cattatggcg agcgcgtgga agcggaaatc      660

atctccccga acaaaacgcc agacttgcca aacttcgccg aaacctttgc ccgccagagt      720

agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt      780

acctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag      840

gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccggacg actgcaaggc      900

tgcctgtacc gcgcagatat gctgcaacag gagtgcgaag cgctgttggt tgacttcccg      960

gaacaggaaa aagagctacg ggagttatcg gcatggatgg cgggggctgt aaggtag        1017


<210>  9
<211>  720
<212>  DNA
<213>  Escherichia coli

<400>  9
atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc       60

tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta      120

attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg      180

ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta      240

aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat      300

ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat      360

cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc      420

tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt      480

tacggtctga ttcttaacgg gatgaaaggg ctgtatacgc gtattggtcg tcactatttc      540

gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc      600

agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc      660

gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa      720


<210>  10
<211>  642
<212>  DNA
<213>  Escherichia coli

<400>  10
ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc       60

caggctatgc atgaattcac cgatacccgc gatgatagta cccttgatga aatctggctg      120

gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca cattttaatg      180

ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg      240

gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa      300

ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg      360

catcctcggg ctgacgcgcc aggtgtctat gttggggaaa agaaaatttg ctcactgggt      420

ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt      480

tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc taaaatatca      540

caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga aaatatttta      600

gcgctactaa acaatccgga cttcgaatat attaccgctt aa                         642


<210>  11
<211>  966
<212>  DNA
<213>  Escherichia coli

<400>  11
atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc       60

cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg      120

atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc      180

aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc      240

aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg ttgtccgttc      300

tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt gaaactggcg      360

cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac      420

ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc      480

ccgcaaatca aaattgaaac tctggtgccg gatttccgcg gtcgtatgga tcgtgctctg      540

gatattctga ctgcaacgcc accagatgtg ttcaaccata acctggaaaa cgtaccgcgt      600

atttaccgtc aggtacggcc tggtgcagat tacaactggt cgctgaagct gctggaacgc      660

tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg actgggtgaa      720

accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta      780

acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc      840

ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca      900

tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt      960

aagtaa                                                                 966


