                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  Methods and Compositions for Treatment of Ocular Disorders and 
       Blinding Diseases

<130>  UPN-15-7572PCT

<150>  62/275006
<151>  2016-01-05

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  665
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (23)..(655)

<400>  1
ggccatacag gccgccacca tg gcc tca ctg ttc tcc ggg cgc atc ctc atc        52
                         Ala Ser Leu Phe Ser Gly Arg Ile Leu Ile          
                         1               5                   10           

cga aac aac agc gat cag gac gaa ttg gac acc gag gct gaa gtc tcc        100
Arg Asn Asn Ser Asp Gln Asp Glu Leu Asp Thr Glu Ala Glu Val Ser           
                15                  20                  25                

cgc cgg ctg gaa aac agg ctc gtg ctc ctg ttc ttc ggt gcc gga gcg        148
Arg Arg Leu Glu Asn Arg Leu Val Leu Leu Phe Phe Gly Ala Gly Ala           
            30                  35                  40                    

tgc ccg cag tgc caa gcc ttc gtc cca att ctt aag gac ttc ttt gtg        196
Cys Pro Gln Cys Gln Ala Phe Val Pro Ile Leu Lys Asp Phe Phe Val           
        45                  50                  55                        

cgc ctc act gat gag ttt tac gtg ctc cgg gca gcg cag ctg gcc ttg        244
Arg Leu Thr Asp Glu Phe Tyr Val Leu Arg Ala Ala Gln Leu Ala Leu           
    60                  65                  70                            

gtg tat gtg tcg caa gat tcc act gag gaa caa cag gac ctg ttc ctg        292
Val Tyr Val Ser Gln Asp Ser Thr Glu Glu Gln Gln Asp Leu Phe Leu           
75                  80                  85                  90            

aaa gac atg cct aag aag tgg ctt ttc ctg ccc ttc gag gac gac ctg        340
Lys Asp Met Pro Lys Lys Trp Leu Phe Leu Pro Phe Glu Asp Asp Leu           
                95                  100                 105               

aga agg gac ctg gga cgc cag ttc agc gtg gaa cgg ctg ccg gcc gtc        388
Arg Arg Asp Leu Gly Arg Gln Phe Ser Val Glu Arg Leu Pro Ala Val           
            110                 115                 120                   

gtg gtg ctg aag ccc gac ggg gac gtg ctt acc cgg gat ggc gct gac        436
Val Val Leu Lys Pro Asp Gly Asp Val Leu Thr Arg Asp Gly Ala Asp           
        125                 130                 135                       

gaa atc cag agg ctg ggc acc gcc tgt ttc gca aat tgg cag gag gcc        484
Glu Ile Gln Arg Leu Gly Thr Ala Cys Phe Ala Asn Trp Gln Glu Ala           
    140                 145                 150                           

gcc gaa gtg ctc gac cgg aac ttc cag ctg ccc gag gat ctg gag gac        532
Ala Glu Val Leu Asp Arg Asn Phe Gln Leu Pro Glu Asp Leu Glu Asp           
155                 160                 165                 170           

cag gaa cct cgg tcc ctg acc gag tgc ctc aga cgc cac aag tac cgc        580
Gln Glu Pro Arg Ser Leu Thr Glu Cys Leu Arg Arg His Lys Tyr Arg           
                175                 180                 185               

gtg gaa aag gcc gcg aga gga gga cgg gac ccg ggt ggc ggg gga ggc        628
Val Glu Lys Ala Ala Arg Gly Gly Arg Asp Pro Gly Gly Gly Gly Gly           
            190                 195                 200                   

gaa gag ggc gga gcc ggt ggc ctg ttc tgatagatct                         665
Glu Glu Gly Gly Ala Gly Gly Leu Phe                                       
        205                 210                                           


<210>  2
<211>  348
<212>  DNA
<213>  Homo sapiens

<400>  2
gcggccgcca ccatggccag cctcttctcc ggacgcatcc tgattcgcaa caattccgac       60

caagacgaac tggataccga ggccgaagtc tcgcggagat tggagaacag gcttgtgctg      120

ctgttctttg gcgcgggagc gtgtcctcag tgccaggctt tcgtgccaat cctgaaggat      180

ttcttcgtgc ggctgactga cgaattctac gtcctccggg ccgcccagct ggcactggtg      240

tacgtgtccc aagactcaac cgaggaacag caggatctgt tcctcaagga catgcccaaa      300

aagtggctgt tcctgccgtt tgaggacgac ttgcggcgct agtgatca                   348


<210>  3
<211>  636
<212>  DNA
<213>  Homo sapiens

<400>  3
atggcctccc tgttctctgg ccgcatcctg atccgcaaca atagcgacca ggacgagctg       60

gatacggagg ctgaggtcag tcgcaggctg gagaaccggc tggtgctgct gttctttggt      120

gctggggctt gtccacagtg ccaggccttc gtgcccatcc tcaaggactt cttcgtgcgg      180

ctcacagatg agttctatgt actgcgggcg gctcagctgg ccctggtgta cgtgtcccag      240

gactccacgg aggagcagca ggacctgttc ctcaaggaca tgccaaagaa atggcttttc      300

ctgccctttg aggatgatct gaggagggac ctcgggcgcc agttctcagt ggagcgcctg      360

ccggcggtcg tggtgctcaa gccggacggg gacgtgctca ctcgcgacgg cgccgacgag      420

atccagcgcc tgggcaccgc ctgcttcgcc aactggcagg aggcggccga ggtgctggac      480

cgcaacttcc agctgccaga ggacctggag gaccaggagc cacggagcct caccgagtgc      540

ctgcgccgcc acaagtaccg cgtggaaaag gcggcgcgag gcgggcgcga ccccggggga      600

gggggtgggg aggagggcgg ggccgggggg ctgttc                                636


<210>  4
<211>  4837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rAAV genome with ITRs flanking an expression cassette containing 
       RdCVF sequences and promoters


<220>
<221>  misc_feature
<222>  (17)..(146)
<223>  AAV 5' ITR

<220>
<221>  promoter
<222>  (207)..(1478)

<220>
<221>  gene
<222>  (1479)..(1826)
<223>  hRdCVF short form codon optimized sequence

<220>
<221>  polyA_site
<222>  (1821)..(2047)

<220>
<221>  terminator
<222>  (2042)..(2479)

<220>
<221>  promoter
<222>  (2474)..(3774)

<220>
<221>  gene
<222>  (3762)..(4426)
<223>  hRdCVFL long form codon optimized

<220>
<221>  polyA_signal
<222>  (4427)..(4643)

<220>
<221>  misc_feature
<222>  (4691)..(4820)
<223>  AAV 3' ITR

<400>  4
gtcgacttaa ttaaggctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg       60

gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt      120

ggccaactcc atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc      180

tacgtagcaa gctagctagt tattaatagt aatcaattac ggggtcatta gttcatagcc      240

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      300

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      360

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      420

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      480

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      540

tagtcatcgc tattaacatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct      600

cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga      660

tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg      720

gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc      780

cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg      840

ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc      900

gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggatg agcacggccc      960

ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg     1020

gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg     1080

ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca     1140

ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc     1200

ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg     1260

cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc     1320

ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg     1380

gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagacaat tgtactaacc     1440

ttcttctctt tcctctcctg acaggttggt gtacactagc ggccgccacc atggccagcc     1500

tcttctccgg acgcatcctg attcgcaaca attccgacca agacgaactg gataccgagg     1560

ccgaagtctc gcggagattg gagaacaggc ttgtgctgct gttctttggc gcgggagcgt     1620

gtcctcagtg ccaggctttc gtgccaatcc tgaaggattt cttcgtgcgg ctgactgacg     1680

aattctacgt cctccgggcc gcccagctgg cactggtgta cgtgtcccaa gactcaaccg     1740

aggaacagca ggatctgttc ctcaaggaca tgcccaaaaa gtggctgttc ctgccgtttg     1800

aggacgactt gcggcgctag tgatcagcct cgactgtgcc ttctagttgc cagccatctg     1860

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt     1920

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg     1980

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg     2040

aggatccaat ttattctaaa tgcataataa atactgataa catcttatag tttgtattat     2100

attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt ccctttatta     2160

ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta tatacaaaaa     2220

atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa aagcaacgta     2280

tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc tcatatatca     2340

agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa actcccccca     2400

taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt actcgttatc     2460

agaaccgccc aggaagcttt agttattaat agtaatcaat tacggggtca ttagttcata     2520

gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc     2580

ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag     2640

ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac     2700

atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg     2760

cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg     2820

tattagtcat cgctattaac atggtcgagg tgagccccac gttctgcttc actctcccca     2880

tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag     2940

cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc     3000

ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt     3060

ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg     3120

cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg     3180

cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg atgagcacgg     3240

cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg     3300

ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc     3360

gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag     3420

ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg     3480

tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg     3540

gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg     3600

tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg     3660

acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagac aattgtacta     3720

accttcttct ctttcctctc ctgacaggtt ggtgtacact aggccataca ggccgccacc     3780

atggcctcac tgttctccgg gcgcatcctc atccgaaaca acagcgatca ggacgaattg     3840

gacaccgagg ctgaagtctc ccgccggctg gaaaacaggc tcgtgctcct gttcttcggt     3900

gccggagcgt gcccgcagtg ccaagccttc gtcccaattc ttaaggactt ctttgtgcgc     3960

ctcactgatg agttttacgt gctccgggca gcgcagctgg ccttggtgta tgtgtcgcaa     4020

gattccactg aggaacaaca ggacctgttc ctgaaagaca tgcctaagaa gtggcttttc     4080

ctgcccttcg aggacgacct gagaagggac ctgggacgcc agttcagcgt ggaacggctg     4140

ccggccgtcg tggtgctgaa gcccgacggg gacgtgctta cccgggatgg cgctgacgaa     4200

atccagaggc tgggcaccgc ctgtttcgca aattggcagg aggccgccga agtgctcgac     4260

cggaacttcc agctgcccga ggatctggag gaccaggaac ctcggtccct gaccgagtgc     4320

ctcagacgcc acaagtaccg cgtggaaaag gccgcgagag gaggacggga cccgggtggc     4380

gggggaggcg aagagggcgg agccggtggc ctgttctgat agatctgcct cgactgtgcc     4440

ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     4500

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     4560

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     4620

caatagcagg catgctgggg actcgagttc tacgtagata agtagcatgg cgggttaatc     4680

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     4740

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     4800

gtgagcgagc gagcgcgcag ccttaattaa cctaagg                              4837


<210>  5
<211>  4745
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rAAV genome with copies of native hRdCVF and promoters


<220>
<221>  misc_feature
<222>  (9)..(100)
<223>  KanR complement

<220>
<221>  misc_feature
<222>  (117)..(246)
<223>  AAV 5' ITR

<220>
<221>  promoter
<222>  (307)..(1578)

<220>
<221>  gene
<222>  (1591)..(1935)
<223>  human RdCVF native short form

<220>
<221>  polyA_signal
<222>  (1940)..(2161)

<220>
<221>  terminator
<222>  (2246)..(2546)

<220>
<221>  misc_feature
<222>  (2337)..(2362)
<223>  pTF3 complement

<220>
<221>  terminator
<222>  (2553)..(2666)

<220>
<221>  promoter
<222>  (2697)..(3968)

<220>
<221>  gene
<222>  (3981)..(4325)
<223>  hRDCVF native short form second copy

<220>
<221>  polyA_signal
<222>  (4330)..(4551)

<220>
<221>  misc_feature
<222>  (4599)..(4728)
<223>  AAV 3' ITR

<400>  5
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa gtcgacttaa ttaaggctgc      120

gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc      180

gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg      240

gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcaa gctagctagt      300

tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt      360

acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      420

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      480

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      540

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      600

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaacatg      660

gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca      720

attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg      780

gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt      840

gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg      900

cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc      960

ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg     1020

cgttactccc acaggtgagc gggcgggatg agcacggccc ggcttcgggt gcggggctcc     1080

gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc     1140

cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc     1200

ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt     1260

gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc     1320

gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa     1380

tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc     1440

ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct     1500

tctggcgtgt gaccggcggc tctagacaat tgtactaacc ttcttctctt tcctctcctg     1560

acaggttggt gtacactagc ggccgccacc atggcctccc tgttctctgg ccgcatcctg     1620

atccgcaaca atagcgacca ggacgagctg gatacggagg ctgaggtcag tcgcaggctg     1680

gagaaccggc tggtgctgct gttctttggt gctggggctt gtccacagtg ccaggccttc     1740

gtgcccatcc tcaaggactt cttcgtgcgg ctcacagatg agttctatgt actgcgggcg     1800

gctcagctgg ccctggtgta cgtgtcccag gactccacgg aggagcagca ggacctgttc     1860

ctcaaggaca tgccaaagaa atggcttttc ctgccctttg aggatgatct gaggaggtga     1920

tcatctcatg gatccaagag atctgcctcg actgtgcctt ctagttgcca gccatctgtt     1980

gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc     2040

taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt     2100

ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac     2160

tcgataagga aaatgaagtg aagttcctat actttctaga gaataggaac ttctatagtg     2220

agtcgaataa gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt     2280

atagtttgta ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga     2340

ttttcccttt attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat     2400

tgtatataca aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc     2460

gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata     2520

attctcatat atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc     2580

ctaaactccc cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac     2640

gattactcgt tatcagaacc gcccaggggg cccgagctta actagctagt tattaatagt     2700

aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta     2760

cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga     2820

cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt     2880

tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta     2940

ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg     3000

actttcctac ttggcagtac atctacgtat tagtcatcgc tattaacatg gtcgaggtga     3060

gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt     3120

tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc     3180

caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag     3240

ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc     3300

cctataaaaa gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc ttcgccccgt     3360

gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc     3420

acaggtgagc gggcgggatg agcacggccc ggcttcgggt gcggggctcc gtacggggcg     3480

tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc     3540

ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg     3600

cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc     3660

gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac     3720

cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga     3780

gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc     3840

gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt     3900

gaccggcggc tctagacaat tgtactaacc ttcttctctt tcctctcctg acaggttggt     3960

gtacactagc ggccgccacc atggcctccc tgttctctgg ccgcatcctg atccgcaaca     4020

atagcgacca ggacgagctg gatacggagg ctgaggtcag tcgcaggctg gagaaccggc     4080

tggtgctgct gttctttggt gctggggctt gtccacagtg ccaggccttc gtgcccatcc     4140

tcaaggactt cttcgtgcgg ctcacagatg agttctatgt actgcgggcg gctcagctgg     4200

ccctggtgta cgtgtcccag gactccacgg aggagcagca ggacctgttc ctcaaggaca     4260

tgccaaagaa atggcttttc ctgccctttg aggatgatct gaggaggtga tcatctcatg     4320

gatccaagag atctgcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct     4380

cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg     4440

aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc     4500

aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac tcgagttcta     4560

cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt     4620

ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg     4680

acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc     4740

taagg                                                                 4745


<210>  6
<211>  12464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing the p857 rAAV genome containing native 2xRdCVF


<220>
<221>  misc_feature
<222>  (9)..(903)
<223>  KanR complement

<220>
<221>  misc_feature
<222>  (988)..(1162)
<223>  B1 B2 T1 Txn Terminator Complement

<220>
<221>  misc_feature
<222>  (1063)..(1079)
<223>  pTR

<220>
<221>  misc_feature
<222>  (1253)..(1382  )
<223>  AAV 5  ITR

<220>
<221>  promoter
<222>  (1443)..(2714)

<220>
<221>  gene
<222>  (2727)..(3071)
<223>  hRdCVF (native short form)

<220>
<221>  polyA_signal
<222>  (3076)..(3297)

<220>
<221>  terminator
<222>  (3382)..(3682)

<220>
<221>  misc_feature
<222>  (3473)..(3498)
<223>  pTF3 complement

<220>
<221>  terminator
<222>  (3689)..(3802)

<220>
<221>  promoter
<222>  (3833)..(5104)

<220>
<221>  gene
<222>  (5117)..(5461)
<223>  hRdCVF native short form second copy

<220>
<221>  polyA_signal
<222>  (5466)..(5687)

<220>
<221>  misc_feature
<222>  (5735)..(5864)
<223>  AAV 3' ITR

<220>
<221>  terminator
<222>  (5958)..(6258)

<220>
<221>  misc_feature
<222>  (6049)..(6074)
<223>  pTF3 Complement

<220>
<221>  terminator
<222>  (6265)..(6378)

<220>
<221>  misc_feature
<222>  (6394)..(11460)
<223>  Lambda Stuffer

<220>
<221>  misc_feature
<222>  (11621)..(12424)
<223>  pUC Ori Complement

<400>  6
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggtcg acttaattaa ggctgcgcgc     1260

tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc     1320

ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc     1380

cttgtagtta atgattaacc cgccatgcta cttatctacg tagcaagcta gctagttatt     1440

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat     1500

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa     1560

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg     1620

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc     1680

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct     1740

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt aacatggtcg     1800

aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt     1860

tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc     1920

gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg     1980

cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc     2040

ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg     2100

ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt     2160

actcccacag gtgagcgggc gggatgagca cggcccggct tcgggtgcgg ggctccgtac     2220

ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg     2280

cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag     2340

cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga     2400

gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg     2460

ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg     2520

cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg     2580

ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg     2640

gcgtgtgacc ggcggctcta gacaattgta ctaaccttct tctctttcct ctcctgacag     2700

gttggtgtac actagcggcc gccaccatgg cctccctgtt ctctggccgc atcctgatcc     2760

gcaacaatag cgaccaggac gagctggata cggaggctga ggtcagtcgc aggctggaga     2820

accggctggt gctgctgttc tttggtgctg gggcttgtcc acagtgccag gccttcgtgc     2880

ccatcctcaa ggacttcttc gtgcggctca cagatgagtt ctatgtactg cgggcggctc     2940

agctggccct ggtgtacgtg tcccaggact ccacggagga gcagcaggac ctgttcctca     3000

aggacatgcc aaagaaatgg cttttcctgc cctttgagga tgatctgagg aggtgatcat     3060

ctcatggatc caagagatct gcctcgactg tgccttctag ttgccagcca tctgttgttt     3120

gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat     3180

aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg     3240

tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggactcga     3300

taaggaaaat gaagtgaagt tcctatactt tctagagaat aggaacttct atagtgagtc     3360

gaataagggc gacacaaaat ttattctaaa tgcataataa atactgataa catcttatag     3420

tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa aactgatttt     3480

ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag aaatattgta     3540

tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca tcaatcgaaa     3600

aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt taaataattc     3660

tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct ctttccctaa     3720

actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg attaacgatt     3780

actcgttatc agaaccgccc agggggcccg agcttaacta gctagttatt aatagtaatc     3840

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt     3900

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta     3960

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg     4020

gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga     4080

cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt     4140

tcctacttgg cagtacatct acgtattagt catcgctatt aacatggtcg aggtgagccc     4200

cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt     4260

tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg     4320

cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa     4380

tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta     4440

taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg ccccgtgccc     4500

cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag     4560

gtgagcgggc gggatgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc     4620

gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg     4680

ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc     4740

tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag     4800

ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc     4860

tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc     4920

cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg     4980

ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc     5040

ggcggctcta gacaattgta ctaaccttct tctctttcct ctcctgacag gttggtgtac     5100

actagcggcc gccaccatgg cctccctgtt ctctggccgc atcctgatcc gcaacaatag     5160

cgaccaggac gagctggata cggaggctga ggtcagtcgc aggctggaga accggctggt     5220

gctgctgttc tttggtgctg gggcttgtcc acagtgccag gccttcgtgc ccatcctcaa     5280

ggacttcttc gtgcggctca cagatgagtt ctatgtactg cgggcggctc agctggccct     5340

ggtgtacgtg tcccaggact ccacggagga gcagcaggac ctgttcctca aggacatgcc     5400

aaagaaatgg cttttcctgc cctttgagga tgatctgagg aggtgatcat ctcatggatc     5460

caagagatct gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc     5520

cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga     5580

aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga     5640

cagcaagggg gaggattggg aagacaatag caggcatgct ggggactcga gttctacgta     5700

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc     5760

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc     5820

ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaag     5880

gaaaatgaag tgaagttcct atactttcta gagaatagga acttctatag tgagtcgaat     5940

aagggcgaca caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg     6000

tattatattt tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct     6060

ttattatttt cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata     6120

caaaaaatca taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc     6180

aacgtatctt atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat     6240

atatcaagca aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc     6300

cccccataaa aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc     6360

gttatcagaa ccgcccaggg ggcccgagct taaccttttt atttggggga gagggaagtc     6420

atgaaaaaac taacctttga aattcgatct ccagcacatc agcaaaacgc tattcacgca     6480

gtacagcaaa tccttccaga cccaaccaaa ccaatcgtag taaccattca ggaacgcaac     6540

cgcagcttag accaaaacag gaagctatgg gcctgcttag gtgacgtctc tcgtcaggtt     6600

gaatggcatg gtcgctggct ggatgcagaa agctggaagt gtgtgtttac cgcagcatta     6660

aagcagcagg atgttgttcc taaccttgcc gggaatggct ttgtggtaat aggccagtca     6720

accagcagga tgcgtgtagg cgaatttgcg gagctattag agcttataca ggcattcggt     6780

acagagcgtg gcgttaagtg gtcagacgaa gcgagactgg ctctggagtg gaaagcgaga     6840

tggggagaca gggctgcatg ataaatgtcg ttagtttctc cggtggcagg acgtcagcat     6900

atttgctctg gctaatggag caaaagcgac gggcaggtaa agacgtgcat tacgttttca     6960

tggatacagg ttgtgaacat ccaatgacat atcggtttgt cagggaagtt gtgaagttct     7020

gggatatacc gctcaccgta ttgcaggttg atatcaaccc ggagcttgga cagccaaatg     7080

gttatacggt atgggaacca aaggatattc agacgcgaat gcctgttctg aagccattta     7140

tcgatatggt aaagaaatat ggcactccat acgtcggcgg cgcgttctgc actgacagat     7200

taaaactcgt tcccttcacc aaatactgtg atgaccattt cgggcgaggg aattacacca     7260

cgtggattgg catcagagct gatgaaccga agcggctaaa gccaaagcct ggaatcagat     7320

atcttgctga actgtcagac tttgagaagg aagatatcct cgcatggtgg aagcaacaac     7380

cattcgattt gcaaataccg gaacatctcg gtaactgcat attctgcatt aaaaaatcaa     7440

cgcaaaaaat cggacttgcc tgcaaagatg aggagggatt gcagcgtgtt tttaatgagg     7500

tcatcacggg atcccatgtg cgtgacggac atcgggaaac gccaaaggag attatgtacc     7560

gaggaagaat gtcgctggac ggtatcgcga aaatgtattc agaaaatgat tatcaagccc     7620

tgtatcagga catggtacga gctaaaagat tcgataccgg ctcttgttct gagtcatgcg     7680

aaatatttgg agggcagctt gatttcgact tcgggaggga agctgcatga tgcgatgtta     7740

tcggtgcggt gaatgcaaag aagataaccg cttccgacca aatcaacctt actggaatcg     7800

atggtgtctc cggtgtgaaa gaacaccaac aggggtgtta ccactaccgc aggaaaagga     7860

ggacgtgtgg cgagacagcg acgaagtatc accgacataa tctgcgaaaa ctgcaaatac     7920

cttccaacga aacgcaccag aaataaaccc aagccaatcc caaaagaatc tgacgtaaaa     7980

accttcaact acacggctca cctgtgggat atccggtggc taagacgtcg tgcgaggaaa     8040

acaaggtgat tgaccaaaat cgaagttacg aacaagaaag cgtcgagcga gctttaacgt     8100

gcgctaactg cggtcagaag ctgcatgtgc tggaagttca cgtgtgtgag cactgctgcg     8160

cagaactgat gagcgatccg aatagctcga tgcacgagga agaagatgat ggctaaacca     8220

gcgcgaagac gatgtaaaaa cgatgaatgc cgggaatggt ttcaccctgc attcgctaat     8280

cagtggtggt gctctccaga gtgtggaacc aagatagcac tcgaacgacg aagtaaagaa     8340

cgcgaaaaag cggaaaaagc agcagagaag aaacgacgac gagaggagca gaaacagaaa     8400

gataaactta agattcgaaa actcgcctta aagccccgca gttactggat taaacaagcc     8460

caacaagccg taaacgcctt catcagagaa agagaccgcg acttaccatg tatctcgtgc     8520

ggaacgctca cgtctgctca gtgggatgcc ggacattacc ggacaactgc tgcggcacct     8580

caactccgat ttaatgaacg caatattcac aagcaatgcg tggtgtgcaa ccagcacaaa     8640

agcggaaatc tcgttccgta tcgcgtcgaa ctgattagcc gcatcgggca ggaagcagta     8700

gacgaaatcg aatcaaacca taaccgccat cgctggacta tcgaagagtg caaggcgatc     8760

aaggcagagt accaacagaa actcaaagac ctgcgaaata gcagaagtga ggccgcatga     8820

cgttctcagt aaaaaccatt ccagacatgc tcgttgaagc atacggaaat cagacagaag     8880

tagcacgcag actgaaatgt agtcgcggta cggtcagaaa atacgttgat gataaagacg     8940

ggaaaatgca cgccatcgtc aacgacgttc tcatggttca tcgcggatgg agtgaaagag     9000

atgcgctatt acgaaaaaat tgatggcagc aaataccgaa atatttgggt agttggcgat     9060

ctgcacggat gctacacgaa cctgatgaac aaactggata cgattggatt cgacaacaaa     9120

aaagacctgc ttatctcggt gggcgatttg gttgatcgtg gtgcagagaa cgttgaatgc     9180

ctggaattaa tcacattccc ctggttcaga gctgtacgtg gaaaccatga gcaaatgatg     9240

attgatggct tatcagagcg tggaaacgtt aatcactggc tgcttaatgg cggtggctgg     9300

ttctttaatc tcgattacga caaagaaatt ctggctaaag ctcttgccca taaagcagat     9360

gaacttccgt taatcatcga actggtgagc aaagataaaa aatatgttat ctgccacgcc     9420

gattatccct ttgacgaata cgagtttgga aagccagttg atcatcagca ggtaatctgg     9480

aaccgcgaac gaatcagcaa ctcacaaaac gggatcgtga aagaaatcaa aggcgcggac     9540

acgttcatct ttggtcatac gccagcagtg aaaccactca agtttgccaa ccaaatgtat     9600

atcgataccg gcgcagtgtt ctgcggaaac ctaacattga ttcaggtaca gggagaaggc     9660

gcatgagact cgaaagcgta gctaaatttc attcgccaaa aagcccgatg atgagcgact     9720

caccacgggc cacggcttct gactctcttt ccggtactga tgtgatggct gctatgggga     9780

tggcgcaatc acaagccgga ttcggtatgg ctgcattctg cggtaagcac gaactcagcc     9840

agaacgacaa acaaaaggct atcaactatc tgatgcaatt tgcacacaag gtatcgggga     9900

aataccgtgg tgtggcaaag cttgaaggaa atactaaggc aaaggtactg caagtgctcg     9960

caacattcgc ttatgcggat tattgccgta gtgccgcgac gccgggggca agatgcagag    10020

attgccatgg tacaggccgt gcggttgata ttgccaaaac agagctgtgg gggagagttg    10080

tcgagaaaga gtgcggaaga tgcaaaggcg tcggctattc aaggatgcca gcaagcgcag    10140

catatcgcgc tgtgacgatg ctaatcccaa accttaccca acccacctgg tcacgcactg    10200

ttaagccgct gtatgacgct ctggtggtgc aatgccacaa agaagagtca atcgcagaca    10260

acattttgaa tgcggtcaca cgttagcagc atgattgcca cggatggcaa catattaacg    10320

gcatgatatt gacttattga ataaaattgg gtaaatttga ctcaacgatg ggttaattcg    10380

ctcgttgtgg tagtgagatg aaaagaggcg gcgcttacta ccgattccgc ctagttggtc    10440

acttcgacgt atcgtctgga actccaacca tcgcaggcag agaggtctgc aaaatgcaat    10500

cccgaaacag ttcgcaggta atagttagag cctgcataac ggtttcggga ttttttatat    10560

ctgcacaaca ggtaagagca ttgagtcgat aatcgtgaag agtcggcgag cctggttagc    10620

cagtgctctt tccgttgtgc tgaattaagc gaataccgga agcagaaccg gatcaccaaa    10680

tgcgtacagg cgtcatcgcc gcccagcaac agcacaaccc aaactgagcc gtagccactg    10740

tctgtcctga attcattagt aatagttacg ctgcggcctt ttacacatga ccttcgtgaa    10800

agcgggtggc aggaggtcgc gctaacaacc tcctgccgtt ttgcccgtgc atatcggtca    10860

cgaacaaatc tgattactaa acacagtagc ctggatttgt tctatcagta atcgacctta    10920

ttcctaatta aatagagcaa atccccttat tgggggtaag acatgaagat gccagaaaaa    10980

catgacctgt tggccgccat tctcgcggca aaggaacaag gcatcggggc aatccttgcg    11040

tttgcaatgg cgtaccttcg cggcagatat aatggcggtg cgtttacaaa aacagtaatc    11100

gacgcaacga tgtgcgccat tatcgcctgg ttcattcgtg accttctcga cttcgccgga    11160

ctaagtagca atctcgctta tataacgagc gtgtttatcg gctacatcgg tactgactcg    11220

attggttcgc ttatcaaacg cttcgctgct aaaaaagccg gagtagaaga tggtagaaat    11280

caataatcaa cgtaaggcgt tcctcgatat gctggcgtgg tcggagggaa ctgataacgg    11340

acgtcagaaa accagaaatc atggttatga cgtcattgta ggcggagagc tatttactga    11400

ttactccgat caccctcgca aacttgtcac gctaaaccca aaactcaaat caacaggcgc    11460

ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc    11520

cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc    11580

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc    11640

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc    11700

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag    11760

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc    11820

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt    11880

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct    11940

ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg    12000

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct    12060

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat    12120

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg    12180

ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa    12240

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt    12300

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc    12360

tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc    12420

atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttt                     12464


<210>  7
<211>  12656
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing the rAAV genome of p853


<220>
<221>  misc_feature
<222>  (9)..(803)
<223>  KanR Complement

<220>
<221>  misc_feature
<222>  (988)..(1162)
<223>  B1 B2 T1 Txn Terminator Complement

<220>
<221>  misc_feature
<222>  (1063)..(1079)
<223>  pTR

<220>
<221>  misc_feature
<222>  (1253)..(1382  )
<223>  AAV 5'  ITR

<220>
<221>  promoter
<222>  (1443)..(2714)

<220>
<221>  gene
<222>  (2715 )..(3062)
<223>  human hRdCVF short form optimized sequence

<220>
<221>  polyA_signal
<222>  (3057 )..(3283)

<220>
<221>  terminator
<222>  (3278 )..(3715)

<220>
<221>  promoter
<222>  (3710)..(5010)

<220>
<221>  gene
<222>  (4998 )..(5662)
<223>  hRDCVFL optimized sequence long form

<220>
<221>  polyA_signal
<222>  (5663 )..(5879)

<220>
<221>  misc_feature
<222>  (5927)..(6056)
<223>  AAV 3' ITR

<220>
<221>  terminator
<222>  (6150)..(6450)

<220>
<221>  misc_feature
<222>  (6241)..(6266)
<223>  pTF3 Complement

<220>
<221>  terminator
<222>  (6457)..(6570)

<220>
<221>  misc_feature
<222>  (6586)..(11652)
<223>  Lambda Stuffer

<220>
<221>  misc_feature
<222>  (11813)..(12616)
<223>  pUC Ori Complement

<400>  7
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggtcg acttaattaa ggctgcgcgc     1260

tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc     1320

ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc     1380

cttgtagtta atgattaacc cgccatgcta cttatctacg tagcaagcta gctagttatt     1440

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat     1500

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa     1560

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg     1620

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc     1680

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct     1740

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt aacatggtcg     1800

aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt     1860

tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc     1920

gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg     1980

cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc     2040

ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg     2100

ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt     2160

actcccacag gtgagcgggc gggatgagca cggcccggct tcgggtgcgg ggctccgtac     2220

ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg     2280

cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag     2340

cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga     2400

gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg     2460

ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg     2520

cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg     2580

ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg     2640

gcgtgtgacc ggcggctcta gacaattgta ctaaccttct tctctttcct ctcctgacag     2700

gttggtgtac actagcggcc gccaccatgg ccagcctctt ctccggacgc atcctgattc     2760

gcaacaattc cgaccaagac gaactggata ccgaggccga agtctcgcgg agattggaga     2820

acaggcttgt gctgctgttc tttggcgcgg gagcgtgtcc tcagtgccag gctttcgtgc     2880

caatcctgaa ggatttcttc gtgcggctga ctgacgaatt ctacgtcctc cgggccgccc     2940

agctggcact ggtgtacgtg tcccaagact caaccgagga acagcaggat ctgttcctca     3000

aggacatgcc caaaaagtgg ctgttcctgc cgtttgagga cgacttgcgg cgctagtgat     3060

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt     3120

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat     3180

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg     3240

gggaggattg ggaagacaat agcaggcatg ctggggagga tccaatttat tctaaatgca     3300

taataaatac tgataacatc ttatagtttg tattatattt tgtattatcg ttgacatgta     3360

taattttgat atcaaaaact gattttccct ttattatttt cgagatttat tttcttaatt     3420

ctctttaaca aactagaaat attgtatata caaaaaatca taaataatag atgaatagtt     3480

taattatagg tgttcatcaa tcgaaaaagc aacgtatctt atttaaagtg cgttgctttt     3540

ttctcattta taaggttaaa taattctcat atatcaagca aagtgacagg cgcccttaaa     3600

tattctgaca aatgctcttt ccctaaactc cccccataaa aaaacccgcc gaagcgggtt     3660

tttacgttat ttgcggatta acgattactc gttatcagaa ccgcccagga agctttagtt     3720

attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta     3780

cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt     3840

caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg     3900

tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta     3960

cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga     4020

ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaacatgg     4080

tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa     4140

ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg     4200

ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg     4260

cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc     4320

ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg gagtcgctgc gacgctgcct     4380

tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc     4440

gttactccca caggtgagcg ggcgggatga gcacggcccg gcttcgggtg cggggctccg     4500

tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc     4560

gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg     4620

gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg     4680

cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg     4740

ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat     4800

gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg     4860

gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt     4920

ctggcgtgtg accggcggct ctagacaatt gtactaacct tcttctcttt cctctcctga     4980

caggttggtg tacactaggc catacaggcc gccaccatgg cctcactgtt ctccgggcgc     5040

atcctcatcc gaaacaacag cgatcaggac gaattggaca ccgaggctga agtctcccgc     5100

cggctggaaa acaggctcgt gctcctgttc ttcggtgccg gagcgtgccc gcagtgccaa     5160

gccttcgtcc caattcttaa ggacttcttt gtgcgcctca ctgatgagtt ttacgtgctc     5220

cgggcagcgc agctggcctt ggtgtatgtg tcgcaagatt ccactgagga acaacaggac     5280

ctgttcctga aagacatgcc taagaagtgg cttttcctgc ccttcgagga cgacctgaga     5340

agggacctgg gacgccagtt cagcgtggaa cggctgccgg ccgtcgtggt gctgaagccc     5400

gacggggacg tgcttacccg ggatggcgct gacgaaatcc agaggctggg caccgcctgt     5460

ttcgcaaatt ggcaggaggc cgccgaagtg ctcgaccgga acttccagct gcccgaggat     5520

ctggaggacc aggaacctcg gtccctgacc gagtgcctca gacgccacaa gtaccgcgtg     5580

gaaaaggccg cgagaggagg acgggacccg ggtggcgggg gaggcgaaga gggcggagcc     5640

ggtggcctgt tctgatagat ctgcctcgac tgtgccttct agttgccagc catctgttgt     5700

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     5760

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg     5820

ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggactc     5880

gagttctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg     5940

atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag     6000

gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt     6060

aattaaccta aggaaaatga agtgaagttc ctatactttc tagagaatag gaacttctat     6120

agtgagtcga ataagggcga cacaaaattt attctaaatg cataataaat actgataaca     6180

tcttatagtt tgtattatat tttgtattat cgttgacatg tataattttg atatcaaaaa     6240

ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa caaactagaa     6300

atattgtata tacaaaaaat cataaataat agatgaatag tttaattata ggtgttcatc     6360

aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt tataaggtta     6420

aataattctc atatatcaag caaagtgaca ggcgccctta aatattctga caaatgctct     6480

ttccctaaac tccccccata aaaaaacccg ccgaagcggg tttttacgtt atttgcggat     6540

taacgattac tcgttatcag aaccgcccag ggggcccgag cttaaccttt ttatttgggg     6600

gagagggaag tcatgaaaaa actaaccttt gaaattcgat ctccagcaca tcagcaaaac     6660

gctattcacg cagtacagca aatccttcca gacccaacca aaccaatcgt agtaaccatt     6720

caggaacgca accgcagctt agaccaaaac aggaagctat gggcctgctt aggtgacgtc     6780

tctcgtcagg ttgaatggca tggtcgctgg ctggatgcag aaagctggaa gtgtgtgttt     6840

accgcagcat taaagcagca ggatgttgtt cctaaccttg ccgggaatgg ctttgtggta     6900

ataggccagt caaccagcag gatgcgtgta ggcgaatttg cggagctatt agagcttata     6960

caggcattcg gtacagagcg tggcgttaag tggtcagacg aagcgagact ggctctggag     7020

tggaaagcga gatggggaga cagggctgca tgataaatgt cgttagtttc tccggtggca     7080

ggacgtcagc atatttgctc tggctaatgg agcaaaagcg acgggcaggt aaagacgtgc     7140

attacgtttt catggataca ggttgtgaac atccaatgac atatcggttt gtcagggaag     7200

ttgtgaagtt ctgggatata ccgctcaccg tattgcaggt tgatatcaac ccggagcttg     7260

gacagccaaa tggttatacg gtatgggaac caaaggatat tcagacgcga atgcctgttc     7320

tgaagccatt tatcgatatg gtaaagaaat atggcactcc atacgtcggc ggcgcgttct     7380

gcactgacag attaaaactc gttcccttca ccaaatactg tgatgaccat ttcgggcgag     7440

ggaattacac cacgtggatt ggcatcagag ctgatgaacc gaagcggcta aagccaaagc     7500

ctggaatcag atatcttgct gaactgtcag actttgagaa ggaagatatc ctcgcatggt     7560

ggaagcaaca accattcgat ttgcaaatac cggaacatct cggtaactgc atattctgca     7620

ttaaaaaatc aacgcaaaaa atcggacttg cctgcaaaga tgaggaggga ttgcagcgtg     7680

tttttaatga ggtcatcacg ggatcccatg tgcgtgacgg acatcgggaa acgccaaagg     7740

agattatgta ccgaggaaga atgtcgctgg acggtatcgc gaaaatgtat tcagaaaatg     7800

attatcaagc cctgtatcag gacatggtac gagctaaaag attcgatacc ggctcttgtt     7860

ctgagtcatg cgaaatattt ggagggcagc ttgatttcga cttcgggagg gaagctgcat     7920

gatgcgatgt tatcggtgcg gtgaatgcaa agaagataac cgcttccgac caaatcaacc     7980

ttactggaat cgatggtgtc tccggtgtga aagaacacca acaggggtgt taccactacc     8040

gcaggaaaag gaggacgtgt ggcgagacag cgacgaagta tcaccgacat aatctgcgaa     8100

aactgcaaat accttccaac gaaacgcacc agaaataaac ccaagccaat cccaaaagaa     8160

tctgacgtaa aaaccttcaa ctacacggct cacctgtggg atatccggtg gctaagacgt     8220

cgtgcgagga aaacaaggtg attgaccaaa atcgaagtta cgaacaagaa agcgtcgagc     8280

gagctttaac gtgcgctaac tgcggtcaga agctgcatgt gctggaagtt cacgtgtgtg     8340

agcactgctg cgcagaactg atgagcgatc cgaatagctc gatgcacgag gaagaagatg     8400

atggctaaac cagcgcgaag acgatgtaaa aacgatgaat gccgggaatg gtttcaccct     8460

gcattcgcta atcagtggtg gtgctctcca gagtgtggaa ccaagatagc actcgaacga     8520

cgaagtaaag aacgcgaaaa agcggaaaaa gcagcagaga agaaacgacg acgagaggag     8580

cagaaacaga aagataaact taagattcga aaactcgcct taaagccccg cagttactgg     8640

attaaacaag cccaacaagc cgtaaacgcc ttcatcagag aaagagaccg cgacttacca     8700

tgtatctcgt gcggaacgct cacgtctgct cagtgggatg ccggacatta ccggacaact     8760

gctgcggcac ctcaactccg atttaatgaa cgcaatattc acaagcaatg cgtggtgtgc     8820

aaccagcaca aaagcggaaa tctcgttccg tatcgcgtcg aactgattag ccgcatcggg     8880

caggaagcag tagacgaaat cgaatcaaac cataaccgcc atcgctggac tatcgaagag     8940

tgcaaggcga tcaaggcaga gtaccaacag aaactcaaag acctgcgaaa tagcagaagt     9000

gaggccgcat gacgttctca gtaaaaacca ttccagacat gctcgttgaa gcatacggaa     9060

atcagacaga agtagcacgc agactgaaat gtagtcgcgg tacggtcaga aaatacgttg     9120

atgataaaga cgggaaaatg cacgccatcg tcaacgacgt tctcatggtt catcgcggat     9180

ggagtgaaag agatgcgcta ttacgaaaaa attgatggca gcaaataccg aaatatttgg     9240

gtagttggcg atctgcacgg atgctacacg aacctgatga acaaactgga tacgattgga     9300

ttcgacaaca aaaaagacct gcttatctcg gtgggcgatt tggttgatcg tggtgcagag     9360

aacgttgaat gcctggaatt aatcacattc ccctggttca gagctgtacg tggaaaccat     9420

gagcaaatga tgattgatgg cttatcagag cgtggaaacg ttaatcactg gctgcttaat     9480

ggcggtggct ggttctttaa tctcgattac gacaaagaaa ttctggctaa agctcttgcc     9540

cataaagcag atgaacttcc gttaatcatc gaactggtga gcaaagataa aaaatatgtt     9600

atctgccacg ccgattatcc ctttgacgaa tacgagtttg gaaagccagt tgatcatcag     9660

caggtaatct ggaaccgcga acgaatcagc aactcacaaa acgggatcgt gaaagaaatc     9720

aaaggcgcgg acacgttcat ctttggtcat acgccagcag tgaaaccact caagtttgcc     9780

aaccaaatgt atatcgatac cggcgcagtg ttctgcggaa acctaacatt gattcaggta     9840

cagggagaag gcgcatgaga ctcgaaagcg tagctaaatt tcattcgcca aaaagcccga     9900

tgatgagcga ctcaccacgg gccacggctt ctgactctct ttccggtact gatgtgatgg     9960

ctgctatggg gatggcgcaa tcacaagccg gattcggtat ggctgcattc tgcggtaagc    10020

acgaactcag ccagaacgac aaacaaaagg ctatcaacta tctgatgcaa tttgcacaca    10080

aggtatcggg gaaataccgt ggtgtggcaa agcttgaagg aaatactaag gcaaaggtac    10140

tgcaagtgct cgcaacattc gcttatgcgg attattgccg tagtgccgcg acgccggggg    10200

caagatgcag agattgccat ggtacaggcc gtgcggttga tattgccaaa acagagctgt    10260

gggggagagt tgtcgagaaa gagtgcggaa gatgcaaagg cgtcggctat tcaaggatgc    10320

cagcaagcgc agcatatcgc gctgtgacga tgctaatccc aaaccttacc caacccacct    10380

ggtcacgcac tgttaagccg ctgtatgacg ctctggtggt gcaatgccac aaagaagagt    10440

caatcgcaga caacattttg aatgcggtca cacgttagca gcatgattgc cacggatggc    10500

aacatattaa cggcatgata ttgacttatt gaataaaatt gggtaaattt gactcaacga    10560

tgggttaatt cgctcgttgt ggtagtgaga tgaaaagagg cggcgcttac taccgattcc    10620

gcctagttgg tcacttcgac gtatcgtctg gaactccaac catcgcaggc agagaggtct    10680

gcaaaatgca atcccgaaac agttcgcagg taatagttag agcctgcata acggtttcgg    10740

gattttttat atctgcacaa caggtaagag cattgagtcg ataatcgtga agagtcggcg    10800

agcctggtta gccagtgctc tttccgttgt gctgaattaa gcgaataccg gaagcagaac    10860

cggatcacca aatgcgtaca ggcgtcatcg ccgcccagca acagcacaac ccaaactgag    10920

ccgtagccac tgtctgtcct gaattcatta gtaatagtta cgctgcggcc ttttacacat    10980

gaccttcgtg aaagcgggtg gcaggaggtc gcgctaacaa cctcctgccg ttttgcccgt    11040

gcatatcggt cacgaacaaa tctgattact aaacacagta gcctggattt gttctatcag    11100

taatcgacct tattcctaat taaatagagc aaatcccctt attgggggta agacatgaag    11160

atgccagaaa aacatgacct gttggccgcc attctcgcgg caaaggaaca aggcatcggg    11220

gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat ataatggcgg tgcgtttaca    11280

aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct ggttcattcg tgaccttctc    11340

gacttcgccg gactaagtag caatctcgct tatataacga gcgtgtttat cggctacatc    11400

ggtactgact cgattggttc gcttatcaaa cgcttcgctg ctaaaaaagc cggagtagaa    11460

gatggtagaa atcaataatc aacgtaaggc gttcctcgat atgctggcgt ggtcggaggg    11520

aactgataac ggacgtcaga aaaccagaaa tcatggttat gacgtcattg taggcggaga    11580

gctatttact gattactccg atcaccctcg caaacttgtc acgctaaacc caaaactcaa    11640

atcaacaggc gcttaagact ggccgtcgtt ttacaacaca gaaagagttt gtagaaacgc    11700

aaaaaggcca tccgtcaggg gccttctgct tagtttgatg cctggcagtt ccctactctc    11760

gccttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt    11820

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa    11880

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc    11940

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag    12000

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt    12060

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg    12120

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg    12180

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg    12240

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac    12300

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg    12360

ggctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt    12420

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg    12480

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc    12540

tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgcgcgtaa ctcacgttaa    12600

gggattttgg tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgcttt        12656


<210>  8
<211>  4727
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rAAV genome  expressing two copies of human RdCVF codon optimized
       short form sequences


<220>
<221>  misc_feature
<222>  (9)..(100)
<223>  KanR complement

<220>
<221>  misc_feature
<222>  (117)..(246)
<223>  AAV 5'  ITR

<220>
<221>  promoter
<222>  (307)..(1578)

<220>
<221>  gene
<222>  (1579)..(1926)
<223>  human optimized RdCVF short form

<220>
<221>  polyA_signal
<222>  (1931)..(2152)

<220>
<221>  terminator
<222>  (2237)..(2537)

<220>
<221>  misc_feature
<222>  (2328)..(2353)
<223>  pTF3 complement

<220>
<221>  terminator
<222>  (2544)..(2657)

<220>
<221>  promoter
<222>  (2688)..(3959)

<220>
<221>  gene
<222>  (3960)..(4307)
<223>  human optimized RdCVF short form second copy

<220>
<221>  polyA_signal
<222>  (4312)..(4533)

<220>
<221>  misc_feature
<222>  (4581)..(4710)
<223>  AAV 3' ITR

<400>  8
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa gtcgacttaa ttaaggctgc      120

gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc      180

gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg      240

gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcaa gctagctagt      300

tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt      360

acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg      420

tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg      480

gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt      540

acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg      600

accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaacatg      660

gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca      720

attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg      780

gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt      840

gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg      900

cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg ggagtcgctg cgacgctgcc      960

ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg     1020

cgttactccc acaggtgagc gggcgggatg agcacggccc ggcttcgggt gcggggctcc     1080

gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc     1140

cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc     1200

ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt     1260

gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc     1320

gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa     1380

tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc     1440

ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct     1500

tctggcgtgt gaccggcggc tctagacaat tgtactaacc ttcttctctt tcctctcctg     1560

acaggttggt gtacactagc ggccgccacc atggccagcc tcttctccgg acgcatcctg     1620

attcgcaaca attccgacca agacgaactg gataccgagg ccgaagtctc gcggagattg     1680

gagaacaggc ttgtgctgct gttctttggc gcgggagcgt gtcctcagtg ccaggctttc     1740

gtgccaatcc tgaaggattt cttcgtgcgg ctgactgacg aattctacgt cctccgggcc     1800

gcccagctgg cactggtgta cgtgtcccaa gactcaaccg aggaacagca ggatctgttc     1860

ctcaaggaca tgcccaaaaa gtggctgttc ctgccgtttg aggacgactt gcggcgctag     1920

tgatcaaaga gatctgcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc     1980

tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat     2040

gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg     2100

caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga ctcgataagg     2160

aaaatgaagt gaagttccta tactttctag agaataggaa cttctatagt gagtcgaata     2220

agggcgacac aaaatttatt ctaaatgcat aataaatact gataacatct tatagtttgt     2280

attatatttt gtattatcgt tgacatgtat aattttgata tcaaaaactg attttccctt     2340

tattattttc gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac     2400

aaaaaatcat aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca     2460

acgtatctta tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata     2520

tatcaagcaa agtgacaggc gcccttaaat attctgacaa atgctctttc cctaaactcc     2580

ccccataaaa aaacccgccg aagcgggttt ttacgttatt tgcggattaa cgattactcg     2640

ttatcagaac cgcccagggg gcccgagctt aactagctag ttattaatag taatcaatta     2700

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg     2760

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc     2820

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa     2880

ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca     2940

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta     3000

cttggcagta catctacgta ttagtcatcg ctattaacat ggtcgaggtg agccccacgt     3060

tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt     3120

tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccaggcgggg     3180

cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga     3240

gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa     3300

agcgaagcgc gcggcgggcg gggagtcgct gcgacgctgc cttcgccccg tgccccgctc     3360

cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag     3420

cgggcgggat gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg     3480

gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc     3540

tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg     3600

aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact     3660

tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag     3720

cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg     3780

tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga     3840

cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg     3900

ctctagacaa ttgtactaac cttcttctct ttcctctcct gacaggttgg tgtacactag     3960

cggccgccac catggccagc ctcttctccg gacgcatcct gattcgcaac aattccgacc     4020

aagacgaact ggataccgag gccgaagtct cgcggagatt ggagaacagg cttgtgctgc     4080

tgttctttgg cgcgggagcg tgtcctcagt gccaggcttt cgtgccaatc ctgaaggatt     4140

tcttcgtgcg gctgactgac gaattctacg tcctccgggc cgcccagctg gcactggtgt     4200

acgtgtccca agactcaacc gaggaacagc aggatctgtt cctcaaggac atgcccaaaa     4260

agtggctgtt cctgccgttt gaggacgact tgcggcgcta gtgatcaaag agatctgcct     4320

cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga     4380

ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt     4440

gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg     4500

attgggaaga caatagcagg catgctgggg actcgagttc tacgtagata agtagcatgg     4560

cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg     4620

cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg     4680

ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaagg                   4727


<210>  9
<211>  12446
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid expressing rAAV genome with two copies of optimized human
       RdCVF short form


<220>
<221>  misc_feature
<222>  (9)..(803)
<223>  KanR complement

<220>
<221>  misc_feature
<222>  (988)..(1162)
<223>  B1 B2 T1 Txn Terminator Complement

<220>
<221>  misc_feature
<222>  (1063)..(1079)
<223>  pTR

<220>
<221>  misc_feature
<222>  (1253)..(1382)
<223>  5' AAV ITR

<220>
<221>  promoter
<222>  (1443)..(2714)

<220>
<221>  gene
<222>  (2715)..(3062)
<223>  human optimized RdCVF short form

<220>
<221>  polyA_signal
<222>  (3067)..(3288)

<220>
<221>  terminator
<222>  (3373)..(3673)

<220>
<221>  misc_feature
<222>  (3464)..(3489)
<223>  pTF3 complement

<220>
<221>  terminator
<222>  (3680)..(3793)

<220>
<221>  promoter
<222>  (3824)..(5095)

<220>
<221>  gene
<222>  (5096)..(5443)
<223>  human optimized RdCVF short form second copy

<220>
<221>  polyA_signal
<222>  (5448)..(5669)

<220>
<221>  misc_feature
<222>  (5717)..(5846)
<223>  AAV 3' ITR

<220>
<221>  terminator
<222>  (5940)..(6240)

<220>
<221>  misc_feature
<222>  (6031)..(6056)
<223>  pTF3 Complement

<220>
<221>  terminator
<222>  (6247)..(6360)

<220>
<221>  misc_feature
<222>  (6376)..(11442)
<223>  Lambda Stuffer

<220>
<221>  misc_feature
<222>  (11603)..(12406)
<223>  pUC Ori Complement

<400>  9
tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata       60

ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat      120

aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct      180

attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact      240

gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag      300

ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc      360

gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgag      420

tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat      480

tcttctaata cctggaacgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca      540

tcaggagtac ggataaaatg cttgatggtc ggaagtggca taaattccgt cagccagttt      600

agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac      660

aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca      720

ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc      780

ctcgacgttt cccgttgaat atggctcata ttcttccttt ttcaatatta ttgaagcatt      840

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa      900

ataggggtca gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata      960

cctgaatatg gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc     1020

tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc     1080

ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact     1140

gggcctttcg cccgggctaa ttagggggtg tcgcccttat tcgactctat agtgaagttc     1200

ctattctcta gaaagtatag gaacttctga agtggggtcg acttaattaa ggctgcgcgc     1260

tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc     1320

ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc     1380

cttgtagtta atgattaacc cgccatgcta cttatctacg tagcaagcta gctagttatt     1440

aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat     1500

aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa     1560

taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg     1620

agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc     1680

cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct     1740

tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt aacatggtcg     1800

aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt     1860

tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc     1920

gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg     1980

cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc     2040

ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac gctgccttcg     2100

ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt     2160

actcccacag gtgagcgggc gggatgagca cggcccggct tcgggtgcgg ggctccgtac     2220

ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg     2280

cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag     2340

cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga     2400

gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg     2460

ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg     2520

cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg     2580

ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg     2640

gcgtgtgacc ggcggctcta gacaattgta ctaaccttct tctctttcct ctcctgacag     2700

gttggtgtac actagcggcc gccaccatgg ccagcctctt ctccggacgc atcctgattc     2760

gcaacaattc cgaccaagac gaactggata ccgaggccga agtctcgcgg agattggaga     2820

acaggcttgt gctgctgttc tttggcgcgg gagcgtgtcc tcagtgccag gctttcgtgc     2880

caatcctgaa ggatttcttc gtgcggctga ctgacgaatt ctacgtcctc cgggccgccc     2940

agctggcact ggtgtacgtg tcccaagact caaccgagga acagcaggat ctgttcctca     3000

aggacatgcc caaaaagtgg ctgttcctgc cgtttgagga cgacttgcgg cgctagtgat     3060

caaagagatc tgcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc     3120

ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg     3180

aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg     3240

acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggactcg ataaggaaaa     3300

tgaagtgaag ttcctatact ttctagagaa taggaacttc tatagtgagt cgaataaggg     3360

cgacacaaaa tttattctaa atgcataata aatactgata acatcttata gtttgtatta     3420

tattttgtat tatcgttgac atgtataatt ttgatatcaa aaactgattt tccctttatt     3480

attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa     3540

aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt     3600

atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc     3660

aagcaaagtg acaggcgccc ttaaatattc tgacaaatgc tctttcccta aactcccccc     3720

ataaaaaaac ccgccgaagc gggtttttac gttatttgcg gattaacgat tactcgttat     3780

cagaaccgcc cagggggccc gagcttaact agctagttat taatagtaat caattacggg     3840

gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc     3900

gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat     3960

agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc     4020

ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga     4080

cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg     4140

gcagtacatc tacgtattag tcatcgctat taacatggtc gaggtgagcc ccacgttctg     4200

cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta     4260

attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg     4320

gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg     4380

cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg     4440

aagcgcgcgg cgggcgggga gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc     4500

gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg     4560

cgggatgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc     4620

gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg     4680

gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc     4740

gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct     4800

ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg     4860

cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg     4920

tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc     4980

tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct     5040

agacaattgt actaaccttc ttctctttcc tctcctgaca ggttggtgta cactagcggc     5100

cgccaccatg gccagcctct tctccggacg catcctgatt cgcaacaatt ccgaccaaga     5160

cgaactggat accgaggccg aagtctcgcg gagattggag aacaggcttg tgctgctgtt     5220

ctttggcgcg ggagcgtgtc ctcagtgcca ggctttcgtg ccaatcctga aggatttctt     5280

cgtgcggctg actgacgaat tctacgtcct ccgggccgcc cagctggcac tggtgtacgt     5340

gtcccaagac tcaaccgagg aacagcagga tctgttcctc aaggacatgc ccaaaaagtg     5400

gctgttcctg ccgtttgagg acgacttgcg gcgctagtga tcaaagagat ctgcctcgac     5460

tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct     5520

ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct     5580

gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg     5640

ggaagacaat agcaggcatg ctggggactc gagttctacg tagataagta gcatggcggg     5700

ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct     5760

cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg     5820

gcctcagtga gcgagcgagc gcgcagcctt aattaaccta aggaaaatga agtgaagttc     5880

ctatactttc tagagaatag gaacttctat agtgagtcga ataagggcga cacaaaattt     5940

attctaaatg cataataaat actgataaca tcttatagtt tgtattatat tttgtattat     6000

cgttgacatg tataattttg atatcaaaaa ctgattttcc ctttattatt ttcgagattt     6060

attttcttaa ttctctttaa caaactagaa atattgtata tacaaaaaat cataaataat     6120

agatgaatag tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag     6180

tgcgttgctt ttttctcatt tataaggtta aataattctc atatatcaag caaagtgaca     6240

ggcgccctta aatattctga caaatgctct ttccctaaac tccccccata aaaaaacccg     6300

ccgaagcggg tttttacgtt atttgcggat taacgattac tcgttatcag aaccgcccag     6360

ggggcccgag cttaaccttt ttatttgggg gagagggaag tcatgaaaaa actaaccttt     6420

gaaattcgat ctccagcaca tcagcaaaac gctattcacg cagtacagca aatccttcca     6480

gacccaacca aaccaatcgt agtaaccatt caggaacgca accgcagctt agaccaaaac     6540

aggaagctat gggcctgctt aggtgacgtc tctcgtcagg ttgaatggca tggtcgctgg     6600

ctggatgcag aaagctggaa gtgtgtgttt accgcagcat taaagcagca ggatgttgtt     6660

cctaaccttg ccgggaatgg ctttgtggta ataggccagt caaccagcag gatgcgtgta     6720

ggcgaatttg cggagctatt agagcttata caggcattcg gtacagagcg tggcgttaag     6780

tggtcagacg aagcgagact ggctctggag tggaaagcga gatggggaga cagggctgca     6840

tgataaatgt cgttagtttc tccggtggca ggacgtcagc atatttgctc tggctaatgg     6900

agcaaaagcg acgggcaggt aaagacgtgc attacgtttt catggataca ggttgtgaac     6960

atccaatgac atatcggttt gtcagggaag ttgtgaagtt ctgggatata ccgctcaccg     7020

tattgcaggt tgatatcaac ccggagcttg gacagccaaa tggttatacg gtatgggaac     7080

caaaggatat tcagacgcga atgcctgttc tgaagccatt tatcgatatg gtaaagaaat     7140

atggcactcc atacgtcggc ggcgcgttct gcactgacag attaaaactc gttcccttca     7200

ccaaatactg tgatgaccat ttcgggcgag ggaattacac cacgtggatt ggcatcagag     7260

ctgatgaacc gaagcggcta aagccaaagc ctggaatcag atatcttgct gaactgtcag     7320

actttgagaa ggaagatatc ctcgcatggt ggaagcaaca accattcgat ttgcaaatac     7380

cggaacatct cggtaactgc atattctgca ttaaaaaatc aacgcaaaaa atcggacttg     7440

cctgcaaaga tgaggaggga ttgcagcgtg tttttaatga ggtcatcacg ggatcccatg     7500

tgcgtgacgg acatcgggaa acgccaaagg agattatgta ccgaggaaga atgtcgctgg     7560

acggtatcgc gaaaatgtat tcagaaaatg attatcaagc cctgtatcag gacatggtac     7620

gagctaaaag attcgatacc ggctcttgtt ctgagtcatg cgaaatattt ggagggcagc     7680

ttgatttcga cttcgggagg gaagctgcat gatgcgatgt tatcggtgcg gtgaatgcaa     7740

agaagataac cgcttccgac caaatcaacc ttactggaat cgatggtgtc tccggtgtga     7800

aagaacacca acaggggtgt taccactacc gcaggaaaag gaggacgtgt ggcgagacag     7860

cgacgaagta tcaccgacat aatctgcgaa aactgcaaat accttccaac gaaacgcacc     7920

agaaataaac ccaagccaat cccaaaagaa tctgacgtaa aaaccttcaa ctacacggct     7980

cacctgtggg atatccggtg gctaagacgt cgtgcgagga aaacaaggtg attgaccaaa     8040

atcgaagtta cgaacaagaa agcgtcgagc gagctttaac gtgcgctaac tgcggtcaga     8100

agctgcatgt gctggaagtt cacgtgtgtg agcactgctg cgcagaactg atgagcgatc     8160

cgaatagctc gatgcacgag gaagaagatg atggctaaac cagcgcgaag acgatgtaaa     8220

aacgatgaat gccgggaatg gtttcaccct gcattcgcta atcagtggtg gtgctctcca     8280

gagtgtggaa ccaagatagc actcgaacga cgaagtaaag aacgcgaaaa agcggaaaaa     8340

gcagcagaga agaaacgacg acgagaggag cagaaacaga aagataaact taagattcga     8400

aaactcgcct taaagccccg cagttactgg attaaacaag cccaacaagc cgtaaacgcc     8460

ttcatcagag aaagagaccg cgacttacca tgtatctcgt gcggaacgct cacgtctgct     8520

cagtgggatg ccggacatta ccggacaact gctgcggcac ctcaactccg atttaatgaa     8580

cgcaatattc acaagcaatg cgtggtgtgc aaccagcaca aaagcggaaa tctcgttccg     8640

tatcgcgtcg aactgattag ccgcatcggg caggaagcag tagacgaaat cgaatcaaac     8700

cataaccgcc atcgctggac tatcgaagag tgcaaggcga tcaaggcaga gtaccaacag     8760

aaactcaaag acctgcgaaa tagcagaagt gaggccgcat gacgttctca gtaaaaacca     8820

ttccagacat gctcgttgaa gcatacggaa atcagacaga agtagcacgc agactgaaat     8880

gtagtcgcgg tacggtcaga aaatacgttg atgataaaga cgggaaaatg cacgccatcg     8940

tcaacgacgt tctcatggtt catcgcggat ggagtgaaag agatgcgcta ttacgaaaaa     9000

attgatggca gcaaataccg aaatatttgg gtagttggcg atctgcacgg atgctacacg     9060

aacctgatga acaaactgga tacgattgga ttcgacaaca aaaaagacct gcttatctcg     9120

gtgggcgatt tggttgatcg tggtgcagag aacgttgaat gcctggaatt aatcacattc     9180

ccctggttca gagctgtacg tggaaaccat gagcaaatga tgattgatgg cttatcagag     9240

cgtggaaacg ttaatcactg gctgcttaat ggcggtggct ggttctttaa tctcgattac     9300

gacaaagaaa ttctggctaa agctcttgcc cataaagcag atgaacttcc gttaatcatc     9360

gaactggtga gcaaagataa aaaatatgtt atctgccacg ccgattatcc ctttgacgaa     9420

tacgagtttg gaaagccagt tgatcatcag caggtaatct ggaaccgcga acgaatcagc     9480

aactcacaaa acgggatcgt gaaagaaatc aaaggcgcgg acacgttcat ctttggtcat     9540

acgccagcag tgaaaccact caagtttgcc aaccaaatgt atatcgatac cggcgcagtg     9600

ttctgcggaa acctaacatt gattcaggta cagggagaag gcgcatgaga ctcgaaagcg     9660

tagctaaatt tcattcgcca aaaagcccga tgatgagcga ctcaccacgg gccacggctt     9720

ctgactctct ttccggtact gatgtgatgg ctgctatggg gatggcgcaa tcacaagccg     9780

gattcggtat ggctgcattc tgcggtaagc acgaactcag ccagaacgac aaacaaaagg     9840

ctatcaacta tctgatgcaa tttgcacaca aggtatcggg gaaataccgt ggtgtggcaa     9900

agcttgaagg aaatactaag gcaaaggtac tgcaagtgct cgcaacattc gcttatgcgg     9960

attattgccg tagtgccgcg acgccggggg caagatgcag agattgccat ggtacaggcc    10020

gtgcggttga tattgccaaa acagagctgt gggggagagt tgtcgagaaa gagtgcggaa    10080

gatgcaaagg cgtcggctat tcaaggatgc cagcaagcgc agcatatcgc gctgtgacga    10140

tgctaatccc aaaccttacc caacccacct ggtcacgcac tgttaagccg ctgtatgacg    10200

ctctggtggt gcaatgccac aaagaagagt caatcgcaga caacattttg aatgcggtca    10260

cacgttagca gcatgattgc cacggatggc aacatattaa cggcatgata ttgacttatt    10320

gaataaaatt gggtaaattt gactcaacga tgggttaatt cgctcgttgt ggtagtgaga    10380

tgaaaagagg cggcgcttac taccgattcc gcctagttgg tcacttcgac gtatcgtctg    10440

gaactccaac catcgcaggc agagaggtct gcaaaatgca atcccgaaac agttcgcagg    10500

taatagttag agcctgcata acggtttcgg gattttttat atctgcacaa caggtaagag    10560

cattgagtcg ataatcgtga agagtcggcg agcctggtta gccagtgctc tttccgttgt    10620

gctgaattaa gcgaataccg gaagcagaac cggatcacca aatgcgtaca ggcgtcatcg    10680

ccgcccagca acagcacaac ccaaactgag ccgtagccac tgtctgtcct gaattcatta    10740

gtaatagtta cgctgcggcc ttttacacat gaccttcgtg aaagcgggtg gcaggaggtc    10800

gcgctaacaa cctcctgccg ttttgcccgt gcatatcggt cacgaacaaa tctgattact    10860

aaacacagta gcctggattt gttctatcag taatcgacct tattcctaat taaatagagc    10920

aaatcccctt attgggggta agacatgaag atgccagaaa aacatgacct gttggccgcc    10980

attctcgcgg caaaggaaca aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt    11040

cgcggcagat ataatggcgg tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc    11100

attatcgcct ggttcattcg tgaccttctc gacttcgccg gactaagtag caatctcgct    11160

tatataacga gcgtgtttat cggctacatc ggtactgact cgattggttc gcttatcaaa    11220

cgcttcgctg ctaaaaaagc cggagtagaa gatggtagaa atcaataatc aacgtaaggc    11280

gttcctcgat atgctggcgt ggtcggaggg aactgataac ggacgtcaga aaaccagaaa    11340

tcatggttat gacgtcattg taggcggaga gctatttact gattactccg atcaccctcg    11400

caaacttgtc acgctaaacc caaaactcaa atcaacaggc gcttaagact ggccgtcgtt    11460

ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct    11520

tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca ctgactcgct    11580

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt    11640

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc    11700

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga    11760

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata    11820

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac    11880

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg    11940

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc    12000

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag    12060

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt    12120

aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt    12180

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg    12240

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac    12300

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca    12360

gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc    12420

gtcaagtcag cgtaatgctc tgcttt                                         12446


