                         SEQUENCE LISTING

<110>  The University of California
 
<120>  Use of Medea elements for biocontrol of D. suzukii populations

<130>  1279-611-XPC

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  214
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA for targeting Drosophila suzukii myd88 gene

<400>  1
tttaaagtcc acaactcatc aaggaaaatg aaagtcaaag ttggcagctt acttaaactt       60

aatcacagcc tttaatgtaa aattaaaaaa aaatagtact ataagttaat ataccatatc      120

tatattacta ttttttttta attttgtacc taaagtgcct aacatcatta tttaattttt      180

tttttttttg gcacacgaat aaccatgccg tttt                                  214


<210>  2
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA for targeting Drosophila suzukii myd88 gene

<400>  2
ctttaaagtc cacaactcat caaggaaaat gaaagtcaaa gttggcagct tacttaaact       60

taatcacagc ctttaatgtt cccgcgcttc atcgttttct ttaagttaat ataccatatc      120

taaataaaac gatgaagcgc gggagtacct aaagtgccta acatcattat ttaatttttt      180

ttttttttgg cacacgaata accatgccgt ttt                                   213


<210>  3
<211>  213
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miRNA sequence for targeting Drosophila suzukii myd88 gene

<400>  3
atttaaagtc cacaactcat caaggaaaat gaaagtcaaa gttggcagct tacttaaact       60

taatcacagc ctttaatgta cgtcccgttg ataaatacct ataagttaat ataccatatc      120

tatatgtatt tatcaacggg acgtgtacct aaagtgccta acatcattat ttaatttttt      180

ttttttttgg cacacgaata accatgccgt ttt                                   213


<210>  4
<211>  2632
<212>  DNA
<213>  Drosophila suzukii


<220>
<221>  misc_feature
<222>  (135)..(135)
<223>  n is a, c, g, or t

<400>  4
ctgctgaaac catcggcgta aacctctaat taaggctagt aacctttgta gaaaattatt       60

tagtttatat ttttaaacat aaattatttt tgaaattgta actaaaaatg tatgcctatt      120

ttaaaaattc cttcntaaga aaattagttt aagtagtaca cttttgacgc tcactgtatc      180

aaaatttttc tggagcgcca tctggggagc ttactcaatt tcaaaagctt tactttactt      240

aggtaaaggg ccgaagataa aagctgacgt aggattttaa ctgaatggga gctttctaag      300

gttgtttatg cactggagag atagtagata aatgcacttc ccacagaacc cagagctttc      360

ggatctgaag gtcagttggg acttggaccc acaagtcgag cttagtttaa tagtcagcgc      420

gcttaaacga cgacaactgc acggcgcgcc cccattaata atatatattt ttccaaaaat      480

aaacttaaaa aaaataatta aataaaaaat aatattaaca ttgaacgcgc ggtgcacgat      540

tttttgacaa caattcagtt tcgcttttcc ttagatttcc atataatttt ttcctttgtg      600

ttttccacac acgtggtgtt cttctgttac tcgcacttcg gtagtggtta ttttttttgg      660

gttacattga aaaaagtaca tcgactgcca gccgaattcc cattcgaacc ttaatttgac      720

caattgacgg aaaattaatt gcgcacaaaa ttaattagca agcgaatata tatttttttt      780

tttacgtgaa acgagcgtgt tatttgtcaa aaagatttta caactgattg tgtttaagta      840

aattaacctg agatatatct atgtgtttgt gctacgatcc gaaattccaa tattgcggta      900

agtggtgtga tttttggcgg gaataaaatc ggtttccctt tgcgtcgtat ttctctcccg      960

atttttctgg cgccaaaatg aatgaaaacg gttggcaaca ttttcaactt aacatggcaa     1020

catgcgaatc taaccgctgt taggggaact tgtaagggaa catgttagaa attctggaac     1080

acctctccaa ctttgttttg tttggcgctc tctctttgaa atgtagaatc tcttattttg     1140

gaacgggagc ggttcattga cccagcaaca acaatattcg ccatatcttc tccttcccag     1200

cgatttgcag aattcatccg tactacagtt agaaataatt ctagtccaac agtaagcaaa     1260

gtcgatcggc gaaatcggga gccgcgttca gttgccttcg agccgtactt agaagtggta     1320

gagcccccag gaatcggaga gattatcccg taacttgata ctctgacgaa atcccccaga     1380

cagacccctc gcaatcacac acagctatat actgatatat agatctatat atattgcgca     1440

gatcaccctt taaatgcctt tcgtccagag attccaacaa atacgtaatt ttgttatgaa     1500

cgtctgcctc gtcgaggcca tccaattcgt gcaggacctc acgcatgccc tctgcctgga     1560

cctccagggc ctgttcaacc tcaagtagag attcccgagc gatctcccag cggtaagtca     1620

cgccatttgg ccgcgggccg cgaaaagtga aagtgcggga gaaattacgg ttatcgggtt     1680

ttcgggagaa aggggaaagt ggaaacctca tggcagcagc cctccatttt gagaggttat     1740

gtcaaaggaa aggaagagaa aggaagttaa aaaatgttag caaggaaaat attagccttc     1800

gtttttcata agtacaccaa taaaccacat tttactgggt gtcaaaagtt agagaattaa     1860

acagcaagtt accaaaatta gtttgaaggc aaaactttag ctagttacgt ttttagaggg     1920

ttctggaatt acaaatgccc acagcaggag aaatataaat ttgtgttttc attttaaaca     1980

gattatgttt taaaaagtaa tacatcaaaa ttgactttag ccgtttttta aataggtaat     2040

ttggtatata aacatacatt ttttaataaa aatatgaaca ctttgttttt atttttgtac     2100

tggtaggcaa actacaagat ataactgtta aataaaaaaa gattttaagt gtaggcaata     2160

aaaaatcaaa tcaaaaatat attttgaata aaagtgaagg tcaaaaatga aagacttcaa     2220

agtaccactc tactcatgaa gctataataa taatgggtaa ataaacagta tattacatgt     2280

tcgttttaaa tattttcaat gcaaaaatgc cctgcttata ttaagcttta ctttcaccac     2340

aactgttccc atcatctatt tttaatatta ttgacctcgg acttcttaca tttcatcgcc     2400

agctcatccc atcaatcacc cagataaact accaccttca ttaacacacc gcaagcatct     2460

cacaacgctg accaataaac gcctcgacat ccagaatccc caactgagac ggacgttttg     2520

cttggttttt aaaactaaac gaccctttgg tagccgcacc tattgacctt tcacacattc     2580

atatccctcg gtccacaagc cgacccactg atccattcat tcacctccca cg             2632


<210>  5
<211>  3267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Antidote transgene sequence including bottleneck promoter, myd88 
       coding sequence, and SV40 3' UTR

<400>  5
atgaacccta ttaagatccc gacacctctt ttcgcctttc tgcataataa tttcgccatt       60

ccaccgctgt ataattgcgt attcgccttc agtaaatgaa aattgcagtt gtattttata      120

atttcgtttt tcattttccc tctctcattt tctacgtctg tttggccagc tgtcaattgc      180

cgagtgtcga ctgaattatt ggccattaat tgcattgatt ttgattgtaa tgaaccagct      240

aatgaaacgt caacagctaa gagtgggcca taaaacccgg aggaattatc ttctagaaac      300

cttgaacatc tgtcgcgaat ttaagtttta agctgtttat gaattagtac cctcgctaat      360

ccttttcgaa aggaccttat aaaagtgcgc ggattagcaa aaagatttgt gtaaaattgg      420

tgcgcggaaa acacgcaaaa ttcgcgtgct cgggttgcaa ttaccgggaa atgtgggcct      480

tttttacttg cagggcttag gtagtttccc ggaatggagt caagctaagc tagccaggta      540

cctgagttcc ccggaccagc tgtccggtgc tgaaactaga gcaggtagtc cccagaatcc      600

cagctatata aggcctgcct tcctggcaac agcatcacat tcgttcatca gccttcaacc      660

ctaggatgcg ccctcgattt gtatgccatc agcagcactc ggtggcccat tcccacttcc      720

atcccctgtc tcacttccag acccattccc atacccatac ccacttccag cgccatccca      780

atccccatcc ccatccccat cacttttacg acgccactga cgtcggctat cggcgttatc      840

gcaccgccaa catggtggtg gccgagggag ttatggactc cggatcgggg atcggtacgg      900

gaatgggaat ggggcacttc aacgagaccc ccttatccga gctgggcgtg gagacccgct      960

cgcagctgtc ccgcatgctg aaccgcaaga aggtgctgcg ctccgaggag ggctaccagc     1020

gggactggcg gggcatctcg gagctggcca agcagaaggg attcgtcgac gagaacgcca     1080

acaatcccat ggatctggtg ctgatcagct ggagccagcg gagcccgcaa accgccaagg     1140

tgggccatct cgagaacttc ctgggcatca tcgatcggtg ggatgtctgc gacgatatcc     1200

aggagaatct ggccaaggac acccggcgct tcatcataaa gcaggagcag cggcagacct     1260

ctctggtgga ggcgtgtccc ccgcccccca gcgactgttt cgagaccaac aacaactaca     1320

gcagcaacaa caacatcaca gtgggccaaa gtgtccagat cctgagcgac gaggatcaga     1380

gatgtgtgca gatgggccaa ccgctgccca gatacaatgc ctgtgtcttg tacgcagagg     1440

cagacatcga tcatgccacc gagatcatga ataacctaga gtctgagcga tacaatctca     1500

ggcttttcct gcgccatcgc gacatgctaa tgggcgtacc cttcgagcat gtccaactct     1560

cccacttcat ggccacccgc tgtaatcacc tgatcgtcgt gctcacagag gagtttcttc     1620

ggagtccgga gaacacgtac ctcgtgaact tcacccagaa gatacaaatc gagaaccaca     1680

ctcgcaagat cataccgatt ctgtacaagc cagacatgca cataccccag accctgggca     1740

tctatacgca catcaagtac gccggggact ccaagttgtt caacttctgg gacaagttgg     1800

ctcgatcgct gcacgatctg gatgcctgtt ccatttactc cacgcgccag gtgcaaacac     1860

cctcgccagt ggaggaatcg actccccagc gggtgaccac gcccagcatt cggatacaga     1920

tcaacgacaa ggatgtgacc gacatgccca acttcaaggt gccggaggcg gagaccacca     1980

tcgtttcggt atccggcgat accggttccc ctctgccgga acacaagccg aagaaaaagg     2040

atcgctttct gcgaagaatc acgcacagtt tcgccaagac accgaagaat gaggggagca     2100

gtgcgaagac cctgcgacac gcgcactccg tcagcaccat aaatgtcacg gaacgggaga     2160

ggacactcag tgccagcagc tccaatatct ccaccacctc ggagagcaag aagagcttca     2220

tcaagtggca gccgaatatc ctgaagaagg ccctattctc ccgatccagc aacaagctac     2280

agacgccggg ttgacatatg gatctttgtg aaggaacctt acttctgtgg tgtgacataa     2340

ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta     2400

taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact     2460

gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa     2520

atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag     2580

aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat     2640

gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct     2700

gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat     2760

cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct     2820

ccccctgaac ctgaaacata aaatgaatgg aattgttgtt gttaacttgt ttattgcagc     2880

ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     2940

actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggttcca     3000

agcgctccgt acgcgtatcg ataagcttta agatacattg atgagtttgg acaaaccaca     3060

actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt     3120

gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt     3180

caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt     3240

atggctgatt atgatctaga gtcgcgg                                         3267


