                         SEQUENCE LISTING

<110>  MeiraGTx UK II Limited
       MeiraGTx, LLC
       Volles, Michael J.
       Danos, Olivier F.
       Boyne, Alex K.
       Zennou, Veronique
       Guo, Xuecui
 
<120>  REGULATION OF GENE EXPRESSION VIA APTAMER-MEDIATED RNA CLEAVAGE

<130>  162027.46376

<140>  PCT/US17/16303
<141>  2017-02-02

<150>  U.S. 62/290,187
<151>  2016-02-02

<150>  U.S. 62/290,209
<151>  2016-02-02

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp-twister in pEGFP-C1

<400>  1
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac cgcggcttgt       60

aatgcggccg tgtaaataat ttacacgtcg gtctcaagcc cgataaacgc agagagcaag      120

gcggccgcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc      180

ccctgaacct gaaacataaa atgaatgc                                         208


<210>  2
<211>  217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  es-twister in pEGFPC1

<400>  2
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac cgcggcaata       60

aagcggttac aagcccgcaa aaatagcaga gtaatgtcgc gatagcgcgg cattaatgca      120

gctttattgg cggccgccat accacatttg tagaggtttt acttgcttta aaaaacctcc      180

cacacctccc cctgaacctg aaacataaaa tgaatgc                               217


<210>  3
<211>  208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp-twister-mut in pEGFP-C1

<400>  3
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac cgcggcttgt       60

gatgcggccg tgtaaataat ttacacgtcg gtctcaagcc cgataaacgc agagagcaag      120

gcggccgcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc      180

ccctgaacct gaaacataaa atgaatgc                                         208


<210>  4
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp_no Sac 0HP in pEGFP-C1

<400>  4
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac ttgtaatgcg       60

gccgtgtaaa taatttacac gtcggtctca agcccgataa acgcagagag caaggcggcc      120

gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga      180

acctgaaaca taaaatgaat gc                                               202


<210>  5
<211>  205
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp_no Sac 3HP in pEGFP-C1

<400>  5
gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaac gccttgtaat       60

gcggccgtgt aaataattta cacgtcggtc tcaagcccga taaacgcaga gagcaaggcg      120

gccgccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc      180

tgaacctgaa acataaaatg aatgc                                            205


<210>  6
<211>  203
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp_No Sac 0HP in pLuc

<400>  6
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acttgtaatg       60

cggccgtgta aataatttac acgtcggtct caagcccgat aaacgcagag agcaaggcgg      120

ccgccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct      180

gaacctgaaa cataaaatga atg                                              203


<210>  7
<211>  207
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wasp_No Sac 3HP in pLuc

<400>  7
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtaaataatt tacacgtcgg tctcaagccc gataaacgca gagagcaagg      120

cggccgccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc      180

cctgaacctg aaacataaaa tgaatgc                                          207


<210>  8
<211>  275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua1 in pLuc

<400>  8
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtaaatcact catataatcg cgtggatatg gcacgcaagt ttctaccggg      120

caccgtaaat gtccgactat gggtgattta cacgtcggtc tcaagcccga taaacgcaga      180

gagcaaggcg gccgccatac cacatttgta gaggttttac ttgctttaaa aaacctccca      240

cacctccccc tgaacctgaa acataaaatg aatgc                                 275


<210>  9
<211>  271
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua2 in pLuc

<400>  9
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtaaaactca tataatcgcg tggatatggc acgcaagttt ctaccgggca      120

ccgtaaatgt ccgactatgg gttttacacg tcggtctcaa gcccgataaa cgcagagagc      180

aaggcggccg ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc      240

tccccctgaa cctgaaacat aaaatgaatg c                                     271


<210>  10
<211>  267
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua3 in pLuc

<400>  10
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtaactcata taatcgcgtg gatatggcac gcaagtttct accgggcacc      120

gtaaatgtcc gactatgggt tacacgtcgg tctcaagccc gataaacgca gagagcaagg      180

cggccgccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc      240

cctgaacctg aaacataaaa tgaatgc                                          267


<210>  11
<211>  263
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua4 in pLuc

<400>  11
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtatcatata atcgcgtgga tatggcacgc aagtttctac cgggcaccgt      120

aaatgtccga ctatggtaca cgtcggtctc aagcccgata aacgcagaga gcaaggcggc      180

cgccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg      240

aacctgaaac ataaaatgaa tgc                                              263


<210>  12
<211>  261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua5 in pLuc

<400>  12
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gttcatataa tcgcgtggat atggcacgca agtttctacc gggcaccgta      120

aatgtccgac tatggacacg tcggtctcaa gcccgataaa cgcagagagc aaggcggccg      180

ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa      240

cctgaaacat aaaatgaatg c                                                261


<210>  13
<211>  259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua6 in pLuc

<400>  13
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtcatataat cgcgtggata tggcacgcaa gtttctaccg ggcaccgtaa      120

atgtccgact atgacacgtc ggtctcaagc ccgataaacg cagagagcaa ggcggccgcc      180

ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc      240

tgaaacataa aatgaatgc                                                   259


<210>  14
<211>  257
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua7 in pLuc

<400>  14
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gcatataatc gcgtggatat ggcacgcaag tttctaccgg gcaccgtaaa      120

tgtccgacta tgcacgtcgg tctcaagccc gataaacgca gagagcaagg cggccgccat      180

accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg      240

aaacataaaa tgaatgc                                                     257


<210>  15
<211>  255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua8 in pLuc

<400>  15
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gatataatcg cgtggatatg gcacgcaagt ttctaccggg caccgtaaat      120

gtccgactat cacgtcggtc tcaagcccga taaacgcaga gagcaaggcg gccgccatac      180

cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa      240

acataaaatg aatgc                                                       255


<210>  16
<211>  253
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua9 in pLuc

<400>  16
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt atataatcgc gtggatatgg cacgcaagtt tctaccgggc accgtaaatg      120

tccgactata cgtcggtctc aagcccgata aacgcagaga gcaaggcggc cgccatacca      180

catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac      240

ataaaatgaa tgc                                                         253


<210>  17
<211>  247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua12 in pLuc

<400>  17
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccga taatcgcgtg gatatggcac gcaagtttct accgggcacc gtaaatgtcc      120

gactcgtcgg tctcaagccc gataaacgca gagagcaagg cggccgccat accacatttg      180

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa      240

tgaatgc                                                                247


<210>  18
<211>  245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua13 in pLuc

<400>  18
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccat aatcgcgtgg atatggcacg caagtttcta ccgggcaccg taaatgtccg      120

actgtcggtc tcaagcccga taaacgcaga gagcaaggcg gccgccatac cacatttgta      180

gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg      240

aatgc                                                                  245


<210>  19
<211>  243
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua14 in pLuc

<400>  19
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccta atcgcgtgga tatggcacgc aagtttctac cgggcaccgt aaatgtccga      120

cgtcggtctc aagcccgata aacgcagaga gcaaggcggc cgccatacca catttgtaga      180

ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa      240

tgc                                                                    243


<210>  20
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua15 in pLuc

<400>  20
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccat ataatcgcgt ggatatggca cgcaagtttc taccgggcac cgtaaatgtc      120

cgactatgtc ggtctcaagc ccgataaacg cagagagcaa ggcggccgcc ataccacatt      180

tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa      240

aatgaatgc                                                              249


<210>  21
<211>  247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua16 in pLuc

<400>  21
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggcata taatcgcgtg gatatggcac gcaagtttct accgggcacc gtaaatgtcc      120

gactattcgg tctcaagccc gataaacgca gagagcaagg cggccgccat accacatttg      180

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa      240

tgaatgc                                                                247


<210>  22
<211>  245
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua17 in pLuc

<400>  22
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggctat aatcgcgtgg atatggcacg caagtttcta ccgggcaccg taaatgtccg      120

actatcggtc tcaagcccga taaacgcaga gagcaaggcg gccgccatac cacatttgta      180

gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg      240

aatgc                                                                  245


<210>  23
<211>  253
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua18 in pLuc

<400>  23
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtataatcgc gtggatatgg cacgcaagtt tctaccgggc accgtaaatg      120

tccgactaca cgtcggtctc aagcccgata aacgcagaga gcaaggcggc cgccatacca      180

catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac      240

ataaaatgaa tgc                                                         253


<210>  24
<211>  251
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua19 in pLuc

<400>  24
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt tataatcgcg tggatatggc acgcaagttt ctaccgggca ccgtaaatgt      120

ccgactaacg tcggtctcaa gcccgataaa cgcagagagc aaggcggccg ccataccaca      180

tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat      240

aaaatgaatg c                                                           251


<210>  25
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  waspGua20 in pLuc

<400>  25
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt ataatcgcgt ggatatggca cgcaagtttc taccgggcac cgtaaatgtc      120

cgactacgtc ggtctcaagc ccgataaacg cagagagcaa ggcggccgcc ataccacatt      180

tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa      240

aatgaatgc                                                              249


<210>  26
<211>  296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Epo-waspGua16

<400>  26
gctgtacacg ggagaggtct gcaggagagg ggacaggtga ggatccacgc cttgtaatgc       60

ggcatataat cgcgtggata tggcacgcaa gtttctaccg ggcaccgtaa atgtccgact      120

attcggtctc aagcccgata aacgcagaga gcaaggcgct cgagggccca gatctaattc      180

accccaccag tgcaggctgc ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc      240

cacaagtatc actaagctcg ctttcttgct gtccaatttc tattaaaggt tccttt          296


<210>  27
<211>  296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Epo-mutwaspGua16

<400>  27
gctgtacacg ggagaggtct gcaggagagg ggacaggtga ggatccacgc cttgtgatgc       60

ggcatataat cgcgtggata tggcacgcaa gtttctaccg ggcaccgtaa atgtccgact      120

attcggtctc aagcccgata aacgcagaga gcaaggcgct cgagggccca gatctaattc      180

accccaccag tgcaggctgc ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc      240

cacaagtatc actaagctcg ctttcttgct gtccaatttc tattaaaggt tccttt          296


<210>  28
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  twister_theo_1 in pLuc

<400>  28
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggcatg ataccagccg aaaggccctt ggcagcattc ggtctcaagc ccgataaacg      120

cagagagcaa ggcggccgcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct      180

cccacacctc cccctgaacc tgaaacataa aatgaatgc                             219


<210>  29
<211>  221
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  twister_theo_2 in pLuc

<400>  29
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggcggc gataccagcc gaaaggccct tggcagcgtc tcggtctcaa gcccgataaa      120

cgcagagagc aaggcggccg ccataccaca tttgtagagg ttttacttgc tttaaaaaac      180

ctcccacacc tccccctgaa cctgaaacat aaaatgaatg c                          221


<210>  30
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  twister_theo_3 in pLuc

<400>  30
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggcgcg ataccagccg aaaggccctt ggcagcgttc ggtctcaagc ccgataaacg      120

cagagagcaa ggcggccgcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct      180

cccacacctc cccctgaacc tgaaacataa aatgaatgc                             219


<210>  31
<211>  219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  twister_theo_4 in pLuc

<400>  31
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggcctg ataccagccg aaaggccctt ggcagcagtc ggtctcaagc ccgataaacg      120

cagagagcaa ggcggccgcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct      180

cccacacctc cccctgaacc tgaaacataa aatgaatgc                             219


<210>  32
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG_wtWaspGua16 in pLuc

<400>  32
atgacgcctt gtaatgcggc atataatcgc gtggatatgg cacgcaagtt tctaccgggc       60

accgtaaatg tccgactatt cggtctcaag cccgataaac gcagagagca aggcgaaggc      120

ggatcttcag gtagtggcga gggcagagga agtcttctaa catgcggtga cgtggaggag      180

aatcccggcc ccgaagacgc caaaaacata aagaaaggcc cggcgcca                   228


<210>  33
<211>  228
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ATG_mutWaspGua16 in pLuc

<400>  33
atgacgcctt gtgatgcggc atataatcgc gtggatatgg cacgcaagtt tctaccgggc       60

accgtaaatg tccgactatt cggtctcaag cccgataaac gcagagagca aggcgaaggc      120

ggatcttcag gtagtggcga gggcagagga agtcttctaa catgcggtga cgtggaggag      180

aatcccggcc ccgaagacgc caaaaacata aagaaaggcc cggcgcca                   228


<210>  34
<211>  255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  10aa_wtWaspGua16 in pLuc

<400>  34
atggtgagca agggcgagga gctgttcacc acgccttgta atgcggcata taatcgcgtg       60

gatatggcac gcaagtttct accgggcacc gtaaatgtcc gactattcgg tctcaagccc      120

gataaacgca gagagcaagg cgaaggcgga tcttcaggta gtggcgaggg cagaggaagt      180

cttctaacat gcggtgacgt ggaggagaat cccggccccg aagacgccaa aaacataaag      240

aaaggcccgg cgcca                                                       255


<210>  35
<211>  255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  10aa_mutWaspGua16 in pLuc

<400>  35
atggtgagca agggcgagga gctgttcacc acgccttgtg atgcggcata taatcgcgtg       60

gatatggcac gcaagtttct accgggcacc gtaaatgtcc gactattcgg tctcaagccc      120

gataaacgca gagagcaagg cgaaggcgga tcttcaggta gtggcgaggg cagaggaagt      180

cttctaacat gcggtgacgt ggaggagaat cccggccccg aagacgccaa aaacataaag      240

aaaggcccgg cgcca                                                       255


<210>  36
<211>  271
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GT_8bp in pLuc

<400>  36
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa taaaggcgta       60

taatcgcgtg gatatggcac gcaagtttct accgggcacc gtaaatgtcc gactacgcct      120

tgtaatgcgg ccgtgtaaat aatttacacg tcggtctcaa gcccgataaa cgcagagagc      180

aaggcggccg ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc      240

tccccctgaa cctgaaacat aaaatgaatg c                                     271


<210>  37
<211>  265
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TG_9b in pLuc

<400>  37
tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa acgccttgta       60

atgcggccgt gtaaataatt tacacgtcgg tctcaagccc gataaacgca gagagcaagg      120

cgtataatcg cgtggatatg gcacgcaagt ttctaccggg caccgtaaat gtccgactac      180

gccttgatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc      240

tgaacctgaa acataaaatg aatgc                                            265


<210>  38
<211>  65
<212>  DNA
<213>  Nasonia vitripennis

<400>  38
cttgtaatgc ggccgtgtaa ataatttaca cgtcggtctc aagcccgata aacgcagaga       60

gcaag                                                                   65


<210>  39
<211>  76
<212>  DNA
<213>  Unknown

<220>
<223>  Twister ribozyme from an environmental sample

<400>  39
ggcaataaag cggttacaag cccgcaaaaa tagcagagta atgtcgcgat agcgcggcat       60

taatgcagct ttattg                                                       76


