                         SEQUENCE LISTING

<110>  DA VOLTERRA et al.
 
<120>  BETA-LACTAMASE VARIANTS

<130>  B2185PC00

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  wild-type VIM-2

<400>  1

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Gln Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Gln Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  2
<211>  26
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 signal peptide

<400>  2

Met Phe Lys Leu Leu Ser Lys Leu Leu Val Tyr Leu Thr Ala Ser Ile 
1               5                   10                  15      


Met Ala Ile Ala Ser Pro Leu Ala Phe Ser 
            20                  25      


<210>  3
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  3

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  4
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  4

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  5
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  5

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Gln Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  6
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  6

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  7
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  7

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  8
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  8

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  9
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  9

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  10
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  10

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Gln Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  11
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  11

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  12
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  12

Val Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  13
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  13

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  14
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  14

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  15
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  15

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Gln Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  16
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  16

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  17
<211>  240
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  17

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn Arg Ser Val Val Glu 
225                 230                 235                 240 


<210>  18
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  18

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  19
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  19

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Glu Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  20
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  20

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Gln Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  21
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  21

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr Asn Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


<210>  22
<211>  235
<212>  PRT
<213>  artificial

<220>
<223>  VIM-2 variant

<400>  22

Met Asp Ser Ser Gly Glu Tyr Pro Thr Val Ser Glu Ile Pro Val Gly 
1               5                   10                  15      


Glu Val Arg Leu Tyr His Ile Ala Asp Gly Val Trp Ser His Ile Ala 
            20                  25                  30          


Thr Arg Ser Phe Asp Gly Ala Val Tyr Pro Ser Asn Gly Leu Ile Val 
        35                  40                  45              


Arg Asp Gly Asp Glu Leu Leu Leu Ile Asp Thr Ala Trp Gly Ala Lys 
    50                  55                  60                  


Asn Thr Ala Ala Leu Leu Ala Glu Ile Glu Lys Gln Ile Gly Leu Pro 
65                  70                  75                  80  


Val Thr Arg Ala Val Ser Thr His Phe His Asp Asp Arg Val Gly Gly 
                85                  90                  95      


Val Asp Val Leu Arg Ala Ala Gly Val Ala Thr Tyr Ala Ser Pro Ser 
            100                 105                 110         


Thr Arg Arg Leu Ala Glu Val Glu Gly Asn Glu Ile Pro Thr His Ser 
        115                 120                 125             


Leu Asp Gly Leu Ser Ser Ser Gly Asp Ala Val Arg Phe Gly Pro Val 
    130                 135                 140                 


Glu Leu Phe Tyr Pro Gly Ala Ala His Ser Thr Asp Asn Leu Val Val 
145                 150                 155                 160 


Tyr Val Pro Ser Ala Ser Val Leu Tyr Gly Gly Cys Ala Ile Tyr Glu 
                165                 170                 175     


Leu Ser Arg Thr Ser Ala Gly Asn Val Ala Asp Ala Asp Leu Ala Glu 
            180                 185                 190         


Trp Pro Thr Ser Ile Glu Arg Ile Gln Gln His Tyr Pro Glu Ala Gln 
        195                 200                 205             


Phe Val Ile Pro Gly His Gly Leu Pro Gly Gly Leu Asp Leu Leu Lys 
    210                 215                 220                 


His Thr Thr Asn Val Val Lys Ala His Thr Asn 
225                 230                 235 


