
                                SEQUENCE LISTING

<110> Blain, Stacy W.

<120> P27 Tyrosine Phosphorylation as a Marker
  of Cdk4 Activity and Methods of Use Thereof
  

<130> 5168-P06443WO00

<140> PCT/US2017/019184   
<141> 2017-02-23  

<150> 62/298,584          
<151> 2016-02-23  

<160> 18

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 198
<212> PRT
<213> Homo sapiens

<400> 1
Met Ser Asn Val Arg Val Ser Asn Gly Ser Pro Ser Leu Glu Arg Met
 1               5                  10                  15      
Asp Ala Arg Gln Ala Glu His Pro Lys Pro Ser Ala Cys Arg Asn Leu
            20                  25                  30          
Phe Gly Pro Val Asp His Glu Glu Leu Thr Arg Asp Leu Glu Lys His
        35                  40                  45              
Cys Arg Asp Met Glu Glu Ala Ser Gln Arg Lys Trp Asn Phe Asp Phe
    50                  55                  60                  
Gln Asn His Lys Pro Leu Glu Gly Lys Tyr Glu Trp Gln Glu Val Glu
65                  70                  75                  80  
Lys Gly Ser Leu Pro Glu Phe Tyr Tyr Arg Pro Pro Arg Pro Pro Lys
                85                  90                  95      
Gly Ala Cys Lys Val Pro Ala Gln Glu Ser Gln Asp Val Ser Gly Ser
            100                 105                 110         
Arg Pro Ala Ala Pro Leu Ile Gly Ala Pro Ala Asn Ser Glu Asp Thr
        115                 120                 125             
His Leu Val Asp Pro Lys Thr Asp Pro Ser Asp Ser Gln Thr Gly Leu
    130                 135                 140                 
Ala Glu Gln Cys Ala Gly Ile Arg Lys Arg Pro Ala Thr Asp Asp Ser
145                 150                 155                 160 
Ser Thr Gln Asn Lys Arg Ala Asn Arg Thr Glu Glu Asn Val Ser Asp
                165                 170                 175     
Gly Ser Pro Asn Ala Gly Ser Val Glu Gln Thr Pro Lys Lys Pro Gly
            180                 185                 190         
Leu Arg Arg Arg Gln Thr
        195             


<210> 2
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> K1 site

<400> 2
Pro Glu Phe Tyr Tyr Arg Pro Pro Arg Pro Pro Lys Gly Ala Cys Lys
 1               5                  10                  15      
Val Pro
        


<210> 3
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> K2 site

<400> 3
Asp Val Ser Gly Ser Arg Pro Ala Ala Pro Leu Ile Gly Ala Pro Ala
 1               5                  10                  15      


<210> 4
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> K3 site

<400> 4
Gly Ser Val Glu Gln Thr Pro Lys Lys Pro Gly Leu Arg Arg Arg Gln
 1               5                  10                  15      


<210> 5
<211> 59
<212> PRT
<213> Artificial Sequence

<220> 
<223> Brk SH3

<400> 5
Lys Tyr Val Gly Leu Trp Asp Phe Lys Ser Arg Thr Asp Glu Glu Leu
 1               5                  10                  15      
Ser Phe Arg Ala Gly Asp Val Phe His Val Ala Arg Lys Glu Glu Gln
            20                  25                  30          
Trp Trp Trp Ala Thr Leu Leu Asp Glu Ala Gly Gly Ala Val Ala Gln
        35                  40                  45              
Gly Tyr Val Pro His Asn Tyr Leu Ala Glu Arg
    50                  55                  


<210> 6
<211> 56
<212> PRT
<213> Artificial Sequence

<220> 
<223> Src SH3

<400> 6
Thr Phe Val Ala Leu Tyr Asp Tyr Glu Ser Arg Thr Glu Thr Asp Leu
 1               5                  10                  15      
Ser Phe Lys Lys Gly Glu Arg Leu Gln Ile Val Asn Asn Thr Glu Gly
            20                  25                  30          
Asp Trp Trp Leu Ala His Ser Leu Thr Thr Gly Gln Thr Gly Tyr Ile
        35                  40                  45              
Pro Ser Asn Tyr Val Ala Pro Ser
    50                  55      


<210> 7
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence

<400> 7
Pro Pro Pro Pro
 1              


<210> 8
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence

<400> 8
Ala Ala Ala Ala
 1              


<210> 9
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence

<400> 9
Pro Lys Lys Pro
 1              


<210> 10
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> Forward primer

<400> 10
ggcctcgagc tagctctcct gcgccg                                      26

<210> 11
<211> 65
<212> DNA
<213> Artificial Sequence

<220> 
<223> Reverse primer

<400> 11
ggggtctaga gccaccatgg actacaagga cgacgatgac aagcgcaagt ggaatttcga 60
ttttc                                                             65

<210> 12
<211> 134
<212> PRT
<213> Artificial Sequence

<220> 
<223> Alt-Brk

<400> 12
Met Val Ser Arg Asp Gln Ala His Leu Gly Pro Lys Tyr Val Gly Leu
 1               5                  10                  15      
Trp Asp Phe Lys Ser Arg Thr Asp Glu Glu Leu Ser Phe Arg Ala Gly
            20                  25                  30          
Asp Val Phe His Val Ala Arg Lys Glu Glu Gln Trp Trp Trp Ala Thr
        35                  40                  45              
Leu Leu Asp Glu Ala Gly Gly Ala Val Ala Gln Gly Tyr Val Pro His
    50                  55                  60                  
Asn Tyr Leu Ala Glu Arg Glu Thr Val Glu Ser Glu Pro Ala Gly His
65                  70                  75                  80  
Ala Gly Cys Ala Ala Leu Gln Asp Leu Ala Ala Cys Arg Gly Pro Ala
                85                  90                  95      
Ala Pro Glu Arg Gly Gly Val Leu Pro Gln Pro Ala Arg Ala Cys Glu
            100                 105                 110         
Leu Pro Gln Gly Pro Glu Pro Val Pro Arg Pro Ala Ala Gly Arg Ala
        115                 120                 125             
Leu Pro Glu Ala Arg Ala
    130                 


<210> 13
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> Actin forward primer

<400> 13
aaaatctggc accacacctt ctac                                        24

<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> Actin reverse primer

<400> 14
tagcacagcc tggatagcaa cg                                          22

<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Brk forward primer

<400> 15
ccaagtatgt gggcctctgg                                             20

<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Brk reverse primer

<400> 16
aaagaaccac ggttccgact                                             20

<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Alt-Brk forward primer

<400> 17
gacggtggag tcggaacctg                                             20

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> Alt-Brk reverse primer

<400> 18
tagttcacaa gctcgggcag                                             20
