                               SEQUENCE LISTING

<110> WEISSLEDER, RALPH
      LEE, HAKHO
      PARK, KISOO
      HUANG, CHEN-HAN
 
<120> POLARIZATION-BASED FLUORESCENT NUCLEIC ACID DETECTION

<130> 29539-0269WO1

<140> PCT/US2017/024328
<141> 2017-03-27

<150> 62/313,427
<151> 2016-03-25

<160> 95    

<170> PatentIn version 3.5

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
caagacgggc gggtgtggta ggcgcccgtg                                        30


<210> 2
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
acttgatggc gggtgtggta ggcgccatct                                        30


<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
acttgatggc gggtttggta ggcgccgtcg                                        30


<210> 4
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
actggatggc gggtttggta ggcgccatct                                        30


<210> 5
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
acatgatggc gggtgtggta ggcgtcgtct                                        30


<210> 6
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
acatgatggc gggtttggta ggcgccgtct                                        30


<210> 7
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
caagacgggc gggtttggta ggcgcccgag                                        30


<210> 8
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
caagacgggc gggtttggga ggcgcccgag                                        30


<210> 9
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
acttgatggc gggtttggta ggcgccctct                                        30


<210> 10
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
caagacgggc gggtttggta ggcgccgtct                                        30


<210> 11
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
acttgatggc gggtttggta ggcgccccag                                        30


<210> 12
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
acttgatggt gggtttggta ggcgccgtct                                        30


<210> 13
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
acagacgggc gggtttggta ggcgccgact                                        30


<210> 14
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
cctgacgggc gggtttggta ggcgccgtct                                        30


<210> 15
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
acagacgggc gggttcggta ggcgccctag                                        30


<210> 16
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
caatgtacag tattgtggag catgtggttt aattcga                                37


<210> 17
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
caatgtacag tattgtggag gaagggagta aagttaat                               38


<210> 18
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
caatgtacag tattgtggag gcaaggcgat aaggt                                  35


<210> 19
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
caatgtacag tattgttcta gttaatacct agggatagtg                             40


<210> 20
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
caatgtacag tattgtggag gaagggcagt aagttaa                                37


<210> 21
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
caatgtacag tattgtggga agaacatatg tgtaagtaac                             40


<210> 22
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
caatgtacag tattgttgct tcaggaccat atttctctac                             40


<210> 23
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
caatgtacag tattgtccag aagcaaggtt tagaattg                               38


<210> 24
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
caatgtacag tattgtctgc tatccaccct caaacag                                37


<210> 25
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 25
caatgtacag tattgtcggt aggttattct tatggtggag                             40


<210> 26
<211> 68
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 26
gaagaacgag tgtgagagtg gaaagttcac actgtgacgg tatcttacca gaaagggacg       60

gctaacta                                                                68


<210> 27
<211> 68
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
gaagaacaag gacgttagta actgaacgtc ccctgacggt atctaaccag aaagccacgg       60

ctaactac                                                                68


<210> 28
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
gtcttcaggg acgataatga cggtacctga ggaggaagcc acggctaact acgtgccagc       60

agccgcggta at                                                           72


<210> 29
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
ggaggcagca gtagggaatc ttccacaatg gacgcaagtc tgatggagca a                51


<210> 30
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
cctttcgcaa ccgacgaagc ttttgtgacg gtaggttgag aagaagcacc ggctaact         58


<210> 31
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 31
gttgttaggg aagaacaagt gcgttcaaat agggcggcac cttgacggta cctaaccaga       60

aagccacgg                                                               69


<210> 32
<211> 62
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 32
tcatggcatg cggccttgcg gtcccgcact ttcatcttcc gattctacgc ggtattagcg       60

ac                                                                      62


<210> 33
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (29)..(29)
<223> a, c, t, g, unknown or other

<400> 33
ggaggaaggt gataaggtta atacccttnt caattgacgt tacccgcaga agaagcaccg       60

gctaactcc                                                               69


<210> 34
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 34
acggagttag ccggtgcttc ttctgcgagt aacgtcaatt gctgcggtta ttaaccacaa       60

caccttcctc c                                                            71


<210> 35
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
tcagcgggga ggaaggtggt gaacttaata cgttcatcaa ttgacgttac tcgcagaaga       60

agcaccggct aa                                                           72


<210> 36
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 36
aatgcgtaga gatcaggagg aacatccatg gcgaaggcag ctacctggac caacattga        59


<210> 37
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
ttcagtgggg aggaggattg ataggttaag agctgattga ttggacgtta cccacagaag       60

aagcaccggc t                                                            71


<210> 38
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 38
tttgtcaggg aagaaaaggc tgttgctaat atcagcggct gatgacggta cctgaagaat       60

aagcaccggc ta                                                           72


<210> 39
<211> 65
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 39
ttaagtgggg aggaaaagct tgtggttaat acccacaagc cctgacgtta cccacagaat       60

aagca                                                                   65


<210> 40
<211> 72
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 40
tcattagaca taaagtgcag tcatgtcatg tcatactgtt ttgtcatgtc ataatatgaa       60

taaggatcgg ct                                                           72


<210> 41
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 41
tctttcacca tcgacgaagg tccgggttct ctggattgac ggtaggtgga gaagaagcac       60

cggccaa                                                                 67


<210> 42
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
taccgaccag cgggtttatg atatctgcct gaaatacggc tttgattcgc agcagac          57


<210> 43
<211> 57
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
cggcatagtc atttgccgtg ccataccctc cgcaggttcc ggttttgtct ccgactg          57


<210> 44
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 44
cgcattggca taagtcgcaa tccccgccgc atgcagcgcg tccataccgc ccatcttgt        59


<210> 45
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
acccaacctg ttaaccaacc tacttgaggg gttacagcca ttccccagcc gctttttg         58


<210> 46
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
tgaatattta gcttgtacct taccggattt tttcaaaagt tcatttgtta attcagat         58


<210> 47
<211> 61
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 47
tagccgaggt agaggggaac gagattccca cgcactctct agaaggactc tcatcgagcg       60

g                                                                       61


<210> 48
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 48
cgttaaatgc acggttgggg atgtgagact acagtctcat ttcgccaacg gccttttc         58


<210> 49
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 49
cgttaaacac cgccattccg ggcgatccgc gtgataccac ttcacctcgg gc               52


<210> 50
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 50
ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa       60


<210> 51
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 51
gcgtcaaatc gacactaccc gacatgttga gttttattca tgccaacctt aacccacaga       60


<210> 52
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 52
tccaattcgt ccgcgctatt gacttttttc acaccgaagg atgagcctga ac               52


<210> 53
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> See specification as filed for detailed description of 
	substitutions and preferred embodiments 

<400> 53
ggtaaggttc ttcgcgttgc wtcgaattaa accacatgct cca                         43


<210> 54
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 54
ggagcatgtg gtttaattcg a                                                 21


<210> 55
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 55
ggtaaggttc ttcgcgtt                                                     18


<210> 56
<211> 48
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 56
catgaatcta ctcgacgata ttgctcaact gtcataaact tctgagga                    48


<210> 57
<211> 13
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 57
tcctcagaag ttt                                                          13


<210> 58
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 58
tatcgtcgag tagattcatg                                                   20


<210> 59
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 59
tgcgggtaac gtcaatgagc aaaggtatta actttactcc cttcctcca                   49


<210> 60
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 60
ggaggaaggg agtaaagtta atacctttg                                         29


<210> 61
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 61
tgcgggtaac gtcaatgag                                                    19


<210> 62
<211> 75
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 62
acggcagtta gccggtgctt cttctgcggg taacgtccaa tcgccaaggt tattaacctt       60

atcgccttgc ctcca                                                        75


<210> 63
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 63
ggaggcaagg cgataaggt                                                    19


<210> 64
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 64
acggcagtta gccggtgctt ct                                                22


<210> 65
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 65
gagttagccg gtgcttattc tgcgagtaac gtccactatc cctaggtatt aactagaa         58


<210> 66
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 66
tctagttaat acctagggat agtggacgtt                                        30


<210> 67
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 67
gagttagccg gtgcttattc tgcgag                                            26


<210> 68
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 68
tattctgttg gtaacgtcaa aacagcaagg tattaactta ctgcccttcc tcca             54


<210> 69
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 69
ggaggaaggg cagtaagtta ataccttg                                          28


<210> 70
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 70
gcttattctg ttggtaacgt caaaacag                                          28


<210> 71
<211> 71
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 71
gtagttagcc gtggctttct gattaggtac cgtcaagatg tgcacagtta cttacacata       60

tgttcttccc a                                                            71


<210> 72
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 72
gggaagaaca tatgtgtaag taac                                              24


<210> 73
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 73
gtagttagcc gtggctttct                                                   20


<210> 74
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 74
cacctgaaac aaagcatcct aaaaaaggtg tagagaaata tggtcctgaa gcaa             54


<210> 75
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 75
tgcttcagga ccatatttct ctac                                              24


<210> 76
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 76
cacctgaaac aaagcatcct aaa                                               23


<210> 77
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 77
tgaattgagc aaaacggacg gcccaattct aaaccttgct tctgga                      46


<210> 78
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 78
ccagaagcaa ggtttagaat tg                                                22


<210> 79
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 79
tgaattgagc aaaacggacg gc                                                22


<210> 80
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 80
atgaaggtgt gcttacaagt gctaataatt cacctgtttg agggtggata gcaga            55


<210> 81
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 81
ctgctatcca ccctcaaaca gg                                                22


<210> 82
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 82
atgaaggtgt gcttacaagt gc                                                22


<210> 83
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 83
cacctgataa gccgttagag atattaatat ctccaccata agaataacct accga            55


<210> 84
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 84
cggtaggtta ttcttatggt ggagat                                            26


<210> 85
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 85
cacctgataa gccgttagag atatt                                             25


<210> 86
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (21)..(21)
<223> a, c, t, g, unknown or other

<400> 86
ggtaaggttc ttcgcgttgc ntcgaattaa accacatgct cca                         43


<210> 87
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 87
caatgtacat tattgtggag catgtggttt aattcga                                37


<210> 88
<211> 13
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 88
tcctcagaag ttt                                                          13


<210> 89
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 89
caatgtacat tattgtggag gaagggagta aagttaat                               38


<210> 90
<211> 51
<212> DNA
<213> Escherichia coli

<400> 90
tctgcgggta acgtcaatga gcaaaggtat taactttact cccttcctcc a                51


<210> 91
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 91
acttgtggta gttggagctg a                                                 21


<210> 92
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 92
acttgtggta gttggagccg a                                                 21


<210> 93
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 93
atgattctga attagctgta tcgt                                              24


<210> 94
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 94
acttgtggta gttggagctg t                                                 21


<210> 95
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 95
acttgtggta gttggagccg t                                                 21




