                         SEQUENCE LISTING

<110>  Avalos, Jose
       Toettcher, Jared
       Zhao, Evan
 
<120>  SYSTEM AND METHOD OF OPTOGENETICALLY CONTROLLING METABOLIC 
       PATHWAYS FOR THE PRODUCTION OF CHEMICALS

<130>  16-3251 / PRIN-49576

<140>  PCT/US2017/026615
<141>  2017-04-07

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Minimal Promoter Sequence

<400>  1

Thr Ala Gly Ala Gly Gly Gly Thr Ala Thr Ala Thr Ala Ala Thr Gly 
1               5                   10                  15      


Gly Ala Ala Gly Cys Thr Cys Gly Ala Cys Thr Thr Cys Cys Ala Gly 
            20                  25                  30          


<210>  2
<211>  178
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CLN2 PEST tag

<400>  2

Ala Ser Asn Leu Asn Ile Ser Arg Lys Leu Thr Ile Ser Thr Pro Ser 
1               5                   10                  15      


Cys Ser Phe Glu Asn Ser Asn Ser Thr Ser Ile Pro Ser Pro Ala Ser 
            20                  25                  30          


Ser Ser Gln Ser His Thr Pro Met Arg Asn Met Ser Ser Leu Ser Asp 
        35                  40                  45              


Asn Ser Val Phe Ser Arg Asn Met Glu Gln Ser Ser Pro Ile Thr Pro 
    50                  55                  60                  


Ser Met Tyr Gln Phe Gly Gln Gln Gln Ser Asn Ser Ile Cys Gly Ser 
65                  70                  75                  80  


Thr Val Ser Val Asn Ser Leu Val Asn Thr Asn Asn Lys Gln Arg Ile 
                85                  90                  95      


Tyr Glu Gln Ile Thr Gly Pro Asn Ser Asn Asn Ala Thr Asn Asp Tyr 
            100                 105                 110         


Ile Asp Leu Leu Asn Leu Asn Glu Ser Asn Lys Glu Asn Gln Asn Pro 
        115                 120                 125             


Ala Thr Ala His Tyr Leu Asn Gly Gly Pro Pro Lys Thr Ser Phe Ile 
    130                 135                 140                 


Asn His Gly Met Phe Pro Ser Pro Thr Gly Thr Ile Asn Ser Gly Lys 
145                 150                 155                 160 


Ser Ser Ser Ala Ser Ser Leu Ile Ser Phe Gly Met Gly Asn Thr Gln 
                165                 170                 175     


Val Ile 
        


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CL1 mutant tag

<300>
<301>  Cilon, Chomsky, Kulka
<302>  Degradation Signals Recognized by the Ubc6p-Ubc7p 
       Ubiquitin-Conjugating Enzyme Pair
<303>  Molecular and Cellular Biology
<304>  20
<306>  7214-7219
<307>  2000-10   

<300>
<301>  Gilon, Tamar
<302>  Degradation Signals Recognized by the Ubc6p-Ubc7p 
       Ubiquitin-Conjugating Enzyme Pair
<303>  Molecular and Cellular Biology
<304>  20
<306>  7214-7219
<307>  2000-10   

<400>  3

Ala Cys Lys Asn Trp Phe Ser Ser Leu Ser Ala Phe Val Ile Ala Leu 
1               5                   10                  15      


<210>  4
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ODC mutant tag

<300>
<301>  Takeuchi, Chen, Hoyt, Coffino
<302>  Structural elements of the ubiquitin-independent proteasome 
       degron of ornithine decarboxylase
<303>  Biochemical Journal
<304>  410
<305>  2
<306>  401-407
<307>  2008-03-01

<300>
<301>  Takeuchi, Junko
<302>  Structural elements of the ubiquitin-independent proteasome 
       degron of ornithine decarboxylase
<303>  Biochemical Journal
<304>  410
<305>  2
<306>  401-407
<307>  2008-03-01

<400>  4

Phe Pro Pro Glu Val Glu Glu Gln Asp Asp Gly Thr Leu Pro Met Ser 
1               5                   10                  15      


Cys Ala Gln Glu Ser Gly Met Asp Arg His Pro Ala Ser Cys Pro Glu 
            20                  25                  30          


Arg Ala Ala Cys Ala Ser Ala Arg Ile Asn Val 
        35                  40              


<210>  5
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGAL1 mutant

<400>  5

Ala Gly Cys Thr Gly Gly Ala Gly Cys Thr Cys Ala Cys Cys Gly Gly 
1               5                   10                  15      


Thr Ala Thr Ala Cys Cys Cys Gly Gly Gly Cys Gly Gly Ala Thr Thr 
            20                  25                  30          


Ala Gly Ala Ala Gly Cys Cys Gly Cys Cys Gly Ala Gly Cys Gly Gly 
        35                  40                  45              


Gly Thr Gly Ala Cys Ala Gly Cys Cys Cys Thr Cys Cys Gly Ala Ala 
    50                  55                  60                  


Gly Gly Ala Ala Gly Ala Cys Thr Cys Thr Cys Cys Thr Cys Cys Gly 
65                  70                  75                  80  


Thr Gly Cys Gly Thr Cys Cys Thr Cys Gly Thr Cys Thr Thr Cys Ala 
                85                  90                  95      


Cys Cys Gly Gly Thr Cys Gly Cys Gly Thr Thr Cys Cys Thr Gly Ala 
            100                 105                 110         


Ala Ala Cys Gly Cys Ala Gly Ala Thr Gly Thr Gly Cys Cys Thr Cys 
        115                 120                 125             


Gly Cys Gly Cys Cys Gly Cys Ala Cys Thr Gly Cys Thr Cys Cys Gly 
    130                 135                 140                 


Ala Ala Cys Ala Ala Thr Ala Ala Ala Gly Ala Thr Thr Cys Thr Ala 
145                 150                 155                 160 


Cys Ala Ala Thr Ala Cys Thr Ala Gly Cys Thr Thr Thr Thr Ala Thr 
                165                 170                 175     


Gly Gly Thr Thr Ala Thr Gly Ala Ala Gly Ala Gly Gly Ala Ala Ala 
            180                 185                 190         


Ala Ala Thr Thr Gly Gly Cys Ala Gly Thr Ala Ala Cys Cys Thr Gly 
        195                 200                 205             


Gly Thr Thr Gly Gly Thr Ala Ala Ala Ala Cys Cys Thr Thr Cys Ala 
    210                 215                 220                 


Ala Ala Thr Gly Ala Ala Cys Gly Ala Ala Thr Cys Ala Ala Ala Thr 
225                 230                 235                 240 


Thr Ala Ala Cys Ala Ala Cys Cys Ala Thr Ala Gly Gly Ala Thr Gly 
                245                 250                 255     


Ala Thr Ala Ala Thr Gly Cys Gly Ala Thr Thr Ala Gly Thr Thr Thr 
            260                 265                 270         


Thr Thr Thr Ala Gly Cys Cys Thr Thr Ala Thr Thr Thr Thr Ala Gly 
        275                 280                 285             


Thr Ala Gly Thr Ala Ala Thr Thr Ala Ala Thr Cys Ala Gly Cys Gly 
    290                 295                 300                 


Ala Ala Gly Cys Gly Ala Thr Gly Ala Thr Thr Thr Thr Thr Gly Ala 
305                 310                 315                 320 


Thr Cys Thr Ala Thr Thr Ala Ala Cys Ala Gly Ala Thr Ala Thr Ala 
                325                 330                 335     


Thr Ala Ala Ala Thr Gly Cys Ala Ala Ala Ala Ala Cys Thr Gly Cys 
            340                 345                 350         


Ala Thr Ala Ala Cys Cys Ala Cys Thr Thr Thr Ala Ala Cys Thr Ala 
        355                 360                 365             


Ala Thr Ala Cys Thr Thr Thr Cys Ala Ala Cys Ala Thr Thr Thr Thr 
    370                 375                 380                 


Cys Gly Gly Thr Thr Thr Gly Thr Ala Thr Thr Ala Cys Thr Thr Cys 
385                 390                 395                 400 


Thr Thr Ala Thr Thr Cys Ala Ala Ala Thr Gly Thr Ala Ala Thr Ala 
                405                 410                 415     


Ala Ala Ala Gly Thr Ala Thr Cys Ala Ala Cys Ala Ala Ala Ala Ala 
            420                 425                 430         


Ala Thr Thr Gly Thr Thr Ala Ala Thr Ala Thr Ala Cys Cys Thr Cys 
        435                 440                 445             


Thr Ala Thr Ala Cys Thr Thr Thr Ala Ala Cys Gly Thr Cys Ala Ala 
    450                 455                 460                 


Gly Gly Ala Gly Ala Ala Ala Ala Ala Ala Cys Thr Ala Thr Ala Gly 
465                 470                 475                 480 


Cys Gly Gly Cys Cys Gly Cys Thr Ala Ala Ala Ala Thr Cys Ala Thr 
                485                 490                 495     


Gly Gly Cys 
            


<210>  6
<211>  699
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PGAL1 mutant

<400>  6

Ala Gly Cys Thr Cys Ala Cys Cys Gly Gly Thr Ala Thr Ala Cys Cys 
1               5                   10                  15      


Cys Gly Gly Gly Cys Gly Gly Ala Thr Thr Ala Gly Ala Ala Gly Cys 
            20                  25                  30          


Cys Gly Cys Cys Gly Ala Gly Cys Gly Gly Gly Thr Gly Ala Cys Ala 
        35                  40                  45              


Gly Cys Cys Cys Thr Cys Cys Gly Ala Ala Gly Gly Ala Ala Gly Ala 
    50                  55                  60                  


Cys Thr Cys Thr Cys Cys Thr Cys Cys Gly Thr Gly Cys Gly Thr Cys 
65                  70                  75                  80  


Cys Thr Cys Gly Thr Cys Thr Thr Cys Ala Cys Cys Gly Gly Thr Cys 
                85                  90                  95      


Gly Cys Gly Thr Thr Cys Cys Thr Gly Ala Ala Ala Cys Gly Cys Ala 
            100                 105                 110         


Gly Ala Thr Gly Thr Gly Cys Cys Thr Cys Gly Cys Gly Cys Cys Gly 
        115                 120                 125             


Cys Ala Cys Thr Gly Cys Thr Cys Cys Gly Ala Ala Cys Ala Ala Thr 
    130                 135                 140                 


Ala Ala Ala Gly Ala Thr Thr Cys Thr Ala Cys Ala Ala Thr Ala Cys 
145                 150                 155                 160 


Thr Ala Gly Cys Thr Thr Thr Thr Ala Thr Gly Gly Thr Thr Ala Thr 
                165                 170                 175     


Gly Ala Ala Gly Ala Gly Gly Ala Ala Ala Ala Ala Thr Thr Gly Gly 
            180                 185                 190         


Ala Thr Gly Ala Thr Thr Thr Thr Thr Gly Ala Thr Cys Thr Ala Thr 
        195                 200                 205             


Thr Ala Ala Cys Ala Gly Ala Thr Ala Thr Ala Thr Ala Ala Ala Thr 
    210                 215                 220                 


Gly Cys Ala Ala Ala Ala Ala Cys Gly Gly Ala Thr Thr Ala Gly Ala 
225                 230                 235                 240 


Ala Gly Cys Cys Gly Cys Cys Gly Ala Gly Cys Gly Gly Gly Thr Gly 
                245                 250                 255     


Ala Cys Ala Gly Cys Cys Cys Thr Cys Cys Gly Ala Ala Gly Gly Ala 
            260                 265                 270         


Ala Gly Ala Cys Thr Cys Thr Cys Cys Thr Cys Cys Gly Thr Gly Cys 
        275                 280                 285             


Gly Thr Cys Cys Thr Cys Gly Thr Cys Thr Thr Cys Ala Cys Cys Gly 
    290                 295                 300                 


Gly Thr Cys Gly Cys Gly Thr Thr Cys Cys Thr Gly Ala Ala Ala Cys 
305                 310                 315                 320 


Gly Cys Ala Gly Ala Thr Gly Thr Gly Cys Cys Thr Cys Gly Cys Gly 
                325                 330                 335     


Cys Cys Gly Cys Ala Cys Thr Gly Cys Thr Cys Cys Gly Ala Ala Cys 
            340                 345                 350         


Ala Ala Thr Ala Ala Ala Gly Ala Thr Thr Cys Thr Ala Cys Ala Ala 
        355                 360                 365             


Thr Ala Cys Thr Ala Gly Cys Thr Thr Thr Thr Ala Thr Gly Gly Thr 
    370                 375                 380                 


Thr Ala Thr Gly Ala Ala Gly Ala Gly Gly Ala Ala Ala Ala Ala Thr 
385                 390                 395                 400 


Thr Gly Gly Cys Ala Gly Thr Ala Ala Cys Cys Thr Gly Gly Thr Thr 
                405                 410                 415     


Gly Gly Thr Ala Ala Ala Ala Cys Cys Thr Thr Cys Ala Ala Ala Thr 
            420                 425                 430         


Gly Ala Ala Cys Gly Ala Ala Thr Cys Ala Ala Ala Thr Thr Ala Ala 
        435                 440                 445             


Cys Ala Ala Cys Cys Ala Thr Ala Gly Gly Ala Thr Gly Ala Thr Ala 
    450                 455                 460                 


Ala Thr Gly Cys Gly Ala Thr Thr Ala Gly Thr Thr Thr Thr Thr Thr 
465                 470                 475                 480 


Ala Gly Cys Cys Thr Thr Ala Thr Thr Thr Thr Ala Gly Thr Ala Gly 
                485                 490                 495     


Thr Ala Ala Thr Thr Ala Ala Thr Cys Ala Gly Cys Gly Ala Ala Gly 
            500                 505                 510         


Cys Gly Ala Thr Gly Ala Thr Thr Thr Thr Thr Gly Ala Thr Cys Thr 
        515                 520                 525             


Ala Thr Thr Ala Ala Cys Ala Gly Ala Thr Ala Thr Ala Thr Ala Ala 
    530                 535                 540                 


Ala Thr Gly Cys Ala Ala Ala Ala Ala Cys Thr Gly Cys Ala Thr Ala 
545                 550                 555                 560 


Ala Cys Cys Ala Cys Thr Thr Thr Ala Ala Cys Thr Ala Ala Thr Ala 
                565                 570                 575     


Cys Thr Thr Thr Cys Ala Ala Cys Ala Thr Thr Thr Thr Cys Gly Gly 
            580                 585                 590         


Thr Thr Thr Gly Thr Ala Thr Thr Ala Cys Thr Thr Cys Thr Thr Ala 
        595                 600                 605             


Thr Thr Cys Ala Ala Ala Thr Gly Thr Ala Ala Thr Ala Ala Ala Ala 
    610                 615                 620                 


Gly Thr Ala Thr Cys Ala Ala Cys Ala Ala Ala Ala Ala Ala Thr Thr 
625                 630                 635                 640 


Gly Thr Thr Ala Ala Thr Ala Thr Ala Cys Cys Thr Cys Thr Ala Thr 
                645                 650                 655     


Ala Cys Thr Thr Thr Ala Ala Cys Gly Thr Cys Ala Ala Gly Gly Ala 
            660                 665                 670         


Gly Ala Ala Ala Ala Ala Ala Cys Thr Ala Thr Ala Gly Cys Gly Gly 
        675                 680                 685             


Cys Cys Gly Cys Thr Ala Ala Ala Ala Thr Cys 
    690                 695                 


